Prupe.6G342700_v2.0.a1

Calcium-binding EF hand family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0014521
Physical Location & Seq
Forward (+)
29526650 .. 29529832
3183 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G342700.1

Sequence Viewer

Length: 942 bp
ATGTCGGACGGAGCTTTAACGGTCCTCGACGGGAACCACCTGAGAGCCATCGACCTTTCTCTGCCGGAAGCTGAGGTCAGCCTCACTGGAGCTCAGGTCCTCGACCTCGCCGACTCCAAAGCCTCCTCGTCTCTCTTCGGCCTCTCCTTGCCTCAGAGCCTCAAGTCCTCTGCTCTGAAACGCATCAGCCTTCAAGACGACGACGTCTTCCGCCTCAAGGAGCTAGATCGCGAGCAGGCTCTGAAGGTGATCACGGACTACATCACAGCGATCGCCGATGAATTAAAAGATGATCCACTTGTCATATCTGTCTTGGATGGATACACTCTTCGACTGTTTTTAGAGGATGAGGATGATTTTGCAATGCTAGCAGAGAATCTGTTCACTGATCTGGATGTAGAAGACACAGGAAAGATCAACAAGAATGAGATCCGGAATGCACTTGTTCACATGGGAGTTGAGATGGGCGTTCCTCCCATTTCAGAATTTCCTCCTCTAAGTGATATCTTGAAGAAACACAAAGCTGATGGGGAAGAAGAACTGGGCCAAGCACAATTTGCTGAATTACTCCAGCCTGTCCTACAAGAACTATCAGAGGCCCTGGCAAAAAAGCATTTTGTTACTATCCAGAACATCAAGATTGTCAATGGTTCCAAGCTAAGAAAGCTTTTGGCAGATGAGAAGCAATTGAACATTATCGTAGAGAAGATATTGGCGGATGGCTCGGGGAACACAGAAAAAATCAGAAGTTTTCTTGAAAAAACTGGGACAGAGTTGGGCTTGCCACCATCCGAAGCAAACGAGGCAGTGGCCCTTTTGTATGATGCAGTGTTTGCTGATTTAGAGGAAGCAGGTGAGGATAAATTTGGAAATCTTGTGAAGCAGATCCTGGAGAAATTTGCAGAGCAGCTAGAAGCAAGCCCTGTTTTTCATGATATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5 EF-hand_7

Protein Analysis

314

Amino Acids

34.39

Weight (kDa)

4.46

Isoelectric Point (pI)

40.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014521)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G28830
fragaria_vesca FvH4_6g02990
malus_domestica MD04G1224000.v1.1 MD12G1240800.v1.1
prunus_persica Prupe.6G342700_v2.0.a1
pyrus_communis pycom04g19740 pycom12g22050
rosa_chinensis RchiOBHm_Chr3g0451171
rosa_laevigata RLG00000025680
rosa_multiflora Rmu_co8512575.1_g000001
rosa_roxburghii Rroxscaffold_6G00428640
rosa_rugosa Rorug02G0632500
rosa_samantha Rh3AG035000 Rh3BG035400 Rh3CG034000 Rh3DG035000
rosa_wichuraiana Rw3G002570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 842
AasI GACNNNNNNGTC 1 cut(s) 203
AatII GACGTC 1 cut(s) 207
Acc36I ACCTGC 1 cut(s) 842
AccII CGCG 1 cut(s) 231
AccIII TCCGGA 1 cut(s) 432
AciI CCGC 2 cut(s) 211, 716
AclWI GGATC 3 cut(s) 287, 424, 880
AcsI RAATTY 3 cut(s) 485, 863, 896
AcuI CTGAAG 1 cut(s) 263
AcyI GRCGYC 1 cut(s) 204
AfiI CCNNNNNNNGG 1 cut(s) 217
AgsI TTSAA 4 cut(s) 194, 511, 691, 758
AjnI CCWGG 2 cut(s) 600, 888
AluBI AGCT 8 cut(s) 14, 71, 92, 223, 524, 658, 667, 910
AluI AGCT 8 cut(s) 14, 71, 92, 223, 524, 658, 667, 910
Alw21I GWGCWC 1 cut(s) 94
Alw26I GTCTC 1 cut(s) 135
AlwI GGATC 3 cut(s) 287, 424, 880
AlwNI CAGNNNCTG 2 cut(s) 241, 889
Ama87I CYCGRG 1 cut(s) 724
Aor13HI TCCGGA 1 cut(s) 432
AoxI GGCC 4 cut(s) 139, 544, 597, 810
ApeKI GCWGC 1 cut(s) 907
ApoI RAATTY 3 cut(s) 485, 863, 896
AsiSI GCGATCGC 1 cut(s) 273
Asp700I GAANNNNTTC 1 cut(s) 380
AspS9I GGNCC 5 cut(s) 22, 97, 544, 598, 811
AsuHPI GGTGA 2 cut(s) 259, 866
AsuNHI GCTAGC 1 cut(s) 367
AvaI CYCGRG 1 cut(s) 724
AvaII GGWCC 2 cut(s) 22, 97
BanII GRGCYC 1 cut(s) 94
BbsI GAAGAC 2 cut(s) 199, 408
Bbv12I GWGCWC 1 cut(s) 94
BbvCI CCTCAGC 1 cut(s) 72
BbvI GCAGC 1 cut(s) 919
BccI CCATC 6 cut(s) 56, 311, 457, 521, 713, 796
BciT130I CCWGG 2 cut(s) 602, 890
BciVI GTATCC 1 cut(s) 314
BclI TGATCA 1 cut(s) 249
BcoDI GTCTC 1 cut(s) 135
BfaI CTAG 3 cut(s) 224, 368, 911
BfuAI ACCTGC 1 cut(s) 842
BfuI GTATCC 1 cut(s) 314
BisI GCNGC 1 cut(s) 908
BlsI GCNGC 1 cut(s) 909
Bme1390I CCNGG 2 cut(s) 602, 890
Bme18I GGWCC 2 cut(s) 22, 97
BmeT110I CYCGRG 1 cut(s) 724
BmgT120I GGNCC 5 cut(s) 22, 97, 544, 598, 811
BmiI GGNNCC 2 cut(s) 35, 652
BmrFI CCNGG 2 cut(s) 602, 890
BmrI ACTGGG 2 cut(s) 551, 774
BmsI GCATC 2 cut(s) 192, 814
BmtI GCTAGC 1 cut(s) 371
BmuI ACTGGG 2 cut(s) 551, 774
BpiI GAAGAC 2 cut(s) 199, 408
BpmI CTGGAG 3 cut(s) 108, 554, 911
Bpu10I CCTNAGC 2 cut(s) 72, 93
BpuEI CTTGAG 2 cut(s) 146, 200
BsaHI GRCGYC 1 cut(s) 204
BsaJI CCNNGG 1 cut(s) 600
BsaWI WCCGGW 1 cut(s) 432
BsaXI ACNNNNNCTCC 2 cut(s) 81, 111
Bsc4I CCNNNNNNNGG 1 cut(s) 217
Bse1I ACTGG 3 cut(s) 91, 546, 769
Bse3DI GCAATG 1 cut(s) 369
BseAI TCCGGA 1 cut(s) 432
BseBI CCWGG 2 cut(s) 602, 890
BseDI CCNNGG 1 cut(s) 600
BseGI GGATG 6 cut(s) 322, 352, 358, 400, 724, 788
BseLI CCNNNNNNNGG 1 cut(s) 217
BseMI GCAATG 1 cut(s) 369
BseMII CTCAG 4 cut(s) 32, 63, 107, 167
BseNI ACTGG 3 cut(s) 91, 546, 769
BseRI GAGGAG 2 cut(s) 115, 483
BseXI GCAGC 1 cut(s) 919
Bsh1236I CGCG 1 cut(s) 231
Bsh1285I CGRYCG 1 cut(s) 273
BshFI GGCC 4 cut(s) 141, 546, 599, 812
BsiEI CGRYCG 1 cut(s) 273
BsiHKAI GWGCWC 1 cut(s) 94
BsiHKCI CYCGRG 1 cut(s) 724
BsiSI CCGG 2 cut(s) 65, 433
BslFI GGGAC 1 cut(s) 781
BslI CCNNNNNNNGG 1 cut(s) 217
BsmAI GTCTC 1 cut(s) 135
BsmBI CGTCTC 1 cut(s) 135
BsmFI GGGAC 1 cut(s) 781
BsmI GAATGC 1 cut(s) 442
BsnI GGCC 4 cut(s) 141, 546, 599, 812
BsoBI CYCGRG 1 cut(s) 724
Bsp1286I GDGCHC 1 cut(s) 94
Bsp13I TCCGGA 1 cut(s) 432
Bsp143I GATC 8 cut(s) 226, 249, 270, 292, 388, 414, 429, 885
Bsp68I TCGCGA 1 cut(s) 231
BspACI CCGC 2 cut(s) 211, 716
BspANI GGCC 4 cut(s) 141, 546, 599, 812
BspCNI CTCAG 4 cut(s) 33, 64, 106, 166
BspEI TCCGGA 1 cut(s) 432
BspFNI CGCG 1 cut(s) 231
BspHI TCATGA 1 cut(s) 931
BspLI GGNNCC 2 cut(s) 35, 652
BspMI ACCTGC 1 cut(s) 842
BspOI GCTAGC 1 cut(s) 371
BspPI GGATC 3 cut(s) 287, 424, 880
BsrDI GCAATG 1 cut(s) 369
BsrI ACTGG 3 cut(s) 91, 546, 769
BssECI CCNNGG 1 cut(s) 600
BssMI GATC 8 cut(s) 226, 249, 270, 292, 388, 414, 429, 885
BssNI GRCGYC 1 cut(s) 204
Bst2UI CCWGG 2 cut(s) 602, 890
Bst4CI ACNGT 2 cut(s) 22, 336
Bst6I CTCTTC 2 cut(s) 140, 333
BstACI GRCGYC 1 cut(s) 204
BstAPI GCANNNNNTGC 2 cut(s) 557, 833
BstC8I GCNNGC 5 cut(s) 233, 237, 369, 782, 919
BstDEI CTNAG 6 cut(s) 41, 72, 93, 153, 497, 659
BstF5I GGATG 6 cut(s) 322, 352, 358, 400, 724, 788
BstFNI CGCG 1 cut(s) 231
BstKTI GATC 8 cut(s) 229, 252, 273, 295, 391, 417, 432, 888
BstMAI GTCTC 1 cut(s) 135
BstMBI GATC 8 cut(s) 226, 249, 270, 292, 388, 414, 429, 885
BstMCI CGRYCG 1 cut(s) 273
BstMWI GCNNNNNNNGC 5 cut(s) 368, 557, 664, 803, 833
BstNI CCWGG 2 cut(s) 602, 890
BstSCI CCNGG 2 cut(s) 600, 888
BstUI CGCG 1 cut(s) 231
BstV1I GCAGC 1 cut(s) 919
BstV2I GAAGAC 2 cut(s) 199, 408
BstX2I RGATCY 2 cut(s) 429, 885
BstYI RGATCY 2 cut(s) 429, 885
BsuI GTATCC 1 cut(s) 314
BsuRI GGCC 4 cut(s) 141, 546, 599, 812
BtsCI GGATG 6 cut(s) 322, 352, 358, 400, 724, 788
BtsI GCAGTG 2 cut(s) 813, 834
BtsIMutI CAGTG 4 cut(s) 84, 384, 813, 834
BtuMI TCGCGA 1 cut(s) 231
BveI ACCTGC 1 cut(s) 842
Cac8I GCNNGC 5 cut(s) 233, 237, 369, 782, 919
CaiI CAGNNNCTG 2 cut(s) 241, 889
CciI TCATGA 1 cut(s) 931
Cfr13I GGNCC 5 cut(s) 22, 97, 544, 598, 811
CviAII CATG 2 cut(s) 451, 932
DdeI CTNAG 6 cut(s) 41, 72, 93, 153, 497, 659
DpnI GATC 8 cut(s) 228, 251, 272, 294, 390, 416, 431, 887
DpnII GATC 8 cut(s) 226, 249, 270, 292, 388, 414, 429, 885
DrdI GACNNNNNNGTC 1 cut(s) 203
DseDI GACNNNNNNGTC 1 cut(s) 203
Eam1104I CTCTTC 2 cut(s) 140, 333
EarI CTCTTC 2 cut(s) 140, 333
EciI GGCGGA 2 cut(s) 200, 731
Ecl136II GAGCTC 1 cut(s) 92
Eco24I GRGCYC 1 cut(s) 94
Eco32I GATATC 1 cut(s) 505
Eco47I GGWCC 2 cut(s) 22, 97
Eco53kI GAGCTC 1 cut(s) 92
Eco57I CTGAAG 1 cut(s) 263
Eco88I CYCGRG 1 cut(s) 724
EcoICRI GAGCTC 1 cut(s) 92
EcoO109I RGGNCCY 2 cut(s) 97, 598
EcoRII CCWGG 2 cut(s) 600, 888
EcoRV GATATC 1 cut(s) 505
EcoT38I GRGCYC 1 cut(s) 94
Esp3I CGTCTC 1 cut(s) 135
FaeI CATG 2 cut(s) 454, 935
FaiI YATR 4 cut(s) 305, 452, 822, 933
FaqI GGGAC 1 cut(s) 781
FatI CATG 2 cut(s) 450, 931
FbaI TGATCA 1 cut(s) 249
Fnu4HI GCNGC 1 cut(s) 908
FokI GGATG 6 cut(s) 329, 359, 365, 407, 731, 775
FriOI GRGCYC 1 cut(s) 94
Fsp4HI GCNGC 1 cut(s) 908
FspBI CTAG 3 cut(s) 224, 368, 911
GluI GCNGC 1 cut(s) 908
GsuI CTGGAG 3 cut(s) 108, 554, 911
HaeIII GGCC 4 cut(s) 141, 546, 599, 812
HapII CCGG 2 cut(s) 65, 433
Hin1I GRCGYC 1 cut(s) 204
Hin1II CATG 2 cut(s) 454, 935
HindIII AAGCTT 1 cut(s) 665
HinfI GANTC 2 cut(s) 113, 376
HpaII CCGG 2 cut(s) 65, 433
HphI GGTGA 2 cut(s) 259, 866
Hpy166II GTNNAC 2 cut(s) 384, 448
Hpy188I TCNGA 8 cut(s) 7, 156, 177, 243, 484, 595, 746, 793
Hpy188III TCNNGA 9 cut(s) 194, 230, 392, 433, 508, 628, 637, 755, 932
Hpy8I GTNNAC 2 cut(s) 384, 448
Hpy99I CGWCG 3 cut(s) 32, 203, 206
HpyAV CCTTC 2 cut(s) 200, 238
HpyCH4III ACNGT 2 cut(s) 22, 336
HpyCH4IV ACGT 1 cut(s) 204
HpyCH4V TGCA 4 cut(s) 362, 440, 827, 902
HpyF10VI GCNNNNNNNGC 5 cut(s) 368, 557, 664, 803, 833
HpyF3I CTNAG 6 cut(s) 41, 72, 93, 153, 497, 659
HpySE526I ACGT 1 cut(s) 204
Hsp92I GRCGYC 1 cut(s) 204
Hsp92II CATG 2 cut(s) 454, 935
Kpn2I TCCGGA 1 cut(s) 432
Ksp22I TGATCA 1 cut(s) 249
Kzo9I GATC 8 cut(s) 226, 249, 270, 292, 388, 414, 429, 885
LmnI GCTCC 3 cut(s) 11, 89, 220
Lsp1109I GCAGC 1 cut(s) 919
LweI GCATC 2 cut(s) 192, 814
MaeI CTAG 3 cut(s) 224, 368, 911
MaeII ACGT 1 cut(s) 204
MaeIII GTNAC 1 cut(s) 619
MalI GATC 8 cut(s) 228, 251, 272, 294, 390, 416, 431, 887
MboI GATC 8 cut(s) 226, 249, 270, 292, 388, 414, 429, 885
MboII GAAGA 8 cut(s) 127, 199, 320, 413, 523, 545, 548, 718
MfeI CAATTG 1 cut(s) 686
MflI RGATCY 2 cut(s) 429, 885
MhlI GDGCHC 1 cut(s) 94
MluCI AATT 7 cut(s) 281, 485, 554, 563, 686, 863, 896
MlyI GAGTC 1 cut(s) 107
MroI TCCGGA 1 cut(s) 432
MroXI GAANNNNTTC 1 cut(s) 380
MseI TTAA 2 cut(s) 17, 284
MspI CCGG 2 cut(s) 65, 433
MspR9I CCNGG 2 cut(s) 602, 890
MunI CAATTG 1 cut(s) 686
Mva1269I GAATGC 1 cut(s) 442
MvaI CCWGG 2 cut(s) 602, 890
MvnI CGCG 1 cut(s) 231
MwoI GCNNNNNNNGC 5 cut(s) 368, 557, 664, 803, 833
NdeII GATC 8 cut(s) 226, 249, 270, 292, 388, 414, 429, 885
NheI GCTAGC 1 cut(s) 367
NlaIII CATG 2 cut(s) 454, 935
NlaIV GGNNCC 2 cut(s) 35, 652
NruI TCGCGA 1 cut(s) 231
PagI TCATGA 1 cut(s) 931
PaqCI CACCTGC 1 cut(s) 842
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PctI GAATGC 1 cut(s) 442
PdmI GAANNNNTTC 1 cut(s) 380
PfeI GAWTC 1 cut(s) 376
PflFI GACNNNGTC 1 cut(s) 203
PfoI TCCNGGA 1 cut(s) 888
PkrI GCNGC 1 cut(s) 909
Ple19I CGATCG 1 cut(s) 273
PleI GAGTC 1 cut(s) 107
PpsI GAGTC 1 cut(s) 107
PpuMI RGGWCCY 1 cut(s) 97
Psp124BI GAGCTC 1 cut(s) 94
Psp5II RGGWCCY 1 cut(s) 97
Psp6I CCWGG 2 cut(s) 600, 888
PspGI CCWGG 2 cut(s) 600, 888
PspN4I GGNNCC 2 cut(s) 35, 652
PspPI GGNCC 5 cut(s) 22, 97, 544, 598, 811
PspPPI RGGWCCY 1 cut(s) 97
PstNI CAGNNNCTG 2 cut(s) 241, 889
PsuI RGATCY 2 cut(s) 429, 885
PsyI GACNNNGTC 1 cut(s) 203
PvuI CGATCG 1 cut(s) 273
RgaI GCGATCGC 1 cut(s) 273
RruI TCGCGA 1 cut(s) 231
SacI GAGCTC 1 cut(s) 94
SaqAI TTAA 2 cut(s) 17, 284
SatI GCNGC 1 cut(s) 908
Sau3AI GATC 8 cut(s) 226, 249, 270, 292, 388, 414, 429, 885
Sau96I GGNCC 5 cut(s) 22, 97, 544, 598, 811
SchI GAGTC 1 cut(s) 107
ScrFI CCNGG 2 cut(s) 602, 890
SduI GDGCHC 1 cut(s) 94
SfaAI GCGATCGC 1 cut(s) 273
SfaNI GCATC 2 cut(s) 192, 814
SgfI GCGATCGC 1 cut(s) 273
SinI GGWCC 2 cut(s) 22, 97
SmlI CTYRAG 2 cut(s) 161, 215
SmoI CTYRAG 2 cut(s) 161, 215
Sse9I AATT 7 cut(s) 281, 485, 554, 563, 686, 863, 896
SsiI CCGC 2 cut(s) 211, 716
SspMI CTAG 3 cut(s) 224, 368, 911
SstI GAGCTC 1 cut(s) 94
StyD4I CCNGG 2 cut(s) 600, 888
TaaI ACNGT 2 cut(s) 22, 336
TaiI ACGT 1 cut(s) 207
TaqI TCGA 4 cut(s) 27, 51, 102, 331
TasI AATT 7 cut(s) 281, 485, 554, 563, 686, 863, 896
TfiI GAWTC 1 cut(s) 376
Tru1I TTAA 2 cut(s) 17, 284
Tru9I TTAA 2 cut(s) 17, 284
TscAI CASTG 4 cut(s) 91, 391, 813, 834
TseI GCWGC 1 cut(s) 907
TspDTI ATGAA 2 cut(s) 294, 920
TspGWI ACGGA 2 cut(s) 24, 269
TspRI CASTG 4 cut(s) 91, 391, 813, 834
Tth111I GACNNNGTC 1 cut(s) 203
VpaK11BI GGWCC 2 cut(s) 22, 97
XapI RAATTY 3 cut(s) 485, 863, 896
XmnI GAANNNNTTC 1 cut(s) 380
XspI CTAG 3 cut(s) 224, 368, 911
ZraI GACGTC 1 cut(s) 205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.