AT5G45120

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0017159
Physical Location & Seq
Forward (+)
18241003 .. 18242478
1476 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G45120.1

Sequence Viewer

Length: 1476 bp
ATGGAAACCCAAACACACGTTTTGTTTCTCTTCCTACTCATCACTCTATTGTTAAACACCACCAACAAAACCCAAGCAAGACAACATAAAAACCCTAGTTCCTCCTCATCTTCCTTTCTTGTCCTTACCTTGACCAAATCAAGTGTTTCTCTTCCCACACCAAAGTCTCAAACCCAAGAAAGGATCAAGAAGCCATTATCATCAGTAGATGTTGTAATGGAACCCTTGAGAGAAGTGAGAGATGGTTATTTGATAACCTTAAACATTGGAACACCTCCACAAGCAGTTCAAGTGTACTTGGACACAGGAAGTGATCTCACATGGGTCCCATGTGGGAATCTAAGTTTTGATTGCATTGAATGTTATGATCTCAAGAACAACGATCTAAAATCACCATCGGTCTTTTCTCCTCTTCATTCATCTACATCTTTTAGAGATTCTTGTGCTAGTTCCTTTTGCGTCGAGATCCATAGCTCAGACAATCCTTTTGACCCTTGTGCAGTCGCCGGCTGCTCTGTGAGTATGCTTCTCAAGTCTACTTGCGTAAGGCCTTGTCCTTCCTTTGCTTATACTTATGGTGAAGGTGGTCTTATCTCTGGGATACTAACCAGGGATATACTGAAGGCTCGAACTAGGGATGTTCCAAGGTTTTCTTTTGGCTGCGTAACGTCCACTTACCGGGAACCTATTGGCATTGCTGGATTTGGAAGAGGTTTACTCTCTCTCCCTTCGCAACTAGGCTTTCTTGAAAAGGGATTTTCCCATTGTTTCTTGCCTTTCAAGTTTGTCAACAATCCTAACATCTCTAGCCCTTTGATCCTAGGAGCCTCTGCTTTATCGATAAATCTAACCGACTCTTTGCAATTTACTCCCATGTTGAATACTCCAATGTATCCCAACAGCTACTACATCGGTTTAGAATCAATTACAATAGGCACAAATATCACTCCCACACAAGTTCCTTTAACCTTAAGACAATTTGATTCACAAGGAAATGGAGGAATGTTGGTCGATTCGGGTACGACTTACACACATTTACCCGAACCTTTCTACTCACAACTCCTCACAACTCTCCAATCAACAATCACATACCCAAGAGCCACTGAAACTGAATCAAGAACAGGCTTTGATCTCTGCTACAAAGTCCCGTGTCCTAACAACAATCTCACTAGCCTCGAAAACGATGTAATGATGATATTTCCTTCAATTACATTCCATTTCTTGAACAATGCAACCTTACTTCTCCCGCAAGGTAACTCTTTTTACGCAATGAGCGCCCCTAGCGATGGTTCCGTGGTGCAATGCTTGTTGTTTCAGAACATGGAAGATGGAGACTACGGACCGGCCGGAGTTTTTGGGAGCTTTCAGCAGCAGAATGTGAAAGTTGTGTATGATTTGGAGAAAGAGAGAATTGGCTTCCAAGCTATGGATTGTGTCTTAGAAGCTGCTTCACATGGACTCAACCAAGGCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N TRAM_LAG1_CLN8

Protein Analysis

491

Amino Acids

53.75

Weight (kDa)

5.41

Isoelectric Point (pI)

42.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 83 - 275 4.5e-27 Xylanase inhibitor N-terminal
Asp PF00026 83 - 144 5.8e-08 Eukaryotic aspartyl protease
Asp PF00026 186 - 474 8.6e-06 Eukaryotic aspartyl protease
TAXi_C PF14541 301 - 474 2.5e-29 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017159)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45120
fragaria_vesca FvH4_3g11920
malus_domestica MD05G1256600.v1.1 MD10G1237100.v1.1
prunus_persica Prupe.4G107200_v2.0.a1
pyrus_communis pycom05g23590
rosa_chinensis RchiOBHm_Chr5g0019451
rosa_laevigata RLG00000032444
rosa_roxburghii Rroxscaffold_1G00058610
rosa_rugosa Rorug05G0051000
rosa_samantha Rh5BG140300 Rh5CG152000 Rh5DG140800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1426
AccI GTMKAC 1 cut(s) 536
AciI CCGC 1 cut(s) 1247
AclWI GGATC 3 cut(s) 191, 460, 811
AcoI YGGCCR 1 cut(s) 1344
AcuI CTGAAG 1 cut(s) 641
AfaI GTAC 2 cut(s) 296, 1021
AfiI CCNNNNNNNGG 4 cut(s) 180, 678, 1286, 1426
AflII CTTAAG 1 cut(s) 970
AflIII ACRYGT 1 cut(s) 16
AgsI TTSAA 7 cut(s) 290, 359, 749, 781, 880, 1206, 1225
AjnI CCWGG 1 cut(s) 608
AluBI AGCT 5 cut(s) 474, 903, 1362, 1424, 1445
AluI AGCT 5 cut(s) 474, 903, 1362, 1424, 1445
Alw26I GTCTC 2 cut(s) 171, 1326
AlwI GGATC 3 cut(s) 191, 460, 811
AoxI GGCC 2 cut(s) 548, 1344
ApeKI GCWGC 4 cut(s) 510, 660, 1369, 1445
ArsI GACNNNNNNTTYG 2 cut(s) 470, 502
AspA2I CCTAGG 1 cut(s) 820
AspLEI GCGC 1 cut(s) 1277
AspS9I GGNCC 2 cut(s) 325, 1340
AsuC2I CCSGG 1 cut(s) 680
AsuHPI GGTGA 2 cut(s) 384, 590
AvaII GGWCC 2 cut(s) 325, 1340
AvrII CCTAGG 1 cut(s) 820
BbvI GCAGC 4 cut(s) 497, 647, 1381, 1432
BccI CCATC 4 cut(s) 236, 403, 1280, 1322
BciT130I CCWGG 1 cut(s) 610
BciVI GTATCC 2 cut(s) 594, 903
BcnI CCSGG 1 cut(s) 680
BcoDI GTCTC 2 cut(s) 171, 1326
BfaI CTAG 8 cut(s) 96, 447, 633, 737, 807, 821, 1170, 1281
BfoI RGCGCY 1 cut(s) 1278
BfrI CTTAAG 1 cut(s) 970
BfuI GTATCC 2 cut(s) 594, 903
BisI GCNGC 4 cut(s) 511, 661, 1370, 1446
BlnI CCTAGG 1 cut(s) 820
BlsI GCNGC 4 cut(s) 512, 662, 1371, 1447
Bme1390I CCNGG 2 cut(s) 610, 680
Bme18I GGWCC 2 cut(s) 325, 1340
BmgT120I GGNCC 2 cut(s) 325, 1340
BmiI GGNNCC 6 cut(s) 222, 326, 327, 684, 826, 1291
BmrFI CCNGG 2 cut(s) 610, 680
BplI GAGNNNNNCTC 2 cut(s) 702, 734
BpuEI CTTGAG 3 cut(s) 247, 356, 515
BpuMI CCSGG 1 cut(s) 680
Bsa29I ATCGAT 1 cut(s) 839
BsaJI CCNNGG 5 cut(s) 609, 644, 820, 1293, 1465
BsaXI ACNNNNNCTCC 2 cut(s) 708, 738
Bsc4I CCNNNNNNNGG 4 cut(s) 180, 678, 1286, 1426
Bse118I RCCGGY 2 cut(s) 506, 1342
Bse3DI GCAATG 3 cut(s) 693, 1275, 1307
BseBI CCWGG 1 cut(s) 610
BseCI ATCGAT 1 cut(s) 839
BseDI CCNNGG 5 cut(s) 609, 644, 820, 1293, 1465
BseGI GGATG 1 cut(s) 643
BseLI CCNNNNNNNGG 4 cut(s) 180, 678, 1286, 1426
BseMI GCAATG 3 cut(s) 693, 1275, 1307
BseMII CTCAG 1 cut(s) 489
BseRI GAGGAG 3 cut(s) 94, 399, 1052
BseX3I CGGCCG 1 cut(s) 1344
BseXI GCAGC 4 cut(s) 497, 647, 1381, 1432
BsgI GTGCAG 1 cut(s) 519
Bsh1285I CGRYCG 1 cut(s) 1347
BshFI GGCC 2 cut(s) 550, 1346
BshVI ATCGAT 1 cut(s) 839
BsiEI CGRYCG 1 cut(s) 1347
BsiSI CCGG 4 cut(s) 507, 679, 1343, 1347
BslFI GGGAC 2 cut(s) 311, 1130
BslI CCNNNNNNNGG 4 cut(s) 180, 678, 1286, 1426
BsmAI GTCTC 2 cut(s) 171, 1326
BsmFI GGGAC 2 cut(s) 311, 1130
BsnI GGCC 2 cut(s) 550, 1346
Bsp143I GATC 7 cut(s) 183, 313, 367, 382, 465, 816, 1129
BspACI CCGC 1 cut(s) 1247
BspANI GGCC 2 cut(s) 550, 1346
BspCNI CTCAG 1 cut(s) 488
BspDI ATCGAT 1 cut(s) 839
BspHI TCATGA 1 cut(s) 1472
BspLI GGNNCC 6 cut(s) 222, 326, 327, 684, 826, 1291
BspPI GGATC 3 cut(s) 191, 460, 811
BspTI CTTAAG 1 cut(s) 970
BsrDI GCAATG 3 cut(s) 693, 1275, 1307
BsrFI RCCGGY 2 cut(s) 506, 1342
BssAI RCCGGY 2 cut(s) 506, 1342
BssECI CCNNGG 5 cut(s) 609, 644, 820, 1293, 1465
BssMI GATC 7 cut(s) 183, 313, 367, 382, 465, 816, 1129
BssT1I CCWWGG 3 cut(s) 644, 820, 1465
Bst2UI CCWGG 1 cut(s) 610
Bst6I CTCTTC 4 cut(s) 35, 156, 417, 703
BstAFI CTTAAG 1 cut(s) 970
BstC8I GCNNGC 1 cut(s) 508
BstDEI CTNAG 3 cut(s) 341, 475, 1438
BstDSI CCRYGG 1 cut(s) 1293
BstF5I GGATG 1 cut(s) 643
BstH2I RGCGCY 1 cut(s) 1278
BstHHI GCGC 1 cut(s) 1277
BstKTI GATC 7 cut(s) 186, 316, 370, 385, 468, 819, 1132
BstMAI GTCTC 2 cut(s) 171, 1326
BstMBI GATC 7 cut(s) 183, 313, 367, 382, 465, 816, 1129
BstMCI CGRYCG 1 cut(s) 1347
BstMWI GCNNNNNNNGC 2 cut(s) 1274, 1281
BstNI CCWGG 1 cut(s) 610
BstSCI CCNGG 2 cut(s) 608, 678
BstV1I GCAGC 4 cut(s) 497, 647, 1381, 1432
BstX2I RGATCY 1 cut(s) 465
BstYI RGATCY 1 cut(s) 465
BstZI CGGCCG 1 cut(s) 1344
Bsu15I ATCGAT 1 cut(s) 839
BsuI GTATCC 2 cut(s) 594, 903
BsuRI GGCC 2 cut(s) 550, 1346
BsuTUI ATCGAT 1 cut(s) 839
BtgI CCRYGG 1 cut(s) 1293
BtgZI GCGATG 1 cut(s) 1299
BtsCI GGATG 1 cut(s) 643
BtsIMutI CAGTG 1 cut(s) 1101
Cac8I GCNNGC 1 cut(s) 508
CciI TCATGA 1 cut(s) 1472
CfoI GCGC 1 cut(s) 1277
Cfr10I RCCGGY 2 cut(s) 506, 1342
Cfr13I GGNCC 2 cut(s) 325, 1340
ClaI ATCGAT 1 cut(s) 839
CpoI CGGWCCG 1 cut(s) 1340
CseI GACGC 1 cut(s) 448
Csp6I GTAC 2 cut(s) 295, 1020
CspI CGGWCCG 1 cut(s) 1340
CviAII CATG 6 cut(s) 321, 330, 874, 1321, 1454, 1473
CviQI GTAC 2 cut(s) 295, 1020
DdeI CTNAG 3 cut(s) 341, 475, 1438
DpnI GATC 7 cut(s) 185, 315, 369, 384, 467, 818, 1131
DpnII GATC 7 cut(s) 183, 313, 367, 382, 465, 816, 1129
EaeI YGGCCR 1 cut(s) 1344
EagI CGGCCG 1 cut(s) 1344
Eam1104I CTCTTC 4 cut(s) 35, 156, 417, 703
EarI CTCTTC 4 cut(s) 35, 156, 417, 703
EclXI CGGCCG 1 cut(s) 1344
Eco130I CCWWGG 3 cut(s) 644, 820, 1465
Eco147I AGGCCT 1 cut(s) 550
Eco47I GGWCC 2 cut(s) 325, 1340
Eco52I CGGCCG 1 cut(s) 1344
Eco57I CTGAAG 1 cut(s) 641
EcoO109I RGGNCCY 1 cut(s) 325
EcoRII CCWGG 1 cut(s) 608
EcoT14I CCWWGG 3 cut(s) 644, 820, 1465
ErhI CCWWGG 3 cut(s) 644, 820, 1465
FaeI CATG 6 cut(s) 324, 333, 877, 1324, 1457, 1476
FalI AAGNNNNNCTT 4 cut(s) 573, 605, 637, 669
FaqI GGGAC 2 cut(s) 311, 1130
FatI CATG 6 cut(s) 320, 329, 873, 1320, 1453, 1472
FauI CCCGC 1 cut(s) 1254
FblI GTMKAC 1 cut(s) 536
Fnu4HI GCNGC 4 cut(s) 511, 661, 1370, 1446
FokI GGATG 1 cut(s) 650
Fsp4HI GCNGC 4 cut(s) 511, 661, 1370, 1446
FspBI CTAG 8 cut(s) 96, 447, 633, 737, 807, 821, 1170, 1281
GlaI GCGC 1 cut(s) 1276
GluI GCNGC 4 cut(s) 511, 661, 1370, 1446
HaeII RGCGCY 1 cut(s) 1278
HaeIII GGCC 2 cut(s) 550, 1346
HapII CCGG 4 cut(s) 507, 679, 1343, 1347
HgaI GACGC 1 cut(s) 448
HhaI GCGC 1 cut(s) 1277
Hin1II CATG 6 cut(s) 324, 333, 877, 1324, 1457, 1476
Hin6I GCGC 1 cut(s) 1275
HinP1I GCGC 1 cut(s) 1275
HincII GTYRAC 1 cut(s) 790
HindII GTYRAC 1 cut(s) 790
HinfI GANTC 8 cut(s) 337, 437, 854, 920, 983, 1013, 1112, 1458
HpaII CCGG 4 cut(s) 507, 679, 1343, 1347
HphI GGTGA 2 cut(s) 384, 590
Hpy166II GTNNAC 5 cut(s) 295, 537, 672, 716, 790
Hpy188I TCNGA 2 cut(s) 478, 1317
Hpy188III TCNNGA 7 cut(s) 187, 373, 463, 746, 1116, 1222, 1473
Hpy8I GTNNAC 5 cut(s) 295, 537, 672, 716, 790
Hpy99I CGWCG 1 cut(s) 464
HpyAV CCTTC 5 cut(s) 567, 575, 616, 738, 1212
HpyCH4IV ACGT 2 cut(s) 18, 668
HpyCH4V TGCA 5 cut(s) 354, 500, 862, 1232, 1300
HpyF10VI GCNNNNNNNGC 2 cut(s) 1274, 1281
HpyF3I CTNAG 3 cut(s) 341, 475, 1438
HpySE526I ACGT 2 cut(s) 18, 668
Hsp92II CATG 6 cut(s) 324, 333, 877, 1324, 1457, 1476
HspAI GCGC 1 cut(s) 1275
KflI GGGWCCC 1 cut(s) 325
KroI GCCGGC 1 cut(s) 506
KroNI GCCGGC 1 cut(s) 508
Kzo9I GATC 7 cut(s) 183, 313, 367, 382, 465, 816, 1129
LmnI GCTCC 2 cut(s) 824, 1359
Lsp1109I GCAGC 4 cut(s) 497, 647, 1381, 1432
MaeI CTAG 8 cut(s) 96, 447, 633, 737, 807, 821, 1170, 1281
MaeII ACGT 2 cut(s) 18, 668
MaeIII GTNAC 2 cut(s) 664, 1253
MalI GATC 7 cut(s) 185, 315, 369, 384, 467, 818, 1131
MboI GATC 7 cut(s) 183, 313, 367, 382, 465, 816, 1129
MboII GAAGA 6 cut(s) 22, 102, 143, 404, 720, 1337
MflI RGATCY 1 cut(s) 465
MluCI AATT 5 cut(s) 863, 924, 977, 1206, 1410
MlyI GAGTC 2 cut(s) 848, 1452
MnlI CCTC 9 cut(s) 112, 115, 285, 420, 704, 838, 992, 1073, 1184
MroNI GCCGGC 1 cut(s) 506
MseI TTAA 4 cut(s) 53, 260, 965, 971
MspCI CTTAAG 1 cut(s) 970
MspI CCGG 4 cut(s) 507, 679, 1343, 1347
MspR9I CCNGG 2 cut(s) 610, 680
MvaI CCWGG 1 cut(s) 610
MwoI GCNNNNNNNGC 2 cut(s) 1274, 1281
NaeI GCCGGC 1 cut(s) 508
NciI CCSGG 1 cut(s) 680
NdeII GATC 7 cut(s) 183, 313, 367, 382, 465, 816, 1129
NgoMIV GCCGGC 1 cut(s) 506
NlaIII CATG 6 cut(s) 324, 333, 877, 1324, 1457, 1476
NlaIV GGNNCC 6 cut(s) 222, 326, 327, 684, 826, 1291
PagI TCATGA 1 cut(s) 1472
PceI AGGCCT 1 cut(s) 550
PdiI GCCGGC 1 cut(s) 508
PfeI GAWTC 6 cut(s) 337, 437, 920, 983, 1013, 1112
PflMI CCANNNNNTGG 1 cut(s) 1426
PkrI GCNGC 4 cut(s) 512, 662, 1371, 1447
PleI GAGTC 2 cut(s) 848, 1452
PpsI GAGTC 2 cut(s) 848, 1452
PpuMI RGGWCCY 1 cut(s) 325
Psp5II RGGWCCY 1 cut(s) 325
Psp6I CCWGG 1 cut(s) 608
PspGI CCWGG 1 cut(s) 608
PspN4I GGNNCC 6 cut(s) 222, 326, 327, 684, 826, 1291
PspPI GGNCC 2 cut(s) 325, 1340
PspPPI RGGWCCY 1 cut(s) 325
PsuI RGATCY 1 cut(s) 465
RsaI GTAC 2 cut(s) 296, 1021
RsaNI GTAC 2 cut(s) 295, 1020
Rsr2I CGGWCCG 1 cut(s) 1340
RsrII CGGWCCG 1 cut(s) 1340
SaqAI TTAA 4 cut(s) 53, 260, 965, 971
SatI GCNGC 4 cut(s) 511, 661, 1370, 1446
Sau3AI GATC 7 cut(s) 183, 313, 367, 382, 465, 816, 1129
Sau96I GGNCC 2 cut(s) 325, 1340
SchI GAGTC 2 cut(s) 848, 1452
ScrFI CCNGG 2 cut(s) 610, 680
SinI GGWCC 2 cut(s) 325, 1340
SmlI CTYRAG 4 cut(s) 226, 371, 530, 970
SmoI CTYRAG 4 cut(s) 226, 371, 530, 970
Sse9I AATT 5 cut(s) 863, 924, 977, 1206, 1410
SseBI AGGCCT 1 cut(s) 550
SsiI CCGC 1 cut(s) 1247
SspMI CTAG 8 cut(s) 96, 447, 633, 737, 807, 821, 1170, 1281
StuI AGGCCT 1 cut(s) 550
StyD4I CCNGG 2 cut(s) 608, 678
StyI CCWWGG 3 cut(s) 644, 820, 1465
TaiI ACGT 2 cut(s) 21, 671
TaqI TCGA 5 cut(s) 462, 628, 839, 1011, 1176
TaqII GACCGA 1 cut(s) 388
TasI AATT 5 cut(s) 863, 924, 977, 1206, 1410
TatI WGTACW 1 cut(s) 294
TfiI GAWTC 6 cut(s) 337, 437, 920, 983, 1013, 1112
Tru1I TTAA 4 cut(s) 53, 260, 965, 971
Tru9I TTAA 4 cut(s) 53, 260, 965, 971
TscAI CASTG 1 cut(s) 1108
TseI GCWGC 4 cut(s) 510, 660, 1369, 1445
TspDTI ATGAA 2 cut(s) 404, 408
TspGWI ACGGA 2 cut(s) 1282, 1353
TspRI CASTG 1 cut(s) 1108
Van91I CCANNNNNTGG 1 cut(s) 1426
Vha464I CTTAAG 1 cut(s) 970
VpaK11BI GGWCC 2 cut(s) 325, 1340
XmaJI CCTAGG 1 cut(s) 820
XmiI GTMKAC 1 cut(s) 536
XspI CTAG 8 cut(s) 96, 447, 633, 737, 807, 821, 1170, 1281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.