Rh3BG035400

Calcium-binding EF hand family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0014521
Physical Location & Seq
Reverse (-)
2332725 .. 2337093
4369 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG035400.1

Sequence Viewer

Length: 777 bp
ATGTCCGACGGAGCTTTAACGGTCCTCGACGGCACTCACCTGAGAGCCGTCGACCTCTCTCTTCCCGACTCTGACGTCATCCTGACCGGAGGTCACGTGCTCGACCTCGCCGACTCCAAAGCCTCTTCGTCTCTTTTCGGCCTGTCCTTGCCTCAGGGACTCAAGTCCTCTGCTTTGAAACGCGTCAATGTCCAAGACGACGACGTTTTCCGTCAAGCCGAGCTCGATCGCCACCAGGCTCTGAAGCTCGCCGGCGACTACATCGCCGCGATCGCCGATGAGTTGAAAGATGATCCGCTTGTCATATCTATCTTGGATGGAAACACGATTCGGCTGTTTTTGGAGGATGAGGATGACTTTGCAATGCTAGCGGAGAATCTGTTCACTGATCTTGATGCAGAAGATGTAGGAAAGATCAACAAGAGCGCGATCCGGAGTGCGCTGGTTCACATGGGAGTTGAGATGGGTGTCCCTCCCGCTTCAGAATTTCCTCCACTAAGTGATATCTTGACGAAACATGAAGCTAATGGGGAAGAAGAACTAGGCCAAGCACAATTTGCTGAATTACTCCAGCCTATCCTACAAGAACTTGCAGAGGCCCTGGCAAAAAAGAATGTTGTTTTTATACAGAACATCAAGATTGTGAATGGTTCTAAGCTGAGAAAGGTAGTTCCTTTTTCACTGCTCATATATTATTTCTTCTTTTTTGACAAACTTTTGTGTATCTATACCTCTGGCTGTTATATTCGAAAAAGGGTACTCTTGAATTTTCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5 EF-hand_7

Protein Analysis

258

Amino Acids

28.42

Weight (kDa)

4.73

Isoelectric Point (pI)

36.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014521)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G28830
fragaria_vesca FvH4_6g02990
malus_domestica MD04G1224000.v1.1 MD12G1240800.v1.1
prunus_persica Prupe.6G342700_v2.0.a1
pyrus_communis pycom04g19740 pycom12g22050
rosa_chinensis RchiOBHm_Chr3g0451171
rosa_laevigata RLG00000025680
rosa_multiflora Rmu_co8512575.1_g000001
rosa_roxburghii Rroxscaffold_6G00428640
rosa_rugosa Rorug02G0632500
rosa_samantha Rh3AG035000 Rh3BG035400 Rh3CG034000 Rh3DG035000
rosa_wichuraiana Rw3G002570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 74
AatII GACGTC 1 cut(s) 78
AccI GTMKAC 1 cut(s) 51
AccII CGCG 3 cut(s) 183, 269, 428
AccIII TCCGGA 1 cut(s) 432
AciI CCGC 4 cut(s) 267, 296, 371, 477
AclWI GGATC 2 cut(s) 287, 424
AcsI RAATTY 2 cut(s) 485, 766
AcuI CTGAAG 2 cut(s) 263, 465
AcvI CACGTG 1 cut(s) 97
AcyI GRCGYC 1 cut(s) 75
AdeI CACNNNGTG 1 cut(s) 500
AfaI GTAC 1 cut(s) 759
AflIII ACRYGT 1 cut(s) 181
AgsI TTSAA 3 cut(s) 178, 286, 766
AhdI GACNNNNNGTC 1 cut(s) 90
AjnI CCWGG 2 cut(s) 234, 600
AloI GAACNNNNNNTCC 2 cut(s) 365, 397
AluBI AGCT 5 cut(s) 14, 223, 247, 524, 658
AluI AGCT 5 cut(s) 14, 223, 247, 524, 658
Alw21I GWGCWC 2 cut(s) 102, 225
Alw26I GTCTC 1 cut(s) 135
AlwI GGATC 2 cut(s) 287, 424
AlwNI CAGNNNCTG 1 cut(s) 241
Aor13HI TCCGGA 1 cut(s) 432
AoxI GGCC 3 cut(s) 139, 544, 597
ApoI RAATTY 2 cut(s) 485, 766
AsiSI GCGATCGC 1 cut(s) 273
Asp700I GAANNNNTTC 1 cut(s) 380
AspLEI GCGC 2 cut(s) 428, 442
AspS9I GGNCC 2 cut(s) 22, 598
AsuHPI GGTGA 1 cut(s) 29
AsuII TTCGAA 1 cut(s) 748
AsuNHI GCTAGC 1 cut(s) 367
AvaII GGWCC 1 cut(s) 22
AxyI CCTNAGG 1 cut(s) 153
BanII GRGCYC 1 cut(s) 225
BbrPI CACGTG 1 cut(s) 97
Bbv12I GWGCWC 2 cut(s) 102, 225
BccI CCATC 2 cut(s) 311, 457
BceAI ACGGC 2 cut(s) 32, 46
BciT130I CCWGG 2 cut(s) 236, 602
BcoDI GTCTC 1 cut(s) 135
BfaI CTAG 2 cut(s) 368, 542
BisI GCNGC 1 cut(s) 267
BlsI GCNGC 1 cut(s) 268
Bme1390I CCNGG 2 cut(s) 236, 602
Bme18I GGWCC 1 cut(s) 22
BmeRI GACNNNNNGTC 1 cut(s) 90
BmgT120I GGNCC 2 cut(s) 22, 598
BmrFI CCNGG 2 cut(s) 236, 602
BmsI GCATC 1 cut(s) 385
BmtI GCTAGC 1 cut(s) 371
BoxI GACNNNNGTC 1 cut(s) 163
BpmI CTGGAG 1 cut(s) 554
Bpu14I TTCGAA 1 cut(s) 748
BpuEI CTTGAG 1 cut(s) 146
BsaAI YACGTR 1 cut(s) 97
BsaHI GRCGYC 1 cut(s) 75
BsaJI CCNNGG 1 cut(s) 600
BsaWI WCCGGW 2 cut(s) 86, 432
BsaXI ACNNNNNCTCC 4 cut(s) 81, 111, 365, 395
Bse118I RCCGGY 1 cut(s) 251
Bse21I CCTNAGG 1 cut(s) 153
Bse3DI GCAATG 1 cut(s) 369
BseAI TCCGGA 1 cut(s) 432
BseBI CCWGG 2 cut(s) 236, 602
BseDI CCNNGG 1 cut(s) 600
BseGI GGATG 4 cut(s) 78, 322, 352, 358
BseMI GCAATG 1 cut(s) 369
BseMII CTCAG 3 cut(s) 32, 167, 650
Bsh1236I CGCG 3 cut(s) 183, 269, 428
Bsh1285I CGRYCG 2 cut(s) 229, 273
BshFI GGCC 3 cut(s) 141, 546, 599
BsiEI CGRYCG 2 cut(s) 229, 273
BsiHKAI GWGCWC 2 cut(s) 102, 225
BsiSI CCGG 3 cut(s) 87, 252, 433
BslFI GGGAC 2 cut(s) 171, 455
BsmAI GTCTC 1 cut(s) 135
BsmBI CGTCTC 1 cut(s) 135
BsmFI GGGAC 2 cut(s) 171, 455
BsnI GGCC 3 cut(s) 141, 546, 599
Bsp119I TTCGAA 1 cut(s) 748
Bsp1286I GDGCHC 2 cut(s) 102, 225
Bsp13I TCCGGA 1 cut(s) 432
Bsp143I GATC 6 cut(s) 226, 270, 292, 388, 414, 429
BspACI CCGC 4 cut(s) 267, 296, 371, 477
BspANI GGCC 3 cut(s) 141, 546, 599
BspCNI CTCAG 3 cut(s) 33, 166, 651
BspEI TCCGGA 1 cut(s) 432
BspFNI CGCG 3 cut(s) 183, 269, 428
BspOI GCTAGC 1 cut(s) 371
BspPI GGATC 2 cut(s) 287, 424
BspT104I TTCGAA 1 cut(s) 748
BsrDI GCAATG 1 cut(s) 369
BsrFI RCCGGY 1 cut(s) 251
BssAI RCCGGY 1 cut(s) 251
BssECI CCNNGG 1 cut(s) 600
BssMI GATC 6 cut(s) 226, 270, 292, 388, 414, 429
BssNI GRCGYC 1 cut(s) 75
Bst2UI CCWGG 2 cut(s) 236, 602
Bst4CI ACNGT 1 cut(s) 22
Bst6I CTCTTC 2 cut(s) 66, 130
BstACI GRCGYC 1 cut(s) 75
BstAPI GCANNNNNTGC 1 cut(s) 557
BstBAI YACGTR 1 cut(s) 97
BstBI TTCGAA 1 cut(s) 748
BstC8I GCNNGC 3 cut(s) 249, 253, 369
BstDEI CTNAG 5 cut(s) 41, 153, 497, 654, 659
BstF5I GGATG 4 cut(s) 78, 322, 352, 358
BstFNI CGCG 3 cut(s) 183, 269, 428
BstHHI GCGC 2 cut(s) 428, 442
BstKTI GATC 6 cut(s) 229, 273, 295, 391, 417, 432
BstMAI GTCTC 1 cut(s) 135
BstMBI GATC 6 cut(s) 226, 270, 292, 388, 414, 429
BstMCI CGRYCG 2 cut(s) 229, 273
BstMWI GCNNNNNNNGC 3 cut(s) 272, 368, 557
BstNI CCWGG 2 cut(s) 236, 602
BstPAI GACNNNNGTC 1 cut(s) 163
BstSCI CCNGG 2 cut(s) 234, 600
BstUI CGCG 3 cut(s) 183, 269, 428
Bsu36I CCTNAGG 1 cut(s) 153
BsuRI GGCC 3 cut(s) 141, 546, 599
BtgZI GCGATG 1 cut(s) 247
BtsCI GGATG 4 cut(s) 78, 322, 352, 358
BtsI GCAGTG 1 cut(s) 680
BtsIMutI CAGTG 2 cut(s) 384, 680
Cac8I GCNNGC 3 cut(s) 249, 253, 369
CaiI CAGNNNCTG 1 cut(s) 241
CfoI GCGC 2 cut(s) 428, 442
Cfr10I RCCGGY 1 cut(s) 251
Cfr13I GGNCC 2 cut(s) 22, 598
CseI GACGC 1 cut(s) 172
Csp6I GTAC 1 cut(s) 758
CviAII CATG 2 cut(s) 451, 518
CviQI GTAC 1 cut(s) 758
DdeI CTNAG 5 cut(s) 41, 153, 497, 654, 659
DpnI GATC 6 cut(s) 228, 272, 294, 390, 416, 431
DpnII GATC 6 cut(s) 226, 270, 292, 388, 414, 429
DraIII CACNNNGTG 1 cut(s) 500
DrdI GACNNNNNNGTC 1 cut(s) 74
DriI GACNNNNNGTC 1 cut(s) 90
DseDI GACNNNNNNGTC 1 cut(s) 74
Eam1104I CTCTTC 2 cut(s) 66, 130
Eam1105I GACNNNNNGTC 1 cut(s) 90
EarI CTCTTC 2 cut(s) 66, 130
Ecl136II GAGCTC 1 cut(s) 223
Eco24I GRGCYC 1 cut(s) 225
Eco32I GATATC 1 cut(s) 505
Eco47I GGWCC 1 cut(s) 22
Eco53kI GAGCTC 1 cut(s) 223
Eco57I CTGAAG 2 cut(s) 263, 465
Eco72I CACGTG 1 cut(s) 97
Eco81I CCTNAGG 1 cut(s) 153
EcoICRI GAGCTC 1 cut(s) 223
EcoO109I RGGNCCY 1 cut(s) 598
EcoRII CCWGG 2 cut(s) 234, 600
EcoRV GATATC 1 cut(s) 505
EcoT38I GRGCYC 1 cut(s) 225
Esp3I CGTCTC 1 cut(s) 135
FaeI CATG 2 cut(s) 454, 521
FaiI YATR 8 cut(s) 305, 452, 519, 626, 689, 691, 729, 744
FaqI GGGAC 2 cut(s) 171, 455
FatI CATG 2 cut(s) 450, 517
FauI CCCGC 1 cut(s) 484
FblI GTMKAC 1 cut(s) 51
Fnu4HI GCNGC 1 cut(s) 267
FokI GGATG 4 cut(s) 65, 329, 359, 365
FriOI GRGCYC 1 cut(s) 225
Fsp4HI GCNGC 1 cut(s) 267
FspBI CTAG 2 cut(s) 368, 542
GlaI GCGC 2 cut(s) 427, 441
GluI GCNGC 1 cut(s) 267
GsuI CTGGAG 1 cut(s) 554
HaeIII GGCC 3 cut(s) 141, 546, 599
HapII CCGG 3 cut(s) 87, 252, 433
HgaI GACGC 1 cut(s) 172
HhaI GCGC 2 cut(s) 428, 442
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 2 cut(s) 454, 521
Hin6I GCGC 2 cut(s) 426, 440
HinP1I GCGC 2 cut(s) 426, 440
HincII GTYRAC 1 cut(s) 52
HindII GTYRAC 1 cut(s) 52
HinfI GANTC 5 cut(s) 68, 113, 159, 328, 376
HpaII CCGG 3 cut(s) 87, 252, 433
HphI GGTGA 1 cut(s) 29
Hpy166II GTNNAC 3 cut(s) 52, 384, 448
Hpy188I TCNGA 4 cut(s) 7, 73, 243, 484
Hpy188III TCNNGA 7 cut(s) 65, 82, 392, 433, 508, 637, 763
Hpy8I GTNNAC 3 cut(s) 52, 384, 448
Hpy99I CGWCG 5 cut(s) 11, 32, 53, 203, 206
HpyCH4III ACNGT 1 cut(s) 22
HpyCH4IV ACGT 3 cut(s) 75, 96, 204
HpyCH4V TGCA 3 cut(s) 362, 398, 593
HpyF10VI GCNNNNNNNGC 3 cut(s) 272, 368, 557
HpyF3I CTNAG 5 cut(s) 41, 153, 497, 654, 659
HpySE526I ACGT 3 cut(s) 75, 96, 204
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 2 cut(s) 454, 521
HspAI GCGC 2 cut(s) 426, 440
Kpn2I TCCGGA 1 cut(s) 432
KroI GCCGGC 1 cut(s) 251
KroNI GCCGGC 1 cut(s) 253
Kzo9I GATC 6 cut(s) 226, 270, 292, 388, 414, 429
LmnI GCTCC 1 cut(s) 11
LweI GCATC 1 cut(s) 385
MaeI CTAG 2 cut(s) 368, 542
MaeII ACGT 3 cut(s) 75, 96, 204
MaeIII GTNAC 1 cut(s) 92
MalI GATC 6 cut(s) 228, 272, 294, 390, 416, 431
MboI GATC 6 cut(s) 226, 270, 292, 388, 414, 429
MboII GAAGA 6 cut(s) 53, 117, 413, 545, 548, 691
MhlI GDGCHC 2 cut(s) 102, 225
MluCI AATT 4 cut(s) 485, 554, 563, 766
MluI ACGCGT 1 cut(s) 181
MlyI GAGTC 3 cut(s) 62, 107, 153
MmeI TCCRAC 1 cut(s) 30
MreI CGCCGGCG 1 cut(s) 251
MroI TCCGGA 1 cut(s) 432
MroNI GCCGGC 1 cut(s) 251
MroXI GAANNNNTTC 1 cut(s) 380
MseI TTAA 2 cut(s) 17, 775
MspI CCGG 3 cut(s) 87, 252, 433
MspR9I CCNGG 2 cut(s) 236, 602
MvaI CCWGG 2 cut(s) 236, 602
MvnI CGCG 3 cut(s) 183, 269, 428
MwoI GCNNNNNNNGC 3 cut(s) 272, 368, 557
NaeI GCCGGC 1 cut(s) 253
NdeII GATC 6 cut(s) 226, 270, 292, 388, 414, 429
NgoMIV GCCGGC 1 cut(s) 251
NheI GCTAGC 1 cut(s) 367
NlaIII CATG 2 cut(s) 454, 521
NmeAIII GCCGAG 1 cut(s) 244
NmuCI GTSAC 1 cut(s) 92
NspV TTCGAA 1 cut(s) 748
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PdiI GCCGGC 1 cut(s) 253
PdmI GAANNNNTTC 1 cut(s) 380
PfeI GAWTC 2 cut(s) 328, 376
PkrI GCNGC 1 cut(s) 268
Ple19I CGATCG 2 cut(s) 229, 273
PleI GAGTC 3 cut(s) 62, 107, 153
PmaCI CACGTG 1 cut(s) 97
PmlI CACGTG 1 cut(s) 97
PpsI GAGTC 3 cut(s) 62, 107, 153
Ppu21I YACGTR 1 cut(s) 97
PshAI GACNNNNGTC 1 cut(s) 163
Psp124BI GAGCTC 1 cut(s) 225
Psp6I CCWGG 2 cut(s) 234, 600
PspCI CACGTG 1 cut(s) 97
PspGI CCWGG 2 cut(s) 234, 600
PspPI GGNCC 2 cut(s) 22, 598
PstNI CAGNNNCTG 1 cut(s) 241
PvuI CGATCG 2 cut(s) 229, 273
RgaI GCGATCGC 1 cut(s) 273
RsaI GTAC 1 cut(s) 759
RsaNI GTAC 1 cut(s) 758
SacI GAGCTC 1 cut(s) 225
SalI GTCGAC 1 cut(s) 50
SaqAI TTAA 2 cut(s) 17, 775
SatI GCNGC 1 cut(s) 267
Sau3AI GATC 6 cut(s) 226, 270, 292, 388, 414, 429
Sau96I GGNCC 2 cut(s) 22, 598
SchI GAGTC 3 cut(s) 62, 107, 153
ScrFI CCNGG 2 cut(s) 236, 602
SduI GDGCHC 2 cut(s) 102, 225
SfaAI GCGATCGC 1 cut(s) 273
SfaNI GCATC 1 cut(s) 385
SfuI TTCGAA 1 cut(s) 748
SgfI GCGATCGC 1 cut(s) 273
SgrAI CRCCGGYG 1 cut(s) 251
SinI GGWCC 1 cut(s) 22
SmlI CTYRAG 1 cut(s) 161
SmoI CTYRAG 1 cut(s) 161
Sse9I AATT 4 cut(s) 485, 554, 563, 766
SsiI CCGC 4 cut(s) 267, 296, 371, 477
SspMI CTAG 2 cut(s) 368, 542
SstI GAGCTC 1 cut(s) 225
StyD4I CCNGG 2 cut(s) 234, 600
TaaI ACNGT 1 cut(s) 22
TaiI ACGT 3 cut(s) 78, 99, 207
TaqI TCGA 5 cut(s) 27, 51, 102, 225, 748
TasI AATT 4 cut(s) 485, 554, 563, 766
TauI GCSGC 1 cut(s) 269
TfiI GAWTC 2 cut(s) 328, 376
Tru1I TTAA 2 cut(s) 17, 775
Tru9I TTAA 2 cut(s) 17, 775
TscAI CASTG 2 cut(s) 391, 687
TseFI GTSAC 1 cut(s) 92
Tsp45I GTSAC 1 cut(s) 92
TspDTI ATGAA 2 cut(s) 534, 761
TspGWI ACGGA 2 cut(s) 24, 200
TspRI CASTG 2 cut(s) 391, 687
VpaK11BI GGWCC 1 cut(s) 22
XapI RAATTY 2 cut(s) 485, 766
XmiI GTMKAC 1 cut(s) 51
XmnI GAANNNNTTC 1 cut(s) 380
XspI CTAG 2 cut(s) 368, 542
ZraI GACGTC 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.