Prupe.4G075400_v2.0.a1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0012414
Physical Location & Seq
Forward (+)
3694563 .. 3698010
3448 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G075400.2

Sequence Viewer

Length: 1014 bp
ATGTCTGATCATGAACATAAAGATCTTTACTACCATGACCTATTTCAGTACGAAGATCATGAACACCTCAATGGCGGGATGATAAGCATCAATCACAACCAAAACTTGCAAGGGATTGAAGTTCCTTCATACATGAACAACACCAGCTTTGCAGAGTGTTTGAAAGGACCTATGGATTACAATACGCTCGCAACTGCTTTTGGCCTGGCTTCTTCAACGTCTGATGAAGTTATATTTTCCTCCGCTAATGAAGGAGATCAAAAGCCTGCTGAGCATATTCGATATTCAGGTGGTGTTGGCGATGGCGATGGTGGAGGTGGAGAAACTCCTTTGACACCAAACTCTTCGGTGTCGTCTTCTTCAGCTGAGGCCGGTGCCGAAGAGGACTCTGGTAAGAGTAAGAAAGACAGGCAACCAAAAGGGTCATCAGAAGATGGAGGAGATAGCTCGAAGAAAGTGATGAGCAAAGCAAAGAAGAAAGGAGAGAAAAAGCAAAGAGAGCCACGATTTGCCTTCATGACCAAGAGTGAGGTTGATCATCTAGAAGATGGATACAGATGGAGAAAATATGGGCAGAAGGCTGTGAAAAATAGTCCATATCCAAGAAGCTACTATCGATGTACGACACAAAAATGCGGGGTCAAGAAACGTGTAGAGAGGTCGTTCGAGGACCCATCCACTGTGATTACGACGTATGAAGGCCAACACAACCACCCACTCCCCGCAACTCTCAGAGGAAATGCTGCTGCTTCTTCTGCATTCTTCCCACATGCTGCTGCTGCCTTCAACTATGCGCCCATCACAGGATCTGCAGGTCCAAGCTCCAACTTTCATCAAGAACTCTTGTTTCAAATGCCCCACCACTACATGATCAATAATAACAATCAAGGGTCATCAAGAAGTGTTGTTGTTAACCCTCATCATCAGCAGCAGCATCAACTACCTCGTGCTGATCAGCATGGACTTCTGCAGGACTTGTTGTTTCCCTCCATGTTCCTCAAACAAGAGCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002252 GO:0002376 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0006979 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009615 GO:0009617 GO:0009620 GO:0009636 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010035 GO:0010228 GO:0010453 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0033554 GO:0033993 GO:0034599 GO:0034614 GO:0035690 GO:0042221 GO:0042493 GO:0042542 GO:0042659 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0045595 GO:0045893 GO:0045935 GO:0046677 GO:0047484 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051607 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070301 GO:0070887 GO:0071236 GO:0080090 GO:0080134 GO:0097159 GO:0097237 GO:0097305 GO:0098542 GO:0140110 GO:1901000 GO:1901002 GO:1901363 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

338

Amino Acids

37.28

Weight (kDa)

6.52

Isoelectric Point (pI)

54.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012414)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29860 AT4G18170 AT5G46350
fragaria_vesca FvH4_3g06200
malus_domestica MD05G1290300.v1.1 MD10G1266400.v1.1
prunus_persica Prupe.4G075400_v2.0.a1 Prupe.4G075400_v2.0.a1
pyrus_communis pycom05g26710 pycom10g22220
rosa_chinensis RchiOBHm_Chr5g0013131
rosa_laevigata RLG00000031991
rosa_multiflora Rmu_sc0003892.1_g000004
rosa_roxburghii Rroxscaffold_1G00063450
rosa_samantha Rh5AG101600 Rh5BG098000 Rh5CG110100 Rh5DG097200
rosa_wichuraiana Rw5G008880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 803
AccB1I GGYRCC 1 cut(s) 374
AciI CCGC 4 cut(s) 75, 243, 636, 723
AclWI GGATC 1 cut(s) 814
AcuI CTGAAG 1 cut(s) 345
AfaI GTAC 2 cut(s) 50, 622
AfiI CCNNNNNNNGG 1 cut(s) 803
AflIII ACRYGT 1 cut(s) 649
AgsI TTSAA 5 cut(s) 119, 163, 216, 787, 851
AjnI CCWGG 1 cut(s) 204
AluBI AGCT 5 cut(s) 147, 365, 447, 609, 822
AluI AGCT 5 cut(s) 147, 365, 447, 609, 822
AlwI GGATC 1 cut(s) 814
AlwNI CAGNNNCTG 1 cut(s) 809
AoxI GGCC 3 cut(s) 202, 369, 700
ApeKI GCWGC 7 cut(s) 743, 746, 773, 776, 779, 928, 931
AspLEI GCGC 1 cut(s) 796
AspS9I GGNCC 3 cut(s) 167, 670, 815
AvaII GGWCC 3 cut(s) 167, 670, 815
BanI GGYRCC 1 cut(s) 374
BauI CACGAG 1 cut(s) 945
BbsI GAAGAC 1 cut(s) 348
BbvCI CCTCAGC 1 cut(s) 366
BbvI GCAGC 7 cut(s) 730, 733, 760, 763, 766, 940, 943
BccI CCATC 7 cut(s) 296, 302, 428, 542, 552, 682, 806
BciT130I CCWGG 1 cut(s) 206
BciVI GTATCC 1 cut(s) 545
BclI TGATCA 4 cut(s) 7, 535, 870, 952
BfaI CTAG 1 cut(s) 542
BfmI CTRYAG 2 cut(s) 810, 968
BfuAI ACCTGC 1 cut(s) 803
BfuI GTATCC 1 cut(s) 545
BglII AGATCT 1 cut(s) 22
BisI GCNGC 7 cut(s) 744, 747, 774, 777, 780, 929, 932
BlpI GCTNAGC 1 cut(s) 270
BlsI GCNGC 7 cut(s) 745, 748, 775, 778, 781, 930, 933
Bme1390I CCNGG 1 cut(s) 206
Bme18I GGWCC 3 cut(s) 167, 670, 815
BmgT120I GGNCC 3 cut(s) 167, 670, 815
BmiI GGNNCC 2 cut(s) 376, 672
BmrFI CCNGG 1 cut(s) 206
BmsI GCATC 2 cut(s) 96, 943
BpiI GAAGAC 1 cut(s) 348
Bpu10I CCTNAGC 1 cut(s) 366
Bpu1102I GCTNAGC 1 cut(s) 270
Bsa29I ATCGAT 1 cut(s) 616
BsaBI GATNNNNATC 1 cut(s) 86
Bsc4I CCNNNNNNNGG 1 cut(s) 803
Bse118I RCCGGY 1 cut(s) 371
Bse8I GATNNNNATC 1 cut(s) 86
BseBI CCWGG 1 cut(s) 206
BseCI ATCGAT 1 cut(s) 616
BseGI GGATG 2 cut(s) 84, 674
BseJI GATNNNNATC 1 cut(s) 86
BseLI CCNNNNNNNGG 1 cut(s) 803
BseMII CTCAG 3 cut(s) 261, 357, 745
BseRI GAGGAG 1 cut(s) 453
BseXI GCAGC 7 cut(s) 730, 733, 760, 763, 766, 940, 943
BshFI GGCC 3 cut(s) 204, 371, 702
BshNI GGYRCC 1 cut(s) 374
BshVI ATCGAT 1 cut(s) 616
BsiSI CCGG 1 cut(s) 372
BslI CCNNNNNNNGG 1 cut(s) 803
BsmI GAATGC 1 cut(s) 758
BsnI GGCC 3 cut(s) 204, 371, 702
Bsp143I GATC 8 cut(s) 7, 22, 55, 256, 535, 806, 870, 952
Bsp1720I GCTNAGC 1 cut(s) 270
BspACI CCGC 4 cut(s) 75, 243, 636, 723
BspANI GGCC 3 cut(s) 204, 371, 702
BspCNI CTCAG 3 cut(s) 262, 358, 744
BspDI ATCGAT 1 cut(s) 616
BspHI TCATGA 3 cut(s) 10, 58, 516
BspLI GGNNCC 2 cut(s) 376, 672
BspMAI CTGCAG 2 cut(s) 814, 972
BspMI ACCTGC 1 cut(s) 803
BspPI GGATC 1 cut(s) 814
BspT107I GGYRCC 1 cut(s) 374
BsrFI RCCGGY 1 cut(s) 371
BssAI RCCGGY 1 cut(s) 371
BssMI GATC 8 cut(s) 7, 22, 55, 256, 535, 806, 870, 952
BssSI CACGAG 1 cut(s) 945
Bst2BI CACGAG 1 cut(s) 945
Bst2UI CCWGG 1 cut(s) 206
Bst4CI ACNGT 1 cut(s) 682
Bst6I CTCTTC 2 cut(s) 349, 375
BstC8I GCNNGC 2 cut(s) 189, 267
BstDEI CTNAG 3 cut(s) 270, 366, 731
BstF5I GGATG 2 cut(s) 84, 674
BstHHI GCGC 1 cut(s) 796
BstKTI GATC 8 cut(s) 10, 25, 58, 259, 538, 809, 873, 955
BstMBI GATC 8 cut(s) 7, 22, 55, 256, 535, 806, 870, 952
BstMWI GCNNNNNNNGC 4 cut(s) 271, 499, 755, 779
BstNI CCWGG 1 cut(s) 206
BstNSI RCATGY 1 cut(s) 773
BstSCI CCNGG 1 cut(s) 204
BstSFI CTRYAG 2 cut(s) 810, 968
BstV1I GCAGC 7 cut(s) 730, 733, 760, 763, 766, 940, 943
BstV2I GAAGAC 1 cut(s) 348
BstX2I RGATCY 2 cut(s) 22, 806
BstYI RGATCY 2 cut(s) 22, 806
Bsu15I ATCGAT 1 cut(s) 616
BsuI GTATCC 1 cut(s) 545
BsuRI GGCC 3 cut(s) 204, 371, 702
BsuTUI ATCGAT 1 cut(s) 616
BtgZI GCGATG 2 cut(s) 315, 321
BtsCI GGATG 2 cut(s) 84, 674
BtsIMutI CAGTG 1 cut(s) 678
BveI ACCTGC 1 cut(s) 803
Cac8I GCNNGC 2 cut(s) 189, 267
CaiI CAGNNNCTG 1 cut(s) 809
CciI TCATGA 3 cut(s) 10, 58, 516
CfoI GCGC 1 cut(s) 796
Cfr10I RCCGGY 1 cut(s) 371
Cfr13I GGNCC 3 cut(s) 167, 670, 815
ClaI ATCGAT 1 cut(s) 616
Csp6I GTAC 2 cut(s) 49, 621
CviAII CATG 9 cut(s) 11, 35, 59, 133, 517, 770, 868, 959, 991
CviQI GTAC 2 cut(s) 49, 621
DdeI CTNAG 3 cut(s) 270, 366, 731
DpnI GATC 8 cut(s) 9, 24, 57, 258, 537, 808, 872, 954
DpnII GATC 8 cut(s) 7, 22, 55, 256, 535, 806, 870, 952
Eam1104I CTCTTC 2 cut(s) 349, 375
EarI CTCTTC 2 cut(s) 349, 375
Eco47I GGWCC 3 cut(s) 167, 670, 815
Eco57I CTGAAG 1 cut(s) 345
EcoO109I RGGNCCY 2 cut(s) 167, 670
EcoRII CCWGG 1 cut(s) 204
FaeI CATG 9 cut(s) 14, 38, 62, 136, 520, 773, 871, 962, 994
FatI CATG 9 cut(s) 10, 34, 58, 132, 516, 769, 867, 958, 990
FauI CCCGC 3 cut(s) 68, 629, 730
FbaI TGATCA 4 cut(s) 7, 535, 870, 952
Fnu4HI GCNGC 7 cut(s) 744, 747, 774, 777, 780, 929, 932
FokI GGATG 2 cut(s) 91, 661
Fsp4HI GCNGC 7 cut(s) 744, 747, 774, 777, 780, 929, 932
FspBI CTAG 1 cut(s) 542
GlaI GCGC 1 cut(s) 795
GluI GCNGC 7 cut(s) 744, 747, 774, 777, 780, 929, 932
HaeIII GGCC 3 cut(s) 204, 371, 702
HapII CCGG 1 cut(s) 372
HhaI GCGC 1 cut(s) 796
Hin1II CATG 9 cut(s) 14, 38, 62, 136, 520, 773, 871, 962, 994
Hin6I GCGC 1 cut(s) 794
HinP1I GCGC 1 cut(s) 794
HincII GTYRAC 1 cut(s) 913
HindII GTYRAC 1 cut(s) 913
HinfI GANTC 1 cut(s) 386
HpaI GTTAAC 1 cut(s) 913
HpaII CCGG 1 cut(s) 372
Hpy166II GTNNAC 1 cut(s) 913
Hpy188I TCNGA 4 cut(s) 7, 223, 430, 734
Hpy188III TCNNGA 7 cut(s) 11, 59, 517, 542, 643, 836, 897
Hpy8I GTNNAC 1 cut(s) 913
Hpy99I CGWCG 1 cut(s) 694
HpyAV CCTTC 6 cut(s) 135, 245, 523, 571, 692, 793
HpyCH4III ACNGT 1 cut(s) 682
HpyCH4IV ACGT 3 cut(s) 218, 649, 692
HpyCH4V TGCA 5 cut(s) 109, 152, 758, 812, 970
HpyF10VI GCNNNNNNNGC 4 cut(s) 271, 499, 755, 779
HpyF3I CTNAG 3 cut(s) 270, 366, 731
HpySE526I ACGT 3 cut(s) 218, 649, 692
Hsp92II CATG 9 cut(s) 14, 38, 62, 136, 520, 773, 871, 962, 994
HspAI GCGC 1 cut(s) 794
Ksp22I TGATCA 4 cut(s) 7, 535, 870, 952
KspAI GTTAAC 1 cut(s) 913
Kzo9I GATC 8 cut(s) 7, 22, 55, 256, 535, 806, 870, 952
LmnI GCTCC 1 cut(s) 827
Lsp1109I GCAGC 7 cut(s) 730, 733, 760, 763, 766, 940, 943
LweI GCATC 2 cut(s) 96, 943
MaeI CTAG 1 cut(s) 542
MaeII ACGT 3 cut(s) 218, 649, 692
MalI GATC 8 cut(s) 9, 24, 57, 258, 537, 808, 872, 954
MboI GATC 8 cut(s) 7, 22, 55, 256, 535, 806, 870, 952
MflI RGATCY 2 cut(s) 22, 806
MlyI GAGTC 1 cut(s) 380
MmeI TCCRAC 1 cut(s) 849
MseI TTAA 1 cut(s) 912
MslI CAYNNNNRTG 2 cut(s) 69, 631
MspA1I CMGCKG 1 cut(s) 365
MspI CCGG 1 cut(s) 372
MspR9I CCNGG 1 cut(s) 206
Mva1269I GAATGC 1 cut(s) 758
MvaI CCWGG 1 cut(s) 206
MwoI GCNNNNNNNGC 4 cut(s) 271, 499, 755, 779
NdeII GATC 8 cut(s) 7, 22, 55, 256, 535, 806, 870, 952
NlaIII CATG 9 cut(s) 14, 38, 62, 136, 520, 773, 871, 962, 994
NlaIV GGNNCC 2 cut(s) 376, 672
NspI RCATGY 1 cut(s) 773
PagI TCATGA 3 cut(s) 10, 58, 516
PctI GAATGC 1 cut(s) 758
PkrI GCNGC 7 cut(s) 745, 748, 775, 778, 781, 930, 933
PleI GAGTC 1 cut(s) 380
PpsI GAGTC 1 cut(s) 380
PpuMI RGGWCCY 2 cut(s) 167, 670
Psp5II RGGWCCY 2 cut(s) 167, 670
Psp6I CCWGG 1 cut(s) 204
PspGI CCWGG 1 cut(s) 204
PspN4I GGNNCC 2 cut(s) 376, 672
PspPI GGNCC 3 cut(s) 167, 670, 815
PspPPI RGGWCCY 2 cut(s) 167, 670
PstI CTGCAG 2 cut(s) 814, 972
PstNI CAGNNNCTG 1 cut(s) 809
PsuI RGATCY 2 cut(s) 22, 806
PvuII CAGCTG 1 cut(s) 365
RsaI GTAC 2 cut(s) 50, 622
RsaNI GTAC 2 cut(s) 49, 621
RseI CAYNNNNRTG 2 cut(s) 69, 631
SaqAI TTAA 1 cut(s) 912
SatI GCNGC 7 cut(s) 744, 747, 774, 777, 780, 929, 932
Sau3AI GATC 8 cut(s) 7, 22, 55, 256, 535, 806, 870, 952
Sau96I GGNCC 3 cut(s) 167, 670, 815
SchI GAGTC 1 cut(s) 380
ScrFI CCNGG 1 cut(s) 206
SfaNI GCATC 2 cut(s) 96, 943
SfcI CTRYAG 2 cut(s) 810, 968
SinI GGWCC 3 cut(s) 167, 670, 815
SmiMI CAYNNNNRTG 2 cut(s) 69, 631
SsiI CCGC 4 cut(s) 75, 243, 636, 723
SspMI CTAG 1 cut(s) 542
StyD4I CCNGG 1 cut(s) 204
TaaI ACNGT 1 cut(s) 682
TaiI ACGT 3 cut(s) 221, 652, 695
TaqI TCGA 4 cut(s) 280, 449, 616, 666
Tru1I TTAA 1 cut(s) 912
Tru9I TTAA 1 cut(s) 912
TscAI CASTG 1 cut(s) 685
TseI GCWGC 7 cut(s) 743, 746, 773, 776, 779, 928, 931
TspDTI ATGAA 9 cut(s) 27, 75, 117, 149, 240, 264, 505, 711, 821
TspRI CASTG 1 cut(s) 685
VpaK11BI GGWCC 3 cut(s) 167, 670, 815
XbaI TCTAGA 1 cut(s) 541
XceI RCATGY 1 cut(s) 773
XspI CTAG 1 cut(s) 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.