Rh5BG140300

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0017159
Physical Location & Seq
Reverse (-)
14509758 .. 14511359
1602 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG140300.1

Sequence Viewer

Length: 1254 bp
ATGGAGCCACTGAGGGAAGTCAGAGATGGGTATCTGATATCTCTCAATTTGGGGACACCCCCACAAGTCATTCAAGTTTATATGGATACTGGTAGTGACCTTACTTGGGTTCCTTGTGGAAATCTCTCTTTTAGTTGCATGGATTGTGATGACTATAGAAACACTAAACTAAACCCTACTTTCTCTCCTTCAGCTTCTTCTTCATCACTTAGAGACCTATGTGGTAGCCCCTTTTGCAAAGATATCCATAGCTCCGATAACGCTCTCGATCCATGCACCATGGCCGGTTGCTCACTCACTACCCTTATTAGGGGAACATGTCCTAGACCATGCCCTTCATTTGCTTATACCTATGGTGCGGGTGGGGTTGTCGTAGGGACCCTTAGTAGAGATACACTAAGGGTTCATGGATTTAGTAGTAGTAACCCTAGCAATGTCACTAGTGAAATTCCCAGTTTCTGTTTTGGGTGCATTGGGTCTACTTTTAGAGAGCCAATTGGTATTGTAGGGTTTGGTAGGGGACCGCTTTCCCTCCCATCGCAACTAGGGTTCCTACAAAAGGGCTTCTCTCACTGTTTCTTGGCCTTCAAGTATGTAAACAATCCCAACATTTCAAGCCCTCTAGTTATAGGAGATGTTGCTATTTCTTCTAAAGAAAATATGCAATTCACTCCAATGTTGAAGAGTCCCGTTTACCCTAATTACTACTATATTGGTCTGGAAGCTATCACGATAGGTAATAATAGTATTACACAAGTGCCCTTAAGCTTGAGAGAGTTTGATTCACAAGGTAATGGGGGCATGTTGATTGACTCAGGAACCACTTATACTCATCTTCCTGAGCCATTTTACTCGGACGTTCTTTCAGTGCTCCAATCAGTGATAACATATCCTAGAGCCAAGGAAATGGAGATGAAGACTTCTTTTGATCTTTGTTATAAAGTTCCATACACAACCAATGCTTCCTCAGATGAGCTCTTTCCTTCAATCACTTTCCATTTCTTGAACAATGTAAGCCTTGGATTGCCTCAAGGCAATCACTTCTATGCTATGGGACCTCCAATCAACTCCACCGTGGTGAAATGCTTGTTGTTCCAAACCATGGATGATAGCGATTATGGGCCGGCCGGAGTGTTTGGGAGCTTCCAGCAACAAAATGTGGAGGTCGTGTATGACTTGGAGAAGGAGAGAATTGGGTTTCAAACAATGGACTGTGCTTCAGCTGCAGCCTCTCAAGGCCTTCACAAAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N TRAM_LAG1_CLN8

Protein Analysis

417

Amino Acids

45.47

Weight (kDa)

5.16

Isoelectric Point (pI)

42.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 11 - 212 9.8e-30 Xylanase inhibitor N-terminal
Asp PF00026 11 - 69 7.9e-08 Eukaryotic aspartyl protease
TAXi_C PF14541 236 - 401 1.4e-30 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017159)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45120
fragaria_vesca FvH4_3g11920
malus_domestica MD05G1256600.v1.1 MD10G1237100.v1.1
prunus_persica Prupe.4G107200_v2.0.a1
pyrus_communis pycom05g23590
rosa_chinensis RchiOBHm_Chr5g0019451
rosa_laevigata RLG00000032444
rosa_roxburghii Rroxscaffold_1G00058610
rosa_rugosa Rorug05G0051000
rosa_samantha Rh5BG140300 Rh5CG152000 Rh5DG140800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 939
AccB7I CCANNNNNTGG 1 cut(s) 1102
AccI GTMKAC 1 cut(s) 479
AciI CCGC 2 cut(s) 359, 524
AclWI GGATC 1 cut(s) 263
AcoI YGGCCR 2 cut(s) 282, 1125
AcsI RAATTY 1 cut(s) 447
AcuI CTGAAG 2 cut(s) 174, 1203
AfiI CCNNNNNNNGG 5 cut(s) 106, 309, 310, 559, 1102
AflII CTTAAG 1 cut(s) 763
AflIII ACRYGT 1 cut(s) 317
AgsI TTSAA 7 cut(s) 74, 589, 615, 682, 987, 1006, 1202
AhlI ACTAGT 1 cut(s) 440
AleI CACNNNNGTG 1 cut(s) 1076
AluBI AGCT 7 cut(s) 194, 252, 725, 768, 976, 1143, 1223
AluI AGCT 7 cut(s) 194, 252, 725, 768, 976, 1143, 1223
Alw21I GWGCWC 2 cut(s) 873, 978
Alw26I GTCTC 1 cut(s) 207
AlwI GGATC 1 cut(s) 263
AlwNI CAGNNNCTG 1 cut(s) 459
AoxI GGCC 5 cut(s) 282, 582, 1121, 1125, 1237
ApeKI GCWGC 2 cut(s) 1223, 1226
ApoI RAATTY 1 cut(s) 447
AspS9I GGNCC 4 cut(s) 378, 521, 1055, 1121
AsuHPI GGTGA 1 cut(s) 1090
AvaII GGWCC 3 cut(s) 378, 521, 1055
BaeGI GKGCMC 1 cut(s) 762
BanII GRGCYC 1 cut(s) 978
BbsI GAAGAC 1 cut(s) 923
Bbv12I GWGCWC 2 cut(s) 873, 978
BbvI GCAGC 2 cut(s) 1210, 1238
BccI CCATC 2 cut(s) 20, 544
BciVI GTATCC 1 cut(s) 79
BcoDI GTCTC 1 cut(s) 207
BcuI ACTAGT 1 cut(s) 440
BfaI CTAG 6 cut(s) 324, 429, 441, 545, 623, 894
BfmI CTRYAG 2 cut(s) 154, 1224
BfrI CTTAAG 1 cut(s) 763
BfuI GTATCC 1 cut(s) 79
BisI GCNGC 2 cut(s) 1224, 1227
BlsI GCNGC 2 cut(s) 1225, 1228
Bme18I GGWCC 3 cut(s) 378, 521, 1055
BmgT120I GGNCC 4 cut(s) 378, 521, 1055, 1121
BmiI GGNNCC 8 cut(s) 6, 111, 379, 380, 522, 551, 820, 1056
BmrI ACTGGG 1 cut(s) 447
BmuI ACTGGG 1 cut(s) 447
BpiI GAAGAC 1 cut(s) 923
Bpu10I CCTNAGC 1 cut(s) 840
BpuEI CTTGAG 3 cut(s) 790, 1014, 1218
BsaBI GATNNNNATC 1 cut(s) 30
BsaI GGTCTC 1 cut(s) 207
BsaJI CCNNGG 5 cut(s) 279, 900, 1018, 1074, 1101
BsaXI ACNNNNNCTCC 4 cut(s) 169, 199, 1154, 1184
Bsc4I CCNNNNNNNGG 5 cut(s) 106, 309, 310, 559, 1102
Bse118I RCCGGY 2 cut(s) 284, 1123
Bse1I ACTGG 2 cut(s) 94, 453
Bse3DI GCAATG 1 cut(s) 439
Bse8I GATNNNNATC 1 cut(s) 30
BseDI CCNNGG 5 cut(s) 279, 900, 1018, 1074, 1101
BseGI GGATG 1 cut(s) 1111
BseJI GATNNNNATC 1 cut(s) 30
BseLI CCNNNNNNNGG 5 cut(s) 106, 309, 310, 559, 1102
BseMI GCAATG 1 cut(s) 439
BseMII CTCAG 3 cut(s) 828, 831, 981
BseNI ACTGG 2 cut(s) 94, 453
BseSI GKGCMC 1 cut(s) 762
BseX3I CGGCCG 1 cut(s) 1125
BseXI GCAGC 2 cut(s) 1210, 1238
Bsh1285I CGRYCG 1 cut(s) 1128
BshFI GGCC 5 cut(s) 284, 584, 1123, 1127, 1239
BsiEI CGRYCG 1 cut(s) 1128
BsiHKAI GWGCWC 2 cut(s) 873, 978
BsiSI CCGG 3 cut(s) 285, 1124, 1128
BslFI GGGAC 5 cut(s) 67, 391, 534, 672, 1068
BslI CCNNNNNNNGG 5 cut(s) 106, 309, 310, 559, 1102
BsmAI GTCTC 1 cut(s) 207
BsmFI GGGAC 5 cut(s) 67, 391, 534, 672, 1068
BsnI GGCC 5 cut(s) 284, 584, 1123, 1127, 1239
Bso31I GGTCTC 1 cut(s) 207
Bsp1286I GDGCHC 3 cut(s) 762, 873, 978
Bsp143I GATC 2 cut(s) 268, 928
Bsp19I CCATGG 2 cut(s) 279, 1101
BspACI CCGC 2 cut(s) 359, 524
BspANI GGCC 5 cut(s) 284, 584, 1123, 1127, 1239
BspCNI CTCAG 3 cut(s) 827, 832, 980
BspLI GGNNCC 8 cut(s) 6, 111, 379, 380, 522, 551, 820, 1056
BspMAI CTGCAG 1 cut(s) 1228
BspPI GGATC 1 cut(s) 263
BspTI CTTAAG 1 cut(s) 763
BspTNI GGTCTC 1 cut(s) 207
BsrDI GCAATG 1 cut(s) 439
BsrFI RCCGGY 2 cut(s) 284, 1123
BsrI ACTGG 2 cut(s) 94, 453
BssAI RCCGGY 2 cut(s) 284, 1123
BssECI CCNNGG 5 cut(s) 279, 900, 1018, 1074, 1101
BssMI GATC 2 cut(s) 268, 928
BssT1I CCWWGG 4 cut(s) 279, 900, 1018, 1101
Bst4CI ACNGT 3 cut(s) 575, 1075, 1214
Bst6I CTCTTC 1 cut(s) 677
BstAFI CTTAAG 1 cut(s) 763
BstC8I GCNNGC 1 cut(s) 1125
BstDEI CTNAG 7 cut(s) 11, 209, 383, 398, 814, 840, 967
BstDSI CCRYGG 3 cut(s) 279, 1074, 1101
BstENI CCTNNNNNAGG 1 cut(s) 557
BstF5I GGATG 1 cut(s) 1111
BstKTI GATC 2 cut(s) 271, 931
BstMAI GTCTC 1 cut(s) 207
BstMBI GATC 2 cut(s) 268, 928
BstMCI CGRYCG 1 cut(s) 1128
BstMWI GCNNNNNNNGC 2 cut(s) 234, 1223
BstNSI RCATGY 2 cut(s) 321, 805
BstSFI CTRYAG 2 cut(s) 154, 1224
BstSLI GKGCMC 1 cut(s) 762
BstV1I GCAGC 2 cut(s) 1210, 1238
BstV2I GAAGAC 1 cut(s) 923
BstXI CCANNNNNNTGG 1 cut(s) 907
BstZI CGGCCG 1 cut(s) 1125
BsuI GTATCC 1 cut(s) 79
BsuRI GGCC 5 cut(s) 284, 584, 1123, 1127, 1239
BtgI CCRYGG 3 cut(s) 279, 1074, 1101
BtgZI GCGATG 1 cut(s) 522
BtsCI GGATG 1 cut(s) 1111
BtsIMutI CAGTG 4 cut(s) 8, 571, 873, 885
Cac8I GCNNGC 1 cut(s) 1125
CaiI CAGNNNCTG 1 cut(s) 459
Cfr10I RCCGGY 2 cut(s) 284, 1123
Cfr13I GGNCC 4 cut(s) 378, 521, 1055, 1121
CviAII CATG 8 cut(s) 139, 273, 280, 318, 330, 407, 802, 1102
DdeI CTNAG 7 cut(s) 11, 209, 383, 398, 814, 840, 967
DpnI GATC 2 cut(s) 270, 930
DpnII GATC 2 cut(s) 268, 928
EaeI YGGCCR 2 cut(s) 282, 1125
EagI CGGCCG 1 cut(s) 1125
Eam1104I CTCTTC 1 cut(s) 677
EarI CTCTTC 1 cut(s) 677
Ecl136II GAGCTC 1 cut(s) 976
EclXI CGGCCG 1 cut(s) 1125
Eco130I CCWWGG 4 cut(s) 279, 900, 1018, 1101
Eco147I AGGCCT 1 cut(s) 1239
Eco24I GRGCYC 1 cut(s) 978
Eco31I GGTCTC 1 cut(s) 207
Eco32I GATATC 2 cut(s) 39, 244
Eco47I GGWCC 3 cut(s) 378, 521, 1055
Eco52I CGGCCG 1 cut(s) 1125
Eco53kI GAGCTC 1 cut(s) 976
Eco57I CTGAAG 2 cut(s) 174, 1203
EcoICRI GAGCTC 1 cut(s) 976
EcoNI CCTNNNNNAGG 1 cut(s) 557
EcoO109I RGGNCCY 2 cut(s) 378, 1055
EcoRV GATATC 2 cut(s) 39, 244
EcoT14I CCWWGG 4 cut(s) 279, 900, 1018, 1101
EcoT38I GRGCYC 1 cut(s) 978
ErhI CCWWGG 4 cut(s) 279, 900, 1018, 1101
FaeI CATG 8 cut(s) 142, 276, 283, 321, 333, 410, 805, 1105
FaqI GGGAC 5 cut(s) 67, 391, 534, 672, 1068
FatI CATG 8 cut(s) 138, 272, 279, 317, 329, 406, 801, 1101
FauI CCCGC 1 cut(s) 352
FblI GTMKAC 1 cut(s) 479
Fnu4HI GCNGC 2 cut(s) 1224, 1227
FokI GGATG 1 cut(s) 1118
FriOI GRGCYC 1 cut(s) 978
FseI GGCCGGCC 1 cut(s) 1127
Fsp4HI GCNGC 2 cut(s) 1224, 1227
FspBI CTAG 6 cut(s) 324, 429, 441, 545, 623, 894
GluI GCNGC 2 cut(s) 1224, 1227
HaeIII GGCC 5 cut(s) 284, 584, 1123, 1127, 1239
HapII CCGG 3 cut(s) 285, 1124, 1128
Hin1II CATG 8 cut(s) 142, 276, 283, 321, 333, 410, 805, 1105
HindIII AAGCTT 1 cut(s) 766
HinfI GANTC 3 cut(s) 685, 782, 812
HpaII CCGG 3 cut(s) 285, 1124, 1128
HphI GGTGA 1 cut(s) 1090
Hpy166II GTNNAC 3 cut(s) 480, 598, 694
Hpy188I TCNGA 5 cut(s) 23, 36, 256, 856, 970
Hpy188III TCNNGA 6 cut(s) 266, 719, 730, 816, 839, 1003
Hpy8I GTNNAC 3 cut(s) 480, 598, 694
HpyAV CCTTC 6 cut(s) 198, 345, 595, 993, 1177, 1250
HpyCH4III ACNGT 3 cut(s) 575, 1075, 1214
HpyCH4IV ACGT 1 cut(s) 858
HpyCH4V TGCA 6 cut(s) 138, 237, 276, 471, 664, 1226
HpyF10VI GCNNNNNNNGC 2 cut(s) 234, 1223
HpyF3I CTNAG 7 cut(s) 11, 209, 383, 398, 814, 840, 967
HpySE526I ACGT 1 cut(s) 858
Hsp92II CATG 8 cut(s) 142, 276, 283, 321, 333, 410, 805, 1105
KflI GGGWCCC 1 cut(s) 378
KroI GCCGGC 1 cut(s) 1123
KroNI GCCGGC 1 cut(s) 1125
Kzo9I GATC 2 cut(s) 268, 928
LmnI GCTCC 4 cut(s) 4, 257, 876, 1140
LpnPI CCDG 9 cut(s) 75, 298, 466, 704, 801, 852, 1137, 1141, 1160
Lsp1109I GCAGC 2 cut(s) 1210, 1238
MaeI CTAG 6 cut(s) 324, 429, 441, 545, 623, 894
MaeII ACGT 1 cut(s) 858
MaeIII GTNAC 3 cut(s) 95, 422, 436
MalI GATC 2 cut(s) 270, 930
MboI GATC 2 cut(s) 268, 928
MboII GAAGA 6 cut(s) 189, 192, 639, 694, 827, 928
MfeI CAATTG 1 cut(s) 495
MhlI GDGCHC 3 cut(s) 762, 873, 978
MluCI AATT 6 cut(s) 46, 447, 495, 665, 700, 1191
MlyI GAGTC 2 cut(s) 694, 806
MnlI CCTC 8 cut(s) 6, 542, 630, 976, 1038, 1068, 1156, 1240
MroNI GCCGGC 1 cut(s) 1123
MseI TTAA 1 cut(s) 764
MslI CAYNNNNRTG 3 cut(s) 674, 1044, 1076
MspA1I CMGCKG 1 cut(s) 1223
MspCI CTTAAG 1 cut(s) 763
MspI CCGG 3 cut(s) 285, 1124, 1128
MunI CAATTG 1 cut(s) 495
MwoI GCNNNNNNNGC 2 cut(s) 234, 1223
NaeI GCCGGC 1 cut(s) 1125
NcoI CCATGG 2 cut(s) 279, 1101
NdeII GATC 2 cut(s) 268, 928
NgoMIV GCCGGC 1 cut(s) 1123
NlaIII CATG 8 cut(s) 142, 276, 283, 321, 333, 410, 805, 1105
NlaIV GGNNCC 8 cut(s) 6, 111, 379, 380, 522, 551, 820, 1056
NmuCI GTSAC 2 cut(s) 95, 436
NspI RCATGY 2 cut(s) 321, 805
OliI CACNNNNGTG 1 cut(s) 1076
PceI AGGCCT 1 cut(s) 1239
PciI ACATGT 1 cut(s) 317
PdiI GCCGGC 1 cut(s) 1125
PfeI GAWTC 1 cut(s) 782
PflMI CCANNNNNTGG 1 cut(s) 1102
PkrI GCNGC 2 cut(s) 1225, 1228
PleI GAGTC 2 cut(s) 693, 806
PpsI GAGTC 2 cut(s) 693, 806
PpuMI RGGWCCY 2 cut(s) 378, 1055
PscI ACATGT 1 cut(s) 317
PsiI TTATAA 1 cut(s) 939
Psp124BI GAGCTC 1 cut(s) 978
Psp5II RGGWCCY 2 cut(s) 378, 1055
PspN4I GGNNCC 8 cut(s) 6, 111, 379, 380, 522, 551, 820, 1056
PspPI GGNCC 4 cut(s) 378, 521, 1055, 1121
PspPPI RGGWCCY 2 cut(s) 378, 1055
PsrI GAACNNNNNNTAC 2 cut(s) 811, 843
PstI CTGCAG 1 cut(s) 1228
PstNI CAGNNNCTG 1 cut(s) 459
PvuII CAGCTG 1 cut(s) 1223
RigI GGCCGGCC 1 cut(s) 1127
RseI CAYNNNNRTG 3 cut(s) 674, 1044, 1076
SacI GAGCTC 1 cut(s) 978
SaqAI TTAA 1 cut(s) 764
SatI GCNGC 2 cut(s) 1224, 1227
Sau3AI GATC 2 cut(s) 268, 928
Sau96I GGNCC 4 cut(s) 378, 521, 1055, 1121
SchI GAGTC 2 cut(s) 694, 806
SduI GDGCHC 3 cut(s) 762, 873, 978
SfcI CTRYAG 2 cut(s) 154, 1224
SinI GGWCC 3 cut(s) 378, 521, 1055
SmiMI CAYNNNNRTG 3 cut(s) 674, 1044, 1076
SmlI CTYRAG 4 cut(s) 763, 769, 1029, 1233
SmoI CTYRAG 4 cut(s) 763, 769, 1029, 1233
SpeI ACTAGT 1 cut(s) 440
Sse9I AATT 6 cut(s) 46, 447, 495, 665, 700, 1191
SseBI AGGCCT 1 cut(s) 1239
SsiI CCGC 2 cut(s) 359, 524
SspMI CTAG 6 cut(s) 324, 429, 441, 545, 623, 894
SstI GAGCTC 1 cut(s) 978
StuI AGGCCT 1 cut(s) 1239
StyI CCWWGG 4 cut(s) 279, 900, 1018, 1101
TaaI ACNGT 3 cut(s) 575, 1075, 1214
TaiI ACGT 1 cut(s) 861
TaqI TCGA 1 cut(s) 267
TasI AATT 6 cut(s) 46, 447, 495, 665, 700, 1191
TfiI GAWTC 1 cut(s) 782
Tru1I TTAA 1 cut(s) 764
Tru9I TTAA 1 cut(s) 764
TscAI CASTG 4 cut(s) 15, 578, 873, 885
TseFI GTSAC 2 cut(s) 95, 436
TseI GCWGC 2 cut(s) 1223, 1226
Tsp45I GTSAC 2 cut(s) 95, 436
TspDTI ATGAA 4 cut(s) 192, 327, 395, 929
TspRI CASTG 4 cut(s) 15, 578, 873, 885
Van91I CCANNNNNTGG 1 cut(s) 1102
Vha464I CTTAAG 1 cut(s) 763
VpaK11BI GGWCC 3 cut(s) 378, 521, 1055
XagI CCTNNNNNAGG 1 cut(s) 557
XapI RAATTY 1 cut(s) 447
XceI RCATGY 2 cut(s) 321, 805
XmiI GTMKAC 1 cut(s) 479
XspI CTAG 6 cut(s) 324, 429, 441, 545, 623, 894
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.