Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0016563

Physical Location & Seq

Genomic Coordinates Reverse (-)

4661099 .. 4662310

Gene Structure 1212 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1036300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1212 bp

ATGACTGACTGGAGAAAGCAATCCAACACACACAAAGTCCGATCCCAACACCAACACAAACAACATCACACTCTTCTCCTCGTAGCTTCTTCTCGTCAATATTACTATTACTATTATTGTTGTTTTCCGTCGTCTCGGAATATCCATGCACTAGGGCTGGCCGGAAGTGCAAGCCGATCTCCGGCCACAAGGTTAACATCAAGGTATTGCCGCTCAGTCTTCTTTATGCAGGGAATGTCATCATCGACCACGAATACAAATTACTCCCCAGTACCTCAACATGCCGCCTCCCCTCCAATTATCATCCTTCTGACCGTCCTCCTTGTCTTCTTCTTTGCGGGTTTCTTTTCTATTTACTTTTGCAAGTGCTTCATGGATCACATCCTTACCTCCCTTCAGCACACGCCCTCCGGAGACCTTGTGGGCACGGCGGTTGTCCCCAAAGAAGGCCTTGACGTTTCACTCATACAATCATTTCCAACGTTCGAGTACGCAAGTGTCAATGATTTCCGGATAGAGAAGTACGGCCTAGAATGCGCAATTTGCTTGGTGGAGTTTGAGGACGATAGCATACTACGCCTCTTGACCGAGTGTTGCCATGTTTTCCACAAAGATTGCATCGACCTTTGGCTCGAGTCTCATAAAACTTGTCCATTTTGCCGTGGAAATCTCGAAGTAGTAGCCCCGAGTTTAGCGGACAAGTCTCCGGTGCTTGCTCATGAGAACGACGCCATGCAAGACATTCATGTTGGGCATGAATCAGTGTTGTTGGATCTGGATGCTGTCCGCATCGACATTAAAGATGAACAAAGCCAAGATCATTGCCATCAAGGAAGTAATGACAGTAGTAATAACATTATGGTTTCACAAGGTGAACAAAATAGCAGTAGCACTACTCAAGATCGAGATCGAGATCAAGATCACAATCAGAATCATGATCGAAATGAAGAGAATGAAACCGAAAGATTTTCACGGTCGCATTCAACGGGGCACTCCATAGTTAGGCCTAGAGCAGAAGAGGATAGATATACGCTGAGATTGCCAGAGCATGTGAAGATAAAACTTATAAGAGGACATCATAACTGGACAGGAAGCTGTACTACATTTGGGGAGTTCTCTCGCCAAGGAGACAATGCAGGTGCTTCCCGCTTTGGAGAGGCCTCCGGCTCCGCCGGTGGAGGAGATATTAACAAATTAGCTTCTGTGTTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

404

Amino Acids

45.41

Weight (kDa)

6.1

Isoelectric Point (pI)

63.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	178 - 221	1.4e-13	Ring finger domain
zf-C3HC4_2	PF13923	178 - 220	6.1e-08	Zinc finger, C3HC4 type (RING finger)
zf-RING_11	PF17123	178 - 207	1.3e-07	RING-like zinc finger
zf-rbx1	PF12678	179 - 221	4.5e-07	RING-H2 zinc finger domain
zf-C3HC4	PF00097	179 - 220	2.6e-06	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016563)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G17920 AT5G46650
fragaria_vesca	FvH4_5g22380
malus_domestica	MD06G1036300.v1.1
prunus_persica	Prupe.5G033100_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0210121
rosa_laevigata	RLG00000003063
rosa_multiflora	Rmu_sc0025548.1_g000001
rosa_rugosa	Rorug07G0118700
rosa_samantha	Rh7AG252200 Rh7BG247000 Rh7CG269200 Rh7DG259500
rosa_wichuraiana	Rw7G021430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1067
AarI	CACCTGC	1 cut(s)	1127
Acc16I	TGCGCA	1 cut(s)	538
Acc36I	ACCTGC	1 cut(s)	1127
AccBSI	CCGCTC	1 cut(s)	213
AccIII	TCCGGA	2 cut(s)	410, 510
AciI	CCGC	8 cut(s)	211, 285, 338, 431, 695, 787, 1147, 1170
AclI	AACGTT	1 cut(s)	482
AclWI	GGATC	3 cut(s)	36, 384, 780
AcoI	YGGCCR	2 cut(s)	159, 183
AcuI	CTGAAG	1 cut(s)	380
AcyI	GRCGYC	1 cut(s)	729
AdeI	CACNNNGTG	1 cut(s)	872
AfaI	GTAC	4 cut(s)	273, 491, 524, 1099
AfiI	CCNNNNNNNGG	3 cut(s)	181, 446, 1002
AgsI	TTSAA	1 cut(s)	984
AluBI	AGCT	3 cut(s)	86, 1095, 1199
AluI	AGCT	3 cut(s)	86, 1095, 1199
Alw26I	GTCTC	5 cut(s)	138, 408, 642, 708, 1122
AlwI	GGATC	3 cut(s)	36, 384, 780
Ama87I	CYCGRG	2 cut(s)	632, 685
Aor13HI	TCCGGA	2 cut(s)	410, 510
AoxI	GGCC	6 cut(s)	159, 183, 448, 526, 1004, 1158
AspLEI	GCGC	1 cut(s)	539
AsuHPI	GGTGA	1 cut(s)	884
AvaI	CYCGRG	2 cut(s)	632, 685
BaeGI	GKGCMC	2 cut(s)	428, 993
BarI	GAAGNNNNNNTAC	2 cut(s)	1084, 1116
BbsI	GAAGAC	2 cut(s)	211, 319
BccI	CCATC	1 cut(s)	834
BceAI	ACGGC	3 cut(s)	444, 541, 645
BcoDI	GTCTC	5 cut(s)	138, 408, 642, 708, 1122
BfaI	CTAG	3 cut(s)	152, 530, 1008
BfuAI	ACCTGC	1 cut(s)	1127
BisI	GCNGC	2 cut(s)	211, 285
BlsI	GCNGC	2 cut(s)	212, 286
BmeT110I	CYCGRG	2 cut(s)	632, 685
BmiI	GGNNCC	1 cut(s)	1168
BmrI	ACTGGG	1 cut(s)	263
BmsI	GCATC	3 cut(s)	627, 769, 798
BmuI	ACTGGG	1 cut(s)	263
BpiI	GAAGAC	2 cut(s)	211, 319
BpmI	CTGGAG	1 cut(s)	31
BpuEI	CTTGAG	1 cut(s)	882
BsaBI	GATNNNNATC	4 cut(s)	906, 912, 918, 924
BsaHI	GRCGYC	1 cut(s)	729
BsaI	GGTCTC	1 cut(s)	408
BsaJI	CCNNGG	2 cut(s)	661, 1123
BsaWI	WCCGGW	3 cut(s)	410, 510, 706
BsaXI	ACNNNNNCTCC	4 cut(s)	392, 405, 422, 435
Bsc4I	CCNNNNNNNGG	3 cut(s)	181, 446, 1002
Bse118I	RCCGGY	1 cut(s)	1172
Bse1I	ACTGG	3 cut(s)	14, 269, 1088
Bse3DI	GCAATG	1 cut(s)	820
Bse8I	GATNNNNATC	4 cut(s)	906, 912, 918, 924
BseAI	TCCGGA	2 cut(s)	410, 510
BseDI	CCNNGG	2 cut(s)	661, 1123
BseGI	GGATG	3 cut(s)	303, 381, 784
BseJI	GATNNNNATC	4 cut(s)	906, 912, 918, 924
BseLI	CCNNNNNNNGG	3 cut(s)	181, 446, 1002
BseMI	GCAATG	1 cut(s)	820
BseMII	CTCAG	2 cut(s)	228, 1025
BseNI	ACTGG	3 cut(s)	14, 269, 1088
BseRI	GAGGAG	2 cut(s)	68, 1194
BseSI	GKGCMC	2 cut(s)	428, 993
Bsh1285I	CGRYCG	1 cut(s)	977
BshFI	GGCC	6 cut(s)	161, 185, 450, 528, 1006, 1160
BsiEI	CGRYCG	1 cut(s)	977
BsiHKCI	CYCGRG	2 cut(s)	632, 685
BsiSI	CCGG	7 cut(s)	162, 182, 411, 511, 707, 1164, 1173
BslFI	GGGAC	1 cut(s)	422
BslI	CCNNNNNNNGG	3 cut(s)	181, 446, 1002
BsmAI	GTCTC	5 cut(s)	138, 408, 642, 708, 1122
BsmBI	CGTCTC	1 cut(s)	138
BsmFI	GGGAC	1 cut(s)	422
BsmI	GAATGC	2 cut(s)	539, 979
BsnI	GGCC	6 cut(s)	161, 185, 450, 528, 1006, 1160
Bso31I	GGTCTC	1 cut(s)	408
BsoBI	CYCGRG	2 cut(s)	632, 685
Bsp1286I	GDGCHC	2 cut(s)	428, 993
Bsp13I	TCCGGA	2 cut(s)	410, 510
BspACI	CCGC	8 cut(s)	211, 285, 338, 431, 695, 787, 1147, 1170
BspANI	GGCC	6 cut(s)	161, 185, 450, 528, 1006, 1160
BspCNI	CTCAG	2 cut(s)	227, 1026
BspEI	TCCGGA	2 cut(s)	410, 510
BspHI	TCATGA	2 cut(s)	718, 934
BspLI	GGNNCC	1 cut(s)	1168
BspMI	ACCTGC	1 cut(s)	1127
BspPI	GGATC	3 cut(s)	36, 384, 780
BspTNI	GGTCTC	1 cut(s)	408
BsrBI	CCGCTC	1 cut(s)	213
BsrDI	GCAATG	1 cut(s)	820
BsrFI	RCCGGY	1 cut(s)	1172
BsrI	ACTGG	3 cut(s)	14, 269, 1088
BssAI	RCCGGY	1 cut(s)	1172
BssECI	CCNNGG	2 cut(s)	661, 1123
BssNI	GRCGYC	1 cut(s)	729
BssT1I	CCWWGG	1 cut(s)	1123
Bst4CI	ACNGT	3 cut(s)	316, 845, 975
Bst6I	CTCTTC	3 cut(s)	78, 942, 1011
BstACI	GRCGYC	1 cut(s)	729
BstC8I	GCNNGC	3 cut(s)	159, 172, 714
BstDEI	CTNAG	2 cut(s)	214, 1034
BstDSI	CCRYGG	1 cut(s)	661
BstF5I	GGATG	3 cut(s)	303, 381, 784
BstHHI	GCGC	1 cut(s)	539
BstMAI	GTCTC	5 cut(s)	138, 408, 642, 708, 1122
BstMCI	CGRYCG	1 cut(s)	977
BstMWI	GCNNNNNNNGC	5 cut(s)	167, 534, 543, 576, 1039
BstNSI	RCATGY	2 cut(s)	284, 1052
BstSLI	GKGCMC	2 cut(s)	428, 993
BstV2I	GAAGAC	2 cut(s)	211, 319
BstX2I	RGATCY	1 cut(s)	772
BstYI	RGATCY	1 cut(s)	772
BsuRI	GGCC	6 cut(s)	161, 185, 450, 528, 1006, 1160
BtgI	CCRYGG	1 cut(s)	661
BtsCI	GGATG	3 cut(s)	303, 381, 784
BtsIMutI	CAGTG	1 cut(s)	768
BveI	ACCTGC	1 cut(s)	1127
Cac8I	GCNNGC	3 cut(s)	159, 172, 714
CciI	TCATGA	2 cut(s)	718, 934
CfoI	GCGC	1 cut(s)	539
Cfr10I	RCCGGY	1 cut(s)	1172
CseI	GACGC	1 cut(s)	737
Csp6I	GTAC	4 cut(s)	272, 490, 523, 1098
CspCI	CAANNNNNGTGG	2 cut(s)	596, 631
CviQI	GTAC	4 cut(s)	272, 490, 523, 1098
DdeI	CTNAG	2 cut(s)	214, 1034
DraIII	CACNNNGTG	1 cut(s)	872
EaeI	YGGCCR	2 cut(s)	159, 183
Eam1104I	CTCTTC	3 cut(s)	78, 942, 1011
EarI	CTCTTC	3 cut(s)	78, 942, 1011
EciI	GGCGGA	1 cut(s)	1159
Eco130I	CCWWGG	1 cut(s)	1123
Eco147I	AGGCCT	3 cut(s)	450, 1006, 1160
Eco31I	GGTCTC	1 cut(s)	408
Eco57I	CTGAAG	1 cut(s)	380
Eco88I	CYCGRG	2 cut(s)	632, 685
EcoT14I	CCWWGG	1 cut(s)	1123
ErhI	CCWWGG	1 cut(s)	1123
Esp3I	CGTCTC	1 cut(s)	138
FalI	AAGNNNNNCTT	2 cut(s)	435, 467
FaqI	GGGAC	1 cut(s)	422
FauI	CCCGC	2 cut(s)	331, 1154
Fnu4HI	GCNGC	2 cut(s)	211, 285
FokI	GGATG	3 cut(s)	290, 368, 791
Fsp4HI	GCNGC	2 cut(s)	211, 285
FspBI	CTAG	3 cut(s)	152, 530, 1008
FspI	TGCGCA	1 cut(s)	538
GlaI	GCGC	1 cut(s)	538
GluI	GCNGC	2 cut(s)	211, 285
GsuI	CTGGAG	1 cut(s)	31
HaeIII	GGCC	6 cut(s)	161, 185, 450, 528, 1006, 1160
HapII	CCGG	7 cut(s)	162, 182, 411, 511, 707, 1164, 1173
HgaI	GACGC	1 cut(s)	737
HhaI	GCGC	1 cut(s)	539
Hin1I	GRCGYC	1 cut(s)	729
Hin6I	GCGC	1 cut(s)	537
HinP1I	GCGC	1 cut(s)	537
HincII	GTYRAC	1 cut(s)	195
HindII	GTYRAC	1 cut(s)	195
HinfI	GANTC	3 cut(s)	635, 758, 931
HpaI	GTTAAC	1 cut(s)	195
HpaII	CCGG	7 cut(s)	162, 182, 411, 511, 707, 1164, 1173
HphI	GGTGA	1 cut(s)	884
Hpy166II	GTNNAC	2 cut(s)	195, 875
Hpy188I	TCNGA	4 cut(s)	41, 138, 312, 930
Hpy8I	GTNNAC	2 cut(s)	195, 875
Hpy99I	CGWCG	2 cut(s)	133, 731
HpyAV	CCTTC	3 cut(s)	317, 404, 440
HpyCH4III	ACNGT	3 cut(s)	316, 845, 975
HpyCH4IV	ACGT	2 cut(s)	456, 482
HpyCH4V	TGCA	7 cut(s)	149, 170, 229, 363, 618, 736, 1136
HpyF10VI	GCNNNNNNNGC	5 cut(s)	167, 534, 543, 576, 1039
HpyF3I	CTNAG	2 cut(s)	214, 1034
HpySE526I	ACGT	2 cut(s)	456, 482
Hsp92I	GRCGYC	1 cut(s)	729
HspAI	GCGC	1 cut(s)	537
Kpn2I	TCCGGA	2 cut(s)	410, 510
KspAI	GTTAAC	1 cut(s)	195
LmnI	GCTCC	1 cut(s)	1172
LweI	GCATC	3 cut(s)	627, 769, 798
MaeI	CTAG	3 cut(s)	152, 530, 1008
MaeII	ACGT	2 cut(s)	456, 482
MbiI	CCGCTC	1 cut(s)	213
MboII	GAAGA	8 cut(s)	65, 81, 211, 319, 322, 959, 1028, 1066
MflI	RGATCY	1 cut(s)	772
MhlI	GDGCHC	2 cut(s)	428, 993
MluCI	AATT	4 cut(s)	259, 297, 540, 1193
MlyI	GAGTC	1 cut(s)	644
MmeI	TCCRAC	3 cut(s)	48, 503, 750
MroI	TCCGGA	2 cut(s)	410, 510
MseI	TTAA	4 cut(s)	194, 798, 1188, 1210
MspI	CCGG	7 cut(s)	162, 182, 411, 511, 707, 1164, 1173
Mva1269I	GAATGC	2 cut(s)	539, 979
MwoI	GCNNNNNNNGC	5 cut(s)	167, 534, 543, 576, 1039
NlaIV	GGNNCC	1 cut(s)	1168
NsbI	TGCGCA	1 cut(s)	538
NspI	RCATGY	2 cut(s)	284, 1052
PaeR7I	CTCGAG	1 cut(s)	632
PagI	TCATGA	2 cut(s)	718, 934
PaqCI	CACCTGC	1 cut(s)	1127
PceI	AGGCCT	3 cut(s)	450, 1006, 1160
PctI	GAATGC	2 cut(s)	539, 979
PfeI	GAWTC	2 cut(s)	758, 931
PkrI	GCNGC	2 cut(s)	212, 286
PleI	GAGTC	1 cut(s)	643
PpsI	GAGTC	1 cut(s)	643
PsiI	TTATAA	1 cut(s)	1067
Psp1406I	AACGTT	1 cut(s)	482
PspN4I	GGNNCC	1 cut(s)	1168
PspXI	VCTCGAGB	1 cut(s)	632
PsuI	RGATCY	1 cut(s)	772
RsaI	GTAC	4 cut(s)	273, 491, 524, 1099
RsaNI	GTAC	4 cut(s)	272, 490, 523, 1098
SaqAI	TTAA	4 cut(s)	194, 798, 1188, 1210
SatI	GCNGC	2 cut(s)	211, 285
SchI	GAGTC	1 cut(s)	644
SduI	GDGCHC	2 cut(s)	428, 993
SfaNI	GCATC	3 cut(s)	627, 769, 798
Sfr274I	CTCGAG	1 cut(s)	632
SgrAI	CRCCGGYG	1 cut(s)	1172
SlaI	CTCGAG	1 cut(s)	632
SmlI	CTYRAG	2 cut(s)	632, 897
SmoI	CTYRAG	2 cut(s)	632, 897
Sse9I	AATT	4 cut(s)	259, 297, 540, 1193
SseBI	AGGCCT	3 cut(s)	450, 1006, 1160
SsiI	CCGC	8 cut(s)	211, 285, 338, 431, 695, 787, 1147, 1170
SspI	AATATT	1 cut(s)	101
SspMI	CTAG	3 cut(s)	152, 530, 1008
StuI	AGGCCT	3 cut(s)	450, 1006, 1160
StyI	CCWWGG	1 cut(s)	1123
TaaI	ACNGT	3 cut(s)	316, 845, 975
TaiI	ACGT	2 cut(s)	459, 485
TaqI	TCGA	9 cut(s)	245, 486, 621, 633, 672, 792, 904, 910, 940
TaqII	GACCGA	1 cut(s)	602
TasI	AATT	4 cut(s)	259, 297, 540, 1193
TatI	WGTACW	1 cut(s)	1097
TauI	GCSGC	2 cut(s)	213, 287
TfiI	GAWTC	2 cut(s)	758, 931
Tru1I	TTAA	4 cut(s)	194, 798, 1188, 1210
Tru9I	TTAA	4 cut(s)	194, 798, 1188, 1210
TscAI	CASTG	1 cut(s)	768
TspDTI	ATGAA	6 cut(s)	361, 734, 771, 819, 960, 969
TspGWI	ACGGA	1 cut(s)	117
TspRI	CASTG	1 cut(s)	768
XceI	RCATGY	2 cut(s)	284, 1052
XhoI	CTCGAG	1 cut(s)	632
XspI	CTAG	3 cut(s)	152, 530, 1008

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.