RLG00000025488

39S ribosomal protein L45

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0013393
Physical Location & Seq
Forward (+)
46044073 .. 46047987
3915 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025488

Sequence Viewer

Length: 870 bp
ATGGCTTTCAGAAGCTCAAACACGATTCGTGTTCTCTTGCGTGCGACCCAGATTCAAGAATCTTCGTATCTGCTTGGATTGGCCGAACTTAATAGCCGGAACATGTACTCAAGCTTATATAAGGGCAATGGAGCACTCCCTTGGACTTCTAGGAATGCTATGACGCTGCGTTCTGCAATGGCTGTTGAGTTGCCAATCTTCTTGAATGATGAAAGGTTGCTATCCACACAAGTAAAAGCACCTGCCCAAGCAAGACAAATGGGAGCACTTAGAGTTTCCATGACAAGCCCAGGATTTATCTATGAGCCTTATGCACCTCGTGAAAAAGTTCCATTTTTCCGGAGATGGTTCACTAGAAGTGGCTGGAGAAGAACAAAAGAAGATATAATTTTAGAGCTTAAAAGTGCATATGCGATTAATAAATTAAGAAAATCGGGATATTCAAAGAATTTATTCTACAAAGAAGCAATCAATGTATACAAGGAGATAAATACTCTATTGGCAACTGGAGACAAAAACACTTTAAGGAAAGCAGTTACCGAGAAAATGTACTCTGAACTAAAGAATGAAATAAAACAGAGAGAATCCATATGGAGTAAGGTTTATTGGGAAATGGTGGAGCCAATTGTAAAAATACGCACCTTGCGAGCACGACTGATTGGTGTTGATCCAAAAGATCTCGAAAAGGTGTTTATACAGCTTACGCTTGAGTTTCTGTCAAAGCAGAAGTTCGAAGCATATGACTCAAAGGGTTCTGTTGTTGCTGGAGATAAATCTAAGGAGGTTCTTGTCCGTGATATCTGGGTATTTGAAAAGTCTCTCTTCCATCCTAAATCTTTCTGGCGTCTTTGTGGGAGGATTACTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tim44

Protein Analysis

290

Amino Acids

33.59

Weight (kDa)

9.99

Isoelectric Point (pI)

42.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim44 PF04280 135 - 285 1.9e-22 Tim44-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013393)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G27395 AT5G27395
fragaria_vesca FvH4_6g05290
malus_domestica MD04G1209800.v1.1 MD12G1224400.v1.1
prunus_persica Prupe.6G330700_v2.0.a1 Prupe.6G330700_v2.0.a1
pyrus_communis pycom04g18600 pycom12g21000
rosa_chinensis RchiOBHm_Chr3g0454031
rosa_laevigata RLG00000025488
rosa_multiflora Rmu_sc0019265.1_g000003 Rmu_sc0020385.1_g000006
rosa_rugosa Rorug02G0652400
rosa_samantha Rh3AG055100 Rh3BG056700 Rh3DG057200
rosa_wichuraiana Rw3G004230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 250
Acc36I ACCTGC 1 cut(s) 250
AccI GTMKAC 1 cut(s) 477
AccIII TCCGGA 1 cut(s) 339
AclWI GGATC 1 cut(s) 662
AcoI YGGCCR 1 cut(s) 81
AcsI RAATTY 1 cut(s) 448
AcyI GRCGYC 1 cut(s) 844
AdeI CACNNNGTG 1 cut(s) 320
AfaI GTAC 2 cut(s) 107, 551
AflIII ACRYGT 1 cut(s) 102
AgsI TTSAA 4 cut(s) 56, 205, 444, 812
AjnI CCWGG 1 cut(s) 289
AluBI AGCT 4 cut(s) 15, 114, 397, 700
AluI AGCT 4 cut(s) 15, 114, 397, 700
Alw21I GWGCWC 3 cut(s) 136, 268, 652
Alw26I GTCTC 2 cut(s) 504, 822
AlwI GGATC 1 cut(s) 662
Aor13HI TCCGGA 1 cut(s) 339
AoxI GGCC 1 cut(s) 81
ApeKI GCWGC 1 cut(s) 166
ApoI RAATTY 1 cut(s) 448
AseI ATTAAT 1 cut(s) 417
Asp700I GAANNNNTTC 2 cut(s) 327, 452
AsuII TTCGAA 1 cut(s) 732
BauI CACGAG 1 cut(s) 318
Bbv12I GWGCWC 3 cut(s) 136, 268, 652
BbvI GCAGC 1 cut(s) 153
BccI CCATC 2 cut(s) 339, 834
BciT130I CCWGG 1 cut(s) 291
BcoDI GTCTC 2 cut(s) 504, 822
BfaI CTAG 2 cut(s) 150, 354
BfuAI ACCTGC 1 cut(s) 250
BglII AGATCT 1 cut(s) 676
BisI GCNGC 1 cut(s) 167
BlsI GCNGC 1 cut(s) 168
Bme1390I CCNGG 1 cut(s) 291
BmiI GGNNCC 1 cut(s) 621
BmrFI CCNGG 1 cut(s) 291
BplI GAGNNNNNCTC 1 cut(s) 847
BpmI CTGGAG 3 cut(s) 385, 528, 786
Bpu14I TTCGAA 1 cut(s) 732
BpuEI CTTGAG 2 cut(s) 94, 728
BsaHI GRCGYC 1 cut(s) 844
BsaJI CCNNGG 2 cut(s) 140, 289
BsaWI WCCGGW 1 cut(s) 339
Bse1I ACTGG 1 cut(s) 511
Bse3DI GCAATG 2 cut(s) 133, 183
BseAI TCCGGA 1 cut(s) 339
BseBI CCWGG 1 cut(s) 291
BseDI CCNNGG 2 cut(s) 140, 289
BseGI GGATG 1 cut(s) 826
BseMI GCAATG 2 cut(s) 133, 183
BseNI ACTGG 1 cut(s) 511
BseXI GCAGC 1 cut(s) 153
BshFI GGCC 1 cut(s) 83
BsiHKAI GWGCWC 3 cut(s) 136, 268, 652
BsiSI CCGG 2 cut(s) 97, 340
BsmAI GTCTC 2 cut(s) 504, 822
BsmI GAATGC 1 cut(s) 160
BsnI GGCC 1 cut(s) 83
Bsp119I TTCGAA 1 cut(s) 732
Bsp1286I GDGCHC 3 cut(s) 136, 268, 652
Bsp13I TCCGGA 1 cut(s) 339
Bsp143I GATC 2 cut(s) 667, 676
BspANI GGCC 1 cut(s) 83
BspEI TCCGGA 1 cut(s) 339
BspLI GGNNCC 1 cut(s) 621
BspMI ACCTGC 1 cut(s) 250
BspPI GGATC 1 cut(s) 662
BspT104I TTCGAA 1 cut(s) 732
BsrDI GCAATG 2 cut(s) 133, 183
BsrI ACTGG 1 cut(s) 511
BssECI CCNNGG 2 cut(s) 140, 289
BssMI GATC 2 cut(s) 667, 676
BssNAI GTATAC 1 cut(s) 478
BssNI GRCGYC 1 cut(s) 844
BssSI CACGAG 1 cut(s) 318
BssT1I CCWWGG 1 cut(s) 140
Bst1107I GTATAC 1 cut(s) 478
Bst2BI CACGAG 1 cut(s) 318
Bst2UI CCWGG 1 cut(s) 291
Bst6I CTCTTC 1 cut(s) 827
BstACI GRCGYC 1 cut(s) 844
BstBI TTCGAA 1 cut(s) 732
BstC8I GCNNGC 2 cut(s) 42, 648
BstDEI CTNAG 2 cut(s) 269, 777
BstF5I GGATG 1 cut(s) 826
BstKTI GATC 2 cut(s) 670, 679
BstMAI GTCTC 2 cut(s) 504, 822
BstMBI GATC 2 cut(s) 667, 676
BstNI CCWGG 1 cut(s) 291
BstNSI RCATGY 1 cut(s) 106
BstSCI CCNGG 1 cut(s) 289
BstV1I GCAGC 1 cut(s) 153
BstX2I RGATCY 1 cut(s) 676
BstYI RGATCY 1 cut(s) 676
BstZ17I GTATAC 1 cut(s) 478
BsuRI GGCC 1 cut(s) 83
BtsCI GGATG 1 cut(s) 826
BveI ACCTGC 1 cut(s) 250
Cac8I GCNNGC 2 cut(s) 42, 648
CseI GACGC 2 cut(s) 172, 833
Csp6I GTAC 2 cut(s) 106, 550
CviAII CATG 2 cut(s) 103, 280
CviQI GTAC 2 cut(s) 106, 550
DdeI CTNAG 2 cut(s) 269, 777
DpnI GATC 2 cut(s) 669, 678
DpnII GATC 2 cut(s) 667, 676
DraIII CACNNNGTG 1 cut(s) 320
EaeI YGGCCR 1 cut(s) 81
Eam1104I CTCTTC 1 cut(s) 827
EarI CTCTTC 1 cut(s) 827
Eco130I CCWWGG 1 cut(s) 140
Eco32I GATATC 1 cut(s) 799
EcoRII CCWGG 1 cut(s) 289
EcoRV GATATC 1 cut(s) 799
EcoT14I CCWWGG 1 cut(s) 140
ErhI CCWWGG 1 cut(s) 140
FaeI CATG 2 cut(s) 106, 283
FalI AAGNNNNNCTT 2 cut(s) 806, 838
FatI CATG 2 cut(s) 102, 279
FauNDI CATATG 3 cut(s) 409, 590, 739
FblI GTMKAC 1 cut(s) 477
Fnu4HI GCNGC 1 cut(s) 167
FokI GGATG 1 cut(s) 813
Fsp4HI GCNGC 1 cut(s) 167
FspBI CTAG 2 cut(s) 150, 354
GluI GCNGC 1 cut(s) 167
GsuI CTGGAG 3 cut(s) 385, 528, 786
HaeIII GGCC 1 cut(s) 83
HapII CCGG 2 cut(s) 97, 340
HgaI GACGC 2 cut(s) 172, 833
Hin1I GRCGYC 1 cut(s) 844
Hin1II CATG 2 cut(s) 106, 283
HindIII AAGCTT 1 cut(s) 112
HinfI GANTC 5 cut(s) 25, 52, 59, 584, 743
HpaII CCGG 2 cut(s) 97, 340
Hpy166II GTNNAC 2 cut(s) 351, 478
Hpy188I TCNGA 2 cut(s) 11, 556
Hpy188III TCNNGA 6 cut(s) 56, 202, 320, 340, 435, 680
Hpy8I GTNNAC 2 cut(s) 351, 478
HpyCH4V TGCA 3 cut(s) 176, 314, 407
HpyF3I CTNAG 2 cut(s) 269, 777
Hsp92I GRCGYC 1 cut(s) 844
Hsp92II CATG 2 cut(s) 106, 283
Kpn2I TCCGGA 1 cut(s) 339
Kzo9I GATC 2 cut(s) 667, 676
LmnI GCTCC 3 cut(s) 131, 263, 619
Lsp1109I GCAGC 1 cut(s) 153
MaeI CTAG 2 cut(s) 150, 354
MaeIII GTNAC 1 cut(s) 535
MalI GATC 2 cut(s) 669, 678
MboI GATC 2 cut(s) 667, 676
MboII GAAGA 5 cut(s) 54, 190, 381, 392, 814
MfeI CAATTG 1 cut(s) 624
MflI RGATCY 1 cut(s) 676
MhlI GDGCHC 3 cut(s) 136, 268, 652
MluCI AATT 4 cut(s) 387, 422, 448, 624
MlyI GAGTC 1 cut(s) 737
MnlI CCTC 3 cut(s) 327, 775, 849
MroI TCCGGA 1 cut(s) 339
MroXI GAANNNNTTC 2 cut(s) 327, 452
MseI TTAA 5 cut(s) 90, 399, 417, 425, 524
MspI CCGG 2 cut(s) 97, 340
MspR9I CCNGG 1 cut(s) 291
MunI CAATTG 1 cut(s) 624
Mva1269I GAATGC 1 cut(s) 160
MvaI CCWGG 1 cut(s) 291
NdeI CATATG 3 cut(s) 409, 590, 739
NdeII GATC 2 cut(s) 667, 676
NlaIII CATG 2 cut(s) 106, 283
NlaIV GGNNCC 1 cut(s) 621
NspI RCATGY 1 cut(s) 106
NspV TTCGAA 1 cut(s) 732
PaqCI CACCTGC 1 cut(s) 250
PciI ACATGT 1 cut(s) 102
PcsI WCGNNNNNNNCGW 1 cut(s) 643
PctI GAATGC 1 cut(s) 160
PdmI GAANNNNTTC 2 cut(s) 327, 452
PfeI GAWTC 4 cut(s) 25, 52, 59, 584
PkrI GCNGC 1 cut(s) 168
PleI GAGTC 1 cut(s) 737
PpsI GAGTC 1 cut(s) 737
PscI ACATGT 1 cut(s) 102
PshBI ATTAAT 1 cut(s) 417
Psp6I CCWGG 1 cut(s) 289
PspGI CCWGG 1 cut(s) 289
PspN4I GGNNCC 1 cut(s) 621
PsuI RGATCY 1 cut(s) 676
RsaI GTAC 2 cut(s) 107, 551
RsaNI GTAC 2 cut(s) 106, 550
SaqAI TTAA 5 cut(s) 90, 399, 417, 425, 524
SatI GCNGC 1 cut(s) 167
Sau3AI GATC 2 cut(s) 667, 676
SchI GAGTC 1 cut(s) 737
ScrFI CCNGG 1 cut(s) 291
SduI GDGCHC 3 cut(s) 136, 268, 652
SfuI TTCGAA 1 cut(s) 732
SmlI CTYRAG 2 cut(s) 109, 707
SmoI CTYRAG 2 cut(s) 109, 707
Sse9I AATT 4 cut(s) 387, 422, 448, 624
SspMI CTAG 2 cut(s) 150, 354
StyD4I CCNGG 1 cut(s) 289
StyI CCWWGG 1 cut(s) 140
TaqI TCGA 2 cut(s) 681, 732
TasI AATT 4 cut(s) 387, 422, 448, 624
TatI WGTACW 2 cut(s) 105, 549
TfiI GAWTC 4 cut(s) 25, 52, 59, 584
Tru1I TTAA 5 cut(s) 90, 399, 417, 425, 524
Tru9I TTAA 5 cut(s) 90, 399, 417, 425, 524
TseI GCWGC 1 cut(s) 166
TspDTI ATGAA 2 cut(s) 225, 582
TspGWI ACGGA 1 cut(s) 782
VspI ATTAAT 1 cut(s) 417
XapI RAATTY 1 cut(s) 448
XceI RCATGY 1 cut(s) 106
XmiI GTMKAC 1 cut(s) 477
XmnI GAANNNNTTC 2 cut(s) 327, 452
XspI CTAG 2 cut(s) 150, 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.