AT5G46650

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0016563
Physical Location & Seq
Forward (+)
18930246 .. 18931312
1067 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G46650.1

Sequence Viewer

Length: 870 bp
ATGCCGATAGCGAAACCGATCAACCAAAACACTACTGTTCCATATCCACCACAACATTACAGTAAACCGCCTCTAGTCATAATCCTAACCGTGATCCTCCTCGTCGTCTTTTTCATCGGTTTTTTCGCTATCTATTTCTGTAAATGTTTCTACCACACTCTCACCGAAGCTTGGAACCACCATTACCACAACGGATTGCCCGAAAATCAAATCCAAGCACAACAAGAACCCGTTCAACCACCGGTTAACCCTGGTCTAGAGCCACACATCATCCAATCCTACCCTTTGTTTCCATTCTCATCCGTCAAAGATCTCAGGGAAGACAAATACGGTCTCGAATGCGCGATTTGTCTACTTGAATTCGAAGAAGAACACATCCTCCTACGACTCTTGACAACTTGTTACCATGTTTTTCATCAAGAATGCATCGATCAATGGCTTGAATCTAACAAAACATGCCCTGTATGTCGTCGGAATTTAGATCCAAACGCGCCAGAAAACATCAAAGAGTTGATCATCGAAGTCATACAAGAAAACGCACATGAAAATCGCGATCAGGAACAAACGTCAACATCAAACGAGGTTATGTTGTCGAGACAAAGTAGTGGTAACAACGAGAGAAAGATTGAGACGTTACCGGATAAATTCTCGAGATCGAAAACGACAGGTCATTCAATTGTGAGGAATAAACCGGAGGAAGAAGATCGGTATACTTTGAGGTTACCGGATCATGTTAAGATTAAGGTTACGAGAAGACACAACAATAATCAAACAGAGAGTTGTATTTCATTTGGTGAGCTTGTTAGAAACAGAGAAGGCCGGTTTGGTGAAGTTTCTGGTCAATCACTTGTACCGGAATCAGGAAGTTAA

Protein Analysis

289

Amino Acids

33.43

Weight (kDa)

5.91

Isoelectric Point (pI)

73.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 113 - 157 1.9e-14 Ring finger domain
zf-C3HC4_2 PF13923 113 - 156 5.3e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 113 - 158 1.6e-07 zinc-RING finger domain
zf-RING_11 PF17123 113 - 143 9.5e-07 RING-like zinc finger
zf-C3HC4 PF00097 114 - 156 1.4e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 114 - 154 2e-07 RING-type zinc-finger
zf-rbx1 PF12678 124 - 157 3.6e-09 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016563)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17920 AT5G46650
fragaria_vesca FvH4_5g22380
malus_domestica MD06G1036300.v1.1
prunus_persica Prupe.5G033100_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0210121
rosa_laevigata RLG00000003063
rosa_multiflora Rmu_sc0025548.1_g000001
rosa_rugosa Rorug07G0118700
rosa_samantha Rh7AG252200 Rh7BG247000 Rh7CG269200 Rh7DG259500
rosa_wichuraiana Rw7G021430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 352, 710
AccII CGCG 3 cut(s) 344, 491, 552
AciI CCGC 1 cut(s) 68
AclWI GGATC 3 cut(s) 88, 476, 735
AcsI RAATTY 3 cut(s) 359, 475, 644
AfaI GTAC 1 cut(s) 852
AfiI CCNNNNNNNGG 2 cut(s) 171, 860
AgeI ACCGGT 1 cut(s) 241
AgsI TTSAA 4 cut(s) 236, 359, 443, 675
AjnI CCWGG 1 cut(s) 250
AjuI GAANNNNNNNTTGG 2 cut(s) 807, 839
AloI GAACNNNNNNTCC 2 cut(s) 363, 395
AluBI AGCT 2 cut(s) 170, 799
AluI AGCT 2 cut(s) 170, 799
Alw26I GTCTC 3 cut(s) 338, 589, 623
AlwI GGATC 3 cut(s) 88, 476, 735
Ama87I CYCGRG 1 cut(s) 649
AoxI GGCC 1 cut(s) 817
ApoI RAATTY 3 cut(s) 359, 475, 644
AsiGI ACCGGT 1 cut(s) 241
Asp700I GAANNNNTTC 1 cut(s) 231
AspLEI GCGC 2 cut(s) 344, 493
AsuHPI GGTGA 3 cut(s) 154, 806, 839
AsuII TTCGAA 1 cut(s) 363
AvaI CYCGRG 1 cut(s) 649
BbsI GAAGAC 2 cut(s) 327, 760
BciT130I CCWGG 1 cut(s) 252
BclI TGATCA 1 cut(s) 513
BcoDI GTCTC 3 cut(s) 338, 589, 623
BfaI CTAG 2 cut(s) 74, 257
BglII AGATCT 1 cut(s) 310
Bme1390I CCNGG 1 cut(s) 252
BmeT110I CYCGRG 1 cut(s) 649
BmiI GGNNCC 1 cut(s) 176
BmrFI CCNGG 1 cut(s) 252
BmsI GCATC 1 cut(s) 435
BpiI GAAGAC 2 cut(s) 327, 760
Bpu14I TTCGAA 1 cut(s) 363
Bsa29I ATCGAT 1 cut(s) 429
BsaI GGTCTC 1 cut(s) 338
BsaJI CCNNGG 1 cut(s) 250
BsaWI WCCGGW 5 cut(s) 241, 637, 691, 724, 853
BsaXI ACNNNNNCTCC 2 cut(s) 363, 393
Bsc4I CCNNNNNNNGG 2 cut(s) 171, 860
Bse118I RCCGGY 2 cut(s) 241, 819
BseBI CCWGG 1 cut(s) 252
BseCI ATCGAT 1 cut(s) 429
BseDI CCNNGG 1 cut(s) 250
BseGI GGATG 3 cut(s) 270, 299, 375
BseLI CCNNNNNNNGG 2 cut(s) 171, 860
BseMII CTCAG 1 cut(s) 328
BseRI GAGGAG 1 cut(s) 89
Bsh1236I CGCG 3 cut(s) 344, 491, 552
BshFI GGCC 1 cut(s) 819
BshTI ACCGGT 1 cut(s) 241
BshVI ATCGAT 1 cut(s) 429
BsiHKCI CYCGRG 1 cut(s) 649
BsiSI CCGG 6 cut(s) 242, 638, 692, 725, 820, 854
BslI CCNNNNNNNGG 2 cut(s) 171, 860
BsmAI GTCTC 3 cut(s) 338, 589, 623
BsmBI CGTCTC 1 cut(s) 623
BsmI GAATGC 2 cut(s) 344, 428
BsnI GGCC 1 cut(s) 819
Bso31I GGTCTC 1 cut(s) 338
BsoBI CYCGRG 1 cut(s) 649
Bsp119I TTCGAA 1 cut(s) 363
Bsp68I TCGCGA 1 cut(s) 552
BspACI CCGC 1 cut(s) 68
BspANI GGCC 1 cut(s) 819
BspCNI CTCAG 1 cut(s) 327
BspDI ATCGAT 1 cut(s) 429
BspFNI CGCG 3 cut(s) 344, 491, 552
BspLI GGNNCC 1 cut(s) 176
BspPI GGATC 3 cut(s) 88, 476, 735
BspT104I TTCGAA 1 cut(s) 363
BspTNI GGTCTC 1 cut(s) 338
BsrFI RCCGGY 2 cut(s) 241, 819
BssAI RCCGGY 2 cut(s) 241, 819
BssECI CCNNGG 1 cut(s) 250
BssNAI GTATAC 1 cut(s) 711
Bst1107I GTATAC 1 cut(s) 711
Bst2UI CCWGG 1 cut(s) 252
Bst4CI ACNGT 4 cut(s) 37, 62, 91, 332
BstBI TTCGAA 1 cut(s) 363
BstDEI CTNAG 1 cut(s) 314
BstEII GGTNACC 1 cut(s) 720
BstF5I GGATG 3 cut(s) 270, 299, 375
BstFNI CGCG 3 cut(s) 344, 491, 552
BstHHI GCGC 2 cut(s) 344, 493
BstMAI GTCTC 3 cut(s) 338, 589, 623
BstNI CCWGG 1 cut(s) 252
BstNSI RCATGY 1 cut(s) 459
BstPI GGTNACC 1 cut(s) 720
BstSCI CCNGG 1 cut(s) 250
BstUI CGCG 3 cut(s) 344, 491, 552
BstV2I GAAGAC 2 cut(s) 327, 760
BstX2I RGATCY 2 cut(s) 310, 481
BstYI RGATCY 2 cut(s) 310, 481
BstZ17I GTATAC 1 cut(s) 711
Bsu15I ATCGAT 1 cut(s) 429
BsuRI GGCC 1 cut(s) 819
BsuTUI ATCGAT 1 cut(s) 429
BtsCI GGATG 3 cut(s) 270, 299, 375
BtuMI TCGCGA 1 cut(s) 552
CfoI GCGC 2 cut(s) 344, 493
Cfr10I RCCGGY 2 cut(s) 241, 819
ClaI ATCGAT 1 cut(s) 429
Csp6I GTAC 1 cut(s) 851
CspAI ACCGGT 1 cut(s) 241
CviAII CATG 4 cut(s) 407, 456, 542, 731
CviJI RGCY 5 cut(s) 170, 262, 439, 799, 819
CviKI_1 RGCY 5 cut(s) 170, 262, 439, 799, 819
CviQI GTAC 1 cut(s) 851
DdeI CTNAG 1 cut(s) 314
Eco31I GGTCTC 1 cut(s) 338
Eco88I CYCGRG 1 cut(s) 649
Eco91I GGTNACC 1 cut(s) 720
EcoO65I GGTNACC 1 cut(s) 720
EcoRI GAATTC 1 cut(s) 359
EcoRII CCWGG 1 cut(s) 250
EcoT22I ATGCAT 1 cut(s) 428
Esp3I CGTCTC 1 cut(s) 623
FaeI CATG 4 cut(s) 410, 459, 545, 734
FatI CATG 4 cut(s) 406, 455, 541, 730
FbaI TGATCA 1 cut(s) 513
FblI GTMKAC 2 cut(s) 352, 710
FokI GGATG 3 cut(s) 257, 286, 362
FspBI CTAG 2 cut(s) 74, 257
GlaI GCGC 2 cut(s) 343, 492
HaeIII GGCC 1 cut(s) 819
HapII CCGG 6 cut(s) 242, 638, 692, 725, 820, 854
HhaI GCGC 2 cut(s) 344, 493
Hin1II CATG 4 cut(s) 410, 459, 545, 734
Hin6I GCGC 2 cut(s) 342, 491
HinP1I GCGC 2 cut(s) 342, 491
HincII GTYRAC 2 cut(s) 247, 570
HindII GTYRAC 2 cut(s) 247, 570
HindIII AAGCTT 1 cut(s) 168
HinfI GANTC 3 cut(s) 387, 443, 857
HpaI GTTAAC 1 cut(s) 247
HpaII CCGG 6 cut(s) 242, 638, 692, 725, 820, 854
HphI GGTGA 3 cut(s) 154, 806, 839
Hpy166II GTNNAC 5 cut(s) 65, 247, 353, 570, 711
Hpy188I TCNGA 1 cut(s) 474
Hpy8I GTNNAC 5 cut(s) 65, 247, 353, 570, 711
Hpy99I CGWCG 2 cut(s) 107, 474
HpyAV CCTTC 1 cut(s) 809
HpyCH4III ACNGT 4 cut(s) 37, 62, 91, 332
HpyCH4IV ACGT 2 cut(s) 566, 632
HpyCH4V TGCA 1 cut(s) 426
HpyF3I CTNAG 1 cut(s) 314
HpySE526I ACGT 2 cut(s) 566, 632
Hsp92II CATG 4 cut(s) 410, 459, 545, 734
HspAI GCGC 2 cut(s) 342, 491
Ksp22I TGATCA 1 cut(s) 513
KspAI GTTAAC 1 cut(s) 247
LweI GCATC 1 cut(s) 435
MaeI CTAG 2 cut(s) 74, 257
MaeII ACGT 2 cut(s) 566, 632
MaeIII GTNAC 5 cut(s) 401, 608, 633, 720, 745
MboII GAAGA 6 cut(s) 332, 377, 380, 710, 713, 765
MfeI CAATTG 1 cut(s) 675
MflI RGATCY 2 cut(s) 310, 481
MluCI AATT 4 cut(s) 359, 475, 644, 675
MlyI GAGTC 1 cut(s) 381
MmeI TCCRAC 1 cut(s) 452
MnlI CCTC 8 cut(s) 81, 107, 110, 389, 574, 675, 688, 711
Mph1103I ATGCAT 1 cut(s) 428
MroXI GAANNNNTTC 1 cut(s) 231
MseI TTAA 4 cut(s) 246, 735, 741, 868
MspI CCGG 6 cut(s) 242, 638, 692, 725, 820, 854
MspR9I CCNGG 1 cut(s) 252
MunI CAATTG 1 cut(s) 675
Mva1269I GAATGC 2 cut(s) 344, 428
MvaI CCWGG 1 cut(s) 252
MvnI CGCG 3 cut(s) 344, 491, 552
NlaIII CATG 4 cut(s) 410, 459, 545, 734
NlaIV GGNNCC 1 cut(s) 176
NruI TCGCGA 1 cut(s) 552
NsiI ATGCAT 1 cut(s) 428
NspI RCATGY 1 cut(s) 459
NspV TTCGAA 1 cut(s) 363
PaeR7I CTCGAG 1 cut(s) 649
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PctI GAATGC 2 cut(s) 344, 428
PdmI GAANNNNTTC 1 cut(s) 231
PfeI GAWTC 2 cut(s) 443, 857
PinAI ACCGGT 1 cut(s) 241
PleI GAGTC 1 cut(s) 381
PpsI GAGTC 1 cut(s) 381
Psp6I CCWGG 1 cut(s) 250
PspEI GGTNACC 1 cut(s) 720
PspGI CCWGG 1 cut(s) 250
PspN4I GGNNCC 1 cut(s) 176
PsrI GAACNNNNNNTAC 2 cut(s) 167, 199
PsuI RGATCY 2 cut(s) 310, 481
RruI TCGCGA 1 cut(s) 552
RsaI GTAC 1 cut(s) 852
RsaNI GTAC 1 cut(s) 851
SaqAI TTAA 4 cut(s) 246, 735, 741, 868
SchI GAGTC 1 cut(s) 381
ScrFI CCNGG 1 cut(s) 252
SetI ASST 8 cut(s) 172, 569, 585, 635, 670, 722, 747, 801
SfaNI GCATC 1 cut(s) 435
Sfr274I CTCGAG 1 cut(s) 649
SfuI TTCGAA 1 cut(s) 363
SlaI CTCGAG 1 cut(s) 649
SmlI CTYRAG 1 cut(s) 649
SmoI CTYRAG 1 cut(s) 649
Sse9I AATT 4 cut(s) 359, 475, 644, 675
SsiI CCGC 1 cut(s) 68
SspMI CTAG 2 cut(s) 74, 257
StyD4I CCNGG 1 cut(s) 250
TaaI ACNGT 4 cut(s) 37, 62, 91, 332
TaiI ACGT 2 cut(s) 569, 635
TaqI TCGA 7 cut(s) 336, 363, 429, 519, 593, 650, 656
TasI AATT 4 cut(s) 359, 475, 644, 675
TfiI GAWTC 2 cut(s) 443, 857
Tru1I TTAA 4 cut(s) 246, 735, 741, 868
Tru9I TTAA 4 cut(s) 246, 735, 741, 868
TspDTI ATGAA 4 cut(s) 103, 404, 558, 777
TspGWI ACGGA 2 cut(s) 207, 292
XapI RAATTY 3 cut(s) 359, 475, 644
XbaI TCTAGA 1 cut(s) 256
XceI RCATGY 1 cut(s) 459
XhoI CTCGAG 1 cut(s) 649
XmiI GTMKAC 2 cut(s) 352, 710
XmnI GAANNNNTTC 1 cut(s) 231
XspI CTAG 2 cut(s) 74, 257
Zsp2I ATGCAT 1 cut(s) 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.