AT5G46350
WRKY Family

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012414
Physical Location & Seq
Reverse (-)
18801164 .. 18804056
2893 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G46350.1

Sequence Viewer

Length: 981 bp
ATGTCTCATGAAATCAAAGATCTTAACAACTATCACTACACTTCATCGTATAATCATTACAATATCAACAACCAAAATATGATTAATCTCCCTTACGTTTCTGGTCCATCTGCTTATAATGCAAACATGATCTCATCATCACAAGTAGGTTTTGATCTACCCTCGAAGAACTTGAGTCCTCAAGGAGCCTTCGAGTTGGGTTTCGAGCTTTCTCCATCTTCTTCTGACTTTTTTAATCCTTCCCTCGATCAAGAGAACGGTTTGTATAATGCTTATAATTATAATAGTAGTCAAAAGAGTCATGAAGTTGTCGGTGATGGTTGTGCAACCATTAAGAGTGAAGTTAGGGTTTCAGCATCTCCTTCTTCAAGTGAGGCCGATCATCATCCAGGAGAAGATTCCGGCAAGATCCGGAAGAAAAGAGAAGTTCGCGATGGAGGAGAAGATGATCAACGCTCTCAGAAAGTAGTTAAAACAAAGAAGAAAGAGGAGAAGAAAAAAGAGCCACGAGTCTCGTTCATGACTAAGACCGAAGTTGATCATCTCGAAGACGGCTATCGTTGGAGAAAGTATGGCCAAAAAGCAGTCAAAAACAGTCCTTATCCGAGGAGTTACTATAGATGCACGACTCAGAAGTGCAACGTGAAGAAGAGAGTGGAGAGATCTTACCAAGACCCAACGGTCGTCATCACAACCTACGAGAGTCAACACAACCATCCGATCCCGACCAATCGTCGGACAGCAATGTTCTCTGGAACCACCGCATCTGATTATAACCCATCATCGTCTCCAATATTCTCCGATCTCATCATCAATACTCCAAGAAGCTTCTCAAATGATGATCTCTTCCGTGTGCCATACGCTAGTGTGAACGTGAACCCTAGTTATCATCAACAGCAACATGGATTTCATCAACAGGAGAGTGAGTTCGAGCTCTTGAAGGAGATGTTTCCTTCGGTTTTCTTCAAACAAGAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002252 GO:0002376 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0006979 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009615 GO:0009617 GO:0009620 GO:0009636 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010035 GO:0010228 GO:0010453 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0033554 GO:0033993 GO:0034599 GO:0034614 GO:0035690 GO:0042221 GO:0042493 GO:0042542 GO:0042659 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0045595 GO:0045893 GO:0045935 GO:0046677 GO:0047484 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051607 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070301 GO:0070887 GO:0071236 GO:0080090 GO:0080134 GO:0097159 GO:0097237 GO:0097305 GO:0098542 GO:0140110 GO:1901000 GO:1901002 GO:1901363 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

326

Amino Acids

37.29

Weight (kDa)

7.73

Isoelectric Point (pI)

56.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 183 - 240 3.6e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012414)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29860 AT4G18170 AT5G46350
fragaria_vesca FvH4_3g06200
malus_domestica MD05G1290300.v1.1 MD10G1266400.v1.1
prunus_persica Prupe.4G075400_v2.0.a1 Prupe.4G075400_v2.0.a1
pyrus_communis pycom05g26710 pycom10g22220
rosa_chinensis RchiOBHm_Chr5g0013131
rosa_laevigata RLG00000031991
rosa_multiflora Rmu_sc0003892.1_g000004
rosa_roxburghii Rroxscaffold_1G00063450
rosa_samantha Rh5AG101600 Rh5BG098000 Rh5CG110100 Rh5DG097200
rosa_wichuraiana Rw5G008880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 117, 276, 282, 774
AasI GACNNNNNNGTC 1 cut(s) 680
AccII CGCG 1 cut(s) 432
AccIII TCCGGA 1 cut(s) 411
AciI CCGC 1 cut(s) 762
AclWI GGATC 2 cut(s) 403, 715
AcoI YGGCCR 1 cut(s) 574
AfiI CCNNNNNNNGG 1 cut(s) 735
AgsI TTSAA 3 cut(s) 369, 940, 967
AhdI GACNNNNNGTC 1 cut(s) 732
AjnI CCWGG 1 cut(s) 388
AloI GAACNNNNNNTCC 2 cut(s) 911, 943
AluBI AGCT 3 cut(s) 208, 828, 934
AluI AGCT 3 cut(s) 208, 828, 934
Alw21I GWGCWC 1 cut(s) 936
Alw26I GTCTC 3 cut(s) 9, 517, 792
AlwI GGATC 2 cut(s) 403, 715
Aor13HI TCCGGA 1 cut(s) 411
AoxI GGCC 2 cut(s) 375, 574
AseI ATTAAT 1 cut(s) 84
AspS9I GGNCC 1 cut(s) 104
AsuHPI GGTGA 1 cut(s) 326
AvaII GGWCC 1 cut(s) 104
BalI TGGCCA 1 cut(s) 576
BanII GRGCYC 1 cut(s) 936
BauI CACGAG 1 cut(s) 507
BbsI GAAGAC 1 cut(s) 555
Bbv12I GWGCWC 1 cut(s) 936
BccI CCATC 6 cut(s) 115, 223, 311, 428, 723, 787
BceAI ACGGC 1 cut(s) 568
BciT130I CCWGG 1 cut(s) 390
BclI TGATCA 2 cut(s) 448, 538
BcoDI GTCTC 3 cut(s) 9, 517, 792
BfaI CTAG 2 cut(s) 864, 882
BfmI CTRYAG 1 cut(s) 616
BglII AGATCT 2 cut(s) 19, 662
Bme1390I CCNGG 1 cut(s) 390
Bme18I GGWCC 1 cut(s) 104
BmeRI GACNNNNNGTC 1 cut(s) 732
BmgT120I GGNCC 1 cut(s) 104
BmiI GGNNCC 2 cut(s) 187, 757
BmrFI CCNGG 1 cut(s) 390
BmsI GCATC 3 cut(s) 365, 611, 773
BpiI GAAGAC 1 cut(s) 555
BpuEI CTTGAG 2 cut(s) 165, 193
BsaBI GATNNNNATC 1 cut(s) 384
BsaJI CCNNGG 1 cut(s) 605
BsaWI WCCGGW 1 cut(s) 411
BsaXI ACNNNNNCTCC 2 cut(s) 911, 941
Bsc4I CCNNNNNNNGG 1 cut(s) 735
Bse3DI GCAATG 1 cut(s) 750
Bse8I GATNNNNATC 1 cut(s) 384
BseAI TCCGGA 1 cut(s) 411
BseBI CCWGG 1 cut(s) 390
BseDI CCNNGG 1 cut(s) 605
BseGI GGATG 2 cut(s) 385, 715
BseJI GATNNNNATC 1 cut(s) 384
BseLI CCNNNNNNNGG 1 cut(s) 735
BseMI GCAATG 1 cut(s) 750
BseMII CTCAG 2 cut(s) 473, 644
BseRI GAGGAG 3 cut(s) 453, 503, 622
Bsh1236I CGCG 1 cut(s) 432
Bsh1285I CGRYCG 1 cut(s) 684
BshFI GGCC 2 cut(s) 377, 576
BsiEI CGRYCG 1 cut(s) 684
BsiHKAI GWGCWC 1 cut(s) 936
BsiSI CCGG 2 cut(s) 402, 412
BslI CCNNNNNNNGG 1 cut(s) 735
BsmAI GTCTC 3 cut(s) 9, 517, 792
BsmBI CGTCTC 1 cut(s) 792
BsnI GGCC 2 cut(s) 377, 576
Bsp1286I GDGCHC 1 cut(s) 936
Bsp13I TCCGGA 1 cut(s) 411
Bsp68I TCGCGA 1 cut(s) 432
BspACI CCGC 1 cut(s) 762
BspANI GGCC 2 cut(s) 377, 576
BspCNI CTCAG 2 cut(s) 472, 643
BspEI TCCGGA 1 cut(s) 411
BspFNI CGCG 1 cut(s) 432
BspHI TCATGA 3 cut(s) 7, 301, 519
BspLI GGNNCC 2 cut(s) 187, 757
BspPI GGATC 2 cut(s) 403, 715
BsrDI GCAATG 1 cut(s) 750
BssECI CCNNGG 1 cut(s) 605
BssSI CACGAG 1 cut(s) 507
Bst2BI CACGAG 1 cut(s) 507
Bst2UI CCWGG 1 cut(s) 390
Bst4CI ACNGT 3 cut(s) 260, 596, 682
Bst6I CTCTTC 2 cut(s) 644, 851
BstDEI CTNAG 3 cut(s) 459, 525, 630
BstF5I GGATG 2 cut(s) 385, 715
BstFNI CGCG 1 cut(s) 432
BstMAI GTCTC 3 cut(s) 9, 517, 792
BstMCI CGRYCG 1 cut(s) 684
BstMWI GCNNNNNNNGC 1 cut(s) 119
BstNI CCWGG 1 cut(s) 390
BstSCI CCNGG 1 cut(s) 388
BstSFI CTRYAG 1 cut(s) 616
BstUI CGCG 1 cut(s) 432
BstV2I GAAGAC 1 cut(s) 555
BstX2I RGATCY 3 cut(s) 19, 408, 662
BstYI RGATCY 3 cut(s) 19, 408, 662
BsuRI GGCC 2 cut(s) 377, 576
BtgZI GCGATG 1 cut(s) 447
BtsCI GGATG 2 cut(s) 385, 715
BtuMI TCGCGA 1 cut(s) 432
CciI TCATGA 3 cut(s) 7, 301, 519
Cfr13I GGNCC 1 cut(s) 104
CviAII CATG 5 cut(s) 8, 127, 302, 520, 902
CviJI RGCY 9 cut(s) 188, 208, 377, 505, 555, 576, 828, 934, 976
CviKI_1 RGCY 9 cut(s) 188, 208, 377, 505, 555, 576, 828, 934, 976
DdeI CTNAG 3 cut(s) 459, 525, 630
DrdI GACNNNNNNGTC 1 cut(s) 680
DriI GACNNNNNGTC 1 cut(s) 732
DseDI GACNNNNNNGTC 1 cut(s) 680
EaeI YGGCCR 1 cut(s) 574
Eam1104I CTCTTC 2 cut(s) 644, 851
Eam1105I GACNNNNNGTC 1 cut(s) 732
EarI CTCTTC 2 cut(s) 644, 851
Ecl136II GAGCTC 1 cut(s) 934
Eco24I GRGCYC 1 cut(s) 936
Eco47I GGWCC 1 cut(s) 104
Eco53kI GAGCTC 1 cut(s) 934
EcoICRI GAGCTC 1 cut(s) 934
EcoRII CCWGG 1 cut(s) 388
EcoT38I GRGCYC 1 cut(s) 936
Esp3I CGTCTC 1 cut(s) 792
FaeI CATG 5 cut(s) 11, 130, 305, 523, 905
FatI CATG 5 cut(s) 7, 126, 301, 519, 901
FbaI TGATCA 2 cut(s) 448, 538
FokI GGATG 2 cut(s) 372, 702
FriOI GRGCYC 1 cut(s) 936
FspBI CTAG 2 cut(s) 864, 882
HaeIII GGCC 2 cut(s) 377, 576
HapII CCGG 2 cut(s) 402, 412
Hin1II CATG 5 cut(s) 11, 130, 305, 523, 905
HincII GTYRAC 1 cut(s) 707
HindII GTYRAC 1 cut(s) 707
HindIII AAGCTT 1 cut(s) 826
HinfI GANTC 6 cut(s) 175, 298, 398, 510, 628, 703
HpaII CCGG 2 cut(s) 402, 412
HphI GGTGA 1 cut(s) 326
Hpy166II GTNNAC 3 cut(s) 707, 871, 877
Hpy188I TCNGA 8 cut(s) 226, 462, 606, 633, 720, 738, 769, 802
Hpy8I GTNNAC 3 cut(s) 707, 871, 877
Hpy99I CGWCG 1 cut(s) 738
HpyAV CCTTC 5 cut(s) 199, 249, 372, 934, 963
HpyCH4III ACNGT 3 cut(s) 260, 596, 682
HpyCH4IV ACGT 3 cut(s) 96, 642, 873
HpyCH4V TGCA 4 cut(s) 122, 326, 624, 639
HpyF10VI GCNNNNNNNGC 1 cut(s) 119
HpyF3I CTNAG 3 cut(s) 459, 525, 630
HpySE526I ACGT 3 cut(s) 96, 642, 873
Hsp92II CATG 5 cut(s) 11, 130, 305, 523, 905
Kpn2I TCCGGA 1 cut(s) 411
Ksp22I TGATCA 2 cut(s) 448, 538
LmnI GCTCC 1 cut(s) 185
LpnPI CCDG 7 cut(s) 87, 375, 402, 415, 425, 738, 902
LweI GCATC 3 cut(s) 365, 611, 773
MaeI CTAG 2 cut(s) 864, 882
MaeII ACGT 3 cut(s) 96, 642, 873
MaeIII GTNAC 1 cut(s) 611
MflI RGATCY 3 cut(s) 19, 408, 662
MhlI GDGCHC 1 cut(s) 936
MlsI TGGCCA 1 cut(s) 576
MluCI AATT 1 cut(s) 277
MluNI TGGCCA 1 cut(s) 576
MlyI GAGTC 5 cut(s) 184, 307, 519, 622, 712
MmeI TCCRAC 2 cut(s) 542, 716
MnlI CCTC 7 cut(s) 172, 189, 254, 367, 431, 481, 600
Mox20I TGGCCA 1 cut(s) 576
MroI TCCGGA 1 cut(s) 411
MscI TGGCCA 1 cut(s) 576
MseI TTAA 5 cut(s) 24, 84, 234, 333, 471
Msp20I TGGCCA 1 cut(s) 576
MspI CCGG 2 cut(s) 402, 412
MspR9I CCNGG 1 cut(s) 390
MvaI CCWGG 1 cut(s) 390
MvnI CGCG 1 cut(s) 432
MwoI GCNNNNNNNGC 1 cut(s) 119
NlaIII CATG 5 cut(s) 11, 130, 305, 523, 905
NlaIV GGNNCC 2 cut(s) 187, 757
NruI TCGCGA 1 cut(s) 432
PagI TCATGA 3 cut(s) 7, 301, 519
PfeI GAWTC 1 cut(s) 398
PfoI TCCNGGA 1 cut(s) 388
PleI GAGTC 5 cut(s) 183, 306, 518, 622, 711
PpsI GAGTC 5 cut(s) 183, 306, 518, 622, 711
PshBI ATTAAT 1 cut(s) 84
PsiI TTATAA 4 cut(s) 117, 276, 282, 774
Psp124BI GAGCTC 1 cut(s) 936
Psp6I CCWGG 1 cut(s) 388
PspGI CCWGG 1 cut(s) 388
PspN4I GGNNCC 2 cut(s) 187, 757
PspPI GGNCC 1 cut(s) 104
PsuI RGATCY 3 cut(s) 19, 408, 662
RruI TCGCGA 1 cut(s) 432
SacI GAGCTC 1 cut(s) 936
SaqAI TTAA 5 cut(s) 24, 84, 234, 333, 471
Sau96I GGNCC 1 cut(s) 104
SchI GAGTC 5 cut(s) 184, 307, 519, 622, 712
ScrFI CCNGG 1 cut(s) 390
SduI GDGCHC 1 cut(s) 936
SetI ASST 8 cut(s) 99, 151, 210, 645, 698, 830, 876, 936
SfaNI GCATC 3 cut(s) 365, 611, 773
SfcI CTRYAG 1 cut(s) 616
SinI GGWCC 1 cut(s) 104
SmlI CTYRAG 2 cut(s) 172, 180
SmoI CTYRAG 2 cut(s) 172, 180
Sse9I AATT 1 cut(s) 277
SsiI CCGC 1 cut(s) 762
SspI AATATT 1 cut(s) 795
SspMI CTAG 2 cut(s) 864, 882
SstI GAGCTC 1 cut(s) 936
StyD4I CCNGG 1 cut(s) 388
TaaI ACNGT 3 cut(s) 260, 596, 682
TaiI ACGT 3 cut(s) 99, 645, 876
TaqI TCGA 6 cut(s) 164, 192, 204, 246, 546, 930
TaqII GACCGA 1 cut(s) 545
TasI AATT 1 cut(s) 277
TfiI GAWTC 1 cut(s) 398
Tru1I TTAA 5 cut(s) 24, 84, 234, 333, 471
Tru9I TTAA 5 cut(s) 24, 84, 234, 333, 471
TspDTI ATGAA 5 cut(s) 24, 33, 318, 508, 899
TspGWI ACGGA 1 cut(s) 839
VpaK11BI GGWCC 1 cut(s) 104
VspI ATTAAT 1 cut(s) 84
XspI CTAG 2 cut(s) 864, 882
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.