MD05G1290300.v1.1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0012414
Physical Location & Seq
Reverse (-)
42212192 .. 42214208
2017 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1290300.v1.1.491

Sequence Viewer

Length: 960 bp
ATGTCTGATCATGAACCTAAGGACCTCTACTACCATGACCTATTTCAGTATGAAGCTCATGAACACCTCAATGGTGGGATGGTCAGCATCAACGACCATCAAAATCTGCAGGGGTTTGATGTCAATCCTTCATACATGAACTTCACAGAGTGTTCGAAAGGATCTGTGGACTATAACTCACTTGCCACGGCTTTTGGCTTGTCATCTTCAACATCTGAAGCACTATTTTCATCCGTTGAAGGCAATCAAAACGCTGTTGATCATCTAGGAGGCGGTGGCGGTGGCGGCGGTGAAACTCCTTTGACGCCAAACTCTTCAGTGTCTTCTTCTTCAGCTGAGGCTGATGCTGATAAGGACTGTGGTAAGAATAAGAAAGATAGGCAGCCAAAAGGGTCATCAGAAGATGGAGATAGCTCTAAGAAAGTGAGCAAACCAGCAAAAAAGAAGGGAGAGAAAAAGCAAAAAGAGCCACGATTTGCCTTCATGACCAAGAGTGAGGTTGATCATCTAGAAGATGGATACAGGTGGAGAAAATATGGACAAAAAGCTGTGAAAAATAGTCCATATCCAAGAAGCTACTACCGATGCACGACACAAAAATGCGGGGTCAAGAAACGCGTAGAGAGGTCGTACGAGGACCCATCTATTGTGATTACAACGTATGAAGGCCAGCACAACCACCAACTTCCAGCAACCCTAAGAGGAAATGCGGCTGCAGCATTGTTCCCCCCTTCTATGCTAAACCTGTCGAACTTGGCTGCTGGTGGCCCGAGCTCTACCTTTCCTCAGGAACTCTTGTTTCAAATGCCCCAATACATGATCAGTAGCAATGTTAATCAGGGATCGTCACGAAACCCTAATCATCTTCTGCAGCAATATCAACTGCCTCATGCAGCTGCTGATCAGTATGGACTTTTGCAGGACTTTGTTCCTTCGATGTTCCTCAAACAAGAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002252 GO:0002376 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0006979 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009615 GO:0009617 GO:0009620 GO:0009636 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010035 GO:0010228 GO:0010453 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0033554 GO:0033993 GO:0034599 GO:0034614 GO:0035690 GO:0042221 GO:0042493 GO:0042542 GO:0042659 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0045595 GO:0045893 GO:0045935 GO:0046677 GO:0047484 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051607 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070301 GO:0070887 GO:0071236 GO:0080090 GO:0080134 GO:0097159 GO:0097237 GO:0097305 GO:0098542 GO:0140110 GO:1901000 GO:1901002 GO:1901363 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

320

Amino Acids

35.12

Weight (kDa)

6.6

Isoelectric Point (pI)

51.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 171 - 228 9.3e-27 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012414)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29860 AT4G18170 AT5G46350
fragaria_vesca FvH4_3g06200
malus_domestica MD05G1290300.v1.1 MD10G1266400.v1.1
prunus_persica Prupe.4G075400_v2.0.a1 Prupe.4G075400_v2.0.a1
pyrus_communis pycom05g26710 pycom10g22220
rosa_chinensis RchiOBHm_Chr5g0013131
rosa_laevigata RLG00000031991
rosa_multiflora Rmu_sc0003892.1_g000004
rosa_roxburghii Rroxscaffold_1G00063450
rosa_samantha Rh5AG101600 Rh5BG098000 Rh5CG110100 Rh5DG097200
rosa_wichuraiana Rw5G008880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 618
AciI CCGC 6 cut(s) 273, 279, 285, 288, 603, 710
AclWI GGATC 2 cut(s) 169, 850
AcuI CTGAAG 3 cut(s) 237, 300, 315
AcyI GRCGYC 1 cut(s) 305
AdeI CACNNNGTG 1 cut(s) 150
AfaI GTAC 1 cut(s) 632
AflIII ACRYGT 1 cut(s) 616
AgsI TTSAA 3 cut(s) 210, 239, 803
AluBI AGCT 7 cut(s) 56, 335, 414, 548, 576, 774, 896
AluI AGCT 7 cut(s) 56, 335, 414, 548, 576, 774, 896
Alw21I GWGCWC 1 cut(s) 776
AlwI GGATC 2 cut(s) 169, 850
AlwNI CAGNNNCTG 1 cut(s) 899
Ama87I CYCGRG 1 cut(s) 769
AoxI GGCC 2 cut(s) 667, 766
ApeKI GCWGC 7 cut(s) 382, 713, 716, 758, 871, 893, 896
AspS9I GGNCC 3 cut(s) 22, 637, 767
AsuHPI GGTGA 1 cut(s) 302
AsuII TTCGAA 1 cut(s) 155
AvaI CYCGRG 1 cut(s) 769
AvaII GGWCC 2 cut(s) 22, 637
AxyI CCTNAGG 2 cut(s) 18, 786
BanII GRGCYC 1 cut(s) 776
BbsI GAAGAC 1 cut(s) 315
Bbv12I GWGCWC 1 cut(s) 776
BbvCI CCTCAGC 1 cut(s) 336
BbvI GCAGC 7 cut(s) 394, 700, 728, 745, 883, 883, 905
BccI CCATC 5 cut(s) 73, 105, 398, 509, 649
BceAI ACGGC 1 cut(s) 204
BciVI GTATCC 1 cut(s) 512
BclI TGATCA 5 cut(s) 7, 259, 502, 819, 901
BfaI CTAG 2 cut(s) 266, 509
BfmI CTRYAG 3 cut(s) 107, 714, 869
BfuI GTATCC 1 cut(s) 512
BisI GCNGC 9 cut(s) 286, 383, 711, 714, 717, 759, 872, 894, 897
BlsI GCNGC 9 cut(s) 287, 384, 712, 715, 718, 760, 873, 895, 898
Bme18I GGWCC 2 cut(s) 22, 637
BmeT110I CYCGRG 1 cut(s) 769
BmgT120I GGNCC 3 cut(s) 22, 637, 767
BmiI GGNNCC 1 cut(s) 639
BmsI GCATC 3 cut(s) 96, 334, 575
BpiI GAAGAC 1 cut(s) 315
Bpu10I CCTNAGC 1 cut(s) 336
Bpu14I TTCGAA 1 cut(s) 155
BsaBI GATNNNNATC 1 cut(s) 123
BsaHI GRCGYC 1 cut(s) 305
BsaJI CCNNGG 1 cut(s) 186
Bse21I CCTNAGG 2 cut(s) 18, 786
Bse3DI GCAATG 1 cut(s) 835
Bse8I GATNNNNATC 1 cut(s) 123
BseDI CCNNGG 1 cut(s) 186
BseGI GGATG 2 cut(s) 84, 230
BseJI GATNNNNATC 1 cut(s) 123
BseMI GCAATG 1 cut(s) 835
BseMII CTCAG 2 cut(s) 327, 800
BseXI GCAGC 7 cut(s) 394, 700, 728, 745, 883, 883, 905
Bsh1236I CGCG 1 cut(s) 618
BshFI GGCC 2 cut(s) 669, 768
BsiHKAI GWGCWC 1 cut(s) 776
BsiHKCI CYCGRG 1 cut(s) 769
BsiWI CGTACG 1 cut(s) 630
BsnI GGCC 2 cut(s) 669, 768
BsoBI CYCGRG 1 cut(s) 769
Bsp119I TTCGAA 1 cut(s) 155
Bsp1286I GDGCHC 1 cut(s) 776
Bsp143I GATC 7 cut(s) 7, 161, 259, 502, 819, 842, 901
BspACI CCGC 6 cut(s) 273, 279, 285, 288, 603, 710
BspANI GGCC 2 cut(s) 669, 768
BspCNI CTCAG 2 cut(s) 328, 799
BspFNI CGCG 1 cut(s) 618
BspHI TCATGA 3 cut(s) 10, 58, 483
BspLI GGNNCC 1 cut(s) 639
BspMAI CTGCAG 3 cut(s) 111, 718, 873
BspPI GGATC 2 cut(s) 169, 850
BspT104I TTCGAA 1 cut(s) 155
BsrDI GCAATG 1 cut(s) 835
BssECI CCNNGG 1 cut(s) 186
BssMI GATC 7 cut(s) 7, 161, 259, 502, 819, 842, 901
BssNI GRCGYC 1 cut(s) 305
Bst4CI ACNGT 1 cut(s) 359
Bst6I CTCTTC 1 cut(s) 319
BstACI GRCGYC 1 cut(s) 305
BstBI TTCGAA 1 cut(s) 155
BstC8I GCNNGC 1 cut(s) 671
BstDEI CTNAG 5 cut(s) 18, 336, 417, 698, 786
BstDSI CCRYGG 1 cut(s) 186
BstF5I GGATG 2 cut(s) 84, 230
BstFNI CGCG 1 cut(s) 618
BstKTI GATC 7 cut(s) 10, 164, 262, 505, 822, 845, 904
BstMBI GATC 7 cut(s) 7, 161, 259, 502, 819, 842, 901
BstMWI GCNNNNNNNGC 3 cut(s) 285, 466, 716
BstSFI CTRYAG 3 cut(s) 107, 714, 869
BstUI CGCG 1 cut(s) 618
BstV1I GCAGC 7 cut(s) 394, 700, 728, 745, 883, 883, 905
BstV2I GAAGAC 1 cut(s) 315
BstX2I RGATCY 1 cut(s) 161
BstYI RGATCY 1 cut(s) 161
Bsu36I CCTNAGG 2 cut(s) 18, 786
BsuI GTATCC 1 cut(s) 512
BsuRI GGCC 2 cut(s) 669, 768
BtgI CCRYGG 1 cut(s) 186
BtsCI GGATG 2 cut(s) 84, 230
BtsIMutI CAGTG 1 cut(s) 324
Cac8I GCNNGC 1 cut(s) 671
CaiI CAGNNNCTG 1 cut(s) 899
CciI TCATGA 3 cut(s) 10, 58, 483
Cfr13I GGNCC 3 cut(s) 22, 637, 767
CseI GACGC 1 cut(s) 313
Csp6I GTAC 1 cut(s) 631
CspCI CAANNNNNGTGG 2 cut(s) 175, 210
CviAII CATG 7 cut(s) 11, 35, 59, 136, 484, 817, 890
CviQI GTAC 1 cut(s) 631
DdeI CTNAG 5 cut(s) 18, 336, 417, 698, 786
DpnI GATC 7 cut(s) 9, 163, 261, 504, 821, 844, 903
DpnII GATC 7 cut(s) 7, 161, 259, 502, 819, 842, 901
DraIII CACNNNGTG 1 cut(s) 150
Eam1104I CTCTTC 1 cut(s) 319
EarI CTCTTC 1 cut(s) 319
Ecl136II GAGCTC 1 cut(s) 774
Eco24I GRGCYC 1 cut(s) 776
Eco47I GGWCC 2 cut(s) 22, 637
Eco53kI GAGCTC 1 cut(s) 774
Eco57I CTGAAG 3 cut(s) 237, 300, 315
Eco81I CCTNAGG 2 cut(s) 18, 786
Eco88I CYCGRG 1 cut(s) 769
EcoICRI GAGCTC 1 cut(s) 774
EcoO109I RGGNCCY 2 cut(s) 22, 637
EcoT38I GRGCYC 1 cut(s) 776
FaeI CATG 7 cut(s) 14, 38, 62, 139, 487, 820, 893
FatI CATG 7 cut(s) 10, 34, 58, 135, 483, 816, 889
FauI CCCGC 1 cut(s) 596
FbaI TGATCA 5 cut(s) 7, 259, 502, 819, 901
Fnu4HI GCNGC 9 cut(s) 286, 383, 711, 714, 717, 759, 872, 894, 897
FokI GGATG 2 cut(s) 91, 217
FriOI GRGCYC 1 cut(s) 776
Fsp4HI GCNGC 9 cut(s) 286, 383, 711, 714, 717, 759, 872, 894, 897
FspBI CTAG 2 cut(s) 266, 509
GluI GCNGC 9 cut(s) 286, 383, 711, 714, 717, 759, 872, 894, 897
HaeIII GGCC 2 cut(s) 669, 768
HgaI GACGC 1 cut(s) 313
Hin1I GRCGYC 1 cut(s) 305
Hin1II CATG 7 cut(s) 14, 38, 62, 139, 487, 820, 893
HphI GGTGA 1 cut(s) 302
Hpy166II GTNNAC 1 cut(s) 169
Hpy188I TCNGA 3 cut(s) 7, 217, 400
Hpy188III TCNNGA 7 cut(s) 11, 59, 484, 509, 610, 788, 849
Hpy8I GTNNAC 1 cut(s) 169
HpyAV CCTTC 7 cut(s) 138, 233, 439, 490, 659, 741, 942
HpyCH4III ACNGT 1 cut(s) 359
HpyCH4IV ACGT 1 cut(s) 659
HpyCH4V TGCA 6 cut(s) 109, 588, 716, 871, 893, 919
HpyF10VI GCNNNNNNNGC 3 cut(s) 285, 466, 716
HpyF3I CTNAG 5 cut(s) 18, 336, 417, 698, 786
HpySE526I ACGT 1 cut(s) 659
Hsp92I GRCGYC 1 cut(s) 305
Hsp92II CATG 7 cut(s) 14, 38, 62, 139, 487, 820, 893
Ksp22I TGATCA 5 cut(s) 7, 259, 502, 819, 901
Kzo9I GATC 7 cut(s) 7, 161, 259, 502, 819, 842, 901
Lsp1109I GCAGC 7 cut(s) 394, 700, 728, 745, 883, 883, 905
LweI GCATC 3 cut(s) 96, 334, 575
MaeI CTAG 2 cut(s) 266, 509
MaeII ACGT 1 cut(s) 659
MaeIII GTNAC 1 cut(s) 846
MalI GATC 7 cut(s) 9, 163, 261, 504, 821, 844, 903
MboI GATC 7 cut(s) 7, 161, 259, 502, 819, 842, 901
MboII GAAGA 8 cut(s) 198, 306, 315, 318, 321, 413, 524, 857
MflI RGATCY 1 cut(s) 161
MhlI GDGCHC 1 cut(s) 776
MluI ACGCGT 1 cut(s) 616
MseI TTAA 1 cut(s) 834
MslI CAYNNNNRTG 2 cut(s) 69, 598
MspA1I CMGCKG 2 cut(s) 335, 896
MvnI CGCG 1 cut(s) 618
MwoI GCNNNNNNNGC 3 cut(s) 285, 466, 716
NdeII GATC 7 cut(s) 7, 161, 259, 502, 819, 842, 901
NlaIII CATG 7 cut(s) 14, 38, 62, 139, 487, 820, 893
NlaIV GGNNCC 1 cut(s) 639
NmuCI GTSAC 1 cut(s) 846
NspV TTCGAA 1 cut(s) 155
PagI TCATGA 3 cut(s) 10, 58, 483
Pfl23II CGTACG 1 cut(s) 630
PkrI GCNGC 9 cut(s) 287, 384, 712, 715, 718, 760, 873, 895, 898
PpuMI RGGWCCY 2 cut(s) 22, 637
Psp124BI GAGCTC 1 cut(s) 776
Psp5II RGGWCCY 2 cut(s) 22, 637
PspLI CGTACG 1 cut(s) 630
PspN4I GGNNCC 1 cut(s) 639
PspPI GGNCC 3 cut(s) 22, 637, 767
PspPPI RGGWCCY 2 cut(s) 22, 637
PstI CTGCAG 3 cut(s) 111, 718, 873
PstNI CAGNNNCTG 1 cut(s) 899
PsuI RGATCY 1 cut(s) 161
PvuII CAGCTG 2 cut(s) 335, 896
RsaI GTAC 1 cut(s) 632
RsaNI GTAC 1 cut(s) 631
RseI CAYNNNNRTG 2 cut(s) 69, 598
SacI GAGCTC 1 cut(s) 776
SaqAI TTAA 1 cut(s) 834
SatI GCNGC 9 cut(s) 286, 383, 711, 714, 717, 759, 872, 894, 897
Sau3AI GATC 7 cut(s) 7, 161, 259, 502, 819, 842, 901
Sau96I GGNCC 3 cut(s) 22, 637, 767
SduI GDGCHC 1 cut(s) 776
SfaNI GCATC 3 cut(s) 96, 334, 575
SfcI CTRYAG 3 cut(s) 107, 714, 869
SfuI TTCGAA 1 cut(s) 155
SinI GGWCC 2 cut(s) 22, 637
SmiMI CAYNNNNRTG 2 cut(s) 69, 598
SsiI CCGC 6 cut(s) 273, 279, 285, 288, 603, 710
SspMI CTAG 2 cut(s) 266, 509
SstI GAGCTC 1 cut(s) 776
TaaI ACNGT 1 cut(s) 359
TaiI ACGT 1 cut(s) 662
TaqI TCGA 3 cut(s) 155, 749, 935
TauI GCSGC 2 cut(s) 288, 713
Tru1I TTAA 1 cut(s) 834
Tru9I TTAA 1 cut(s) 834
TscAI CASTG 1 cut(s) 324
TseFI GTSAC 1 cut(s) 846
TseI GCWGC 7 cut(s) 382, 713, 716, 758, 871, 893, 896
Tsp45I GTSAC 1 cut(s) 846
TspDTI ATGAA 8 cut(s) 27, 66, 75, 120, 152, 219, 472, 678
TspGWI ACGGA 1 cut(s) 223
TspRI CASTG 1 cut(s) 324
VpaK11BI GGWCC 2 cut(s) 22, 637
XbaI TCTAGA 1 cut(s) 508
XspI CTAG 2 cut(s) 266, 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.