AT5G27860

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0015934
Physical Location & Seq
Reverse (-)
9874718 .. 9876500
1783 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G27860.1

Sequence Viewer

Length: 534 bp
ATGGCGGCTTCATCATCTTCGTCTTCCTCCGACGTCTCCTCTGATTCTTCAGACTCTCATCGTCGCCGTAAGGATCGCCGTCATCATCGTAGAAACGATCGAGACAGAGACTCGCTCAAAGTACGGAAAAAGAGCCGATCCACCTCGAAAAAACGCCGTAGACGCCAACACTCCTCCGATTCCTCTGATTCTTCTTACTCCGATTCCTCCAGTGAGAGCTCAGATAGTGAGCATGAGAAGTCACGAAGGCACAAGAAGCATGAAAAGCCAAAGAAGGCTAAGGATAAGGAACGAAGCAAGAGTCATCGTCATAAACGGCATAAGAACAGAGAAAGAAAGAAAGGGGAAGGAGAAGGAAGTAGCGGGCCTGTAAAACTATCAAAGTTTTTGAACCGCGACAAAGACGATGGTGAACGTAGAAGTGCTGTTTCAGGGAAAAAAATTCTGCTGAAGGTTGACAAGTCCAAAGAGGACAAAGCTGCAGAGAGCAAAAGAAATGAACTGCTTAAGTTCTTAAACGCAAGTTTCGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

177

Amino Acids

20.48

Weight (kDa)

10.54

Isoelectric Point (pI)

87.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015934)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G27860 AT5G27860
fragaria_vesca FvH4_2g14440
malus_domestica MD10G1017700.v1.1
prunus_persica Prupe.8G021600_v2.0.a1
pyrus_communis pycom10g01140
rosa_chinensis RchiOBHm_Chr6g0276771
rosa_laevigata RLG00000013363
rosa_multiflora Rmu_sc0004481.1_g000016
rosa_roxburghii Rroxscaffold_7G00191980
rosa_rugosa Rorug06G0104100
rosa_samantha Rh6AG214200 Rh6BG219000 Rh6CG221700
rosa_wichuraiana Rw6G018730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 36
AccI GTMKAC 1 cut(s) 160
AccII CGCG 1 cut(s) 396
AciI CCGC 3 cut(s) 5, 363, 394
AclWI GGATC 2 cut(s) 81, 132
AcsI RAATTY 1 cut(s) 441
AcuI CTGAAG 2 cut(s) 33, 470
AcyI GRCGYC 2 cut(s) 33, 163
AfaI GTAC 1 cut(s) 123
AflII CTTAAG 1 cut(s) 506
AgsI TTSAA 1 cut(s) 391
AluBI AGCT 2 cut(s) 219, 479
AluI AGCT 2 cut(s) 219, 479
Alw21I GWGCWC 1 cut(s) 221
Alw26I GTCTC 3 cut(s) 40, 96, 102
AlwI GGATC 2 cut(s) 81, 132
AoxI GGCC 1 cut(s) 365
ApeKI GCWGC 1 cut(s) 479
ApoI RAATTY 1 cut(s) 441
ArsI GACNNNNNNTTYG 2 cut(s) 286, 318
AspS9I GGNCC 1 cut(s) 365
AsuHPI GGTGA 1 cut(s) 422
BanII GRGCYC 1 cut(s) 221
BbsI GAAGAC 1 cut(s) 15
Bbv12I GWGCWC 1 cut(s) 221
BbvI GCAGC 1 cut(s) 466
BccI CCATC 1 cut(s) 401
BceAI ACGGC 4 cut(s) 51, 63, 141, 332
BcoDI GTCTC 3 cut(s) 40, 96, 102
BfaI CTAG 1 cut(s) 532
BfmI CTRYAG 1 cut(s) 480
BfrI CTTAAG 1 cut(s) 506
BisI GCNGC 2 cut(s) 6, 480
BlsI GCNGC 2 cut(s) 7, 481
BmgT120I GGNCC 1 cut(s) 365
BpiI GAAGAC 1 cut(s) 15
BplI GAGNNNNNCTC 2 cut(s) 99, 131
BpmI CTGGAG 1 cut(s) 193
Bpu10I CCTNAGC 1 cut(s) 279
BsaHI GRCGYC 2 cut(s) 33, 163
Bse1I ACTGG 1 cut(s) 210
BseMII CTCAG 1 cut(s) 234
BseNI ACTGG 1 cut(s) 210
BseRI GAGGAG 2 cut(s) 28, 163
BseXI GCAGC 1 cut(s) 466
Bsh1236I CGCG 1 cut(s) 396
Bsh1285I CGRYCG 1 cut(s) 100
BshFI GGCC 1 cut(s) 367
BsiEI CGRYCG 1 cut(s) 100
BsiHKAI GWGCWC 1 cut(s) 221
BsmAI GTCTC 3 cut(s) 40, 96, 102
BsmBI CGTCTC 1 cut(s) 40
BsnI GGCC 1 cut(s) 367
Bsp1286I GDGCHC 1 cut(s) 221
Bsp143I GATC 3 cut(s) 73, 97, 137
BspACI CCGC 3 cut(s) 5, 363, 394
BspANI GGCC 1 cut(s) 367
BspCNI CTCAG 1 cut(s) 233
BspFNI CGCG 1 cut(s) 396
BspMAI CTGCAG 1 cut(s) 484
BspPI GGATC 2 cut(s) 81, 132
BspTI CTTAAG 1 cut(s) 506
BsrI ACTGG 1 cut(s) 210
BssMI GATC 3 cut(s) 73, 97, 137
BssNI GRCGYC 2 cut(s) 33, 163
BstACI GRCGYC 2 cut(s) 33, 163
BstAFI CTTAAG 1 cut(s) 506
BstC8I GCNNGC 1 cut(s) 365
BstDEI CTNAG 2 cut(s) 220, 279
BstFNI CGCG 1 cut(s) 396
BstKTI GATC 3 cut(s) 76, 100, 140
BstMAI GTCTC 3 cut(s) 40, 96, 102
BstMBI GATC 3 cut(s) 73, 97, 137
BstMCI CGRYCG 1 cut(s) 100
BstMWI GCNNNNNNNGC 3 cut(s) 162, 256, 265
BstSFI CTRYAG 1 cut(s) 480
BstUI CGCG 1 cut(s) 396
BstV1I GCAGC 1 cut(s) 466
BstV2I GAAGAC 1 cut(s) 15
BsuRI GGCC 1 cut(s) 367
BtsIMutI CAGTG 1 cut(s) 217
Cac8I GCNNGC 1 cut(s) 365
Cfr13I GGNCC 1 cut(s) 365
CseI GACGC 1 cut(s) 171
Csp6I GTAC 1 cut(s) 122
CviAII CATG 2 cut(s) 233, 260
CviJI RGCY 7 cut(s) 8, 135, 219, 268, 278, 367, 479
CviKI_1 RGCY 7 cut(s) 8, 135, 219, 268, 278, 367, 479
CviQI GTAC 1 cut(s) 122
DdeI CTNAG 2 cut(s) 220, 279
DpnI GATC 3 cut(s) 75, 99, 139
DpnII GATC 3 cut(s) 73, 97, 137
Ecl136II GAGCTC 1 cut(s) 219
Eco24I GRGCYC 1 cut(s) 221
Eco53kI GAGCTC 1 cut(s) 219
Eco57I CTGAAG 2 cut(s) 33, 470
EcoICRI GAGCTC 1 cut(s) 219
EcoT38I GRGCYC 1 cut(s) 221
Esp3I CGTCTC 1 cut(s) 40
FaeI CATG 2 cut(s) 236, 263
FaiI YATR 4 cut(s) 234, 261, 312, 321
FatI CATG 2 cut(s) 232, 259
FauI CCCGC 1 cut(s) 356
FblI GTMKAC 1 cut(s) 160
Fnu4HI GCNGC 2 cut(s) 6, 480
FriOI GRGCYC 1 cut(s) 221
Fsp4HI GCNGC 2 cut(s) 6, 480
FspBI CTAG 1 cut(s) 532
GluI GCNGC 2 cut(s) 6, 480
GsuI CTGGAG 1 cut(s) 193
HaeIII GGCC 1 cut(s) 367
HgaI GACGC 1 cut(s) 171
Hin1I GRCGYC 2 cut(s) 33, 163
Hin1II CATG 2 cut(s) 236, 263
HincII GTYRAC 1 cut(s) 457
HindII GTYRAC 1 cut(s) 457
HinfI GANTC 7 cut(s) 44, 53, 110, 179, 188, 203, 301
HphI GGTGA 1 cut(s) 422
Hpy166II GTNNAC 3 cut(s) 161, 413, 457
Hpy188I TCNGA 7 cut(s) 31, 43, 52, 178, 187, 202, 223
Hpy188III TCNNGA 2 cut(s) 101, 243
Hpy8I GTNNAC 3 cut(s) 161, 413, 457
Hpy99I CGWCG 2 cut(s) 35, 66
HpyAV CCTTC 5 cut(s) 240, 268, 341, 347, 445
HpyCH4IV ACGT 2 cut(s) 33, 415
HpyCH4V TGCA 1 cut(s) 482
HpyF10VI GCNNNNNNNGC 3 cut(s) 162, 256, 265
HpyF3I CTNAG 2 cut(s) 220, 279
HpySE526I ACGT 2 cut(s) 33, 415
Hsp92I GRCGYC 2 cut(s) 33, 163
Hsp92II CATG 2 cut(s) 236, 263
Kzo9I GATC 3 cut(s) 73, 97, 137
LpnPI CCDG 3 cut(s) 223, 381, 417
Lsp1109I GCAGC 1 cut(s) 466
MaeI CTAG 1 cut(s) 532
MaeII ACGT 2 cut(s) 33, 415
MaeIII GTNAC 1 cut(s) 240
MalI GATC 3 cut(s) 75, 99, 139
MboI GATC 3 cut(s) 73, 97, 137
MboII GAAGA 4 cut(s) 9, 15, 39, 183
MhlI GDGCHC 1 cut(s) 221
MluCI AATT 1 cut(s) 441
MlyI GAGTC 3 cut(s) 47, 104, 310
MmeI TCCRAC 1 cut(s) 54
MnlI CCTC 7 cut(s) 37, 49, 154, 184, 193, 217, 463
MseI TTAA 2 cut(s) 507, 515
MspCI CTTAAG 1 cut(s) 506
MvnI CGCG 1 cut(s) 396
MwoI GCNNNNNNNGC 3 cut(s) 162, 256, 265
NdeII GATC 3 cut(s) 73, 97, 137
NlaIII CATG 2 cut(s) 236, 263
NmuCI GTSAC 1 cut(s) 240
PcsI WCGNNNNNNNCGW 1 cut(s) 525
PfeI GAWTC 4 cut(s) 44, 179, 188, 203
PkrI GCNGC 2 cut(s) 7, 481
Ple19I CGATCG 1 cut(s) 100
PleI GAGTC 3 cut(s) 47, 104, 309
PpsI GAGTC 3 cut(s) 47, 104, 309
Psp124BI GAGCTC 1 cut(s) 221
PspPI GGNCC 1 cut(s) 365
PstI CTGCAG 1 cut(s) 484
PvuI CGATCG 1 cut(s) 100
RsaI GTAC 1 cut(s) 123
RsaNI GTAC 1 cut(s) 122
SacI GAGCTC 1 cut(s) 221
SaqAI TTAA 2 cut(s) 507, 515
SatI GCNGC 2 cut(s) 6, 480
Sau3AI GATC 3 cut(s) 73, 97, 137
Sau96I GGNCC 1 cut(s) 365
SchI GAGTC 3 cut(s) 47, 104, 310
SduI GDGCHC 1 cut(s) 221
SetI ASST 6 cut(s) 36, 146, 221, 418, 456, 481
SfcI CTRYAG 1 cut(s) 480
SmlI CTYRAG 1 cut(s) 506
SmoI CTYRAG 1 cut(s) 506
Sse9I AATT 1 cut(s) 441
SsiI CCGC 3 cut(s) 5, 363, 394
SspMI CTAG 1 cut(s) 532
SstI GAGCTC 1 cut(s) 221
TaiI ACGT 2 cut(s) 36, 418
TaqI TCGA 3 cut(s) 100, 146, 528
TasI AATT 1 cut(s) 441
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 4 cut(s) 44, 179, 188, 203
Tru1I TTAA 2 cut(s) 507, 515
Tru9I TTAA 2 cut(s) 507, 515
TscAI CASTG 1 cut(s) 217
TseFI GTSAC 1 cut(s) 240
TseI GCWGC 1 cut(s) 479
Tsp45I GTSAC 1 cut(s) 240
TspDTI ATGAA 2 cut(s) 276, 513
TspGWI ACGGA 1 cut(s) 139
TspRI CASTG 1 cut(s) 217
Vha464I CTTAAG 1 cut(s) 506
XapI RAATTY 1 cut(s) 441
XmiI GTMKAC 1 cut(s) 160
XspI CTAG 1 cut(s) 532
ZraI GACGTC 1 cut(s) 34
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.