RLG00000003063

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0016563
Physical Location & Seq
Forward (+)
43856118 .. 43857029
912 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003063

Sequence Viewer

Length: 912 bp
ATGTCAACGAATTCTAATAGTTACCCTCCACCGGAAAATATCAGTTCCTCTCCATCGATCACTGTCCTTTTGACCGTCCTTCTCCTCGTCTTCTTCTTTCTTGGTTTCTTTTCTATTTACTTTTGCAAGTGTTTCGTGGAAAACGTCCTCAGGTCTTTTCAGCGCACACCCTCCGGCGACCTTGTGGGCGTACCTGTTCCTCCCAAAGAAGGCCTCGACCTCTCTCTAATACAATCATTTCCCACCTTCATCTATGCAAGTGTCAAAGATTTTCGCATAGAGAAGTACGGTCTAGAATGCGCAATTTGCTTGGTGGAGTTTGAGGATGATAGCATGCTACGTCTTTTAACAGCTTGTTGCCATGTCTTCCACCAAGAATGCATCGACCTGTGGCTCGAGTCTCACAAAACATGTCCCTTTTGCCGTGGAAACCTTGAATTGTCGCCAACTATAATGGACAAGTCTCCGGTGCTTGCTCATGAGAATGCCATGCACGAAATTAATGAAGGTGAAGAGTCCATATACGGTCAAGATGCTATCCGAATTGACATTAAAGACGAAGGCGAAGAAGGCCGAGAAGGAAATCGTAGTGCTCATCAACAAAATTTGGCCACTAATGTAAATTCTAACGTGGAAGATCAACAAATTGATGGACAAAATGAAGAGAATGAAACGGAAAGATTTTCGAGGTCACACTCGACAGGACACTCCATAGTTAGGCCTAGACAAGAGGAGGATAGGTATACACTCAGATTACCAGAGCATGTGAAGATAAAACTTCTTAGAGGGCACAATTGGACAGGAAGTTGTACTACATTTGGGGAGTTTTCACGACAAAGAGGAAGTTGTAAAGGTGGATTTGGAGAGGTCTCAGAATCTAGCTCTGGTGGAGAGATTAGTAGCAAACTGTAA

Protein Analysis

304

Amino Acids

34.08

Weight (kDa)

5.07

Isoelectric Point (pI)

63.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 99 - 142 1.1e-13 Ring finger domain
zf-RING_11 PF17123 99 - 128 1.2e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 99 - 141 2.3e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 100 - 141 8.9e-06 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 102 - 142 1.1e-06 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016563)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17920 AT5G46650
fragaria_vesca FvH4_5g22380
malus_domestica MD06G1036300.v1.1
prunus_persica Prupe.5G033100_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0210121
rosa_laevigata RLG00000003063
rosa_multiflora Rmu_sc0025548.1_g000001
rosa_rugosa Rorug07G0118700
rosa_samantha Rh7AG252200 Rh7BG247000 Rh7CG269200 Rh7DG259500
rosa_wichuraiana Rw7G021430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 301
AccI GTMKAC 1 cut(s) 743
AcoI YGGCCR 1 cut(s) 609
AcsI RAATTY 3 cut(s) 10, 604, 622
AfaI GTAC 3 cut(s) 192, 287, 811
AfiI CCNNNNNNNGG 3 cut(s) 31, 209, 717
AflIII ACRYGT 1 cut(s) 410
AgsI TTSAA 1 cut(s) 437
AluBI AGCT 2 cut(s) 353, 882
AluI AGCT 2 cut(s) 353, 882
Alw21I GWGCWC 1 cut(s) 595
Alw26I GTCTC 3 cut(s) 405, 468, 874
Ama87I CYCGRG 1 cut(s) 395
AoxI GGCC 4 cut(s) 211, 571, 609, 719
ApoI RAATTY 3 cut(s) 10, 604, 622
AseI ATTAAT 1 cut(s) 501
AspLEI GCGC 2 cut(s) 165, 302
AsuHPI GGTGA 1 cut(s) 521
AvaI CYCGRG 1 cut(s) 395
AxyI CCTNAGG 1 cut(s) 149
BaeGI GKGCMC 1 cut(s) 792
BalI TGGCCA 1 cut(s) 611
BarI GAAGNNNNNNTAC 2 cut(s) 796, 828
BbsI GAAGAC 2 cut(s) 82, 358
Bbv12I GWGCWC 1 cut(s) 595
BccI CCATC 2 cut(s) 61, 644
BceAI ACGGC 1 cut(s) 408
BcgI CGANNNNNNTGC 2 cut(s) 115, 149
BcoDI GTCTC 3 cut(s) 405, 468, 874
BfaI CTAG 3 cut(s) 293, 723, 879
BmeT110I CYCGRG 1 cut(s) 395
BmsI GCATC 2 cut(s) 390, 523
BpiI GAAGAC 2 cut(s) 82, 358
Bsa29I ATCGAT 1 cut(s) 56
BsaI GGTCTC 1 cut(s) 874
BsaJI CCNNGG 1 cut(s) 424
BsaWI WCCGGW 2 cut(s) 31, 466
BsaXI ACNNNNNCTCC 2 cut(s) 725, 755
Bsc4I CCNNNNNNNGG 3 cut(s) 31, 209, 717
Bse21I CCTNAGG 1 cut(s) 149
BseCI ATCGAT 1 cut(s) 56
BseDI CCNNGG 1 cut(s) 424
BseGI GGATG 1 cut(s) 331
BseLI CCNNNNNNNGG 3 cut(s) 31, 209, 717
BseMII CTCAG 3 cut(s) 163, 763, 885
BseRI GAGGAG 2 cut(s) 74, 746
BseSI GKGCMC 1 cut(s) 792
BshFI GGCC 4 cut(s) 213, 573, 611, 721
BshVI ATCGAT 1 cut(s) 56
BsiHKAI GWGCWC 1 cut(s) 595
BsiHKCI CYCGRG 1 cut(s) 395
BsiSI CCGG 3 cut(s) 32, 174, 467
BslFI GGGAC 1 cut(s) 399
BslI CCNNNNNNNGG 3 cut(s) 31, 209, 717
BsmAI GTCTC 3 cut(s) 405, 468, 874
BsmFI GGGAC 1 cut(s) 399
BsmI GAATGC 3 cut(s) 302, 383, 490
BsnI GGCC 4 cut(s) 213, 573, 611, 721
Bso31I GGTCTC 1 cut(s) 874
BsoBI CYCGRG 1 cut(s) 395
Bsp1286I GDGCHC 2 cut(s) 595, 792
Bsp143I GATC 2 cut(s) 57, 637
BspANI GGCC 4 cut(s) 213, 573, 611, 721
BspCNI CTCAG 3 cut(s) 162, 762, 884
BspDI ATCGAT 1 cut(s) 56
BspHI TCATGA 1 cut(s) 478
BspTNI GGTCTC 1 cut(s) 874
BssECI CCNNGG 1 cut(s) 424
BssMI GATC 2 cut(s) 57, 637
BssNAI GTATAC 1 cut(s) 744
Bst1107I GTATAC 1 cut(s) 744
Bst4CI ACNGT 5 cut(s) 64, 76, 290, 527, 909
Bst6I CTCTTC 2 cut(s) 507, 657
BstC8I GCNNGC 2 cut(s) 335, 474
BstDEI CTNAG 4 cut(s) 149, 749, 782, 871
BstDSI CCRYGG 1 cut(s) 424
BstF5I GGATG 1 cut(s) 331
BstHHI GCGC 2 cut(s) 165, 302
BstKTI GATC 2 cut(s) 60, 640
BstMAI GTCTC 3 cut(s) 405, 468, 874
BstMBI GATC 2 cut(s) 57, 637
BstMWI GCNNNNNNNGC 2 cut(s) 306, 570
BstNSI RCATGY 3 cut(s) 337, 414, 767
BstSLI GKGCMC 1 cut(s) 792
BstV2I GAAGAC 2 cut(s) 82, 358
BstZ17I GTATAC 1 cut(s) 744
Bsu15I ATCGAT 1 cut(s) 56
Bsu36I CCTNAGG 1 cut(s) 149
BsuRI GGCC 4 cut(s) 213, 573, 611, 721
BsuTUI ATCGAT 1 cut(s) 56
BtgI CCRYGG 1 cut(s) 424
BtsCI GGATG 1 cut(s) 331
BtsIMutI CAGTG 1 cut(s) 60
Cac8I GCNNGC 2 cut(s) 335, 474
CciI TCATGA 1 cut(s) 478
CfoI GCGC 2 cut(s) 165, 302
ClaI ATCGAT 1 cut(s) 56
Csp6I GTAC 3 cut(s) 191, 286, 810
CviAII CATG 6 cut(s) 334, 362, 411, 479, 490, 764
CviJI RGCY 7 cut(s) 213, 353, 394, 573, 611, 721, 882
CviKI_1 RGCY 7 cut(s) 213, 353, 394, 573, 611, 721, 882
CviQI GTAC 3 cut(s) 191, 286, 810
DdeI CTNAG 4 cut(s) 149, 749, 782, 871
DpnI GATC 2 cut(s) 59, 639
DpnII GATC 2 cut(s) 57, 637
EaeI YGGCCR 1 cut(s) 609
Eam1104I CTCTTC 2 cut(s) 507, 657
EarI CTCTTC 2 cut(s) 507, 657
Eco147I AGGCCT 2 cut(s) 213, 721
Eco31I GGTCTC 1 cut(s) 874
Eco81I CCTNAGG 1 cut(s) 149
Eco88I CYCGRG 1 cut(s) 395
EcoRI GAATTC 1 cut(s) 10
EcoT22I ATGCAT 1 cut(s) 383
FaeI CATG 6 cut(s) 337, 365, 414, 482, 493, 767
FaqI GGGAC 1 cut(s) 399
FatI CATG 6 cut(s) 333, 361, 410, 478, 489, 763
FblI GTMKAC 1 cut(s) 743
FokI GGATG 1 cut(s) 338
FspBI CTAG 3 cut(s) 293, 723, 879
FspI TGCGCA 1 cut(s) 301
GlaI GCGC 2 cut(s) 164, 301
HaeIII GGCC 4 cut(s) 213, 573, 611, 721
HapII CCGG 3 cut(s) 32, 174, 467
HhaI GCGC 2 cut(s) 165, 302
Hin1II CATG 6 cut(s) 337, 365, 414, 482, 493, 767
Hin6I GCGC 2 cut(s) 163, 300
HinP1I GCGC 2 cut(s) 163, 300
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 3 cut(s) 398, 515, 875
HpaII CCGG 3 cut(s) 32, 174, 467
HphI GGTGA 1 cut(s) 521
Hpy166II GTNNAC 2 cut(s) 6, 744
Hpy188I TCNGA 3 cut(s) 542, 752, 874
Hpy188III TCNNGA 4 cut(s) 293, 479, 530, 831
Hpy8I GTNNAC 2 cut(s) 6, 744
HpyAV CCTTC 7 cut(s) 89, 203, 256, 500, 554, 563, 572
HpyCH4III ACNGT 5 cut(s) 64, 76, 290, 527, 909
HpyCH4IV ACGT 3 cut(s) 144, 340, 630
HpyCH4V TGCA 4 cut(s) 126, 257, 381, 493
HpyF10VI GCNNNNNNNGC 2 cut(s) 306, 570
HpyF3I CTNAG 4 cut(s) 149, 749, 782, 871
HpySE526I ACGT 3 cut(s) 144, 340, 630
Hsp92II CATG 6 cut(s) 337, 365, 414, 482, 493, 767
HspAI GCGC 2 cut(s) 163, 300
Kzo9I GATC 2 cut(s) 57, 637
LweI GCATC 2 cut(s) 390, 523
MaeI CTAG 3 cut(s) 293, 723, 879
MaeII ACGT 3 cut(s) 144, 340, 630
MaeIII GTNAC 2 cut(s) 20, 690
MalI GATC 2 cut(s) 59, 639
MboI GATC 2 cut(s) 57, 637
MboII GAAGA 8 cut(s) 82, 85, 358, 524, 578, 647, 674, 781
MfeI CAATTG 1 cut(s) 793
MhlI GDGCHC 2 cut(s) 595, 792
MlsI TGGCCA 1 cut(s) 611
MluCI AATT 9 cut(s) 10, 303, 437, 498, 543, 604, 622, 645, 793
MluNI TGGCCA 1 cut(s) 611
MlyI GAGTC 2 cut(s) 407, 524
Mox20I TGGCCA 1 cut(s) 611
Mph1103I ATGCAT 1 cut(s) 383
MscI TGGCCA 1 cut(s) 611
MseI TTAA 3 cut(s) 347, 501, 552
MslI CAYNNNNRTG 1 cut(s) 483
Msp20I TGGCCA 1 cut(s) 611
MspI CCGG 3 cut(s) 32, 174, 467
MunI CAATTG 1 cut(s) 793
Mva1269I GAATGC 3 cut(s) 302, 383, 490
MwoI GCNNNNNNNGC 2 cut(s) 306, 570
NdeII GATC 2 cut(s) 57, 637
NlaIII CATG 6 cut(s) 337, 365, 414, 482, 493, 767
NmeAIII GCCGAG 1 cut(s) 599
NmuCI GTSAC 1 cut(s) 690
NsbI TGCGCA 1 cut(s) 301
NsiI ATGCAT 1 cut(s) 383
NspI RCATGY 3 cut(s) 337, 414, 767
PaeI GCATGC 1 cut(s) 337
PaeR7I CTCGAG 1 cut(s) 395
PagI TCATGA 1 cut(s) 478
PceI AGGCCT 2 cut(s) 213, 721
PciI ACATGT 1 cut(s) 410
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PctI GAATGC 3 cut(s) 302, 383, 490
PfeI GAWTC 1 cut(s) 875
PleI GAGTC 2 cut(s) 406, 523
PpsI GAGTC 2 cut(s) 406, 523
PscI ACATGT 1 cut(s) 410
PshBI ATTAAT 1 cut(s) 501
PspXI VCTCGAGB 1 cut(s) 395
RsaI GTAC 3 cut(s) 192, 287, 811
RsaNI GTAC 3 cut(s) 191, 286, 810
RseI CAYNNNNRTG 1 cut(s) 483
SaqAI TTAA 3 cut(s) 347, 501, 552
Sau3AI GATC 2 cut(s) 57, 637
SchI GAGTC 2 cut(s) 407, 524
SduI GDGCHC 2 cut(s) 595, 792
SfaNI GCATC 2 cut(s) 390, 523
Sfr274I CTCGAG 1 cut(s) 395
SlaI CTCGAG 1 cut(s) 395
SmiMI CAYNNNNRTG 1 cut(s) 483
SmlI CTYRAG 1 cut(s) 395
SmoI CTYRAG 1 cut(s) 395
SphI GCATGC 1 cut(s) 337
Sse9I AATT 9 cut(s) 10, 303, 437, 498, 543, 604, 622, 645, 793
SseBI AGGCCT 2 cut(s) 213, 721
SspMI CTAG 3 cut(s) 293, 723, 879
StuI AGGCCT 2 cut(s) 213, 721
TaaI ACNGT 5 cut(s) 64, 76, 290, 527, 909
TaiI ACGT 3 cut(s) 147, 343, 633
TaqI TCGA 6 cut(s) 56, 216, 384, 396, 686, 698
TasI AATT 9 cut(s) 10, 303, 437, 498, 543, 604, 622, 645, 793
TatI WGTACW 1 cut(s) 809
TfiI GAWTC 1 cut(s) 875
Tru1I TTAA 3 cut(s) 347, 501, 552
Tru9I TTAA 3 cut(s) 347, 501, 552
TscAI CASTG 1 cut(s) 67
TseFI GTSAC 1 cut(s) 690
Tsp45I GTSAC 1 cut(s) 690
TspDTI ATGAA 4 cut(s) 238, 519, 675, 684
TspGWI ACGGA 1 cut(s) 689
TspRI CASTG 1 cut(s) 67
VspI ATTAAT 1 cut(s) 501
XapI RAATTY 3 cut(s) 10, 604, 622
XbaI TCTAGA 1 cut(s) 292
XceI RCATGY 3 cut(s) 337, 414, 767
XhoI CTCGAG 1 cut(s) 395
XmiI GTMKAC 1 cut(s) 743
XspI CTAG 3 cut(s) 293, 723, 879
Zsp2I ATGCAT 1 cut(s) 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.