FvH4_3g06200
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0012414
Physical Location & Seq
Reverse (-)
3562092 .. 3564750
2659 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g06200.t1

Sequence Viewer

Length: 1068 bp
ATGTCTGATCATCATCATGAGCCTAAAGATCTTTACTACCATGATCTATTTCAGTATGATGATCATGGACAACTTAACGGTGGAATGATCATGAACAGCCCCAAAGTTTCTGGTTCATCATCCCCGGCTTACAATAATATTAATCCATTACAGGGGTTTGATGTTCCTTCATACTTGAACACCAGCTTCACAGAGTACAGTTCACTTGCAACGGCTTTTGGCTTATCGTCTTCAACCTCCGATCATGAAGTATTCTCCTCCATTGATGAAGGCAACCATCAAAAGCCTGCTGATAATAATCTCGGATACTTACGTGGTGGTGGTGGAGGTCATGTAAGTAGCGATGGTGAAGTTCTTATGACACCAAACTCCTCGGTATCTTCCTCTTCAGGTGAGGCCGGCGCCGAAGAGGGGGACTCCGGTAACAAGAACAAGAAAGACAGGCAGAAGCCAAAAGGGTCTTCGGAAGAAGGAGGAGATGTCCATAGCCCCAAGAAATCGAGCAAGATAGTGAAGAAGAAAGGAGAGAAGAAGCAAAGAGAGCCTCGATTTGCTTTCATGACCAAGAGTGAGGTTGATCATCTAGAAGACGGATACAGATGGAGAAAATATGGTCAGAAAGCTGTCAAAAATAGCCCATACCCAAGAAGCTACTACCGATGCACGACGCAGAAATGTGGGGTGAAGAAGAGGGTAGAAAGGTCATTTGAGGACCCGTCGACGGTGATCACCACCTACGAGGGGCAGCACAACCATCCGATCCCGGCGACTCTCCGAGGAAGCATCAACATCAACGCGGCTCACCACCACCATGCATTCTTCTCTCCTCAGTCTATGTACGCGTCTGCTGCCACGACGACGTCGTCTTCCGGTCCTAGCTTTCCTCAAGAGTATCTGTTTCAGATGGCGCAGCAGCCTCAGATGATGATGAGAAACGACGGAGGAGGCGGCGGGGTTTATACGTCGAGGAATGTTAACGTTCCTCAACAGTACCATCAGTTATCTGGAGATCAATACGGGCTATTGCAGGACGTGGTTCCCTCGATGTTTTTCAAACAGGAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002252 GO:0002376 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0006979 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009615 GO:0009617 GO:0009620 GO:0009636 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010035 GO:0010228 GO:0010453 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0033554 GO:0033993 GO:0034599 GO:0034614 GO:0035690 GO:0042221 GO:0042493 GO:0042542 GO:0042659 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0045595 GO:0045893 GO:0045935 GO:0046677 GO:0047484 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051607 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070301 GO:0070887 GO:0071236 GO:0080090 GO:0080134 GO:0097159 GO:0097237 GO:0097305 GO:0098542 GO:0140110 GO:1901000 GO:1901002 GO:1901363 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

356

Amino Acids

39.18

Weight (kDa)

7.0

Isoelectric Point (pI)

58.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 196 - 253 5.2e-27 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012414)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29860 AT4G18170 AT5G46350
fragaria_vesca FvH4_3g06200
malus_domestica MD05G1290300.v1.1 MD10G1266400.v1.1
prunus_persica Prupe.4G075400_v2.0.a1 Prupe.4G075400_v2.0.a1
pyrus_communis pycom05g26710 pycom10g22220
rosa_chinensis RchiOBHm_Chr5g0013131
rosa_laevigata RLG00000031991
rosa_multiflora Rmu_sc0003892.1_g000004
rosa_roxburghii Rroxscaffold_1G00063450
rosa_samantha Rh5AG101600 Rh5BG098000 Rh5CG110100 Rh5DG097200
rosa_wichuraiana Rw5G008880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 862
AatII GACGTC 1 cut(s) 863
AccB1I GGYRCC 1 cut(s) 401
AccI GTMKAC 1 cut(s) 719
AccII CGCG 2 cut(s) 797, 842
AciI CCGC 3 cut(s) 797, 948, 951
AclI AACGTT 1 cut(s) 978
AclWI GGATC 1 cut(s) 754
AcuI CTGAAG 1 cut(s) 372
AcyI GRCGYC 2 cut(s) 402, 860
AfaI GTAC 3 cut(s) 197, 839, 992
AfiI CCNNNNNNNGG 4 cut(s) 152, 411, 721, 741
AflIII ACRYGT 1 cut(s) 840
AgsI TTSAA 3 cut(s) 178, 234, 1054
AjiI CACGTC 1 cut(s) 1033
AjuI GAANNNNNNNTTGG 2 cut(s) 445, 477
AluBI AGCT 4 cut(s) 186, 623, 651, 879
AluI AGCT 4 cut(s) 186, 623, 651, 879
AlwI GGATC 1 cut(s) 754
AoxI GGCC 1 cut(s) 396
ApeKI GCWGC 4 cut(s) 745, 848, 910, 913
AseI ATTAAT 1 cut(s) 141
AspLEI GCGC 2 cut(s) 404, 910
AspS9I GGNCC 2 cut(s) 712, 872
AsuC2I CCSGG 2 cut(s) 125, 764
AsuHPI GGTGA 6 cut(s) 359, 404, 694, 721, 736, 794
AvaII GGWCC 2 cut(s) 712, 872
BaeI ACNNNNGTAYC 2 cut(s) 298, 331
BanI GGYRCC 1 cut(s) 401
BbsI GAAGAC 4 cut(s) 222, 453, 594, 858
BbvI GCAGC 4 cut(s) 757, 835, 922, 925
BccI CCATC 6 cut(s) 285, 338, 594, 762, 898, 1002
BceAI ACGGC 1 cut(s) 228
BciVI GTATCC 2 cut(s) 299, 587
BclI TGATCA 5 cut(s) 7, 61, 87, 577, 726
BcnI CCSGG 2 cut(s) 125, 764
BfaI CTAG 2 cut(s) 584, 876
BfoI RGCGCY 1 cut(s) 405
BfuI GTATCC 2 cut(s) 299, 587
BglII AGATCT 1 cut(s) 28
BisI GCNGC 6 cut(s) 746, 798, 849, 911, 914, 949
BlsI GCNGC 6 cut(s) 747, 799, 850, 912, 915, 950
Bme1390I CCNGG 2 cut(s) 125, 764
Bme18I GGWCC 2 cut(s) 712, 872
BmgBI CACGTC 1 cut(s) 1033
BmgT120I GGNCC 2 cut(s) 712, 872
BmiI GGNNCC 4 cut(s) 403, 714, 1038, 1062
BmrFI CCNGG 2 cut(s) 125, 764
BmsI GCATC 2 cut(s) 650, 792
BpiI GAAGAC 4 cut(s) 222, 453, 594, 858
BplI GAGNNNNNCTC 2 cut(s) 401, 433
BpmI CTGGAG 1 cut(s) 1026
BpuEI CTTGAG 1 cut(s) 870
BpuMI CCSGG 2 cut(s) 125, 764
BsaAI YACGTR 1 cut(s) 314
BsaBI GATNNNNATC 2 cut(s) 12, 297
BsaHI GRCGYC 2 cut(s) 402, 860
BsaJI CCNNGG 3 cut(s) 123, 372, 775
BsaWI WCCGGW 2 cut(s) 419, 869
BsaXI ACNNNNNCTCC 4 cut(s) 318, 348, 465, 495
Bsc4I CCNNNNNNNGG 4 cut(s) 152, 411, 721, 741
Bse118I RCCGGY 1 cut(s) 398
Bse8I GATNNNNATC 2 cut(s) 12, 297
BseDI CCNNGG 3 cut(s) 123, 372, 775
BseGI GGATG 2 cut(s) 119, 754
BseJI GATNNNNATC 2 cut(s) 12, 297
BseLI CCNNNNNNNGG 4 cut(s) 152, 411, 721, 741
BseMII CTCAG 2 cut(s) 842, 932
BseRI GAGGAG 5 cut(s) 247, 361, 489, 816, 957
BseXI GCAGC 4 cut(s) 757, 835, 922, 925
Bsh1236I CGCG 2 cut(s) 797, 842
BshFI GGCC 1 cut(s) 398
BshNI GGYRCC 1 cut(s) 401
BsiSI CCGG 5 cut(s) 125, 399, 420, 764, 870
BslFI GGGAC 1 cut(s) 428
BslI CCNNNNNNNGG 4 cut(s) 152, 411, 721, 741
BsmFI GGGAC 1 cut(s) 428
BsmI GAATGC 1 cut(s) 815
BsnI GGCC 1 cut(s) 398
BspACI CCGC 3 cut(s) 797, 948, 951
BspANI GGCC 1 cut(s) 398
BspCNI CTCAG 2 cut(s) 841, 931
BspFNI CGCG 2 cut(s) 797, 842
BspHI TCATGA 4 cut(s) 16, 90, 244, 558
BspLI GGNNCC 4 cut(s) 403, 714, 1038, 1062
BspPI GGATC 1 cut(s) 754
BspT107I GGYRCC 1 cut(s) 401
BsrFI RCCGGY 1 cut(s) 398
BssAI RCCGGY 1 cut(s) 398
BssECI CCNNGG 3 cut(s) 123, 372, 775
BssNI GRCGYC 2 cut(s) 402, 860
Bst4CI ACNGT 4 cut(s) 80, 200, 724, 990
Bst6I CTCTTC 3 cut(s) 391, 402, 683
BstACI GRCGYC 2 cut(s) 402, 860
BstBAI YACGTR 1 cut(s) 314
BstC8I GCNNGC 2 cut(s) 288, 400
BstDEI CTNAG 2 cut(s) 828, 918
BstF5I GGATG 2 cut(s) 119, 754
BstFNI CGCG 2 cut(s) 797, 842
BstH2I RGCGCY 1 cut(s) 405
BstHHI GCGC 2 cut(s) 404, 910
BstMWI GCNNNNNNNGC 2 cut(s) 541, 848
BstSCI CCNGG 2 cut(s) 123, 762
BstUI CGCG 2 cut(s) 797, 842
BstV1I GCAGC 4 cut(s) 757, 835, 922, 925
BstV2I GAAGAC 4 cut(s) 222, 453, 594, 858
BstX2I RGATCY 1 cut(s) 28
BstYI RGATCY 1 cut(s) 28
BsuI GTATCC 2 cut(s) 299, 587
BsuRI GGCC 1 cut(s) 398
BtgZI GCGATG 1 cut(s) 357
BtrI CACGTC 1 cut(s) 1033
BtsCI GGATG 2 cut(s) 119, 754
Cac8I GCNNGC 2 cut(s) 288, 400
CciI TCATGA 4 cut(s) 16, 90, 244, 558
CfoI GCGC 2 cut(s) 404, 910
Cfr10I RCCGGY 1 cut(s) 398
Cfr13I GGNCC 2 cut(s) 712, 872
CseI GACGC 2 cut(s) 676, 831
Csp6I GTAC 3 cut(s) 196, 838, 991
CviAII CATG 9 cut(s) 17, 41, 65, 91, 245, 332, 559, 812, 1065
CviQI GTAC 3 cut(s) 196, 838, 991
DdeI CTNAG 2 cut(s) 828, 918
DinI GGCGCC 1 cut(s) 403
DrdI GACNNNNNNGTC 1 cut(s) 862
DseDI GACNNNNNNGTC 1 cut(s) 862
Eam1104I CTCTTC 3 cut(s) 391, 402, 683
EarI CTCTTC 3 cut(s) 391, 402, 683
Eco47I GGWCC 2 cut(s) 712, 872
Eco57I CTGAAG 1 cut(s) 372
EcoO109I RGGNCCY 1 cut(s) 712
EcoT22I ATGCAT 1 cut(s) 817
EgeI GGCGCC 1 cut(s) 403
EheI GGCGCC 1 cut(s) 403
FaeI CATG 9 cut(s) 20, 44, 68, 94, 248, 335, 562, 815, 1068
FaqI GGGAC 1 cut(s) 428
FatI CATG 9 cut(s) 16, 40, 64, 90, 244, 331, 558, 811, 1064
FauI CCCGC 1 cut(s) 944
FbaI TGATCA 5 cut(s) 7, 61, 87, 577, 726
FblI GTMKAC 1 cut(s) 719
Fnu4HI GCNGC 6 cut(s) 746, 798, 849, 911, 914, 949
FokI GGATG 2 cut(s) 106, 741
Fsp4HI GCNGC 6 cut(s) 746, 798, 849, 911, 914, 949
FspBI CTAG 2 cut(s) 584, 876
GlaI GCGC 2 cut(s) 403, 909
GluI GCNGC 6 cut(s) 746, 798, 849, 911, 914, 949
GsuI CTGGAG 1 cut(s) 1026
HaeII RGCGCY 1 cut(s) 405
HaeIII GGCC 1 cut(s) 398
HapII CCGG 5 cut(s) 125, 399, 420, 764, 870
HgaI GACGC 2 cut(s) 676, 831
HhaI GCGC 2 cut(s) 404, 910
Hin1I GRCGYC 2 cut(s) 402, 860
Hin1II CATG 9 cut(s) 20, 44, 68, 94, 248, 335, 562, 815, 1068
Hin6I GCGC 2 cut(s) 402, 908
HinP1I GCGC 2 cut(s) 402, 908
HincII GTYRAC 2 cut(s) 720, 976
HindII GTYRAC 2 cut(s) 720, 976
HinfI GANTC 2 cut(s) 416, 769
HpaI GTTAAC 1 cut(s) 976
HpaII CCGG 5 cut(s) 125, 399, 420, 764, 870
HphI GGTGA 6 cut(s) 359, 404, 694, 721, 736, 794
Hpy166II GTNNAC 3 cut(s) 203, 720, 976
Hpy188I TCNGA 9 cut(s) 7, 241, 305, 466, 618, 759, 776, 903, 921
Hpy188III TCNNGA 7 cut(s) 17, 91, 245, 559, 584, 887, 1005
Hpy8I GTNNAC 3 cut(s) 203, 720, 976
Hpy99I CGWCG 8 cut(s) 670, 721, 724, 859, 862, 865, 941, 967
HpyAV CCTTC 3 cut(s) 177, 263, 464
HpyCH4III ACNGT 4 cut(s) 80, 200, 724, 990
HpyCH4IV ACGT 5 cut(s) 313, 860, 962, 978, 1032
HpyCH4V TGCA 4 cut(s) 209, 663, 815, 1027
HpyF10VI GCNNNNNNNGC 2 cut(s) 541, 848
HpyF3I CTNAG 2 cut(s) 828, 918
HpySE526I ACGT 5 cut(s) 313, 860, 962, 978, 1032
Hsp92I GRCGYC 2 cut(s) 402, 860
Hsp92II CATG 9 cut(s) 20, 44, 68, 94, 248, 335, 562, 815, 1068
HspAI GCGC 2 cut(s) 402, 908
KasI GGCGCC 1 cut(s) 401
KroI GCCGGC 1 cut(s) 398
KroNI GCCGGC 1 cut(s) 400
Ksp22I TGATCA 5 cut(s) 7, 61, 87, 577, 726
KspAI GTTAAC 1 cut(s) 976
LmnI GCTCC 1 cut(s) 1060
Lsp1109I GCAGC 4 cut(s) 757, 835, 922, 925
LweI GCATC 2 cut(s) 650, 792
MaeI CTAG 2 cut(s) 584, 876
MaeII ACGT 5 cut(s) 313, 860, 962, 978, 1032
MaeIII GTNAC 1 cut(s) 422
MflI RGATCY 1 cut(s) 28
MluI ACGCGT 1 cut(s) 840
Mly113I GGCGCC 1 cut(s) 402
MlyI GAGTC 2 cut(s) 410, 763
Mph1103I ATGCAT 1 cut(s) 817
MroNI GCCGGC 1 cut(s) 398
MseI TTAA 3 cut(s) 75, 141, 975
MslI CAYNNNNRTG 2 cut(s) 15, 810
MspI CCGG 5 cut(s) 125, 399, 420, 764, 870
MspR9I CCNGG 2 cut(s) 125, 764
Mva1269I GAATGC 1 cut(s) 815
MvnI CGCG 2 cut(s) 797, 842
MwoI GCNNNNNNNGC 2 cut(s) 541, 848
NaeI GCCGGC 1 cut(s) 400
NarI GGCGCC 1 cut(s) 402
NciI CCSGG 2 cut(s) 125, 764
NgoMIV GCCGGC 1 cut(s) 398
NlaIII CATG 9 cut(s) 20, 44, 68, 94, 248, 335, 562, 815, 1068
NlaIV GGNNCC 4 cut(s) 403, 714, 1038, 1062
NsiI ATGCAT 1 cut(s) 817
PagI TCATGA 4 cut(s) 16, 90, 244, 558
PcsI WCGNNNNNNNCGW 1 cut(s) 860
PctI GAATGC 1 cut(s) 815
PdiI GCCGGC 1 cut(s) 400
PflFI GACNNNGTC 2 cut(s) 859, 862
PkrI GCNGC 6 cut(s) 747, 799, 850, 912, 915, 950
PleI GAGTC 2 cut(s) 410, 763
PluTI GGCGCC 1 cut(s) 405
PpsI GAGTC 2 cut(s) 410, 763
Ppu21I YACGTR 1 cut(s) 314
PpuMI RGGWCCY 1 cut(s) 712
PshBI ATTAAT 1 cut(s) 141
Psp1406I AACGTT 1 cut(s) 978
Psp5II RGGWCCY 1 cut(s) 712
PspN4I GGNNCC 4 cut(s) 403, 714, 1038, 1062
PspPI GGNCC 2 cut(s) 712, 872
PspPPI RGGWCCY 1 cut(s) 712
PsuI RGATCY 1 cut(s) 28
PsyI GACNNNGTC 2 cut(s) 859, 862
RsaI GTAC 3 cut(s) 197, 839, 992
RsaNI GTAC 3 cut(s) 196, 838, 991
RseI CAYNNNNRTG 2 cut(s) 15, 810
SalI GTCGAC 1 cut(s) 718
SaqAI TTAA 3 cut(s) 75, 141, 975
SatI GCNGC 6 cut(s) 746, 798, 849, 911, 914, 949
Sau96I GGNCC 2 cut(s) 712, 872
SchI GAGTC 2 cut(s) 410, 763
ScrFI CCNGG 2 cut(s) 125, 764
SfaNI GCATC 2 cut(s) 650, 792
SfoI GGCGCC 1 cut(s) 403
SgrDI CGTCGACG 1 cut(s) 718
SinI GGWCC 2 cut(s) 712, 872
SmiMI CAYNNNNRTG 2 cut(s) 15, 810
SmlI CTYRAG 1 cut(s) 885
SmoI CTYRAG 1 cut(s) 885
SsiI CCGC 3 cut(s) 797, 948, 951
SspDI GGCGCC 1 cut(s) 401
SspI AATATT 1 cut(s) 139
SspMI CTAG 2 cut(s) 584, 876
StyD4I CCNGG 2 cut(s) 123, 762
TaaI ACNGT 4 cut(s) 80, 200, 724, 990
TaiI ACGT 5 cut(s) 316, 863, 965, 981, 1035
TaqI TCGA 5 cut(s) 500, 547, 719, 965, 1043
TatI WGTACW 1 cut(s) 195
TauI GCSGC 2 cut(s) 800, 951
Tru1I TTAA 3 cut(s) 75, 141, 975
Tru9I TTAA 3 cut(s) 75, 141, 975
TseI GCWGC 4 cut(s) 745, 848, 910, 913
TspDTI ATGAA 6 cut(s) 105, 107, 159, 261, 282, 547
TspGWI ACGGA 2 cut(s) 606, 954
Tth111I GACNNNGTC 2 cut(s) 859, 862
VpaK11BI GGWCC 2 cut(s) 712, 872
VspI ATTAAT 1 cut(s) 141
XbaI TCTAGA 1 cut(s) 583
XcmI CCANNNNNNNNNTGG 1 cut(s) 1001
XmiI GTMKAC 1 cut(s) 719
XspI CTAG 2 cut(s) 584, 876
ZraI GACGTC 1 cut(s) 861
Zsp2I ATGCAT 1 cut(s) 817
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.