MD05G1256600.v1.1

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0017159
Physical Location & Seq
Forward (+)
38763669 .. 38765135
1467 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1256600.v1.1.491

Sequence Viewer

Length: 1467 bp
ATGGAAACACCCTTCACCAATACCACCCTCCTTTTCCTTCTAACCATAGCAACATTCTTGAATTTCACCGAAACCCTAGCCAGCCATCGCTCCAGTTCTACTTCCCTGGTGCTTGGACTAACACATTCTCGGGTTTCCCTCCCAAGTCCGAAAGCATCTTCTTACTCGAAAAAGATGCCATCACAAGTGTCAGACATGATCGAGCCACTGAGGGAAGTGAGAGATGGGTATCTGATACCTCTTAGTTTAGGGACTCCCCCACAAGTCATCGAAGTATATATGGATACTGGGAGTGACCTAACTTGGGTTCCTTGTGGCAATCTTTCTTTTGTTTGCATGGATTGTGATGACTATAGGAACAACAAACTAATGCCCATTTTCTCTCCTTCAGCTTCTTCTTCATCTCTTAGAGACCTTTGTAGTAGCTCATTTTGCATTGACATCCATAGCTCAGATAACTCCATCGATCCGTGCACCATAGTCGGATGTTCACTTGCTACCCTTCTTAAAGGCACATGTCCTAGACCATGCCCTTCATTTGCTTACACTTATGGCGCAGGGGGGGTTGTCCAAGGGGCACTTAGTAGAGACACATTAAGGGTTCATGGAATTAGTAGCACTCCTAATAATGTCGTCACTAGGGAAATTCCAAAGTTTTGTTTTGGGTGCATTTGGGCTAGTTATAGAGAGCCTATTGGGATTGCAGGGTTTGGTAGGGGAGCACTTTCCCTCCCATTCCAATTAGGGTTTCTACAAAAAGGCTTCTCTCATTGCTTCTTGGCTTTCAAGTATGCAAACAACCCTAATATTTCAAGTCCTCTAGTCATAGGAGATGTTGCTATTTCTTCAAAAGAAAACTTGCAATTCACTCCAATGTTGAAGAGTCCCATGTACCCTAGCAACTACTACATTGGTCTAGAGGCCATCACAATAGGTAATACTAGTACAGTTACACAAGTGCCCTCAAGCTTGAGAGAGTTTGATACACAAGGCAATGGGGGCATGCTCATTGACTCAGGAACCACTTACACTCATTTGCCTGAGCCATTTTACTCCAACCTTCTCTCCGTTATCGAGTCGGTTATTTGGTATCCTAGAGCCAAGGAAATGGAGACAAGGACCTCTTTTGATCTTTGTTATTTAGTCCCACACACAATGAATAATACTCTCATAGATCAGGATGACCTACTCCTTCCTCCAATCACTTTCCATTTCTTGAATAATGTGAATCTTGCTTTGCCCCAAGGAAATCACTTCTATGCCATGGGAGCTCCCGTAAACTCCAGTGTGGTGAAATGCTTGTTGTTCCAAGTAATGGATGATGGAGATTATGGGCCAGCTGGGGTGTTTGGGAGCTTCCAACAACAAAATGTGGAGGTTGTGTATGACTTGGAGAAAGAGAGAATTGGGTTTCAGCCAATGGATTGTGCTTCTGCTGCAGCCTCTCAAGGACTTCACAAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N TRAM_LAG1_CLN8

Protein Analysis

489

Amino Acids

53.37

Weight (kDa)

5.47

Isoelectric Point (pI)

43.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 77 - 278 2.1e-27 Xylanase inhibitor N-terminal
Asp PF00026 77 - 136 1.5e-07 Eukaryotic aspartyl protease
TAXi_C PF14541 301 - 472 5.8e-27 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017159)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45120
fragaria_vesca FvH4_3g11920
malus_domestica MD05G1256600.v1.1 MD10G1237100.v1.1
prunus_persica Prupe.4G107200_v2.0.a1
pyrus_communis pycom05g23590
rosa_chinensis RchiOBHm_Chr5g0019451
rosa_laevigata RLG00000032444
rosa_roxburghii Rroxscaffold_1G00058610
rosa_rugosa Rorug05G0051000
rosa_samantha Rh5BG140300 Rh5CG152000 Rh5DG140800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1315
AclWI GGATC 1 cut(s) 461
AcsI RAATTY 2 cut(s) 61, 645
AcuI CTGAAG 1 cut(s) 372
AfaI GTAC 2 cut(s) 893, 946
AfiI CCNNNNNNNGG 2 cut(s) 304, 1315
AflIII ACRYGT 1 cut(s) 515
AgsI TTSAA 6 cut(s) 61, 787, 813, 849, 880, 1219
AhlI ACTAGT 1 cut(s) 941
AjnI CCWGG 1 cut(s) 105
AjuI GAANNNNNNNTTGG 2 cut(s) 732, 764
AluBI AGCT 7 cut(s) 392, 426, 450, 969, 1271, 1340, 1356
AluI AGCT 7 cut(s) 392, 426, 450, 969, 1271, 1340, 1356
Alw21I GWGCWC 3 cut(s) 476, 724, 1273
Alw26I GTCTC 3 cut(s) 405, 582, 1106
Alw44I GTGCAC 1 cut(s) 472
AlwI GGATC 1 cut(s) 461
Ama87I CYCGRG 1 cut(s) 129
AoxI GGCC 2 cut(s) 921, 1334
ApaLI GTGCAC 1 cut(s) 472
ApeKI GCWGC 2 cut(s) 1436, 1439
ApoI RAATTY 2 cut(s) 61, 645
AspLEI GCGC 1 cut(s) 557
AspS9I GGNCC 2 cut(s) 1119, 1334
AsuHPI GGTGA 3 cut(s) 7, 58, 1303
AvaI CYCGRG 1 cut(s) 129
AvaII GGWCC 1 cut(s) 1119
BaeGI GKGCMC 3 cut(s) 476, 580, 963
BanII GRGCYC 1 cut(s) 1273
Bbv12I GWGCWC 3 cut(s) 476, 724, 1273
BbvI GCAGC 2 cut(s) 1423, 1451
BccI CCATC 6 cut(s) 93, 187, 218, 470, 932, 1316
BcgI CGANNNNNNTGC 4 cut(s) 157, 191, 463, 497
BciT130I CCWGG 1 cut(s) 107
BciVI GTATCC 2 cut(s) 277, 1101
BcoDI GTCTC 3 cut(s) 405, 582, 1106
BcuI ACTAGT 1 cut(s) 941
BfaI CTAG 9 cut(s) 77, 522, 639, 678, 821, 897, 917, 942, 1095
BfmI CTRYAG 2 cut(s) 352, 1437
BfuI GTATCC 2 cut(s) 277, 1101
BisI GCNGC 2 cut(s) 1437, 1440
BlsI GCNGC 2 cut(s) 1438, 1441
Bme1390I CCNGG 1 cut(s) 107
Bme18I GGWCC 1 cut(s) 1119
BmeT110I CYCGRG 1 cut(s) 129
BmgT120I GGNCC 2 cut(s) 1119, 1334
BmiI GGNNCC 2 cut(s) 309, 1021
BmrFI CCNGG 1 cut(s) 107
BmrI ACTGGG 1 cut(s) 297
BmsI GCATC 2 cut(s) 164, 165
BmuI ACTGGG 1 cut(s) 297
BpmI CTGGAG 2 cut(s) 76, 1267
Bpu10I CCTNAGC 1 cut(s) 1041
BpuEI CTTGAG 3 cut(s) 949, 991, 1431
Bsa29I ATCGAT 1 cut(s) 465
BsaBI GATNNNNATC 1 cut(s) 228
BsaI GGTCTC 1 cut(s) 405
BsaJI CCNNGG 5 cut(s) 105, 571, 1101, 1243, 1263
BsaXI ACNNNNNCTCC 8 cut(s) 714, 744, 1049, 1079, 1260, 1290, 1367, 1397
Bsc4I CCNNNNNNNGG 2 cut(s) 304, 1315
Bse1I ACTGG 3 cut(s) 93, 292, 1284
Bse3DI GCAATG 2 cut(s) 769, 1000
Bse8I GATNNNNATC 1 cut(s) 228
BseBI CCWGG 1 cut(s) 107
BseCI ATCGAT 1 cut(s) 465
BseDI CCNNGG 5 cut(s) 105, 571, 1101, 1243, 1263
BseGI GGATG 4 cut(s) 441, 491, 1186, 1324
BseJI GATNNNNATC 1 cut(s) 228
BseLI CCNNNNNNNGG 2 cut(s) 304, 1315
BseMI GCAATG 2 cut(s) 769, 1000
BseMII CTCAG 4 cut(s) 200, 465, 1029, 1032
BseNI ACTGG 3 cut(s) 93, 292, 1284
BseSI GKGCMC 3 cut(s) 476, 580, 963
BseXI GCAGC 2 cut(s) 1423, 1451
BseYI CCCAGC 1 cut(s) 1340
BshFI GGCC 2 cut(s) 923, 1336
BshVI ATCGAT 1 cut(s) 465
BsiHKAI GWGCWC 3 cut(s) 476, 724, 1273
BsiHKCI CYCGRG 1 cut(s) 129
BslFI GGGAC 3 cut(s) 265, 870, 1130
BslI CCNNNNNNNGG 2 cut(s) 304, 1315
BsmAI GTCTC 3 cut(s) 405, 582, 1106
BsmFI GGGAC 3 cut(s) 265, 870, 1130
BsnI GGCC 2 cut(s) 923, 1336
Bso31I GGTCTC 1 cut(s) 405
BsoBI CYCGRG 1 cut(s) 129
Bsp1286I GDGCHC 5 cut(s) 476, 580, 724, 963, 1273
Bsp143I GATC 4 cut(s) 198, 466, 1129, 1174
Bsp19I CCATGG 1 cut(s) 1263
BspANI GGCC 2 cut(s) 923, 1336
BspCNI CTCAG 4 cut(s) 201, 464, 1028, 1033
BspDI ATCGAT 1 cut(s) 465
BspLI GGNNCC 2 cut(s) 309, 1021
BspMAI CTGCAG 1 cut(s) 1441
BspPI GGATC 1 cut(s) 461
BspTNI GGTCTC 1 cut(s) 405
BsrDI GCAATG 2 cut(s) 769, 1000
BsrI ACTGG 3 cut(s) 93, 292, 1284
BssECI CCNNGG 5 cut(s) 105, 571, 1101, 1243, 1263
BssMI GATC 4 cut(s) 198, 466, 1129, 1174
BssT1I CCWWGG 4 cut(s) 571, 1101, 1243, 1263
Bst2UI CCWGG 1 cut(s) 107
Bst4CI ACNGT 1 cut(s) 949
Bst6I CTCTTC 1 cut(s) 875
BstC8I GCNNGC 3 cut(s) 82, 1004, 1338
BstDEI CTNAG 7 cut(s) 209, 242, 407, 451, 581, 1015, 1041
BstDSI CCRYGG 1 cut(s) 1263
BstF5I GGATG 4 cut(s) 441, 491, 1186, 1324
BstHHI GCGC 1 cut(s) 557
BstKTI GATC 4 cut(s) 201, 469, 1132, 1177
BstMAI GTCTC 3 cut(s) 405, 582, 1106
BstMBI GATC 4 cut(s) 198, 466, 1129, 1174
BstMWI GCNNNNNNNGC 4 cut(s) 432, 999, 1268, 1436
BstNI CCWGG 1 cut(s) 107
BstNSI RCATGY 2 cut(s) 519, 1006
BstSCI CCNGG 1 cut(s) 105
BstSFI CTRYAG 2 cut(s) 352, 1437
BstSLI GKGCMC 3 cut(s) 476, 580, 963
BstV1I GCAGC 2 cut(s) 1423, 1451
BstXI CCANNNNNNTGG 1 cut(s) 1108
Bsu15I ATCGAT 1 cut(s) 465
BsuI GTATCC 2 cut(s) 277, 1101
BsuRI GGCC 2 cut(s) 923, 1336
BsuTUI ATCGAT 1 cut(s) 465
BtgI CCRYGG 1 cut(s) 1263
BtgZI GCGATG 1 cut(s) 71
BtsCI GGATG 4 cut(s) 441, 491, 1186, 1324
BtsIMutI CAGTG 2 cut(s) 206, 1291
Cac8I GCNNGC 3 cut(s) 82, 1004, 1338
CfoI GCGC 1 cut(s) 557
Cfr13I GGNCC 2 cut(s) 1119, 1334
ClaI ATCGAT 1 cut(s) 465
Csp6I GTAC 2 cut(s) 892, 945
CviAII CATG 8 cut(s) 196, 337, 516, 528, 605, 889, 1003, 1264
CviQI GTAC 2 cut(s) 892, 945
DdeI CTNAG 7 cut(s) 209, 242, 407, 451, 581, 1015, 1041
DpnI GATC 4 cut(s) 200, 468, 1131, 1176
DpnII GATC 4 cut(s) 198, 466, 1129, 1174
Eam1104I CTCTTC 1 cut(s) 875
EarI CTCTTC 1 cut(s) 875
Ecl136II GAGCTC 1 cut(s) 1271
Eco130I CCWWGG 4 cut(s) 571, 1101, 1243, 1263
Eco24I GRGCYC 1 cut(s) 1273
Eco31I GGTCTC 1 cut(s) 405
Eco47I GGWCC 1 cut(s) 1119
Eco53kI GAGCTC 1 cut(s) 1271
Eco57I CTGAAG 1 cut(s) 372
Eco88I CYCGRG 1 cut(s) 129
EcoICRI GAGCTC 1 cut(s) 1271
EcoO109I RGGNCCY 1 cut(s) 1119
EcoRII CCWGG 1 cut(s) 105
EcoT14I CCWWGG 4 cut(s) 571, 1101, 1243, 1263
EcoT38I GRGCYC 1 cut(s) 1273
ErhI CCWWGG 4 cut(s) 571, 1101, 1243, 1263
FaeI CATG 8 cut(s) 199, 340, 519, 531, 608, 892, 1006, 1267
FalI AAGNNNNNCTT 4 cut(s) 564, 596, 1108, 1140
FaqI GGGAC 3 cut(s) 265, 870, 1130
FatI CATG 8 cut(s) 195, 336, 515, 527, 604, 888, 1002, 1263
Fnu4HI GCNGC 2 cut(s) 1437, 1440
FokI GGATG 4 cut(s) 428, 498, 1193, 1331
FriOI GRGCYC 1 cut(s) 1273
Fsp4HI GCNGC 2 cut(s) 1437, 1440
FspBI CTAG 9 cut(s) 77, 522, 639, 678, 821, 897, 917, 942, 1095
GlaI GCGC 1 cut(s) 556
GluI GCNGC 2 cut(s) 1437, 1440
GsaI CCCAGC 1 cut(s) 1344
GsuI CTGGAG 2 cut(s) 76, 1267
HaeIII GGCC 2 cut(s) 923, 1336
HhaI GCGC 1 cut(s) 557
Hin1II CATG 8 cut(s) 199, 340, 519, 531, 608, 892, 1006, 1267
Hin6I GCGC 1 cut(s) 555
HinP1I GCGC 1 cut(s) 555
HindIII AAGCTT 1 cut(s) 967
HinfI GANTC 5 cut(s) 253, 883, 1013, 1076, 1228
HphI GGTGA 3 cut(s) 7, 58, 1303
Hpy166II GTNNAC 3 cut(s) 474, 491, 1279
Hpy188I TCNGA 5 cut(s) 150, 193, 234, 454, 485
Hpy188III TCNNGA 5 cut(s) 58, 917, 1017, 1178, 1216
Hpy8I GTNNAC 3 cut(s) 474, 491, 1279
HpyAV CCTTC 7 cut(s) 22, 47, 396, 512, 543, 1070, 1202
HpyCH4III ACNGT 1 cut(s) 949
HpyCH4V TGCA 8 cut(s) 336, 435, 474, 669, 704, 794, 862, 1439
HpyF10VI GCNNNNNNNGC 4 cut(s) 432, 999, 1268, 1436
HpyF3I CTNAG 7 cut(s) 209, 242, 407, 451, 581, 1015, 1041
Hsp92II CATG 8 cut(s) 199, 340, 519, 531, 608, 892, 1006, 1267
HspAI GCGC 1 cut(s) 555
Kzo9I GATC 4 cut(s) 198, 466, 1129, 1174
LmnI GCTCC 5 cut(s) 95, 719, 1268, 1276, 1353
Lsp1109I GCAGC 2 cut(s) 1423, 1451
LweI GCATC 2 cut(s) 164, 165
MaeI CTAG 9 cut(s) 77, 522, 639, 678, 821, 897, 917, 942, 1095
MaeIII GTNAC 3 cut(s) 293, 634, 949
MalI GATC 4 cut(s) 200, 468, 1131, 1176
MboI GATC 4 cut(s) 198, 466, 1129, 1174
MboII GAAGA 5 cut(s) 150, 387, 390, 837, 892
MhlI GDGCHC 5 cut(s) 476, 580, 724, 963, 1273
MluCI AATT 6 cut(s) 61, 609, 645, 740, 863, 1404
MlyI GAGTC 4 cut(s) 247, 892, 1007, 1085
MmeI TCCRAC 3 cut(s) 463, 1080, 1384
MseI TTAA 2 cut(s) 507, 596
MslI CAYNNNNRTG 2 cut(s) 872, 1257
MspA1I CMGCKG 1 cut(s) 1340
MspR9I CCNGG 1 cut(s) 107
MvaI CCWGG 1 cut(s) 107
MwoI GCNNNNNNNGC 4 cut(s) 432, 999, 1268, 1436
NcoI CCATGG 1 cut(s) 1263
NdeII GATC 4 cut(s) 198, 466, 1129, 1174
NlaIII CATG 8 cut(s) 199, 340, 519, 531, 608, 892, 1006, 1267
NlaIV GGNNCC 2 cut(s) 309, 1021
NmuCI GTSAC 2 cut(s) 293, 634
NspI RCATGY 2 cut(s) 519, 1006
PaeI GCATGC 1 cut(s) 1006
PciI ACATGT 1 cut(s) 515
PfeI GAWTC 1 cut(s) 1228
PflMI CCANNNNNTGG 1 cut(s) 1315
PkrI GCNGC 2 cut(s) 1438, 1441
PleI GAGTC 4 cut(s) 247, 891, 1007, 1084
PpsI GAGTC 4 cut(s) 247, 891, 1007, 1084
PpuMI RGGWCCY 1 cut(s) 1119
PscI ACATGT 1 cut(s) 515
Psp124BI GAGCTC 1 cut(s) 1273
Psp5II RGGWCCY 1 cut(s) 1119
Psp6I CCWGG 1 cut(s) 105
PspFI CCCAGC 1 cut(s) 1340
PspGI CCWGG 1 cut(s) 105
PspN4I GGNNCC 2 cut(s) 309, 1021
PspPI GGNCC 2 cut(s) 1119, 1334
PspPPI RGGWCCY 1 cut(s) 1119
PstI CTGCAG 1 cut(s) 1441
PvuII CAGCTG 1 cut(s) 1340
RsaI GTAC 2 cut(s) 893, 946
RsaNI GTAC 2 cut(s) 892, 945
RseI CAYNNNNRTG 2 cut(s) 872, 1257
SacI GAGCTC 1 cut(s) 1273
SaqAI TTAA 2 cut(s) 507, 596
SatI GCNGC 2 cut(s) 1437, 1440
Sau3AI GATC 4 cut(s) 198, 466, 1129, 1174
Sau96I GGNCC 2 cut(s) 1119, 1334
SchI GAGTC 4 cut(s) 247, 892, 1007, 1085
ScrFI CCNGG 1 cut(s) 107
SduI GDGCHC 5 cut(s) 476, 580, 724, 963, 1273
SfaNI GCATC 2 cut(s) 164, 165
SfcI CTRYAG 2 cut(s) 352, 1437
SinI GGWCC 1 cut(s) 1119
SmiMI CAYNNNNRTG 2 cut(s) 872, 1257
SmlI CTYRAG 3 cut(s) 964, 970, 1446
SmoI CTYRAG 3 cut(s) 964, 970, 1446
SpeI ACTAGT 1 cut(s) 941
SphI GCATGC 1 cut(s) 1006
Sse9I AATT 6 cut(s) 61, 609, 645, 740, 863, 1404
SspI AATATT 1 cut(s) 808
SspMI CTAG 9 cut(s) 77, 522, 639, 678, 821, 897, 917, 942, 1095
SstI GAGCTC 1 cut(s) 1273
StyD4I CCNGG 1 cut(s) 105
StyI CCWWGG 4 cut(s) 571, 1101, 1243, 1263
TaaI ACNGT 1 cut(s) 949
TaqI TCGA 5 cut(s) 167, 201, 270, 465, 1074
TasI AATT 6 cut(s) 61, 609, 645, 740, 863, 1404
TatI WGTACW 1 cut(s) 944
TfiI GAWTC 1 cut(s) 1228
Tru1I TTAA 2 cut(s) 507, 596
Tru9I TTAA 2 cut(s) 507, 596
TscAI CASTG 2 cut(s) 213, 1291
TseFI GTSAC 2 cut(s) 293, 634
TseI GCWGC 2 cut(s) 1436, 1439
Tsp45I GTSAC 2 cut(s) 293, 634
TspDTI ATGAA 4 cut(s) 390, 525, 593, 1172
TspGWI ACGGA 2 cut(s) 459, 1057
TspRI CASTG 2 cut(s) 213, 1291
Van91I CCANNNNNTGG 1 cut(s) 1315
VneI GTGCAC 1 cut(s) 472
VpaK11BI GGWCC 1 cut(s) 1119
XapI RAATTY 2 cut(s) 61, 645
XbaI TCTAGA 1 cut(s) 916
XceI RCATGY 2 cut(s) 519, 1006
XspI CTAG 9 cut(s) 77, 522, 639, 678, 821, 897, 917, 942, 1095
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.