AT5G28830

Calcium-binding EF hand family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0014521
Physical Location & Seq
Forward (+)
10856661 .. 10858687
2027 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G28830.1

Sequence Viewer

Length: 975 bp
ATGTCGAACACGGCGGGATTAACGATTTTCGACGGTGATCTACTTAGATCCATCGATCTAAACCTACCGGAACACCAACACAGAGTCACCGGAGCTCAACTTCTCGAGATCTCTGAATCAAAAGTTTCTCAATCTCTCTCTGGTCTCTCACTACCACCACATCTCAAAGAAACAGCGATTTCTCAAGTCTCCGATGGAGATCACGTCACTTTCCGTCGTACGATGTTCAATAAACAACAAGCTTCTGAGAAACTCGGAGTTTTCTTCTCCACCGTCGCCGATGCTCTCAAAGATACACCAATTGTGGTGTCAATATTGGATGGGACTATGTTGAAGATGTTTTTGGAGGATGAAGATGATTTTGCAATGTTGGCAGAGAATTTGTTCACTGATTTAGATGAAGAAGATAAAGGAAAGCTTTGTAAGAGTGAGATACGAAAAGCTCTTGTACATATGGGTGTTGAAATGGGTGTTCCTCCACTCTCAGAATTTCCCATATTAGATGATATCATTAAGAAGCATGATGCTGATAGTGATGAAGAGCTTGGACAAGCACAATTTGCAGAACTACTGCAACAAGTTCTACAAGAAATAGCAGATGTCCTTCATGAGAAACCAATAACTATTGTCCTAAACGTTGAGATTTTCACTGGATCAAGGATTCGTAAGATTCTTGCTGATGAGAAGACACTCAAGTGTCTTGTAGAGAAAACGATTTTGGAAGAGTCGAACGGAAAAGAGAGCCAAGGATGGTTAAGGACGTTGATAATAAAGAATGGGAAAGAGTTAGGATTGCCTCCGTTATCTTCAGAAAACGAACAAGTGGCTCTTCTCTATGAGACCATAATTTCTCAGTTGAATAACAAAGAGAATGCTTCTACAAAAGAGGAGTTTATGGATGCTCTAAAGGATATACTTAAGAAATTTGAGGAGTTGCTCGAAACAACACCGGTGTTTTCTGCTATCAATCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5 EF-hand_7

Protein Analysis

324

Amino Acids

36.16

Weight (kDa)

4.67

Isoelectric Point (pI)

42.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014521)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G28830
fragaria_vesca FvH4_6g02990
malus_domestica MD04G1224000.v1.1 MD12G1240800.v1.1
prunus_persica Prupe.6G342700_v2.0.a1
pyrus_communis pycom04g19740 pycom12g22050
rosa_chinensis RchiOBHm_Chr3g0451171
rosa_laevigata RLG00000025680
rosa_multiflora Rmu_co8512575.1_g000001
rosa_roxburghii Rroxscaffold_6G00428640
rosa_rugosa Rorug02G0632500
rosa_samantha Rh3AG035000 Rh3BG035400 Rh3CG034000 Rh3DG035000
rosa_wichuraiana Rw3G002570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 14
AclI AACGTT 1 cut(s) 636
AclWI GGATC 2 cut(s) 42, 661
AcsI RAATTY 3 cut(s) 379, 488, 923
AcuI CTGAAG 1 cut(s) 792
AfaI GTAC 2 cut(s) 220, 450
AflII CTTAAG 1 cut(s) 917
AgeI ACCGGT 1 cut(s) 949
AgsI TTSAA 4 cut(s) 229, 334, 464, 859
AjiI CACGTC 1 cut(s) 205
AjuI GAANNNNNNNTTGG 4 cut(s) 326, 358, 701, 733
AleI CACNNNNGTG 1 cut(s) 694
AluBI AGCT 5 cut(s) 95, 242, 418, 443, 544
AluI AGCT 5 cut(s) 95, 242, 418, 443, 544
Alw21I GWGCWC 1 cut(s) 97
Alw26I GTCTC 3 cut(s) 149, 193, 833
AlwI GGATC 2 cut(s) 42, 661
Ama87I CYCGRG 1 cut(s) 104
ApoI RAATTY 3 cut(s) 379, 488, 923
AsiGI ACCGGT 1 cut(s) 949
Asp700I GAANNNNTTC 1 cut(s) 383
AsuHPI GGTGA 2 cut(s) 47, 79
AvaI CYCGRG 1 cut(s) 104
BanII GRGCYC 1 cut(s) 97
BbsI GAAGAC 1 cut(s) 692
Bbv12I GWGCWC 1 cut(s) 97
BccI CCATC 4 cut(s) 59, 188, 314, 744
BceAI ACGGC 1 cut(s) 27
BcoDI GTCTC 3 cut(s) 149, 193, 833
BfaI CTAG 1 cut(s) 973
BfrI CTTAAG 1 cut(s) 917
BglII AGATCT 1 cut(s) 108
BmeT110I CYCGRG 1 cut(s) 104
BmgBI CACGTC 1 cut(s) 205
BmsI GCATC 3 cut(s) 271, 514, 889
BpiI GAAGAC 1 cut(s) 692
BpuEI CTTGAG 2 cut(s) 168, 677
Bsa29I ATCGAT 1 cut(s) 54
BsaBI GATNNNNATC 1 cut(s) 198
BsaI GGTCTC 2 cut(s) 149, 833
BsaJI CCNNGG 1 cut(s) 745
BsaWI WCCGGW 3 cut(s) 67, 89, 949
BsaXI ACNNNNNCTCC 2 cut(s) 189, 219
Bse118I RCCGGY 1 cut(s) 949
Bse1I ACTGG 1 cut(s) 655
Bse3DI GCAATG 1 cut(s) 372
Bse8I GATNNNNATC 1 cut(s) 198
BseCI ATCGAT 1 cut(s) 54
BseDI CCNNGG 1 cut(s) 745
BseGI GGATG 4 cut(s) 325, 355, 755, 904
BseJI GATNNNNATC 1 cut(s) 198
BseMI GCAATG 1 cut(s) 372
BseMII CTCAG 3 cut(s) 237, 498, 866
BseNI ACTGG 1 cut(s) 655
BseRI GAGGAG 2 cut(s) 902, 944
BshTI ACCGGT 1 cut(s) 949
BshVI ATCGAT 1 cut(s) 54
BsiHKAI GWGCWC 1 cut(s) 97
BsiHKCI CYCGRG 1 cut(s) 104
BsiSI CCGG 3 cut(s) 68, 90, 950
BsiWI CGTACG 1 cut(s) 218
BslFI GGGAC 1 cut(s) 337
BsmAI GTCTC 3 cut(s) 149, 193, 833
BsmFI GGGAC 1 cut(s) 337
BsmI GAATGC 1 cut(s) 877
Bso31I GGTCTC 2 cut(s) 149, 833
BsoBI CYCGRG 1 cut(s) 104
Bsp1286I GDGCHC 1 cut(s) 97
Bsp1407I TGTACA 1 cut(s) 448
Bsp143I GATC 6 cut(s) 37, 47, 55, 108, 199, 653
BspACI CCGC 1 cut(s) 14
BspCNI CTCAG 3 cut(s) 238, 497, 865
BspDI ATCGAT 1 cut(s) 54
BspHI TCATGA 1 cut(s) 607
BspPI GGATC 2 cut(s) 42, 661
BspQI GCTCTTC 2 cut(s) 534, 834
BspTI CTTAAG 1 cut(s) 917
BspTNI GGTCTC 2 cut(s) 149, 833
BsrDI GCAATG 1 cut(s) 372
BsrFI RCCGGY 1 cut(s) 949
BsrGI TGTACA 1 cut(s) 448
BsrI ACTGG 1 cut(s) 655
BssAI RCCGGY 1 cut(s) 949
BssECI CCNNGG 1 cut(s) 745
BssMI GATC 6 cut(s) 37, 47, 55, 108, 199, 653
BssT1I CCWWGG 1 cut(s) 745
Bst4CI ACNGT 2 cut(s) 35, 274
Bst6I CTCTTC 3 cut(s) 534, 717, 834
BstAFI CTTAAG 1 cut(s) 917
BstAPI GCANNNNNTGC 1 cut(s) 560
BstAUI TGTACA 1 cut(s) 448
BstDEI CTNAG 4 cut(s) 44, 246, 484, 852
BstF5I GGATG 4 cut(s) 325, 355, 755, 904
BstKTI GATC 6 cut(s) 40, 50, 58, 111, 202, 656
BstMAI GTCTC 3 cut(s) 149, 193, 833
BstMBI GATC 6 cut(s) 37, 47, 55, 108, 199, 653
BstMWI GCNNNNNNNGC 2 cut(s) 371, 560
BstV2I GAAGAC 1 cut(s) 692
BstX2I RGATCY 2 cut(s) 47, 108
BstYI RGATCY 2 cut(s) 47, 108
Bsu15I ATCGAT 1 cut(s) 54
BsuTUI ATCGAT 1 cut(s) 54
BtrI CACGTC 1 cut(s) 205
BtsCI GGATG 4 cut(s) 325, 355, 755, 904
BtsIMutI CAGTG 2 cut(s) 387, 648
CciI TCATGA 1 cut(s) 607
Cfr10I RCCGGY 1 cut(s) 949
ClaI ATCGAT 1 cut(s) 54
Csp6I GTAC 2 cut(s) 219, 449
CspAI ACCGGT 1 cut(s) 949
CviAII CATG 2 cut(s) 521, 608
CviJI RGCY 7 cut(s) 95, 242, 418, 443, 544, 744, 827
CviKI_1 RGCY 7 cut(s) 95, 242, 418, 443, 544, 744, 827
CviQI GTAC 2 cut(s) 219, 449
DdeI CTNAG 4 cut(s) 44, 246, 484, 852
DpnI GATC 6 cut(s) 39, 49, 57, 110, 201, 655
DpnII GATC 6 cut(s) 37, 47, 55, 108, 199, 653
Eam1104I CTCTTC 3 cut(s) 534, 717, 834
EarI CTCTTC 3 cut(s) 534, 717, 834
Ecl136II GAGCTC 1 cut(s) 95
Eco130I CCWWGG 1 cut(s) 745
Eco24I GRGCYC 1 cut(s) 97
Eco31I GGTCTC 2 cut(s) 149, 833
Eco32I GATATC 1 cut(s) 508
Eco53kI GAGCTC 1 cut(s) 95
Eco57I CTGAAG 1 cut(s) 792
Eco88I CYCGRG 1 cut(s) 104
EcoICRI GAGCTC 1 cut(s) 95
EcoRV GATATC 1 cut(s) 508
EcoT14I CCWWGG 1 cut(s) 745
EcoT38I GRGCYC 1 cut(s) 97
ErhI CCWWGG 1 cut(s) 745
FaeI CATG 2 cut(s) 524, 611
FalI AAGNNNNNCTT 4 cut(s) 402, 434, 813, 845
FaqI GGGAC 1 cut(s) 337
FatI CATG 2 cut(s) 520, 607
FauI CCCGC 1 cut(s) 7
FauNDI CATATG 1 cut(s) 453
FokI GGATG 4 cut(s) 332, 362, 762, 911
FriOI GRGCYC 1 cut(s) 97
FspBI CTAG 1 cut(s) 973
HapII CCGG 3 cut(s) 68, 90, 950
Hin1II CATG 2 cut(s) 524, 611
HindIII AAGCTT 2 cut(s) 240, 416
HinfI GANTC 5 cut(s) 84, 116, 661, 670, 725
HpaII CCGG 3 cut(s) 68, 90, 950
HphI GGTGA 2 cut(s) 47, 79
Hpy166II GTNNAC 1 cut(s) 387
Hpy188I TCNGA 6 cut(s) 115, 193, 247, 257, 487, 811
Hpy188III TCNNGA 3 cut(s) 104, 106, 608
Hpy8I GTNNAC 1 cut(s) 387
Hpy99I CGWCG 3 cut(s) 35, 219, 278
HpyAV CCTTC 1 cut(s) 614
HpyCH4III ACNGT 2 cut(s) 35, 274
HpyCH4IV ACGT 3 cut(s) 204, 636, 761
HpyCH4V TGCA 3 cut(s) 365, 563, 574
HpyF10VI GCNNNNNNNGC 2 cut(s) 371, 560
HpyF3I CTNAG 4 cut(s) 44, 246, 484, 852
HpySE526I ACGT 3 cut(s) 204, 636, 761
Hsp92II CATG 2 cut(s) 524, 611
Kzo9I GATC 6 cut(s) 37, 47, 55, 108, 199, 653
LguI GCTCTTC 2 cut(s) 534, 834
LmnI GCTCC 1 cut(s) 92
LpnPI CCDG 5 cut(s) 81, 103, 126, 636, 963
LweI GCATC 3 cut(s) 271, 514, 889
MaeI CTAG 1 cut(s) 973
MaeII ACGT 3 cut(s) 204, 636, 761
MaeIII GTNAC 2 cut(s) 85, 205
MalI GATC 6 cut(s) 39, 49, 57, 110, 201, 655
MboI GATC 6 cut(s) 37, 47, 55, 108, 199, 653
MfeI CAATTG 1 cut(s) 300
MflI RGATCY 2 cut(s) 47, 108
MhlI GDGCHC 1 cut(s) 97
MluCI AATT 6 cut(s) 300, 379, 488, 557, 846, 923
MlyI GAGTC 2 cut(s) 93, 734
MnlI CCTC 5 cut(s) 340, 486, 807, 880, 922
MroXI GAANNNNTTC 1 cut(s) 383
MseI TTAA 4 cut(s) 20, 513, 755, 918
MslI CAYNNNNRTG 2 cut(s) 456, 694
MspCI CTTAAG 1 cut(s) 917
MspI CCGG 3 cut(s) 68, 90, 950
MunI CAATTG 1 cut(s) 300
Mva1269I GAATGC 1 cut(s) 877
MwoI GCNNNNNNNGC 2 cut(s) 371, 560
NdeI CATATG 1 cut(s) 453
NdeII GATC 6 cut(s) 37, 47, 55, 108, 199, 653
NlaIII CATG 2 cut(s) 524, 611
NmuCI GTSAC 2 cut(s) 85, 205
OliI CACNNNNGTG 1 cut(s) 694
PaeR7I CTCGAG 1 cut(s) 104
PagI TCATGA 1 cut(s) 607
PciSI GCTCTTC 2 cut(s) 534, 834
PctI GAATGC 1 cut(s) 877
PdmI GAANNNNTTC 1 cut(s) 383
PfeI GAWTC 3 cut(s) 116, 661, 670
Pfl23II CGTACG 1 cut(s) 218
PinAI ACCGGT 1 cut(s) 949
PleI GAGTC 2 cut(s) 92, 733
PpsI GAGTC 2 cut(s) 92, 733
Psp124BI GAGCTC 1 cut(s) 97
Psp1406I AACGTT 1 cut(s) 636
PspLI CGTACG 1 cut(s) 218
PsuI RGATCY 2 cut(s) 47, 108
RsaI GTAC 2 cut(s) 220, 450
RsaNI GTAC 2 cut(s) 219, 449
RseI CAYNNNNRTG 2 cut(s) 456, 694
SacI GAGCTC 1 cut(s) 97
SapI GCTCTTC 2 cut(s) 534, 834
SaqAI TTAA 4 cut(s) 20, 513, 755, 918
Sau3AI GATC 6 cut(s) 37, 47, 55, 108, 199, 653
SchI GAGTC 2 cut(s) 93, 734
SduI GDGCHC 1 cut(s) 97
SetI ASST 9 cut(s) 66, 97, 207, 244, 420, 445, 546, 639, 764
SfaNI GCATC 3 cut(s) 271, 514, 889
Sfr274I CTCGAG 1 cut(s) 104
SgrAI CRCCGGYG 1 cut(s) 949
SlaI CTCGAG 1 cut(s) 104
SmiMI CAYNNNNRTG 2 cut(s) 456, 694
SmlI CTYRAG 4 cut(s) 104, 183, 692, 917
SmoI CTYRAG 4 cut(s) 104, 183, 692, 917
Sse9I AATT 6 cut(s) 300, 379, 488, 557, 846, 923
SsiI CCGC 1 cut(s) 14
SspI AATATT 1 cut(s) 315
SspMI CTAG 1 cut(s) 973
SstI GAGCTC 1 cut(s) 97
StyI CCWWGG 1 cut(s) 745
TaaI ACNGT 2 cut(s) 35, 274
TaiI ACGT 3 cut(s) 207, 639, 764
TaqI TCGA 6 cut(s) 5, 30, 54, 105, 728, 939
TasI AATT 6 cut(s) 300, 379, 488, 557, 846, 923
TatI WGTACW 1 cut(s) 448
TfiI GAWTC 3 cut(s) 116, 661, 670
Tru1I TTAA 4 cut(s) 20, 513, 755, 918
Tru9I TTAA 4 cut(s) 20, 513, 755, 918
TscAI CASTG 2 cut(s) 394, 655
TseFI GTSAC 2 cut(s) 85, 205
Tsp45I GTSAC 2 cut(s) 85, 205
TspDTI ATGAA 4 cut(s) 366, 414, 552, 596
TspGWI ACGGA 3 cut(s) 203, 747, 789
TspRI CASTG 2 cut(s) 394, 655
Vha464I CTTAAG 1 cut(s) 917
XapI RAATTY 3 cut(s) 379, 488, 923
XhoI CTCGAG 1 cut(s) 104
XmnI GAANNNNTTC 1 cut(s) 383
XspI CTAG 1 cut(s) 973
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.