Rh5CG152000

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0017159
Physical Location & Seq
Reverse (-)
14118276 .. 14119935
1660 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG152000.1

Sequence Viewer

Length: 1449 bp
ATGGCGACACCCTTCACCAATAACCCCATCCTTATCCTTCTACTCATTGCATTCCTGAGTTTCAACCAAACCCTAGCCAGCCAGAGGTCCTGTACTACATCCCTAGTACTTGGTCTGAGGCATGCTTGGAGTTCCATTCGGAGTCCAACATCCTCCGCTAACTCAAAAAAGATACCATCACAAGTCTTAGACTTGATGGAGCCACTGAGGGAAGTCAGAGATGGGTATCTGATATCTCTCAATTTGGGGACACCCCCACAAGTCATTCAAGTTTATATGGATACTGGTAGTGACCTTACTTGGGTTCCTTGTGGAAATCTCTCTTTTAGTTGCATGGATTGTGATGACTATAGAAACACTAAACTAAACCCTACTTTCTCTCCTTCAGCTTCTTCTTCATCACTTAGAGACCTATGTGGTAGCCCCTTTTGCAAAGATATCCATAGCTCCGATAACGCTCTCGATCCATGCACCATGGCCGGTTGTTCACTCACTACCCTTATTAGGGGAACATGTCCTAGACCATGCCCTTCATTTGCTTATACCTATGGTGCGGGTGGGGTTGTCGTAGGGACCCTTGGTAGAGATACACTAAGGGTTCATGGATTTAGTAGTGGTAACCCTAGCAATGTCACTAGTGAAATTCCCAGTTTCTGTTTTGGGTGCATTGGGTCTACTTTTAGAGAGCCAATTGGTATTGTAGGGTTTGGTAGGGGAACGCTTTCCCTCCCATCGCAACTAGGGTTCCTACAAAAGGGCTTCTCTCACTGTTTCTTGGCCTTCAAGTATGTAAACAATCCCAACATTTCAAGCCCTCTAGTTATAGGAGATGTTGCTATTTCTTCTAAAGAAAATATGCAATTCACTCCAATGTTGAAGAGTCCCGTTTACCCTAATTACTACTATATTGGGATGGAAGCTATCACGATAGGTAATAATAGTATTACACAAGTGCCCTTAAGCTTGAGAGAGTTTGATTCACAAGGTAATGGGGGCATGTTGATTGACTCAGGAACCACTTATACTCATCTTCCTGAGCCATTTTACTCGGACGTTCTTTCAGTGCTCCAATCAGTGATAACATATCCTAGAGCCAAGGAAATGGAGATGAAGACTTCTTTTGATCTTTGTTATAAAGTTCCATACACAACCAATGCTTCCTCAGATGAGCTCTTTCCTTCAATCACTTTCCATTTCTTGAACAATGTAAGCCTTGGATTGCCTCAAGGCAATCACTTCTATGCTATGGGACCTCCAATCAACTCCACCGTGGTGAAATGCTTGTTGTTCCAAACCATGGATGATGGCGATTATGGGCCGGCCGGAGTGTTTGGGAGCTTCCAGCAACAAAATGTGGAGGTCGTGTATGACTTGGAGAAGGAGAGAATTGGATTTCAAACAATGGACTGTGCTTCAGCTGCAGCCTCTCAAGGCCTTCACAAAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N TRAM_LAG1_CLN8

Protein Analysis

482

Amino Acids

52.45

Weight (kDa)

5.76

Isoelectric Point (pI)

43.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 76 - 277 4.3e-29 Xylanase inhibitor N-terminal
Asp PF00026 76 - 134 9.8e-08 Eukaryotic aspartyl protease
TAXi_C PF14541 301 - 466 1.8e-29 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017159)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45120
fragaria_vesca FvH4_3g11920
malus_domestica MD05G1256600.v1.1 MD10G1237100.v1.1
prunus_persica Prupe.4G107200_v2.0.a1
pyrus_communis pycom05g23590
rosa_chinensis RchiOBHm_Chr5g0019451
rosa_laevigata RLG00000032444
rosa_roxburghii Rroxscaffold_1G00058610
rosa_rugosa Rorug05G0051000
rosa_samantha Rh5BG140300 Rh5CG152000 Rh5DG140800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1134
AccB7I CCANNNNNTGG 1 cut(s) 1297
AccI GTMKAC 1 cut(s) 674
AciI CCGC 2 cut(s) 156, 554
AclWI GGATC 1 cut(s) 458
AcoI YGGCCR 2 cut(s) 477, 1320
AcsI RAATTY 1 cut(s) 642
AcuI CTGAAG 2 cut(s) 369, 1398
AfaI GTAC 2 cut(s) 94, 108
AfiI CCNNNNNNNGG 6 cut(s) 84, 301, 504, 505, 754, 1297
AflII CTTAAG 1 cut(s) 958
AflIII ACRYGT 1 cut(s) 512
AgsI TTSAA 8 cut(s) 64, 269, 784, 810, 877, 1182, 1201, 1397
AhlI ACTAGT 1 cut(s) 635
AleI CACNNNNGTG 1 cut(s) 1271
AluBI AGCT 7 cut(s) 389, 447, 920, 963, 1171, 1338, 1418
AluI AGCT 7 cut(s) 389, 447, 920, 963, 1171, 1338, 1418
Alw21I GWGCWC 2 cut(s) 1068, 1173
Alw26I GTCTC 1 cut(s) 402
AlwI GGATC 1 cut(s) 458
AlwNI CAGNNNCTG 1 cut(s) 654
AoxI GGCC 5 cut(s) 477, 777, 1316, 1320, 1432
ApeKI GCWGC 2 cut(s) 1418, 1421
ApoI RAATTY 1 cut(s) 642
Asp700I GAANNNNTTC 1 cut(s) 721
AspS9I GGNCC 4 cut(s) 87, 573, 1250, 1316
AsuHPI GGTGA 2 cut(s) 7, 1285
AvaII GGWCC 3 cut(s) 87, 573, 1250
BaeGI GKGCMC 1 cut(s) 957
BanII GRGCYC 1 cut(s) 1173
BbsI GAAGAC 1 cut(s) 1118
Bbv12I GWGCWC 2 cut(s) 1068, 1173
BbvI GCAGC 2 cut(s) 1405, 1433
BccI CCATC 7 cut(s) 35, 184, 190, 215, 739, 907, 1298
BciVI GTATCC 1 cut(s) 274
BcoDI GTCTC 1 cut(s) 402
BcuI ACTAGT 1 cut(s) 635
BfaI CTAG 8 cut(s) 74, 104, 519, 624, 636, 740, 818, 1089
BfmI CTRYAG 2 cut(s) 349, 1419
BfrI CTTAAG 1 cut(s) 958
BfuI GTATCC 1 cut(s) 274
BisI GCNGC 2 cut(s) 1419, 1422
BlsI GCNGC 2 cut(s) 1420, 1423
BmcAI AGTACT 1 cut(s) 108
Bme18I GGWCC 3 cut(s) 87, 573, 1250
BmgT120I GGNCC 4 cut(s) 87, 573, 1250, 1316
BmiI GGNNCC 7 cut(s) 201, 306, 574, 575, 746, 1015, 1251
BmrI ACTGGG 1 cut(s) 642
BmuI ACTGGG 1 cut(s) 642
BpiI GAAGAC 1 cut(s) 1118
Bpu10I CCTNAGC 1 cut(s) 1035
BpuEI CTTGAG 3 cut(s) 985, 1209, 1413
BsaBI GATNNNNATC 1 cut(s) 225
BsaI GGTCTC 1 cut(s) 402
BsaJI CCNNGG 6 cut(s) 474, 577, 1095, 1213, 1269, 1296
BsaXI ACNNNNNCTCC 4 cut(s) 364, 394, 1349, 1379
Bsc4I CCNNNNNNNGG 6 cut(s) 84, 301, 504, 505, 754, 1297
Bse118I RCCGGY 2 cut(s) 479, 1318
Bse1I ACTGG 2 cut(s) 289, 648
Bse3DI GCAATG 2 cut(s) 45, 634
Bse8I GATNNNNATC 1 cut(s) 225
BseDI CCNNGG 6 cut(s) 474, 577, 1095, 1213, 1269, 1296
BseGI GGATG 5 cut(s) 27, 98, 149, 918, 1306
BseJI GATNNNNATC 1 cut(s) 225
BseLI CCNNNNNNNGG 6 cut(s) 84, 301, 504, 505, 754, 1297
BseMI GCAATG 2 cut(s) 45, 634
BseMII CTCAG 6 cut(s) 47, 107, 197, 1023, 1026, 1176
BseNI ACTGG 2 cut(s) 289, 648
BseSI GKGCMC 1 cut(s) 957
BseX3I CGGCCG 1 cut(s) 1320
BseXI GCAGC 2 cut(s) 1405, 1433
Bsh1285I CGRYCG 1 cut(s) 1323
BshFI GGCC 5 cut(s) 479, 779, 1318, 1322, 1434
BsiEI CGRYCG 1 cut(s) 1323
BsiHKAI GWGCWC 2 cut(s) 1068, 1173
BsiSI CCGG 3 cut(s) 480, 1319, 1323
BslFI GGGAC 4 cut(s) 262, 586, 867, 1263
BslI CCNNNNNNNGG 6 cut(s) 84, 301, 504, 505, 754, 1297
BsmAI GTCTC 1 cut(s) 402
BsmFI GGGAC 4 cut(s) 262, 586, 867, 1263
BsmI GAATGC 1 cut(s) 50
BsnI GGCC 5 cut(s) 479, 779, 1318, 1322, 1434
Bso31I GGTCTC 1 cut(s) 402
Bsp1286I GDGCHC 3 cut(s) 957, 1068, 1173
Bsp143I GATC 2 cut(s) 463, 1123
Bsp19I CCATGG 2 cut(s) 474, 1296
BspACI CCGC 2 cut(s) 156, 554
BspANI GGCC 5 cut(s) 479, 779, 1318, 1322, 1434
BspCNI CTCAG 6 cut(s) 48, 108, 198, 1022, 1027, 1175
BspLI GGNNCC 7 cut(s) 201, 306, 574, 575, 746, 1015, 1251
BspMAI CTGCAG 1 cut(s) 1423
BspPI GGATC 1 cut(s) 458
BspTI CTTAAG 1 cut(s) 958
BspTNI GGTCTC 1 cut(s) 402
BsrDI GCAATG 2 cut(s) 45, 634
BsrFI RCCGGY 2 cut(s) 479, 1318
BsrI ACTGG 2 cut(s) 289, 648
BssAI RCCGGY 2 cut(s) 479, 1318
BssECI CCNNGG 6 cut(s) 474, 577, 1095, 1213, 1269, 1296
BssMI GATC 2 cut(s) 463, 1123
BssT1I CCWWGG 5 cut(s) 474, 577, 1095, 1213, 1296
Bst4CI ACNGT 3 cut(s) 770, 1270, 1409
Bst6I CTCTTC 1 cut(s) 872
BstAFI CTTAAG 1 cut(s) 958
BstC8I GCNNGC 3 cut(s) 79, 123, 1320
BstDEI CTNAG 9 cut(s) 56, 116, 187, 206, 404, 593, 1009, 1035, 1162
BstDSI CCRYGG 3 cut(s) 474, 1269, 1296
BstEII GGTNACC 1 cut(s) 617
BstENI CCTNNNNNAGG 1 cut(s) 752
BstF5I GGATG 5 cut(s) 27, 98, 149, 918, 1306
BstKTI GATC 2 cut(s) 466, 1126
BstMAI GTCTC 1 cut(s) 402
BstMBI GATC 2 cut(s) 463, 1123
BstMCI CGRYCG 1 cut(s) 1323
BstMWI GCNNNNNNNGC 2 cut(s) 429, 1418
BstNSI RCATGY 3 cut(s) 125, 516, 1000
BstPI GGTNACC 1 cut(s) 617
BstSFI CTRYAG 2 cut(s) 349, 1419
BstSLI GKGCMC 1 cut(s) 957
BstV1I GCAGC 2 cut(s) 1405, 1433
BstV2I GAAGAC 1 cut(s) 1118
BstXI CCANNNNNNTGG 1 cut(s) 1102
BstZI CGGCCG 1 cut(s) 1320
BsuI GTATCC 1 cut(s) 274
BsuRI GGCC 5 cut(s) 479, 779, 1318, 1322, 1434
BtgI CCRYGG 3 cut(s) 474, 1269, 1296
BtgZI GCGATG 1 cut(s) 717
BtsCI GGATG 5 cut(s) 27, 98, 149, 918, 1306
BtsIMutI CAGTG 4 cut(s) 203, 766, 1068, 1080
Cac8I GCNNGC 3 cut(s) 79, 123, 1320
CaiI CAGNNNCTG 1 cut(s) 654
Cfr10I RCCGGY 2 cut(s) 479, 1318
Cfr13I GGNCC 4 cut(s) 87, 573, 1250, 1316
Csp6I GTAC 2 cut(s) 93, 107
CviAII CATG 9 cut(s) 122, 334, 468, 475, 513, 525, 602, 997, 1297
CviQI GTAC 2 cut(s) 93, 107
DdeI CTNAG 9 cut(s) 56, 116, 187, 206, 404, 593, 1009, 1035, 1162
DpnI GATC 2 cut(s) 465, 1125
DpnII GATC 2 cut(s) 463, 1123
EaeI YGGCCR 2 cut(s) 477, 1320
EagI CGGCCG 1 cut(s) 1320
Eam1104I CTCTTC 1 cut(s) 872
EarI CTCTTC 1 cut(s) 872
Ecl136II GAGCTC 1 cut(s) 1171
EclXI CGGCCG 1 cut(s) 1320
Eco130I CCWWGG 5 cut(s) 474, 577, 1095, 1213, 1296
Eco147I AGGCCT 1 cut(s) 1434
Eco24I GRGCYC 1 cut(s) 1173
Eco31I GGTCTC 1 cut(s) 402
Eco32I GATATC 2 cut(s) 234, 439
Eco47I GGWCC 3 cut(s) 87, 573, 1250
Eco52I CGGCCG 1 cut(s) 1320
Eco53kI GAGCTC 1 cut(s) 1171
Eco57I CTGAAG 2 cut(s) 369, 1398
Eco91I GGTNACC 1 cut(s) 617
EcoICRI GAGCTC 1 cut(s) 1171
EcoNI CCTNNNNNAGG 1 cut(s) 752
EcoO109I RGGNCCY 3 cut(s) 87, 573, 1250
EcoO65I GGTNACC 1 cut(s) 617
EcoRV GATATC 2 cut(s) 234, 439
EcoT14I CCWWGG 5 cut(s) 474, 577, 1095, 1213, 1296
EcoT38I GRGCYC 1 cut(s) 1173
ErhI CCWWGG 5 cut(s) 474, 577, 1095, 1213, 1296
FaeI CATG 9 cut(s) 125, 337, 471, 478, 516, 528, 605, 1000, 1300
FaqI GGGAC 4 cut(s) 262, 586, 867, 1263
FatI CATG 9 cut(s) 121, 333, 467, 474, 512, 524, 601, 996, 1296
FauI CCCGC 1 cut(s) 547
FblI GTMKAC 1 cut(s) 674
Fnu4HI GCNGC 2 cut(s) 1419, 1422
FokI GGATG 5 cut(s) 14, 85, 136, 925, 1313
FriOI GRGCYC 1 cut(s) 1173
FseI GGCCGGCC 1 cut(s) 1322
Fsp4HI GCNGC 2 cut(s) 1419, 1422
FspBI CTAG 8 cut(s) 74, 104, 519, 624, 636, 740, 818, 1089
GluI GCNGC 2 cut(s) 1419, 1422
HaeIII GGCC 5 cut(s) 479, 779, 1318, 1322, 1434
HapII CCGG 3 cut(s) 480, 1319, 1323
Hin1II CATG 9 cut(s) 125, 337, 471, 478, 516, 528, 605, 1000, 1300
HindIII AAGCTT 1 cut(s) 961
HinfI GANTC 4 cut(s) 142, 880, 977, 1007
HpaII CCGG 3 cut(s) 480, 1319, 1323
HphI GGTGA 2 cut(s) 7, 1285
Hpy166II GTNNAC 4 cut(s) 488, 675, 793, 889
Hpy188I TCNGA 7 cut(s) 117, 141, 218, 231, 451, 1051, 1165
Hpy188III TCNNGA 6 cut(s) 55, 461, 925, 1011, 1034, 1198
Hpy8I GTNNAC 4 cut(s) 488, 675, 793, 889
HpyAV CCTTC 8 cut(s) 22, 47, 393, 540, 790, 1188, 1372, 1445
HpyCH4III ACNGT 3 cut(s) 770, 1270, 1409
HpyCH4IV ACGT 1 cut(s) 1053
HpyCH4V TGCA 7 cut(s) 50, 333, 432, 471, 666, 859, 1421
HpyF10VI GCNNNNNNNGC 2 cut(s) 429, 1418
HpyF3I CTNAG 9 cut(s) 56, 116, 187, 206, 404, 593, 1009, 1035, 1162
HpySE526I ACGT 1 cut(s) 1053
Hsp92II CATG 9 cut(s) 125, 337, 471, 478, 516, 528, 605, 1000, 1300
KflI GGGWCCC 1 cut(s) 573
KroI GCCGGC 1 cut(s) 1318
KroNI GCCGGC 1 cut(s) 1320
Kzo9I GATC 2 cut(s) 463, 1123
LmnI GCTCC 4 cut(s) 199, 452, 1071, 1335
Lsp1109I GCAGC 2 cut(s) 1405, 1433
MaeI CTAG 8 cut(s) 74, 104, 519, 624, 636, 740, 818, 1089
MaeII ACGT 1 cut(s) 1053
MaeIII GTNAC 3 cut(s) 290, 617, 631
MalI GATC 2 cut(s) 465, 1125
MboI GATC 2 cut(s) 463, 1123
MboII GAAGA 6 cut(s) 384, 387, 834, 889, 1022, 1123
MfeI CAATTG 1 cut(s) 690
MhlI GDGCHC 3 cut(s) 957, 1068, 1173
MluCI AATT 6 cut(s) 241, 642, 690, 860, 895, 1386
MlyI GAGTC 3 cut(s) 151, 889, 1001
MmeI TCCRAC 1 cut(s) 170
MroNI GCCGGC 1 cut(s) 1318
MroXI GAANNNNTTC 1 cut(s) 721
MseI TTAA 1 cut(s) 959
MslI CAYNNNNRTG 3 cut(s) 869, 1239, 1271
MspA1I CMGCKG 1 cut(s) 1418
MspCI CTTAAG 1 cut(s) 958
MspI CCGG 3 cut(s) 480, 1319, 1323
MunI CAATTG 1 cut(s) 690
Mva1269I GAATGC 1 cut(s) 50
MwoI GCNNNNNNNGC 2 cut(s) 429, 1418
NaeI GCCGGC 1 cut(s) 1320
NcoI CCATGG 2 cut(s) 474, 1296
NdeII GATC 2 cut(s) 463, 1123
NgoMIV GCCGGC 1 cut(s) 1318
NlaIII CATG 9 cut(s) 125, 337, 471, 478, 516, 528, 605, 1000, 1300
NlaIV GGNNCC 7 cut(s) 201, 306, 574, 575, 746, 1015, 1251
NmuCI GTSAC 2 cut(s) 290, 631
NspI RCATGY 3 cut(s) 125, 516, 1000
OliI CACNNNNGTG 1 cut(s) 1271
PaeI GCATGC 1 cut(s) 125
PceI AGGCCT 1 cut(s) 1434
PciI ACATGT 1 cut(s) 512
PctI GAATGC 1 cut(s) 50
PdiI GCCGGC 1 cut(s) 1320
PdmI GAANNNNTTC 1 cut(s) 721
PfeI GAWTC 1 cut(s) 977
PflMI CCANNNNNTGG 1 cut(s) 1297
PkrI GCNGC 2 cut(s) 1420, 1423
PleI GAGTC 3 cut(s) 150, 888, 1001
PpsI GAGTC 3 cut(s) 150, 888, 1001
PpuMI RGGWCCY 3 cut(s) 87, 573, 1250
PscI ACATGT 1 cut(s) 512
PsiI TTATAA 1 cut(s) 1134
Psp124BI GAGCTC 1 cut(s) 1173
Psp5II RGGWCCY 3 cut(s) 87, 573, 1250
PspEI GGTNACC 1 cut(s) 617
PspN4I GGNNCC 7 cut(s) 201, 306, 574, 575, 746, 1015, 1251
PspPI GGNCC 4 cut(s) 87, 573, 1250, 1316
PspPPI RGGWCCY 3 cut(s) 87, 573, 1250
PsrI GAACNNNNNNTAC 2 cut(s) 1006, 1038
PstI CTGCAG 1 cut(s) 1423
PstNI CAGNNNCTG 1 cut(s) 654
PvuII CAGCTG 1 cut(s) 1418
RigI GGCCGGCC 1 cut(s) 1322
RsaI GTAC 2 cut(s) 94, 108
RsaNI GTAC 2 cut(s) 93, 107
RseI CAYNNNNRTG 3 cut(s) 869, 1239, 1271
SacI GAGCTC 1 cut(s) 1173
SaqAI TTAA 1 cut(s) 959
SatI GCNGC 2 cut(s) 1419, 1422
Sau3AI GATC 2 cut(s) 463, 1123
Sau96I GGNCC 4 cut(s) 87, 573, 1250, 1316
ScaI AGTACT 1 cut(s) 108
SchI GAGTC 3 cut(s) 151, 889, 1001
SduI GDGCHC 3 cut(s) 957, 1068, 1173
SfcI CTRYAG 2 cut(s) 349, 1419
SinI GGWCC 3 cut(s) 87, 573, 1250
SmiMI CAYNNNNRTG 3 cut(s) 869, 1239, 1271
SmlI CTYRAG 4 cut(s) 958, 964, 1224, 1428
SmoI CTYRAG 4 cut(s) 958, 964, 1224, 1428
SpeI ACTAGT 1 cut(s) 635
SphI GCATGC 1 cut(s) 125
Sse9I AATT 6 cut(s) 241, 642, 690, 860, 895, 1386
SseBI AGGCCT 1 cut(s) 1434
SsiI CCGC 2 cut(s) 156, 554
SspMI CTAG 8 cut(s) 74, 104, 519, 624, 636, 740, 818, 1089
SstI GAGCTC 1 cut(s) 1173
StuI AGGCCT 1 cut(s) 1434
StyI CCWWGG 5 cut(s) 474, 577, 1095, 1213, 1296
TaaI ACNGT 3 cut(s) 770, 1270, 1409
TaiI ACGT 1 cut(s) 1056
TaqI TCGA 1 cut(s) 462
TasI AATT 6 cut(s) 241, 642, 690, 860, 895, 1386
TatI WGTACW 2 cut(s) 92, 106
TfiI GAWTC 1 cut(s) 977
Tru1I TTAA 1 cut(s) 959
Tru9I TTAA 1 cut(s) 959
TscAI CASTG 4 cut(s) 210, 773, 1068, 1080
TseFI GTSAC 2 cut(s) 290, 631
TseI GCWGC 2 cut(s) 1418, 1421
Tsp45I GTSAC 2 cut(s) 290, 631
TspDTI ATGAA 4 cut(s) 387, 522, 590, 1124
TspRI CASTG 4 cut(s) 210, 773, 1068, 1080
Van91I CCANNNNNTGG 1 cut(s) 1297
Vha464I CTTAAG 1 cut(s) 958
VpaK11BI GGWCC 3 cut(s) 87, 573, 1250
XagI CCTNNNNNAGG 1 cut(s) 752
XapI RAATTY 1 cut(s) 642
XceI RCATGY 3 cut(s) 125, 516, 1000
XmiI GTMKAC 1 cut(s) 674
XmnI GAANNNNTTC 1 cut(s) 721
XspI CTAG 8 cut(s) 74, 104, 519, 624, 636, 740, 818, 1089
ZrmI AGTACT 1 cut(s) 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.