MD05G1175200.v1.1

Cytochrome b5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0013843
Physical Location & Seq
Forward (+)
30300451 .. 30303119
2669 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1175200.v1.1.491

Sequence Viewer

Length: 411 bp
ATGGCTGGAGACAAAAAGGTTTATACTTTGGCTGAGGTCTCATCTCACAGTGATCGCAAGGACTGCTGGCTTGTCATCGATGGAAAGGTGTATGATGTAACAAAATTCTTGGAGGACCATCCTGGTGGTGATGAGGTCTTATTGGCAGCCACAGGAAAGGACGCAAGTAATGATTTCGAAGATGTTGGTCACAGTTCCACTGCTAGAGCAATGATGGACGAATTTTACGTTGGAGACATTAATTCAGCATCCATCCCCGCAAAGAGGATGTACACTCCTCCCAAGCAGCCTCACTACAACCAGGACAAGACAACTGATTTCGTCATCAAACTCCTCCAGTTCATTGTTCCTCTGCTAATTTTGGGCTTAGCCTTTGGCGTCCGCTTCTACACCAAATCATCAACGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyt-b5

Protein Analysis

137

Amino Acids

15.08

Weight (kDa)

5.47

Isoelectric Point (pI)

33.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 9 - 80 9.9e-29 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013843)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48810
fragaria_vesca FvH4_2g19040
malus_domestica MD05G1175200.v1.1 MD10G1164300.v1.1
prunus_persica Prupe.8G202900_v2.0.a1 Prupe.8G202900_v2.0.a1
pyrus_communis pycom05g16210 pycom10g14170
rosa_chinensis RchiOBHm_Chr6g0284851
rosa_laevigata RLG00000012707
rosa_multiflora Rmu_sc0000686.1_g000017
rosa_roxburghii Rroxscaffold_7G00184300
rosa_rugosa Rorug06G0167500
rosa_samantha Rh6AG280200 Rh6BG280500 Rh6CG282500
rosa_wichuraiana Rw6G024080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 258, 382
AcsI RAATTY 2 cut(s) 104, 221
AcyI GRCGYC 1 cut(s) 378
AfaI GTAC 1 cut(s) 272
AfiI CCNNNNNNNGG 1 cut(s) 264
AjnI CCWGG 2 cut(s) 121, 300
AjuI GAANNNNNNNTTGG 2 cut(s) 213, 245
Alw26I GTCTC 3 cut(s) 3, 43, 228
ApeKI GCWGC 2 cut(s) 146, 286
ApoI RAATTY 2 cut(s) 104, 221
AseI ATTAAT 1 cut(s) 240
AspS9I GGNCC 1 cut(s) 115
AsuHPI GGTGA 1 cut(s) 140
AsuII TTCGAA 1 cut(s) 177
AvaII GGWCC 1 cut(s) 115
BbvCI CCTCAGC 1 cut(s) 33
BbvI GCAGC 2 cut(s) 158, 298
BccI CCATC 4 cut(s) 74, 126, 208, 260
BciT130I CCWGG 2 cut(s) 123, 302
BcoDI GTCTC 3 cut(s) 3, 43, 228
BfaI CTAG 1 cut(s) 204
BisI GCNGC 2 cut(s) 147, 287
BlpI GCTNAGC 1 cut(s) 367
BlsI GCNGC 2 cut(s) 148, 288
Bme1390I CCNGG 2 cut(s) 123, 302
Bme18I GGWCC 1 cut(s) 115
BmgT120I GGNCC 1 cut(s) 115
BmrFI CCNGG 2 cut(s) 123, 302
BmsI GCATC 1 cut(s) 257
BpmI CTGGAG 2 cut(s) 27, 320
Bpu10I CCTNAGC 1 cut(s) 33
Bpu1102I GCTNAGC 1 cut(s) 367
Bpu14I TTCGAA 1 cut(s) 177
Bsa29I ATCGAT 1 cut(s) 78
BsaHI GRCGYC 1 cut(s) 378
BsaI GGTCTC 1 cut(s) 43
Bsc4I CCNNNNNNNGG 1 cut(s) 264
Bse1I ACTGG 1 cut(s) 337
Bse3DI GCAATG 1 cut(s) 216
BseBI CCWGG 2 cut(s) 123, 302
BseCI ATCGAT 1 cut(s) 78
BseGI GGATG 4 cut(s) 118, 248, 252, 273
BseLI CCNNNNNNNGG 1 cut(s) 264
BseMI GCAATG 1 cut(s) 216
BseMII CTCAG 1 cut(s) 24
BseNI ACTGG 1 cut(s) 337
BseRI GAGGAG 2 cut(s) 267, 323
BseXI GCAGC 2 cut(s) 158, 298
BshVI ATCGAT 1 cut(s) 78
BslI CCNNNNNNNGG 1 cut(s) 264
BsmAI GTCTC 3 cut(s) 3, 43, 228
Bso31I GGTCTC 1 cut(s) 43
Bsp119I TTCGAA 1 cut(s) 177
Bsp1407I TGTACA 1 cut(s) 270
Bsp143I GATC 1 cut(s) 52
Bsp1720I GCTNAGC 1 cut(s) 367
BspACI CCGC 2 cut(s) 258, 382
BspCNI CTCAG 1 cut(s) 25
BspDI ATCGAT 1 cut(s) 78
BspT104I TTCGAA 1 cut(s) 177
BspTNI GGTCTC 1 cut(s) 43
BsrDI GCAATG 1 cut(s) 216
BsrGI TGTACA 1 cut(s) 270
BsrI ACTGG 1 cut(s) 337
BssMI GATC 1 cut(s) 52
BssNI GRCGYC 1 cut(s) 378
Bst2UI CCWGG 2 cut(s) 123, 302
Bst4CI ACNGT 2 cut(s) 50, 194
BstACI GRCGYC 1 cut(s) 378
BstAPI GCANNNNNTGC 1 cut(s) 63
BstAUI TGTACA 1 cut(s) 270
BstBI TTCGAA 1 cut(s) 177
BstC8I GCNNGC 1 cut(s) 68
BstDEI CTNAG 2 cut(s) 33, 367
BstF5I GGATG 4 cut(s) 118, 248, 252, 273
BstKTI GATC 1 cut(s) 55
BstMAI GTCTC 3 cut(s) 3, 43, 228
BstMBI GATC 1 cut(s) 52
BstMWI GCNNNNNNNGC 1 cut(s) 63
BstNI CCWGG 2 cut(s) 123, 302
BstSCI CCNGG 2 cut(s) 121, 300
BstV1I GCAGC 2 cut(s) 158, 298
BstXI CCANNNNNNTGG 1 cut(s) 125
Bsu15I ATCGAT 1 cut(s) 78
BsuTUI ATCGAT 1 cut(s) 78
BtsCI GGATG 4 cut(s) 118, 248, 252, 273
BtsI GCAGTG 1 cut(s) 198
BtsIMutI CAGTG 2 cut(s) 55, 198
Cac8I GCNNGC 1 cut(s) 68
Cfr13I GGNCC 1 cut(s) 115
ClaI ATCGAT 1 cut(s) 78
CseI GACGC 2 cut(s) 170, 367
Csp6I GTAC 1 cut(s) 271
CviJI RGCY 7 cut(s) 5, 32, 70, 149, 289, 366, 371
CviKI_1 RGCY 7 cut(s) 5, 32, 70, 149, 289, 366, 371
CviQI GTAC 1 cut(s) 271
DdeI CTNAG 2 cut(s) 33, 367
DpnI GATC 1 cut(s) 54
DpnII GATC 1 cut(s) 52
Eco31I GGTCTC 1 cut(s) 43
Eco47I GGWCC 1 cut(s) 115
EcoRII CCWGG 2 cut(s) 121, 300
FaiI YATR 3 cut(s) 24, 93, 409
FauI CCCGC 1 cut(s) 265
Fnu4HI GCNGC 2 cut(s) 147, 287
FokI GGATG 4 cut(s) 105, 235, 239, 280
Fsp4HI GCNGC 2 cut(s) 147, 287
FspBI CTAG 1 cut(s) 204
GluI GCNGC 2 cut(s) 147, 287
GsuI CTGGAG 2 cut(s) 27, 320
HgaI GACGC 2 cut(s) 170, 367
Hin1I GRCGYC 1 cut(s) 378
HphI GGTGA 1 cut(s) 140
Hpy166II GTNNAC 1 cut(s) 273
Hpy8I GTNNAC 1 cut(s) 273
HpyCH4III ACNGT 2 cut(s) 50, 194
HpyCH4IV ACGT 1 cut(s) 228
HpyF10VI GCNNNNNNNGC 1 cut(s) 63
HpyF3I CTNAG 2 cut(s) 33, 367
HpySE526I ACGT 1 cut(s) 228
Hsp92I GRCGYC 1 cut(s) 378
Kzo9I GATC 1 cut(s) 52
LpnPI CCDG 7 cut(s) 52, 108, 135, 138, 287, 314, 350
Lsp1109I GCAGC 2 cut(s) 158, 298
LweI GCATC 1 cut(s) 257
MaeI CTAG 1 cut(s) 204
MaeII ACGT 1 cut(s) 228
MaeIII GTNAC 2 cut(s) 97, 188
MalI GATC 1 cut(s) 54
MboI GATC 1 cut(s) 52
MboII GAAGA 1 cut(s) 191
MluCI AATT 4 cut(s) 104, 221, 241, 357
MmeI TCCRAC 1 cut(s) 211
MnlI CCTC 8 cut(s) 28, 106, 127, 258, 288, 300, 344, 360
MseI TTAA 1 cut(s) 240
MslI CAYNNNNRTG 1 cut(s) 123
MspR9I CCNGG 2 cut(s) 123, 302
MvaI CCWGG 2 cut(s) 123, 302
MwoI GCNNNNNNNGC 1 cut(s) 63
NdeII GATC 1 cut(s) 52
NmuCI GTSAC 1 cut(s) 188
NspV TTCGAA 1 cut(s) 177
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PkrI GCNGC 2 cut(s) 148, 288
PshBI ATTAAT 1 cut(s) 240
Psp6I CCWGG 2 cut(s) 121, 300
PspGI CCWGG 2 cut(s) 121, 300
PspPI GGNCC 1 cut(s) 115
RsaI GTAC 1 cut(s) 272
RsaNI GTAC 1 cut(s) 271
RseI CAYNNNNRTG 1 cut(s) 123
SaqAI TTAA 1 cut(s) 240
SatI GCNGC 2 cut(s) 147, 287
Sau3AI GATC 1 cut(s) 52
Sau96I GGNCC 1 cut(s) 115
ScrFI CCNGG 2 cut(s) 123, 302
SetI ASST 5 cut(s) 21, 39, 90, 138, 231
SfaNI GCATC 1 cut(s) 257
SfuI TTCGAA 1 cut(s) 177
SinI GGWCC 1 cut(s) 115
SmiMI CAYNNNNRTG 1 cut(s) 123
Sse9I AATT 4 cut(s) 104, 221, 241, 357
SsiI CCGC 2 cut(s) 258, 382
SspMI CTAG 1 cut(s) 204
StyD4I CCNGG 2 cut(s) 121, 300
TaaI ACNGT 2 cut(s) 50, 194
TaiI ACGT 1 cut(s) 231
TaqI TCGA 2 cut(s) 78, 177
TasI AATT 4 cut(s) 104, 221, 241, 357
TatI WGTACW 1 cut(s) 270
Tru1I TTAA 1 cut(s) 240
Tru9I TTAA 1 cut(s) 240
TscAI CASTG 2 cut(s) 55, 205
TseFI GTSAC 1 cut(s) 188
TseI GCWGC 2 cut(s) 146, 286
Tsp45I GTSAC 1 cut(s) 188
TspDTI ATGAA 1 cut(s) 331
TspRI CASTG 2 cut(s) 55, 205
VpaK11BI GGWCC 1 cut(s) 115
VspI ATTAAT 1 cut(s) 240
XapI RAATTY 2 cut(s) 104, 221
XspI CTAG 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.