Rmu_sc0000686.1_g000017

Cytochrome b5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000686.1
OG0013843
Physical Location & Seq
Forward (+)
83837 .. 85160
1324 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000686.1_g000017.1.cds

Sequence Viewer

Length: 414 bp
atgggcggggagaaaaaggtctacactctggacgaggtctacaaccacagcgaccgcaaagactgctggctcgtcattgatggcaaggtgtatgatgtgacaaaatttttggaggaccatcctggtggtgatgaggtcttattgtcatccacagggaaggatgcaactgatgattttgaggatgttggtcacagtactgcagctagagcaatgatggatgagtactatgtaggagacattgataaagcattcatctcagccaagtcgacaacgtacactcctcccaagcagcctcagtacaatccggacaaaacaaccgatttcatcaacaggctcctccagttccttgttcccctgctcattttgggtttggcttttgttgtgcgctcctataccaaatcaccaacagcctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyt-b5

Protein Analysis

137

Amino Acids

15.36

Weight (kDa)

4.82

Isoelectric Point (pI)

24.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013843)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48810
fragaria_vesca FvH4_2g19040
malus_domestica MD05G1175200.v1.1 MD10G1164300.v1.1
prunus_persica Prupe.8G202900_v2.0.a1 Prupe.8G202900_v2.0.a1
pyrus_communis pycom05g16210 pycom10g14170
rosa_chinensis RchiOBHm_Chr6g0284851
rosa_laevigata RLG00000012707
rosa_multiflora Rmu_sc0000686.1_g000017
rosa_roxburghii Rroxscaffold_7G00184300
rosa_rugosa Rorug06G0167500
rosa_samantha Rh6AG280200 Rh6BG280500 Rh6CG282500
rosa_wichuraiana Rw6G024080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 21, 39, 266
AccIII TCCGGA 1 cut(s) 304
AciI CCGC 2 cut(s) 6, 55
AcsI RAATTY 1 cut(s) 104
AfaI GTAC 4 cut(s) 196, 224, 275, 299
AjnI CCWGG 1 cut(s) 121
AluBI AGCT 1 cut(s) 203
AluI AGCT 1 cut(s) 203
Alw26I GTCTC 1 cut(s) 228
Aor13HI TCCGGA 1 cut(s) 304
ApeKI GCWGC 2 cut(s) 200, 289
ApoI RAATTY 1 cut(s) 104
ArsI GACNNNNNNTTYG 2 cut(s) 91, 123
AspLEI GCGC 1 cut(s) 387
AspS9I GGNCC 1 cut(s) 115
AsuHPI GGTGA 2 cut(s) 140, 393
AvaII GGWCC 1 cut(s) 115
BbvI GCAGC 2 cut(s) 212, 301
BccI CCATC 3 cut(s) 74, 126, 208
BciT130I CCWGG 1 cut(s) 123
BcoDI GTCTC 1 cut(s) 228
BfaI CTAG 1 cut(s) 204
BfmI CTRYAG 1 cut(s) 198
BisI GCNGC 2 cut(s) 201, 290
BlsI GCNGC 2 cut(s) 202, 291
BmcAI AGTACT 2 cut(s) 196, 224
Bme1390I CCNGG 1 cut(s) 123
Bme18I GGWCC 1 cut(s) 115
BmgT120I GGNCC 1 cut(s) 115
BmiI GGNNCC 1 cut(s) 335
BmrFI CCNGG 1 cut(s) 123
BmsI GCATC 1 cut(s) 151
BpmI CTGGAG 1 cut(s) 323
BsaWI WCCGGW 1 cut(s) 304
BsaXI ACNNNNNCTCC 2 cut(s) 262, 292
Bse1I ACTGG 1 cut(s) 340
Bse3DI GCAATG 1 cut(s) 216
BseAI TCCGGA 1 cut(s) 304
BseBI CCWGG 1 cut(s) 123
BseGI GGATG 5 cut(s) 118, 146, 166, 187, 223
BseMI GCAATG 1 cut(s) 216
BseMII CTCAG 2 cut(s) 270, 308
BseNI ACTGG 1 cut(s) 340
BseRI GAGGAG 2 cut(s) 270, 326
BseXI GCAGC 2 cut(s) 212, 301
Bsh1285I CGRYCG 1 cut(s) 55
BsiEI CGRYCG 1 cut(s) 55
BsiSI CCGG 1 cut(s) 305
BsmAI GTCTC 1 cut(s) 228
BsmI GAATGC 1 cut(s) 248
Bsp13I TCCGGA 1 cut(s) 304
BspACI CCGC 2 cut(s) 6, 55
BspCNI CTCAG 2 cut(s) 269, 307
BspEI TCCGGA 1 cut(s) 304
BspLI GGNNCC 1 cut(s) 335
BspMAI CTGCAG 1 cut(s) 202
BsrDI GCAATG 1 cut(s) 216
BsrI ACTGG 1 cut(s) 340
Bst2UI CCWGG 1 cut(s) 123
Bst4CI ACNGT 1 cut(s) 194
BstAPI GCANNNNNTGC 1 cut(s) 63
BstC8I GCNNGC 1 cut(s) 68
BstDEI CTNAG 2 cut(s) 256, 294
BstF5I GGATG 5 cut(s) 118, 146, 166, 187, 223
BstHHI GCGC 1 cut(s) 387
BstMAI GTCTC 1 cut(s) 228
BstMCI CGRYCG 1 cut(s) 55
BstMWI GCNNNNNNNGC 2 cut(s) 63, 206
BstNI CCWGG 1 cut(s) 123
BstSCI CCNGG 1 cut(s) 121
BstSFI CTRYAG 1 cut(s) 198
BstV1I GCAGC 2 cut(s) 212, 301
BstXI CCANNNNNNTGG 1 cut(s) 125
BtsCI GGATG 5 cut(s) 118, 146, 166, 187, 223
Cac8I GCNNGC 1 cut(s) 68
CfoI GCGC 1 cut(s) 387
Cfr13I GGNCC 1 cut(s) 115
Csp6I GTAC 4 cut(s) 195, 223, 274, 298
CviJI RGCY 7 cut(s) 70, 203, 260, 292, 334, 374, 410
CviKI_1 RGCY 7 cut(s) 70, 203, 260, 292, 334, 374, 410
CviQI GTAC 4 cut(s) 195, 223, 274, 298
DdeI CTNAG 2 cut(s) 256, 294
Eco47I GGWCC 1 cut(s) 115
EcoRII CCWGG 1 cut(s) 121
FaiI YATR 3 cut(s) 93, 228, 393
FblI GTMKAC 3 cut(s) 21, 39, 266
Fnu4HI GCNGC 2 cut(s) 201, 290
FokI GGATG 5 cut(s) 105, 133, 173, 194, 230
Fsp4HI GCNGC 2 cut(s) 201, 290
FspBI CTAG 1 cut(s) 204
GlaI GCGC 1 cut(s) 386
GluI GCNGC 2 cut(s) 201, 290
GsuI CTGGAG 1 cut(s) 323
HapII CCGG 1 cut(s) 305
HhaI GCGC 1 cut(s) 387
Hin6I GCGC 1 cut(s) 385
HinP1I GCGC 1 cut(s) 385
HincII GTYRAC 1 cut(s) 267
HindII GTYRAC 1 cut(s) 267
HpaII CCGG 1 cut(s) 305
HphI GGTGA 2 cut(s) 140, 393
Hpy166II GTNNAC 4 cut(s) 22, 40, 267, 276
Hpy188III TCNNGA 2 cut(s) 29, 305
Hpy8I GTNNAC 4 cut(s) 22, 40, 267, 276
HpyAV CCTTC 1 cut(s) 151
HpyCH4III ACNGT 1 cut(s) 194
HpyCH4IV ACGT 1 cut(s) 272
HpyCH4V TGCA 2 cut(s) 164, 200
HpyF10VI GCNNNNNNNGC 2 cut(s) 63, 206
HpyF3I CTNAG 2 cut(s) 256, 294
HpySE526I ACGT 1 cut(s) 272
HspAI GCGC 1 cut(s) 385
Kpn2I TCCGGA 1 cut(s) 304
LmnI GCTCC 2 cut(s) 339, 392
LpnPI CCDG 9 cut(s) 14, 52, 108, 135, 138, 316, 318, 353, 368
Lsp1109I GCAGC 2 cut(s) 212, 301
LweI GCATC 1 cut(s) 151
MaeI CTAG 1 cut(s) 204
MaeII ACGT 1 cut(s) 272
MaeIII GTNAC 2 cut(s) 97, 188
MluCI AATT 1 cut(s) 104
MnlI CCTC 7 cut(s) 28, 106, 127, 172, 291, 303, 347
MroI TCCGGA 1 cut(s) 304
MslI CAYNNNNRTG 1 cut(s) 123
MspI CCGG 1 cut(s) 305
MspR9I CCNGG 1 cut(s) 123
Mva1269I GAATGC 1 cut(s) 248
MvaI CCWGG 1 cut(s) 123
MwoI GCNNNNNNNGC 2 cut(s) 63, 206
NlaIV GGNNCC 1 cut(s) 335
NmuCI GTSAC 2 cut(s) 97, 188
PctI GAATGC 1 cut(s) 248
PflFI GACNNNGTC 1 cut(s) 35
PkrI GCNGC 2 cut(s) 202, 291
Psp6I CCWGG 1 cut(s) 121
PspGI CCWGG 1 cut(s) 121
PspN4I GGNNCC 1 cut(s) 335
PspPI GGNCC 1 cut(s) 115
PstI CTGCAG 1 cut(s) 202
PsyI GACNNNGTC 1 cut(s) 35
RsaI GTAC 4 cut(s) 196, 224, 275, 299
RsaNI GTAC 4 cut(s) 195, 223, 274, 298
RseI CAYNNNNRTG 1 cut(s) 123
SalI GTCGAC 1 cut(s) 265
SatI GCNGC 2 cut(s) 201, 290
Sau96I GGNCC 1 cut(s) 115
ScaI AGTACT 2 cut(s) 196, 224
ScrFI CCNGG 1 cut(s) 123
SetI ASST 6 cut(s) 21, 39, 90, 138, 205, 275
SfaNI GCATC 1 cut(s) 151
SfcI CTRYAG 1 cut(s) 198
SinI GGWCC 1 cut(s) 115
SmiMI CAYNNNNRTG 1 cut(s) 123
Sse9I AATT 1 cut(s) 104
SsiI CCGC 2 cut(s) 6, 55
SspMI CTAG 1 cut(s) 204
StyD4I CCNGG 1 cut(s) 121
TaaI ACNGT 1 cut(s) 194
TaiI ACGT 1 cut(s) 275
TaqI TCGA 1 cut(s) 266
TasI AATT 1 cut(s) 104
TatI WGTACW 3 cut(s) 194, 222, 297
TseFI GTSAC 2 cut(s) 97, 188
TseI GCWGC 2 cut(s) 200, 289
Tsp45I GTSAC 2 cut(s) 97, 188
TspDTI ATGAA 2 cut(s) 241, 313
Tth111I GACNNNGTC 1 cut(s) 35
VpaK11BI GGWCC 1 cut(s) 115
XapI RAATTY 1 cut(s) 104
XmiI GTMKAC 3 cut(s) 21, 39, 266
XspI CTAG 1 cut(s) 204
ZrmI AGTACT 2 cut(s) 196, 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.