MD12G1240800.v1.1

Calcium-binding EF hand family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0014521
Physical Location & Seq
Forward (+)
31289025 .. 31292530
3506 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1240800.v1.1.491

Sequence Viewer

Length: 966 bp
ATGTCGGACGCGACTTTAACGGTCCTCGACGGGAGCCACCTGAGAGCCGTCGACCTTACTCTGCCGGATGCTGACGCCAGCTTAACGGGAGCTCAGGTCCTCGACCTCGCCGTCTCCAAAGCCTCTTCCTCCCTCTTCGGCCTCTCGCTGCCTCAGAGCCTCAAGTCCTCTGCTTTGAGACGCATCGGCCTCCAAGACGACGACGTTTTCCGAAGCGCGGAGCTCGATCGCGCCCAGGCTCTGAAGGTGATCGGCGAGTACATCACGGCGATCGCCGATGAATTGAAAGATGATCCACTTGTCATATCTGTCTTGGACGGGTACACTATTCGACTGTTTTTAGAGGACGAGGATGATTTTGCAATGTTAGCGGAGAATCTGTTCACTGATCTGGATGTAGAAGATAAAGGAAAGATCAACAAGAATGAGATCCGGAATGCGCTTGTTCACATGGGAGTTGAGATGGGCGTTCCTCCCGTTTCAGAATTTCCTCCCCTAAGTGATATCTTGACAAAACACGAAGCTGATGGGGATGAAGAACTGGGCCAAGCACAATTTGCTCAGCTACTCCAGCCTGTCCTGCAGGAACTAGCCGAGGCCCTGGCTAAAAAGCATGTTGTTTTTATCCAGAGCATCAAGATTGTCAATGGTTCTAAGCTAAGAAAGCTTTTGGCTGATGAGAAGCAATTGAACGATGTCGTTGAGAAGATATTGCAAGAAGATCACCACGGAAAGGACGGATTGGGGAATGCAGAAAAAATTAGGAGTTTTCTTGTGAAAAATGGGCAAGAGTTGGGTCTGCCACCATACGAGGCCGAGGCAGTTGATCTTTTGTATGATGCAGTGTTTGCTGATCTAGACGTAGACAAGAGTGCTCCGGAAGACAAATGTGGAAATCTTGTGAAGGAGATACTCAAGGAATTCGCAGATCAACTGGAAGTGAGTCCTGTTTTTTACAATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5 EF-hand_7

Protein Analysis

322

Amino Acids

35.13

Weight (kDa)

4.44

Isoelectric Point (pI)

37.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014521)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G28830
fragaria_vesca FvH4_6g02990
malus_domestica MD04G1224000.v1.1 MD12G1240800.v1.1
prunus_persica Prupe.6G342700_v2.0.a1
pyrus_communis pycom04g19740 pycom12g22050
rosa_chinensis RchiOBHm_Chr3g0451171
rosa_laevigata RLG00000025680
rosa_multiflora Rmu_co8512575.1_g000001
rosa_roxburghii Rroxscaffold_6G00428640
rosa_rugosa Rorug02G0632500
rosa_samantha Rh3AG035000 Rh3BG035400 Rh3CG034000 Rh3DG035000
rosa_wichuraiana Rw3G002570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 110
AccI GTMKAC 2 cut(s) 51, 864
AccII CGCG 3 cut(s) 11, 218, 231
AccIII TCCGGA 2 cut(s) 432, 877
AciI CCGC 2 cut(s) 218, 371
AclWI GGATC 2 cut(s) 287, 424
AcsI RAATTY 2 cut(s) 485, 920
AcuI CTGAAG 1 cut(s) 263
AcyI GRCGYC 1 cut(s) 75
AfaI GTAC 2 cut(s) 260, 323
AfiI CCNNNNNNNGG 2 cut(s) 217, 733
AgsI TTSAA 2 cut(s) 286, 691
AjnI CCWGG 2 cut(s) 234, 600
AloI GAACNNNNNNTCC 2 cut(s) 365, 397
AluBI AGCT 7 cut(s) 81, 92, 223, 524, 565, 658, 667
AluI AGCT 7 cut(s) 81, 92, 223, 524, 565, 658, 667
Alw21I GWGCWC 3 cut(s) 94, 225, 877
Alw26I GTCTC 2 cut(s) 118, 172
AlwI GGATC 2 cut(s) 287, 424
AlwNI CAGNNNCTG 1 cut(s) 241
Aor13HI TCCGGA 2 cut(s) 432, 877
AoxI GGCC 5 cut(s) 139, 187, 544, 597, 813
ApeKI GCWGC 1 cut(s) 148
ApoI RAATTY 2 cut(s) 485, 920
AsiSI GCGATCGC 1 cut(s) 273
Asp700I GAANNNNTTC 1 cut(s) 380
AspLEI GCGC 3 cut(s) 218, 233, 442
AspS9I GGNCC 4 cut(s) 22, 97, 544, 598
AsuHPI GGTGA 2 cut(s) 259, 716
AvaII GGWCC 2 cut(s) 22, 97
BanII GRGCYC 2 cut(s) 94, 225
BbsI GAAGAC 1 cut(s) 888
Bbv12I GWGCWC 3 cut(s) 94, 225, 877
BbvI GCAGC 1 cut(s) 135
BccI CCATC 2 cut(s) 457, 521
BceAI ACGGC 3 cut(s) 32, 95, 282
BciT130I CCWGG 2 cut(s) 236, 602
BcoDI GTCTC 2 cut(s) 118, 172
BfaI CTAG 2 cut(s) 590, 857
BfmI CTRYAG 1 cut(s) 581
BisI GCNGC 1 cut(s) 149
BlpI GCTNAGC 1 cut(s) 561
BlsI GCNGC 1 cut(s) 150
Bme1390I CCNGG 2 cut(s) 236, 602
Bme18I GGWCC 2 cut(s) 22, 97
BmgT120I GGNCC 4 cut(s) 22, 97, 544, 598
BmiI GGNNCC 1 cut(s) 35
BmrFI CCNGG 2 cut(s) 236, 602
BmrI ACTGGG 1 cut(s) 551
BmsI GCATC 4 cut(s) 58, 192, 642, 829
BmuI ACTGGG 1 cut(s) 551
BpiI GAAGAC 1 cut(s) 888
BpmI CTGGAG 1 cut(s) 554
Bpu10I CCTNAGC 1 cut(s) 93
Bpu1102I GCTNAGC 1 cut(s) 561
BpuEI CTTGAG 2 cut(s) 146, 899
BsaHI GRCGYC 1 cut(s) 75
BsaJI CCNNGG 5 cut(s) 234, 594, 600, 727, 816
BsaWI WCCGGW 2 cut(s) 432, 877
BsaXI ACNNNNNCTCC 4 cut(s) 81, 111, 365, 395
Bsc4I CCNNNNNNNGG 2 cut(s) 217, 733
Bse1I ACTGG 2 cut(s) 546, 939
Bse3DI GCAATG 1 cut(s) 369
BseAI TCCGGA 2 cut(s) 432, 877
BseBI CCWGG 2 cut(s) 236, 602
BseDI CCNNGG 5 cut(s) 234, 594, 600, 727, 816
BseGI GGATG 4 cut(s) 73, 358, 400, 538
BseLI CCNNNNNNNGG 2 cut(s) 217, 733
BseMI GCAATG 1 cut(s) 369
BseMII CTCAG 4 cut(s) 32, 107, 167, 575
BseNI ACTGG 2 cut(s) 546, 939
BseXI GCAGC 1 cut(s) 135
Bsh1236I CGCG 3 cut(s) 11, 218, 231
Bsh1285I CGRYCG 2 cut(s) 229, 273
BshFI GGCC 5 cut(s) 141, 189, 546, 599, 815
BsiEI CGRYCG 2 cut(s) 229, 273
BsiHKAI GWGCWC 3 cut(s) 94, 225, 877
BsiSI CCGG 3 cut(s) 65, 433, 878
BslI CCNNNNNNNGG 2 cut(s) 217, 733
BsmAI GTCTC 2 cut(s) 118, 172
BsmBI CGTCTC 2 cut(s) 118, 172
BsmI GAATGC 2 cut(s) 442, 754
BsnI GGCC 5 cut(s) 141, 189, 546, 599, 815
Bsp1286I GDGCHC 3 cut(s) 94, 225, 877
Bsp13I TCCGGA 2 cut(s) 432, 877
Bsp1720I GCTNAGC 1 cut(s) 561
BspACI CCGC 2 cut(s) 218, 371
BspANI GGCC 5 cut(s) 141, 189, 546, 599, 815
BspCNI CTCAG 4 cut(s) 33, 106, 166, 574
BspEI TCCGGA 2 cut(s) 432, 877
BspFNI CGCG 3 cut(s) 11, 218, 231
BspLI GGNNCC 1 cut(s) 35
BspMAI CTGCAG 1 cut(s) 585
BspPI GGATC 2 cut(s) 287, 424
BsrDI GCAATG 1 cut(s) 369
BsrI ACTGG 2 cut(s) 546, 939
BssECI CCNNGG 5 cut(s) 234, 594, 600, 727, 816
BssNI GRCGYC 1 cut(s) 75
Bst2UI CCWGG 2 cut(s) 236, 602
Bst4CI ACNGT 2 cut(s) 22, 336
Bst6I CTCTTC 2 cut(s) 130, 140
BstACI GRCGYC 1 cut(s) 75
BstAPI GCANNNNNTGC 2 cut(s) 557, 848
BstC8I GCNNGC 1 cut(s) 79
BstDEI CTNAG 7 cut(s) 41, 93, 153, 497, 561, 654, 659
BstDSI CCRYGG 1 cut(s) 727
BstF5I GGATG 4 cut(s) 73, 358, 400, 538
BstFNI CGCG 3 cut(s) 11, 218, 231
BstHHI GCGC 3 cut(s) 218, 233, 442
BstMAI GTCTC 2 cut(s) 118, 172
BstMCI CGRYCG 2 cut(s) 229, 273
BstMWI GCNNNNNNNGC 6 cut(s) 368, 557, 571, 580, 664, 848
BstNI CCWGG 2 cut(s) 236, 602
BstNSI RCATGY 1 cut(s) 617
BstSCI CCNGG 2 cut(s) 234, 600
BstSFI CTRYAG 1 cut(s) 581
BstUI CGCG 3 cut(s) 11, 218, 231
BstV1I GCAGC 1 cut(s) 135
BstV2I GAAGAC 1 cut(s) 888
BstX2I RGATCY 1 cut(s) 429
BstYI RGATCY 1 cut(s) 429
BsuRI GGCC 5 cut(s) 141, 189, 546, 599, 815
BtgI CCRYGG 1 cut(s) 727
BtsCI GGATG 4 cut(s) 73, 358, 400, 538
BtsI GCAGTG 1 cut(s) 849
BtsIMutI CAGTG 2 cut(s) 384, 849
Cac8I GCNNGC 1 cut(s) 79
CaiI CAGNNNCTG 1 cut(s) 241
CfoI GCGC 3 cut(s) 218, 233, 442
Cfr13I GGNCC 4 cut(s) 22, 97, 544, 598
CseI GACGC 3 cut(s) 17, 83, 189
Csp6I GTAC 2 cut(s) 259, 322
CviAII CATG 2 cut(s) 451, 614
CviQI GTAC 2 cut(s) 259, 322
DdeI CTNAG 7 cut(s) 41, 93, 153, 497, 561, 654, 659
DrdI GACNNNNNNGTC 1 cut(s) 110
DseDI GACNNNNNNGTC 1 cut(s) 110
Eam1104I CTCTTC 2 cut(s) 130, 140
EarI CTCTTC 2 cut(s) 130, 140
Ecl136II GAGCTC 2 cut(s) 92, 223
Eco24I GRGCYC 2 cut(s) 94, 225
Eco32I GATATC 1 cut(s) 505
Eco47I GGWCC 2 cut(s) 22, 97
Eco53kI GAGCTC 2 cut(s) 92, 223
Eco57I CTGAAG 1 cut(s) 263
EcoICRI GAGCTC 2 cut(s) 92, 223
EcoO109I RGGNCCY 2 cut(s) 97, 598
EcoRI GAATTC 1 cut(s) 920
EcoRII CCWGG 2 cut(s) 234, 600
EcoRV GATATC 1 cut(s) 505
EcoT38I GRGCYC 2 cut(s) 94, 225
Esp3I CGTCTC 2 cut(s) 118, 172
FaeI CATG 2 cut(s) 454, 617
FaiI YATR 5 cut(s) 305, 452, 615, 808, 837
FatI CATG 2 cut(s) 450, 613
FblI GTMKAC 2 cut(s) 51, 864
Fnu4HI GCNGC 1 cut(s) 149
FokI GGATG 4 cut(s) 80, 365, 407, 545
FriOI GRGCYC 2 cut(s) 94, 225
Fsp4HI GCNGC 1 cut(s) 149
FspBI CTAG 2 cut(s) 590, 857
GlaI GCGC 3 cut(s) 217, 232, 441
GluI GCNGC 1 cut(s) 149
GsuI CTGGAG 1 cut(s) 554
HaeIII GGCC 5 cut(s) 141, 189, 546, 599, 815
HapII CCGG 3 cut(s) 65, 433, 878
HgaI GACGC 3 cut(s) 17, 83, 189
HhaI GCGC 3 cut(s) 218, 233, 442
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 2 cut(s) 454, 617
Hin6I GCGC 3 cut(s) 216, 231, 440
HinP1I GCGC 3 cut(s) 216, 231, 440
HincII GTYRAC 1 cut(s) 52
HindII GTYRAC 1 cut(s) 52
HindIII AAGCTT 1 cut(s) 665
HinfI GANTC 2 cut(s) 376, 943
HpaII CCGG 3 cut(s) 65, 433, 878
HphI GGTGA 2 cut(s) 259, 716
Hpy166II GTNNAC 5 cut(s) 52, 324, 384, 448, 865
Hpy188I TCNGA 5 cut(s) 7, 156, 212, 243, 484
Hpy188III TCNNGA 7 cut(s) 392, 433, 508, 628, 637, 857, 878
Hpy8I GTNNAC 5 cut(s) 52, 324, 384, 448, 865
Hpy99I CGWCG 4 cut(s) 32, 53, 203, 206
HpyAV CCTTC 2 cut(s) 238, 898
HpyCH4III ACNGT 2 cut(s) 22, 336
HpyCH4IV ACGT 2 cut(s) 204, 861
HpyCH4V TGCA 5 cut(s) 362, 583, 715, 752, 842
HpyF10VI GCNNNNNNNGC 6 cut(s) 368, 557, 571, 580, 664, 848
HpyF3I CTNAG 7 cut(s) 41, 93, 153, 497, 561, 654, 659
HpySE526I ACGT 2 cut(s) 204, 861
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 2 cut(s) 454, 617
HspAI GCGC 3 cut(s) 216, 231, 440
Kpn2I TCCGGA 2 cut(s) 432, 877
LmnI GCTCC 4 cut(s) 33, 89, 220, 880
Lsp1109I GCAGC 1 cut(s) 135
LweI GCATC 4 cut(s) 58, 192, 642, 829
MaeI CTAG 2 cut(s) 590, 857
MaeII ACGT 2 cut(s) 204, 861
MboII GAAGA 7 cut(s) 117, 127, 413, 548, 718, 731, 893
MfeI CAATTG 1 cut(s) 686
MflI RGATCY 1 cut(s) 429
MhlI GDGCHC 3 cut(s) 94, 225, 877
MluCI AATT 6 cut(s) 281, 485, 554, 686, 759, 920
MlyI GAGTC 1 cut(s) 952
MroI TCCGGA 2 cut(s) 432, 877
MroXI GAANNNNTTC 1 cut(s) 380
MseI TTAA 2 cut(s) 17, 83
MspI CCGG 3 cut(s) 65, 433, 878
MspR9I CCNGG 2 cut(s) 236, 602
MunI CAATTG 1 cut(s) 686
Mva1269I GAATGC 2 cut(s) 442, 754
MvaI CCWGG 2 cut(s) 236, 602
MvnI CGCG 3 cut(s) 11, 218, 231
MwoI GCNNNNNNNGC 6 cut(s) 368, 557, 571, 580, 664, 848
NlaIII CATG 2 cut(s) 454, 617
NlaIV GGNNCC 1 cut(s) 35
NmeAIII GCCGAG 2 cut(s) 619, 841
NspI RCATGY 1 cut(s) 617
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PctI GAATGC 2 cut(s) 442, 754
PdmI GAANNNNTTC 1 cut(s) 380
PfeI GAWTC 1 cut(s) 376
PkrI GCNGC 1 cut(s) 150
Ple19I CGATCG 2 cut(s) 229, 273
PleI GAGTC 1 cut(s) 951
PpsI GAGTC 1 cut(s) 951
PpuMI RGGWCCY 1 cut(s) 97
Psp124BI GAGCTC 2 cut(s) 94, 225
Psp5II RGGWCCY 1 cut(s) 97
Psp6I CCWGG 2 cut(s) 234, 600
PspGI CCWGG 2 cut(s) 234, 600
PspN4I GGNNCC 1 cut(s) 35
PspPI GGNCC 4 cut(s) 22, 97, 544, 598
PspPPI RGGWCCY 1 cut(s) 97
PstI CTGCAG 1 cut(s) 585
PstNI CAGNNNCTG 1 cut(s) 241
PsuI RGATCY 1 cut(s) 429
PvuI CGATCG 2 cut(s) 229, 273
RgaI GCGATCGC 1 cut(s) 273
RsaI GTAC 2 cut(s) 260, 323
RsaNI GTAC 2 cut(s) 259, 322
SacI GAGCTC 2 cut(s) 94, 225
SalI GTCGAC 1 cut(s) 50
SaqAI TTAA 2 cut(s) 17, 83
SatI GCNGC 1 cut(s) 149
Sau96I GGNCC 4 cut(s) 22, 97, 544, 598
SbfI CCTGCAGG 1 cut(s) 585
SchI GAGTC 1 cut(s) 952
ScrFI CCNGG 2 cut(s) 236, 602
SdaI CCTGCAGG 1 cut(s) 585
SduI GDGCHC 3 cut(s) 94, 225, 877
SfaAI GCGATCGC 1 cut(s) 273
SfaNI GCATC 4 cut(s) 58, 192, 642, 829
SfcI CTRYAG 1 cut(s) 581
SgfI GCGATCGC 1 cut(s) 273
SinI GGWCC 2 cut(s) 22, 97
SmlI CTYRAG 2 cut(s) 161, 914
SmoI CTYRAG 2 cut(s) 161, 914
Sse8387I CCTGCAGG 1 cut(s) 585
Sse9I AATT 6 cut(s) 281, 485, 554, 686, 759, 920
SsiI CCGC 2 cut(s) 218, 371
SspMI CTAG 2 cut(s) 590, 857
SstI GAGCTC 2 cut(s) 94, 225
StyD4I CCNGG 2 cut(s) 234, 600
TaaI ACNGT 2 cut(s) 22, 336
TaiI ACGT 2 cut(s) 207, 864
TaqI TCGA 5 cut(s) 27, 51, 102, 225, 331
TasI AATT 6 cut(s) 281, 485, 554, 686, 759, 920
TatI WGTACW 1 cut(s) 258
TfiI GAWTC 1 cut(s) 376
Tru1I TTAA 2 cut(s) 17, 83
Tru9I TTAA 2 cut(s) 17, 83
TscAI CASTG 2 cut(s) 391, 849
TseI GCWGC 1 cut(s) 148
TspDTI ATGAA 2 cut(s) 294, 549
TspGWI ACGGA 2 cut(s) 744, 753
TspRI CASTG 2 cut(s) 391, 849
VpaK11BI GGWCC 2 cut(s) 22, 97
XapI RAATTY 2 cut(s) 485, 920
XbaI TCTAGA 1 cut(s) 856
XceI RCATGY 1 cut(s) 617
XmiI GTMKAC 2 cut(s) 51, 864
XmnI GAANNNNTTC 1 cut(s) 380
XspI CTAG 2 cut(s) 590, 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.