RLG00000031991
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0012414
Physical Location & Seq
Forward (+)
10048915 .. 10050560
1646 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031991

Sequence Viewer

Length: 1065 bp
ATGTCTGATCATCATCATGAACCTAAAGACCTTTACTACCATGACCTATTTCAGTATGATGATCACGGACAACTTAATGGTGGAATGATCATGAACAACCCAAAAGTTTCTGGTTCATCATCCCCAGCTTACAATAATCTATTACAGGGGTTTGATGTTCCTTCATACATGAACACCAGCTTCACAGAGTACAACTCACTTGCAACGGCTTTCGGCTTATCGTCGTCACCCTCCGATCACGAAGTATTTTCCTCCATTGATGAAGGCAGCCATCAAAAGCCTGTTGATAATCTCGGATACTTAGGTGGTGGTGGAGGTCATGTTAGTAGCGATGGTGAAGTTCATGTGACACCAAACTCTTCGGTTTCATCGTCTTCAGCTGAGGCCGGCGCCGAAGAGGACTCCGGTAACAAGAGCAAGAAAGATAGGCAGCAGCCACAGCCAAAAGGGTCTTCTGAAGAAGGAGGAGATGTTCATAGCCCCAAGAAATCGAGCAAGACAGTGAAGAAGAAAGGAGAGAAAAAGCAAAGAGAGCCTCGATTTGCCTTCATGACCAAGAGTGAGGTTGATCATCTAGAAGACGGATACAGATGGAGAAAATACGGTCAGAAAGCTGTCAAAAACAGCCCCTACCCAAGAAGCTACTACCGATGCACGACGCAGAAATGTGGGGTGAAGAAGAGGGTAGAAAGGTCATTTGAGGACCCGTCGACGGTGATCACCACCTACGAGGGGCAGCACAACCATCCGATCCCGGCTACTCTCCGAGGAAGCATCAACATCAATGCAGCTCACCACCACCATGCATTGTTCTCACCTTCGTCTATGTACGCGTCTGCTGCGCCGACGACGTCGTCTTCGGGTCCTAGCTTTCCTCAGGAGTATTTGTTTCAAATGGCGACGCCTCAGATGTTGATGAGAAACGACGGAGGAGGCGCCGGCCCCGGCGGGATTTATTCCTCGAGAAATGTTAACTTTCCTCAGCAGTATCATCAGTTACCTGCAGATCAATATGGGCTTCTGCAGGACGTGGTTCCCTCTATGTTTTTCAAACAAGAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002252 GO:0002376 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0006979 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009615 GO:0009617 GO:0009620 GO:0009636 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010035 GO:0010228 GO:0010453 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0033554 GO:0033993 GO:0034599 GO:0034614 GO:0035690 GO:0042221 GO:0042493 GO:0042542 GO:0042659 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0045595 GO:0045893 GO:0045935 GO:0046677 GO:0047484 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051607 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070301 GO:0070887 GO:0071236 GO:0080090 GO:0080134 GO:0097159 GO:0097237 GO:0097305 GO:0098542 GO:0140110 GO:1901000 GO:1901002 GO:1901363 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

355

Amino Acids

38.95

Weight (kDa)

6.65

Isoelectric Point (pI)

60.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 193 - 250 5.2e-27 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012414)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29860 AT4G18170 AT5G46350
fragaria_vesca FvH4_3g06200
malus_domestica MD05G1290300.v1.1 MD10G1266400.v1.1
prunus_persica Prupe.4G075400_v2.0.a1 Prupe.4G075400_v2.0.a1
pyrus_communis pycom05g26710 pycom10g22220
rosa_chinensis RchiOBHm_Chr5g0013131
rosa_laevigata RLG00000031991
rosa_multiflora Rmu_sc0003892.1_g000004
rosa_roxburghii Rroxscaffold_1G00063450
rosa_samantha Rh5AG101600 Rh5BG098000 Rh5CG110100 Rh5DG097200
rosa_wichuraiana Rw5G008880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 853
AatII GACGTC 1 cut(s) 854
Acc36I ACCTGC 1 cut(s) 1009
AccB1I GGYRCC 2 cut(s) 389, 935
AccI GTMKAC 1 cut(s) 710
AccII CGCG 1 cut(s) 833
AciI CCGC 1 cut(s) 948
AclWI GGATC 1 cut(s) 745
AcuI CTGAAG 2 cut(s) 360, 477
AcyI GRCGYC 4 cut(s) 390, 851, 902, 936
AfaI GTAC 2 cut(s) 191, 830
AfiI CCNNNNNNNGG 2 cut(s) 712, 732
AflIII ACRYGT 1 cut(s) 831
AgsI TTSAA 2 cut(s) 893, 1051
AjiI CACGTC 1 cut(s) 1030
AjuI GAANNNNNNNTTGG 2 cut(s) 436, 468
AloI GAACNNNNNNTCC 2 cut(s) 456, 488
AluBI AGCT 7 cut(s) 128, 180, 380, 614, 642, 791, 870
AluI AGCT 7 cut(s) 128, 180, 380, 614, 642, 791, 870
AlwI GGATC 1 cut(s) 745
Ama87I CYCGRG 1 cut(s) 961
AoxI GGCC 2 cut(s) 384, 940
ApeKI GCWGC 6 cut(s) 267, 430, 433, 736, 788, 839
ArsI GACNNNNNNTTYG 2 cut(s) 841, 873
AspLEI GCGC 3 cut(s) 392, 844, 938
AspS9I GGNCC 3 cut(s) 703, 863, 941
AsuC2I CCSGG 2 cut(s) 755, 945
AsuHPI GGTGA 7 cut(s) 219, 347, 685, 712, 727, 785, 807
AvaI CYCGRG 1 cut(s) 961
AvaII GGWCC 2 cut(s) 703, 863
AxyI CCTNAGG 1 cut(s) 876
BaeI ACNNNNGTAYC 2 cut(s) 289, 322
BanI GGYRCC 2 cut(s) 389, 935
BbsI GAAGAC 4 cut(s) 366, 444, 585, 849
BbvCI CCTCAGC 2 cut(s) 381, 981
BbvI GCAGC 6 cut(s) 279, 442, 445, 748, 800, 826
BccI CCATC 4 cut(s) 279, 326, 585, 753
BceAI ACGGC 1 cut(s) 222
BciVI GTATCC 2 cut(s) 290, 578
BclI TGATCA 5 cut(s) 7, 61, 87, 568, 717
BcnI CCSGG 2 cut(s) 755, 945
BfaI CTAG 2 cut(s) 575, 867
BfmI CTRYAG 2 cut(s) 1002, 1022
BfoI RGCGCY 2 cut(s) 393, 939
BfuAI ACCTGC 1 cut(s) 1009
BfuI GTATCC 2 cut(s) 290, 578
BisI GCNGC 6 cut(s) 268, 431, 434, 737, 789, 840
BlsI GCNGC 6 cut(s) 269, 432, 435, 738, 790, 841
Bme1390I CCNGG 2 cut(s) 755, 945
Bme18I GGWCC 2 cut(s) 703, 863
BmeT110I CYCGRG 1 cut(s) 961
BmgBI CACGTC 1 cut(s) 1030
BmgT120I GGNCC 3 cut(s) 703, 863, 941
BmiI GGNNCC 6 cut(s) 391, 705, 864, 937, 943, 1035
BmrFI CCNGG 2 cut(s) 755, 945
BmsI GCATC 2 cut(s) 641, 783
BpiI GAAGAC 4 cut(s) 366, 444, 585, 849
BplI GAGNNNNNCTC 2 cut(s) 179, 211
Bpu10I CCTNAGC 2 cut(s) 381, 981
BpuMI CCSGG 2 cut(s) 755, 945
BsaBI GATNNNNATC 1 cut(s) 12
BsaHI GRCGYC 4 cut(s) 390, 851, 902, 936
BsaJI CCNNGG 2 cut(s) 766, 943
BsaWI WCCGGW 1 cut(s) 404
BsaXI ACNNNNNCTCC 6 cut(s) 306, 336, 456, 486, 872, 902
Bsc4I CCNNNNNNNGG 2 cut(s) 712, 732
Bse118I RCCGGY 2 cut(s) 386, 938
Bse21I CCTNAGG 1 cut(s) 876
Bse8I GATNNNNATC 1 cut(s) 12
BseDI CCNNGG 2 cut(s) 766, 943
BseGI GGATG 2 cut(s) 119, 745
BseJI GATNNNNATC 1 cut(s) 12
BseLI CCNNNNNNNGG 2 cut(s) 712, 732
BseMII CTCAG 4 cut(s) 372, 890, 920, 995
BseRI GAGGAG 2 cut(s) 480, 945
BseXI GCAGC 6 cut(s) 279, 442, 445, 748, 800, 826
BseYI CCCAGC 1 cut(s) 124
Bsh1236I CGCG 1 cut(s) 833
BshFI GGCC 2 cut(s) 386, 942
BshNI GGYRCC 2 cut(s) 389, 935
BsiHKCI CYCGRG 1 cut(s) 961
BsiSI CCGG 5 cut(s) 387, 405, 755, 939, 945
BslI CCNNNNNNNGG 2 cut(s) 712, 732
BsnI GGCC 2 cut(s) 386, 942
BsoBI CYCGRG 1 cut(s) 961
Bsp143I GATC 8 cut(s) 7, 61, 87, 235, 568, 717, 750, 1006
BspACI CCGC 1 cut(s) 948
BspANI GGCC 2 cut(s) 386, 942
BspCNI CTCAG 4 cut(s) 373, 889, 919, 994
BspFNI CGCG 1 cut(s) 833
BspHI TCATGA 3 cut(s) 16, 90, 549
BspLI GGNNCC 6 cut(s) 391, 705, 864, 937, 943, 1035
BspMAI CTGCAG 2 cut(s) 1006, 1026
BspMI ACCTGC 1 cut(s) 1009
BspPI GGATC 1 cut(s) 745
BspT107I GGYRCC 2 cut(s) 389, 935
BsrFI RCCGGY 2 cut(s) 386, 938
BssAI RCCGGY 2 cut(s) 386, 938
BssECI CCNNGG 2 cut(s) 766, 943
BssMI GATC 8 cut(s) 7, 61, 87, 235, 568, 717, 750, 1006
BssNI GRCGYC 4 cut(s) 390, 851, 902, 936
Bst4CI ACNGT 3 cut(s) 502, 605, 715
Bst6I CTCTTC 3 cut(s) 364, 390, 674
BstACI GRCGYC 4 cut(s) 390, 851, 902, 936
BstC8I GCNNGC 2 cut(s) 388, 940
BstDEI CTNAG 5 cut(s) 301, 381, 876, 906, 981
BstF5I GGATG 2 cut(s) 119, 745
BstFNI CGCG 1 cut(s) 833
BstH2I RGCGCY 2 cut(s) 393, 939
BstHHI GCGC 3 cut(s) 392, 844, 938
BstKTI GATC 8 cut(s) 10, 64, 90, 238, 571, 720, 753, 1009
BstMBI GATC 8 cut(s) 7, 61, 87, 235, 568, 717, 750, 1006
BstMWI GCNNNNNNNGC 3 cut(s) 439, 532, 839
BstSCI CCNGG 2 cut(s) 753, 943
BstSFI CTRYAG 2 cut(s) 1002, 1022
BstUI CGCG 1 cut(s) 833
BstV1I GCAGC 6 cut(s) 279, 442, 445, 748, 800, 826
BstV2I GAAGAC 4 cut(s) 366, 444, 585, 849
Bsu36I CCTNAGG 1 cut(s) 876
BsuI GTATCC 2 cut(s) 290, 578
BsuRI GGCC 2 cut(s) 386, 942
BtgZI GCGATG 1 cut(s) 345
BtrI CACGTC 1 cut(s) 1030
BtsCI GGATG 2 cut(s) 119, 745
BtsIMutI CAGTG 1 cut(s) 507
BveI ACCTGC 1 cut(s) 1009
Cac8I GCNNGC 2 cut(s) 388, 940
CciI TCATGA 3 cut(s) 16, 90, 549
CfoI GCGC 3 cut(s) 392, 844, 938
Cfr10I RCCGGY 2 cut(s) 386, 938
Cfr13I GGNCC 3 cut(s) 703, 863, 941
CseI GACGC 3 cut(s) 667, 822, 910
Csp6I GTAC 2 cut(s) 190, 829
CviAII CATG 9 cut(s) 17, 41, 91, 169, 320, 344, 550, 803, 1062
CviQI GTAC 2 cut(s) 190, 829
DdeI CTNAG 5 cut(s) 301, 381, 876, 906, 981
DinI GGCGCC 2 cut(s) 391, 937
DpnI GATC 8 cut(s) 9, 63, 89, 237, 570, 719, 752, 1008
DpnII GATC 8 cut(s) 7, 61, 87, 235, 568, 717, 750, 1006
DrdI GACNNNNNNGTC 1 cut(s) 853
DseDI GACNNNNNNGTC 1 cut(s) 853
Eam1104I CTCTTC 3 cut(s) 364, 390, 674
EarI CTCTTC 3 cut(s) 364, 390, 674
Eco47I GGWCC 2 cut(s) 703, 863
Eco57I CTGAAG 2 cut(s) 360, 477
Eco81I CCTNAGG 1 cut(s) 876
Eco88I CYCGRG 1 cut(s) 961
EcoO109I RGGNCCY 2 cut(s) 703, 863
EcoT22I ATGCAT 1 cut(s) 808
EgeI GGCGCC 2 cut(s) 391, 937
EheI GGCGCC 2 cut(s) 391, 937
FaeI CATG 9 cut(s) 20, 44, 94, 172, 323, 347, 553, 806, 1065
FatI CATG 9 cut(s) 16, 40, 90, 168, 319, 343, 549, 802, 1061
FauI CCCGC 1 cut(s) 941
FbaI TGATCA 5 cut(s) 7, 61, 87, 568, 717
FblI GTMKAC 1 cut(s) 710
Fnu4HI GCNGC 6 cut(s) 268, 431, 434, 737, 789, 840
FokI GGATG 2 cut(s) 106, 732
Fsp4HI GCNGC 6 cut(s) 268, 431, 434, 737, 789, 840
FspBI CTAG 2 cut(s) 575, 867
GlaI GCGC 3 cut(s) 391, 843, 937
GluI GCNGC 6 cut(s) 268, 431, 434, 737, 789, 840
GsaI CCCAGC 1 cut(s) 128
HaeII RGCGCY 2 cut(s) 393, 939
HaeIII GGCC 2 cut(s) 386, 942
HapII CCGG 5 cut(s) 387, 405, 755, 939, 945
HgaI GACGC 3 cut(s) 667, 822, 910
HhaI GCGC 3 cut(s) 392, 844, 938
Hin1I GRCGYC 4 cut(s) 390, 851, 902, 936
Hin1II CATG 9 cut(s) 20, 44, 94, 172, 323, 347, 553, 806, 1065
Hin6I GCGC 3 cut(s) 390, 842, 936
HinP1I GCGC 3 cut(s) 390, 842, 936
HincII GTYRAC 2 cut(s) 711, 973
HindII GTYRAC 2 cut(s) 711, 973
HinfI GANTC 1 cut(s) 401
HpaI GTTAAC 1 cut(s) 973
HpaII CCGG 5 cut(s) 387, 405, 755, 939, 945
HphI GGTGA 7 cut(s) 219, 347, 685, 712, 727, 785, 807
Hpy166II GTNNAC 2 cut(s) 711, 973
Hpy188I TCNGA 8 cut(s) 7, 235, 296, 457, 609, 750, 767, 909
Hpy188III TCNNGA 7 cut(s) 17, 91, 239, 550, 575, 878, 963
Hpy8I GTNNAC 2 cut(s) 711, 973
Hpy99I CGWCG 9 cut(s) 226, 661, 712, 715, 850, 853, 856, 904, 929
HpyAV CCTTC 5 cut(s) 171, 257, 455, 556, 828
HpyCH4III ACNGT 3 cut(s) 502, 605, 715
HpyCH4IV ACGT 2 cut(s) 851, 1029
HpyCH4V TGCA 6 cut(s) 203, 654, 788, 806, 1004, 1024
HpyF10VI GCNNNNNNNGC 3 cut(s) 439, 532, 839
HpyF3I CTNAG 5 cut(s) 301, 381, 876, 906, 981
HpySE526I ACGT 2 cut(s) 851, 1029
Hsp92I GRCGYC 4 cut(s) 390, 851, 902, 936
Hsp92II CATG 9 cut(s) 20, 44, 94, 172, 323, 347, 553, 806, 1065
HspAI GCGC 3 cut(s) 390, 842, 936
KasI GGCGCC 2 cut(s) 389, 935
KroI GCCGGC 2 cut(s) 386, 938
KroNI GCCGGC 2 cut(s) 388, 940
Ksp22I TGATCA 5 cut(s) 7, 61, 87, 568, 717
KspAI GTTAAC 1 cut(s) 973
Kzo9I GATC 8 cut(s) 7, 61, 87, 235, 568, 717, 750, 1006
Lsp1109I GCAGC 6 cut(s) 279, 442, 445, 748, 800, 826
LweI GCATC 2 cut(s) 641, 783
MaeI CTAG 2 cut(s) 575, 867
MaeII ACGT 2 cut(s) 851, 1029
MaeIII GTNAC 4 cut(s) 225, 346, 407, 996
MalI GATC 8 cut(s) 9, 63, 89, 237, 570, 719, 752, 1008
MboI GATC 8 cut(s) 7, 61, 87, 235, 568, 717, 750, 1006
MluI ACGCGT 1 cut(s) 831
Mly113I GGCGCC 2 cut(s) 390, 936
MlyI GAGTC 1 cut(s) 395
Mph1103I ATGCAT 1 cut(s) 808
MroNI GCCGGC 2 cut(s) 386, 938
MseI TTAA 2 cut(s) 75, 972
MslI CAYNNNNRTG 2 cut(s) 15, 801
MspA1I CMGCKG 1 cut(s) 380
MspI CCGG 5 cut(s) 387, 405, 755, 939, 945
MspR9I CCNGG 2 cut(s) 755, 945
MvnI CGCG 1 cut(s) 833
MwoI GCNNNNNNNGC 3 cut(s) 439, 532, 839
NaeI GCCGGC 2 cut(s) 388, 940
NarI GGCGCC 2 cut(s) 390, 936
NciI CCSGG 2 cut(s) 755, 945
NdeII GATC 8 cut(s) 7, 61, 87, 235, 568, 717, 750, 1006
NgoMIV GCCGGC 2 cut(s) 386, 938
NlaIII CATG 9 cut(s) 20, 44, 94, 172, 323, 347, 553, 806, 1065
NlaIV GGNNCC 6 cut(s) 391, 705, 864, 937, 943, 1035
NmuCI GTSAC 2 cut(s) 225, 346
NsiI ATGCAT 1 cut(s) 808
PaeR7I CTCGAG 1 cut(s) 961
PagI TCATGA 3 cut(s) 16, 90, 549
PcsI WCGNNNNNNNCGW 1 cut(s) 368
PdiI GCCGGC 2 cut(s) 388, 940
PflFI GACNNNGTC 2 cut(s) 850, 853
PkrI GCNGC 6 cut(s) 269, 432, 435, 738, 790, 841
PleI GAGTC 1 cut(s) 395
PluTI GGCGCC 2 cut(s) 393, 939
PpsI GAGTC 1 cut(s) 395
PpuMI RGGWCCY 2 cut(s) 703, 863
Psp5II RGGWCCY 2 cut(s) 703, 863
PspFI CCCAGC 1 cut(s) 124
PspN4I GGNNCC 6 cut(s) 391, 705, 864, 937, 943, 1035
PspPI GGNCC 3 cut(s) 703, 863, 941
PspPPI RGGWCCY 2 cut(s) 703, 863
PstI CTGCAG 2 cut(s) 1006, 1026
PsyI GACNNNGTC 2 cut(s) 850, 853
PvuII CAGCTG 1 cut(s) 380
RsaI GTAC 2 cut(s) 191, 830
RsaNI GTAC 2 cut(s) 190, 829
RseI CAYNNNNRTG 2 cut(s) 15, 801
SalI GTCGAC 1 cut(s) 709
SaqAI TTAA 2 cut(s) 75, 972
SatI GCNGC 6 cut(s) 268, 431, 434, 737, 789, 840
Sau3AI GATC 8 cut(s) 7, 61, 87, 235, 568, 717, 750, 1006
Sau96I GGNCC 3 cut(s) 703, 863, 941
SchI GAGTC 1 cut(s) 395
ScrFI CCNGG 2 cut(s) 755, 945
SfaNI GCATC 2 cut(s) 641, 783
SfcI CTRYAG 2 cut(s) 1002, 1022
SfoI GGCGCC 2 cut(s) 391, 937
Sfr274I CTCGAG 1 cut(s) 961
SgrDI CGTCGACG 1 cut(s) 709
SinI GGWCC 2 cut(s) 703, 863
SlaI CTCGAG 1 cut(s) 961
SmiMI CAYNNNNRTG 2 cut(s) 15, 801
SmlI CTYRAG 1 cut(s) 961
SmoI CTYRAG 1 cut(s) 961
SsiI CCGC 1 cut(s) 948
SspDI GGCGCC 2 cut(s) 389, 935
SspMI CTAG 2 cut(s) 575, 867
StyD4I CCNGG 2 cut(s) 753, 943
TaaI ACNGT 3 cut(s) 502, 605, 715
TaiI ACGT 2 cut(s) 854, 1032
TaqI TCGA 4 cut(s) 491, 538, 710, 962
TatI WGTACW 1 cut(s) 189
Tru1I TTAA 2 cut(s) 75, 972
Tru9I TTAA 2 cut(s) 75, 972
TscAI CASTG 1 cut(s) 507
TseFI GTSAC 2 cut(s) 225, 346
TseI GCWGC 6 cut(s) 267, 430, 433, 736, 788, 839
Tsp45I GTSAC 2 cut(s) 225, 346
TspGWI ACGGA 3 cut(s) 81, 597, 942
TspRI CASTG 1 cut(s) 507
Tth111I GACNNNGTC 2 cut(s) 850, 853
VpaK11BI GGWCC 2 cut(s) 703, 863
XbaI TCTAGA 1 cut(s) 574
XhoI CTCGAG 1 cut(s) 961
XmiI GTMKAC 1 cut(s) 710
XspI CTAG 2 cut(s) 575, 867
ZraI GACGTC 1 cut(s) 852
Zsp2I ATGCAT 1 cut(s) 808
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.