Rorug06G0167500

Cytochrome b5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0013843
Physical Location & Seq
Reverse (-)
24508119 .. 24511484
3366 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0167500.1

Sequence Viewer

Length: 1518 bp
ATGGGCTTACTGGGTCTCGTTGGATTTGCGCTCCAATGCATTCATGTTCTTGCATGGCCTCTCTTTGCTCTGGTCTATCCTCTGTGTTCTTCCATTAGAGCAATCGAGACAGATTCCATTACAGATACTCACAAGATAAATACGTATTGGGTCGTCTTCTCATTGATCTTGCTATTTGAACATGCTTTTATGAAGCTCCTTGAATGGTTCCTGCTCTGGCCATACATTAGGCTACTGATTGTCTTCTTTTTGGTGATGCCACATTTTGATGGTGCCTTATATGTCTATAAACACCTTATCTTTCCTTGCCTCTCCGTGGATCCCCAAATTGTTATCAACTTGTTCAACAAGCGGAAGGAGTCCCTTGCGAGAGATAACTTTCTCTCTGAGGTTGAGAGATATGTCAAGGAGAATGGACCTGAAGATTTGGAAAAATATATTGCTTGCAAGTCAACGAGGACAAATCTCAACGTAAAAGAGTTCAATGCAGTTTCATCCATGGATAATGAAGCGTCAAAATCCATAACGCCAAATGGTGGACAGAATGATATCAAAGCTGTGCAGGTAATGGTGAAAAAGAAGGAAACAACTGCTAGCCAAGTTAGTCAGGCAGAGTCTAATCTCACTGTGAATGAAGATAAATTTTCTTCTAGGAAGACGAATGAAAAAGCCATTGAGGCTGCAGCCGACAGAGAAATTCTGAATACTCCACCTCCAAGGAAAGTCCAGAAGGAGTGGACCACAGTCAATCCGCTACCAACTCAAACTGATGCAACTTTTAATTCCCCTCTCGGTGATAATAAACACAAGGCTACATACGAGGCACTGAAGATAAAGAATGAGGGAGAGCCGAAGTTCACTGGGCCTGAAAACCGTACTGTTGCAGCCATGGAAATATATGAAAAGCCGGATGAAGTTGGAACAGCTATAGAAGATCCAGAGCCTCCTAAAGAAGTCCAGAAAGAGTGGACTTGTGCTCTCTGTCAGGTAACTACTGCATGTGAAAGAACTATGAATTCCCACCTGCAAGGGAGAAAACACAAGGCAGCATATGAGGCACTGAAAGCAGGGAACCAGGCATTTGTGCCCAAGATTGCCCCAGCTTCAACTGCAAAAACATCCAATCAACCAAATAAGGAGCCAGGAAAGAGTATTCGGAGCAGTGGATCAAAACAAAAAGTTACTGTAAATGAAAATGTGCAAGGCCAGAAAAATAGTAGTCCTGCTCCAATCACAAAGCACTATAAACCGAAAGAGCAGCTAGCAGATGGTGTATCCAGCAATGGCCAGAAAGGGAAAATTGCTATACACGTCGAGAAGGTACAAAGTCAACAACCAAAGCCAAATGAAGTTCCAAGGGTGAAGAGTTCTCCTATGCGGTGCGAAATTTGTGGCGTATGCTGTTCTGATGAGATTGACTTGATGTCTCATCTCAACGGAAAAAAACACAAGGAAAATGTGCAAGAGTACCTAATCAGGGTTGCAAGGATGAAAAAGCCTAGGGTCCTAGCTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyt-b5

Protein Analysis

505

Amino Acids

56.63

Weight (kDa)

8.94

Isoelectric Point (pI)

32.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TB2_DP1_HVA22 PF03134 27 - 102 1.8e-17 TB2/DP1, HVA22 family
zf-met PF12874 323 - 347 5e-08 Zinc-finger of C2H2 type
zf-met PF12874 460 - 483 3.1e-06 Zinc-finger of C2H2 type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013843)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48810
fragaria_vesca FvH4_2g19040
malus_domestica MD05G1175200.v1.1 MD10G1164300.v1.1
prunus_persica Prupe.8G202900_v2.0.a1 Prupe.8G202900_v2.0.a1
pyrus_communis pycom05g16210 pycom10g14170
rosa_chinensis RchiOBHm_Chr6g0284851
rosa_laevigata RLG00000012707
rosa_multiflora Rmu_sc0000686.1_g000017
rosa_roxburghii Rroxscaffold_7G00184300
rosa_rugosa Rorug06G0167500
rosa_samantha Rh6AG280200 Rh6BG280500 Rh6CG282500
rosa_wichuraiana Rw6G024080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1032
Acc36I ACCTGC 2 cut(s) 553, 1032
AccB1I GGYRCC 1 cut(s) 272
AccB7I CCANNNNNTGG 1 cut(s) 536
AciI CCGC 3 cut(s) 352, 752, 1378
AclWI GGATC 4 cut(s) 314, 327, 929, 1174
AcoI YGGCCR 2 cut(s) 218, 1285
AcsI RAATTY 4 cut(s) 641, 696, 1015, 1386
AcuI CTGAAG 2 cut(s) 441, 848
AfaI GTAC 3 cut(s) 877, 1323, 1469
AfiI CCNNNNNNNGG 3 cut(s) 316, 536, 1477
AflIII ACRYGT 1 cut(s) 1309
AgsI TTSAA 5 cut(s) 179, 203, 346, 484, 1107
AhdI GACNNNNNGTC 1 cut(s) 1423
AjiI CACGTC 1 cut(s) 1312
AjnI CCWGG 2 cut(s) 1074, 1141
AluBI AGCT 6 cut(s) 196, 557, 926, 1103, 1261, 1511
AluI AGCT 6 cut(s) 196, 557, 926, 1103, 1261, 1511
Alw21I GWGCWC 1 cut(s) 979
Alw26I GTCTC 3 cut(s) 20, 101, 1431
AlwI GGATC 4 cut(s) 314, 327, 929, 1174
AoxI GGCC 5 cut(s) 56, 218, 863, 1204, 1285
ApeKI GCWGC 5 cut(s) 680, 683, 884, 1046, 1258
ApoI RAATTY 4 cut(s) 641, 696, 1015, 1386
AspA2I CCTAGG 1 cut(s) 1499
AspLEI GCGC 1 cut(s) 31
AspS9I GGNCC 4 cut(s) 416, 738, 863, 1504
AsuHPI GGTGA 4 cut(s) 265, 583, 806, 1372
AsuNHI GCTAGC 2 cut(s) 593, 1261
AvaII GGWCC 3 cut(s) 416, 738, 1504
AvrII CCTAGG 1 cut(s) 1499
BaeGI GKGCMC 1 cut(s) 1089
BalI TGGCCA 2 cut(s) 220, 1287
BamHI GGATCC 1 cut(s) 319
BanI GGYRCC 1 cut(s) 272
BbsI GAAGAC 3 cut(s) 148, 235, 662
Bbv12I GWGCWC 1 cut(s) 979
BbvI GCAGC 5 cut(s) 667, 695, 896, 1058, 1270
BccI CCATC 2 cut(s) 263, 1262
BciT130I CCWGG 2 cut(s) 1076, 1143
BciVI GTATCC 1 cut(s) 1285
BcoDI GTCTC 3 cut(s) 20, 101, 1431
BfaI CTAG 5 cut(s) 594, 651, 1262, 1500, 1508
BfmI CTRYAG 2 cut(s) 681, 927
BfuAI ACCTGC 2 cut(s) 553, 1032
BfuI GTATCC 1 cut(s) 1285
BglI GCCNNNNNGGC 1 cut(s) 677
BisI GCNGC 5 cut(s) 681, 684, 885, 1047, 1259
BlnI CCTAGG 1 cut(s) 1499
BlsI GCNGC 5 cut(s) 682, 685, 886, 1048, 1260
Bme1390I CCNGG 2 cut(s) 1076, 1143
Bme18I GGWCC 3 cut(s) 416, 738, 1504
BmeRI GACNNNNNGTC 1 cut(s) 1423
BmgBI CACGTC 1 cut(s) 1312
BmgT120I GGNCC 4 cut(s) 416, 738, 863, 1504
BmiI GGNNCC 6 cut(s) 209, 274, 321, 1073, 1140, 1505
BmrFI CCNGG 2 cut(s) 1076, 1143
BmrI ACTGGG 2 cut(s) 20, 870
BmsI GCATC 2 cut(s) 246, 760
BmtI GCTAGC 2 cut(s) 597, 1265
BmuI ACTGGG 2 cut(s) 20, 870
BoxI GACNNNNGTC 1 cut(s) 743
BpiI GAAGAC 3 cut(s) 148, 235, 662
BsaAI YACGTR 1 cut(s) 144
BsaI GGTCTC 1 cut(s) 20
BsaJI CCNNGG 6 cut(s) 315, 498, 716, 888, 1355, 1499
BsaXI ACNNNNNCTCC 2 cut(s) 697, 727
Bsc4I CCNNNNNNNGG 3 cut(s) 316, 536, 1477
Bse1I ACTGG 2 cut(s) 15, 865
Bse3DI GCAATG 1 cut(s) 1288
BseBI CCWGG 2 cut(s) 1076, 1143
BseDI CCNNGG 6 cut(s) 315, 498, 716, 888, 1355, 1499
BseGI GGATG 4 cut(s) 494, 916, 1118, 1494
BseLI CCNNNNNNNGG 3 cut(s) 316, 536, 1477
BseMI GCAATG 1 cut(s) 1288
BseMII CTCAG 1 cut(s) 378
BseNI ACTGG 2 cut(s) 15, 865
BseSI GKGCMC 1 cut(s) 1089
BseXI GCAGC 5 cut(s) 667, 695, 896, 1058, 1270
BseYI CCCAGC 1 cut(s) 1099
BsgI GTGCAG 1 cut(s) 581
BshFI GGCC 5 cut(s) 58, 220, 865, 1206, 1287
BshNI GGYRCC 1 cut(s) 272
BsiHKAI GWGCWC 1 cut(s) 979
BsiSI CCGG 1 cut(s) 908
BslFI GGGAC 1 cut(s) 346
BslI CCNNNNNNNGG 3 cut(s) 316, 536, 1477
BsmAI GTCTC 3 cut(s) 20, 101, 1431
BsmFI GGGAC 1 cut(s) 346
BsmI GAATGC 1 cut(s) 39
BsnI GGCC 5 cut(s) 58, 220, 865, 1206, 1287
Bso31I GGTCTC 1 cut(s) 20
Bsp1286I GDGCHC 2 cut(s) 979, 1089
Bsp143I GATC 4 cut(s) 165, 319, 934, 1166
Bsp19I CCATGG 2 cut(s) 498, 888
BspACI CCGC 3 cut(s) 352, 752, 1378
BspANI GGCC 5 cut(s) 58, 220, 865, 1206, 1287
BspCNI CTCAG 1 cut(s) 379
BspLI GGNNCC 6 cut(s) 209, 274, 321, 1073, 1140, 1505
BspMAI CTGCAG 1 cut(s) 685
BspMI ACCTGC 2 cut(s) 553, 1032
BspOI GCTAGC 2 cut(s) 597, 1265
BspPI GGATC 4 cut(s) 314, 327, 929, 1174
BspT107I GGYRCC 1 cut(s) 272
BspTNI GGTCTC 1 cut(s) 20
BsrDI GCAATG 1 cut(s) 1288
BsrI ACTGG 2 cut(s) 15, 865
BssECI CCNNGG 6 cut(s) 315, 498, 716, 888, 1355, 1499
BssMI GATC 4 cut(s) 165, 319, 934, 1166
BssT1I CCWWGG 5 cut(s) 498, 716, 888, 1355, 1499
Bst2UI CCWGG 2 cut(s) 1076, 1143
Bst4CI ACNGT 5 cut(s) 628, 745, 875, 880, 1186
Bst6I CTCTTC 1 cut(s) 1358
BstBAI YACGTR 1 cut(s) 144
BstC8I GCNNGC 3 cut(s) 445, 595, 1263
BstDEI CTNAG 1 cut(s) 387
BstDSI CCRYGG 3 cut(s) 315, 498, 888
BstF5I GGATG 4 cut(s) 494, 916, 1118, 1494
BstHHI GCGC 1 cut(s) 31
BstKTI GATC 4 cut(s) 168, 322, 937, 1169
BstMAI GTCTC 3 cut(s) 20, 101, 1431
BstMBI GATC 4 cut(s) 165, 319, 934, 1166
BstMWI GCNNNNNNNGC 4 cut(s) 677, 1055, 1064, 1109
BstNI CCWGG 2 cut(s) 1076, 1143
BstNSI RCATGY 2 cut(s) 185, 1002
BstPAI GACNNNNGTC 1 cut(s) 743
BstSCI CCNGG 2 cut(s) 1074, 1141
BstSFI CTRYAG 2 cut(s) 681, 927
BstSLI GKGCMC 1 cut(s) 1089
BstSNI TACGTA 1 cut(s) 144
BstV1I GCAGC 5 cut(s) 667, 695, 896, 1058, 1270
BstV2I GAAGAC 3 cut(s) 148, 235, 662
BstX2I RGATCY 2 cut(s) 319, 934
BstYI RGATCY 2 cut(s) 319, 934
BsuI GTATCC 1 cut(s) 1285
BsuRI GGCC 5 cut(s) 58, 220, 865, 1206, 1287
BtgI CCRYGG 3 cut(s) 315, 498, 888
BtrI CACGTC 1 cut(s) 1312
BtsCI GGATG 4 cut(s) 494, 916, 1118, 1494
BtsI GCAGTG 1 cut(s) 1168
BtsIMutI CAGTG 5 cut(s) 624, 824, 858, 1058, 1168
BveI ACCTGC 2 cut(s) 553, 1032
Cac8I GCNNGC 3 cut(s) 445, 595, 1263
CfoI GCGC 1 cut(s) 31
Cfr13I GGNCC 4 cut(s) 416, 738, 863, 1504
CseI GACGC 1 cut(s) 501
Csp6I GTAC 3 cut(s) 876, 1322, 1468
CviAII CATG 6 cut(s) 44, 54, 182, 499, 889, 999
CviQI GTAC 3 cut(s) 876, 1322, 1468
DdeI CTNAG 1 cut(s) 387
DpnI GATC 4 cut(s) 167, 321, 936, 1168
DpnII GATC 4 cut(s) 165, 319, 934, 1166
DriI GACNNNNNGTC 1 cut(s) 1423
EaeI YGGCCR 2 cut(s) 218, 1285
Eam1104I CTCTTC 1 cut(s) 1358
Eam1105I GACNNNNNGTC 1 cut(s) 1423
EarI CTCTTC 1 cut(s) 1358
Eco105I TACGTA 1 cut(s) 144
Eco130I CCWWGG 5 cut(s) 498, 716, 888, 1355, 1499
Eco31I GGTCTC 1 cut(s) 20
Eco32I GATATC 1 cut(s) 550
Eco47I GGWCC 3 cut(s) 416, 738, 1504
Eco57I CTGAAG 2 cut(s) 441, 848
EcoO109I RGGNCCY 1 cut(s) 1504
EcoRI GAATTC 1 cut(s) 1015
EcoRII CCWGG 2 cut(s) 1074, 1141
EcoRV GATATC 1 cut(s) 550
EcoT14I CCWWGG 5 cut(s) 498, 716, 888, 1355, 1499
EcoT22I ATGCAT 1 cut(s) 41
ErhI CCWWGG 5 cut(s) 498, 716, 888, 1355, 1499
FaeI CATG 6 cut(s) 47, 57, 185, 502, 892, 1002
FaqI GGGAC 1 cut(s) 346
FatI CATG 6 cut(s) 43, 53, 181, 498, 888, 998
FauNDI CATATG 1 cut(s) 1051
Fnu4HI GCNGC 5 cut(s) 681, 684, 885, 1047, 1259
FokI GGATG 4 cut(s) 481, 923, 1105, 1501
Fsp4HI GCNGC 5 cut(s) 681, 684, 885, 1047, 1259
FspBI CTAG 5 cut(s) 594, 651, 1262, 1500, 1508
GlaI GCGC 1 cut(s) 30
GluI GCNGC 5 cut(s) 681, 684, 885, 1047, 1259
GsaI CCCAGC 1 cut(s) 1103
HaeIII GGCC 5 cut(s) 58, 220, 865, 1206, 1287
HapII CCGG 1 cut(s) 908
HgaI GACGC 1 cut(s) 501
HhaI GCGC 1 cut(s) 31
Hin1II CATG 6 cut(s) 47, 57, 185, 502, 892, 1002
Hin6I GCGC 1 cut(s) 29
HinP1I GCGC 1 cut(s) 29
HincII GTYRAC 2 cut(s) 453, 1331
HindII GTYRAC 2 cut(s) 453, 1331
HinfI GANTC 3 cut(s) 113, 359, 614
HpaII CCGG 1 cut(s) 908
HphI GGTGA 4 cut(s) 265, 583, 806, 1372
Hpy166II GTNNAC 6 cut(s) 453, 539, 738, 858, 969, 1331
Hpy188I TCNGA 4 cut(s) 388, 702, 1158, 1408
Hpy188III TCNNGA 5 cut(s) 106, 727, 938, 958, 1315
Hpy8I GTNNAC 6 cut(s) 453, 539, 738, 858, 969, 1331
Hpy99I CGWCG 1 cut(s) 1316
HpyAV CCTTC 4 cut(s) 349, 574, 724, 1312
HpyCH4III ACNGT 5 cut(s) 628, 745, 875, 880, 1186
HpyCH4IV ACGT 3 cut(s) 143, 471, 1311
HpyF10VI GCNNNNNNNGC 4 cut(s) 677, 1055, 1064, 1109
HpyF3I CTNAG 1 cut(s) 387
HpySE526I ACGT 3 cut(s) 143, 471, 1311
Hsp92II CATG 6 cut(s) 47, 57, 185, 502, 892, 1002
HspAI GCGC 1 cut(s) 29
Kzo9I GATC 4 cut(s) 165, 319, 934, 1166
LmnI GCTCC 5 cut(s) 36, 201, 1138, 1158, 1231
Lsp1109I GCAGC 5 cut(s) 667, 695, 896, 1058, 1270
LweI GCATC 2 cut(s) 246, 760
MaeI CTAG 5 cut(s) 594, 651, 1262, 1500, 1508
MaeII ACGT 3 cut(s) 143, 471, 1311
MaeIII GTNAC 2 cut(s) 988, 1180
MalI GATC 4 cut(s) 167, 321, 936, 1168
MboI GATC 4 cut(s) 165, 319, 934, 1166
MflI RGATCY 2 cut(s) 319, 934
MhlI GDGCHC 2 cut(s) 979, 1089
MlsI TGGCCA 2 cut(s) 220, 1287
MluCI AATT 7 cut(s) 327, 641, 696, 781, 1015, 1299, 1386
MluNI TGGCCA 2 cut(s) 220, 1287
MlyI GAGTC 2 cut(s) 368, 623
MmeI TCCRAC 1 cut(s) 898
Mox20I TGGCCA 2 cut(s) 220, 1287
Mph1103I ATGCAT 1 cut(s) 41
MscI TGGCCA 2 cut(s) 220, 1287
MseI TTAA 1 cut(s) 780
MslI CAYNNNNRTG 1 cut(s) 267
Msp20I TGGCCA 2 cut(s) 220, 1287
MspI CCGG 1 cut(s) 908
MspR9I CCNGG 2 cut(s) 1076, 1143
Mva1269I GAATGC 1 cut(s) 39
MvaI CCWGG 2 cut(s) 1076, 1143
MwoI GCNNNNNNNGC 4 cut(s) 677, 1055, 1064, 1109
NcoI CCATGG 2 cut(s) 498, 888
NdeI CATATG 1 cut(s) 1051
NdeII GATC 4 cut(s) 165, 319, 934, 1166
NheI GCTAGC 2 cut(s) 593, 1261
NlaIII CATG 6 cut(s) 47, 57, 185, 502, 892, 1002
NlaIV GGNNCC 6 cut(s) 209, 274, 321, 1073, 1140, 1505
NsiI ATGCAT 1 cut(s) 41
NspI RCATGY 2 cut(s) 185, 1002
PaqCI CACCTGC 1 cut(s) 1032
PctI GAATGC 1 cut(s) 39
PfeI GAWTC 1 cut(s) 113
PflMI CCANNNNNTGG 1 cut(s) 536
PkrI GCNGC 5 cut(s) 682, 685, 886, 1048, 1260
PleI GAGTC 2 cut(s) 367, 622
PpsI GAGTC 2 cut(s) 367, 622
Ppu21I YACGTR 1 cut(s) 144
PpuMI RGGWCCY 1 cut(s) 1504
PshAI GACNNNNGTC 1 cut(s) 743
Psp5II RGGWCCY 1 cut(s) 1504
Psp6I CCWGG 2 cut(s) 1074, 1141
PspFI CCCAGC 1 cut(s) 1099
PspGI CCWGG 2 cut(s) 1074, 1141
PspN4I GGNNCC 6 cut(s) 209, 274, 321, 1073, 1140, 1505
PspPI GGNCC 4 cut(s) 416, 738, 863, 1504
PspPPI RGGWCCY 1 cut(s) 1504
PstI CTGCAG 1 cut(s) 685
PsuI RGATCY 2 cut(s) 319, 934
RsaI GTAC 3 cut(s) 877, 1323, 1469
RsaNI GTAC 3 cut(s) 876, 1322, 1468
RseI CAYNNNNRTG 1 cut(s) 267
SaqAI TTAA 1 cut(s) 780
SatI GCNGC 5 cut(s) 681, 684, 885, 1047, 1259
Sau3AI GATC 4 cut(s) 165, 319, 934, 1166
Sau96I GGNCC 4 cut(s) 416, 738, 863, 1504
SchI GAGTC 2 cut(s) 368, 623
ScrFI CCNGG 2 cut(s) 1076, 1143
SduI GDGCHC 2 cut(s) 979, 1089
SfaNI GCATC 2 cut(s) 246, 760
SfcI CTRYAG 2 cut(s) 681, 927
SinI GGWCC 3 cut(s) 416, 738, 1504
SmiMI CAYNNNNRTG 1 cut(s) 267
SnaBI TACGTA 1 cut(s) 144
Sse9I AATT 7 cut(s) 327, 641, 696, 781, 1015, 1299, 1386
SsiI CCGC 3 cut(s) 352, 752, 1378
SspMI CTAG 5 cut(s) 594, 651, 1262, 1500, 1508
StyD4I CCNGG 2 cut(s) 1074, 1141
StyI CCWWGG 5 cut(s) 498, 716, 888, 1355, 1499
TaaI ACNGT 5 cut(s) 628, 745, 875, 880, 1186
TaiI ACGT 3 cut(s) 146, 474, 1314
TaqI TCGA 2 cut(s) 105, 1314
TasI AATT 7 cut(s) 327, 641, 696, 781, 1015, 1299, 1386
TfiI GAWTC 1 cut(s) 113
Tru1I TTAA 1 cut(s) 780
Tru9I TTAA 1 cut(s) 780
TscAI CASTG 5 cut(s) 631, 831, 865, 1065, 1168
TseI GCWGC 5 cut(s) 680, 683, 884, 1046, 1258
TspGWI ACGGA 2 cut(s) 304, 1452
TspRI CASTG 5 cut(s) 631, 831, 865, 1065, 1168
Van91I CCANNNNNTGG 1 cut(s) 536
VpaK11BI GGWCC 3 cut(s) 416, 738, 1504
XapI RAATTY 4 cut(s) 641, 696, 1015, 1386
XceI RCATGY 2 cut(s) 185, 1002
XmaJI CCTAGG 1 cut(s) 1499
XspI CTAG 5 cut(s) 594, 651, 1262, 1500, 1508
Zsp2I ATGCAT 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.