AT5G33330

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0018271
Physical Location & Seq
Reverse (-)
12591683 .. 12592904
1222 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G33330.1

Sequence Viewer

Length: 552 bp
ATGGGGAATGTACAAGATATCATGAAAGAAGCCAAAGCATACTGCTGCTTTGTGGATGCATCTTGGGTTTCCCCAACTGAATGTGCAGGAATTGGGTGGGTCCTTTACAATGACAAAGCTCAAATGGTTCTCAAAGGTATGGCCTCTATACCTCCAACTAACTCTTCGATTGAAGCGGAAGCGGAAGCCCTTAGGTCGGCAATGATGCAAGTACGGAGACTGGGTTACGAAGAGGTTAAATTTTGTGTCGATGCTTCAATCCTGTATCAGAATGTAGCGCCATATCTGCAAAAGGACAAGAAGGCATGCCCCACAACTAACTCCCCTTTGGCCGCTTATATCCAGGACATACAAAAACTTGCTACAATTTCAGCTTCTTTTGTTGTAAGTAAAGTGAATAGTACAATCAATGATGTAGCTGATCATCTCGCAAAAGTAGCAAGATCCAAAAAGATGCAATATGTAATATCATGGACTCATGAGTTCAAACAACTCGTGCAGACTACTGAATCGGGTGCAAATGCAAGTCAAGAGGTATCCTCAAATGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-RVT

Protein Analysis

183

Amino Acids

20.07

Weight (kDa)

6.72

Isoelectric Point (pI)

38.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 18 - 146 5.1e-15 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018271)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66045 AT4G03040 AT5G33330
fragaria_vesca FvH4_1g08831 FvH4_1g28351 FvH4_6g21511
malus_domestica MD07G1073700.v1.1
prunus_persica Prupe.5G022500_v2.0.a1 Prupe.6G105300_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0148031

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 176, 182, 333
AclWI GGATC 1 cut(s) 438
AcoI YGGCCR 1 cut(s) 330
AcsI RAATTY 1 cut(s) 239
AfaI GTAC 3 cut(s) 12, 213, 403
AfiI CCNNNNNNNGG 1 cut(s) 196
AgsI TTSAA 3 cut(s) 173, 258, 487
AjnI CCWGG 1 cut(s) 342
AjuI GAANNNNNNNTTGG 2 cut(s) 148, 180
AluBI AGCT 3 cut(s) 119, 374, 419
AluI AGCT 3 cut(s) 119, 374, 419
Alw26I GTCTC 1 cut(s) 211
AlwI GGATC 1 cut(s) 438
AoxI GGCC 2 cut(s) 141, 330
ApeKI GCWGC 1 cut(s) 45
ApoI RAATTY 1 cut(s) 239
AspLEI GCGC 1 cut(s) 280
AspS9I GGNCC 1 cut(s) 100
AvaII GGWCC 1 cut(s) 100
AxyI CCTNAGG 1 cut(s) 191
BauI CACGAG 1 cut(s) 494
BbvI GCAGC 1 cut(s) 32
BciT130I CCWGG 1 cut(s) 344
BciVI GTATCC 1 cut(s) 547
BclI TGATCA 1 cut(s) 421
BcoDI GTCTC 1 cut(s) 211
BfoI RGCGCY 1 cut(s) 281
BfuI GTATCC 1 cut(s) 547
BisI GCNGC 2 cut(s) 46, 333
BlsI GCNGC 2 cut(s) 47, 334
Bme1390I CCNGG 1 cut(s) 344
Bme18I GGWCC 1 cut(s) 100
BmgT120I GGNCC 1 cut(s) 100
BmiI GGNNCC 1 cut(s) 101
BmrFI CCNGG 1 cut(s) 344
BmrI ACTGGG 1 cut(s) 230
BmsI GCATC 5 cut(s) 46, 68, 195, 241, 444
BmuI ACTGGG 1 cut(s) 230
BplI GAGNNNNNCTC 1 cut(s) 524
BsaXI ACNNNNNCTCC 2 cut(s) 208, 238
Bsc4I CCNNNNNNNGG 1 cut(s) 196
Bse1I ACTGG 1 cut(s) 225
Bse21I CCTNAGG 1 cut(s) 191
Bse3DI GCAATG 1 cut(s) 207
BseBI CCWGG 1 cut(s) 344
BseGI GGATG 1 cut(s) 61
BseLI CCNNNNNNNGG 1 cut(s) 196
BseMI GCAATG 1 cut(s) 207
BseNI ACTGG 1 cut(s) 225
BseXI GCAGC 1 cut(s) 32
BsgI GTGCAG 2 cut(s) 105, 518
BshFI GGCC 2 cut(s) 143, 332
BslI CCNNNNNNNGG 1 cut(s) 196
BsmAI GTCTC 1 cut(s) 211
BsnI GGCC 2 cut(s) 143, 332
Bsp1407I TGTACA 1 cut(s) 10
Bsp143I GATC 2 cut(s) 421, 443
BspACI CCGC 3 cut(s) 176, 182, 333
BspANI GGCC 2 cut(s) 143, 332
BspHI TCATGA 2 cut(s) 21, 478
BspLI GGNNCC 1 cut(s) 101
BspPI GGATC 1 cut(s) 438
BsrDI GCAATG 1 cut(s) 207
BsrGI TGTACA 1 cut(s) 10
BsrI ACTGG 1 cut(s) 225
BssMI GATC 2 cut(s) 421, 443
BssSI CACGAG 1 cut(s) 494
Bst2BI CACGAG 1 cut(s) 494
Bst2UI CCWGG 1 cut(s) 344
Bst6I CTCTTC 2 cut(s) 169, 225
BstAUI TGTACA 1 cut(s) 10
BstC8I GCNNGC 1 cut(s) 307
BstDEI CTNAG 1 cut(s) 191
BstF5I GGATG 1 cut(s) 61
BstH2I RGCGCY 1 cut(s) 281
BstHHI GCGC 1 cut(s) 280
BstKTI GATC 2 cut(s) 424, 446
BstMAI GTCTC 1 cut(s) 211
BstMBI GATC 2 cut(s) 421, 443
BstMWI GCNNNNNNNGC 2 cut(s) 286, 437
BstNI CCWGG 1 cut(s) 344
BstNSI RCATGY 1 cut(s) 309
BstSCI CCNGG 1 cut(s) 342
BstV1I GCAGC 1 cut(s) 32
BstX2I RGATCY 1 cut(s) 443
BstYI RGATCY 1 cut(s) 443
Bsu36I CCTNAGG 1 cut(s) 191
BsuI GTATCC 1 cut(s) 547
BsuRI GGCC 2 cut(s) 143, 332
BtsCI GGATG 1 cut(s) 61
Cac8I GCNNGC 1 cut(s) 307
CciI TCATGA 2 cut(s) 21, 478
CfoI GCGC 1 cut(s) 280
Cfr13I GGNCC 1 cut(s) 100
Csp6I GTAC 3 cut(s) 11, 212, 402
CviAII CATG 4 cut(s) 22, 306, 471, 479
CviJI RGCY 7 cut(s) 32, 119, 143, 188, 332, 374, 419
CviKI_1 RGCY 7 cut(s) 32, 119, 143, 188, 332, 374, 419
CviQI GTAC 3 cut(s) 11, 212, 402
DdeI CTNAG 1 cut(s) 191
DpnI GATC 2 cut(s) 423, 445
DpnII GATC 2 cut(s) 421, 443
EaeI YGGCCR 1 cut(s) 330
Eam1104I CTCTTC 2 cut(s) 169, 225
EarI CTCTTC 2 cut(s) 169, 225
Eco32I GATATC 1 cut(s) 19
Eco47I GGWCC 1 cut(s) 100
Eco81I CCTNAGG 1 cut(s) 191
EcoO109I RGGNCCY 1 cut(s) 100
EcoRII CCWGG 1 cut(s) 342
EcoRV GATATC 1 cut(s) 19
EcoT22I ATGCAT 1 cut(s) 61
FaeI CATG 4 cut(s) 25, 309, 474, 482
FatI CATG 4 cut(s) 21, 305, 470, 478
FbaI TGATCA 1 cut(s) 421
Fnu4HI GCNGC 2 cut(s) 46, 333
FokI GGATG 1 cut(s) 68
Fsp4HI GCNGC 2 cut(s) 46, 333
GlaI GCGC 1 cut(s) 279
GluI GCNGC 2 cut(s) 46, 333
HaeII RGCGCY 1 cut(s) 281
HaeIII GGCC 2 cut(s) 143, 332
HhaI GCGC 1 cut(s) 280
Hin1II CATG 4 cut(s) 25, 309, 474, 482
Hin6I GCGC 1 cut(s) 278
HinP1I GCGC 1 cut(s) 278
HinfI GANTC 2 cut(s) 475, 509
Hpy188I TCNGA 1 cut(s) 270
Hpy188III TCNNGA 3 cut(s) 22, 479, 530
HpyAV CCTTC 1 cut(s) 295
HpyCH4V TGCA 8 cut(s) 59, 86, 208, 289, 457, 499, 518, 524
HpyF10VI GCNNNNNNNGC 2 cut(s) 286, 437
HpyF3I CTNAG 1 cut(s) 191
Hsp92II CATG 4 cut(s) 25, 309, 474, 482
HspAI GCGC 1 cut(s) 278
Ksp22I TGATCA 1 cut(s) 421
Kzo9I GATC 2 cut(s) 421, 443
LpnPI CCDG 5 cut(s) 72, 206, 275, 329, 356
Lsp1109I GCAGC 1 cut(s) 32
LweI GCATC 5 cut(s) 46, 68, 195, 241, 444
MaeIII GTNAC 1 cut(s) 224
MalI GATC 2 cut(s) 423, 445
MboI GATC 2 cut(s) 421, 443
MboII GAAGA 2 cut(s) 156, 242
MflI RGATCY 1 cut(s) 443
MluCI AATT 3 cut(s) 90, 239, 366
MlyI GAGTC 1 cut(s) 469
MmeI TCCRAC 1 cut(s) 179
MnlI CCTC 5 cut(s) 154, 162, 226, 526, 550
Mph1103I ATGCAT 1 cut(s) 61
MseI TTAA 2 cut(s) 237, 550
MspR9I CCNGG 1 cut(s) 344
MvaI CCWGG 1 cut(s) 344
MwoI GCNNNNNNNGC 2 cut(s) 286, 437
NdeII GATC 2 cut(s) 421, 443
NlaIII CATG 4 cut(s) 25, 309, 474, 482
NlaIV GGNNCC 1 cut(s) 101
NsiI ATGCAT 1 cut(s) 61
NspI RCATGY 1 cut(s) 309
PaeI GCATGC 1 cut(s) 309
PagI TCATGA 2 cut(s) 21, 478
PfeI GAWTC 1 cut(s) 509
PfoI TCCNGGA 1 cut(s) 342
PkrI GCNGC 2 cut(s) 47, 334
PleI GAGTC 1 cut(s) 469
PpsI GAGTC 1 cut(s) 469
PpuMI RGGWCCY 1 cut(s) 100
Psp5II RGGWCCY 1 cut(s) 100
Psp6I CCWGG 1 cut(s) 342
PspGI CCWGG 1 cut(s) 342
PspN4I GGNNCC 1 cut(s) 101
PspPI GGNCC 1 cut(s) 100
PspPPI RGGWCCY 1 cut(s) 100
PsuI RGATCY 1 cut(s) 443
RsaI GTAC 3 cut(s) 12, 213, 403
RsaNI GTAC 3 cut(s) 11, 212, 402
SaqAI TTAA 2 cut(s) 237, 550
SatI GCNGC 2 cut(s) 46, 333
Sau3AI GATC 2 cut(s) 421, 443
Sau96I GGNCC 1 cut(s) 100
SchI GAGTC 1 cut(s) 469
ScrFI CCNGG 1 cut(s) 344
SetI ASST 8 cut(s) 121, 139, 154, 197, 237, 376, 421, 537
SfaNI GCATC 5 cut(s) 46, 68, 195, 241, 444
SinI GGWCC 1 cut(s) 100
SphI GCATGC 1 cut(s) 309
Sse9I AATT 3 cut(s) 90, 239, 366
SsiI CCGC 3 cut(s) 176, 182, 333
StyD4I CCNGG 1 cut(s) 342
TaqI TCGA 2 cut(s) 167, 249
TasI AATT 3 cut(s) 90, 239, 366
TatI WGTACW 2 cut(s) 10, 401
TauI GCSGC 1 cut(s) 335
TfiI GAWTC 1 cut(s) 509
Tru1I TTAA 2 cut(s) 237, 550
Tru9I TTAA 2 cut(s) 237, 550
TseI GCWGC 1 cut(s) 45
TspDTI ATGAA 1 cut(s) 38
TspGWI ACGGA 1 cut(s) 229
VpaK11BI GGWCC 1 cut(s) 100
XapI RAATTY 1 cut(s) 239
XceI RCATGY 1 cut(s) 309
Zsp2I ATGCAT 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.