AT5G48810

Cytochrome b5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013843
Physical Location & Seq
Reverse (-)
19789067 .. 19790372
1306 bp
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UTR
Exon/CDS
Intron
AT5G48810.1

Sequence Viewer

Length: 423 bp
ATGGGCGGAGACGGAAAAGTTTTCACCTTGTCCGAGGTTTCTCAGCACAGTAGCGCCAAGGATTGTTGGATCGTCATCGACGGCAAGGTTTATGATGTGACAAAGTTCTTGGATGATCATCCTGGTGGTGATGAGGTTATCTTGACTTCTACAGGGAAAGATGCGACCGATGATTTCGAGGATGTGGGACATAGTTCGACTGCGAAAGCCATGCTAGATGAGTACTATGTGGGTGATATTGACACAGCTACTGTGCCGGTTAAAGCTAAGTTTGTGCCTCCTACGTCGACGAAAGCCGTGGCTACTCAGGATAAGAGCTCGGATTTTGTTATTAAGCTCCTTCAGTTCCTTGTTCCACTTCTAATCTTAGGCTTGGCTTTCGGCATTCGGTACTACACTAAGACCAAGGCTCCTTCTTCTTGA

Protein Analysis

140

Amino Acids

15.1

Weight (kDa)

4.87

Isoelectric Point (pI)

28.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 9 - 80 5.3e-31 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013843)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48810
fragaria_vesca FvH4_2g19040
malus_domestica MD05G1175200.v1.1 MD10G1164300.v1.1
prunus_persica Prupe.8G202900_v2.0.a1 Prupe.8G202900_v2.0.a1
pyrus_communis pycom05g16210 pycom10g14170
rosa_chinensis RchiOBHm_Chr6g0284851
rosa_laevigata RLG00000012707
rosa_multiflora Rmu_sc0000686.1_g000017
rosa_roxburghii Rroxscaffold_7G00184300
rosa_rugosa Rorug06G0167500
rosa_samantha Rh6AG280200 Rh6BG280500 Rh6CG282500
rosa_wichuraiana Rw6G024080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 287
AciI CCGC 1 cut(s) 6
AclWI GGATC 1 cut(s) 77
AcuI CTGAAG 1 cut(s) 326
AfaI GTAC 2 cut(s) 224, 392
AjnI CCWGG 1 cut(s) 121
AluBI AGCT 4 cut(s) 248, 266, 318, 337
AluI AGCT 4 cut(s) 248, 266, 318, 337
Alw21I GWGCWC 1 cut(s) 320
Alw26I GTCTC 1 cut(s) 3
AlwI GGATC 1 cut(s) 77
AlwNI CAGNNNCTG 1 cut(s) 251
AspLEI GCGC 1 cut(s) 56
AsuHPI GGTGA 3 cut(s) 16, 140, 245
BanII GRGCYC 1 cut(s) 320
Bbv12I GWGCWC 1 cut(s) 320
BceAI ACGGC 2 cut(s) 97, 281
BcgI CGANNNNNNTGC 2 cut(s) 193, 227
BciT130I CCWGG 1 cut(s) 123
BclI TGATCA 1 cut(s) 115
BcoDI GTCTC 1 cut(s) 3
BfaI CTAG 1 cut(s) 215
BfmI CTRYAG 1 cut(s) 150
BfoI RGCGCY 1 cut(s) 57
BmcAI AGTACT 1 cut(s) 224
Bme1390I CCNGG 1 cut(s) 123
BmiI GGNNCC 1 cut(s) 411
BmrFI CCNGG 1 cut(s) 123
BmsI GCATC 1 cut(s) 151
BsaBI GATNNNNATC 2 cut(s) 74, 117
BsaJI CCNNGG 4 cut(s) 33, 57, 297, 405
BsaXI ACNNNNNCTCC 1 cut(s) 394
Bse118I RCCGGY 1 cut(s) 256
Bse8I GATNNNNATC 2 cut(s) 74, 117
BseBI CCWGG 1 cut(s) 123
BseDI CCNNGG 4 cut(s) 33, 57, 297, 405
BseGI GGATG 3 cut(s) 118, 118, 187
BseJI GATNNNNATC 2 cut(s) 74, 117
BseMII CTCAG 2 cut(s) 56, 320
Bsh1285I CGRYCG 1 cut(s) 168
BsiEI CGRYCG 1 cut(s) 168
BsiHKAI GWGCWC 1 cut(s) 320
BsiSI CCGG 1 cut(s) 257
BslFI GGGAC 1 cut(s) 201
BsmAI GTCTC 1 cut(s) 3
BsmBI CGTCTC 1 cut(s) 3
BsmFI GGGAC 1 cut(s) 201
BsmI GAATGC 1 cut(s) 384
Bsp1286I GDGCHC 1 cut(s) 320
Bsp143I GATC 2 cut(s) 69, 115
BspACI CCGC 1 cut(s) 6
BspCNI CTCAG 2 cut(s) 55, 319
BspLI GGNNCC 1 cut(s) 411
BspPI GGATC 1 cut(s) 77
BsrFI RCCGGY 1 cut(s) 256
BssAI RCCGGY 1 cut(s) 256
BssECI CCNNGG 4 cut(s) 33, 57, 297, 405
BssMI GATC 2 cut(s) 69, 115
BssT1I CCWWGG 2 cut(s) 57, 405
Bst2UI CCWGG 1 cut(s) 123
Bst4CI ACNGT 2 cut(s) 50, 253
BstDEI CTNAG 5 cut(s) 42, 267, 306, 367, 399
BstDSI CCRYGG 1 cut(s) 297
BstF5I GGATG 3 cut(s) 118, 118, 187
BstH2I RGCGCY 1 cut(s) 57
BstHHI GCGC 1 cut(s) 56
BstKTI GATC 2 cut(s) 72, 118
BstMAI GTCTC 1 cut(s) 3
BstMBI GATC 2 cut(s) 69, 115
BstMCI CGRYCG 1 cut(s) 168
BstNI CCWGG 1 cut(s) 123
BstSCI CCNGG 1 cut(s) 121
BstSFI CTRYAG 1 cut(s) 150
BtgI CCRYGG 1 cut(s) 297
BtsCI GGATG 3 cut(s) 118, 118, 187
CaiI CAGNNNCTG 1 cut(s) 251
CfoI GCGC 1 cut(s) 56
Cfr10I RCCGGY 1 cut(s) 256
Csp6I GTAC 2 cut(s) 223, 391
CviAII CATG 1 cut(s) 211
CviQI GTAC 2 cut(s) 223, 391
DdeI CTNAG 5 cut(s) 42, 267, 306, 367, 399
DpnI GATC 2 cut(s) 71, 117
DpnII GATC 2 cut(s) 69, 115
EciI GGCGGA 1 cut(s) 21
Ecl136II GAGCTC 1 cut(s) 318
Eco130I CCWWGG 2 cut(s) 57, 405
Eco24I GRGCYC 1 cut(s) 320
Eco53kI GAGCTC 1 cut(s) 318
Eco57I CTGAAG 1 cut(s) 326
EcoICRI GAGCTC 1 cut(s) 318
EcoRII CCWGG 1 cut(s) 121
EcoT14I CCWWGG 2 cut(s) 57, 405
EcoT38I GRGCYC 1 cut(s) 320
ErhI CCWWGG 2 cut(s) 57, 405
Esp3I CGTCTC 1 cut(s) 3
FaeI CATG 1 cut(s) 214
FaiI YATR 4 cut(s) 93, 192, 212, 228
FaqI GGGAC 1 cut(s) 201
FatI CATG 1 cut(s) 210
FbaI TGATCA 1 cut(s) 115
FblI GTMKAC 1 cut(s) 287
FokI GGATG 3 cut(s) 105, 125, 194
FriOI GRGCYC 1 cut(s) 320
FspBI CTAG 1 cut(s) 215
GlaI GCGC 1 cut(s) 55
HaeII RGCGCY 1 cut(s) 57
HapII CCGG 1 cut(s) 257
HhaI GCGC 1 cut(s) 56
Hin1II CATG 1 cut(s) 214
Hin6I GCGC 1 cut(s) 54
HinP1I GCGC 1 cut(s) 54
HincII GTYRAC 1 cut(s) 288
HindII GTYRAC 1 cut(s) 288
HpaII CCGG 1 cut(s) 257
HphI GGTGA 3 cut(s) 16, 140, 245
Hpy166II GTNNAC 1 cut(s) 288
Hpy188I TCNGA 2 cut(s) 34, 322
Hpy188III TCNNGA 3 cut(s) 142, 308, 420
Hpy8I GTNNAC 1 cut(s) 288
Hpy99I CGWCG 3 cut(s) 83, 289, 292
HpyAV CCTTC 2 cut(s) 350, 423
HpyCH4III ACNGT 2 cut(s) 50, 253
HpyCH4IV ACGT 1 cut(s) 284
HpyF3I CTNAG 5 cut(s) 42, 267, 306, 367, 399
HpySE526I ACGT 1 cut(s) 284
Hsp92II CATG 1 cut(s) 214
HspAI GCGC 1 cut(s) 54
Ksp22I TGATCA 1 cut(s) 115
Kzo9I GATC 2 cut(s) 69, 115
LmnI GCTCC 2 cut(s) 342, 415
LpnPI CCDG 5 cut(s) 108, 135, 138, 270, 293
LweI GCATC 1 cut(s) 151
MaeI CTAG 1 cut(s) 215
MaeII ACGT 1 cut(s) 284
MaeIII GTNAC 1 cut(s) 97
MalI GATC 2 cut(s) 71, 117
MboI GATC 2 cut(s) 69, 115
MboII GAAGA 1 cut(s) 408
MhlI GDGCHC 1 cut(s) 320
MmeI TCCRAC 1 cut(s) 47
MnlI CCTC 4 cut(s) 28, 127, 172, 288
MseI TTAA 2 cut(s) 261, 333
MslI CAYNNNNRTG 1 cut(s) 123
MspI CCGG 1 cut(s) 257
MspR9I CCNGG 1 cut(s) 123
Mva1269I GAATGC 1 cut(s) 384
MvaI CCWGG 1 cut(s) 123
NdeII GATC 2 cut(s) 69, 115
NlaIII CATG 1 cut(s) 214
NlaIV GGNNCC 1 cut(s) 411
NmuCI GTSAC 1 cut(s) 97
PctI GAATGC 1 cut(s) 384
Psp124BI GAGCTC 1 cut(s) 320
Psp6I CCWGG 1 cut(s) 121
PspGI CCWGG 1 cut(s) 121
PspN4I GGNNCC 1 cut(s) 411
PstNI CAGNNNCTG 1 cut(s) 251
RsaI GTAC 2 cut(s) 224, 392
RsaNI GTAC 2 cut(s) 223, 391
RseI CAYNNNNRTG 1 cut(s) 123
SacI GAGCTC 1 cut(s) 320
SalI GTCGAC 1 cut(s) 286
SaqAI TTAA 2 cut(s) 261, 333
Sau3AI GATC 2 cut(s) 69, 115
ScaI AGTACT 1 cut(s) 224
ScrFI CCNGG 1 cut(s) 123
SduI GDGCHC 1 cut(s) 320
SetI ASST 9 cut(s) 29, 39, 90, 138, 250, 268, 287, 320, 339
SfaNI GCATC 1 cut(s) 151
SfcI CTRYAG 1 cut(s) 150
SgrDI CGTCGACG 1 cut(s) 286
SmiMI CAYNNNNRTG 1 cut(s) 123
SsiI CCGC 1 cut(s) 6
SspMI CTAG 1 cut(s) 215
SstI GAGCTC 1 cut(s) 320
StyD4I CCNGG 1 cut(s) 121
StyI CCWWGG 2 cut(s) 57, 405
TaaI ACNGT 2 cut(s) 50, 253
TaiI ACGT 1 cut(s) 287
TaqI TCGA 4 cut(s) 78, 177, 197, 287
TaqII GACCGA 1 cut(s) 182
TatI WGTACW 1 cut(s) 222
Tru1I TTAA 2 cut(s) 261, 333
Tru9I TTAA 2 cut(s) 261, 333
TseFI GTSAC 1 cut(s) 97
Tsp45I GTSAC 1 cut(s) 97
TspGWI ACGGA 1 cut(s) 27
XmiI GTMKAC 1 cut(s) 287
XspI CTAG 1 cut(s) 215
ZrmI AGTACT 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.