RchiOBHm_Chr5g0013131
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0012414
Physical Location & Seq
Forward (+)
8899828 .. 8902419
2592 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29367

Sequence Viewer

Length: 1062 bp
ATGTCTGATCATCATCATGAACCTAAAGACCTTTACTACCATGACCTATTTCAGTATGATCACGGACAACTTAATGGTGGAATGATCATGAACAACCCAAAAGTTTCTGGTTCATCATCCCCAGCTTACAATAATCTATTACAGGGGTTTGATGTTCCTTCATACATGAACACCAGCTTCACAGAGTACAACTCGCTTGCAACGGCTTTCGGCTTATCGTCTTCACCCTCCGATCACGAAGTATTTTCCTCCATTGACGAAGGCAGCCATCAAAAGCCTGTTGATAATCTCGGATACTTAGGTGGTGGTGGAGGTCATGTTAGTAGCGATGGTGAAGTTCATGTGACACCAAACTCTTCGGTTTCATCGTCTTCAGCTGAGGCCGGCGCCGAAGAGGACTCCGGTAACAAGAGCAAGAAAGATAGGCAGCAGCCACAGCCAAAAGGGTCTTCTGAAGAAGGAGGAGATGTTCATAGCCCCAAGAAATCGAGCAAGATAGTGAAGAAGAAAGGAGAGAAAAAGCAAAGAGAGCCTCGATTTGCCTTCATGACCAAGAGTGAGGTTGATCATCTAGAAGACGGATACAGATGGAGAAAATACGGTCAGAAAGCTGTCAAAAACAGCCCCTACCCTAGAAGCTACTACCGATGCACGACGCAGAAATGTGGGGTGAAGAAGAGGGTAGAAAGGTCATTTGAGGACCCGTCGACGGTGATCACCACCTACGAGGGGCAGCACAACCATCCGATCCCGGCTACTCTCCGAGGAAGCATCAACATCAATGCAGCTCACCACCACCATGCATTGTTCTCACCTTCGTCTATGTACGCGTCTGCTGCGCCGACCACGTCGTCTTCGGGTCCTAGCTTTCCTCAGGAGTATTTGTTTCAGATGGCGACGCCTCAGATGATGATGAGAAACGACGGAGGAGGCGCCGGCCCCGGCGGGATTTATTCCTCGAGAAATGTTAACTTTCCTCAGCAGTATCATCAGTTACCTGCAGATCAATATGGGCTTTTGCAGGACGTGGTTCCCTCTATGTTTTTCAAACACGAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002252 GO:0002376 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0006979 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009615 GO:0009617 GO:0009620 GO:0009636 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010035 GO:0010228 GO:0010453 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0033554 GO:0033993 GO:0034599 GO:0034614 GO:0035690 GO:0042221 GO:0042493 GO:0042542 GO:0042659 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0045595 GO:0045893 GO:0045935 GO:0046677 GO:0047484 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051607 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070301 GO:0070887 GO:0071236 GO:0080090 GO:0080134 GO:0097159 GO:0097237 GO:0097305 GO:0098542 GO:0140110 GO:1901000 GO:1901002 GO:1901363 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

353

Amino Acids

38.87

Weight (kDa)

6.83

Isoelectric Point (pI)

58.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 192 - 249 5.2e-27 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012414)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29860 AT4G18170 AT5G46350
fragaria_vesca FvH4_3g06200
malus_domestica MD05G1290300.v1.1 MD10G1266400.v1.1
prunus_persica Prupe.4G075400_v2.0.a1 Prupe.4G075400_v2.0.a1
pyrus_communis pycom05g26710 pycom10g22220
rosa_chinensis RchiOBHm_Chr5g0013131
rosa_laevigata RLG00000031991
rosa_multiflora Rmu_sc0003892.1_g000004
rosa_roxburghii Rroxscaffold_1G00063450
rosa_samantha Rh5AG101600 Rh5BG098000 Rh5CG110100 Rh5DG097200
rosa_wichuraiana Rw5G008880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 850
Acc36I ACCTGC 1 cut(s) 1006
AccB1I GGYRCC 2 cut(s) 386, 932
AccI GTMKAC 1 cut(s) 707
AccII CGCG 1 cut(s) 830
AciI CCGC 1 cut(s) 945
AclWI GGATC 1 cut(s) 742
AcuI CTGAAG 2 cut(s) 357, 474
AcyI GRCGYC 3 cut(s) 387, 899, 933
AfaI GTAC 2 cut(s) 188, 827
AfiI CCNNNNNNNGG 2 cut(s) 709, 729
AflIII ACRYGT 1 cut(s) 828
AgsI TTSAA 1 cut(s) 1048
AjiI CACGTC 2 cut(s) 849, 1027
AjuI GAANNNNNNNTTGG 2 cut(s) 433, 465
AloI GAACNNNNNNTCC 2 cut(s) 453, 485
AluBI AGCT 7 cut(s) 125, 177, 377, 611, 639, 788, 867
AluI AGCT 7 cut(s) 125, 177, 377, 611, 639, 788, 867
AlwI GGATC 1 cut(s) 742
Ama87I CYCGRG 1 cut(s) 958
AoxI GGCC 2 cut(s) 381, 937
ApeKI GCWGC 6 cut(s) 264, 427, 430, 733, 785, 836
AspLEI GCGC 3 cut(s) 389, 841, 935
AspS9I GGNCC 3 cut(s) 700, 860, 938
AsuC2I CCSGG 2 cut(s) 752, 942
AsuHPI GGTGA 7 cut(s) 216, 344, 682, 709, 724, 782, 804
AvaI CYCGRG 1 cut(s) 958
AvaII GGWCC 2 cut(s) 700, 860
AxyI CCTNAGG 1 cut(s) 873
BaeI ACNNNNGTAYC 2 cut(s) 286, 319
BanI GGYRCC 2 cut(s) 386, 932
BauI CACGAG 1 cut(s) 1052
BbsI GAAGAC 5 cut(s) 213, 363, 441, 582, 846
BbvCI CCTCAGC 2 cut(s) 378, 978
BbvI GCAGC 6 cut(s) 276, 439, 442, 745, 797, 823
BccI CCATC 5 cut(s) 276, 323, 582, 750, 886
BceAI ACGGC 1 cut(s) 219
BciVI GTATCC 2 cut(s) 287, 575
BclI TGATCA 5 cut(s) 7, 58, 84, 565, 714
BcnI CCSGG 2 cut(s) 752, 942
BfaI CTAG 3 cut(s) 572, 633, 864
BfmI CTRYAG 1 cut(s) 999
BfoI RGCGCY 2 cut(s) 390, 936
BfuAI ACCTGC 1 cut(s) 1006
BfuI GTATCC 2 cut(s) 287, 575
BisI GCNGC 6 cut(s) 265, 428, 431, 734, 786, 837
BlsI GCNGC 6 cut(s) 266, 429, 432, 735, 787, 838
Bme1390I CCNGG 2 cut(s) 752, 942
Bme18I GGWCC 2 cut(s) 700, 860
BmeT110I CYCGRG 1 cut(s) 958
BmgBI CACGTC 2 cut(s) 849, 1027
BmgT120I GGNCC 3 cut(s) 700, 860, 938
BmiI GGNNCC 6 cut(s) 388, 702, 861, 934, 940, 1032
BmrFI CCNGG 2 cut(s) 752, 942
BmsI GCATC 2 cut(s) 638, 780
BpiI GAAGAC 5 cut(s) 213, 363, 441, 582, 846
BplI GAGNNNNNCTC 2 cut(s) 176, 208
Bpu10I CCTNAGC 2 cut(s) 378, 978
BpuMI CCSGG 2 cut(s) 752, 942
BsaBI GATNNNNATC 1 cut(s) 12
BsaHI GRCGYC 3 cut(s) 387, 899, 933
BsaJI CCNNGG 2 cut(s) 763, 940
BsaWI WCCGGW 1 cut(s) 401
BsaXI ACNNNNNCTCC 6 cut(s) 303, 333, 453, 483, 869, 899
Bsc4I CCNNNNNNNGG 2 cut(s) 709, 729
Bse118I RCCGGY 2 cut(s) 383, 935
Bse21I CCTNAGG 1 cut(s) 873
Bse8I GATNNNNATC 1 cut(s) 12
BseDI CCNNGG 2 cut(s) 763, 940
BseGI GGATG 2 cut(s) 116, 742
BseJI GATNNNNATC 1 cut(s) 12
BseLI CCNNNNNNNGG 2 cut(s) 709, 729
BseMII CTCAG 4 cut(s) 369, 887, 917, 992
BseRI GAGGAG 2 cut(s) 477, 942
BseXI GCAGC 6 cut(s) 276, 439, 442, 745, 797, 823
BseYI CCCAGC 1 cut(s) 121
Bsh1236I CGCG 1 cut(s) 830
BshFI GGCC 2 cut(s) 383, 939
BshNI GGYRCC 2 cut(s) 386, 932
BsiHKCI CYCGRG 1 cut(s) 958
BsiSI CCGG 5 cut(s) 384, 402, 752, 936, 942
BslI CCNNNNNNNGG 2 cut(s) 709, 729
BsnI GGCC 2 cut(s) 383, 939
BsoBI CYCGRG 1 cut(s) 958
Bsp143I GATC 8 cut(s) 7, 58, 84, 232, 565, 714, 747, 1003
BspACI CCGC 1 cut(s) 945
BspANI GGCC 2 cut(s) 383, 939
BspCNI CTCAG 4 cut(s) 370, 886, 916, 991
BspFNI CGCG 1 cut(s) 830
BspHI TCATGA 3 cut(s) 16, 87, 546
BspLI GGNNCC 6 cut(s) 388, 702, 861, 934, 940, 1032
BspMAI CTGCAG 1 cut(s) 1003
BspMI ACCTGC 1 cut(s) 1006
BspPI GGATC 1 cut(s) 742
BspT107I GGYRCC 2 cut(s) 386, 932
BsrFI RCCGGY 2 cut(s) 383, 935
BssAI RCCGGY 2 cut(s) 383, 935
BssECI CCNNGG 2 cut(s) 763, 940
BssMI GATC 8 cut(s) 7, 58, 84, 232, 565, 714, 747, 1003
BssNI GRCGYC 3 cut(s) 387, 899, 933
BssSI CACGAG 1 cut(s) 1052
Bst2BI CACGAG 1 cut(s) 1052
Bst4CI ACNGT 2 cut(s) 602, 712
Bst6I CTCTTC 3 cut(s) 361, 387, 671
BstACI GRCGYC 3 cut(s) 387, 899, 933
BstC8I GCNNGC 3 cut(s) 198, 385, 937
BstDEI CTNAG 5 cut(s) 298, 378, 873, 903, 978
BstF5I GGATG 2 cut(s) 116, 742
BstFNI CGCG 1 cut(s) 830
BstH2I RGCGCY 2 cut(s) 390, 936
BstHHI GCGC 3 cut(s) 389, 841, 935
BstKTI GATC 8 cut(s) 10, 61, 87, 235, 568, 717, 750, 1006
BstMBI GATC 8 cut(s) 7, 58, 84, 232, 565, 714, 747, 1003
BstMWI GCNNNNNNNGC 3 cut(s) 436, 529, 836
BstSCI CCNGG 2 cut(s) 750, 940
BstSFI CTRYAG 1 cut(s) 999
BstUI CGCG 1 cut(s) 830
BstV1I GCAGC 6 cut(s) 276, 439, 442, 745, 797, 823
BstV2I GAAGAC 5 cut(s) 213, 363, 441, 582, 846
Bsu36I CCTNAGG 1 cut(s) 873
BsuI GTATCC 2 cut(s) 287, 575
BsuRI GGCC 2 cut(s) 383, 939
BtgZI GCGATG 1 cut(s) 342
BtrI CACGTC 2 cut(s) 849, 1027
BtsCI GGATG 2 cut(s) 116, 742
BveI ACCTGC 1 cut(s) 1006
Cac8I GCNNGC 3 cut(s) 198, 385, 937
CciI TCATGA 3 cut(s) 16, 87, 546
CfoI GCGC 3 cut(s) 389, 841, 935
Cfr10I RCCGGY 2 cut(s) 383, 935
Cfr13I GGNCC 3 cut(s) 700, 860, 938
CseI GACGC 3 cut(s) 664, 819, 907
Csp6I GTAC 2 cut(s) 187, 826
CviAII CATG 9 cut(s) 17, 41, 88, 166, 317, 341, 547, 800, 1059
CviQI GTAC 2 cut(s) 187, 826
DdeI CTNAG 5 cut(s) 298, 378, 873, 903, 978
DinI GGCGCC 2 cut(s) 388, 934
DpnI GATC 8 cut(s) 9, 60, 86, 234, 567, 716, 749, 1005
DpnII GATC 8 cut(s) 7, 58, 84, 232, 565, 714, 747, 1003
DrdI GACNNNNNNGTC 1 cut(s) 850
DseDI GACNNNNNNGTC 1 cut(s) 850
Eam1104I CTCTTC 3 cut(s) 361, 387, 671
EarI CTCTTC 3 cut(s) 361, 387, 671
Eco47I GGWCC 2 cut(s) 700, 860
Eco57I CTGAAG 2 cut(s) 357, 474
Eco81I CCTNAGG 1 cut(s) 873
Eco88I CYCGRG 1 cut(s) 958
EcoO109I RGGNCCY 2 cut(s) 700, 860
EcoT22I ATGCAT 1 cut(s) 805
EgeI GGCGCC 2 cut(s) 388, 934
EheI GGCGCC 2 cut(s) 388, 934
FaeI CATG 9 cut(s) 20, 44, 91, 169, 320, 344, 550, 803, 1062
FatI CATG 9 cut(s) 16, 40, 87, 165, 316, 340, 546, 799, 1058
FauI CCCGC 1 cut(s) 938
FbaI TGATCA 5 cut(s) 7, 58, 84, 565, 714
FblI GTMKAC 1 cut(s) 707
Fnu4HI GCNGC 6 cut(s) 265, 428, 431, 734, 786, 837
FokI GGATG 2 cut(s) 103, 729
Fsp4HI GCNGC 6 cut(s) 265, 428, 431, 734, 786, 837
FspBI CTAG 3 cut(s) 572, 633, 864
GlaI GCGC 3 cut(s) 388, 840, 934
GluI GCNGC 6 cut(s) 265, 428, 431, 734, 786, 837
GsaI CCCAGC 1 cut(s) 125
HaeII RGCGCY 2 cut(s) 390, 936
HaeIII GGCC 2 cut(s) 383, 939
HapII CCGG 5 cut(s) 384, 402, 752, 936, 942
HgaI GACGC 3 cut(s) 664, 819, 907
HhaI GCGC 3 cut(s) 389, 841, 935
Hin1I GRCGYC 3 cut(s) 387, 899, 933
Hin1II CATG 9 cut(s) 20, 44, 91, 169, 320, 344, 550, 803, 1062
Hin6I GCGC 3 cut(s) 387, 839, 933
HinP1I GCGC 3 cut(s) 387, 839, 933
HincII GTYRAC 2 cut(s) 708, 970
HindII GTYRAC 2 cut(s) 708, 970
HinfI GANTC 1 cut(s) 398
HpaI GTTAAC 1 cut(s) 970
HpaII CCGG 5 cut(s) 384, 402, 752, 936, 942
HphI GGTGA 7 cut(s) 216, 344, 682, 709, 724, 782, 804
Hpy166II GTNNAC 2 cut(s) 708, 970
Hpy188I TCNGA 9 cut(s) 7, 232, 293, 454, 606, 747, 764, 891, 906
Hpy188III TCNNGA 7 cut(s) 17, 88, 236, 547, 572, 875, 960
Hpy8I GTNNAC 2 cut(s) 708, 970
Hpy99I CGWCG 6 cut(s) 658, 709, 712, 853, 901, 926
HpyAV CCTTC 5 cut(s) 168, 254, 452, 553, 825
HpyCH4III ACNGT 2 cut(s) 602, 712
HpyCH4IV ACGT 2 cut(s) 848, 1026
HpyCH4V TGCA 6 cut(s) 200, 651, 785, 803, 1001, 1021
HpyF10VI GCNNNNNNNGC 3 cut(s) 436, 529, 836
HpyF3I CTNAG 5 cut(s) 298, 378, 873, 903, 978
HpySE526I ACGT 2 cut(s) 848, 1026
Hsp92I GRCGYC 3 cut(s) 387, 899, 933
Hsp92II CATG 9 cut(s) 20, 44, 91, 169, 320, 344, 550, 803, 1062
HspAI GCGC 3 cut(s) 387, 839, 933
KasI GGCGCC 2 cut(s) 386, 932
KroI GCCGGC 2 cut(s) 383, 935
KroNI GCCGGC 2 cut(s) 385, 937
Ksp22I TGATCA 5 cut(s) 7, 58, 84, 565, 714
KspAI GTTAAC 1 cut(s) 970
Kzo9I GATC 8 cut(s) 7, 58, 84, 232, 565, 714, 747, 1003
Lsp1109I GCAGC 6 cut(s) 276, 439, 442, 745, 797, 823
LweI GCATC 2 cut(s) 638, 780
MaeI CTAG 3 cut(s) 572, 633, 864
MaeII ACGT 2 cut(s) 848, 1026
MaeIII GTNAC 3 cut(s) 343, 404, 993
MalI GATC 8 cut(s) 9, 60, 86, 234, 567, 716, 749, 1005
MboI GATC 8 cut(s) 7, 58, 84, 232, 565, 714, 747, 1003
MluI ACGCGT 1 cut(s) 828
Mly113I GGCGCC 2 cut(s) 387, 933
MlyI GAGTC 1 cut(s) 392
Mph1103I ATGCAT 1 cut(s) 805
MroNI GCCGGC 2 cut(s) 383, 935
MseI TTAA 2 cut(s) 72, 969
MslI CAYNNNNRTG 2 cut(s) 15, 798
MspA1I CMGCKG 1 cut(s) 377
MspI CCGG 5 cut(s) 384, 402, 752, 936, 942
MspR9I CCNGG 2 cut(s) 752, 942
MvnI CGCG 1 cut(s) 830
MwoI GCNNNNNNNGC 3 cut(s) 436, 529, 836
NaeI GCCGGC 2 cut(s) 385, 937
NarI GGCGCC 2 cut(s) 387, 933
NciI CCSGG 2 cut(s) 752, 942
NdeII GATC 8 cut(s) 7, 58, 84, 232, 565, 714, 747, 1003
NgoMIV GCCGGC 2 cut(s) 383, 935
NlaIII CATG 9 cut(s) 20, 44, 91, 169, 320, 344, 550, 803, 1062
NlaIV GGNNCC 6 cut(s) 388, 702, 861, 934, 940, 1032
NmuCI GTSAC 1 cut(s) 343
NsiI ATGCAT 1 cut(s) 805
PaeR7I CTCGAG 1 cut(s) 958
PagI TCATGA 3 cut(s) 16, 87, 546
PcsI WCGNNNNNNNCGW 1 cut(s) 365
PdiI GCCGGC 2 cut(s) 385, 937
PflFI GACNNNGTC 1 cut(s) 847
PkrI GCNGC 6 cut(s) 266, 429, 432, 735, 787, 838
PleI GAGTC 1 cut(s) 392
PluTI GGCGCC 2 cut(s) 390, 936
PpsI GAGTC 1 cut(s) 392
PpuMI RGGWCCY 2 cut(s) 700, 860
Psp5II RGGWCCY 2 cut(s) 700, 860
PspFI CCCAGC 1 cut(s) 121
PspN4I GGNNCC 6 cut(s) 388, 702, 861, 934, 940, 1032
PspPI GGNCC 3 cut(s) 700, 860, 938
PspPPI RGGWCCY 2 cut(s) 700, 860
PstI CTGCAG 1 cut(s) 1003
PsyI GACNNNGTC 1 cut(s) 847
PvuII CAGCTG 1 cut(s) 377
RsaI GTAC 2 cut(s) 188, 827
RsaNI GTAC 2 cut(s) 187, 826
RseI CAYNNNNRTG 2 cut(s) 15, 798
SalI GTCGAC 1 cut(s) 706
SaqAI TTAA 2 cut(s) 72, 969
SatI GCNGC 6 cut(s) 265, 428, 431, 734, 786, 837
Sau3AI GATC 8 cut(s) 7, 58, 84, 232, 565, 714, 747, 1003
Sau96I GGNCC 3 cut(s) 700, 860, 938
SchI GAGTC 1 cut(s) 392
ScrFI CCNGG 2 cut(s) 752, 942
SfaNI GCATC 2 cut(s) 638, 780
SfcI CTRYAG 1 cut(s) 999
SfoI GGCGCC 2 cut(s) 388, 934
Sfr274I CTCGAG 1 cut(s) 958
SgrDI CGTCGACG 1 cut(s) 706
SinI GGWCC 2 cut(s) 700, 860
SlaI CTCGAG 1 cut(s) 958
SmiMI CAYNNNNRTG 2 cut(s) 15, 798
SmlI CTYRAG 1 cut(s) 958
SmoI CTYRAG 1 cut(s) 958
SsiI CCGC 1 cut(s) 945
SspDI GGCGCC 2 cut(s) 386, 932
SspMI CTAG 3 cut(s) 572, 633, 864
StyD4I CCNGG 2 cut(s) 750, 940
TaaI ACNGT 2 cut(s) 602, 712
TaiI ACGT 2 cut(s) 851, 1029
TaqI TCGA 4 cut(s) 488, 535, 707, 959
TatI WGTACW 1 cut(s) 186
Tru1I TTAA 2 cut(s) 72, 969
Tru9I TTAA 2 cut(s) 72, 969
TseFI GTSAC 1 cut(s) 343
TseI GCWGC 6 cut(s) 264, 427, 430, 733, 785, 836
Tsp45I GTSAC 1 cut(s) 343
TspDTI ATGAA 9 cut(s) 33, 102, 104, 150, 182, 329, 354, 461, 535
TspGWI ACGGA 3 cut(s) 78, 594, 939
Tth111I GACNNNGTC 1 cut(s) 847
VpaK11BI GGWCC 2 cut(s) 700, 860
XbaI TCTAGA 1 cut(s) 571
XhoI CTCGAG 1 cut(s) 958
XmiI GTMKAC 1 cut(s) 707
XspI CTAG 3 cut(s) 572, 633, 864
Zsp2I ATGCAT 1 cut(s) 805
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.