RchiOBHm_Chr6g0284851

Cytochrome b5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0013843
Physical Location & Seq
Forward (+)
48169779 .. 48171492
1714 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25547

Sequence Viewer

Length: 609 bp
ATGACATGGGGGTTGGTGTGGACAAAGCCCTCCCACCATTGCCCCATTCGCAATTTAATTTTGTTTGCCCTCAACAACCCAACCCAAACCCACCTTTCTTCTCACCTTTATTATTTTCCCATTCAAATCCAACACGGACTCTGTCAGTGGAACTCTCTTGATCAGTTTCAAAAAACAGCTCTGTCTTCTGTAACAATGGGCGGGGAGAAAAAGGTCTACACTTTGGACGAGGTCTACAAGCACAGCGACCGCAAAGACTGCTGGCTCGTCATTGATGGCAAGGTGTATGATGTGACAAAATTTTTGGAGGACCATCCTGGTGGTGATGAGGTCTTATTGTCATCCACAGGGAAGGATGCAACTGATGATTTTGAGGATGTTGGTCACAGTACTGCAGCTAGAGCAATGATGGATGAGTACTATGTAGGAGACATTGATAAAGCATTCATCTCAGCCAAGTCGACAGAGTACACTCCTCCCAAGCAGCCTCAGTACAATCCGGACAAAACATCCGATTTCATCATCAGACTCCTCCAGTTCCTTGTTCCCCTGCTCATTTTGGGTTTGGCTTTTGTTGTGCGCTCCTATACCAAATCACCAACAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyt-b5

Protein Analysis

202

Amino Acids

22.94

Weight (kDa)

5.86

Isoelectric Point (pI)

23.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 74 - 145 2.4e-29 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013843)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48810
fragaria_vesca FvH4_2g19040
malus_domestica MD05G1175200.v1.1 MD10G1164300.v1.1
prunus_persica Prupe.8G202900_v2.0.a1 Prupe.8G202900_v2.0.a1
pyrus_communis pycom05g16210 pycom10g14170
rosa_chinensis RchiOBHm_Chr6g0284851
rosa_laevigata RLG00000012707
rosa_multiflora Rmu_sc0000686.1_g000017
rosa_roxburghii Rroxscaffold_7G00184300
rosa_rugosa Rorug06G0167500
rosa_samantha Rh6AG280200 Rh6BG280500 Rh6CG282500
rosa_wichuraiana Rw6G024080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 216, 234, 461
AccIII TCCGGA 1 cut(s) 499
AciI CCGC 2 cut(s) 201, 250
AcsI RAATTY 1 cut(s) 299
AfaI GTAC 4 cut(s) 391, 419, 470, 494
AgsI TTSAA 2 cut(s) 125, 170
AjnI CCWGG 1 cut(s) 316
AluBI AGCT 2 cut(s) 179, 398
AluI AGCT 2 cut(s) 179, 398
Alw26I GTCTC 1 cut(s) 423
Aor13HI TCCGGA 1 cut(s) 499
ApeKI GCWGC 2 cut(s) 395, 484
ApoI RAATTY 1 cut(s) 299
ArsI GACNNNNNNTTYG 2 cut(s) 286, 318
AspLEI GCGC 1 cut(s) 582
AspS9I GGNCC 1 cut(s) 310
AsuHPI GGTGA 3 cut(s) 95, 335, 588
AvaII GGWCC 1 cut(s) 310
BbsI GAAGAC 1 cut(s) 177
BbvI GCAGC 2 cut(s) 407, 496
BccI CCATC 3 cut(s) 269, 321, 403
BciT130I CCWGG 1 cut(s) 318
BclI TGATCA 1 cut(s) 160
BcoDI GTCTC 1 cut(s) 423
BfaI CTAG 1 cut(s) 399
BfmI CTRYAG 1 cut(s) 393
BisI GCNGC 2 cut(s) 396, 485
BlsI GCNGC 2 cut(s) 397, 486
BmcAI AGTACT 2 cut(s) 391, 419
Bme1390I CCNGG 1 cut(s) 318
Bme18I GGWCC 1 cut(s) 310
BmgT120I GGNCC 1 cut(s) 310
BmrFI CCNGG 1 cut(s) 318
BmsI GCATC 1 cut(s) 346
BpiI GAAGAC 1 cut(s) 177
BpmI CTGGAG 1 cut(s) 518
BsaWI WCCGGW 1 cut(s) 499
Bse1I ACTGG 1 cut(s) 535
Bse3DI GCAATG 2 cut(s) 37, 411
BseAI TCCGGA 1 cut(s) 499
BseBI CCWGG 1 cut(s) 318
BseGI GGATG 6 cut(s) 313, 341, 361, 382, 418, 509
BseMI GCAATG 2 cut(s) 37, 411
BseMII CTCAG 2 cut(s) 465, 503
BseNI ACTGG 1 cut(s) 535
BseRI GAGGAG 2 cut(s) 465, 521
BseXI GCAGC 2 cut(s) 407, 496
Bsh1285I CGRYCG 1 cut(s) 250
BsiEI CGRYCG 1 cut(s) 250
BsiSI CCGG 1 cut(s) 500
BsmAI GTCTC 1 cut(s) 423
BsmI GAATGC 1 cut(s) 443
Bsp13I TCCGGA 1 cut(s) 499
Bsp143I GATC 1 cut(s) 160
BspACI CCGC 2 cut(s) 201, 250
BspCNI CTCAG 2 cut(s) 464, 502
BspEI TCCGGA 1 cut(s) 499
BspMAI CTGCAG 1 cut(s) 397
BsrDI GCAATG 2 cut(s) 37, 411
BsrI ACTGG 1 cut(s) 535
BssMI GATC 1 cut(s) 160
Bst2UI CCWGG 1 cut(s) 318
Bst4CI ACNGT 1 cut(s) 389
BstAPI GCANNNNNTGC 1 cut(s) 258
BstC8I GCNNGC 1 cut(s) 263
BstDEI CTNAG 2 cut(s) 451, 489
BstF5I GGATG 6 cut(s) 313, 341, 361, 382, 418, 509
BstHHI GCGC 1 cut(s) 582
BstKTI GATC 1 cut(s) 163
BstMAI GTCTC 1 cut(s) 423
BstMBI GATC 1 cut(s) 160
BstMCI CGRYCG 1 cut(s) 250
BstMWI GCNNNNNNNGC 3 cut(s) 48, 258, 401
BstNI CCWGG 1 cut(s) 318
BstSCI CCNGG 1 cut(s) 316
BstSFI CTRYAG 1 cut(s) 393
BstV1I GCAGC 2 cut(s) 407, 496
BstV2I GAAGAC 1 cut(s) 177
BstXI CCANNNNNNTGG 1 cut(s) 320
BtsCI GGATG 6 cut(s) 313, 341, 361, 382, 418, 509
BtsIMutI CAGTG 1 cut(s) 152
Cac8I GCNNGC 1 cut(s) 263
CfoI GCGC 1 cut(s) 582
Cfr13I GGNCC 1 cut(s) 310
Csp6I GTAC 4 cut(s) 390, 418, 469, 493
CviAII CATG 1 cut(s) 6
CviJI RGCY 8 cut(s) 28, 179, 265, 398, 455, 487, 569, 605
CviKI_1 RGCY 8 cut(s) 28, 179, 265, 398, 455, 487, 569, 605
CviQI GTAC 4 cut(s) 390, 418, 469, 493
DdeI CTNAG 2 cut(s) 451, 489
DpnI GATC 1 cut(s) 162
DpnII GATC 1 cut(s) 160
Eco47I GGWCC 1 cut(s) 310
EcoRII CCWGG 1 cut(s) 316
FaeI CATG 1 cut(s) 9
FaiI YATR 4 cut(s) 7, 288, 423, 588
FatI CATG 1 cut(s) 5
FauI CCCGC 1 cut(s) 194
FbaI TGATCA 1 cut(s) 160
FblI GTMKAC 3 cut(s) 216, 234, 461
Fnu4HI GCNGC 2 cut(s) 396, 485
FokI GGATG 6 cut(s) 300, 328, 368, 389, 425, 496
Fsp4HI GCNGC 2 cut(s) 396, 485
FspBI CTAG 1 cut(s) 399
GlaI GCGC 1 cut(s) 581
GluI GCNGC 2 cut(s) 396, 485
GsuI CTGGAG 1 cut(s) 518
HapII CCGG 1 cut(s) 500
HhaI GCGC 1 cut(s) 582
Hin1II CATG 1 cut(s) 9
Hin6I GCGC 1 cut(s) 580
HinP1I GCGC 1 cut(s) 580
HincII GTYRAC 1 cut(s) 462
HindII GTYRAC 1 cut(s) 462
HinfI GANTC 2 cut(s) 138, 528
HpaII CCGG 1 cut(s) 500
HphI GGTGA 3 cut(s) 95, 335, 588
Hpy166II GTNNAC 5 cut(s) 21, 217, 235, 462, 471
Hpy188I TCNGA 2 cut(s) 514, 527
Hpy188III TCNNGA 2 cut(s) 158, 500
Hpy8I GTNNAC 5 cut(s) 21, 217, 235, 462, 471
HpyAV CCTTC 1 cut(s) 346
HpyCH4III ACNGT 1 cut(s) 389
HpyCH4V TGCA 2 cut(s) 359, 395
HpyF10VI GCNNNNNNNGC 3 cut(s) 48, 258, 401
HpyF3I CTNAG 2 cut(s) 451, 489
Hsp92II CATG 1 cut(s) 9
HspAI GCGC 1 cut(s) 580
Kpn2I TCCGGA 1 cut(s) 499
Ksp22I TGATCA 1 cut(s) 160
Kzo9I GATC 1 cut(s) 160
LmnI GCTCC 1 cut(s) 587
LpnPI CCDG 7 cut(s) 247, 303, 330, 333, 513, 548, 563
Lsp1109I GCAGC 2 cut(s) 407, 496
LweI GCATC 1 cut(s) 346
MaeI CTAG 1 cut(s) 399
MaeIII GTNAC 3 cut(s) 190, 292, 383
MalI GATC 1 cut(s) 162
MboI GATC 1 cut(s) 160
MboII GAAGA 2 cut(s) 90, 177
MluCI AATT 3 cut(s) 52, 57, 299
MlyI GAGTC 2 cut(s) 132, 522
MmeI TCCRAC 1 cut(s) 154
MnlI CCTC 9 cut(s) 40, 80, 223, 301, 322, 367, 486, 498, 542
MroI TCCGGA 1 cut(s) 499
MseI TTAA 1 cut(s) 56
MslI CAYNNNNRTG 1 cut(s) 318
MspI CCGG 1 cut(s) 500
MspR9I CCNGG 1 cut(s) 318
Mva1269I GAATGC 1 cut(s) 443
MvaI CCWGG 1 cut(s) 318
MwoI GCNNNNNNNGC 3 cut(s) 48, 258, 401
NdeII GATC 1 cut(s) 160
NlaIII CATG 1 cut(s) 9
NmuCI GTSAC 2 cut(s) 292, 383
PctI GAATGC 1 cut(s) 443
PflFI GACNNNGTC 2 cut(s) 141, 230
PkrI GCNGC 2 cut(s) 397, 486
PleI GAGTC 2 cut(s) 132, 522
PpsI GAGTC 2 cut(s) 132, 522
Psp6I CCWGG 1 cut(s) 316
PspGI CCWGG 1 cut(s) 316
PspPI GGNCC 1 cut(s) 310
PstI CTGCAG 1 cut(s) 397
PsyI GACNNNGTC 2 cut(s) 141, 230
RsaI GTAC 4 cut(s) 391, 419, 470, 494
RsaNI GTAC 4 cut(s) 390, 418, 469, 493
RseI CAYNNNNRTG 1 cut(s) 318
SalI GTCGAC 1 cut(s) 460
SaqAI TTAA 1 cut(s) 56
SatI GCNGC 2 cut(s) 396, 485
Sau3AI GATC 1 cut(s) 160
Sau96I GGNCC 1 cut(s) 310
ScaI AGTACT 2 cut(s) 391, 419
SchI GAGTC 2 cut(s) 132, 522
ScrFI CCNGG 1 cut(s) 318
SetI ASST 8 cut(s) 96, 108, 181, 216, 234, 285, 333, 400
SfaNI GCATC 1 cut(s) 346
SfcI CTRYAG 1 cut(s) 393
SinI GGWCC 1 cut(s) 310
SmiMI CAYNNNNRTG 1 cut(s) 318
Sse9I AATT 3 cut(s) 52, 57, 299
SsiI CCGC 2 cut(s) 201, 250
SspMI CTAG 1 cut(s) 399
StyD4I CCNGG 1 cut(s) 316
TaaI ACNGT 1 cut(s) 389
TaqI TCGA 1 cut(s) 461
TasI AATT 3 cut(s) 52, 57, 299
TatI WGTACW 4 cut(s) 389, 417, 468, 492
Tru1I TTAA 1 cut(s) 56
Tru9I TTAA 1 cut(s) 56
TscAI CASTG 1 cut(s) 152
TseFI GTSAC 2 cut(s) 292, 383
TseI GCWGC 2 cut(s) 395, 484
Tsp45I GTSAC 2 cut(s) 292, 383
TspDTI ATGAA 2 cut(s) 436, 508
TspGWI ACGGA 1 cut(s) 150
TspRI CASTG 1 cut(s) 152
Tth111I GACNNNGTC 2 cut(s) 141, 230
VpaK11BI GGWCC 1 cut(s) 310
XapI RAATTY 1 cut(s) 299
XmiI GTMKAC 3 cut(s) 216, 234, 461
XspI CTAG 1 cut(s) 399
ZrmI AGTACT 2 cut(s) 391, 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.