Rh6AG280200

Cytochrome b5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0013843
Physical Location & Seq
Forward (+)
47493648 .. 47495178
1531 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG280200.1

Sequence Viewer

Length: 618 bp
ATGACATGGGAGTTGGTGTGGACAAAGCCCTCCCACCATTTGCCCCATTCGCAATTTAATTTTGTTTTGGCCTCACCAACCCAACCCAACCCAAACCCACCTTTCTCACCTTTATTATTTTCCCATTGCATTCAAATCCAACACGGACTCTGTCAGTGGAACTCTCTTGATCAGTTTCAAAAAACAGCTCTGTCTTCTGTAACAATGGGCGGGGAGAAAAAGGTCTACACTCTGGACGAGGTCTACAACCACAGCGACCGCAAAGACTGCTGGCTCGTCATTGATGGCAAGGTGTATGATGTGACAAAATTTTTGGAGGACCATCCTGGTGGTGATGAGGTCTTATTGTCATCCACAGGGAAGGATGCAACTGATGATTTTGAGGATGTTGGTCACAGTACTGCAGCTAGAGCAATGATGGATGAGTACTATGTAGGAGACATTGATAAAGCATTCATCTCAGCCAAGTCGACAACGTACACTCCTCCCAAGCAGCCTCAGTACAATCCGGACAAAACAACCGATTTCATCATCAGGCTCCTCCAGTTCCTTGTTCCCCTGCTCATTTTGGGTTTGGCTTTTGTTGTGCGCTCCTATACCAAATCACCAACAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyt-b5

Protein Analysis

205

Amino Acids

23.14

Weight (kDa)

5.52

Isoelectric Point (pI)

25.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 77 - 148 7.2e-29 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013843)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48810
fragaria_vesca FvH4_2g19040
malus_domestica MD05G1175200.v1.1 MD10G1164300.v1.1
prunus_persica Prupe.8G202900_v2.0.a1 Prupe.8G202900_v2.0.a1
pyrus_communis pycom05g16210 pycom10g14170
rosa_chinensis RchiOBHm_Chr6g0284851
rosa_laevigata RLG00000012707
rosa_multiflora Rmu_sc0000686.1_g000017
rosa_roxburghii Rroxscaffold_7G00184300
rosa_rugosa Rorug06G0167500
rosa_samantha Rh6AG280200 Rh6BG280500 Rh6CG282500
rosa_wichuraiana Rw6G024080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 225, 243, 470
AccIII TCCGGA 1 cut(s) 508
AciI CCGC 2 cut(s) 210, 259
AcsI RAATTY 1 cut(s) 308
AfaI GTAC 4 cut(s) 400, 428, 479, 503
AgsI TTSAA 2 cut(s) 134, 179
AjnI CCWGG 1 cut(s) 325
AluBI AGCT 2 cut(s) 188, 407
AluI AGCT 2 cut(s) 188, 407
Alw26I GTCTC 1 cut(s) 432
Aor13HI TCCGGA 1 cut(s) 508
AoxI GGCC 1 cut(s) 69
ApeKI GCWGC 2 cut(s) 404, 493
ApoI RAATTY 1 cut(s) 308
ArsI GACNNNNNNTTYG 2 cut(s) 295, 327
AspLEI GCGC 1 cut(s) 591
AspS9I GGNCC 1 cut(s) 319
AsuHPI GGTGA 4 cut(s) 66, 99, 344, 597
AvaII GGWCC 1 cut(s) 319
BbsI GAAGAC 1 cut(s) 186
BbvI GCAGC 2 cut(s) 416, 505
BccI CCATC 3 cut(s) 278, 330, 412
BciT130I CCWGG 1 cut(s) 327
BclI TGATCA 1 cut(s) 169
BcoDI GTCTC 1 cut(s) 432
BfaI CTAG 1 cut(s) 408
BfmI CTRYAG 1 cut(s) 402
BisI GCNGC 2 cut(s) 405, 494
BlsI GCNGC 2 cut(s) 406, 495
BmcAI AGTACT 2 cut(s) 400, 428
Bme1390I CCNGG 1 cut(s) 327
Bme18I GGWCC 1 cut(s) 319
BmgT120I GGNCC 1 cut(s) 319
BmiI GGNNCC 1 cut(s) 539
BmrFI CCNGG 1 cut(s) 327
BmsI GCATC 1 cut(s) 355
BpiI GAAGAC 1 cut(s) 186
BpmI CTGGAG 1 cut(s) 527
BsaWI WCCGGW 1 cut(s) 508
BsaXI ACNNNNNCTCC 3 cut(s) 32, 466, 496
Bse1I ACTGG 1 cut(s) 544
Bse3DI GCAATG 2 cut(s) 124, 420
BseAI TCCGGA 1 cut(s) 508
BseBI CCWGG 1 cut(s) 327
BseGI GGATG 5 cut(s) 322, 350, 370, 391, 427
BseMI GCAATG 2 cut(s) 124, 420
BseMII CTCAG 2 cut(s) 474, 512
BseNI ACTGG 1 cut(s) 544
BseRI GAGGAG 2 cut(s) 474, 530
BseXI GCAGC 2 cut(s) 416, 505
Bsh1285I CGRYCG 1 cut(s) 259
BshFI GGCC 1 cut(s) 71
BsiEI CGRYCG 1 cut(s) 259
BsiSI CCGG 1 cut(s) 509
BsmAI GTCTC 1 cut(s) 432
BsmI GAATGC 2 cut(s) 129, 452
BsnI GGCC 1 cut(s) 71
Bsp13I TCCGGA 1 cut(s) 508
Bsp143I GATC 1 cut(s) 169
BspACI CCGC 2 cut(s) 210, 259
BspANI GGCC 1 cut(s) 71
BspCNI CTCAG 2 cut(s) 473, 511
BspEI TCCGGA 1 cut(s) 508
BspLI GGNNCC 1 cut(s) 539
BspMAI CTGCAG 1 cut(s) 406
BsrDI GCAATG 2 cut(s) 124, 420
BsrI ACTGG 1 cut(s) 544
BssMI GATC 1 cut(s) 169
Bst2UI CCWGG 1 cut(s) 327
Bst4CI ACNGT 1 cut(s) 398
BstAPI GCANNNNNTGC 1 cut(s) 267
BstC8I GCNNGC 1 cut(s) 272
BstDEI CTNAG 2 cut(s) 460, 498
BstF5I GGATG 5 cut(s) 322, 350, 370, 391, 427
BstHHI GCGC 1 cut(s) 591
BstKTI GATC 1 cut(s) 172
BstMAI GTCTC 1 cut(s) 432
BstMBI GATC 1 cut(s) 169
BstMCI CGRYCG 1 cut(s) 259
BstMWI GCNNNNNNNGC 3 cut(s) 49, 267, 410
BstNI CCWGG 1 cut(s) 327
BstSCI CCNGG 1 cut(s) 325
BstSFI CTRYAG 1 cut(s) 402
BstV1I GCAGC 2 cut(s) 416, 505
BstV2I GAAGAC 1 cut(s) 186
BstXI CCANNNNNNTGG 1 cut(s) 329
BsuRI GGCC 1 cut(s) 71
BtsCI GGATG 5 cut(s) 322, 350, 370, 391, 427
BtsIMutI CAGTG 1 cut(s) 161
Cac8I GCNNGC 1 cut(s) 272
CfoI GCGC 1 cut(s) 591
Cfr13I GGNCC 1 cut(s) 319
Csp6I GTAC 4 cut(s) 399, 427, 478, 502
CviAII CATG 1 cut(s) 6
CviQI GTAC 4 cut(s) 399, 427, 478, 502
DdeI CTNAG 2 cut(s) 460, 498
DpnI GATC 1 cut(s) 171
DpnII GATC 1 cut(s) 169
Eco47I GGWCC 1 cut(s) 319
EcoRII CCWGG 1 cut(s) 325
FaeI CATG 1 cut(s) 9
FaiI YATR 4 cut(s) 7, 297, 432, 597
FatI CATG 1 cut(s) 5
FauI CCCGC 1 cut(s) 203
FbaI TGATCA 1 cut(s) 169
FblI GTMKAC 3 cut(s) 225, 243, 470
Fnu4HI GCNGC 2 cut(s) 405, 494
FokI GGATG 5 cut(s) 309, 337, 377, 398, 434
Fsp4HI GCNGC 2 cut(s) 405, 494
FspBI CTAG 1 cut(s) 408
GlaI GCGC 1 cut(s) 590
GluI GCNGC 2 cut(s) 405, 494
GsuI CTGGAG 1 cut(s) 527
HaeIII GGCC 1 cut(s) 71
HapII CCGG 1 cut(s) 509
HhaI GCGC 1 cut(s) 591
Hin1II CATG 1 cut(s) 9
Hin6I GCGC 1 cut(s) 589
HinP1I GCGC 1 cut(s) 589
HincII GTYRAC 1 cut(s) 471
HindII GTYRAC 1 cut(s) 471
HinfI GANTC 1 cut(s) 147
HpaII CCGG 1 cut(s) 509
HphI GGTGA 4 cut(s) 66, 99, 344, 597
Hpy166II GTNNAC 5 cut(s) 21, 226, 244, 471, 480
Hpy188III TCNNGA 3 cut(s) 167, 233, 509
Hpy8I GTNNAC 5 cut(s) 21, 226, 244, 471, 480
HpyAV CCTTC 1 cut(s) 355
HpyCH4III ACNGT 1 cut(s) 398
HpyCH4IV ACGT 1 cut(s) 476
HpyCH4V TGCA 3 cut(s) 129, 368, 404
HpyF10VI GCNNNNNNNGC 3 cut(s) 49, 267, 410
HpyF3I CTNAG 2 cut(s) 460, 498
HpySE526I ACGT 1 cut(s) 476
Hsp92II CATG 1 cut(s) 9
HspAI GCGC 1 cut(s) 589
Kpn2I TCCGGA 1 cut(s) 508
Ksp22I TGATCA 1 cut(s) 169
Kzo9I GATC 1 cut(s) 169
LmnI GCTCC 2 cut(s) 543, 596
LpnPI CCDG 9 cut(s) 218, 256, 312, 339, 342, 520, 522, 557, 572
Lsp1109I GCAGC 2 cut(s) 416, 505
LweI GCATC 1 cut(s) 355
MaeI CTAG 1 cut(s) 408
MaeII ACGT 1 cut(s) 476
MaeIII GTNAC 3 cut(s) 199, 301, 392
MalI GATC 1 cut(s) 171
MboI GATC 1 cut(s) 169
MboII GAAGA 1 cut(s) 186
MluCI AATT 3 cut(s) 53, 58, 308
MlyI GAGTC 1 cut(s) 141
MmeI TCCRAC 1 cut(s) 163
MnlI CCTC 9 cut(s) 40, 82, 232, 310, 331, 376, 495, 507, 551
MroI TCCGGA 1 cut(s) 508
MseI TTAA 1 cut(s) 57
MslI CAYNNNNRTG 1 cut(s) 327
MspI CCGG 1 cut(s) 509
MspR9I CCNGG 1 cut(s) 327
Mva1269I GAATGC 2 cut(s) 129, 452
MvaI CCWGG 1 cut(s) 327
MwoI GCNNNNNNNGC 3 cut(s) 49, 267, 410
NdeII GATC 1 cut(s) 169
NlaIII CATG 1 cut(s) 9
NlaIV GGNNCC 1 cut(s) 539
NmuCI GTSAC 2 cut(s) 301, 392
PctI GAATGC 2 cut(s) 129, 452
PflFI GACNNNGTC 2 cut(s) 150, 239
PkrI GCNGC 2 cut(s) 406, 495
PleI GAGTC 1 cut(s) 141
PpsI GAGTC 1 cut(s) 141
Psp6I CCWGG 1 cut(s) 325
PspGI CCWGG 1 cut(s) 325
PspN4I GGNNCC 1 cut(s) 539
PspPI GGNCC 1 cut(s) 319
PstI CTGCAG 1 cut(s) 406
PsyI GACNNNGTC 2 cut(s) 150, 239
RsaI GTAC 4 cut(s) 400, 428, 479, 503
RsaNI GTAC 4 cut(s) 399, 427, 478, 502
RseI CAYNNNNRTG 1 cut(s) 327
SalI GTCGAC 1 cut(s) 469
SaqAI TTAA 1 cut(s) 57
SatI GCNGC 2 cut(s) 405, 494
Sau3AI GATC 1 cut(s) 169
Sau96I GGNCC 1 cut(s) 319
ScaI AGTACT 2 cut(s) 400, 428
SchI GAGTC 1 cut(s) 141
ScrFI CCNGG 1 cut(s) 327
SetI ASST 9 cut(s) 103, 112, 190, 225, 243, 294, 342, 409, 479
SfaNI GCATC 1 cut(s) 355
SfcI CTRYAG 1 cut(s) 402
SinI GGWCC 1 cut(s) 319
SmiMI CAYNNNNRTG 1 cut(s) 327
Sse9I AATT 3 cut(s) 53, 58, 308
SsiI CCGC 2 cut(s) 210, 259
SspMI CTAG 1 cut(s) 408
StyD4I CCNGG 1 cut(s) 325
TaaI ACNGT 1 cut(s) 398
TaiI ACGT 1 cut(s) 479
TaqI TCGA 1 cut(s) 470
TasI AATT 3 cut(s) 53, 58, 308
TatI WGTACW 3 cut(s) 398, 426, 501
Tru1I TTAA 1 cut(s) 57
Tru9I TTAA 1 cut(s) 57
TscAI CASTG 1 cut(s) 161
TseFI GTSAC 2 cut(s) 301, 392
TseI GCWGC 2 cut(s) 404, 493
Tsp45I GTSAC 2 cut(s) 301, 392
TspDTI ATGAA 2 cut(s) 445, 517
TspGWI ACGGA 1 cut(s) 159
TspRI CASTG 1 cut(s) 161
Tth111I GACNNNGTC 2 cut(s) 150, 239
VpaK11BI GGWCC 1 cut(s) 319
XapI RAATTY 1 cut(s) 308
XmiI GTMKAC 3 cut(s) 225, 243, 470
XspI CTAG 1 cut(s) 408
ZrmI AGTACT 2 cut(s) 400, 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.