Rw7G021430

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0016563
Physical Location & Seq
Reverse (-)
24892097 .. 24893020
924 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G021430.1

Sequence Viewer

Length: 924 bp
ATGCTGGGAATGTCAACGAATTCTAATAGTTACCCTCCACCGGAAAATATCAGTTCCTCTCCATCGATCACTGTCCTTTTGACCGTCCTCCTCCTCGTCTTCTTCTTTATTGGTTTCTTTTCTATTTACTTTTGCAAGTGTTTCATGGAAAACGTCCTCACGTCTTTTCAGCGCACACCCTCCGGCGACCTTGTGGGCGTACCTGTTCCTCCCAAAGAAGGCCTAGACCTCTCTCTAATACAATCATTTCCCACCTTCATCTACGCAAGTGTCAAAGATTTTCGCATAGAGAAGTACGGTTTAGAATGCGCAATTTGCTTGGTGGAGTTTGAGGATGATAGCATTCTACGTCTTTTAACAGCTTGTTGCCATGTCTTCCACCAAGAATGCATCGACCTGTGGCTCGAGTCTCACAAAACATGTCCCTTTTGCCGTGGAAACCTTGAATTGTCGCCAACTATAATGGACAAGTCTCCGGTGCTTGCTCATGAGAATGCCATGCACGAAATTAATGAAGGTGAAGAGTCCATATACGGTCAAGATGCTATCCGAATTGACATTAAAGACGAAGACGAAGAAGGCCGAGAAGGATATCGTAGTGCTCATCAACAAAATTTGGCTGCTAATGTAAATTCTAACGTGGGAGGCCAACAAATTGATGGACAAAATGAAGAGAACGAAACGGAAAGATTTTCGAGGTCACACTCGACAGGACACTCCATAGTTAGGCCTAGACAAGAGGAGGATAGGTATACACTCAGATTACCAGAGCATGTGAAGATAAAACTTCTTAGAGGGCACAATTGGACAGGAAGTTGTACTACATTTGGGGAGTTTTCACGACAAAGAGGCAGTTGTAAAGGTGGCTTTGGAGAGGTCTCAGAATCTAGCTCTGCTGATGGAGAGATTAGTAGCAAACTGTAA

Protein Analysis

307

Amino Acids

34.42

Weight (kDa)

5.0

Isoelectric Point (pI)

61.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 102 - 145 1e-13 Ring finger domain
zf-C3HC4_2 PF13923 102 - 144 3.7e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 102 - 131 1.4e-07 RING-like zinc finger
zf-C3HC4 PF00097 103 - 144 8.9e-06 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 106 - 145 1e-06 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016563)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17920 AT5G46650
fragaria_vesca FvH4_5g22380
malus_domestica MD06G1036300.v1.1
prunus_persica Prupe.5G033100_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0210121
rosa_laevigata RLG00000003063
rosa_multiflora Rmu_sc0025548.1_g000001
rosa_rugosa Rorug07G0118700
rosa_samantha Rh7AG252200 Rh7BG247000 Rh7CG269200 Rh7DG259500
rosa_wichuraiana Rw7G021430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 310
AccI GTMKAC 1 cut(s) 752
AcsI RAATTY 3 cut(s) 19, 613, 631
AfaI GTAC 3 cut(s) 201, 296, 820
AfiI CCNNNNNNNGG 3 cut(s) 40, 218, 726
AflIII ACRYGT 1 cut(s) 419
AgsI TTSAA 1 cut(s) 446
AjiI CACGTC 1 cut(s) 162
AluBI AGCT 2 cut(s) 362, 891
AluI AGCT 2 cut(s) 362, 891
Alw21I GWGCWC 1 cut(s) 604
Alw26I GTCTC 3 cut(s) 414, 477, 883
Ama87I CYCGRG 1 cut(s) 404
AoxI GGCC 4 cut(s) 220, 580, 646, 728
ApeKI GCWGC 1 cut(s) 620
ApoI RAATTY 3 cut(s) 19, 613, 631
AseI ATTAAT 1 cut(s) 510
AspLEI GCGC 2 cut(s) 174, 311
AsuHPI GGTGA 1 cut(s) 530
AvaI CYCGRG 1 cut(s) 404
BaeGI GKGCMC 1 cut(s) 801
BarI GAAGNNNNNNTAC 2 cut(s) 805, 837
BbsI GAAGAC 3 cut(s) 91, 367, 576
Bbv12I GWGCWC 1 cut(s) 604
BbvI GCAGC 1 cut(s) 607
BccI CCATC 3 cut(s) 70, 653, 893
BceAI ACGGC 1 cut(s) 417
BcoDI GTCTC 3 cut(s) 414, 477, 883
BfaI CTAG 3 cut(s) 224, 732, 888
BisI GCNGC 1 cut(s) 621
BlsI GCNGC 1 cut(s) 622
BmeT110I CYCGRG 1 cut(s) 404
BmgBI CACGTC 1 cut(s) 162
BmsI GCATC 2 cut(s) 399, 532
BpiI GAAGAC 3 cut(s) 91, 367, 576
Bsa29I ATCGAT 1 cut(s) 65
BsaI GGTCTC 1 cut(s) 883
BsaJI CCNNGG 1 cut(s) 433
BsaWI WCCGGW 2 cut(s) 40, 475
BsaXI ACNNNNNCTCC 2 cut(s) 734, 764
Bsc4I CCNNNNNNNGG 3 cut(s) 40, 218, 726
BseCI ATCGAT 1 cut(s) 65
BseDI CCNNGG 1 cut(s) 433
BseGI GGATG 1 cut(s) 340
BseLI CCNNNNNNNGG 3 cut(s) 40, 218, 726
BseMII CTCAG 2 cut(s) 772, 894
BseRI GAGGAG 3 cut(s) 80, 83, 755
BseSI GKGCMC 1 cut(s) 801
BseXI GCAGC 1 cut(s) 607
BseYI CCCAGC 1 cut(s) 4
BshFI GGCC 4 cut(s) 222, 582, 648, 730
BshVI ATCGAT 1 cut(s) 65
BsiHKAI GWGCWC 1 cut(s) 604
BsiHKCI CYCGRG 1 cut(s) 404
BsiSI CCGG 3 cut(s) 41, 183, 476
BslFI GGGAC 1 cut(s) 408
BslI CCNNNNNNNGG 3 cut(s) 40, 218, 726
BsmAI GTCTC 3 cut(s) 414, 477, 883
BsmFI GGGAC 1 cut(s) 408
BsmI GAATGC 4 cut(s) 311, 342, 392, 499
BsnI GGCC 4 cut(s) 222, 582, 648, 730
Bso31I GGTCTC 1 cut(s) 883
BsoBI CYCGRG 1 cut(s) 404
Bsp1286I GDGCHC 2 cut(s) 604, 801
Bsp143I GATC 1 cut(s) 66
BspANI GGCC 4 cut(s) 222, 582, 648, 730
BspCNI CTCAG 2 cut(s) 771, 893
BspDI ATCGAT 1 cut(s) 65
BspHI TCATGA 1 cut(s) 487
BspTNI GGTCTC 1 cut(s) 883
BssECI CCNNGG 1 cut(s) 433
BssMI GATC 1 cut(s) 66
BssNAI GTATAC 1 cut(s) 753
Bst1107I GTATAC 1 cut(s) 753
Bst4CI ACNGT 5 cut(s) 73, 85, 299, 536, 921
Bst6I CTCTTC 2 cut(s) 516, 666
BstC8I GCNNGC 1 cut(s) 483
BstDEI CTNAG 3 cut(s) 758, 791, 880
BstDSI CCRYGG 1 cut(s) 433
BstF5I GGATG 1 cut(s) 340
BstHHI GCGC 2 cut(s) 174, 311
BstKTI GATC 1 cut(s) 69
BstMAI GTCTC 3 cut(s) 414, 477, 883
BstMBI GATC 1 cut(s) 66
BstMWI GCNNNNNNNGC 1 cut(s) 315
BstNSI RCATGY 2 cut(s) 423, 776
BstSLI GKGCMC 1 cut(s) 801
BstV1I GCAGC 1 cut(s) 607
BstV2I GAAGAC 3 cut(s) 91, 367, 576
BstZ17I GTATAC 1 cut(s) 753
Bsu15I ATCGAT 1 cut(s) 65
BsuRI GGCC 4 cut(s) 222, 582, 648, 730
BsuTUI ATCGAT 1 cut(s) 65
BtgI CCRYGG 1 cut(s) 433
BtrI CACGTC 1 cut(s) 162
BtsCI GGATG 1 cut(s) 340
BtsIMutI CAGTG 1 cut(s) 69
Cac8I GCNNGC 1 cut(s) 483
CciI TCATGA 1 cut(s) 487
CfoI GCGC 2 cut(s) 174, 311
ClaI ATCGAT 1 cut(s) 65
Csp6I GTAC 3 cut(s) 200, 295, 819
CviAII CATG 6 cut(s) 145, 371, 420, 488, 499, 773
CviJI RGCY 9 cut(s) 222, 362, 403, 582, 620, 648, 730, 867, 891
CviKI_1 RGCY 9 cut(s) 222, 362, 403, 582, 620, 648, 730, 867, 891
CviQI GTAC 3 cut(s) 200, 295, 819
DdeI CTNAG 3 cut(s) 758, 791, 880
DpnI GATC 1 cut(s) 68
DpnII GATC 1 cut(s) 66
Eam1104I CTCTTC 2 cut(s) 516, 666
EarI CTCTTC 2 cut(s) 516, 666
Eco147I AGGCCT 2 cut(s) 222, 730
Eco31I GGTCTC 1 cut(s) 883
Eco32I GATATC 1 cut(s) 593
Eco88I CYCGRG 1 cut(s) 404
EcoRI GAATTC 1 cut(s) 19
EcoRV GATATC 1 cut(s) 593
EcoT22I ATGCAT 1 cut(s) 392
FaeI CATG 6 cut(s) 148, 374, 423, 491, 502, 776
FaqI GGGAC 1 cut(s) 408
FatI CATG 6 cut(s) 144, 370, 419, 487, 498, 772
FblI GTMKAC 1 cut(s) 752
Fnu4HI GCNGC 1 cut(s) 621
FokI GGATG 1 cut(s) 347
Fsp4HI GCNGC 1 cut(s) 621
FspBI CTAG 3 cut(s) 224, 732, 888
FspI TGCGCA 1 cut(s) 310
GlaI GCGC 2 cut(s) 173, 310
GluI GCNGC 1 cut(s) 621
GsaI CCCAGC 1 cut(s) 8
HaeIII GGCC 4 cut(s) 222, 582, 648, 730
HapII CCGG 3 cut(s) 41, 183, 476
HhaI GCGC 2 cut(s) 174, 311
Hin1II CATG 6 cut(s) 148, 374, 423, 491, 502, 776
Hin6I GCGC 2 cut(s) 172, 309
HinP1I GCGC 2 cut(s) 172, 309
HincII GTYRAC 1 cut(s) 15
HindII GTYRAC 1 cut(s) 15
HinfI GANTC 3 cut(s) 407, 524, 884
HpaII CCGG 3 cut(s) 41, 183, 476
HphI GGTGA 1 cut(s) 530
Hpy166II GTNNAC 2 cut(s) 15, 753
Hpy188I TCNGA 3 cut(s) 551, 761, 883
Hpy188III TCNNGA 3 cut(s) 488, 539, 840
Hpy8I GTNNAC 2 cut(s) 15, 753
HpyAV CCTTC 5 cut(s) 212, 265, 509, 572, 581
HpyCH4III ACNGT 5 cut(s) 73, 85, 299, 536, 921
HpyCH4IV ACGT 4 cut(s) 153, 161, 349, 639
HpyCH4V TGCA 3 cut(s) 135, 390, 502
HpyF10VI GCNNNNNNNGC 1 cut(s) 315
HpyF3I CTNAG 3 cut(s) 758, 791, 880
HpySE526I ACGT 4 cut(s) 153, 161, 349, 639
Hsp92II CATG 6 cut(s) 148, 374, 423, 491, 502, 776
HspAI GCGC 2 cut(s) 172, 309
Kzo9I GATC 1 cut(s) 66
LpnPI CCDG 8 cut(s) 54, 196, 216, 410, 489, 696, 780, 795
Lsp1109I GCAGC 1 cut(s) 607
LweI GCATC 2 cut(s) 399, 532
MaeI CTAG 3 cut(s) 224, 732, 888
MaeII ACGT 4 cut(s) 153, 161, 349, 639
MaeIII GTNAC 2 cut(s) 29, 699
MalI GATC 1 cut(s) 68
MboI GATC 1 cut(s) 66
MboII GAAGA 8 cut(s) 91, 94, 367, 533, 581, 587, 683, 790
MfeI CAATTG 1 cut(s) 802
MhlI GDGCHC 2 cut(s) 604, 801
MluCI AATT 9 cut(s) 19, 312, 446, 507, 552, 613, 631, 654, 802
MlyI GAGTC 2 cut(s) 416, 533
Mph1103I ATGCAT 1 cut(s) 392
MseI TTAA 3 cut(s) 356, 510, 561
MslI CAYNNNNRTG 1 cut(s) 492
MspI CCGG 3 cut(s) 41, 183, 476
MunI CAATTG 1 cut(s) 802
Mva1269I GAATGC 4 cut(s) 311, 342, 392, 499
MwoI GCNNNNNNNGC 1 cut(s) 315
NdeII GATC 1 cut(s) 66
NlaIII CATG 6 cut(s) 148, 374, 423, 491, 502, 776
NmeAIII GCCGAG 1 cut(s) 608
NmuCI GTSAC 1 cut(s) 699
NsbI TGCGCA 1 cut(s) 310
NsiI ATGCAT 1 cut(s) 392
NspI RCATGY 2 cut(s) 423, 776
PaeR7I CTCGAG 1 cut(s) 404
PagI TCATGA 1 cut(s) 487
PceI AGGCCT 2 cut(s) 222, 730
PciI ACATGT 1 cut(s) 419
PctI GAATGC 4 cut(s) 311, 342, 392, 499
PfeI GAWTC 1 cut(s) 884
PkrI GCNGC 1 cut(s) 622
PleI GAGTC 2 cut(s) 415, 532
PpsI GAGTC 2 cut(s) 415, 532
PscI ACATGT 1 cut(s) 419
PshBI ATTAAT 1 cut(s) 510
PspFI CCCAGC 1 cut(s) 4
PspXI VCTCGAGB 1 cut(s) 404
RsaI GTAC 3 cut(s) 201, 296, 820
RsaNI GTAC 3 cut(s) 200, 295, 819
RseI CAYNNNNRTG 1 cut(s) 492
SaqAI TTAA 3 cut(s) 356, 510, 561
SatI GCNGC 1 cut(s) 621
Sau3AI GATC 1 cut(s) 66
SchI GAGTC 2 cut(s) 416, 533
SduI GDGCHC 2 cut(s) 604, 801
SfaNI GCATC 2 cut(s) 399, 532
Sfr274I CTCGAG 1 cut(s) 404
SlaI CTCGAG 1 cut(s) 404
SmiMI CAYNNNNRTG 1 cut(s) 492
SmlI CTYRAG 1 cut(s) 404
SmoI CTYRAG 1 cut(s) 404
Sse9I AATT 9 cut(s) 19, 312, 446, 507, 552, 613, 631, 654, 802
SseBI AGGCCT 2 cut(s) 222, 730
SspMI CTAG 3 cut(s) 224, 732, 888
StuI AGGCCT 2 cut(s) 222, 730
TaaI ACNGT 5 cut(s) 73, 85, 299, 536, 921
TaiI ACGT 4 cut(s) 156, 164, 352, 642
TaqI TCGA 5 cut(s) 65, 393, 405, 695, 707
TasI AATT 9 cut(s) 19, 312, 446, 507, 552, 613, 631, 654, 802
TatI WGTACW 1 cut(s) 818
TfiI GAWTC 1 cut(s) 884
Tru1I TTAA 3 cut(s) 356, 510, 561
Tru9I TTAA 3 cut(s) 356, 510, 561
TscAI CASTG 1 cut(s) 76
TseFI GTSAC 1 cut(s) 699
TseI GCWGC 1 cut(s) 620
Tsp45I GTSAC 1 cut(s) 699
TspDTI ATGAA 4 cut(s) 133, 247, 528, 684
TspGWI ACGGA 1 cut(s) 698
TspRI CASTG 1 cut(s) 76
VspI ATTAAT 1 cut(s) 510
XapI RAATTY 3 cut(s) 19, 613, 631
XceI RCATGY 2 cut(s) 423, 776
XcmI CCANNNNNNNNNTGG 1 cut(s) 656
XhoI CTCGAG 1 cut(s) 404
XmiI GTMKAC 1 cut(s) 752
XspI CTAG 3 cut(s) 224, 732, 888
Zsp2I ATGCAT 1 cut(s) 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.