Rh6BG280500

Cytochrome b5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0013843
Physical Location & Seq
Forward (+)
50235176 .. 50237091
1916 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG280500.1

Sequence Viewer

Length: 606 bp
ATGGGAGTTGGTGTGGACAAAGCCCTCCCACCATTGCCCCATTCGCAATTTAATTTTGTTTGGCCTCACCAACCCAACCCAACCCAAACCCACCTTTCTCACCTTTATTATTTTCCCATTCAAATCCAACACGGACTCTGTCAGTGGAACTCTCTTGATCAGTTTCAAAAAACAGCTCTGTCTTCTGTAACAATGGGCGGGGAGAAAAAGGTCTACACTTTGGACGAGGTCTACAACCACAGCGACCGCAAAGACTGCTGGCTCGTCATTGATGGCAAGGTGTATGATGTGACAAAATTTTTGGAGGACCATCCTGGTGGTGATGAGGTCTTATTGTCATCCACAGGGAAGGATGCAACTGATGATTTTGAGGATGTTGGTCACAGTACTGCAGCTAGAGCAATGATGGATGAGTACTATGTAGGAGACATTGATAAAGCATTCATCTCAGCCAAGTCGACAACGTACACTCCTCCCAAGCAGCCTCAGTACAATCCGGACAAAACAACCGATTTCATCATCAGGCTCCTCCAGTTCCTTGTTCCCCTGCTCATTTTGGGTTTGGCTTTTGTTGTGCGCTCCTATACCAAATCACCAACAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyt-b5

Protein Analysis

201

Amino Acids

22.67

Weight (kDa)

5.52

Isoelectric Point (pI)

22.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 73 - 144 6.9e-29 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013843)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48810
fragaria_vesca FvH4_2g19040
malus_domestica MD05G1175200.v1.1 MD10G1164300.v1.1
prunus_persica Prupe.8G202900_v2.0.a1 Prupe.8G202900_v2.0.a1
pyrus_communis pycom05g16210 pycom10g14170
rosa_chinensis RchiOBHm_Chr6g0284851
rosa_laevigata RLG00000012707
rosa_multiflora Rmu_sc0000686.1_g000017
rosa_roxburghii Rroxscaffold_7G00184300
rosa_rugosa Rorug06G0167500
rosa_samantha Rh6AG280200 Rh6BG280500 Rh6CG282500
rosa_wichuraiana Rw6G024080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 213, 231, 458
AccIII TCCGGA 1 cut(s) 496
AciI CCGC 2 cut(s) 198, 247
AcsI RAATTY 1 cut(s) 296
AfaI GTAC 4 cut(s) 388, 416, 467, 491
AgsI TTSAA 2 cut(s) 122, 167
AjnI CCWGG 1 cut(s) 313
AluBI AGCT 2 cut(s) 176, 395
AluI AGCT 2 cut(s) 176, 395
Alw26I GTCTC 1 cut(s) 420
Aor13HI TCCGGA 1 cut(s) 496
AoxI GGCC 1 cut(s) 62
ApeKI GCWGC 2 cut(s) 392, 481
ApoI RAATTY 1 cut(s) 296
ArsI GACNNNNNNTTYG 2 cut(s) 283, 315
AspLEI GCGC 1 cut(s) 579
AspS9I GGNCC 1 cut(s) 307
AsuHPI GGTGA 4 cut(s) 59, 92, 332, 585
AvaII GGWCC 1 cut(s) 307
BbsI GAAGAC 1 cut(s) 174
BbvI GCAGC 2 cut(s) 404, 493
BccI CCATC 3 cut(s) 266, 318, 400
BciT130I CCWGG 1 cut(s) 315
BclI TGATCA 1 cut(s) 157
BcoDI GTCTC 1 cut(s) 420
BfaI CTAG 1 cut(s) 396
BfmI CTRYAG 1 cut(s) 390
BisI GCNGC 2 cut(s) 393, 482
BlsI GCNGC 2 cut(s) 394, 483
BmcAI AGTACT 2 cut(s) 388, 416
Bme1390I CCNGG 1 cut(s) 315
Bme18I GGWCC 1 cut(s) 307
BmgT120I GGNCC 1 cut(s) 307
BmiI GGNNCC 1 cut(s) 527
BmrFI CCNGG 1 cut(s) 315
BmsI GCATC 1 cut(s) 343
BpiI GAAGAC 1 cut(s) 174
BpmI CTGGAG 1 cut(s) 515
BsaWI WCCGGW 1 cut(s) 496
BsaXI ACNNNNNCTCC 3 cut(s) 27, 454, 484
Bse1I ACTGG 1 cut(s) 532
Bse3DI GCAATG 2 cut(s) 32, 408
BseAI TCCGGA 1 cut(s) 496
BseBI CCWGG 1 cut(s) 315
BseGI GGATG 5 cut(s) 310, 338, 358, 379, 415
BseMI GCAATG 2 cut(s) 32, 408
BseMII CTCAG 2 cut(s) 462, 500
BseNI ACTGG 1 cut(s) 532
BseRI GAGGAG 2 cut(s) 462, 518
BseXI GCAGC 2 cut(s) 404, 493
Bsh1285I CGRYCG 1 cut(s) 247
BshFI GGCC 1 cut(s) 64
BsiEI CGRYCG 1 cut(s) 247
BsiSI CCGG 1 cut(s) 497
BsmAI GTCTC 1 cut(s) 420
BsmI GAATGC 1 cut(s) 440
BsnI GGCC 1 cut(s) 64
Bsp13I TCCGGA 1 cut(s) 496
Bsp143I GATC 1 cut(s) 157
BspACI CCGC 2 cut(s) 198, 247
BspANI GGCC 1 cut(s) 64
BspCNI CTCAG 2 cut(s) 461, 499
BspEI TCCGGA 1 cut(s) 496
BspLI GGNNCC 1 cut(s) 527
BspMAI CTGCAG 1 cut(s) 394
BsrDI GCAATG 2 cut(s) 32, 408
BsrI ACTGG 1 cut(s) 532
BssMI GATC 1 cut(s) 157
Bst2UI CCWGG 1 cut(s) 315
Bst4CI ACNGT 1 cut(s) 386
BstAPI GCANNNNNTGC 1 cut(s) 255
BstC8I GCNNGC 1 cut(s) 260
BstDEI CTNAG 2 cut(s) 448, 486
BstF5I GGATG 5 cut(s) 310, 338, 358, 379, 415
BstHHI GCGC 1 cut(s) 579
BstKTI GATC 1 cut(s) 160
BstMAI GTCTC 1 cut(s) 420
BstMBI GATC 1 cut(s) 157
BstMCI CGRYCG 1 cut(s) 247
BstMWI GCNNNNNNNGC 3 cut(s) 43, 255, 398
BstNI CCWGG 1 cut(s) 315
BstSCI CCNGG 1 cut(s) 313
BstSFI CTRYAG 1 cut(s) 390
BstV1I GCAGC 2 cut(s) 404, 493
BstV2I GAAGAC 1 cut(s) 174
BstXI CCANNNNNNTGG 1 cut(s) 317
BsuRI GGCC 1 cut(s) 64
BtsCI GGATG 5 cut(s) 310, 338, 358, 379, 415
BtsIMutI CAGTG 1 cut(s) 149
Cac8I GCNNGC 1 cut(s) 260
CfoI GCGC 1 cut(s) 579
Cfr13I GGNCC 1 cut(s) 307
Csp6I GTAC 4 cut(s) 387, 415, 466, 490
CviQI GTAC 4 cut(s) 387, 415, 466, 490
DdeI CTNAG 2 cut(s) 448, 486
DpnI GATC 1 cut(s) 159
DpnII GATC 1 cut(s) 157
Eco47I GGWCC 1 cut(s) 307
EcoRII CCWGG 1 cut(s) 313
FaiI YATR 3 cut(s) 285, 420, 585
FauI CCCGC 1 cut(s) 191
FbaI TGATCA 1 cut(s) 157
FblI GTMKAC 3 cut(s) 213, 231, 458
Fnu4HI GCNGC 2 cut(s) 393, 482
FokI GGATG 5 cut(s) 297, 325, 365, 386, 422
Fsp4HI GCNGC 2 cut(s) 393, 482
FspBI CTAG 1 cut(s) 396
GlaI GCGC 1 cut(s) 578
GluI GCNGC 2 cut(s) 393, 482
GsuI CTGGAG 1 cut(s) 515
HaeIII GGCC 1 cut(s) 64
HapII CCGG 1 cut(s) 497
HhaI GCGC 1 cut(s) 579
Hin6I GCGC 1 cut(s) 577
HinP1I GCGC 1 cut(s) 577
HincII GTYRAC 1 cut(s) 459
HindII GTYRAC 1 cut(s) 459
HinfI GANTC 1 cut(s) 135
HpaII CCGG 1 cut(s) 497
HphI GGTGA 4 cut(s) 59, 92, 332, 585
Hpy166II GTNNAC 5 cut(s) 16, 214, 232, 459, 468
Hpy188III TCNNGA 2 cut(s) 155, 497
Hpy8I GTNNAC 5 cut(s) 16, 214, 232, 459, 468
HpyAV CCTTC 1 cut(s) 343
HpyCH4III ACNGT 1 cut(s) 386
HpyCH4IV ACGT 1 cut(s) 464
HpyCH4V TGCA 2 cut(s) 356, 392
HpyF10VI GCNNNNNNNGC 3 cut(s) 43, 255, 398
HpyF3I CTNAG 2 cut(s) 448, 486
HpySE526I ACGT 1 cut(s) 464
HspAI GCGC 1 cut(s) 577
Kpn2I TCCGGA 1 cut(s) 496
Ksp22I TGATCA 1 cut(s) 157
Kzo9I GATC 1 cut(s) 157
LmnI GCTCC 2 cut(s) 531, 584
LpnPI CCDG 8 cut(s) 244, 300, 327, 330, 508, 510, 545, 560
Lsp1109I GCAGC 2 cut(s) 404, 493
LweI GCATC 1 cut(s) 343
MaeI CTAG 1 cut(s) 396
MaeII ACGT 1 cut(s) 464
MaeIII GTNAC 3 cut(s) 187, 289, 380
MalI GATC 1 cut(s) 159
MboI GATC 1 cut(s) 157
MboII GAAGA 1 cut(s) 174
MluCI AATT 3 cut(s) 47, 52, 296
MlyI GAGTC 1 cut(s) 129
MmeI TCCRAC 1 cut(s) 151
MnlI CCTC 9 cut(s) 35, 75, 220, 298, 319, 364, 483, 495, 539
MroI TCCGGA 1 cut(s) 496
MseI TTAA 1 cut(s) 51
MslI CAYNNNNRTG 1 cut(s) 315
MspI CCGG 1 cut(s) 497
MspR9I CCNGG 1 cut(s) 315
Mva1269I GAATGC 1 cut(s) 440
MvaI CCWGG 1 cut(s) 315
MwoI GCNNNNNNNGC 3 cut(s) 43, 255, 398
NdeII GATC 1 cut(s) 157
NlaIV GGNNCC 1 cut(s) 527
NmuCI GTSAC 2 cut(s) 289, 380
PctI GAATGC 1 cut(s) 440
PflFI GACNNNGTC 2 cut(s) 138, 227
PkrI GCNGC 2 cut(s) 394, 483
PleI GAGTC 1 cut(s) 129
PpsI GAGTC 1 cut(s) 129
Psp6I CCWGG 1 cut(s) 313
PspGI CCWGG 1 cut(s) 313
PspN4I GGNNCC 1 cut(s) 527
PspPI GGNCC 1 cut(s) 307
PstI CTGCAG 1 cut(s) 394
PsyI GACNNNGTC 2 cut(s) 138, 227
RsaI GTAC 4 cut(s) 388, 416, 467, 491
RsaNI GTAC 4 cut(s) 387, 415, 466, 490
RseI CAYNNNNRTG 1 cut(s) 315
SalI GTCGAC 1 cut(s) 457
SaqAI TTAA 1 cut(s) 51
SatI GCNGC 2 cut(s) 393, 482
Sau3AI GATC 1 cut(s) 157
Sau96I GGNCC 1 cut(s) 307
ScaI AGTACT 2 cut(s) 388, 416
SchI GAGTC 1 cut(s) 129
ScrFI CCNGG 1 cut(s) 315
SetI ASST 9 cut(s) 96, 105, 178, 213, 231, 282, 330, 397, 467
SfaNI GCATC 1 cut(s) 343
SfcI CTRYAG 1 cut(s) 390
SinI GGWCC 1 cut(s) 307
SmiMI CAYNNNNRTG 1 cut(s) 315
Sse9I AATT 3 cut(s) 47, 52, 296
SsiI CCGC 2 cut(s) 198, 247
SspMI CTAG 1 cut(s) 396
StyD4I CCNGG 1 cut(s) 313
TaaI ACNGT 1 cut(s) 386
TaiI ACGT 1 cut(s) 467
TaqI TCGA 1 cut(s) 458
TasI AATT 3 cut(s) 47, 52, 296
TatI WGTACW 3 cut(s) 386, 414, 489
Tru1I TTAA 1 cut(s) 51
Tru9I TTAA 1 cut(s) 51
TscAI CASTG 1 cut(s) 149
TseFI GTSAC 2 cut(s) 289, 380
TseI GCWGC 2 cut(s) 392, 481
Tsp45I GTSAC 2 cut(s) 289, 380
TspDTI ATGAA 2 cut(s) 433, 505
TspGWI ACGGA 1 cut(s) 147
TspRI CASTG 1 cut(s) 149
Tth111I GACNNNGTC 2 cut(s) 138, 227
VpaK11BI GGWCC 1 cut(s) 307
XapI RAATTY 1 cut(s) 296
XmiI GTMKAC 3 cut(s) 213, 231, 458
XspI CTAG 1 cut(s) 396
ZrmI AGTACT 2 cut(s) 388, 416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.