MD04G1209800.v1.1

39S ribosomal protein L45

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0013393
Physical Location & Seq
Forward (+)
29467065 .. 29469794
2730 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1209800.v1.1.491

Sequence Viewer

Length: 714 bp
ATGGCTTTCAGAAGCTTACACCAGATTCGAGCTCTCTATCGTACGGCCCAGATTGCAGAATCCTCCCATCTGCTTGGAAGCTCAAGAAACTATGCAACTGGTATTTCAAATGTCCCGGAATCTTATAGCCGGACTGTATCGTCTTGCTTTTACGAGGGCAGCCATGGGAGCCTTCCTTGGAGTCACGGAAGTTCCATGACATTGCGTTCTGCAATGGCGGTTGAGTTGCCGCTCTTCTTGAGTGACACAAGATCACTATCAACACAAGTAAAAGCGCCTGCACAAGCAAGACAAATGGGTGCTCTTAGAGTAGCCATGTTGAGTTCAGGGTTTATCTATGAGCCTTATACACCTCATGAAAAAATCTCATTTTTCAAGAGATGGTTCACAAGAAGCGGGTGCAAAAGAACAAAAGAAGACACAATAATAGAGCTCAAAAATGCATATGCCATTTCTAAATTAAGAAAAAAGGGGTATTCAAAGAATCTATTCTACAAAGAAGCAATAAACTTATACAAGGAGACCTTGCGTAAAGCGGGAGCCTTGTGCACTGGGTACGACGATCTACGACGATCTAGCGTCTTTTCGTCCTTTCAAGTTTATAAATACTCTCTAATGGCAAATGGGGACAAAAATTCTTTAAGGAAAGCAGTTACGGAGAAAATGTTTTCGGAACTCAAGAATGATCAAACAAAGAGATCCAATATGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tim44

Protein Analysis

238

Amino Acids

26.92

Weight (kDa)

9.9

Isoelectric Point (pI)

54.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013393)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G27395 AT5G27395
fragaria_vesca FvH4_6g05290
malus_domestica MD04G1209800.v1.1 MD12G1224400.v1.1
prunus_persica Prupe.6G330700_v2.0.a1 Prupe.6G330700_v2.0.a1
pyrus_communis pycom04g18600 pycom12g21000
rosa_chinensis RchiOBHm_Chr3g0454031
rosa_laevigata RLG00000025488
rosa_multiflora Rmu_sc0019265.1_g000003 Rmu_sc0020385.1_g000006
rosa_rugosa Rorug02G0652400
rosa_samantha Rh3AG055100 Rh3BG056700 Rh3DG057200
rosa_wichuraiana Rw3G004230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 603
AasI GACNNNNNNGTC 1 cut(s) 139
AccBSI CCGCTC 1 cut(s) 232
AciI CCGC 4 cut(s) 218, 230, 396, 536
AclWI GGATC 1 cut(s) 693
AcsI RAATTY 1 cut(s) 634
AfaI GTAC 2 cut(s) 43, 557
AgsI TTSAA 4 cut(s) 108, 376, 480, 596
AluBI AGCT 4 cut(s) 15, 32, 81, 433
AluI AGCT 4 cut(s) 15, 32, 81, 433
Alw21I GWGCWC 4 cut(s) 34, 304, 435, 551
Alw26I GTCTC 1 cut(s) 515
Alw44I GTGCAC 1 cut(s) 547
AlwI GGATC 1 cut(s) 693
AoxI GGCC 1 cut(s) 45
ApaLI GTGCAC 1 cut(s) 547
ApeKI GCWGC 1 cut(s) 159
ApoI RAATTY 1 cut(s) 634
Asp700I GAANNNNTTC 1 cut(s) 488
AspLEI GCGC 1 cut(s) 277
AspS9I GGNCC 1 cut(s) 46
AsuC2I CCSGG 1 cut(s) 116
BaeGI GKGCMC 1 cut(s) 551
BanII GRGCYC 2 cut(s) 34, 435
BbsI GAAGAC 1 cut(s) 423
Bbv12I GWGCWC 4 cut(s) 34, 304, 435, 551
BbvI GCAGC 1 cut(s) 171
BccI CCATC 2 cut(s) 75, 375
BceAI ACGGC 1 cut(s) 60
BclI TGATCA 1 cut(s) 685
BcnI CCSGG 1 cut(s) 116
BcoDI GTCTC 1 cut(s) 515
BfaI CTAG 1 cut(s) 576
BfoI RGCGCY 1 cut(s) 278
BisI GCNGC 2 cut(s) 160, 230
BlsI GCNGC 2 cut(s) 161, 231
Bme1390I CCNGG 1 cut(s) 116
BmgT120I GGNCC 1 cut(s) 46
BmiI GGNNCC 2 cut(s) 170, 541
BmrFI CCNGG 1 cut(s) 116
BmrI ACTGGG 1 cut(s) 561
BmuI ACTGGG 1 cut(s) 561
BpiI GAAGAC 1 cut(s) 423
BpuEI CTTGAG 3 cut(s) 67, 259, 662
BpuMI CCSGG 1 cut(s) 116
BsaBI GATNNNNATC 1 cut(s) 256
BsaI GGTCTC 1 cut(s) 515
BsaJI CCNNGG 2 cut(s) 163, 176
BsaXI ACNNNNNCTCC 2 cut(s) 650, 680
Bse1I ACTGG 2 cut(s) 103, 556
Bse3DI GCAATG 2 cut(s) 200, 219
Bse8I GATNNNNATC 1 cut(s) 256
BseDI CCNNGG 2 cut(s) 163, 176
BseJI GATNNNNATC 1 cut(s) 256
BseMI GCAATG 2 cut(s) 200, 219
BseNI ACTGG 2 cut(s) 103, 556
BseSI GKGCMC 1 cut(s) 551
BseXI GCAGC 1 cut(s) 171
BsgI GTGCAG 1 cut(s) 264
BshFI GGCC 1 cut(s) 47
BsiHKAI GWGCWC 4 cut(s) 34, 304, 435, 551
BsiSI CCGG 2 cut(s) 116, 130
BsiWI CGTACG 1 cut(s) 41
BslFI GGGAC 2 cut(s) 98, 641
BsmAI GTCTC 1 cut(s) 515
BsmFI GGGAC 2 cut(s) 98, 641
BsnI GGCC 1 cut(s) 47
Bso31I GGTCTC 1 cut(s) 515
Bsp1286I GDGCHC 4 cut(s) 34, 304, 435, 551
Bsp143I GATC 5 cut(s) 251, 562, 572, 685, 698
Bsp19I CCATGG 1 cut(s) 163
BspACI CCGC 4 cut(s) 218, 230, 396, 536
BspANI GGCC 1 cut(s) 47
BspHI TCATGA 1 cut(s) 355
BspLI GGNNCC 2 cut(s) 170, 541
BspPI GGATC 1 cut(s) 693
BspQI GCTCTTC 1 cut(s) 239
BspTNI GGTCTC 1 cut(s) 515
BsrBI CCGCTC 1 cut(s) 232
BsrDI GCAATG 2 cut(s) 200, 219
BsrI ACTGG 2 cut(s) 103, 556
BssECI CCNNGG 2 cut(s) 163, 176
BssMI GATC 5 cut(s) 251, 562, 572, 685, 698
BssT1I CCWWGG 2 cut(s) 163, 176
Bst4CI ACNGT 1 cut(s) 136
Bst6I CTCTTC 1 cut(s) 239
BstC8I GCNNGC 1 cut(s) 279
BstDEI CTNAG 1 cut(s) 305
BstDSI CCRYGG 1 cut(s) 163
BstH2I RGCGCY 1 cut(s) 278
BstHHI GCGC 1 cut(s) 277
BstKTI GATC 5 cut(s) 254, 565, 575, 688, 701
BstMAI GTCTC 1 cut(s) 515
BstMBI GATC 5 cut(s) 251, 562, 572, 685, 698
BstMWI GCNNNNNNNGC 2 cut(s) 53, 168
BstSCI CCNGG 1 cut(s) 114
BstSLI GKGCMC 1 cut(s) 551
BstV1I GCAGC 1 cut(s) 171
BstV2I GAAGAC 1 cut(s) 423
BstX2I RGATCY 1 cut(s) 698
BstXI CCANNNNNNTGG 1 cut(s) 74
BstYI RGATCY 1 cut(s) 698
BsuRI GGCC 1 cut(s) 47
BtgI CCRYGG 1 cut(s) 163
BtsIMutI CAGTG 1 cut(s) 549
Cac8I GCNNGC 1 cut(s) 279
CciI TCATGA 1 cut(s) 355
CfoI GCGC 1 cut(s) 277
Cfr13I GGNCC 1 cut(s) 46
CseI GACGC 1 cut(s) 568
Csp6I GTAC 2 cut(s) 42, 556
CviAII CATG 4 cut(s) 164, 196, 316, 356
CviQI GTAC 2 cut(s) 42, 556
DdeI CTNAG 1 cut(s) 305
DpnI GATC 5 cut(s) 253, 564, 574, 687, 700
DpnII GATC 5 cut(s) 251, 562, 572, 685, 698
DrdI GACNNNNNNGTC 1 cut(s) 139
DseDI GACNNNNNNGTC 1 cut(s) 139
Eam1104I CTCTTC 1 cut(s) 239
EarI CTCTTC 1 cut(s) 239
Ecl136II GAGCTC 2 cut(s) 32, 433
Eco130I CCWWGG 2 cut(s) 163, 176
Eco24I GRGCYC 2 cut(s) 34, 435
Eco31I GGTCTC 1 cut(s) 515
Eco53kI GAGCTC 2 cut(s) 32, 433
EcoICRI GAGCTC 2 cut(s) 32, 433
EcoT14I CCWWGG 2 cut(s) 163, 176
EcoT22I ATGCAT 1 cut(s) 445
EcoT38I GRGCYC 2 cut(s) 34, 435
ErhI CCWWGG 2 cut(s) 163, 176
FaeI CATG 4 cut(s) 167, 199, 319, 359
FalI AAGNNNNNCTT 2 cut(s) 509, 541
FaqI GGGAC 2 cut(s) 98, 641
FatI CATG 4 cut(s) 163, 195, 315, 355
FauI CCCGC 2 cut(s) 389, 529
FauNDI CATATG 1 cut(s) 445
FbaI TGATCA 1 cut(s) 685
Fnu4HI GCNGC 2 cut(s) 160, 230
FriOI GRGCYC 2 cut(s) 34, 435
Fsp4HI GCNGC 2 cut(s) 160, 230
FspBI CTAG 1 cut(s) 576
GlaI GCGC 1 cut(s) 276
GluI GCNGC 2 cut(s) 160, 230
HaeII RGCGCY 1 cut(s) 278
HaeIII GGCC 1 cut(s) 47
HapII CCGG 2 cut(s) 116, 130
HgaI GACGC 1 cut(s) 568
HhaI GCGC 1 cut(s) 277
Hin1II CATG 4 cut(s) 167, 199, 319, 359
Hin6I GCGC 1 cut(s) 275
HinP1I GCGC 1 cut(s) 275
HindIII AAGCTT 1 cut(s) 13
HinfI GANTC 5 cut(s) 25, 59, 119, 181, 484
HpaII CCGG 2 cut(s) 116, 130
Hpy166II GTNNAC 2 cut(s) 387, 549
Hpy188I TCNGA 2 cut(s) 11, 673
Hpy188III TCNNGA 5 cut(s) 84, 238, 356, 376, 679
Hpy8I GTNNAC 2 cut(s) 387, 549
Hpy99I CGWCG 2 cut(s) 563, 573
HpyAV CCTTC 1 cut(s) 182
HpyCH4III ACNGT 1 cut(s) 136
HpyCH4V TGCA 7 cut(s) 56, 95, 212, 281, 402, 443, 549
HpyF10VI GCNNNNNNNGC 2 cut(s) 53, 168
HpyF3I CTNAG 1 cut(s) 305
Hsp92II CATG 4 cut(s) 167, 199, 319, 359
HspAI GCGC 1 cut(s) 275
Ksp22I TGATCA 1 cut(s) 685
Kzo9I GATC 5 cut(s) 251, 562, 572, 685, 698
LguI GCTCTTC 1 cut(s) 239
LmnI GCTCC 2 cut(s) 168, 539
LpnPI CCDG 8 cut(s) 35, 62, 84, 129, 143, 291, 312, 537
Lsp1109I GCAGC 1 cut(s) 171
MaeI CTAG 1 cut(s) 576
MaeIII GTNAC 3 cut(s) 182, 242, 652
MalI GATC 5 cut(s) 253, 564, 574, 687, 700
MbiI CCGCTC 1 cut(s) 232
MboI GATC 5 cut(s) 251, 562, 572, 685, 698
MboII GAAGA 2 cut(s) 226, 428
MflI RGATCY 1 cut(s) 698
MhlI GDGCHC 4 cut(s) 34, 304, 435, 551
MluCI AATT 2 cut(s) 458, 634
MlyI GAGTC 1 cut(s) 190
MnlI CCTC 3 cut(s) 73, 148, 363
Mph1103I ATGCAT 1 cut(s) 445
MroXI GAANNNNTTC 1 cut(s) 488
MseI TTAA 2 cut(s) 461, 641
MspI CCGG 2 cut(s) 116, 130
MspR9I CCNGG 1 cut(s) 116
MwoI GCNNNNNNNGC 2 cut(s) 53, 168
NciI CCSGG 1 cut(s) 116
NcoI CCATGG 1 cut(s) 163
NdeI CATATG 1 cut(s) 445
NdeII GATC 5 cut(s) 251, 562, 572, 685, 698
NlaIII CATG 4 cut(s) 167, 199, 319, 359
NlaIV GGNNCC 2 cut(s) 170, 541
NmuCI GTSAC 2 cut(s) 182, 242
NsiI ATGCAT 1 cut(s) 445
PagI TCATGA 1 cut(s) 355
PciSI GCTCTTC 1 cut(s) 239
PdmI GAANNNNTTC 1 cut(s) 488
PfeI GAWTC 4 cut(s) 25, 59, 119, 484
Pfl23II CGTACG 1 cut(s) 41
PfoI TCCNGGA 1 cut(s) 114
PkrI GCNGC 2 cut(s) 161, 231
PleI GAGTC 1 cut(s) 189
PpsI GAGTC 1 cut(s) 189
PsiI TTATAA 1 cut(s) 603
Psp124BI GAGCTC 2 cut(s) 34, 435
PspLI CGTACG 1 cut(s) 41
PspN4I GGNNCC 2 cut(s) 170, 541
PspPI GGNCC 1 cut(s) 46
PsuI RGATCY 1 cut(s) 698
RsaI GTAC 2 cut(s) 43, 557
RsaNI GTAC 2 cut(s) 42, 556
SacI GAGCTC 2 cut(s) 34, 435
SapI GCTCTTC 1 cut(s) 239
SaqAI TTAA 2 cut(s) 461, 641
SatI GCNGC 2 cut(s) 160, 230
Sau3AI GATC 5 cut(s) 251, 562, 572, 685, 698
Sau96I GGNCC 1 cut(s) 46
SchI GAGTC 1 cut(s) 190
ScrFI CCNGG 1 cut(s) 116
SduI GDGCHC 4 cut(s) 34, 304, 435, 551
SetI ASST 6 cut(s) 17, 34, 83, 355, 435, 527
SmlI CTYRAG 3 cut(s) 82, 238, 677
SmoI CTYRAG 3 cut(s) 82, 238, 677
Sse9I AATT 2 cut(s) 458, 634
SsiI CCGC 4 cut(s) 218, 230, 396, 536
SspMI CTAG 1 cut(s) 576
SstI GAGCTC 2 cut(s) 34, 435
StyD4I CCNGG 1 cut(s) 114
StyI CCWWGG 2 cut(s) 163, 176
TaaI ACNGT 1 cut(s) 136
TaqI TCGA 1 cut(s) 28
TasI AATT 2 cut(s) 458, 634
TauI GCSGC 1 cut(s) 232
TfiI GAWTC 4 cut(s) 25, 59, 119, 484
Tru1I TTAA 2 cut(s) 461, 641
Tru9I TTAA 2 cut(s) 461, 641
TscAI CASTG 1 cut(s) 556
TseFI GTSAC 2 cut(s) 182, 242
TseI GCWGC 1 cut(s) 159
Tsp45I GTSAC 2 cut(s) 182, 242
TspDTI ATGAA 1 cut(s) 372
TspGWI ACGGA 2 cut(s) 201, 671
TspRI CASTG 1 cut(s) 556
VneI GTGCAC 1 cut(s) 547
XapI RAATTY 1 cut(s) 634
XmnI GAANNNNTTC 1 cut(s) 488
XspI CTAG 1 cut(s) 576
Zsp2I ATGCAT 1 cut(s) 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.