FvH4_2g19040

Cytochrome b5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0013843
Physical Location & Seq
Forward (+)
16218045 .. 16219937
1893 bp
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UTR
Exon/CDS
Intron
FvH4_2g19040.t1

Sequence Viewer

Length: 414 bp
ATGGGCGCCGAGAAAAAGGTCTATACTTTGGAAGTGGTCTCCAAGCACAGTGACCGCAAAGACTGCTGGCTCGTCATTGATGGCAAGGTGTATGATGTGACAAAATTCTTGGAGGACCATCCTGGTGGTGATGAGGTCTTATTGTCATCCACAGGGAAGGATGCAACTGATGATTTTGACGATGTTGGTCACAGTACCTCTGCTAGAGCAATGATGGATGAATACTATGTAGGTGACTTTGATAAAGCATTCATCCCTGCTAAGACGGCAAAGTACACTCCTCCCAAGCAGCCTCATTACAATCCTGATAAAACATCCGATTTCATCATCAGACTCCTCCAGTTCCTTGTTCCCCTGCTCATTTTGGGTCTGGCTTTCGTTGTGCGCGCCTATACCAAATCACCGACAACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyt-b5

Protein Analysis

138

Amino Acids

15.37

Weight (kDa)

5.49

Isoelectric Point (pI)

24.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 9 - 80 5.8e-29 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013843)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48810
fragaria_vesca FvH4_2g19040
malus_domestica MD05G1175200.v1.1 MD10G1164300.v1.1
prunus_persica Prupe.8G202900_v2.0.a1 Prupe.8G202900_v2.0.a1
pyrus_communis pycom05g16210 pycom10g14170
rosa_chinensis RchiOBHm_Chr6g0284851
rosa_laevigata RLG00000012707
rosa_multiflora Rmu_sc0000686.1_g000017
rosa_roxburghii Rroxscaffold_7G00184300
rosa_rugosa Rorug06G0167500
rosa_samantha Rh6AG280200 Rh6BG280500 Rh6CG282500
rosa_wichuraiana Rw6G024080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 5
AccII CGCG 1 cut(s) 387
AciI CCGC 1 cut(s) 55
AcsI RAATTY 1 cut(s) 104
AcyI GRCGYC 1 cut(s) 6
AfaI GTAC 2 cut(s) 196, 275
AjnI CCWGG 1 cut(s) 121
Alw26I GTCTC 1 cut(s) 43
ApeKI GCWGC 1 cut(s) 289
ApoI RAATTY 1 cut(s) 104
AspLEI GCGC 3 cut(s) 8, 387, 389
AspS9I GGNCC 1 cut(s) 115
AsuHPI GGTGA 3 cut(s) 140, 245, 393
AvaII GGWCC 1 cut(s) 115
BanI GGYRCC 1 cut(s) 5
BbvI GCAGC 1 cut(s) 301
BccI CCATC 3 cut(s) 74, 126, 208
BceAI ACGGC 1 cut(s) 282
BciT130I CCWGG 1 cut(s) 123
BcoDI GTCTC 1 cut(s) 43
BfaI CTAG 1 cut(s) 204
BfoI RGCGCY 1 cut(s) 9
BisI GCNGC 1 cut(s) 290
BlsI GCNGC 1 cut(s) 291
Bme1390I CCNGG 1 cut(s) 123
Bme18I GGWCC 1 cut(s) 115
BmgT120I GGNCC 1 cut(s) 115
BmiI GGNNCC 1 cut(s) 7
BmrFI CCNGG 1 cut(s) 123
BmsI GCATC 1 cut(s) 151
BpmI CTGGAG 1 cut(s) 323
BsaHI GRCGYC 1 cut(s) 6
BsaI GGTCTC 1 cut(s) 43
Bse1I ACTGG 1 cut(s) 340
Bse3DI GCAATG 1 cut(s) 216
BseBI CCWGG 1 cut(s) 123
BseGI GGATG 6 cut(s) 118, 146, 166, 223, 252, 314
BseMI GCAATG 1 cut(s) 216
BseNI ACTGG 1 cut(s) 340
BsePI GCGCGC 1 cut(s) 385
BseRI GAGGAG 2 cut(s) 270, 326
BseXI GCAGC 1 cut(s) 301
Bsh1236I CGCG 1 cut(s) 387
BshNI GGYRCC 1 cut(s) 5
BsmAI GTCTC 1 cut(s) 43
BsmI GAATGC 1 cut(s) 248
Bso31I GGTCTC 1 cut(s) 43
BspACI CCGC 1 cut(s) 55
BspFNI CGCG 1 cut(s) 387
BspLI GGNNCC 1 cut(s) 7
BspT107I GGYRCC 1 cut(s) 5
BspTNI GGTCTC 1 cut(s) 43
BsrDI GCAATG 1 cut(s) 216
BsrI ACTGG 1 cut(s) 340
BssHII GCGCGC 1 cut(s) 385
BssNI GRCGYC 1 cut(s) 6
Bst2UI CCWGG 1 cut(s) 123
Bst4CI ACNGT 2 cut(s) 50, 194
BstACI GRCGYC 1 cut(s) 6
BstAPI GCANNNNNTGC 1 cut(s) 63
BstC8I GCNNGC 2 cut(s) 68, 387
BstDEI CTNAG 1 cut(s) 261
BstF5I GGATG 6 cut(s) 118, 146, 166, 223, 252, 314
BstFNI CGCG 1 cut(s) 387
BstH2I RGCGCY 1 cut(s) 9
BstHHI GCGC 3 cut(s) 8, 387, 389
BstMAI GTCTC 1 cut(s) 43
BstMWI GCNNNNNNNGC 2 cut(s) 63, 266
BstNI CCWGG 1 cut(s) 123
BstSCI CCNGG 1 cut(s) 121
BstUI CGCG 1 cut(s) 387
BstV1I GCAGC 1 cut(s) 301
BstXI CCANNNNNNTGG 1 cut(s) 125
BtsCI GGATG 6 cut(s) 118, 146, 166, 223, 252, 314
BtsIMutI CAGTG 1 cut(s) 55
Cac8I GCNNGC 2 cut(s) 68, 387
CfoI GCGC 3 cut(s) 8, 387, 389
Cfr13I GGNCC 1 cut(s) 115
Csp6I GTAC 2 cut(s) 195, 274
CviJI RGCY 3 cut(s) 70, 292, 374
CviKI_1 RGCY 3 cut(s) 70, 292, 374
CviQI GTAC 2 cut(s) 195, 274
DdeI CTNAG 1 cut(s) 261
DinI GGCGCC 1 cut(s) 7
Eco31I GGTCTC 1 cut(s) 43
Eco47I GGWCC 1 cut(s) 115
EcoRII CCWGG 1 cut(s) 121
EgeI GGCGCC 1 cut(s) 7
EheI GGCGCC 1 cut(s) 7
FaiI YATR 4 cut(s) 24, 93, 228, 393
Fnu4HI GCNGC 1 cut(s) 290
FokI GGATG 6 cut(s) 105, 133, 173, 230, 239, 301
Fsp4HI GCNGC 1 cut(s) 290
FspBI CTAG 1 cut(s) 204
GlaI GCGC 3 cut(s) 7, 386, 388
GluI GCNGC 1 cut(s) 290
GsuI CTGGAG 1 cut(s) 323
HaeII RGCGCY 1 cut(s) 9
HhaI GCGC 3 cut(s) 8, 387, 389
Hin1I GRCGYC 1 cut(s) 6
Hin6I GCGC 3 cut(s) 6, 385, 387
HinP1I GCGC 3 cut(s) 6, 385, 387
HinfI GANTC 1 cut(s) 333
HphI GGTGA 3 cut(s) 140, 245, 393
Hpy166II GTNNAC 1 cut(s) 276
Hpy188I TCNGA 2 cut(s) 319, 332
Hpy188III TCNNGA 1 cut(s) 305
Hpy8I GTNNAC 1 cut(s) 276
HpyAV CCTTC 1 cut(s) 151
HpyCH4III ACNGT 2 cut(s) 50, 194
HpyCH4V TGCA 1 cut(s) 164
HpyF10VI GCNNNNNNNGC 2 cut(s) 63, 266
HpyF3I CTNAG 1 cut(s) 261
Hsp92I GRCGYC 1 cut(s) 6
HspAI GCGC 3 cut(s) 6, 385, 387
KasI GGCGCC 1 cut(s) 5
LpnPI CCDG 9 cut(s) 52, 108, 135, 138, 270, 318, 353, 356, 368
Lsp1109I GCAGC 1 cut(s) 301
LweI GCATC 1 cut(s) 151
MaeI CTAG 1 cut(s) 204
MaeIII GTNAC 4 cut(s) 50, 97, 188, 233
MluCI AATT 1 cut(s) 104
Mly113I GGCGCC 1 cut(s) 6
MlyI GAGTC 1 cut(s) 327
MnlI CCTC 6 cut(s) 106, 127, 208, 291, 303, 347
MslI CAYNNNNRTG 1 cut(s) 123
MspR9I CCNGG 1 cut(s) 123
Mva1269I GAATGC 1 cut(s) 248
MvaI CCWGG 1 cut(s) 123
MvnI CGCG 1 cut(s) 387
MwoI GCNNNNNNNGC 2 cut(s) 63, 266
NarI GGCGCC 1 cut(s) 6
NlaIV GGNNCC 1 cut(s) 7
NmeAIII GCCGAG 1 cut(s) 34
NmuCI GTSAC 4 cut(s) 50, 97, 188, 233
PauI GCGCGC 1 cut(s) 385
PctI GAATGC 1 cut(s) 248
PkrI GCNGC 1 cut(s) 291
PleI GAGTC 1 cut(s) 327
PluTI GGCGCC 1 cut(s) 9
PpsI GAGTC 1 cut(s) 327
Psp6I CCWGG 1 cut(s) 121
PspGI CCWGG 1 cut(s) 121
PspN4I GGNNCC 1 cut(s) 7
PspPI GGNCC 1 cut(s) 115
PteI GCGCGC 1 cut(s) 385
RsaI GTAC 2 cut(s) 196, 275
RsaNI GTAC 2 cut(s) 195, 274
RseI CAYNNNNRTG 1 cut(s) 123
SatI GCNGC 1 cut(s) 290
Sau96I GGNCC 1 cut(s) 115
SchI GAGTC 1 cut(s) 327
ScrFI CCNGG 1 cut(s) 123
SetI ASST 6 cut(s) 21, 90, 138, 200, 235, 413
SfaNI GCATC 1 cut(s) 151
SfoI GGCGCC 1 cut(s) 7
SinI GGWCC 1 cut(s) 115
SmiMI CAYNNNNRTG 1 cut(s) 123
Sse9I AATT 1 cut(s) 104
SsiI CCGC 1 cut(s) 55
SspDI GGCGCC 1 cut(s) 5
SspMI CTAG 1 cut(s) 204
StyD4I CCNGG 1 cut(s) 121
TaaI ACNGT 2 cut(s) 50, 194
TasI AATT 1 cut(s) 104
TatI WGTACW 1 cut(s) 273
TscAI CASTG 1 cut(s) 55
TseFI GTSAC 4 cut(s) 50, 97, 188, 233
TseI GCWGC 1 cut(s) 289
Tsp45I GTSAC 4 cut(s) 50, 97, 188, 233
TspDTI ATGAA 3 cut(s) 234, 241, 313
TspRI CASTG 1 cut(s) 55
VpaK11BI GGWCC 1 cut(s) 115
XapI RAATTY 1 cut(s) 104
XspI CTAG 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.