AT5G27710

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008790
Physical Location & Seq
Forward (+)
9812931 .. 9815420
2490 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G27710.1

Sequence Viewer

Length: 1008 bp
ATGGTGAATTCGTACGTTCCTCTTCGTTTCACCATTTTCATCTCCTTCTCCATCGCAGCAGCTTCATCCTTCAAACTCCACTCCGCTTCTCATTCTCCGTCGTCATTTCCGAAAGCCACCGGCGACGATCTACTCTCCGTTCTCGGACCTCCCTCCGCCGCGTCGTGCCTCAACCCAATCGTCTCTCGAGAGATCAAATCGTGTCTTAAATTCCTCGTCCCATTCAAATCGGATAAACCTAAACCCGAATTTGGACGATGCTCGCCTCGTACTGGACTCTGTTCCGGAAAAATTGACGCCGTTGAGAGACGGAGCAAGTTTGAAGAGGAGAATTCTCTAATCTGGTGGCCACCGGAATCGGTTCTAGAGCTTGCTCGTCTCGCTGTTGACTCCGGTGGTGATCCCGGTTCAATTCAGCGAACTCTCAATCCGAAAATGATCCCGGTTCCTGATGTTGAACGATCAAGAAAAGATAAATGTCAACTTACTAGAACTCCTTATGGTCGTCATTTTATAGCAGAGGAAGTGAACTCATATTTCGAGTTCTTGTTTCATCTAATTGAATCTAGGGGACCTAGTGTTGGATTGAATGTTTCATTGAGTAGATATGATTTGTTCCATGGCCATTTATTCCTTGCCTCAGAATCTGGACGCCTTGGAATATTGTTTCATGCTAAAGAGTATCCAGCCTATGACAAGAAAGTGTTTCCTTACAACATGGGATATTGCCAGAGAGGATCTGACGTGAAATACAATGATTCAATGAACTTGAGGAATATCCTTTGGCTTGCACCATTACCTAGCAACTCTTCTCCAGATTGGGTTGCTCCAGGTGTACTTGTGGTTCTTGATGCGCATCCGGATGGAATCATATACCGAGATCTCATCCCTGACTATGTTAAGTTTGTAAGAACAATATACGAAGATGATTTGGGAACAACCGCAGTTGATGTCAATTACTTGAATGTCGGAGCTCACGAACCTGACTATCAGCTTTTCATGTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

335

Amino Acids

37.52

Weight (kDa)

6.51

Isoelectric Point (pI)

41.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 855
AccII CGCG 1 cut(s) 161
AccIII TCCGGA 2 cut(s) 284, 859
AciI CCGC 4 cut(s) 84, 156, 159, 942
AclWI GGATC 3 cut(s) 395, 433, 745
AcoI YGGCCR 2 cut(s) 347, 622
AcsI RAATTY 4 cut(s) 7, 209, 248, 331
AcyI GRCGYC 2 cut(s) 297, 652
AfaI GTAC 3 cut(s) 14, 271, 837
AfiI CCNNNNNNNGG 3 cut(s) 251, 272, 581
AgsI TTSAA 9 cut(s) 73, 226, 323, 411, 458, 563, 589, 762, 964
AjiI CACGTC 1 cut(s) 745
AjnI CCWGG 1 cut(s) 829
AloI GAACNNNNNNTCC 2 cut(s) 412, 444
AluBI AGCT 4 cut(s) 62, 370, 974, 994
AluI AGCT 4 cut(s) 62, 370, 974, 994
Alw21I GWGCWC 1 cut(s) 976
Alw26I GTCTC 3 cut(s) 187, 301, 383
AlwI GGATC 3 cut(s) 395, 433, 745
AlwNI CAGNNNCTG 1 cut(s) 647
Ama87I CYCGRG 1 cut(s) 186
Aor13HI TCCGGA 2 cut(s) 284, 859
AoxI GGCC 2 cut(s) 347, 622
ApeKI GCWGC 2 cut(s) 56, 59
ApoI RAATTY 4 cut(s) 7, 209, 248, 331
Asp700I GAANNNNTTC 1 cut(s) 360
AspLEI GCGC 1 cut(s) 856
AspS9I GGNCC 2 cut(s) 146, 572
AsuC2I CCSGG 2 cut(s) 405, 443
AsuHPI GGTGA 3 cut(s) 16, 22, 410
AvaI CYCGRG 1 cut(s) 186
AvaII GGWCC 2 cut(s) 146, 572
BalI TGGCCA 2 cut(s) 349, 624
BanII GRGCYC 1 cut(s) 976
Bbv12I GWGCWC 1 cut(s) 976
BbvI GCAGC 2 cut(s) 68, 71
BccI CCATC 2 cut(s) 59, 857
BceAI ACGGC 1 cut(s) 284
BciT130I CCWGG 1 cut(s) 831
BciVI GTATCC 1 cut(s) 693
BcnI CCSGG 2 cut(s) 405, 443
BcoDI GTCTC 3 cut(s) 187, 301, 383
BfaI CTAG 5 cut(s) 365, 489, 567, 576, 801
BfuI GTATCC 1 cut(s) 693
BglII AGATCT 1 cut(s) 880
BisI GCNGC 3 cut(s) 57, 60, 159
BlsI GCNGC 3 cut(s) 58, 61, 160
Bme1390I CCNGG 3 cut(s) 405, 443, 831
Bme18I GGWCC 2 cut(s) 146, 572
BmeT110I CYCGRG 1 cut(s) 186
BmgBI CACGTC 1 cut(s) 745
BmgT120I GGNCC 2 cut(s) 146, 572
BmiI GGNNCC 2 cut(s) 447, 573
BmrFI CCNGG 3 cut(s) 405, 443, 831
BmsI GCATC 3 cut(s) 248, 841, 865
BpmI CTGGAG 2 cut(s) 798, 813
BpuEI CTTGAG 1 cut(s) 790
BpuMI CCSGG 2 cut(s) 405, 443
BsaBI GATNNNNATC 1 cut(s) 855
BsaHI GRCGYC 2 cut(s) 297, 652
BsaJI CCNNGG 2 cut(s) 619, 655
BsaWI WCCGGW 4 cut(s) 284, 352, 392, 859
BsaXI ACNNNNNCTCC 2 cut(s) 478, 508
Bsc4I CCNNNNNNNGG 3 cut(s) 251, 272, 581
Bse118I RCCGGY 1 cut(s) 119
Bse1I ACTGG 1 cut(s) 277
Bse8I GATNNNNATC 1 cut(s) 855
BseAI TCCGGA 2 cut(s) 284, 859
BseBI CCWGG 1 cut(s) 831
BseDI CCNNGG 2 cut(s) 619, 655
BseGI GGATG 4 cut(s) 65, 856, 868, 885
BseJI GATNNNNATC 1 cut(s) 855
BseLI CCNNNNNNNGG 3 cut(s) 251, 272, 581
BseMII CTCAG 1 cut(s) 654
BseNI ACTGG 1 cut(s) 277
BseRI GAGGAG 1 cut(s) 341
BseXI GCAGC 2 cut(s) 68, 71
Bsh1236I CGCG 1 cut(s) 161
BshFI GGCC 2 cut(s) 349, 624
BsiHKAI GWGCWC 1 cut(s) 976
BsiHKCI CYCGRG 1 cut(s) 186
BsiSI CCGG 7 cut(s) 120, 285, 353, 393, 405, 443, 860
BsiWI CGTACG 1 cut(s) 12
BslFI GGGAC 2 cut(s) 203, 585
BslI CCNNNNNNNGG 3 cut(s) 251, 272, 581
BsmAI GTCTC 3 cut(s) 187, 301, 383
BsmBI CGTCTC 3 cut(s) 187, 301, 383
BsmFI GGGAC 2 cut(s) 203, 585
BsnI GGCC 2 cut(s) 349, 624
BsoBI CYCGRG 1 cut(s) 186
Bsp1286I GDGCHC 1 cut(s) 976
Bsp13I TCCGGA 2 cut(s) 284, 859
Bsp143I GATC 7 cut(s) 127, 192, 400, 438, 461, 737, 880
Bsp19I CCATGG 1 cut(s) 619
BspACI CCGC 4 cut(s) 84, 156, 159, 942
BspANI GGCC 2 cut(s) 349, 624
BspCNI CTCAG 1 cut(s) 653
BspEI TCCGGA 2 cut(s) 284, 859
BspFNI CGCG 1 cut(s) 161
BspLI GGNNCC 2 cut(s) 447, 573
BspPI GGATC 3 cut(s) 395, 433, 745
BsrFI RCCGGY 1 cut(s) 119
BsrI ACTGG 1 cut(s) 277
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 2 cut(s) 619, 655
BssMI GATC 7 cut(s) 127, 192, 400, 438, 461, 737, 880
BssNI GRCGYC 2 cut(s) 297, 652
BssT1I CCWWGG 2 cut(s) 619, 655
Bst2UI CCWGG 1 cut(s) 831
Bst6I CTCTTC 3 cut(s) 27, 318, 814
BstACI GRCGYC 2 cut(s) 297, 652
BstC8I GCNNGC 3 cut(s) 263, 372, 789
BstDEI CTNAG 1 cut(s) 640
BstDSI CCRYGG 1 cut(s) 619
BstF5I GGATG 4 cut(s) 65, 856, 868, 885
BstFNI CGCG 1 cut(s) 161
BstHHI GCGC 1 cut(s) 856
BstKTI GATC 7 cut(s) 130, 195, 403, 441, 464, 740, 883
BstMAI GTCTC 3 cut(s) 187, 301, 383
BstMBI GATC 7 cut(s) 127, 192, 400, 438, 461, 737, 880
BstMWI GCNNNNNNNGC 1 cut(s) 380
BstNI CCWGG 1 cut(s) 831
BstSCI CCNGG 3 cut(s) 403, 441, 829
BstUI CGCG 1 cut(s) 161
BstV1I GCAGC 2 cut(s) 68, 71
BstX2I RGATCY 2 cut(s) 737, 880
BstYI RGATCY 2 cut(s) 737, 880
BsuI GTATCC 1 cut(s) 693
BsuRI GGCC 2 cut(s) 349, 624
BtgI CCRYGG 1 cut(s) 619
BtgZI GCGATG 1 cut(s) 37
BtrI CACGTC 1 cut(s) 745
BtsCI GGATG 4 cut(s) 65, 856, 868, 885
Cac8I GCNNGC 3 cut(s) 263, 372, 789
CaiI CAGNNNCTG 1 cut(s) 647
CfoI GCGC 1 cut(s) 856
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 2 cut(s) 146, 572
CseI GACGC 3 cut(s) 150, 305, 660
Csp6I GTAC 3 cut(s) 13, 270, 836
CviAII CATG 4 cut(s) 620, 671, 718, 1000
CviJI RGCY 9 cut(s) 62, 116, 349, 370, 624, 689, 787, 974, 994
CviKI_1 RGCY 9 cut(s) 62, 116, 349, 370, 624, 689, 787, 974, 994
CviQI GTAC 3 cut(s) 13, 270, 836
DdeI CTNAG 1 cut(s) 640
DpnI GATC 7 cut(s) 129, 194, 402, 440, 463, 739, 882
DpnII GATC 7 cut(s) 127, 192, 400, 438, 461, 737, 880
EaeI YGGCCR 2 cut(s) 347, 622
Eam1104I CTCTTC 3 cut(s) 27, 318, 814
EarI CTCTTC 3 cut(s) 27, 318, 814
EciI GGCGGA 1 cut(s) 145
Ecl136II GAGCTC 1 cut(s) 974
Eco130I CCWWGG 2 cut(s) 619, 655
Eco24I GRGCYC 1 cut(s) 976
Eco47I GGWCC 2 cut(s) 146, 572
Eco53kI GAGCTC 1 cut(s) 974
Eco88I CYCGRG 1 cut(s) 186
EcoICRI GAGCTC 1 cut(s) 974
EcoO109I RGGNCCY 1 cut(s) 572
EcoRI GAATTC 2 cut(s) 7, 331
EcoRII CCWGG 1 cut(s) 829
EcoT14I CCWWGG 2 cut(s) 619, 655
EcoT38I GRGCYC 1 cut(s) 976
ErhI CCWWGG 2 cut(s) 619, 655
Esp3I CGTCTC 3 cut(s) 187, 301, 383
FaeI CATG 4 cut(s) 623, 674, 721, 1003
FaqI GGGAC 2 cut(s) 203, 585
FatI CATG 4 cut(s) 619, 670, 717, 999
Fnu4HI GCNGC 3 cut(s) 57, 60, 159
FokI GGATG 4 cut(s) 52, 843, 872, 875
FriOI GRGCYC 1 cut(s) 976
Fsp4HI GCNGC 3 cut(s) 57, 60, 159
FspAI RTGCGCAY 1 cut(s) 855
FspBI CTAG 5 cut(s) 365, 489, 567, 576, 801
FspI TGCGCA 1 cut(s) 855
GlaI GCGC 1 cut(s) 855
GluI GCNGC 3 cut(s) 57, 60, 159
GsuI CTGGAG 2 cut(s) 798, 813
HaeIII GGCC 2 cut(s) 349, 624
HapII CCGG 7 cut(s) 120, 285, 353, 393, 405, 443, 860
HgaI GACGC 3 cut(s) 150, 305, 660
HhaI GCGC 1 cut(s) 856
Hin1I GRCGYC 2 cut(s) 297, 652
Hin1II CATG 4 cut(s) 623, 674, 721, 1003
Hin6I GCGC 1 cut(s) 854
HinP1I GCGC 1 cut(s) 854
HincII GTYRAC 2 cut(s) 388, 482
HindII GTYRAC 2 cut(s) 388, 482
HinfI GANTC 7 cut(s) 276, 356, 389, 563, 644, 758, 867
HpaII CCGG 7 cut(s) 120, 285, 353, 393, 405, 443, 860
HphI GGTGA 3 cut(s) 16, 22, 410
Hpy166II GTNNAC 4 cut(s) 388, 482, 529, 836
Hpy188I TCNGA 7 cut(s) 111, 146, 232, 432, 643, 742, 971
Hpy8I GTNNAC 4 cut(s) 388, 482, 529, 836
Hpy99I CGWCG 3 cut(s) 103, 128, 166
HpyAV CCTTC 2 cut(s) 55, 79
HpyCH4IV ACGT 2 cut(s) 15, 744
HpyCH4V TGCA 1 cut(s) 791
HpyF10VI GCNNNNNNNGC 1 cut(s) 380
HpyF3I CTNAG 1 cut(s) 640
HpySE526I ACGT 2 cut(s) 15, 744
Hsp92I GRCGYC 2 cut(s) 297, 652
Hsp92II CATG 4 cut(s) 623, 674, 721, 1003
HspAI GCGC 1 cut(s) 854
Kpn2I TCCGGA 2 cut(s) 284, 859
Kzo9I GATC 7 cut(s) 127, 192, 400, 438, 461, 737, 880
LmnI GCTCC 3 cut(s) 312, 832, 971
Lsp1109I GCAGC 2 cut(s) 68, 71
LweI GCATC 3 cut(s) 248, 841, 865
MaeI CTAG 5 cut(s) 365, 489, 567, 576, 801
MaeII ACGT 2 cut(s) 15, 744
MalI GATC 7 cut(s) 129, 194, 402, 440, 463, 739, 882
MboI GATC 7 cut(s) 127, 192, 400, 438, 461, 737, 880
MboII GAAGA 4 cut(s) 14, 335, 801, 935
MflI RGATCY 2 cut(s) 737, 880
MhlI GDGCHC 1 cut(s) 976
MlsI TGGCCA 2 cut(s) 349, 624
MluCI AATT 8 cut(s) 7, 209, 248, 291, 331, 411, 558, 955
MluNI TGGCCA 2 cut(s) 349, 624
MlyI GAGTC 2 cut(s) 270, 383
MmeI TCCRAC 2 cut(s) 562, 949
Mox20I TGGCCA 2 cut(s) 349, 624
MroI TCCGGA 2 cut(s) 284, 859
MroXI GAANNNNTTC 1 cut(s) 360
MscI TGGCCA 2 cut(s) 349, 624
MseI TTAA 2 cut(s) 207, 900
MslI CAYNNNNRTG 1 cut(s) 861
Msp20I TGGCCA 2 cut(s) 349, 624
MspI CCGG 7 cut(s) 120, 285, 353, 393, 405, 443, 860
MspR9I CCNGG 3 cut(s) 405, 443, 831
MvaI CCWGG 1 cut(s) 831
MvnI CGCG 1 cut(s) 161
MwoI GCNNNNNNNGC 1 cut(s) 380
NciI CCSGG 2 cut(s) 405, 443
NcoI CCATGG 1 cut(s) 619
NdeII GATC 7 cut(s) 127, 192, 400, 438, 461, 737, 880
NlaIII CATG 4 cut(s) 623, 674, 721, 1003
NlaIV GGNNCC 2 cut(s) 447, 573
NsbI TGCGCA 1 cut(s) 855
PaeR7I CTCGAG 1 cut(s) 186
PcsI WCGNNNNNNNCGW 2 cut(s) 107, 975
PdmI GAANNNNTTC 1 cut(s) 360
PfeI GAWTC 5 cut(s) 356, 563, 644, 758, 867
Pfl23II CGTACG 1 cut(s) 12
PkrI GCNGC 3 cut(s) 58, 61, 160
PleI GAGTC 2 cut(s) 270, 383
PpsI GAGTC 2 cut(s) 270, 383
PpuMI RGGWCCY 1 cut(s) 572
Psp124BI GAGCTC 1 cut(s) 976
Psp5II RGGWCCY 1 cut(s) 572
Psp6I CCWGG 1 cut(s) 829
PspGI CCWGG 1 cut(s) 829
PspLI CGTACG 1 cut(s) 12
PspN4I GGNNCC 2 cut(s) 447, 573
PspPI GGNCC 2 cut(s) 146, 572
PspPPI RGGWCCY 1 cut(s) 572
PsrI GAACNNNNNNTAC 2 cut(s) 828, 860
PstNI CAGNNNCTG 1 cut(s) 647
PsuI RGATCY 2 cut(s) 737, 880
RsaI GTAC 3 cut(s) 14, 271, 837
RsaNI GTAC 3 cut(s) 13, 270, 836
RseI CAYNNNNRTG 1 cut(s) 861
SacI GAGCTC 1 cut(s) 976
SaqAI TTAA 2 cut(s) 207, 900
SatI GCNGC 3 cut(s) 57, 60, 159
Sau3AI GATC 7 cut(s) 127, 192, 400, 438, 461, 737, 880
Sau96I GGNCC 2 cut(s) 146, 572
SchI GAGTC 2 cut(s) 270, 383
ScrFI CCNGG 3 cut(s) 405, 443, 831
SduI GDGCHC 1 cut(s) 976
SfaNI GCATC 3 cut(s) 248, 841, 865
Sfr274I CTCGAG 1 cut(s) 186
SgrAI CRCCGGYG 1 cut(s) 119
SinI GGWCC 2 cut(s) 146, 572
SlaI CTCGAG 1 cut(s) 186
SmiMI CAYNNNNRTG 1 cut(s) 861
SmlI CTYRAG 2 cut(s) 186, 769
SmoI CTYRAG 2 cut(s) 186, 769
Sse9I AATT 8 cut(s) 7, 209, 248, 291, 331, 411, 558, 955
SsiI CCGC 4 cut(s) 84, 156, 159, 942
SspI AATATT 1 cut(s) 663
SspMI CTAG 5 cut(s) 365, 489, 567, 576, 801
SstI GAGCTC 1 cut(s) 976
StyD4I CCNGG 3 cut(s) 403, 441, 829
StyI CCWWGG 2 cut(s) 619, 655
TaiI ACGT 2 cut(s) 18, 747
TaqI TCGA 2 cut(s) 187, 540
TasI AATT 8 cut(s) 7, 209, 248, 291, 331, 411, 558, 955
TatI WGTACW 1 cut(s) 835
TauI GCSGC 1 cut(s) 161
TfiI GAWTC 5 cut(s) 356, 563, 644, 758, 867
Tru1I TTAA 2 cut(s) 207, 900
Tru9I TTAA 2 cut(s) 207, 900
TseI GCWGC 2 cut(s) 56, 59
TspDTI ATGAA 7 cut(s) 28, 54, 542, 585, 659, 779, 988
TspGWI ACGGA 3 cut(s) 87, 127, 325
VpaK11BI GGWCC 2 cut(s) 146, 572
XapI RAATTY 4 cut(s) 7, 209, 248, 331
XbaI TCTAGA 1 cut(s) 364
XhoI CTCGAG 1 cut(s) 186
XmnI GAANNNNTTC 1 cut(s) 360
XspI CTAG 5 cut(s) 365, 489, 567, 576, 801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.