MD10G1266400.v1.1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0012414
Physical Location & Seq
Reverse (-)
35935449 .. 35937762
2314 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1266400.v1.1.491

Sequence Viewer

Length: 984 bp
ATGTCTGATCAGGAACCGAAAGACCTTTACTACCATGACCTATTTCAGTATGAAGATCATGATGGTGGGATGGTCAGCATCAACAACCAGCCAAATTTGCAAGGGTTTGATGTGAATCCTTCATACATGAACTTAACAGAGTGTTTGAAAGGATCTATGGACTATAATTCACTTGCAACGGCTTTTGGCTTGTCATCTTCGACCTCTGAAGCACTATTTTCGTCTATTGAAGGCGATCAAAAGCCTGCTAATCATCTTGGATATTCAGGTGGTGGCGGAGGCGGTGATGTGGAAACTCCTTTGACGCCAAACTCTTCCATGTCGTCTTCTTCAGCTGAGGCTGGTGCTGATGAGGACTGTGGTAAGAATAAGAAAGATAGGCAGCCAAAAGGGTCATCAGAAGATGGAGGAGATAGCTCTAAGAAAGTGAGCAAACTAGCAAAGAAGAAGGGAGAGAAAAAGCAAAGAGAGCCACGATTTGCCTTCATGACCAAGAGTGAGGTTGATCATCTAGAAGATGGATACAGATGGAGAAAATATGGGCAGAAAGCTGTGAAAAATAGCCCATATCCAAGAAGCTACTACCGGTGTACGACACAAAAATGCGGGGTCAAGAAACGCGTAGAGAGGTCGTACGAGGACCCATCCACTGTGATTACGACATACGAAGGTCAGCACAACCACCCACTTCCAGCAACCCTTAGAGGAAATGCGGCTGCAGCATTGTTCCCACCTTCTATGCTAAACCTAGCACCCGCCACGCGTACATTCTCAACCTTGGCTGGTGGTGGCCCGAGCTCTGCCTTTTCTCAGGAATTCTTGTTTCAAATGCCCCAATACATGATCAGTAACAATGCTAATCAGGGATCGTCACGAAACCCTAATTATCATCTGCAGCAGTATCAACTGCCTCATGGAGCTGCTGATCAGTATGGACTTTTGCAGGACTTGGTTCCTTCCATGTTCCTCAAACAAGAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002252 GO:0002376 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0006979 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009615 GO:0009617 GO:0009620 GO:0009636 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010035 GO:0010228 GO:0010453 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0033554 GO:0033993 GO:0034599 GO:0034614 GO:0035690 GO:0042221 GO:0042493 GO:0042542 GO:0042659 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0045595 GO:0045893 GO:0045935 GO:0046677 GO:0047484 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051607 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070301 GO:0070887 GO:0071236 GO:0080090 GO:0080134 GO:0097159 GO:0097237 GO:0097305 GO:0098542 GO:0140110 GO:1901000 GO:1901002 GO:1901363 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

328

Amino Acids

35.94

Weight (kDa)

6.46

Isoelectric Point (pI)

59.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 172 - 229 1.1e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012414)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29860 AT4G18170 AT5G46350
fragaria_vesca FvH4_3g06200
malus_domestica MD05G1290300.v1.1 MD10G1266400.v1.1
prunus_persica Prupe.4G075400_v2.0.a1 Prupe.4G075400_v2.0.a1
pyrus_communis pycom05g26710 pycom10g22220
rosa_chinensis RchiOBHm_Chr5g0013131
rosa_laevigata RLG00000031991
rosa_multiflora Rmu_sc0003892.1_g000004
rosa_roxburghii Rroxscaffold_1G00063450
rosa_samantha Rh5AG101600 Rh5BG098000 Rh5CG110100 Rh5DG097200
rosa_wichuraiana Rw5G008880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 621, 763
AciI CCGC 5 cut(s) 276, 282, 606, 713, 756
AclWI GGATC 2 cut(s) 160, 874
AcsI RAATTY 2 cut(s) 94, 815
AcuI CTGAAG 2 cut(s) 228, 315
AcyI GRCGYC 1 cut(s) 305
AfaI GTAC 3 cut(s) 592, 635, 766
AflIII ACRYGT 2 cut(s) 619, 761
AgeI ACCGGT 1 cut(s) 585
AgsI TTSAA 3 cut(s) 148, 230, 827
AluBI AGCT 6 cut(s) 335, 417, 551, 579, 798, 920
AluI AGCT 6 cut(s) 335, 417, 551, 579, 798, 920
Alw21I GWGCWC 1 cut(s) 800
AlwI GGATC 2 cut(s) 160, 874
Ama87I CYCGRG 1 cut(s) 793
AoxI GGCC 1 cut(s) 790
ApeKI GCWGC 5 cut(s) 382, 716, 719, 895, 920
ApoI RAATTY 2 cut(s) 94, 815
AsiGI ACCGGT 1 cut(s) 585
AspS9I GGNCC 2 cut(s) 640, 791
AsuHPI GGTGA 1 cut(s) 296
AvaI CYCGRG 1 cut(s) 793
AvaII GGWCC 1 cut(s) 640
BanII GRGCYC 1 cut(s) 800
BbsI GAAGAC 1 cut(s) 318
Bbv12I GWGCWC 1 cut(s) 800
BbvCI CCTCAGC 1 cut(s) 336
BbvI GCAGC 5 cut(s) 394, 703, 731, 907, 907
BccI CCATC 6 cut(s) 56, 64, 398, 512, 522, 652
BceAI ACGGC 1 cut(s) 195
BcgI CGANNNNNNTGC 2 cut(s) 201, 235
BciVI GTATCC 1 cut(s) 515
BclI TGATCA 4 cut(s) 7, 505, 843, 925
BfaI CTAG 3 cut(s) 437, 512, 749
BfmI CTRYAG 2 cut(s) 717, 893
BfuI GTATCC 1 cut(s) 515
BisI GCNGC 6 cut(s) 383, 714, 717, 720, 896, 921
BlsI GCNGC 6 cut(s) 384, 715, 718, 721, 897, 922
Bme18I GGWCC 1 cut(s) 640
BmeT110I CYCGRG 1 cut(s) 793
BmgT120I GGNCC 2 cut(s) 640, 791
BmiI GGNNCC 3 cut(s) 15, 642, 954
BmsI GCATC 1 cut(s) 87
BpiI GAAGAC 1 cut(s) 318
Bpu10I CCTNAGC 1 cut(s) 336
BsaBI GATNNNNATC 1 cut(s) 114
BsaHI GRCGYC 1 cut(s) 305
BsaJI CCNNGG 1 cut(s) 777
BsaWI WCCGGW 1 cut(s) 585
Bse118I RCCGGY 1 cut(s) 585
Bse8I GATNNNNATC 1 cut(s) 114
BseDI CCNNGG 1 cut(s) 777
BseGI GGATG 2 cut(s) 75, 644
BseJI GATNNNNATC 1 cut(s) 114
BseMII CTCAG 2 cut(s) 327, 824
BseRI GAGGAG 1 cut(s) 423
BseXI GCAGC 5 cut(s) 394, 703, 731, 907, 907
Bsh1236I CGCG 2 cut(s) 621, 763
BshFI GGCC 1 cut(s) 792
BshTI ACCGGT 1 cut(s) 585
BsiHKAI GWGCWC 1 cut(s) 800
BsiHKCI CYCGRG 1 cut(s) 793
BsiSI CCGG 1 cut(s) 586
BsiWI CGTACG 1 cut(s) 633
BsnI GGCC 1 cut(s) 792
BsoBI CYCGRG 1 cut(s) 793
Bsp1286I GDGCHC 1 cut(s) 800
Bsp143I GATC 8 cut(s) 7, 55, 152, 235, 505, 843, 866, 925
BspACI CCGC 5 cut(s) 276, 282, 606, 713, 756
BspANI GGCC 1 cut(s) 792
BspCNI CTCAG 2 cut(s) 328, 823
BspFNI CGCG 2 cut(s) 621, 763
BspHI TCATGA 2 cut(s) 58, 486
BspLI GGNNCC 3 cut(s) 15, 642, 954
BspMAI CTGCAG 2 cut(s) 721, 897
BspPI GGATC 2 cut(s) 160, 874
BsrFI RCCGGY 1 cut(s) 585
BssAI RCCGGY 1 cut(s) 585
BssECI CCNNGG 1 cut(s) 777
BssMI GATC 8 cut(s) 7, 55, 152, 235, 505, 843, 866, 925
BssNI GRCGYC 1 cut(s) 305
BssT1I CCWWGG 1 cut(s) 777
Bst4CI ACNGT 2 cut(s) 359, 652
Bst6I CTCTTC 1 cut(s) 319
BstACI GRCGYC 1 cut(s) 305
BstC8I GCNNGC 1 cut(s) 246
BstDEI CTNAG 4 cut(s) 336, 420, 701, 810
BstF5I GGATG 2 cut(s) 75, 644
BstFNI CGCG 2 cut(s) 621, 763
BstKTI GATC 8 cut(s) 10, 58, 155, 238, 508, 846, 869, 928
BstMBI GATC 8 cut(s) 7, 55, 152, 235, 505, 843, 866, 925
BstMWI GCNNNNNNNGC 3 cut(s) 97, 469, 719
BstSFI CTRYAG 2 cut(s) 717, 893
BstUI CGCG 2 cut(s) 621, 763
BstV1I GCAGC 5 cut(s) 394, 703, 731, 907, 907
BstV2I GAAGAC 1 cut(s) 318
BstX2I RGATCY 1 cut(s) 152
BstYI RGATCY 1 cut(s) 152
BsuI GTATCC 1 cut(s) 515
BsuRI GGCC 1 cut(s) 792
BtsCI GGATG 2 cut(s) 75, 644
BtsIMutI CAGTG 1 cut(s) 648
Cac8I GCNNGC 1 cut(s) 246
CciI TCATGA 2 cut(s) 58, 486
Cfr10I RCCGGY 1 cut(s) 585
Cfr13I GGNCC 2 cut(s) 640, 791
CseI GACGC 1 cut(s) 313
Csp6I GTAC 3 cut(s) 591, 634, 765
CspAI ACCGGT 1 cut(s) 585
CviAII CATG 8 cut(s) 35, 59, 127, 319, 487, 841, 914, 961
CviQI GTAC 3 cut(s) 591, 634, 765
DdeI CTNAG 4 cut(s) 336, 420, 701, 810
DpnI GATC 8 cut(s) 9, 57, 154, 237, 507, 845, 868, 927
DpnII GATC 8 cut(s) 7, 55, 152, 235, 505, 843, 866, 925
Eam1104I CTCTTC 1 cut(s) 319
EarI CTCTTC 1 cut(s) 319
EciI GGCGGA 1 cut(s) 291
Ecl136II GAGCTC 1 cut(s) 798
Eco130I CCWWGG 1 cut(s) 777
Eco24I GRGCYC 1 cut(s) 800
Eco47I GGWCC 1 cut(s) 640
Eco53kI GAGCTC 1 cut(s) 798
Eco57I CTGAAG 2 cut(s) 228, 315
Eco88I CYCGRG 1 cut(s) 793
EcoICRI GAGCTC 1 cut(s) 798
EcoO109I RGGNCCY 1 cut(s) 640
EcoRI GAATTC 1 cut(s) 815
EcoT14I CCWWGG 1 cut(s) 777
EcoT38I GRGCYC 1 cut(s) 800
ErhI CCWWGG 1 cut(s) 777
FaeI CATG 8 cut(s) 38, 62, 130, 322, 490, 844, 917, 964
FatI CATG 8 cut(s) 34, 58, 126, 318, 486, 840, 913, 960
FauI CCCGC 2 cut(s) 599, 763
FbaI TGATCA 4 cut(s) 7, 505, 843, 925
Fnu4HI GCNGC 6 cut(s) 383, 714, 717, 720, 896, 921
FokI GGATG 2 cut(s) 82, 631
FriOI GRGCYC 1 cut(s) 800
Fsp4HI GCNGC 6 cut(s) 383, 714, 717, 720, 896, 921
FspBI CTAG 3 cut(s) 437, 512, 749
GluI GCNGC 6 cut(s) 383, 714, 717, 720, 896, 921
HaeIII GGCC 1 cut(s) 792
HapII CCGG 1 cut(s) 586
HgaI GACGC 1 cut(s) 313
Hin1I GRCGYC 1 cut(s) 305
Hin1II CATG 8 cut(s) 38, 62, 130, 322, 490, 844, 917, 964
HinfI GANTC 1 cut(s) 115
HpaII CCGG 1 cut(s) 586
HphI GGTGA 1 cut(s) 296
Hpy166II GTNNAC 1 cut(s) 591
Hpy188I TCNGA 3 cut(s) 7, 208, 400
Hpy188III TCNNGA 7 cut(s) 11, 59, 487, 512, 613, 812, 873
Hpy8I GTNNAC 1 cut(s) 591
HpyAV CCTTC 7 cut(s) 129, 224, 442, 493, 662, 744, 966
HpyCH4III ACNGT 2 cut(s) 359, 652
HpyCH4V TGCA 5 cut(s) 100, 176, 719, 895, 943
HpyF10VI GCNNNNNNNGC 3 cut(s) 97, 469, 719
HpyF3I CTNAG 4 cut(s) 336, 420, 701, 810
Hsp92I GRCGYC 1 cut(s) 305
Hsp92II CATG 8 cut(s) 38, 62, 130, 322, 490, 844, 917, 964
Ksp22I TGATCA 4 cut(s) 7, 505, 843, 925
Kzo9I GATC 8 cut(s) 7, 55, 152, 235, 505, 843, 866, 925
LmnI GCTCC 1 cut(s) 917
Lsp1109I GCAGC 5 cut(s) 394, 703, 731, 907, 907
LweI GCATC 1 cut(s) 87
MaeI CTAG 3 cut(s) 437, 512, 749
MaeIII GTNAC 2 cut(s) 848, 870
MalI GATC 8 cut(s) 9, 57, 154, 237, 507, 845, 868, 927
MboI GATC 8 cut(s) 7, 55, 152, 235, 505, 843, 866, 925
MboII GAAGA 8 cut(s) 65, 189, 306, 318, 321, 413, 457, 527
MflI RGATCY 1 cut(s) 152
MhlI GDGCHC 1 cut(s) 800
MluCI AATT 4 cut(s) 94, 166, 815, 883
MluI ACGCGT 2 cut(s) 619, 761
MseI TTAA 1 cut(s) 134
MslI CAYNNNNRTG 2 cut(s) 63, 601
MspA1I CMGCKG 1 cut(s) 335
MspI CCGG 1 cut(s) 586
MvnI CGCG 2 cut(s) 621, 763
MwoI GCNNNNNNNGC 3 cut(s) 97, 469, 719
NdeII GATC 8 cut(s) 7, 55, 152, 235, 505, 843, 866, 925
NlaIII CATG 8 cut(s) 38, 62, 130, 322, 490, 844, 917, 964
NlaIV GGNNCC 3 cut(s) 15, 642, 954
NmuCI GTSAC 1 cut(s) 870
PagI TCATGA 2 cut(s) 58, 486
PfeI GAWTC 1 cut(s) 115
Pfl23II CGTACG 1 cut(s) 633
PinAI ACCGGT 1 cut(s) 585
PkrI GCNGC 6 cut(s) 384, 715, 718, 721, 897, 922
PpuMI RGGWCCY 1 cut(s) 640
Psp124BI GAGCTC 1 cut(s) 800
Psp5II RGGWCCY 1 cut(s) 640
PspLI CGTACG 1 cut(s) 633
PspN4I GGNNCC 3 cut(s) 15, 642, 954
PspPI GGNCC 2 cut(s) 640, 791
PspPPI RGGWCCY 1 cut(s) 640
PstI CTGCAG 2 cut(s) 721, 897
PsuI RGATCY 1 cut(s) 152
PvuII CAGCTG 1 cut(s) 335
RsaI GTAC 3 cut(s) 592, 635, 766
RsaNI GTAC 3 cut(s) 591, 634, 765
RseI CAYNNNNRTG 2 cut(s) 63, 601
SacI GAGCTC 1 cut(s) 800
SaqAI TTAA 1 cut(s) 134
SatI GCNGC 6 cut(s) 383, 714, 717, 720, 896, 921
Sau3AI GATC 8 cut(s) 7, 55, 152, 235, 505, 843, 866, 925
Sau96I GGNCC 2 cut(s) 640, 791
SduI GDGCHC 1 cut(s) 800
SfaNI GCATC 1 cut(s) 87
SfcI CTRYAG 2 cut(s) 717, 893
SinI GGWCC 1 cut(s) 640
SmiMI CAYNNNNRTG 2 cut(s) 63, 601
Sse9I AATT 4 cut(s) 94, 166, 815, 883
SsiI CCGC 5 cut(s) 276, 282, 606, 713, 756
SspMI CTAG 3 cut(s) 437, 512, 749
SstI GAGCTC 1 cut(s) 800
StyI CCWWGG 1 cut(s) 777
TaaI ACNGT 2 cut(s) 359, 652
TaqI TCGA 1 cut(s) 200
TasI AATT 4 cut(s) 94, 166, 815, 883
TauI GCSGC 1 cut(s) 716
TfiI GAWTC 1 cut(s) 115
Tru1I TTAA 1 cut(s) 134
Tru9I TTAA 1 cut(s) 134
TscAI CASTG 1 cut(s) 655
TseFI GTSAC 1 cut(s) 870
TseI GCWGC 5 cut(s) 382, 716, 719, 895, 920
Tsp45I GTSAC 1 cut(s) 870
TspDTI ATGAA 4 cut(s) 66, 111, 143, 475
TspRI CASTG 1 cut(s) 655
VpaK11BI GGWCC 1 cut(s) 640
XapI RAATTY 2 cut(s) 94, 815
XbaI TCTAGA 1 cut(s) 511
XspI CTAG 3 cut(s) 437, 512, 749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.