pycom04g19740

Calcium-binding EF hand family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0014521
Physical Location & Seq
Forward (+)
21399595 .. 21401858
2264 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g19740.4

Sequence Viewer

Length: 975 bp
ATGTCGGACGGAGCTTTGACGGTCCTCGACGGCAGCCACCTGAGAGGCGTCGACCTCACCATGCTGGATGCTGACGCCAGCCTAACGGGAGCTCAGGTCCTCGACCTCGCCGTCTCCAAAGCCTCTTCCTCCCTCTTCGGCCTCTCGCTGCCTCAGAGCCTCAAGTCCTCTGCTTTGAGTCGCATCAGCCTCCAAGACGACGACGCTTTCCGAAGCGCGGCGCTCGATCGCGCGCAGGCTCTGAAGGTGATCGGCGAGTACATCGCGGCGATCGCCGATGAATTGAAAGATGATCCACTTGTCATATCTGTCTTGGACGGATACAATCTTCGACTGTTTTTAGAGGACGAGGATGATTTTGCAATGTTGGCGGAGAATCTGTTCACTGATCTGGATGTAGAAGATAAAGGAAAGATCAACAAGAATGAGATCGGGAATGCGCTTGCTCAAATGGGAGTTGAGATGGGTGTTCCTCCCGTTTCAGAATTTCCTCCTTTAAATGATATCTTGACAAAACATGAAGCTGATGGGGACGAAGAATTTGGCCAAGCACAATTTGCTCAGCTACTCCAGCCTGTCCTGCAGGAACTAGCCGAGGCCCTGGCCAAAAAGCATGTTGTTTCTATCCAGAGCATCAAGATCGTCAATGGCTCTAAGCTAAAAAAGCTTTTGGCTGATGAGAAGCAATTGAACAATGTCGTTGAGAAGATATTGCAAGAAGAGCACCATGGGAAGGACGGATTGGGGAATGCAGATAAAATTAGGAGTTTTCTTGAGAAAAATGGGCGAGAGTTGGGTCTGCCACCATACGAACCCGAGGCAGTTGCTCTTTTGTACGATGCAGTGTTTGCTGATCTAAACGCAGACAAGAGTGCTCCAGAAGTAGGCCAAGACAAATTTGGAACTCTTGTGAAGGAGATACTCGAGGAATTCGCAGATCAGCTAGAAGCGAGTCCTGTTTTTTGTGATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5 EF-hand_7

Protein Analysis

325

Amino Acids

35.04

Weight (kDa)

4.37

Isoelectric Point (pI)

35.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014521)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G28830
fragaria_vesca FvH4_6g02990
malus_domestica MD04G1224000.v1.1 MD12G1240800.v1.1
prunus_persica Prupe.6G342700_v2.0.a1
pyrus_communis pycom04g19740 pycom12g22050
rosa_chinensis RchiOBHm_Chr3g0451171
rosa_laevigata RLG00000025680
rosa_multiflora Rmu_co8512575.1_g000001
rosa_roxburghii Rroxscaffold_6G00428640
rosa_rugosa Rorug02G0632500
rosa_samantha Rh3AG035000 Rh3BG035400 Rh3CG034000 Rh3DG035000
rosa_wichuraiana Rw3G002570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 110
AccI GTMKAC 1 cut(s) 51
AccII CGCG 4 cut(s) 218, 231, 233, 266
AciI CCGC 3 cut(s) 218, 266, 371
AclWI GGATC 1 cut(s) 287
AcoI YGGCCR 2 cut(s) 544, 603
AcsI RAATTY 4 cut(s) 485, 539, 896, 929
AcuI CTGAAG 1 cut(s) 263
AcyI GRCGYC 2 cut(s) 48, 75
AfaI GTAC 2 cut(s) 260, 836
AfiI CCNNNNNNNGG 3 cut(s) 217, 733, 884
AgsI TTSAA 2 cut(s) 286, 691
AjnI CCWGG 1 cut(s) 600
AjuI GAANNNNNNNTTGG 2 cut(s) 725, 757
AloI GAACNNNNNNTCC 2 cut(s) 365, 397
AluBI AGCT 7 cut(s) 14, 92, 524, 565, 658, 667, 943
AluI AGCT 7 cut(s) 14, 92, 524, 565, 658, 667, 943
Alw21I GWGCWC 3 cut(s) 94, 726, 877
Alw26I GTCTC 1 cut(s) 118
AlwI GGATC 1 cut(s) 287
AlwNI CAGNNNCTG 1 cut(s) 241
Ama87I CYCGRG 2 cut(s) 815, 923
AoxI GGCC 5 cut(s) 139, 544, 597, 603, 886
ApeKI GCWGC 2 cut(s) 33, 148
ApoI RAATTY 4 cut(s) 485, 539, 896, 929
ArsI GACNNNNNNTTYG 2 cut(s) 524, 556
AsiSI GCGATCGC 1 cut(s) 273
Asp700I GAANNNNTTC 1 cut(s) 380
AspLEI GCGC 5 cut(s) 218, 223, 233, 235, 442
AspS9I GGNCC 3 cut(s) 22, 97, 598
AsuHPI GGTGA 2 cut(s) 49, 259
AvaI CYCGRG 2 cut(s) 815, 923
AvaII GGWCC 2 cut(s) 22, 97
BalI TGGCCA 2 cut(s) 546, 605
BanII GRGCYC 1 cut(s) 94
Bbv12I GWGCWC 3 cut(s) 94, 726, 877
BbvI GCAGC 2 cut(s) 45, 135
BccI CCATC 2 cut(s) 457, 521
BceAI ACGGC 2 cut(s) 46, 95
BcgI CGANNNNNNTGC 4 cut(s) 622, 656, 806, 840
BciT130I CCWGG 1 cut(s) 602
BciVI GTATCC 1 cut(s) 314
BcoDI GTCTC 1 cut(s) 118
BfaI CTAG 2 cut(s) 590, 944
BfmI CTRYAG 1 cut(s) 581
BfoI RGCGCY 1 cut(s) 224
BfuI GTATCC 1 cut(s) 314
BisI GCNGC 4 cut(s) 34, 149, 219, 267
BlpI GCTNAGC 1 cut(s) 561
BlsI GCNGC 4 cut(s) 35, 150, 220, 268
Bme1390I CCNGG 1 cut(s) 602
Bme18I GGWCC 2 cut(s) 22, 97
BmeT110I CYCGRG 2 cut(s) 815, 923
BmgT120I GGNCC 3 cut(s) 22, 97, 598
BmrFI CCNGG 1 cut(s) 602
BmsI GCATC 5 cut(s) 58, 192, 642, 829, 958
BpmI CTGGAG 2 cut(s) 554, 861
Bpu10I CCTNAGC 1 cut(s) 93
Bpu1102I GCTNAGC 1 cut(s) 561
BpuEI CTTGAG 2 cut(s) 146, 794
BsaHI GRCGYC 2 cut(s) 48, 75
BsaJI CCNNGG 4 cut(s) 594, 600, 727, 816
BsaXI ACNNNNNCTCC 4 cut(s) 81, 111, 365, 395
Bsc4I CCNNNNNNNGG 3 cut(s) 217, 733, 884
Bse3DI GCAATG 1 cut(s) 369
BseBI CCWGG 1 cut(s) 602
BseDI CCNNGG 4 cut(s) 594, 600, 727, 816
BseGI GGATG 3 cut(s) 73, 358, 400
BseLI CCNNNNNNNGG 3 cut(s) 217, 733, 884
BseMI GCAATG 1 cut(s) 369
BseMII CTCAG 4 cut(s) 32, 107, 167, 575
BsePI GCGCGC 1 cut(s) 231
BseXI GCAGC 2 cut(s) 45, 135
Bsh1236I CGCG 4 cut(s) 218, 231, 233, 266
Bsh1285I CGRYCG 2 cut(s) 229, 273
BshFI GGCC 5 cut(s) 141, 546, 599, 605, 888
BsiEI CGRYCG 2 cut(s) 229, 273
BsiHKAI GWGCWC 3 cut(s) 94, 726, 877
BsiHKCI CYCGRG 2 cut(s) 815, 923
BslFI GGGAC 1 cut(s) 545
BslI CCNNNNNNNGG 3 cut(s) 217, 733, 884
BsmAI GTCTC 1 cut(s) 118
BsmBI CGTCTC 1 cut(s) 118
BsmFI GGGAC 1 cut(s) 545
BsmI GAATGC 2 cut(s) 442, 754
BsnI GGCC 5 cut(s) 141, 546, 599, 605, 888
BsoBI CYCGRG 2 cut(s) 815, 923
Bsp1286I GDGCHC 3 cut(s) 94, 726, 877
Bsp1720I GCTNAGC 1 cut(s) 561
Bsp19I CCATGG 1 cut(s) 727
BspACI CCGC 3 cut(s) 218, 266, 371
BspANI GGCC 5 cut(s) 141, 546, 599, 605, 888
BspCNI CTCAG 4 cut(s) 33, 106, 166, 574
BspFNI CGCG 4 cut(s) 218, 231, 233, 266
BspMAI CTGCAG 1 cut(s) 585
BspPI GGATC 1 cut(s) 287
BspQI GCTCTTC 1 cut(s) 714
BsrDI GCAATG 1 cut(s) 369
BssECI CCNNGG 4 cut(s) 594, 600, 727, 816
BssHII GCGCGC 1 cut(s) 231
BssNI GRCGYC 2 cut(s) 48, 75
BssT1I CCWWGG 1 cut(s) 727
Bst2UI CCWGG 1 cut(s) 602
Bst4CI ACNGT 2 cut(s) 22, 336
Bst6I CTCTTC 3 cut(s) 130, 140, 714
BstACI GRCGYC 2 cut(s) 48, 75
BstAPI GCANNNNNTGC 2 cut(s) 557, 848
BstC8I GCNNGC 4 cut(s) 79, 233, 237, 444
BstDEI CTNAG 5 cut(s) 41, 93, 153, 561, 654
BstDSI CCRYGG 1 cut(s) 727
BstF5I GGATG 3 cut(s) 73, 358, 400
BstFNI CGCG 4 cut(s) 218, 231, 233, 266
BstH2I RGCGCY 1 cut(s) 224
BstHHI GCGC 5 cut(s) 218, 223, 233, 235, 442
BstMAI GTCTC 1 cut(s) 118
BstMCI CGRYCG 2 cut(s) 229, 273
BstMWI GCNNNNNNNGC 8 cut(s) 272, 368, 557, 571, 580, 664, 721, 848
BstNI CCWGG 1 cut(s) 602
BstNSI RCATGY 1 cut(s) 617
BstSCI CCNGG 1 cut(s) 600
BstSFI CTRYAG 1 cut(s) 581
BstUI CGCG 4 cut(s) 218, 231, 233, 266
BstV1I GCAGC 2 cut(s) 45, 135
BsuI GTATCC 1 cut(s) 314
BsuRI GGCC 5 cut(s) 141, 546, 599, 605, 888
BtgI CCRYGG 1 cut(s) 727
BtgZI GCGATG 1 cut(s) 247
BtsCI GGATG 3 cut(s) 73, 358, 400
BtsI GCAGTG 1 cut(s) 849
BtsIMutI CAGTG 2 cut(s) 384, 849
Cac8I GCNNGC 4 cut(s) 79, 233, 237, 444
CaiI CAGNNNCTG 1 cut(s) 241
CfoI GCGC 5 cut(s) 218, 223, 233, 235, 442
Cfr13I GGNCC 3 cut(s) 22, 97, 598
CseI GACGC 3 cut(s) 37, 83, 212
Csp6I GTAC 2 cut(s) 259, 835
CviAII CATG 4 cut(s) 61, 518, 614, 728
CviQI GTAC 2 cut(s) 259, 835
DdeI CTNAG 5 cut(s) 41, 93, 153, 561, 654
DraI TTTAAA 1 cut(s) 498
DrdI GACNNNNNNGTC 1 cut(s) 110
DseDI GACNNNNNNGTC 1 cut(s) 110
EaeI YGGCCR 2 cut(s) 544, 603
Eam1104I CTCTTC 3 cut(s) 130, 140, 714
EarI CTCTTC 3 cut(s) 130, 140, 714
EciI GGCGGA 1 cut(s) 386
Ecl136II GAGCTC 1 cut(s) 92
Eco130I CCWWGG 1 cut(s) 727
Eco24I GRGCYC 1 cut(s) 94
Eco32I GATATC 1 cut(s) 505
Eco47I GGWCC 2 cut(s) 22, 97
Eco53kI GAGCTC 1 cut(s) 92
Eco57I CTGAAG 1 cut(s) 263
Eco88I CYCGRG 2 cut(s) 815, 923
EcoICRI GAGCTC 1 cut(s) 92
EcoO109I RGGNCCY 2 cut(s) 97, 598
EcoRI GAATTC 1 cut(s) 929
EcoRII CCWGG 1 cut(s) 600
EcoRV GATATC 1 cut(s) 505
EcoT14I CCWWGG 1 cut(s) 727
EcoT38I GRGCYC 1 cut(s) 94
ErhI CCWWGG 1 cut(s) 727
Esp3I CGTCTC 1 cut(s) 118
FaeI CATG 4 cut(s) 64, 521, 617, 731
FaiI YATR 6 cut(s) 62, 305, 519, 615, 729, 808
FaqI GGGAC 1 cut(s) 545
FatI CATG 4 cut(s) 60, 517, 613, 727
FblI GTMKAC 1 cut(s) 51
Fnu4HI GCNGC 4 cut(s) 34, 149, 219, 267
FokI GGATG 3 cut(s) 80, 365, 407
FriOI GRGCYC 1 cut(s) 94
Fsp4HI GCNGC 4 cut(s) 34, 149, 219, 267
FspBI CTAG 2 cut(s) 590, 944
GlaI GCGC 5 cut(s) 217, 222, 232, 234, 441
GluI GCNGC 4 cut(s) 34, 149, 219, 267
GsuI CTGGAG 2 cut(s) 554, 861
HaeII RGCGCY 1 cut(s) 224
HaeIII GGCC 5 cut(s) 141, 546, 599, 605, 888
HgaI GACGC 3 cut(s) 37, 83, 212
HhaI GCGC 5 cut(s) 218, 223, 233, 235, 442
Hin1I GRCGYC 2 cut(s) 48, 75
Hin1II CATG 4 cut(s) 64, 521, 617, 731
Hin6I GCGC 5 cut(s) 216, 221, 231, 233, 440
HinP1I GCGC 5 cut(s) 216, 221, 231, 233, 440
HincII GTYRAC 1 cut(s) 52
HindII GTYRAC 1 cut(s) 52
HindIII AAGCTT 1 cut(s) 665
HinfI GANTC 3 cut(s) 178, 376, 952
HphI GGTGA 2 cut(s) 49, 259
Hpy166II GTNNAC 2 cut(s) 52, 384
Hpy188I TCNGA 5 cut(s) 7, 156, 212, 243, 484
Hpy188III TCNNGA 7 cut(s) 392, 433, 508, 628, 637, 773, 878
Hpy8I GTNNAC 2 cut(s) 52, 384
Hpy99I CGWCG 4 cut(s) 32, 53, 203, 206
HpyAV CCTTC 3 cut(s) 238, 727, 907
HpyCH4III ACNGT 2 cut(s) 22, 336
HpyCH4V TGCA 5 cut(s) 362, 583, 715, 752, 842
HpyF10VI GCNNNNNNNGC 8 cut(s) 272, 368, 557, 571, 580, 664, 721, 848
HpyF3I CTNAG 5 cut(s) 41, 93, 153, 561, 654
Hsp92I GRCGYC 2 cut(s) 48, 75
Hsp92II CATG 4 cut(s) 64, 521, 617, 731
HspAI GCGC 5 cut(s) 216, 221, 231, 233, 440
LguI GCTCTTC 1 cut(s) 714
LmnI GCTCC 3 cut(s) 11, 89, 880
Lsp1109I GCAGC 2 cut(s) 45, 135
LweI GCATC 5 cut(s) 58, 192, 642, 829, 958
MaeI CTAG 2 cut(s) 590, 944
MboII GAAGA 7 cut(s) 117, 127, 320, 413, 548, 718, 731
MfeI CAATTG 1 cut(s) 686
MhlI GDGCHC 3 cut(s) 94, 726, 877
MlsI TGGCCA 2 cut(s) 546, 605
MluCI AATT 8 cut(s) 281, 485, 539, 554, 686, 759, 896, 929
MluNI TGGCCA 2 cut(s) 546, 605
MlyI GAGTC 2 cut(s) 187, 961
Mox20I TGGCCA 2 cut(s) 546, 605
MroXI GAANNNNTTC 1 cut(s) 380
MscI TGGCCA 2 cut(s) 546, 605
MseI TTAA 1 cut(s) 497
Msp20I TGGCCA 2 cut(s) 546, 605
MspR9I CCNGG 1 cut(s) 602
MteI GCGCNGCGC 1 cut(s) 219
MunI CAATTG 1 cut(s) 686
Mva1269I GAATGC 2 cut(s) 442, 754
MvaI CCWGG 1 cut(s) 602
MvnI CGCG 4 cut(s) 218, 231, 233, 266
MwoI GCNNNNNNNGC 8 cut(s) 272, 368, 557, 571, 580, 664, 721, 848
NcoI CCATGG 1 cut(s) 727
NlaIII CATG 4 cut(s) 64, 521, 617, 731
NmeAIII GCCGAG 1 cut(s) 619
NspI RCATGY 1 cut(s) 617
PaeR7I CTCGAG 1 cut(s) 923
PauI GCGCGC 1 cut(s) 231
PciSI GCTCTTC 1 cut(s) 714
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PctI GAATGC 2 cut(s) 442, 754
PdmI GAANNNNTTC 1 cut(s) 380
PfeI GAWTC 1 cut(s) 376
PkrI GCNGC 4 cut(s) 35, 150, 220, 268
Ple19I CGATCG 2 cut(s) 229, 273
PleI GAGTC 2 cut(s) 186, 960
PpsI GAGTC 2 cut(s) 186, 960
PpuMI RGGWCCY 1 cut(s) 97
Psp124BI GAGCTC 1 cut(s) 94
Psp5II RGGWCCY 1 cut(s) 97
Psp6I CCWGG 1 cut(s) 600
PspGI CCWGG 1 cut(s) 600
PspPI GGNCC 3 cut(s) 22, 97, 598
PspPPI RGGWCCY 1 cut(s) 97
PspXI VCTCGAGB 1 cut(s) 923
PstI CTGCAG 1 cut(s) 585
PstNI CAGNNNCTG 1 cut(s) 241
PteI GCGCGC 1 cut(s) 231
PvuI CGATCG 2 cut(s) 229, 273
RgaI GCGATCGC 1 cut(s) 273
RsaI GTAC 2 cut(s) 260, 836
RsaNI GTAC 2 cut(s) 259, 835
SacI GAGCTC 1 cut(s) 94
SalI GTCGAC 1 cut(s) 50
SapI GCTCTTC 1 cut(s) 714
SaqAI TTAA 1 cut(s) 497
SatI GCNGC 4 cut(s) 34, 149, 219, 267
Sau96I GGNCC 3 cut(s) 22, 97, 598
SbfI CCTGCAGG 1 cut(s) 585
SchI GAGTC 2 cut(s) 187, 961
ScrFI CCNGG 1 cut(s) 602
SdaI CCTGCAGG 1 cut(s) 585
SduI GDGCHC 3 cut(s) 94, 726, 877
SfaAI GCGATCGC 1 cut(s) 273
SfaNI GCATC 5 cut(s) 58, 192, 642, 829, 958
SfcI CTRYAG 1 cut(s) 581
Sfr274I CTCGAG 1 cut(s) 923
SgfI GCGATCGC 1 cut(s) 273
SinI GGWCC 2 cut(s) 22, 97
SlaI CTCGAG 1 cut(s) 923
SmlI CTYRAG 3 cut(s) 161, 773, 923
SmoI CTYRAG 3 cut(s) 161, 773, 923
Sse8387I CCTGCAGG 1 cut(s) 585
Sse9I AATT 8 cut(s) 281, 485, 539, 554, 686, 759, 896, 929
SsiI CCGC 3 cut(s) 218, 266, 371
SspMI CTAG 2 cut(s) 590, 944
SstI GAGCTC 1 cut(s) 94
StyD4I CCNGG 1 cut(s) 600
StyI CCWWGG 1 cut(s) 727
TaaI ACNGT 2 cut(s) 22, 336
TaqI TCGA 6 cut(s) 27, 51, 102, 225, 331, 924
TasI AATT 8 cut(s) 281, 485, 539, 554, 686, 759, 896, 929
TatI WGTACW 1 cut(s) 258
TauI GCSGC 2 cut(s) 221, 269
TfiI GAWTC 1 cut(s) 376
Tru1I TTAA 1 cut(s) 497
Tru9I TTAA 1 cut(s) 497
TscAI CASTG 2 cut(s) 391, 849
TseI GCWGC 2 cut(s) 33, 148
TspDTI ATGAA 2 cut(s) 294, 534
TspGWI ACGGA 3 cut(s) 24, 333, 753
TspRI CASTG 2 cut(s) 391, 849
VpaK11BI GGWCC 2 cut(s) 22, 97
XapI RAATTY 4 cut(s) 485, 539, 896, 929
XceI RCATGY 1 cut(s) 617
XcmI CCANNNNNNNNNTGG 1 cut(s) 896
XhoI CTCGAG 1 cut(s) 923
XmiI GTMKAC 1 cut(s) 51
XmnI GAANNNNTTC 1 cut(s) 380
XspI CTAG 2 cut(s) 590, 944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.