FvH4_6g02990

Calcium-binding EF hand family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0014521
Physical Location & Seq
Reverse (-)
1666070 .. 1670488
4419 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g02990.t1

Sequence Viewer

Length: 975 bp
ATGTCCGACGGAGCTTTAACGGTCCTTGACGGCAGTCACCTGAGAGCCGTGGACCTCTCTGTCCCCGACTCCGACGTCACCCTGACCGGAGCTCACGTCCTCGACCTCGCCGACTCCAAGGCCTCTTCGTCTCTCTTCGGCCTCACCTTGCCTCAGGGACTCAAGTCCTCTGCTCTGAAACGCGTCAATGTTCAAGACGACGACGTTTTCCGTCAAGCCGAGCTCGATCGCCACCAGGCTCTGAAGCTCGCCAGAGACTACATCGCCGCGATCGCCGATGAGTTGAAAGATGATCCGATTGTTATATCTATCTTGGATGGGAACACGATTCGGCTATTTTTGGAGGATGAGGATGACTTTGCAATGCTGGCAGAGAATCTGTTCACTGATCTTGATGCAGAAGATGTAGGAAAGCTCAGCAAGAGTGTGATCCAGAGCGCGCTGCTTCACATGGGAGTTGAGATGGGTGTCCCTCCCGTTTCAGAATTTCCTCCACTAAGTGATATATTGACGAAACATGGAGCTTATGGGGAAGAAGAACTTGGCCAAGCTCAATTCGCTGAATTACTCCAGCCTATCTTACAAGAACTAGCAGAAGCCCTGGCAAAAAAGAATGTTGTTCTTATCCAGAACATCAAGATTGTGAATGGTTCTAAGCTGAGAAAGCTGTTGGCTGATGAGAAACAAATCAACAGTGTCATAGAGAAGATATTGCAAGGGAAAAAGGATGGCTTGGGGAGTGCAGATGTAATCAGGAGTTATCTTGAGAAAAATGAGCTAGAGTTGGGGTTGCCACCATCTGAAGGAAACGAGCCAGCGGCTATTTTATATGATCTAGTGTTTACCGAGCTTGAGCAAGACAAGAGTGCTCTGAAAGAAGGCGAAGATAATTTCGGAAATCTTGTGAAGAAGATCCTTGAGAAACTGGCTGAGCAGCTAGAATCTAATCCTGTATTTCATGATACCGAGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5 EF-hand_7

Protein Analysis

325

Amino Acids

35.38

Weight (kDa)

4.51

Isoelectric Point (pI)

37.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014521)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G28830
fragaria_vesca FvH4_6g02990
malus_domestica MD04G1224000.v1.1 MD12G1240800.v1.1
prunus_persica Prupe.6G342700_v2.0.a1
pyrus_communis pycom04g19740 pycom12g22050
rosa_chinensis RchiOBHm_Chr3g0451171
rosa_laevigata RLG00000025680
rosa_multiflora Rmu_co8512575.1_g000001
rosa_roxburghii Rroxscaffold_6G00428640
rosa_rugosa Rorug02G0632500
rosa_samantha Rh3AG035000 Rh3BG035400 Rh3CG034000 Rh3DG035000
rosa_wichuraiana Rw3G002570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 59, 74
AatII GACGTC 1 cut(s) 78
AccII CGCG 3 cut(s) 183, 269, 440
AciI CCGC 2 cut(s) 267, 818
AclWI GGATC 3 cut(s) 287, 424, 907
AcoI YGGCCR 1 cut(s) 544
AcsI RAATTY 1 cut(s) 485
AcuI CTGAAG 2 cut(s) 263, 822
AcyI GRCGYC 1 cut(s) 75
AdeI CACNNNGTG 1 cut(s) 500
AfiI CCNNNNNNNGG 1 cut(s) 803
AflIII ACRYGT 1 cut(s) 181
AgsI TTSAA 2 cut(s) 194, 286
AjiI CACGTC 1 cut(s) 97
AjnI CCWGG 2 cut(s) 234, 600
AjuI GAANNNNNNNTTGG 2 cut(s) 525, 557
Alw21I GWGCWC 4 cut(s) 94, 225, 871, 972
Alw26I GTCTC 2 cut(s) 135, 249
AlwI GGATC 3 cut(s) 287, 424, 907
AlwNI CAGNNNCTG 1 cut(s) 241
AoxI GGCC 3 cut(s) 120, 139, 544
ApeKI GCWGC 2 cut(s) 442, 934
ApoI RAATTY 1 cut(s) 485
AsiSI GCGATCGC 1 cut(s) 273
Asp700I GAANNNNTTC 1 cut(s) 380
AspLEI GCGC 2 cut(s) 440, 442
AspS9I GGNCC 2 cut(s) 22, 52
AsuHPI GGTGA 3 cut(s) 29, 70, 136
AvaII GGWCC 2 cut(s) 22, 52
AxyI CCTNAGG 1 cut(s) 153
BalI TGGCCA 1 cut(s) 546
BanII GRGCYC 2 cut(s) 94, 225
Bbv12I GWGCWC 4 cut(s) 94, 225, 871, 972
BbvI GCAGC 2 cut(s) 429, 946
BccI CCATC 4 cut(s) 311, 457, 722, 805
BceAI ACGGC 2 cut(s) 32, 46
BciT130I CCWGG 2 cut(s) 236, 602
BcoDI GTCTC 2 cut(s) 135, 249
BfaI CTAG 4 cut(s) 590, 779, 836, 938
BisI GCNGC 4 cut(s) 267, 443, 819, 935
BlpI GCTNAGC 2 cut(s) 416, 930
BlsI GCNGC 4 cut(s) 268, 444, 820, 936
Bme1390I CCNGG 2 cut(s) 236, 602
Bme18I GGWCC 2 cut(s) 22, 52
BmgBI CACGTC 1 cut(s) 97
BmgT120I GGNCC 2 cut(s) 22, 52
BmrFI CCNGG 2 cut(s) 236, 602
BmsI GCATC 1 cut(s) 385
BoxI GACNNNNGTC 2 cut(s) 33, 163
BpmI CTGGAG 1 cut(s) 554
Bpu1102I GCTNAGC 2 cut(s) 416, 930
BpuEI CTTGAG 4 cut(s) 146, 785, 872, 938
BsaHI GRCGYC 1 cut(s) 75
BsaJI CCNNGG 3 cut(s) 48, 117, 600
BsaWI WCCGGW 1 cut(s) 86
BsaXI ACNNNNNCTCC 2 cut(s) 81, 111
Bsc4I CCNNNNNNNGG 1 cut(s) 803
Bse1I ACTGG 1 cut(s) 930
Bse21I CCTNAGG 1 cut(s) 153
Bse3DI GCAATG 1 cut(s) 369
BseBI CCWGG 2 cut(s) 236, 602
BseDI CCNNGG 3 cut(s) 48, 117, 600
BseGI GGATG 4 cut(s) 322, 352, 358, 733
BseLI CCNNNNNNNGG 1 cut(s) 803
BseMI GCAATG 1 cut(s) 369
BseMII CTCAG 5 cut(s) 32, 167, 430, 650, 921
BseNI ACTGG 1 cut(s) 930
BsePI GCGCGC 1 cut(s) 438
BseXI GCAGC 2 cut(s) 429, 946
BsgI GTGCAG 1 cut(s) 762
Bsh1236I CGCG 3 cut(s) 183, 269, 440
Bsh1285I CGRYCG 2 cut(s) 229, 273
BshFI GGCC 3 cut(s) 122, 141, 546
BsiEI CGRYCG 2 cut(s) 229, 273
BsiHKAI GWGCWC 4 cut(s) 94, 225, 871, 972
BsiSI CCGG 1 cut(s) 87
BslFI GGGAC 3 cut(s) 47, 171, 455
BslI CCNNNNNNNGG 1 cut(s) 803
BsmAI GTCTC 2 cut(s) 135, 249
BsmBI CGTCTC 1 cut(s) 135
BsmFI GGGAC 3 cut(s) 47, 171, 455
BsnI GGCC 3 cut(s) 122, 141, 546
Bsp1286I GDGCHC 4 cut(s) 94, 225, 871, 972
Bsp143I GATC 7 cut(s) 226, 270, 292, 388, 429, 832, 912
Bsp1720I GCTNAGC 2 cut(s) 416, 930
BspACI CCGC 2 cut(s) 267, 818
BspANI GGCC 3 cut(s) 122, 141, 546
BspCNI CTCAG 5 cut(s) 33, 166, 429, 651, 922
BspFNI CGCG 3 cut(s) 183, 269, 440
BspHI TCATGA 1 cut(s) 958
BspPI GGATC 3 cut(s) 287, 424, 907
BsrDI GCAATG 1 cut(s) 369
BsrI ACTGG 1 cut(s) 930
BssECI CCNNGG 3 cut(s) 48, 117, 600
BssHII GCGCGC 1 cut(s) 438
BssMI GATC 7 cut(s) 226, 270, 292, 388, 429, 832, 912
BssNI GRCGYC 1 cut(s) 75
BssT1I CCWWGG 1 cut(s) 117
Bst2UI CCWGG 2 cut(s) 236, 602
Bst4CI ACNGT 2 cut(s) 22, 695
Bst6I CTCTTC 2 cut(s) 130, 140
BstACI GRCGYC 1 cut(s) 75
BstC8I GCNNGC 4 cut(s) 249, 369, 440, 816
BstDEI CTNAG 7 cut(s) 41, 153, 416, 497, 654, 659, 930
BstDSI CCRYGG 1 cut(s) 48
BstF5I GGATG 4 cut(s) 322, 352, 358, 733
BstFNI CGCG 3 cut(s) 183, 269, 440
BstHHI GCGC 2 cut(s) 440, 442
BstKTI GATC 7 cut(s) 229, 273, 295, 391, 432, 835, 915
BstMAI GTCTC 2 cut(s) 135, 249
BstMBI GATC 7 cut(s) 226, 270, 292, 388, 429, 832, 912
BstMCI CGRYCG 2 cut(s) 229, 273
BstMWI GCNNNNNNNGC 4 cut(s) 272, 368, 557, 664
BstNI CCWGG 2 cut(s) 236, 602
BstPAI GACNNNNGTC 2 cut(s) 33, 163
BstSCI CCNGG 2 cut(s) 234, 600
BstUI CGCG 3 cut(s) 183, 269, 440
BstV1I GCAGC 2 cut(s) 429, 946
BstX2I RGATCY 1 cut(s) 912
BstYI RGATCY 1 cut(s) 912
Bsu36I CCTNAGG 1 cut(s) 153
BsuRI GGCC 3 cut(s) 122, 141, 546
BtgI CCRYGG 1 cut(s) 48
BtgZI GCGATG 1 cut(s) 247
BtrI CACGTC 1 cut(s) 97
BtsCI GGATG 4 cut(s) 322, 352, 358, 733
BtsIMutI CAGTG 3 cut(s) 384, 700, 970
Cac8I GCNNGC 4 cut(s) 249, 369, 440, 816
CaiI CAGNNNCTG 1 cut(s) 241
CciI TCATGA 1 cut(s) 958
CfoI GCGC 2 cut(s) 440, 442
Cfr13I GGNCC 2 cut(s) 22, 52
CseI GACGC 1 cut(s) 172
CviAII CATG 3 cut(s) 451, 518, 959
DdeI CTNAG 7 cut(s) 41, 153, 416, 497, 654, 659, 930
DpnI GATC 7 cut(s) 228, 272, 294, 390, 431, 834, 914
DpnII GATC 7 cut(s) 226, 270, 292, 388, 429, 832, 912
DraIII CACNNNGTG 1 cut(s) 500
DrdI GACNNNNNNGTC 2 cut(s) 59, 74
DseDI GACNNNNNNGTC 2 cut(s) 59, 74
EaeI YGGCCR 1 cut(s) 544
Eam1104I CTCTTC 2 cut(s) 130, 140
EarI CTCTTC 2 cut(s) 130, 140
Ecl136II GAGCTC 2 cut(s) 92, 223
Eco130I CCWWGG 1 cut(s) 117
Eco147I AGGCCT 1 cut(s) 122
Eco24I GRGCYC 2 cut(s) 94, 225
Eco47I GGWCC 2 cut(s) 22, 52
Eco53kI GAGCTC 2 cut(s) 92, 223
Eco57I CTGAAG 2 cut(s) 263, 822
Eco81I CCTNAGG 1 cut(s) 153
EcoICRI GAGCTC 2 cut(s) 92, 223
EcoRII CCWGG 2 cut(s) 234, 600
EcoT14I CCWWGG 1 cut(s) 117
EcoT38I GRGCYC 2 cut(s) 94, 225
ErhI CCWWGG 1 cut(s) 117
Esp3I CGTCTC 1 cut(s) 135
FaeI CATG 3 cut(s) 454, 521, 962
FaiI YATR 9 cut(s) 305, 452, 506, 519, 528, 701, 829, 831, 960
FalI AAGNNNNNCTT 4 cut(s) 525, 557, 716, 748
FaqI GGGAC 3 cut(s) 47, 171, 455
FatI CATG 3 cut(s) 450, 517, 958
Fnu4HI GCNGC 4 cut(s) 267, 443, 819, 935
FokI GGATG 4 cut(s) 329, 359, 365, 740
FriOI GRGCYC 2 cut(s) 94, 225
Fsp4HI GCNGC 4 cut(s) 267, 443, 819, 935
FspBI CTAG 4 cut(s) 590, 779, 836, 938
GlaI GCGC 2 cut(s) 439, 441
GluI GCNGC 4 cut(s) 267, 443, 819, 935
GsuI CTGGAG 1 cut(s) 554
HaeIII GGCC 3 cut(s) 122, 141, 546
HapII CCGG 1 cut(s) 87
HgaI GACGC 1 cut(s) 172
HhaI GCGC 2 cut(s) 440, 442
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 3 cut(s) 454, 521, 962
Hin6I GCGC 2 cut(s) 438, 440
HinP1I GCGC 2 cut(s) 438, 440
HinfI GANTC 6 cut(s) 68, 113, 159, 328, 376, 941
HpaII CCGG 1 cut(s) 87
HphI GGTGA 3 cut(s) 29, 70, 136
Hpy166II GTNNAC 3 cut(s) 52, 384, 843
Hpy188I TCNGA 9 cut(s) 7, 73, 177, 243, 297, 484, 802, 873, 896
Hpy188III TCNNGA 8 cut(s) 194, 392, 433, 628, 637, 754, 764, 959
Hpy8I GTNNAC 3 cut(s) 52, 384, 843
Hpy99I CGWCG 4 cut(s) 11, 77, 203, 206
HpyAV CCTTC 2 cut(s) 797, 872
HpyCH4III ACNGT 2 cut(s) 22, 695
HpyCH4IV ACGT 3 cut(s) 75, 96, 204
HpyCH4V TGCA 4 cut(s) 362, 398, 715, 743
HpyF10VI GCNNNNNNNGC 4 cut(s) 272, 368, 557, 664
HpyF3I CTNAG 7 cut(s) 41, 153, 416, 497, 654, 659, 930
HpySE526I ACGT 3 cut(s) 75, 96, 204
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 3 cut(s) 454, 521, 962
HspAI GCGC 2 cut(s) 438, 440
Kzo9I GATC 7 cut(s) 226, 270, 292, 388, 429, 832, 912
LmnI GCTCC 3 cut(s) 11, 89, 521
Lsp1109I GCAGC 2 cut(s) 429, 946
LweI GCATC 1 cut(s) 385
MaeI CTAG 4 cut(s) 590, 779, 836, 938
MaeII ACGT 3 cut(s) 75, 96, 204
MaeIII GTNAC 2 cut(s) 35, 76
MalI GATC 7 cut(s) 228, 272, 294, 390, 431, 834, 914
MboI GATC 7 cut(s) 226, 270, 292, 388, 429, 832, 912
MboII GAAGA 9 cut(s) 117, 127, 413, 545, 548, 718, 896, 919, 922
MflI RGATCY 1 cut(s) 912
MhlI GDGCHC 4 cut(s) 94, 225, 871, 972
MlsI TGGCCA 1 cut(s) 546
MluCI AATT 4 cut(s) 485, 554, 563, 889
MluI ACGCGT 1 cut(s) 181
MluNI TGGCCA 1 cut(s) 546
MlyI GAGTC 3 cut(s) 62, 107, 153
MmeI TCCRAC 2 cut(s) 30, 96
Mox20I TGGCCA 1 cut(s) 546
MroXI GAANNNNTTC 1 cut(s) 380
MscI TGGCCA 1 cut(s) 546
MseI TTAA 1 cut(s) 17
Msp20I TGGCCA 1 cut(s) 546
MspA1I CMGCKG 1 cut(s) 818
MspI CCGG 1 cut(s) 87
MspR9I CCNGG 2 cut(s) 236, 602
MvaI CCWGG 2 cut(s) 236, 602
MvnI CGCG 3 cut(s) 183, 269, 440
MwoI GCNNNNNNNGC 4 cut(s) 272, 368, 557, 664
NdeII GATC 7 cut(s) 226, 270, 292, 388, 429, 832, 912
NlaIII CATG 3 cut(s) 454, 521, 962
NmeAIII GCCGAG 1 cut(s) 244
NmuCI GTSAC 2 cut(s) 35, 76
PagI TCATGA 1 cut(s) 958
PauI GCGCGC 1 cut(s) 438
PceI AGGCCT 1 cut(s) 122
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PdmI GAANNNNTTC 1 cut(s) 380
PfeI GAWTC 3 cut(s) 328, 376, 941
PkrI GCNGC 4 cut(s) 268, 444, 820, 936
Ple19I CGATCG 2 cut(s) 229, 273
PleI GAGTC 3 cut(s) 62, 107, 153
PpsI GAGTC 3 cut(s) 62, 107, 153
PshAI GACNNNNGTC 2 cut(s) 33, 163
Psp124BI GAGCTC 2 cut(s) 94, 225
Psp6I CCWGG 2 cut(s) 234, 600
PspGI CCWGG 2 cut(s) 234, 600
PspPI GGNCC 2 cut(s) 22, 52
PstNI CAGNNNCTG 1 cut(s) 241
PsuI RGATCY 1 cut(s) 912
PteI GCGCGC 1 cut(s) 438
PvuI CGATCG 2 cut(s) 229, 273
RgaI GCGATCGC 1 cut(s) 273
SacI GAGCTC 2 cut(s) 94, 225
SaqAI TTAA 1 cut(s) 17
SatI GCNGC 4 cut(s) 267, 443, 819, 935
Sau3AI GATC 7 cut(s) 226, 270, 292, 388, 429, 832, 912
Sau96I GGNCC 2 cut(s) 22, 52
SchI GAGTC 3 cut(s) 62, 107, 153
ScrFI CCNGG 2 cut(s) 236, 602
SduI GDGCHC 4 cut(s) 94, 225, 871, 972
SfaAI GCGATCGC 1 cut(s) 273
SfaNI GCATC 1 cut(s) 385
SgfI GCGATCGC 1 cut(s) 273
SinI GGWCC 2 cut(s) 22, 52
SmlI CTYRAG 4 cut(s) 161, 764, 851, 917
SmoI CTYRAG 4 cut(s) 161, 764, 851, 917
Sse9I AATT 4 cut(s) 485, 554, 563, 889
SseBI AGGCCT 1 cut(s) 122
SsiI CCGC 2 cut(s) 267, 818
SspMI CTAG 4 cut(s) 590, 779, 836, 938
SstI GAGCTC 2 cut(s) 94, 225
StuI AGGCCT 1 cut(s) 122
StyD4I CCNGG 2 cut(s) 234, 600
StyI CCWWGG 1 cut(s) 117
TaaI ACNGT 2 cut(s) 22, 695
TaiI ACGT 3 cut(s) 78, 99, 207
TaqI TCGA 2 cut(s) 102, 225
TasI AATT 4 cut(s) 485, 554, 563, 889
TauI GCSGC 2 cut(s) 269, 821
TfiI GAWTC 3 cut(s) 328, 376, 941
Tru1I TTAA 1 cut(s) 17
Tru9I TTAA 1 cut(s) 17
TscAI CASTG 2 cut(s) 391, 700
TseFI GTSAC 2 cut(s) 35, 76
TseI GCWGC 2 cut(s) 442, 934
Tsp45I GTSAC 2 cut(s) 35, 76
TspDTI ATGAA 1 cut(s) 947
TspGWI ACGGA 2 cut(s) 24, 200
TspRI CASTG 2 cut(s) 391, 700
VpaK11BI GGWCC 2 cut(s) 22, 52
XapI RAATTY 1 cut(s) 485
XmnI GAANNNNTTC 1 cut(s) 380
XspI CTAG 4 cut(s) 590, 779, 836, 938
ZraI GACGTC 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.