Belongs to the peptidase A1 family
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT5G45120 |
| fragaria_vesca | FvH4_3g11920 |
| malus_domestica | MD05G1256600.v1.1 MD10G1237100.v1.1 |
| prunus_persica | Prupe.4G107200_v2.0.a1 |
| pyrus_communis | pycom05g23590 |
| rosa_chinensis | RchiOBHm_Chr5g0019451 |
| rosa_laevigata | RLG00000032444 |
| rosa_roxburghii | Rroxscaffold_1G00058610 |
| rosa_rugosa | Rorug05G0051000 |
| rosa_samantha | Rh5BG140300 Rh5CG152000 Rh5DG140800 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 2274 |
| Acc36I | ACCTGC | 1 cut(s) | 2458 |
| AccB1I | GGYRCC | 1 cut(s) | 2642 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1882 |
| AccII | CGCG | 1 cut(s) | 3097 |
| AciI | CCGC | 2 cut(s) | 204, 381 |
| AclWI | GGATC | 4 cut(s) | 556, 1028, 2205, 2326 |
| AcoI | YGGCCR | 2 cut(s) | 939, 2419 |
| AcsI | RAATTY | 9 cut(s) | 139, 261, 628, 1212, 2188, 2405, 2708, 2977, 3104 |
| AcuI | CTGAAG | 1 cut(s) | 939 |
| AfaI | GTAC | 6 cut(s) | 1460, 1513, 2654, 2964, 2998, 3118 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 871, 1018, 1882, 2181, 2456, 2559, 2967 |
| AflIII | ACRYGT | 2 cut(s) | 1082, 2195 |
| AhlI | ACTAGT | 1 cut(s) | 1508 |
| AjnI | CCWGG | 2 cut(s) | 672, 1743 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1299, 1331 |
| AluBI | AGCT | 8 cut(s) | 154, 959, 993, 1017, 1536, 1838, 1907, 1923 |
| AluI | AGCT | 8 cut(s) | 154, 959, 993, 1017, 1536, 1838, 1907, 1923 |
| Alw21I | GWGCWC | 3 cut(s) | 1043, 1291, 1840 |
| Alw26I | GTCTC | 5 cut(s) | 250, 552, 972, 1149, 1673 |
| Alw44I | GTGCAC | 1 cut(s) | 1039 |
| AlwI | GGATC | 4 cut(s) | 556, 1028, 2205, 2326 |
| Ama87I | CYCGRG | 1 cut(s) | 696 |
| AoxI | GGCC | 5 cut(s) | 939, 1347, 1488, 2419, 2863 |
| ApaLI | GTGCAC | 1 cut(s) | 1039 |
| ApeKI | GCWGC | 2 cut(s) | 2057, 2609 |
| ApoI | RAATTY | 9 cut(s) | 139, 261, 628, 1212, 2188, 2405, 2708, 2977, 3104 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1786, 1818 |
| AseI | ATTAAT | 3 cut(s) | 233, 462, 2372 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1328 |
| AspLEI | GCGC | 1 cut(s) | 1124 |
| AspS9I | GGNCC | 1 cut(s) | 1901 |
| AsuC2I | CCSGG | 1 cut(s) | 3054 |
| AsuHPI | GGTGA | 5 cut(s) | 574, 625, 1660, 1870, 2413 |
| AsuII | TTCGAA | 2 cut(s) | 259, 2690 |
| AvaI | CYCGRG | 1 cut(s) | 696 |
| AvaII | GGWCC | 1 cut(s) | 1901 |
| BaeGI | GKGCMC | 3 cut(s) | 1043, 1147, 1530 |
| BalI | TGGCCA | 2 cut(s) | 941, 2421 |
| BanI | GGYRCC | 1 cut(s) | 2642 |
| BanII | GRGCYC | 1 cut(s) | 1840 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 3141, 3173 |
| BauI | CACGAG | 1 cut(s) | 2696 |
| Bbv12I | GWGCWC | 3 cut(s) | 1043, 1291, 1840 |
| BbvI | GCAGC | 2 cut(s) | 2069, 2596 |
| BccI | CCATC | 9 cut(s) | 52, 660, 754, 785, 1037, 1499, 1883, 2012, 2391 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 724, 758, 1030, 1064 |
| BciT130I | CCWGG | 2 cut(s) | 674, 1745 |
| BciVI | GTATCC | 3 cut(s) | 844, 1668, 2453 |
| BcnI | CCSGG | 1 cut(s) | 3054 |
| BcoDI | GTCTC | 5 cut(s) | 250, 552, 972, 1149, 1673 |
| BcuI | ACTAGT | 1 cut(s) | 1508 |
| BfmI | CTRYAG | 3 cut(s) | 919, 1319, 2656 |
| BfuAI | ACCTGC | 1 cut(s) | 2458 |
| BfuI | GTATCC | 3 cut(s) | 844, 1668, 2453 |
| BglII | AGATCT | 1 cut(s) | 2924 |
| BisI | GCNGC | 2 cut(s) | 2058, 2610 |
| BlsI | GCNGC | 2 cut(s) | 2059, 2611 |
| BmcAI | AGTACT | 1 cut(s) | 2654 |
| Bme1390I | CCNGG | 3 cut(s) | 674, 1745, 3054 |
| Bme18I | GGWCC | 1 cut(s) | 1901 |
| BmeT110I | CYCGRG | 1 cut(s) | 696 |
| BmgT120I | GGNCC | 1 cut(s) | 1901 |
| BmiI | GGNNCC | 3 cut(s) | 876, 1588, 2644 |
| BmrFI | CCNGG | 3 cut(s) | 674, 1745, 3054 |
| BmrI | ACTGGG | 1 cut(s) | 864 |
| BmsI | GCATC | 4 cut(s) | 731, 732, 2317, 2761 |
| BmuI | ACTGGG | 1 cut(s) | 864 |
| BpmI | CTGGAG | 2 cut(s) | 1824, 1834 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1608 |
| Bpu14I | TTCGAA | 2 cut(s) | 259, 2690 |
| BpuEI | CTTGAG | 2 cut(s) | 1516, 1558 |
| BpuMI | CCSGG | 1 cut(s) | 3054 |
| Bsa29I | ATCGAT | 1 cut(s) | 1032 |
| BsaBI | GATNNNNATC | 1 cut(s) | 795 |
| BsaI | GGTCTC | 1 cut(s) | 972 |
| BsaJI | CCNNGG | 8 cut(s) | 672, 1138, 1668, 1810, 1830, 2558, 2966, 3053 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1934, 1964 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 871, 1018, 1882, 2181, 2456, 2559, 2967 |
| Bse1I | ACTGG | 2 cut(s) | 859, 1851 |
| Bse3DI | GCAATG | 2 cut(s) | 1336, 1567 |
| Bse8I | GATNNNNATC | 1 cut(s) | 795 |
| BseBI | CCWGG | 2 cut(s) | 674, 1745 |
| BseCI | ATCGAT | 1 cut(s) | 1032 |
| BseDI | CCNNGG | 8 cut(s) | 672, 1138, 1668, 1810, 1830, 2558, 2966, 3053 |
| BseGI | GGATG | 6 cut(s) | 417, 1008, 1058, 1753, 1891, 3038 |
| BseJI | GATNNNNATC | 1 cut(s) | 795 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 871, 1018, 1882, 2181, 2456, 2559, 2967 |
| BseMI | GCAATG | 2 cut(s) | 1336, 1567 |
| BseMII | CTCAG | 5 cut(s) | 767, 1596, 1599, 2663, 2907 |
| BseNI | ACTGG | 2 cut(s) | 859, 1851 |
| BseSI | GKGCMC | 3 cut(s) | 1043, 1147, 1530 |
| BseXI | GCAGC | 2 cut(s) | 2069, 2596 |
| BseYI | CCCAGC | 1 cut(s) | 1907 |
| BsgI | GTGCAG | 1 cut(s) | 2436 |
| Bsh1236I | CGCG | 1 cut(s) | 3097 |
| BshFI | GGCC | 5 cut(s) | 941, 1349, 1490, 2421, 2865 |
| BshNI | GGYRCC | 1 cut(s) | 2642 |
| BshVI | ATCGAT | 1 cut(s) | 1032 |
| BsiHKAI | GWGCWC | 3 cut(s) | 1043, 1291, 1840 |
| BsiHKCI | CYCGRG | 1 cut(s) | 696 |
| BsiSI | CCGG | 1 cut(s) | 3053 |
| BslFI | GGGAC | 5 cut(s) | 832, 1437, 1697, 2540, 3073 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 871, 1018, 1882, 2181, 2456, 2559, 2967 |
| BsmAI | GTCTC | 5 cut(s) | 250, 552, 972, 1149, 1673 |
| BsmBI | CGTCTC | 1 cut(s) | 250 |
| BsmFI | GGGAC | 5 cut(s) | 832, 1437, 1697, 2540, 3073 |
| BsnI | GGCC | 5 cut(s) | 941, 1349, 1490, 2421, 2865 |
| Bso31I | GGTCTC | 1 cut(s) | 972 |
| BsoBI | CYCGRG | 1 cut(s) | 696 |
| Bsp119I | TTCGAA | 2 cut(s) | 259, 2690 |
| Bsp1286I | GDGCHC | 5 cut(s) | 1043, 1147, 1291, 1530, 1840 |
| Bsp143I | GATC | 9 cut(s) | 24, 478, 561, 765, 1033, 1696, 2210, 2331, 2924 |
| Bsp19I | CCATGG | 3 cut(s) | 1830, 2558, 2966 |
| BspACI | CCGC | 2 cut(s) | 204, 381 |
| BspANI | GGCC | 5 cut(s) | 941, 1349, 1490, 2421, 2865 |
| BspCNI | CTCAG | 5 cut(s) | 768, 1595, 1600, 2662, 2908 |
| BspDI | ATCGAT | 1 cut(s) | 1032 |
| BspFNI | CGCG | 1 cut(s) | 3097 |
| BspLI | GGNNCC | 3 cut(s) | 876, 1588, 2644 |
| BspMI | ACCTGC | 1 cut(s) | 2458 |
| BspPI | GGATC | 4 cut(s) | 556, 1028, 2205, 2326 |
| BspT104I | TTCGAA | 2 cut(s) | 259, 2690 |
| BspT107I | GGYRCC | 1 cut(s) | 2642 |
| BspTNI | GGTCTC | 1 cut(s) | 972 |
| BsrDI | GCAATG | 2 cut(s) | 1336, 1567 |
| BsrI | ACTGG | 2 cut(s) | 859, 1851 |
| BssECI | CCNNGG | 8 cut(s) | 672, 1138, 1668, 1810, 1830, 2558, 2966, 3053 |
| BssMI | GATC | 9 cut(s) | 24, 478, 561, 765, 1033, 1696, 2210, 2331, 2924 |
| BssSI | CACGAG | 1 cut(s) | 2696 |
| BssT1I | CCWWGG | 6 cut(s) | 1138, 1668, 1810, 1830, 2558, 2966 |
| Bst2BI | CACGAG | 1 cut(s) | 2696 |
| Bst2UI | CCWGG | 2 cut(s) | 674, 1745 |
| Bst4CI | ACNGT | 4 cut(s) | 1516, 2660, 2953, 2962 |
| Bst6I | CTCTTC | 4 cut(s) | 1442, 2681, 2872, 3127 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 2606 |
| BstBI | TTCGAA | 2 cut(s) | 259, 2690 |
| BstC8I | GCNNGC | 7 cut(s) | 402, 406, 410, 433, 529, 649, 1571 |
| BstDEI | CTNAG | 9 cut(s) | 776, 809, 974, 1148, 1582, 1608, 2180, 2649, 2916 |
| BstDSI | CCRYGG | 3 cut(s) | 1830, 2558, 2966 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 2179, 2454 |
| BstF5I | GGATG | 6 cut(s) | 417, 1008, 1058, 1753, 1891, 3038 |
| BstFNI | CGCG | 1 cut(s) | 3097 |
| BstHHI | GCGC | 1 cut(s) | 1124 |
| BstKTI | GATC | 9 cut(s) | 27, 481, 564, 768, 1036, 1699, 2213, 2334, 2927 |
| BstMAI | GTCTC | 5 cut(s) | 250, 552, 972, 1149, 1673 |
| BstMBI | GATC | 9 cut(s) | 24, 478, 561, 765, 1033, 1696, 2210, 2331, 2924 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 999, 1566, 1835, 2606 |
| BstNI | CCWGG | 2 cut(s) | 674, 1745 |
| BstNSI | RCATGY | 7 cut(s) | 404, 408, 412, 531, 1086, 1573, 2199 |
| BstSCI | CCNGG | 3 cut(s) | 672, 1743, 3052 |
| BstSFI | CTRYAG | 3 cut(s) | 919, 1319, 2656 |
| BstSLI | GKGCMC | 3 cut(s) | 1043, 1147, 1530 |
| BstUI | CGCG | 1 cut(s) | 3097 |
| BstV1I | GCAGC | 2 cut(s) | 2069, 2596 |
| BstX2I | RGATCY | 2 cut(s) | 2210, 2924 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1675 |
| BstYI | RGATCY | 2 cut(s) | 2210, 2924 |
| Bsu15I | ATCGAT | 1 cut(s) | 1032 |
| BsuI | GTATCC | 3 cut(s) | 844, 1668, 2453 |
| BsuRI | GGCC | 5 cut(s) | 941, 1349, 1490, 2421, 2865 |
| BsuTUI | ATCGAT | 1 cut(s) | 1032 |
| BtgI | CCRYGG | 3 cut(s) | 1830, 2558, 2966 |
| BtgZI | GCGATG | 1 cut(s) | 638 |
| BtsCI | GGATG | 6 cut(s) | 417, 1008, 1058, 1753, 1891, 3038 |
| BtsIMutI | CAGTG | 2 cut(s) | 773, 1858 |
| BveI | ACCTGC | 1 cut(s) | 2458 |
| Cac8I | GCNNGC | 7 cut(s) | 402, 406, 410, 433, 529, 649, 1571 |
| CfoI | GCGC | 1 cut(s) | 1124 |
| Cfr13I | GGNCC | 1 cut(s) | 1901 |
| ClaI | ATCGAT | 1 cut(s) | 1032 |
| CseI | GACGC | 1 cut(s) | 2487 |
| Csp6I | GTAC | 6 cut(s) | 1459, 1512, 2653, 2963, 2997, 3117 |
| CviQI | GTAC | 6 cut(s) | 1459, 1512, 2653, 2963, 2997, 3117 |
| DdeI | CTNAG | 9 cut(s) | 776, 809, 974, 1148, 1582, 1608, 2180, 2649, 2916 |
| DpnI | GATC | 9 cut(s) | 26, 480, 563, 767, 1035, 1698, 2212, 2333, 2926 |
| DpnII | GATC | 9 cut(s) | 24, 478, 561, 765, 1033, 1696, 2210, 2331, 2924 |
| DraI | TTTAAA | 1 cut(s) | 138 |
| EaeI | YGGCCR | 2 cut(s) | 939, 2419 |
| Eam1104I | CTCTTC | 4 cut(s) | 1442, 2681, 2872, 3127 |
| EarI | CTCTTC | 4 cut(s) | 1442, 2681, 2872, 3127 |
| Ecl136II | GAGCTC | 1 cut(s) | 1838 |
| Eco130I | CCWWGG | 6 cut(s) | 1138, 1668, 1810, 1830, 2558, 2966 |
| Eco147I | AGGCCT | 1 cut(s) | 2865 |
| Eco24I | GRGCYC | 1 cut(s) | 1840 |
| Eco31I | GGTCTC | 1 cut(s) | 972 |
| Eco47I | GGWCC | 1 cut(s) | 1901 |
| Eco53kI | GAGCTC | 1 cut(s) | 1838 |
| Eco57I | CTGAAG | 1 cut(s) | 939 |
| Eco88I | CYCGRG | 1 cut(s) | 696 |
| EcoICRI | GAGCTC | 1 cut(s) | 1838 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 2179, 2454 |
| EcoRI | GAATTC | 1 cut(s) | 2708 |
| EcoRII | CCWGG | 2 cut(s) | 672, 1743 |
| EcoT14I | CCWWGG | 6 cut(s) | 1138, 1668, 1810, 1830, 2558, 2966 |
| EcoT22I | ATGCAT | 3 cut(s) | 406, 410, 529 |
| EcoT38I | GRGCYC | 1 cut(s) | 1840 |
| ErhI | CCWWGG | 6 cut(s) | 1138, 1668, 1810, 1830, 2558, 2966 |
| Esp3I | CGTCTC | 1 cut(s) | 250 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1131, 1163 |
| FaqI | GGGAC | 5 cut(s) | 832, 1437, 1697, 2540, 3073 |
| FauI | CCCGC | 1 cut(s) | 374 |
| FauNDI | CATATG | 2 cut(s) | 54, 501 |
| Fnu4HI | GCNGC | 2 cut(s) | 2058, 2610 |
| FokI | GGATG | 6 cut(s) | 404, 995, 1065, 1760, 1898, 3045 |
| FriOI | GRGCYC | 1 cut(s) | 1840 |
| Fsp4HI | GCNGC | 2 cut(s) | 2058, 2610 |
| GlaI | GCGC | 1 cut(s) | 1123 |
| GluI | GCNGC | 2 cut(s) | 2058, 2610 |
| GsaI | CCCAGC | 1 cut(s) | 1911 |
| GsuI | CTGGAG | 2 cut(s) | 1824, 1834 |
| HaeIII | GGCC | 5 cut(s) | 941, 1349, 1490, 2421, 2865 |
| HapII | CCGG | 1 cut(s) | 3053 |
| HgaI | GACGC | 1 cut(s) | 2487 |
| HhaI | GCGC | 1 cut(s) | 1124 |
| Hin6I | GCGC | 1 cut(s) | 1122 |
| HinP1I | GCGC | 1 cut(s) | 1122 |
| HindIII | AAGCTT | 1 cut(s) | 1534 |
| HpaII | CCGG | 1 cut(s) | 3053 |
| HphI | GGTGA | 5 cut(s) | 574, 625, 1660, 1870, 2413 |
| Hpy166II | GTNNAC | 9 cut(s) | 120, 132, 190, 369, 541, 1041, 1058, 2447, 3067 |
| Hpy188I | TCNGA | 7 cut(s) | 397, 469, 717, 760, 801, 1021, 1052 |
| Hpy188III | TCNNGA | 5 cut(s) | 476, 625, 1484, 1584, 1783 |
| Hpy8I | GTNNAC | 9 cut(s) | 120, 132, 190, 369, 541, 1041, 1058, 2447, 3067 |
| Hpy99I | CGWCG | 2 cut(s) | 2219, 3048 |
| HpyCH4III | ACNGT | 4 cut(s) | 1516, 2660, 2953, 2962 |
| HpyCH4IV | ACGT | 1 cut(s) | 371 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 999, 1566, 1835, 2606 |
| HpyF3I | CTNAG | 9 cut(s) | 776, 809, 974, 1148, 1582, 1608, 2180, 2649, 2916 |
| HpySE526I | ACGT | 1 cut(s) | 371 |
| HspAI | GCGC | 1 cut(s) | 1122 |
| Kzo9I | GATC | 9 cut(s) | 24, 478, 561, 765, 1033, 1696, 2210, 2331, 2924 |
| LmnI | GCTCC | 5 cut(s) | 662, 1286, 1835, 1843, 1920 |
| Lsp1109I | GCAGC | 2 cut(s) | 2069, 2596 |
| LweI | GCATC | 4 cut(s) | 731, 732, 2317, 2761 |
| MaeII | ACGT | 1 cut(s) | 371 |
| MaeIII | GTNAC | 7 cut(s) | 492, 860, 1201, 1516, 1792, 2101, 2365 |
| MalI | GATC | 9 cut(s) | 26, 480, 563, 767, 1035, 1698, 2212, 2333, 2926 |
| MboI | GATC | 9 cut(s) | 24, 478, 561, 765, 1033, 1696, 2210, 2331, 2924 |
| MflI | RGATCY | 2 cut(s) | 2210, 2924 |
| MhlI | GDGCHC | 5 cut(s) | 1043, 1147, 1291, 1530, 1840 |
| MlsI | TGGCCA | 2 cut(s) | 941, 2421 |
| MluNI | TGGCCA | 2 cut(s) | 941, 2421 |
| MlyI | GAGTC | 6 cut(s) | 814, 1459, 1574, 1652, 1789, 3095 |
| MmeI | TCCRAC | 3 cut(s) | 1030, 1647, 1951 |
| Mox20I | TGGCCA | 2 cut(s) | 941, 2421 |
| Mph1103I | ATGCAT | 3 cut(s) | 406, 410, 529 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1328 |
| MscI | TGGCCA | 2 cut(s) | 941, 2421 |
| MslI | CAYNNNNRTG | 5 cut(s) | 1439, 1560, 1824, 2468, 2640 |
| Msp20I | TGGCCA | 2 cut(s) | 941, 2421 |
| MspA1I | CMGCKG | 1 cut(s) | 1907 |
| MspI | CCGG | 1 cut(s) | 3053 |
| MspR9I | CCNGG | 3 cut(s) | 674, 1745, 3054 |
| MvaI | CCWGG | 2 cut(s) | 674, 1745 |
| MvnI | CGCG | 1 cut(s) | 3097 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 999, 1566, 1835, 2606 |
| NciI | CCSGG | 1 cut(s) | 3054 |
| NcoI | CCATGG | 3 cut(s) | 1830, 2558, 2966 |
| NdeI | CATATG | 2 cut(s) | 54, 501 |
| NdeII | GATC | 9 cut(s) | 24, 478, 561, 765, 1033, 1696, 2210, 2331, 2924 |
| NlaIV | GGNNCC | 3 cut(s) | 876, 1588, 2644 |
| NmuCI | GTSAC | 3 cut(s) | 860, 1201, 1792 |
| NsiI | ATGCAT | 3 cut(s) | 406, 410, 529 |
| NspI | RCATGY | 7 cut(s) | 404, 408, 412, 531, 1086, 1573, 2199 |
| NspV | TTCGAA | 2 cut(s) | 259, 2690 |
| PaeI | GCATGC | 5 cut(s) | 404, 408, 412, 531, 1573 |
| PceI | AGGCCT | 1 cut(s) | 2865 |
| PciI | ACATGT | 2 cut(s) | 1082, 2195 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 2703 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1328 |
| PfeI | GAWTC | 6 cut(s) | 1550, 1991, 2437, 2692, 2983, 3153 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1882 |
| PkrI | GCNGC | 2 cut(s) | 2059, 2611 |
| PleI | GAGTC | 6 cut(s) | 814, 1458, 1574, 1651, 1789, 3094 |
| PpsI | GAGTC | 6 cut(s) | 814, 1458, 1574, 1651, 1789, 3094 |
| PscI | ACATGT | 2 cut(s) | 1082, 2195 |
| PshBI | ATTAAT | 3 cut(s) | 233, 462, 2372 |
| PsiI | TTATAA | 1 cut(s) | 2274 |
| Psp124BI | GAGCTC | 1 cut(s) | 1840 |
| Psp6I | CCWGG | 2 cut(s) | 672, 1743 |
| PspFI | CCCAGC | 1 cut(s) | 1907 |
| PspGI | CCWGG | 2 cut(s) | 672, 1743 |
| PspN4I | GGNNCC | 3 cut(s) | 876, 1588, 2644 |
| PspPI | GGNCC | 1 cut(s) | 1901 |
| PsuI | RGATCY | 2 cut(s) | 2210, 2924 |
| PvuII | CAGCTG | 1 cut(s) | 1907 |
| RsaI | GTAC | 6 cut(s) | 1460, 1513, 2654, 2964, 2998, 3118 |
| RsaNI | GTAC | 6 cut(s) | 1459, 1512, 2653, 2963, 2997, 3117 |
| RseI | CAYNNNNRTG | 5 cut(s) | 1439, 1560, 1824, 2468, 2640 |
| SacI | GAGCTC | 1 cut(s) | 1840 |
| SatI | GCNGC | 2 cut(s) | 2058, 2610 |
| Sau3AI | GATC | 9 cut(s) | 24, 478, 561, 765, 1033, 1696, 2210, 2331, 2924 |
| Sau96I | GGNCC | 1 cut(s) | 1901 |
| ScaI | AGTACT | 1 cut(s) | 2654 |
| SchI | GAGTC | 6 cut(s) | 814, 1459, 1574, 1652, 1789, 3095 |
| ScrFI | CCNGG | 3 cut(s) | 674, 1745, 3054 |
| SduI | GDGCHC | 5 cut(s) | 1043, 1147, 1291, 1530, 1840 |
| SfaNI | GCATC | 4 cut(s) | 731, 732, 2317, 2761 |
| SfcI | CTRYAG | 3 cut(s) | 919, 1319, 2656 |
| SfuI | TTCGAA | 2 cut(s) | 259, 2690 |
| SinI | GGWCC | 1 cut(s) | 1901 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 1439, 1560, 1824, 2468, 2640 |
| SmlI | CTYRAG | 2 cut(s) | 1531, 1537 |
| SmoI | CTYRAG | 2 cut(s) | 1531, 1537 |
| SpeI | ACTAGT | 1 cut(s) | 1508 |
| SphI | GCATGC | 5 cut(s) | 404, 408, 412, 531, 1573 |
| SseBI | AGGCCT | 1 cut(s) | 2865 |
| SsiI | CCGC | 2 cut(s) | 204, 381 |
| SspI | AATATT | 3 cut(s) | 231, 460, 1375 |
| SstI | GAGCTC | 1 cut(s) | 1840 |
| StuI | AGGCCT | 1 cut(s) | 2865 |
| StyD4I | CCNGG | 3 cut(s) | 672, 1743, 3052 |
| StyI | CCWWGG | 6 cut(s) | 1138, 1668, 1810, 1830, 2558, 2966 |
| TaaI | ACNGT | 4 cut(s) | 1516, 2660, 2953, 2962 |
| TaiI | ACGT | 1 cut(s) | 374 |
| TatI | WGTACW | 2 cut(s) | 1511, 2652 |
| TfiI | GAWTC | 6 cut(s) | 1550, 1991, 2437, 2692, 2983, 3153 |
| TscAI | CASTG | 2 cut(s) | 780, 1858 |
| TseFI | GTSAC | 3 cut(s) | 860, 1201, 1792 |
| TseI | GCWGC | 2 cut(s) | 2057, 2609 |
| Tsp45I | GTSAC | 3 cut(s) | 860, 1201, 1792 |
| TspGWI | ACGGA | 5 cut(s) | 1026, 2120, 2203, 2984, 3032 |
| TspRI | CASTG | 2 cut(s) | 780, 1858 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1882 |
| VneI | GTGCAC | 1 cut(s) | 1039 |
| VpaK11BI | GGWCC | 1 cut(s) | 1901 |
| VspI | ATTAAT | 3 cut(s) | 233, 462, 2372 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 2179, 2454 |
| XapI | RAATTY | 9 cut(s) | 139, 261, 628, 1212, 2188, 2405, 2708, 2977, 3104 |
| XbaI | TCTAGA | 1 cut(s) | 1483 |
| XceI | RCATGY | 7 cut(s) | 404, 408, 412, 531, 1086, 1573, 2199 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 3134 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1328 |
| ZrmI | AGTACT | 1 cut(s) | 2654 |
| Zsp2I | ATGCAT | 3 cut(s) | 406, 410, 529 |