Rh3AG035000

Calcium-binding EF hand family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0014521
Physical Location & Seq
Reverse (-)
2332419 .. 2337093
4675 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG035000.1

Sequence Viewer

Length: 555 bp
ATGTCCGACGGAGCTTTAACGGTCCTCGACGGCACTCACCTGAGAGCCGTCGACCTCTCTCTTCCCGACTCTGACGTCATCCTGACCGGAGGTCACGTGCTCGACCTCGCCGACTCCAAAGCCTCTTCGTCTCTTTTCGGCCTGTCCTTGCCTCAGGGACTCAAGTCCTCTGCTTTGAAACGCGTCAATGTCCAAGACGACGACGTTTTCCGTCAAGCCGAGCTCGATCGCCACCAGGCTCTGAAGCTCGCCGGCGACTACATCGCCGCGATCGCCGATGAGTTGAAAGATGATCCGCTTGTCATATCTATCTTGGATGGAAACACGATTCGGCTGTTTTTGGAGGATGAGGATGACTTTGCAATGCTAGCGGAGAATCTGTTCACTGATCTTGATGCAGAAGATGTAGGAAAGATCAACAAGAGCGCGATCCGGAGTGCGCTGGTTCACATGGGAGTTGAGATGGGTGTCCCTCCCGCTTCAGGTTATTTTTGGATTCATTGCTATGGCTTTAATTCTAGGATTGTTGGAAAGTATGTTCTTTCTTTTTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5 EF-hand_7

Protein Analysis

184

Amino Acids

19.94

Weight (kDa)

4.5

Isoelectric Point (pI)

29.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014521)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G28830
fragaria_vesca FvH4_6g02990
malus_domestica MD04G1224000.v1.1 MD12G1240800.v1.1
prunus_persica Prupe.6G342700_v2.0.a1
pyrus_communis pycom04g19740 pycom12g22050
rosa_chinensis RchiOBHm_Chr3g0451171
rosa_laevigata RLG00000025680
rosa_multiflora Rmu_co8512575.1_g000001
rosa_roxburghii Rroxscaffold_6G00428640
rosa_rugosa Rorug02G0632500
rosa_samantha Rh3AG035000 Rh3BG035400 Rh3CG034000 Rh3DG035000
rosa_wichuraiana Rw3G002570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 74
AatII GACGTC 1 cut(s) 78
AccI GTMKAC 1 cut(s) 51
AccII CGCG 3 cut(s) 183, 269, 428
AccIII TCCGGA 1 cut(s) 432
AciI CCGC 4 cut(s) 267, 296, 371, 477
AclWI GGATC 2 cut(s) 287, 424
AcuI CTGAAG 2 cut(s) 263, 465
AcvI CACGTG 1 cut(s) 97
AcyI GRCGYC 1 cut(s) 75
AfiI CCNNNNNNNGG 1 cut(s) 482
AflIII ACRYGT 1 cut(s) 181
AgsI TTSAA 2 cut(s) 178, 286
AhdI GACNNNNNGTC 1 cut(s) 90
AjnI CCWGG 1 cut(s) 234
AloI GAACNNNNNNTCC 4 cut(s) 365, 397, 522, 554
AluBI AGCT 3 cut(s) 14, 223, 247
AluI AGCT 3 cut(s) 14, 223, 247
Alw21I GWGCWC 2 cut(s) 102, 225
Alw26I GTCTC 1 cut(s) 135
AlwI GGATC 2 cut(s) 287, 424
AlwNI CAGNNNCTG 1 cut(s) 241
Aor13HI TCCGGA 1 cut(s) 432
AoxI GGCC 1 cut(s) 139
AsiSI GCGATCGC 1 cut(s) 273
Asp700I GAANNNNTTC 1 cut(s) 380
AspLEI GCGC 2 cut(s) 428, 442
AspS9I GGNCC 1 cut(s) 22
AsuHPI GGTGA 1 cut(s) 29
AsuNHI GCTAGC 1 cut(s) 367
AvaII GGWCC 1 cut(s) 22
AxyI CCTNAGG 1 cut(s) 153
BanII GRGCYC 1 cut(s) 225
BbrPI CACGTG 1 cut(s) 97
Bbv12I GWGCWC 2 cut(s) 102, 225
BccI CCATC 2 cut(s) 311, 457
BceAI ACGGC 2 cut(s) 32, 46
BciT130I CCWGG 1 cut(s) 236
BcoDI GTCTC 1 cut(s) 135
BfaI CTAG 2 cut(s) 368, 519
BisI GCNGC 1 cut(s) 267
BlsI GCNGC 1 cut(s) 268
Bme1390I CCNGG 1 cut(s) 236
Bme18I GGWCC 1 cut(s) 22
BmeRI GACNNNNNGTC 1 cut(s) 90
BmgT120I GGNCC 1 cut(s) 22
BmrFI CCNGG 1 cut(s) 236
BmsI GCATC 1 cut(s) 385
BmtI GCTAGC 1 cut(s) 371
BoxI GACNNNNGTC 1 cut(s) 163
BpuEI CTTGAG 1 cut(s) 146
BsaAI YACGTR 1 cut(s) 97
BsaHI GRCGYC 1 cut(s) 75
BsaWI WCCGGW 2 cut(s) 86, 432
BsaXI ACNNNNNCTCC 4 cut(s) 81, 111, 365, 395
Bsc4I CCNNNNNNNGG 1 cut(s) 482
Bse118I RCCGGY 1 cut(s) 251
Bse21I CCTNAGG 1 cut(s) 153
Bse3DI GCAATG 2 cut(s) 369, 499
BseAI TCCGGA 1 cut(s) 432
BseBI CCWGG 1 cut(s) 236
BseGI GGATG 4 cut(s) 78, 322, 352, 358
BseLI CCNNNNNNNGG 1 cut(s) 482
BseMI GCAATG 2 cut(s) 369, 499
BseMII CTCAG 2 cut(s) 32, 167
Bsh1236I CGCG 3 cut(s) 183, 269, 428
Bsh1285I CGRYCG 2 cut(s) 229, 273
BshFI GGCC 1 cut(s) 141
BsiEI CGRYCG 2 cut(s) 229, 273
BsiHKAI GWGCWC 2 cut(s) 102, 225
BsiSI CCGG 3 cut(s) 87, 252, 433
BslFI GGGAC 2 cut(s) 171, 455
BslI CCNNNNNNNGG 1 cut(s) 482
BsmAI GTCTC 1 cut(s) 135
BsmBI CGTCTC 1 cut(s) 135
BsmFI GGGAC 2 cut(s) 171, 455
BsnI GGCC 1 cut(s) 141
Bsp1286I GDGCHC 2 cut(s) 102, 225
Bsp13I TCCGGA 1 cut(s) 432
Bsp143I GATC 6 cut(s) 226, 270, 292, 388, 414, 429
BspACI CCGC 4 cut(s) 267, 296, 371, 477
BspANI GGCC 1 cut(s) 141
BspCNI CTCAG 2 cut(s) 33, 166
BspEI TCCGGA 1 cut(s) 432
BspFNI CGCG 3 cut(s) 183, 269, 428
BspOI GCTAGC 1 cut(s) 371
BspPI GGATC 2 cut(s) 287, 424
BsrDI GCAATG 2 cut(s) 369, 499
BsrFI RCCGGY 1 cut(s) 251
BssAI RCCGGY 1 cut(s) 251
BssMI GATC 6 cut(s) 226, 270, 292, 388, 414, 429
BssNI GRCGYC 1 cut(s) 75
Bst2UI CCWGG 1 cut(s) 236
Bst4CI ACNGT 1 cut(s) 22
Bst6I CTCTTC 2 cut(s) 66, 130
BstACI GRCGYC 1 cut(s) 75
BstBAI YACGTR 1 cut(s) 97
BstC8I GCNNGC 3 cut(s) 249, 253, 369
BstDEI CTNAG 2 cut(s) 41, 153
BstF5I GGATG 4 cut(s) 78, 322, 352, 358
BstFNI CGCG 3 cut(s) 183, 269, 428
BstHHI GCGC 2 cut(s) 428, 442
BstKTI GATC 6 cut(s) 229, 273, 295, 391, 417, 432
BstMAI GTCTC 1 cut(s) 135
BstMBI GATC 6 cut(s) 226, 270, 292, 388, 414, 429
BstMCI CGRYCG 2 cut(s) 229, 273
BstMWI GCNNNNNNNGC 2 cut(s) 272, 368
BstNI CCWGG 1 cut(s) 236
BstPAI GACNNNNGTC 1 cut(s) 163
BstSCI CCNGG 1 cut(s) 234
BstUI CGCG 3 cut(s) 183, 269, 428
Bsu36I CCTNAGG 1 cut(s) 153
BsuRI GGCC 1 cut(s) 141
BtgZI GCGATG 1 cut(s) 247
BtsCI GGATG 4 cut(s) 78, 322, 352, 358
BtsIMutI CAGTG 1 cut(s) 384
Cac8I GCNNGC 3 cut(s) 249, 253, 369
CaiI CAGNNNCTG 1 cut(s) 241
CfoI GCGC 2 cut(s) 428, 442
Cfr10I RCCGGY 1 cut(s) 251
Cfr13I GGNCC 1 cut(s) 22
CseI GACGC 1 cut(s) 172
CviAII CATG 1 cut(s) 451
DdeI CTNAG 2 cut(s) 41, 153
DpnI GATC 6 cut(s) 228, 272, 294, 390, 416, 431
DpnII GATC 6 cut(s) 226, 270, 292, 388, 414, 429
DrdI GACNNNNNNGTC 1 cut(s) 74
DriI GACNNNNNGTC 1 cut(s) 90
DseDI GACNNNNNNGTC 1 cut(s) 74
Eam1104I CTCTTC 2 cut(s) 66, 130
Eam1105I GACNNNNNGTC 1 cut(s) 90
EarI CTCTTC 2 cut(s) 66, 130
Ecl136II GAGCTC 1 cut(s) 223
Eco24I GRGCYC 1 cut(s) 225
Eco47I GGWCC 1 cut(s) 22
Eco53kI GAGCTC 1 cut(s) 223
Eco57I CTGAAG 2 cut(s) 263, 465
Eco72I CACGTG 1 cut(s) 97
Eco81I CCTNAGG 1 cut(s) 153
EcoICRI GAGCTC 1 cut(s) 223
EcoRII CCWGG 1 cut(s) 234
EcoT38I GRGCYC 1 cut(s) 225
Esp3I CGTCTC 1 cut(s) 135
FaeI CATG 1 cut(s) 454
FaiI YATR 4 cut(s) 305, 452, 507, 537
FaqI GGGAC 2 cut(s) 171, 455
FatI CATG 1 cut(s) 450
FauI CCCGC 1 cut(s) 484
FblI GTMKAC 1 cut(s) 51
Fnu4HI GCNGC 1 cut(s) 267
FokI GGATG 4 cut(s) 65, 329, 359, 365
FriOI GRGCYC 1 cut(s) 225
Fsp4HI GCNGC 1 cut(s) 267
FspBI CTAG 2 cut(s) 368, 519
GlaI GCGC 2 cut(s) 427, 441
GluI GCNGC 1 cut(s) 267
HaeIII GGCC 1 cut(s) 141
HapII CCGG 3 cut(s) 87, 252, 433
HgaI GACGC 1 cut(s) 172
HhaI GCGC 2 cut(s) 428, 442
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 1 cut(s) 454
Hin6I GCGC 2 cut(s) 426, 440
HinP1I GCGC 2 cut(s) 426, 440
HincII GTYRAC 1 cut(s) 52
HindII GTYRAC 1 cut(s) 52
HinfI GANTC 6 cut(s) 68, 113, 159, 328, 376, 496
HpaII CCGG 3 cut(s) 87, 252, 433
HphI GGTGA 1 cut(s) 29
Hpy166II GTNNAC 3 cut(s) 52, 384, 448
Hpy188I TCNGA 3 cut(s) 7, 73, 243
Hpy188III TCNNGA 4 cut(s) 65, 82, 392, 433
Hpy8I GTNNAC 3 cut(s) 52, 384, 448
Hpy99I CGWCG 5 cut(s) 11, 32, 53, 203, 206
HpyCH4III ACNGT 1 cut(s) 22
HpyCH4IV ACGT 3 cut(s) 75, 96, 204
HpyCH4V TGCA 2 cut(s) 362, 398
HpyF10VI GCNNNNNNNGC 2 cut(s) 272, 368
HpyF3I CTNAG 2 cut(s) 41, 153
HpySE526I ACGT 3 cut(s) 75, 96, 204
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 1 cut(s) 454
HspAI GCGC 2 cut(s) 426, 440
Kpn2I TCCGGA 1 cut(s) 432
KroI GCCGGC 1 cut(s) 251
KroNI GCCGGC 1 cut(s) 253
Kzo9I GATC 6 cut(s) 226, 270, 292, 388, 414, 429
LmnI GCTCC 1 cut(s) 11
LweI GCATC 1 cut(s) 385
MaeI CTAG 2 cut(s) 368, 519
MaeII ACGT 3 cut(s) 75, 96, 204
MaeIII GTNAC 1 cut(s) 92
MalI GATC 6 cut(s) 228, 272, 294, 390, 416, 431
MboI GATC 6 cut(s) 226, 270, 292, 388, 414, 429
MboII GAAGA 3 cut(s) 53, 117, 413
MhlI GDGCHC 2 cut(s) 102, 225
MluCI AATT 1 cut(s) 514
MluI ACGCGT 1 cut(s) 181
MlyI GAGTC 3 cut(s) 62, 107, 153
MmeI TCCRAC 2 cut(s) 30, 508
MreI CGCCGGCG 1 cut(s) 251
MroI TCCGGA 1 cut(s) 432
MroNI GCCGGC 1 cut(s) 251
MroXI GAANNNNTTC 1 cut(s) 380
MseI TTAA 2 cut(s) 17, 513
MslI CAYNNNNRTG 1 cut(s) 504
MspI CCGG 3 cut(s) 87, 252, 433
MspR9I CCNGG 1 cut(s) 236
MvaI CCWGG 1 cut(s) 236
MvnI CGCG 3 cut(s) 183, 269, 428
MwoI GCNNNNNNNGC 2 cut(s) 272, 368
NaeI GCCGGC 1 cut(s) 253
NdeII GATC 6 cut(s) 226, 270, 292, 388, 414, 429
NgoMIV GCCGGC 1 cut(s) 251
NheI GCTAGC 1 cut(s) 367
NlaIII CATG 1 cut(s) 454
NmeAIII GCCGAG 1 cut(s) 244
NmuCI GTSAC 1 cut(s) 92
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PdiI GCCGGC 1 cut(s) 253
PdmI GAANNNNTTC 1 cut(s) 380
PfeI GAWTC 3 cut(s) 328, 376, 496
PkrI GCNGC 1 cut(s) 268
Ple19I CGATCG 2 cut(s) 229, 273
PleI GAGTC 3 cut(s) 62, 107, 153
PmaCI CACGTG 1 cut(s) 97
PmlI CACGTG 1 cut(s) 97
PpsI GAGTC 3 cut(s) 62, 107, 153
Ppu21I YACGTR 1 cut(s) 97
PshAI GACNNNNGTC 1 cut(s) 163
Psp124BI GAGCTC 1 cut(s) 225
Psp6I CCWGG 1 cut(s) 234
PspCI CACGTG 1 cut(s) 97
PspGI CCWGG 1 cut(s) 234
PspPI GGNCC 1 cut(s) 22
PstNI CAGNNNCTG 1 cut(s) 241
PvuI CGATCG 2 cut(s) 229, 273
RgaI GCGATCGC 1 cut(s) 273
RseI CAYNNNNRTG 1 cut(s) 504
SacI GAGCTC 1 cut(s) 225
SalI GTCGAC 1 cut(s) 50
SaqAI TTAA 2 cut(s) 17, 513
SatI GCNGC 1 cut(s) 267
Sau3AI GATC 6 cut(s) 226, 270, 292, 388, 414, 429
Sau96I GGNCC 1 cut(s) 22
SchI GAGTC 3 cut(s) 62, 107, 153
ScrFI CCNGG 1 cut(s) 236
SduI GDGCHC 2 cut(s) 102, 225
SfaAI GCGATCGC 1 cut(s) 273
SfaNI GCATC 1 cut(s) 385
SgfI GCGATCGC 1 cut(s) 273
SgrAI CRCCGGYG 1 cut(s) 251
SinI GGWCC 1 cut(s) 22
SmiMI CAYNNNNRTG 1 cut(s) 504
SmlI CTYRAG 1 cut(s) 161
SmoI CTYRAG 1 cut(s) 161
Sse9I AATT 1 cut(s) 514
SsiI CCGC 4 cut(s) 267, 296, 371, 477
SspMI CTAG 2 cut(s) 368, 519
SstI GAGCTC 1 cut(s) 225
StyD4I CCNGG 1 cut(s) 234
TaaI ACNGT 1 cut(s) 22
TaiI ACGT 3 cut(s) 78, 99, 207
TaqI TCGA 4 cut(s) 27, 51, 102, 225
TasI AATT 1 cut(s) 514
TauI GCSGC 1 cut(s) 269
TfiI GAWTC 3 cut(s) 328, 376, 496
Tru1I TTAA 2 cut(s) 17, 513
Tru9I TTAA 2 cut(s) 17, 513
TscAI CASTG 1 cut(s) 391
TseFI GTSAC 1 cut(s) 92
Tsp45I GTSAC 1 cut(s) 92
TspDTI ATGAA 1 cut(s) 488
TspGWI ACGGA 2 cut(s) 24, 200
TspRI CASTG 1 cut(s) 391
VpaK11BI GGWCC 1 cut(s) 22
XmiI GTMKAC 1 cut(s) 51
XmnI GAANNNNTTC 1 cut(s) 380
XspI CTAG 2 cut(s) 368, 519
ZraI GACGTC 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.