Rw3G004230

39S ribosomal protein L45

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0013393
Physical Location & Seq
Reverse (-)
3701436 .. 3705337
3902 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G004230.1

Sequence Viewer

Length: 906 bp
ATGATGGCTTTCAGAAGCTCAAACACGATTCGTGTTCTCTTGCGTGCGACCCAGATTCAAGAATCTTCGTATCTTCTTGGATGCTCAAGAAGCTACGGAACTGGTATTTCAAATGTGGCCGAACTTAATAGCCGGAACATGTACTCAAGCTTATATAAGGGCAATGGAGCACTTCCTTGGACTTGTAGAAATGCTATGACGCTGCGTTCTGCAATGGCTGTCGAGTTGCCAATCTTCTTGAATGATAAAAGGTTGCTATCCACACAAGTAAAAGCACCTGCCCAAGCAAGACAAATGGGAGCACTTAGAGTTTCCATGACAAGCCCAGGATTTATCTATGAGCCTTATGCACCTCGTGAAAAAGTTCCATTTTTCCAGAGATGGTTCACTAGAAGTGGCTGGAGAAGAACAAAAGAAGATATAATTTTAGAGCTTAAAAGTGCATATGCTATTAATAAATTAAGAAAGTCGGGGTATTCAAAGAATCTATTCTACAAAGAAGCAATCAATGTATACAAGGAGATAAATACTCTATTGGCAACTGGAGACAAAAACACTTTAAGGAAAGCAGTTACGGAGAAAATGTATTCTGAACTAAAGAATGAAATAAAACAGAGAGAATCCATATGGAGTAAGGTTTATTGGGAAATGGTGGAGCCAATTGTAAAAATACGCACCTTGCGAGCACGACTGATTGGTGTTGATCCAAAAGATCTTGAAAAGGTGTTTATACAGCTTACACTTGAGTTTCTGTCAAAGCAGAAGTTTGAAGCATATGACTCAAAGGGTTCTGTTGTTGCTGGAGATAAATCTAAGGAGGTTCTTGTCCGTGATATCTGGGTATTTGAAAAGTCTCTCTTCCATCCTAAATCTTTCTGGCGTCTTTGTGGGAGGATTACTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tim44

Protein Analysis

301

Amino Acids

34.82

Weight (kDa)

9.96

Isoelectric Point (pI)

40.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim44 PF04280 147 - 297 2.1e-22 Tim44-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013393)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G27395 AT5G27395
fragaria_vesca FvH4_6g05290
malus_domestica MD04G1209800.v1.1 MD12G1224400.v1.1
prunus_persica Prupe.6G330700_v2.0.a1 Prupe.6G330700_v2.0.a1
pyrus_communis pycom04g18600 pycom12g21000
rosa_chinensis RchiOBHm_Chr3g0454031
rosa_laevigata RLG00000025488
rosa_multiflora Rmu_sc0019265.1_g000003 Rmu_sc0020385.1_g000006
rosa_rugosa Rorug02G0652400
rosa_samantha Rh3AG055100 Rh3BG056700 Rh3DG057200
rosa_wichuraiana Rw3G004230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 286
Acc36I ACCTGC 1 cut(s) 286
AccI GTMKAC 1 cut(s) 513
AclWI GGATC 1 cut(s) 698
AcoI YGGCCR 1 cut(s) 117
AcyI GRCGYC 1 cut(s) 880
AdeI CACNNNGTG 1 cut(s) 356
AfaI GTAC 1 cut(s) 143
AflIII ACRYGT 1 cut(s) 138
AgsI TTSAA 7 cut(s) 59, 111, 241, 480, 719, 770, 848
AjnI CCWGG 1 cut(s) 325
AluBI AGCT 5 cut(s) 18, 93, 150, 433, 736
AluI AGCT 5 cut(s) 18, 93, 150, 433, 736
Alw21I GWGCWC 3 cut(s) 172, 304, 688
Alw26I GTCTC 2 cut(s) 540, 858
AlwI GGATC 1 cut(s) 698
AoxI GGCC 1 cut(s) 117
ApeKI GCWGC 1 cut(s) 202
AseI ATTAAT 1 cut(s) 453
Asp700I GAANNNNTTC 2 cut(s) 363, 488
BauI CACGAG 1 cut(s) 354
Bbv12I GWGCWC 3 cut(s) 172, 304, 688
BbvI GCAGC 1 cut(s) 189
BccI CCATC 2 cut(s) 375, 870
BciT130I CCWGG 1 cut(s) 327
BcoDI GTCTC 2 cut(s) 540, 858
BfaI CTAG 1 cut(s) 390
BfuAI ACCTGC 1 cut(s) 286
BglII AGATCT 1 cut(s) 712
BisI GCNGC 1 cut(s) 203
BlsI GCNGC 1 cut(s) 204
Bme1390I CCNGG 1 cut(s) 327
BmiI GGNNCC 1 cut(s) 657
BmrFI CCNGG 1 cut(s) 327
BmsI GCATC 1 cut(s) 71
BplI GAGNNNNNCTC 1 cut(s) 883
BpmI CTGGAG 3 cut(s) 421, 564, 822
BpuEI CTTGAG 3 cut(s) 70, 130, 764
BsaHI GRCGYC 1 cut(s) 880
BsaJI CCNNGG 2 cut(s) 176, 325
BsaXI ACNNNNNCTCC 2 cut(s) 569, 599
Bse1I ACTGG 2 cut(s) 106, 547
Bse3DI GCAATG 2 cut(s) 169, 219
BseBI CCWGG 1 cut(s) 327
BseDI CCNNGG 2 cut(s) 176, 325
BseGI GGATG 2 cut(s) 86, 862
BseMI GCAATG 2 cut(s) 169, 219
BseNI ACTGG 2 cut(s) 106, 547
BseXI GCAGC 1 cut(s) 189
BshFI GGCC 1 cut(s) 119
BsiHKAI GWGCWC 3 cut(s) 172, 304, 688
BsiSI CCGG 1 cut(s) 133
BsmAI GTCTC 2 cut(s) 540, 858
BsnI GGCC 1 cut(s) 119
Bsp1286I GDGCHC 3 cut(s) 172, 304, 688
Bsp143I GATC 2 cut(s) 703, 712
BspANI GGCC 1 cut(s) 119
BspLI GGNNCC 1 cut(s) 657
BspMI ACCTGC 1 cut(s) 286
BspPI GGATC 1 cut(s) 698
BsrDI GCAATG 2 cut(s) 169, 219
BsrI ACTGG 2 cut(s) 106, 547
BssECI CCNNGG 2 cut(s) 176, 325
BssMI GATC 2 cut(s) 703, 712
BssNAI GTATAC 1 cut(s) 514
BssNI GRCGYC 1 cut(s) 880
BssSI CACGAG 1 cut(s) 354
BssT1I CCWWGG 1 cut(s) 176
Bst1107I GTATAC 1 cut(s) 514
Bst2BI CACGAG 1 cut(s) 354
Bst2UI CCWGG 1 cut(s) 327
Bst6I CTCTTC 1 cut(s) 863
BstACI GRCGYC 1 cut(s) 880
BstC8I GCNNGC 2 cut(s) 45, 684
BstDEI CTNAG 2 cut(s) 305, 813
BstF5I GGATG 2 cut(s) 86, 862
BstKTI GATC 2 cut(s) 706, 715
BstMAI GTCTC 2 cut(s) 540, 858
BstMBI GATC 2 cut(s) 703, 712
BstMWI GCNNNNNNNGC 1 cut(s) 90
BstNI CCWGG 1 cut(s) 327
BstNSI RCATGY 1 cut(s) 142
BstSCI CCNGG 1 cut(s) 325
BstV1I GCAGC 1 cut(s) 189
BstX2I RGATCY 1 cut(s) 712
BstYI RGATCY 1 cut(s) 712
BstZ17I GTATAC 1 cut(s) 514
BsuRI GGCC 1 cut(s) 119
BtsCI GGATG 2 cut(s) 86, 862
BveI ACCTGC 1 cut(s) 286
Cac8I GCNNGC 2 cut(s) 45, 684
CseI GACGC 2 cut(s) 208, 869
Csp6I GTAC 1 cut(s) 142
CviAII CATG 2 cut(s) 139, 316
CviQI GTAC 1 cut(s) 142
DdeI CTNAG 2 cut(s) 305, 813
DpnI GATC 2 cut(s) 705, 714
DpnII GATC 2 cut(s) 703, 712
DraIII CACNNNGTG 1 cut(s) 356
EaeI YGGCCR 1 cut(s) 117
Eam1104I CTCTTC 1 cut(s) 863
EarI CTCTTC 1 cut(s) 863
Eco130I CCWWGG 1 cut(s) 176
Eco32I GATATC 1 cut(s) 835
EcoRII CCWGG 1 cut(s) 325
EcoRV GATATC 1 cut(s) 835
EcoT14I CCWWGG 1 cut(s) 176
ErhI CCWWGG 1 cut(s) 176
FaeI CATG 2 cut(s) 142, 319
FalI AAGNNNNNCTT 2 cut(s) 842, 874
FatI CATG 2 cut(s) 138, 315
FauNDI CATATG 3 cut(s) 445, 626, 775
FblI GTMKAC 1 cut(s) 513
Fnu4HI GCNGC 1 cut(s) 203
FokI GGATG 2 cut(s) 93, 849
Fsp4HI GCNGC 1 cut(s) 203
FspBI CTAG 1 cut(s) 390
GluI GCNGC 1 cut(s) 203
GsuI CTGGAG 3 cut(s) 421, 564, 822
HaeIII GGCC 1 cut(s) 119
HapII CCGG 1 cut(s) 133
HgaI GACGC 2 cut(s) 208, 869
Hin1I GRCGYC 1 cut(s) 880
Hin1II CATG 2 cut(s) 142, 319
HindIII AAGCTT 1 cut(s) 148
HinfI GANTC 6 cut(s) 28, 55, 62, 484, 620, 779
HpaII CCGG 1 cut(s) 133
Hpy166II GTNNAC 2 cut(s) 387, 514
Hpy188I TCNGA 2 cut(s) 14, 592
Hpy188III TCNNGA 6 cut(s) 59, 87, 238, 356, 376, 716
Hpy8I GTNNAC 2 cut(s) 387, 514
HpyCH4V TGCA 3 cut(s) 212, 350, 443
HpyF10VI GCNNNNNNNGC 1 cut(s) 90
HpyF3I CTNAG 2 cut(s) 305, 813
Hsp92I GRCGYC 1 cut(s) 880
Hsp92II CATG 2 cut(s) 142, 319
Kzo9I GATC 2 cut(s) 703, 712
LmnI GCTCC 3 cut(s) 167, 299, 655
Lsp1109I GCAGC 1 cut(s) 189
LweI GCATC 1 cut(s) 71
MaeI CTAG 1 cut(s) 390
MaeIII GTNAC 1 cut(s) 571
MalI GATC 2 cut(s) 705, 714
MboI GATC 2 cut(s) 703, 712
MboII GAAGA 6 cut(s) 57, 65, 226, 417, 428, 850
MfeI CAATTG 1 cut(s) 660
MflI RGATCY 1 cut(s) 712
MhlI GDGCHC 3 cut(s) 172, 304, 688
MluCI AATT 3 cut(s) 423, 458, 660
MlyI GAGTC 1 cut(s) 773
MnlI CCTC 3 cut(s) 363, 811, 885
MroXI GAANNNNTTC 2 cut(s) 363, 488
MseI TTAA 5 cut(s) 126, 435, 453, 461, 560
MspI CCGG 1 cut(s) 133
MspR9I CCNGG 1 cut(s) 327
MunI CAATTG 1 cut(s) 660
MvaI CCWGG 1 cut(s) 327
MwoI GCNNNNNNNGC 1 cut(s) 90
NdeI CATATG 3 cut(s) 445, 626, 775
NdeII GATC 2 cut(s) 703, 712
NlaIII CATG 2 cut(s) 142, 319
NlaIV GGNNCC 1 cut(s) 657
NspI RCATGY 1 cut(s) 142
PaqCI CACCTGC 1 cut(s) 286
PciI ACATGT 1 cut(s) 138
PcsI WCGNNNNNNNCGW 1 cut(s) 679
PdmI GAANNNNTTC 2 cut(s) 363, 488
PfeI GAWTC 5 cut(s) 28, 55, 62, 484, 620
PkrI GCNGC 1 cut(s) 204
PleI GAGTC 1 cut(s) 773
PpsI GAGTC 1 cut(s) 773
PscI ACATGT 1 cut(s) 138
PshBI ATTAAT 1 cut(s) 453
Psp6I CCWGG 1 cut(s) 325
PspGI CCWGG 1 cut(s) 325
PspN4I GGNNCC 1 cut(s) 657
PsuI RGATCY 1 cut(s) 712
RsaI GTAC 1 cut(s) 143
RsaNI GTAC 1 cut(s) 142
SaqAI TTAA 5 cut(s) 126, 435, 453, 461, 560
SatI GCNGC 1 cut(s) 203
Sau3AI GATC 2 cut(s) 703, 712
SchI GAGTC 1 cut(s) 773
ScrFI CCNGG 1 cut(s) 327
SduI GDGCHC 3 cut(s) 172, 304, 688
SfaNI GCATC 1 cut(s) 71
SmlI CTYRAG 3 cut(s) 85, 145, 743
SmoI CTYRAG 3 cut(s) 85, 145, 743
Sse9I AATT 3 cut(s) 423, 458, 660
SspMI CTAG 1 cut(s) 390
StyD4I CCNGG 1 cut(s) 325
StyI CCWWGG 1 cut(s) 176
TaqI TCGA 1 cut(s) 222
TasI AATT 3 cut(s) 423, 458, 660
TatI WGTACW 1 cut(s) 141
TfiI GAWTC 5 cut(s) 28, 55, 62, 484, 620
Tru1I TTAA 5 cut(s) 126, 435, 453, 461, 560
Tru9I TTAA 5 cut(s) 126, 435, 453, 461, 560
TseI GCWGC 1 cut(s) 202
TspDTI ATGAA 1 cut(s) 618
TspGWI ACGGA 3 cut(s) 111, 590, 818
VspI ATTAAT 1 cut(s) 453
XceI RCATGY 1 cut(s) 142
XmiI GTMKAC 1 cut(s) 513
XmnI GAANNNNTTC 2 cut(s) 363, 488
XspI CTAG 1 cut(s) 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.