Rh7BG247000

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0016563
Physical Location & Seq
Reverse (-)
22074808 .. 22076191
1384 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG247000.1

Sequence Viewer

Length: 924 bp
ATGCTGGGAATGTCAACGAATTCCAATAGTTACCCTCCACCGGAAAATATCAGTTCCTCTCCATCGATCACTGTCCTTTTGACCGTCCTCCTCCTCGTCTTCTTCTTTCTTGGTTTCTTTTCTATTTACTTTTGCAAGTGTTTCGTGGAAAACGTCCTCACGTCTTTTCAGCGCACACCCTCCGGCGACCTTGTGGGCGTACCTGTTCCTCCCAAAGAAGGCCTCGACCCCTCTCTAATACAATCATTTCCTACCTTCATCTACGCAAGTGTCAAAGATTTTCGCATAGAGAAGTACGGTCTAGAATGTGCAATTTGCTTGGTGGAGTTTGAGGATGATAGCATGCTACGTCTTATAACAGCTTGTTGCCATGTCTTCCACCAAGAATGCATCGACTTGTGGCTCGAGTCTCACAAAACATGTCCCTTTTGCCGTGGAAACCTTGAATTGTCGCCAACTATAATGGACAAGTCTCCGGTGCTTGCTCATGAGAATGCCATGCACGAAATTAATGAAGGTGAAGAGTCCATATACGGTCAAGATGTTATCCGAATTGACATTAAAGACGAAGACGAAGAAGACCGAGAAGGAAATCGTAGTGATCATCAACAAAATTTGGCTGCTAATGTAAATTCTAACGTGGAAGATCAACAAATTGATGGACAAAATGAAGAGAACGAAACGGAAAGATTTTCGAGGTCACACTCGACAGGACACTCCATAGTTAGGCCTAGACAAGAGGAGGATAGGTATACACTCAGATTACCAGAGCATGTGAAGATAAAACTTCTTAGAGGCCACAATTGGACAGGAAGTTGTACTACATTTGGGGAGTTTTCACGACAAAGAGGCAGTTGTAAAGGTGGCTTCGGAGAGGTCTCAGAATCTAGCTCTGCTGGTGGAGAGTTTAGTAGAAAACTGTAA

Protein Analysis

307

Amino Acids

34.65

Weight (kDa)

4.92

Isoelectric Point (pI)

62.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 102 - 145 1.6e-13 Ring finger domain
zf-C3HC4_2 PF13923 102 - 144 1.8e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 102 - 131 6.3e-08 RING-like zinc finger
zf-C3HC4 PF00097 103 - 144 9e-06 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 106 - 145 1.3e-06 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016563)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17920 AT5G46650
fragaria_vesca FvH4_5g22380
malus_domestica MD06G1036300.v1.1
prunus_persica Prupe.5G033100_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0210121
rosa_laevigata RLG00000003063
rosa_multiflora Rmu_sc0025548.1_g000001
rosa_rugosa Rorug07G0118700
rosa_samantha Rh7AG252200 Rh7BG247000 Rh7CG269200 Rh7DG259500
rosa_wichuraiana Rw7G021430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 356
AccI GTMKAC 1 cut(s) 752
AcsI RAATTY 3 cut(s) 19, 613, 631
AfaI GTAC 3 cut(s) 201, 296, 820
AfiI CCNNNNNNNGG 3 cut(s) 40, 218, 726
AflIII ACRYGT 1 cut(s) 419
AgsI TTSAA 1 cut(s) 446
AjiI CACGTC 1 cut(s) 162
AluBI AGCT 2 cut(s) 362, 891
AluI AGCT 2 cut(s) 362, 891
Alw26I GTCTC 3 cut(s) 414, 477, 883
Ama87I CYCGRG 1 cut(s) 404
AoxI GGCC 3 cut(s) 220, 728, 796
ApeKI GCWGC 1 cut(s) 620
ApoI RAATTY 3 cut(s) 19, 613, 631
AseI ATTAAT 1 cut(s) 510
AspLEI GCGC 1 cut(s) 174
AsuHPI GGTGA 1 cut(s) 530
AvaI CYCGRG 1 cut(s) 404
BarI GAAGNNNNNNTAC 2 cut(s) 805, 837
BbsI GAAGAC 4 cut(s) 91, 367, 576, 585
BbvI GCAGC 1 cut(s) 607
BccI CCATC 2 cut(s) 70, 653
BceAI ACGGC 1 cut(s) 417
BcgI CGANNNNNNTGC 2 cut(s) 124, 158
BclI TGATCA 1 cut(s) 601
BcoDI GTCTC 3 cut(s) 414, 477, 883
BfaI CTAG 3 cut(s) 302, 732, 888
BisI GCNGC 1 cut(s) 621
BlsI GCNGC 1 cut(s) 622
BmeT110I CYCGRG 1 cut(s) 404
BmgBI CACGTC 1 cut(s) 162
BmsI GCATC 1 cut(s) 399
BpiI GAAGAC 4 cut(s) 91, 367, 576, 585
Bsa29I ATCGAT 1 cut(s) 65
BsaI GGTCTC 1 cut(s) 883
BsaJI CCNNGG 1 cut(s) 433
BsaWI WCCGGW 2 cut(s) 40, 475
BsaXI ACNNNNNCTCC 2 cut(s) 734, 764
Bsc4I CCNNNNNNNGG 3 cut(s) 40, 218, 726
BseCI ATCGAT 1 cut(s) 65
BseDI CCNNGG 1 cut(s) 433
BseGI GGATG 1 cut(s) 340
BseLI CCNNNNNNNGG 3 cut(s) 40, 218, 726
BseMII CTCAG 2 cut(s) 772, 894
BseRI GAGGAG 3 cut(s) 80, 83, 755
BseXI GCAGC 1 cut(s) 607
BseYI CCCAGC 1 cut(s) 4
BshFI GGCC 3 cut(s) 222, 730, 798
BshVI ATCGAT 1 cut(s) 65
BsiHKCI CYCGRG 1 cut(s) 404
BsiSI CCGG 3 cut(s) 41, 183, 476
BslFI GGGAC 1 cut(s) 408
BslI CCNNNNNNNGG 3 cut(s) 40, 218, 726
BsmAI GTCTC 3 cut(s) 414, 477, 883
BsmFI GGGAC 1 cut(s) 408
BsmI GAATGC 2 cut(s) 392, 499
BsnI GGCC 3 cut(s) 222, 730, 798
Bso31I GGTCTC 1 cut(s) 883
BsoBI CYCGRG 1 cut(s) 404
Bsp143I GATC 3 cut(s) 66, 601, 646
BspANI GGCC 3 cut(s) 222, 730, 798
BspCNI CTCAG 2 cut(s) 771, 893
BspDI ATCGAT 1 cut(s) 65
BspHI TCATGA 1 cut(s) 487
BspTNI GGTCTC 1 cut(s) 883
BssECI CCNNGG 1 cut(s) 433
BssMI GATC 3 cut(s) 66, 601, 646
BssNAI GTATAC 1 cut(s) 753
Bst1107I GTATAC 1 cut(s) 753
Bst4CI ACNGT 5 cut(s) 73, 85, 299, 536, 921
Bst6I CTCTTC 2 cut(s) 516, 666
BstC8I GCNNGC 2 cut(s) 344, 483
BstDEI CTNAG 3 cut(s) 758, 791, 880
BstDSI CCRYGG 1 cut(s) 433
BstF5I GGATG 1 cut(s) 340
BstHHI GCGC 1 cut(s) 174
BstKTI GATC 3 cut(s) 69, 604, 649
BstMAI GTCTC 3 cut(s) 414, 477, 883
BstMBI GATC 3 cut(s) 66, 601, 646
BstNSI RCATGY 3 cut(s) 346, 423, 776
BstV1I GCAGC 1 cut(s) 607
BstV2I GAAGAC 4 cut(s) 91, 367, 576, 585
BstZ17I GTATAC 1 cut(s) 753
Bsu15I ATCGAT 1 cut(s) 65
BsuRI GGCC 3 cut(s) 222, 730, 798
BsuTUI ATCGAT 1 cut(s) 65
BtgI CCRYGG 1 cut(s) 433
BtrI CACGTC 1 cut(s) 162
BtsCI GGATG 1 cut(s) 340
BtsIMutI CAGTG 1 cut(s) 69
Cac8I GCNNGC 2 cut(s) 344, 483
CciI TCATGA 1 cut(s) 487
CfoI GCGC 1 cut(s) 174
ClaI ATCGAT 1 cut(s) 65
Csp6I GTAC 3 cut(s) 200, 295, 819
CviAII CATG 6 cut(s) 343, 371, 420, 488, 499, 773
CviJI RGCY 8 cut(s) 222, 362, 403, 620, 730, 798, 867, 891
CviKI_1 RGCY 8 cut(s) 222, 362, 403, 620, 730, 798, 867, 891
CviQI GTAC 3 cut(s) 200, 295, 819
DdeI CTNAG 3 cut(s) 758, 791, 880
DpnI GATC 3 cut(s) 68, 603, 648
DpnII GATC 3 cut(s) 66, 601, 646
Eam1104I CTCTTC 2 cut(s) 516, 666
EarI CTCTTC 2 cut(s) 516, 666
Eco147I AGGCCT 2 cut(s) 222, 730
Eco31I GGTCTC 1 cut(s) 883
Eco88I CYCGRG 1 cut(s) 404
EcoRI GAATTC 1 cut(s) 19
EcoT22I ATGCAT 1 cut(s) 392
FaeI CATG 6 cut(s) 346, 374, 423, 491, 502, 776
FaqI GGGAC 1 cut(s) 408
FatI CATG 6 cut(s) 342, 370, 419, 487, 498, 772
FbaI TGATCA 1 cut(s) 601
FblI GTMKAC 1 cut(s) 752
Fnu4HI GCNGC 1 cut(s) 621
FokI GGATG 1 cut(s) 347
Fsp4HI GCNGC 1 cut(s) 621
FspBI CTAG 3 cut(s) 302, 732, 888
GlaI GCGC 1 cut(s) 173
GluI GCNGC 1 cut(s) 621
GsaI CCCAGC 1 cut(s) 8
HaeIII GGCC 3 cut(s) 222, 730, 798
HapII CCGG 3 cut(s) 41, 183, 476
HhaI GCGC 1 cut(s) 174
Hin1II CATG 6 cut(s) 346, 374, 423, 491, 502, 776
Hin6I GCGC 1 cut(s) 172
HinP1I GCGC 1 cut(s) 172
HincII GTYRAC 1 cut(s) 15
HindII GTYRAC 1 cut(s) 15
HinfI GANTC 3 cut(s) 407, 524, 884
HpaII CCGG 3 cut(s) 41, 183, 476
HphI GGTGA 1 cut(s) 530
Hpy166II GTNNAC 2 cut(s) 15, 753
Hpy188I TCNGA 4 cut(s) 551, 761, 872, 883
Hpy188III TCNNGA 4 cut(s) 302, 488, 539, 840
Hpy8I GTNNAC 2 cut(s) 15, 753
HpyAV CCTTC 4 cut(s) 212, 265, 509, 581
HpyCH4III ACNGT 5 cut(s) 73, 85, 299, 536, 921
HpyCH4IV ACGT 4 cut(s) 153, 161, 349, 639
HpyCH4V TGCA 4 cut(s) 135, 311, 390, 502
HpyF3I CTNAG 3 cut(s) 758, 791, 880
HpySE526I ACGT 4 cut(s) 153, 161, 349, 639
Hsp92II CATG 6 cut(s) 346, 374, 423, 491, 502, 776
HspAI GCGC 1 cut(s) 172
Ksp22I TGATCA 1 cut(s) 601
Kzo9I GATC 3 cut(s) 66, 601, 646
LpnPI CCDG 8 cut(s) 54, 196, 216, 489, 696, 780, 795, 882
Lsp1109I GCAGC 1 cut(s) 607
LweI GCATC 1 cut(s) 399
MaeI CTAG 3 cut(s) 302, 732, 888
MaeII ACGT 4 cut(s) 153, 161, 349, 639
MaeIII GTNAC 2 cut(s) 29, 699
MalI GATC 3 cut(s) 68, 603, 648
MboI GATC 3 cut(s) 66, 601, 646
MfeI CAATTG 1 cut(s) 802
MluCI AATT 9 cut(s) 19, 312, 446, 507, 552, 613, 631, 654, 802
MlyI GAGTC 2 cut(s) 416, 533
Mph1103I ATGCAT 1 cut(s) 392
MseI TTAA 2 cut(s) 510, 561
MslI CAYNNNNRTG 1 cut(s) 492
MspI CCGG 3 cut(s) 41, 183, 476
MunI CAATTG 1 cut(s) 802
Mva1269I GAATGC 2 cut(s) 392, 499
NdeII GATC 3 cut(s) 66, 601, 646
NlaIII CATG 6 cut(s) 346, 374, 423, 491, 502, 776
NmuCI GTSAC 1 cut(s) 699
NsiI ATGCAT 1 cut(s) 392
NspI RCATGY 3 cut(s) 346, 423, 776
PaeI GCATGC 1 cut(s) 346
PaeR7I CTCGAG 1 cut(s) 404
PagI TCATGA 1 cut(s) 487
PceI AGGCCT 2 cut(s) 222, 730
PciI ACATGT 1 cut(s) 419
PcsI WCGNNNNNNNCGW 1 cut(s) 150
PctI GAATGC 2 cut(s) 392, 499
PfeI GAWTC 1 cut(s) 884
PkrI GCNGC 1 cut(s) 622
PleI GAGTC 2 cut(s) 415, 532
PpsI GAGTC 2 cut(s) 415, 532
PscI ACATGT 1 cut(s) 419
PshBI ATTAAT 1 cut(s) 510
PsiI TTATAA 1 cut(s) 356
PspFI CCCAGC 1 cut(s) 4
PspXI VCTCGAGB 1 cut(s) 404
RsaI GTAC 3 cut(s) 201, 296, 820
RsaNI GTAC 3 cut(s) 200, 295, 819
RseI CAYNNNNRTG 1 cut(s) 492
SaqAI TTAA 2 cut(s) 510, 561
SatI GCNGC 1 cut(s) 621
Sau3AI GATC 3 cut(s) 66, 601, 646
SchI GAGTC 2 cut(s) 416, 533
SfaNI GCATC 1 cut(s) 399
Sfr274I CTCGAG 1 cut(s) 404
SlaI CTCGAG 1 cut(s) 404
SmiMI CAYNNNNRTG 1 cut(s) 492
SmlI CTYRAG 1 cut(s) 404
SmoI CTYRAG 1 cut(s) 404
SphI GCATGC 1 cut(s) 346
Sse9I AATT 9 cut(s) 19, 312, 446, 507, 552, 613, 631, 654, 802
SseBI AGGCCT 2 cut(s) 222, 730
SspMI CTAG 3 cut(s) 302, 732, 888
StuI AGGCCT 2 cut(s) 222, 730
TaaI ACNGT 5 cut(s) 73, 85, 299, 536, 921
TaiI ACGT 4 cut(s) 156, 164, 352, 642
TaqI TCGA 6 cut(s) 65, 225, 393, 405, 695, 707
TaqII GACCGA 1 cut(s) 597
TasI AATT 9 cut(s) 19, 312, 446, 507, 552, 613, 631, 654, 802
TatI WGTACW 1 cut(s) 818
TfiI GAWTC 1 cut(s) 884
Tru1I TTAA 2 cut(s) 510, 561
Tru9I TTAA 2 cut(s) 510, 561
TscAI CASTG 1 cut(s) 76
TseFI GTSAC 1 cut(s) 699
TseI GCWGC 1 cut(s) 620
Tsp45I GTSAC 1 cut(s) 699
TspDTI ATGAA 3 cut(s) 247, 528, 684
TspGWI ACGGA 1 cut(s) 698
TspRI CASTG 1 cut(s) 76
VspI ATTAAT 1 cut(s) 510
XapI RAATTY 3 cut(s) 19, 613, 631
XbaI TCTAGA 1 cut(s) 301
XceI RCATGY 3 cut(s) 346, 423, 776
XhoI CTCGAG 1 cut(s) 404
XmiI GTMKAC 1 cut(s) 752
XspI CTAG 3 cut(s) 302, 732, 888
Zsp2I ATGCAT 1 cut(s) 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.