MD04G1224000.v1.1

Calcium-binding EF hand family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0014521
Physical Location & Seq
Forward (+)
30405162 .. 30408707
3546 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1224000.v1.1.491

Sequence Viewer

Length: 975 bp
ATGTCGGACGGAGGCTTAACGGTCCTCGACGGCAGCCACCTGAGAGGCGTCGACCTCATCCTGCCGGATGCTGACGCCAGCCTAACGGGAGCTCAGGTCCTCGACCTTGCCGTCTCCAAAGTCTCTTCCTCCCTCTTCGGCCTCTCGCTGCCTCAGAGCCTCAAGTCCTCTGCTTTGAGACGCAACAGCCTCCAAGACGACGACGCTTTCCGAAGCACGGAGCTCGATCGCGCGCAGGCTCTGAAGGTGATCGGCGAGTACATCACGGCGATCGCCGATGAATTGAAAGATGATCCGCTTGTCATATCTGTCTTGGACGGATGCAATCTTCGACTGTTTTTAGAGGACGAGGATGATTTTGCAATGTTAGCGGAGAATCTGTTCACTGATCTGGATGTAGAAGATAAAGGAAAGATCAACAAGAAGGAGATCCGGAATGCGCTTGCTCACATGGGAGTTGAGATGGGCGTTCCTCCCGTTTCAGAATTTCCTCCTCTAAGTGATATCTTGACAAAACACGAAGCTGATGGGGATGAAGAATTGGGCCAAGCACAATTTGCTCAGCTACTCCAGCCTGTCCTGCAGGAACTAGCCGAGGCCCTGGCCAAAAAGCATGTTGTTATCATCCAGAGTGTCAAGATTGTAAATGGTTCCAAGCTAAGAAAGTTTTTGGCTGATGAGAAGCAATTGAAAAATGTGGTTGAGAAGATATTGCAAGAAGAGCACCATGGAAAGGACGGATTGGGGAATGCAGATAAAATTAGGAGTTTTCTTGAGAAAAATGGGCAAGAGTTGGGTCTGCCACCATACGAACCCGAGGCAGTTACTCTTTTGTACGATGCAGTGTTTGCTGATCTAAGCGCAGATAAGAGTGCTCCGGAAGCAGGTCAAGATGAATGTGGAAATCTTGTGAAGGAGATACTCGAGGAATTCGCAGATCAGCTAGAAGCGAGTCCTGTTTTTTGTGATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5 EF-hand_7

Protein Analysis

325

Amino Acids

35.23

Weight (kDa)

4.44

Isoelectric Point (pI)

43.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014521)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G28830
fragaria_vesca FvH4_6g02990
malus_domestica MD04G1224000.v1.1 MD12G1240800.v1.1
prunus_persica Prupe.6G342700_v2.0.a1
pyrus_communis pycom04g19740 pycom12g22050
rosa_chinensis RchiOBHm_Chr3g0451171
rosa_laevigata RLG00000025680
rosa_multiflora Rmu_co8512575.1_g000001
rosa_roxburghii Rroxscaffold_6G00428640
rosa_rugosa Rorug02G0632500
rosa_samantha Rh3AG035000 Rh3BG035400 Rh3CG034000 Rh3DG035000
rosa_wichuraiana Rw3G002570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 110
Acc36I ACCTGC 1 cut(s) 875
AccI GTMKAC 1 cut(s) 51
AccII CGCG 2 cut(s) 231, 233
AccIII TCCGGA 2 cut(s) 432, 877
AciI CCGC 2 cut(s) 296, 371
AclWI GGATC 2 cut(s) 287, 424
AcoI YGGCCR 1 cut(s) 603
AcsI RAATTY 2 cut(s) 485, 929
AcuI CTGAAG 1 cut(s) 263
AcyI GRCGYC 2 cut(s) 48, 75
AfaI GTAC 2 cut(s) 260, 836
AfiI CCNNNNNNNGG 3 cut(s) 217, 733, 884
AgsI TTSAA 2 cut(s) 286, 691
AjnI CCWGG 1 cut(s) 600
AloI GAACNNNNNNTCC 2 cut(s) 365, 397
AluBI AGCT 6 cut(s) 92, 223, 524, 565, 658, 943
AluI AGCT 6 cut(s) 92, 223, 524, 565, 658, 943
Alw21I GWGCWC 4 cut(s) 94, 225, 726, 877
Alw26I GTCTC 3 cut(s) 118, 127, 172
AlwI GGATC 2 cut(s) 287, 424
AlwNI CAGNNNCTG 1 cut(s) 241
Ama87I CYCGRG 2 cut(s) 815, 923
Aor13HI TCCGGA 2 cut(s) 432, 877
AoxI GGCC 4 cut(s) 139, 544, 597, 603
ApeKI GCWGC 2 cut(s) 33, 148
ApoI RAATTY 2 cut(s) 485, 929
AsiSI GCGATCGC 1 cut(s) 273
Asp700I GAANNNNTTC 1 cut(s) 380
AspLEI GCGC 4 cut(s) 233, 235, 442, 863
AspS9I GGNCC 4 cut(s) 22, 97, 544, 598
AsuHPI GGTGA 1 cut(s) 259
AvaI CYCGRG 2 cut(s) 815, 923
AvaII GGWCC 2 cut(s) 22, 97
BalI TGGCCA 1 cut(s) 605
BanII GRGCYC 2 cut(s) 94, 225
Bbv12I GWGCWC 4 cut(s) 94, 225, 726, 877
BbvI GCAGC 2 cut(s) 45, 135
BccI CCATC 2 cut(s) 457, 521
BceAI ACGGC 3 cut(s) 46, 95, 282
BcgI CGANNNNNNTGC 2 cut(s) 205, 239
BciT130I CCWGG 1 cut(s) 602
BcoDI GTCTC 3 cut(s) 118, 127, 172
BfaI CTAG 2 cut(s) 590, 944
BfmI CTRYAG 1 cut(s) 581
BfuAI ACCTGC 1 cut(s) 875
BisI GCNGC 2 cut(s) 34, 149
BlpI GCTNAGC 1 cut(s) 561
BlsI GCNGC 2 cut(s) 35, 150
Bme1390I CCNGG 1 cut(s) 602
Bme18I GGWCC 2 cut(s) 22, 97
BmeT110I CYCGRG 2 cut(s) 815, 923
BmgT120I GGNCC 4 cut(s) 22, 97, 544, 598
BmiI GGNNCC 1 cut(s) 652
BmrFI CCNGG 1 cut(s) 602
BmsI GCATC 4 cut(s) 58, 311, 829, 958
BpmI CTGGAG 1 cut(s) 554
Bpu10I CCTNAGC 1 cut(s) 93
Bpu1102I GCTNAGC 1 cut(s) 561
BpuEI CTTGAG 2 cut(s) 146, 794
BsaHI GRCGYC 2 cut(s) 48, 75
BsaJI CCNNGG 4 cut(s) 594, 600, 727, 816
BsaWI WCCGGW 2 cut(s) 432, 877
BsaXI ACNNNNNCTCC 4 cut(s) 81, 111, 365, 395
Bsc4I CCNNNNNNNGG 3 cut(s) 217, 733, 884
Bse3DI GCAATG 1 cut(s) 369
BseAI TCCGGA 2 cut(s) 432, 877
BseBI CCWGG 1 cut(s) 602
BseDI CCNNGG 4 cut(s) 594, 600, 727, 816
BseGI GGATG 7 cut(s) 57, 73, 326, 358, 400, 538, 624
BseLI CCNNNNNNNGG 3 cut(s) 217, 733, 884
BseMI GCAATG 1 cut(s) 369
BseMII CTCAG 4 cut(s) 32, 107, 167, 575
BsePI GCGCGC 1 cut(s) 231
BseRI GAGGAG 1 cut(s) 483
BseXI GCAGC 2 cut(s) 45, 135
Bsh1236I CGCG 2 cut(s) 231, 233
Bsh1285I CGRYCG 2 cut(s) 229, 273
BshFI GGCC 4 cut(s) 141, 546, 599, 605
BsiEI CGRYCG 2 cut(s) 229, 273
BsiHKAI GWGCWC 4 cut(s) 94, 225, 726, 877
BsiHKCI CYCGRG 2 cut(s) 815, 923
BsiSI CCGG 3 cut(s) 65, 433, 878
BslI CCNNNNNNNGG 3 cut(s) 217, 733, 884
BsmAI GTCTC 3 cut(s) 118, 127, 172
BsmBI CGTCTC 2 cut(s) 118, 172
BsmI GAATGC 2 cut(s) 442, 754
BsnI GGCC 4 cut(s) 141, 546, 599, 605
BsoBI CYCGRG 2 cut(s) 815, 923
Bsp1286I GDGCHC 4 cut(s) 94, 225, 726, 877
Bsp13I TCCGGA 2 cut(s) 432, 877
Bsp143I GATC 9 cut(s) 226, 249, 270, 292, 388, 414, 429, 853, 937
Bsp1720I GCTNAGC 1 cut(s) 561
Bsp19I CCATGG 1 cut(s) 727
BspACI CCGC 2 cut(s) 296, 371
BspANI GGCC 4 cut(s) 141, 546, 599, 605
BspCNI CTCAG 4 cut(s) 33, 106, 166, 574
BspEI TCCGGA 2 cut(s) 432, 877
BspFNI CGCG 2 cut(s) 231, 233
BspLI GGNNCC 1 cut(s) 652
BspMAI CTGCAG 1 cut(s) 585
BspMI ACCTGC 1 cut(s) 875
BspPI GGATC 2 cut(s) 287, 424
BspQI GCTCTTC 1 cut(s) 714
BsrDI GCAATG 1 cut(s) 369
BssECI CCNNGG 4 cut(s) 594, 600, 727, 816
BssHII GCGCGC 1 cut(s) 231
BssMI GATC 9 cut(s) 226, 249, 270, 292, 388, 414, 429, 853, 937
BssNI GRCGYC 2 cut(s) 48, 75
BssT1I CCWWGG 1 cut(s) 727
Bst2UI CCWGG 1 cut(s) 602
Bst4CI ACNGT 2 cut(s) 22, 336
Bst6I CTCTTC 3 cut(s) 130, 140, 714
BstACI GRCGYC 2 cut(s) 48, 75
BstAPI GCANNNNNTGC 2 cut(s) 557, 848
BstC8I GCNNGC 4 cut(s) 79, 233, 237, 444
BstDEI CTNAG 7 cut(s) 41, 93, 153, 497, 561, 659, 857
BstDSI CCRYGG 1 cut(s) 727
BstF5I GGATG 7 cut(s) 57, 73, 326, 358, 400, 538, 624
BstFNI CGCG 2 cut(s) 231, 233
BstHHI GCGC 4 cut(s) 233, 235, 442, 863
BstKTI GATC 9 cut(s) 229, 252, 273, 295, 391, 417, 432, 856, 940
BstMAI GTCTC 3 cut(s) 118, 127, 172
BstMBI GATC 9 cut(s) 226, 249, 270, 292, 388, 414, 429, 853, 937
BstMCI CGRYCG 2 cut(s) 229, 273
BstMWI GCNNNNNNNGC 7 cut(s) 368, 557, 571, 580, 721, 848, 881
BstNI CCWGG 1 cut(s) 602
BstNSI RCATGY 1 cut(s) 617
BstSCI CCNGG 1 cut(s) 600
BstSFI CTRYAG 1 cut(s) 581
BstUI CGCG 2 cut(s) 231, 233
BstV1I GCAGC 2 cut(s) 45, 135
BstX2I RGATCY 1 cut(s) 429
BstYI RGATCY 1 cut(s) 429
BsuRI GGCC 4 cut(s) 141, 546, 599, 605
BtgI CCRYGG 1 cut(s) 727
BtsCI GGATG 7 cut(s) 57, 73, 326, 358, 400, 538, 624
BtsI GCAGTG 1 cut(s) 849
BtsIMutI CAGTG 2 cut(s) 384, 849
BveI ACCTGC 1 cut(s) 875
Cac8I GCNNGC 4 cut(s) 79, 233, 237, 444
CaiI CAGNNNCTG 1 cut(s) 241
CfoI GCGC 4 cut(s) 233, 235, 442, 863
Cfr13I GGNCC 4 cut(s) 22, 97, 544, 598
CseI GACGC 4 cut(s) 37, 83, 189, 212
Csp6I GTAC 2 cut(s) 259, 835
CviAII CATG 3 cut(s) 451, 614, 728
CviQI GTAC 2 cut(s) 259, 835
DdeI CTNAG 7 cut(s) 41, 93, 153, 497, 561, 659, 857
DpnI GATC 9 cut(s) 228, 251, 272, 294, 390, 416, 431, 855, 939
DpnII GATC 9 cut(s) 226, 249, 270, 292, 388, 414, 429, 853, 937
DrdI GACNNNNNNGTC 1 cut(s) 110
DseDI GACNNNNNNGTC 1 cut(s) 110
EaeI YGGCCR 1 cut(s) 603
Eam1104I CTCTTC 3 cut(s) 130, 140, 714
EarI CTCTTC 3 cut(s) 130, 140, 714
Ecl136II GAGCTC 2 cut(s) 92, 223
Eco130I CCWWGG 1 cut(s) 727
Eco24I GRGCYC 2 cut(s) 94, 225
Eco32I GATATC 1 cut(s) 505
Eco47I GGWCC 2 cut(s) 22, 97
Eco53kI GAGCTC 2 cut(s) 92, 223
Eco57I CTGAAG 1 cut(s) 263
Eco88I CYCGRG 2 cut(s) 815, 923
EcoICRI GAGCTC 2 cut(s) 92, 223
EcoO109I RGGNCCY 2 cut(s) 97, 598
EcoRI GAATTC 1 cut(s) 929
EcoRII CCWGG 1 cut(s) 600
EcoRV GATATC 1 cut(s) 505
EcoT14I CCWWGG 1 cut(s) 727
EcoT38I GRGCYC 2 cut(s) 94, 225
ErhI CCWWGG 1 cut(s) 727
Esp3I CGTCTC 2 cut(s) 118, 172
FaeI CATG 3 cut(s) 454, 617, 731
FaiI YATR 5 cut(s) 305, 452, 615, 729, 808
FatI CATG 3 cut(s) 450, 613, 727
FblI GTMKAC 1 cut(s) 51
Fnu4HI GCNGC 2 cut(s) 34, 149
FokI GGATG 7 cut(s) 44, 80, 333, 365, 407, 545, 611
FriOI GRGCYC 2 cut(s) 94, 225
Fsp4HI GCNGC 2 cut(s) 34, 149
FspBI CTAG 2 cut(s) 590, 944
GlaI GCGC 4 cut(s) 232, 234, 441, 862
GluI GCNGC 2 cut(s) 34, 149
GsuI CTGGAG 1 cut(s) 554
HaeIII GGCC 4 cut(s) 141, 546, 599, 605
HapII CCGG 3 cut(s) 65, 433, 878
HgaI GACGC 4 cut(s) 37, 83, 189, 212
HhaI GCGC 4 cut(s) 233, 235, 442, 863
Hin1I GRCGYC 2 cut(s) 48, 75
Hin1II CATG 3 cut(s) 454, 617, 731
Hin6I GCGC 4 cut(s) 231, 233, 440, 861
HinP1I GCGC 4 cut(s) 231, 233, 440, 861
HincII GTYRAC 1 cut(s) 52
HindII GTYRAC 1 cut(s) 52
HinfI GANTC 2 cut(s) 376, 952
HpaII CCGG 3 cut(s) 65, 433, 878
HphI GGTGA 1 cut(s) 259
Hpy166II GTNNAC 2 cut(s) 52, 384
Hpy188I TCNGA 5 cut(s) 7, 156, 212, 243, 484
Hpy188III TCNNGA 8 cut(s) 392, 433, 508, 628, 637, 773, 878, 890
Hpy8I GTNNAC 2 cut(s) 52, 384
Hpy99I CGWCG 4 cut(s) 32, 53, 203, 206
HpyAV CCTTC 3 cut(s) 238, 418, 907
HpyCH4III ACNGT 2 cut(s) 22, 336
HpyCH4V TGCA 6 cut(s) 324, 362, 583, 715, 752, 842
HpyF10VI GCNNNNNNNGC 7 cut(s) 368, 557, 571, 580, 721, 848, 881
HpyF3I CTNAG 7 cut(s) 41, 93, 153, 497, 561, 659, 857
Hsp92I GRCGYC 2 cut(s) 48, 75
Hsp92II CATG 3 cut(s) 454, 617, 731
HspAI GCGC 4 cut(s) 231, 233, 440, 861
Kpn2I TCCGGA 2 cut(s) 432, 877
Kzo9I GATC 9 cut(s) 226, 249, 270, 292, 388, 414, 429, 853, 937
LguI GCTCTTC 1 cut(s) 714
LmnI GCTCC 3 cut(s) 89, 220, 880
Lsp1109I GCAGC 2 cut(s) 45, 135
LweI GCATC 4 cut(s) 58, 311, 829, 958
MaeI CTAG 2 cut(s) 590, 944
MaeIII GTNAC 1 cut(s) 823
MalI GATC 9 cut(s) 228, 251, 272, 294, 390, 416, 431, 855, 939
MboI GATC 9 cut(s) 226, 249, 270, 292, 388, 414, 429, 853, 937
MboII GAAGA 7 cut(s) 117, 127, 320, 413, 548, 718, 731
MfeI CAATTG 1 cut(s) 686
MflI RGATCY 1 cut(s) 429
MhlI GDGCHC 4 cut(s) 94, 225, 726, 877
MlsI TGGCCA 1 cut(s) 605
MluCI AATT 7 cut(s) 281, 485, 539, 554, 686, 759, 929
MluNI TGGCCA 1 cut(s) 605
MlyI GAGTC 1 cut(s) 961
Mox20I TGGCCA 1 cut(s) 605
MroI TCCGGA 2 cut(s) 432, 877
MroXI GAANNNNTTC 1 cut(s) 380
MscI TGGCCA 1 cut(s) 605
MseI TTAA 1 cut(s) 17
Msp20I TGGCCA 1 cut(s) 605
MspI CCGG 3 cut(s) 65, 433, 878
MspR9I CCNGG 1 cut(s) 602
MunI CAATTG 1 cut(s) 686
Mva1269I GAATGC 2 cut(s) 442, 754
MvaI CCWGG 1 cut(s) 602
MvnI CGCG 2 cut(s) 231, 233
MwoI GCNNNNNNNGC 7 cut(s) 368, 557, 571, 580, 721, 848, 881
NcoI CCATGG 1 cut(s) 727
NdeII GATC 9 cut(s) 226, 249, 270, 292, 388, 414, 429, 853, 937
NlaIII CATG 3 cut(s) 454, 617, 731
NlaIV GGNNCC 1 cut(s) 652
NmeAIII GCCGAG 1 cut(s) 619
NspI RCATGY 1 cut(s) 617
PaeR7I CTCGAG 1 cut(s) 923
PauI GCGCGC 1 cut(s) 231
PciSI GCTCTTC 1 cut(s) 714
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PctI GAATGC 2 cut(s) 442, 754
PdmI GAANNNNTTC 1 cut(s) 380
PfeI GAWTC 1 cut(s) 376
PkrI GCNGC 2 cut(s) 35, 150
Ple19I CGATCG 2 cut(s) 229, 273
PleI GAGTC 1 cut(s) 960
PpsI GAGTC 1 cut(s) 960
PpuMI RGGWCCY 1 cut(s) 97
Psp124BI GAGCTC 2 cut(s) 94, 225
Psp5II RGGWCCY 1 cut(s) 97
Psp6I CCWGG 1 cut(s) 600
PspGI CCWGG 1 cut(s) 600
PspN4I GGNNCC 1 cut(s) 652
PspPI GGNCC 4 cut(s) 22, 97, 544, 598
PspPPI RGGWCCY 1 cut(s) 97
PspXI VCTCGAGB 1 cut(s) 923
PstI CTGCAG 1 cut(s) 585
PstNI CAGNNNCTG 1 cut(s) 241
PsuI RGATCY 1 cut(s) 429
PteI GCGCGC 1 cut(s) 231
PvuI CGATCG 2 cut(s) 229, 273
RgaI GCGATCGC 1 cut(s) 273
RsaI GTAC 2 cut(s) 260, 836
RsaNI GTAC 2 cut(s) 259, 835
SacI GAGCTC 2 cut(s) 94, 225
SalI GTCGAC 1 cut(s) 50
SapI GCTCTTC 1 cut(s) 714
SaqAI TTAA 1 cut(s) 17
SatI GCNGC 2 cut(s) 34, 149
Sau3AI GATC 9 cut(s) 226, 249, 270, 292, 388, 414, 429, 853, 937
Sau96I GGNCC 4 cut(s) 22, 97, 544, 598
SbfI CCTGCAGG 1 cut(s) 585
SchI GAGTC 1 cut(s) 961
ScrFI CCNGG 1 cut(s) 602
SdaI CCTGCAGG 1 cut(s) 585
SduI GDGCHC 4 cut(s) 94, 225, 726, 877
SfaAI GCGATCGC 1 cut(s) 273
SfaNI GCATC 4 cut(s) 58, 311, 829, 958
SfcI CTRYAG 1 cut(s) 581
Sfr274I CTCGAG 1 cut(s) 923
SgfI GCGATCGC 1 cut(s) 273
SinI GGWCC 2 cut(s) 22, 97
SlaI CTCGAG 1 cut(s) 923
SmlI CTYRAG 3 cut(s) 161, 773, 923
SmoI CTYRAG 3 cut(s) 161, 773, 923
Sse8387I CCTGCAGG 1 cut(s) 585
Sse9I AATT 7 cut(s) 281, 485, 539, 554, 686, 759, 929
SsiI CCGC 2 cut(s) 296, 371
SspMI CTAG 2 cut(s) 590, 944
SstI GAGCTC 2 cut(s) 94, 225
StyD4I CCNGG 1 cut(s) 600
StyI CCWWGG 1 cut(s) 727
TaaI ACNGT 2 cut(s) 22, 336
TaqI TCGA 6 cut(s) 27, 51, 102, 225, 331, 924
TasI AATT 7 cut(s) 281, 485, 539, 554, 686, 759, 929
TatI WGTACW 1 cut(s) 258
TfiI GAWTC 1 cut(s) 376
Tru1I TTAA 1 cut(s) 17
Tru9I TTAA 1 cut(s) 17
TscAI CASTG 2 cut(s) 391, 849
TseI GCWGC 2 cut(s) 33, 148
TspDTI ATGAA 3 cut(s) 294, 549, 909
TspGWI ACGGA 4 cut(s) 24, 233, 333, 753
TspRI CASTG 2 cut(s) 391, 849
VpaK11BI GGWCC 2 cut(s) 22, 97
XapI RAATTY 2 cut(s) 485, 929
XceI RCATGY 1 cut(s) 617
XhoI CTCGAG 1 cut(s) 923
XmiI GTMKAC 1 cut(s) 51
XmnI GAANNNNTTC 1 cut(s) 380
XspI CTAG 2 cut(s) 590, 944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.