AT5G50290

Wall-associated receptor kinase galacturonan-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013565
Physical Location & Seq
Forward (+)
20461777 .. 20464616
2840 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G50290.1

Sequence Viewer

Length: 912 bp
ATGAAAATCTTGATCTTGATTCTATCCTTTGTGACACTCTTTGAGATTTGCGTTGTGGACGCATGTCGATCATACTGTGGAAACATAACCGTTGATTATCCGTTTGGGATCCGAAACGGATGTGGGCATCCAGGGTATAGAGATCTATTGTTTTGTATGAACGATGTGTTGATGTTTCACATAAGTTCAGGTTCTTATAGAGTTTTGGACATCGATTACGCATATCAGTCCATAACACTGCATGATCCTCACATGTCGAACTGCGAAACCATCGTGCTCGGTGGCAAAGGCAATGGCTTTGAAGCTGAGGATTGGAGAACTCCATATTTCAATCCTACCTCAGATAATGTCTTTATGTTGATCGGATGTTCTCCTAAATCTCCTATATTTCAAGGCTTCCCGGAAAAGAAAGTGCCGTGCCGCAACATCTCTGGAATGAGCTGCGAAGAATACATGTCATGTCCAGCTTGGGACATGGTCGGATACAGACAACCGGGTATACATTCCGGGTCAGGTCCACCCATGTGTTGTGGGGTCGGGTTCGAATCCGTAAAAGCGATTAATCTAAGTAAGTTGGAGTGTGAAGGATACAGTAGTGCGTATAATCTAGCACCCTTGAAACTTAGAGGACCCTCTGATTGGGCTTATGGGATACGTGTTAAGTATGAACTCCAAGGAAGTGATGCGTTTTGTCGTGCGTGTGTTGCAACTTCTGGGACTTGTGGCTATGAACCTGCTGATGGTGGAGGGCTTAGACATGTTTGCATGTGTGACAACCATAATTCCACTACAAACTGTGATTCAGTTATATCACCAACCGGTGCATCATCAAGTGTTCGACCAAAAGCTATCGGATCACTGATCATCTACTTCATAGCTATGAACATAGGCTTTCAGAGAAGACAGCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind

Protein Analysis

303

Amino Acids

33.26

Weight (kDa)

6.37

Isoelectric Point (pI)

51.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 22 - 82 1.2e-14 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013565)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50290
fragaria_vesca FvH4_5g05500
malus_domestica MD06G1172100.v1.1 MD14G1178000.v1.1
prunus_persica Prupe.5G171500_v2.0.a1
pyrus_communis pycom06g15330 pycom14g14850
rosa_chinensis RchiOBHm_Chr7g0193341
rosa_laevigata RLG00000004278
rosa_multiflora Rmu_ssc0000092.1_g000009
rosa_roxburghii Rroxscaffold_3G00262200
rosa_rugosa Rorug07G0009000
rosa_samantha Rh7AG134800 Rh7AG135200 Rh7BG135500 Rh7CG138900 Rh7DG136900
rosa_wichuraiana Rw7G011520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 742
AccI GTMKAC 1 cut(s) 499
AciI CCGC 1 cut(s) 421
AclWI GGATC 4 cut(s) 103, 116, 239, 862
AfiI CCNNNNNNNGG 2 cut(s) 639, 740
AflIII ACRYGT 4 cut(s) 252, 453, 655, 757
AgeI ACCGGT 1 cut(s) 818
AgsI TTSAA 4 cut(s) 302, 331, 392, 619
AjnI CCWGG 1 cut(s) 130
AleI CACNNNNGTG 1 cut(s) 523
AluBI AGCT 5 cut(s) 305, 441, 467, 848, 878
AluI AGCT 5 cut(s) 305, 441, 467, 848, 878
Alw21I GWGCWC 1 cut(s) 279
AlwI GGATC 4 cut(s) 103, 116, 239, 862
ApeKI GCWGC 1 cut(s) 441
AseI ATTAAT 1 cut(s) 561
AsiGI ACCGGT 1 cut(s) 818
AspS9I GGNCC 2 cut(s) 515, 629
AsuC2I CCSGG 3 cut(s) 401, 495, 508
AsuHPI GGTGA 1 cut(s) 804
AsuII TTCGAA 1 cut(s) 543
AvaII GGWCC 2 cut(s) 515, 629
BaeI ACNNNNGTAYC 2 cut(s) 580, 613
BamHI GGATCC 1 cut(s) 108
BbsI GAAGAC 1 cut(s) 907
Bbv12I GWGCWC 1 cut(s) 279
BbvCI CCTCAGC 1 cut(s) 306
BbvI GCAGC 1 cut(s) 428
BccI CCATC 2 cut(s) 278, 734
BceAI ACGGC 1 cut(s) 400
BciT130I CCWGG 1 cut(s) 132
BciVI GTATCC 3 cut(s) 476, 581, 645
BclI TGATCA 1 cut(s) 861
BcnI CCSGG 3 cut(s) 401, 495, 508
BfaI CTAG 1 cut(s) 608
BfuAI ACCTGC 1 cut(s) 742
BfuI GTATCC 3 cut(s) 476, 581, 645
BglII AGATCT 1 cut(s) 142
BisI GCNGC 2 cut(s) 421, 442
BlsI GCNGC 2 cut(s) 422, 443
Bme1390I CCNGG 4 cut(s) 132, 401, 495, 508
Bme18I GGWCC 2 cut(s) 515, 629
BmgT120I GGNCC 2 cut(s) 515, 629
BmiI GGNNCC 2 cut(s) 110, 631
BmrFI CCNGG 4 cut(s) 132, 401, 495, 508
BmsI GCATC 3 cut(s) 136, 673, 833
BoxI GACNNNNGTC 1 cut(s) 63
BpiI GAAGAC 1 cut(s) 907
Bpu10I CCTNAGC 1 cut(s) 306
Bpu14I TTCGAA 1 cut(s) 543
BpuMI CCSGG 3 cut(s) 401, 495, 508
Bsa29I ATCGAT 1 cut(s) 213
BsaAI YACGTR 1 cut(s) 656
BsaJI CCNNGG 2 cut(s) 131, 673
BsaWI WCCGGW 1 cut(s) 818
Bsc4I CCNNNNNNNGG 2 cut(s) 639, 740
Bse118I RCCGGY 1 cut(s) 818
Bse3DI GCAATG 1 cut(s) 298
BseBI CCWGG 1 cut(s) 132
BseCI ATCGAT 1 cut(s) 213
BseDI CCNNGG 2 cut(s) 131, 673
BseGI GGATG 3 cut(s) 125, 127, 371
BseLI CCNNNNNNNGG 2 cut(s) 639, 740
BseMI GCAATG 1 cut(s) 298
BseMII CTCAG 2 cut(s) 297, 354
BseXI GCAGC 1 cut(s) 428
BshTI ACCGGT 1 cut(s) 818
BshVI ATCGAT 1 cut(s) 213
BsiHKAI GWGCWC 1 cut(s) 279
BsiSI CCGG 4 cut(s) 401, 494, 507, 819
BslFI GGGAC 2 cut(s) 485, 730
BslI CCNNNNNNNGG 2 cut(s) 639, 740
BsmFI GGGAC 2 cut(s) 485, 730
Bsp119I TTCGAA 1 cut(s) 543
Bsp1286I GDGCHC 1 cut(s) 279
Bsp143I GATC 8 cut(s) 12, 68, 108, 142, 244, 360, 854, 861
BspACI CCGC 1 cut(s) 421
BspCNI CTCAG 2 cut(s) 298, 353
BspDI ATCGAT 1 cut(s) 213
BspLI GGNNCC 2 cut(s) 110, 631
BspMI ACCTGC 1 cut(s) 742
BspPI GGATC 4 cut(s) 103, 116, 239, 862
BspT104I TTCGAA 1 cut(s) 543
BsrDI GCAATG 1 cut(s) 298
BsrFI RCCGGY 1 cut(s) 818
BssAI RCCGGY 1 cut(s) 818
BssECI CCNNGG 2 cut(s) 131, 673
BssMI GATC 8 cut(s) 12, 68, 108, 142, 244, 360, 854, 861
BssNAI GTATAC 1 cut(s) 500
BssT1I CCWWGG 1 cut(s) 673
Bst1107I GTATAC 1 cut(s) 500
Bst2UI CCWGG 1 cut(s) 132
Bst4CI ACNGT 4 cut(s) 77, 91, 593, 797
BstBAI YACGTR 1 cut(s) 656
BstBI TTCGAA 1 cut(s) 543
BstDEI CTNAG 5 cut(s) 306, 340, 566, 623, 752
BstF5I GGATG 3 cut(s) 125, 127, 371
BstKTI GATC 8 cut(s) 15, 71, 111, 145, 247, 363, 857, 864
BstMBI GATC 8 cut(s) 12, 68, 108, 142, 244, 360, 854, 861
BstMWI GCNNNNNNNGC 1 cut(s) 704
BstNI CCWGG 1 cut(s) 132
BstNSI RCATGY 5 cut(s) 66, 256, 457, 761, 769
BstPAI GACNNNNGTC 1 cut(s) 63
BstSCI CCNGG 4 cut(s) 130, 399, 493, 506
BstV1I GCAGC 1 cut(s) 428
BstV2I GAAGAC 1 cut(s) 907
BstX2I RGATCY 2 cut(s) 108, 142
BstYI RGATCY 2 cut(s) 108, 142
BstZ17I GTATAC 1 cut(s) 500
Bsu15I ATCGAT 1 cut(s) 213
BsuI GTATCC 3 cut(s) 476, 581, 645
BsuTUI ATCGAT 1 cut(s) 213
BtsCI GGATG 3 cut(s) 125, 127, 371
BtsI GCAGTG 1 cut(s) 236
BtsIMutI CAGTG 2 cut(s) 236, 857
BveI ACCTGC 1 cut(s) 742
Cfr10I RCCGGY 1 cut(s) 818
Cfr13I GGNCC 2 cut(s) 515, 629
ClaI ATCGAT 1 cut(s) 213
CseI GACGC 1 cut(s) 68
CspAI ACCGGT 1 cut(s) 818
CviAII CATG 9 cut(s) 63, 242, 253, 454, 459, 475, 523, 758, 766
DdeI CTNAG 5 cut(s) 306, 340, 566, 623, 752
DpnI GATC 8 cut(s) 14, 70, 110, 144, 246, 362, 856, 863
DpnII GATC 8 cut(s) 12, 68, 108, 142, 244, 360, 854, 861
Eco130I CCWWGG 1 cut(s) 673
Eco47I GGWCC 2 cut(s) 515, 629
EcoO109I RGGNCCY 1 cut(s) 629
EcoRII CCWGG 1 cut(s) 130
EcoT14I CCWWGG 1 cut(s) 673
ErhI CCWWGG 1 cut(s) 673
FaeI CATG 9 cut(s) 66, 245, 256, 457, 462, 478, 526, 761, 769
FaqI GGGAC 2 cut(s) 485, 730
FatI CATG 9 cut(s) 62, 241, 252, 453, 458, 474, 522, 757, 765
FbaI TGATCA 1 cut(s) 861
FblI GTMKAC 1 cut(s) 499
Fnu4HI GCNGC 2 cut(s) 421, 442
FokI GGATG 3 cut(s) 114, 132, 378
Fsp4HI GCNGC 2 cut(s) 421, 442
FspBI CTAG 1 cut(s) 608
GluI GCNGC 2 cut(s) 421, 442
HapII CCGG 4 cut(s) 401, 494, 507, 819
HgaI GACGC 1 cut(s) 68
Hin1II CATG 9 cut(s) 66, 245, 256, 457, 462, 478, 526, 761, 769
HinfI GANTC 3 cut(s) 19, 545, 800
HpaII CCGG 4 cut(s) 401, 494, 507, 819
HphI GGTGA 1 cut(s) 804
Hpy166II GTNNAC 3 cut(s) 58, 500, 518
Hpy188I TCNGA 7 cut(s) 113, 343, 365, 482, 637, 854, 897
Hpy188III TCNNGA 3 cut(s) 10, 16, 432
Hpy8I GTNNAC 3 cut(s) 58, 500, 518
HpyAV CCTTC 1 cut(s) 578
HpyCH4III ACNGT 4 cut(s) 77, 91, 593, 797
HpyCH4IV ACGT 1 cut(s) 655
HpyCH4V TGCA 4 cut(s) 241, 707, 765, 824
HpyF10VI GCNNNNNNNGC 1 cut(s) 704
HpyF3I CTNAG 5 cut(s) 306, 340, 566, 623, 752
HpySE526I ACGT 1 cut(s) 655
Hsp92II CATG 9 cut(s) 66, 245, 256, 457, 462, 478, 526, 761, 769
Ksp22I TGATCA 1 cut(s) 861
Kzo9I GATC 8 cut(s) 12, 68, 108, 142, 244, 360, 854, 861
Lsp1109I GCAGC 1 cut(s) 428
LweI GCATC 3 cut(s) 136, 673, 833
MaeI CTAG 1 cut(s) 608
MaeII ACGT 1 cut(s) 655
MaeIII GTNAC 2 cut(s) 31, 770
MalI GATC 8 cut(s) 14, 70, 110, 144, 246, 362, 856, 863
MboI GATC 8 cut(s) 12, 68, 108, 142, 244, 360, 854, 861
MboII GAAGA 2 cut(s) 458, 912
MflI RGATCY 2 cut(s) 108, 142
MhlI GDGCHC 1 cut(s) 279
MluCI AATT 1 cut(s) 781
MmeI TCCRAC 2 cut(s) 460, 555
MnlI CCTC 6 cut(s) 258, 301, 349, 620, 643, 740
MseI TTAA 2 cut(s) 561, 660
MslI CAYNNNNRTG 2 cut(s) 523, 878
MspI CCGG 4 cut(s) 401, 494, 507, 819
MspR9I CCNGG 4 cut(s) 132, 401, 495, 508
MvaI CCWGG 1 cut(s) 132
MwoI GCNNNNNNNGC 1 cut(s) 704
NciI CCSGG 3 cut(s) 401, 495, 508
NdeII GATC 8 cut(s) 12, 68, 108, 142, 244, 360, 854, 861
NlaIII CATG 9 cut(s) 66, 245, 256, 457, 462, 478, 526, 761, 769
NlaIV GGNNCC 2 cut(s) 110, 631
NmuCI GTSAC 2 cut(s) 31, 770
NspI RCATGY 5 cut(s) 66, 256, 457, 761, 769
NspV TTCGAA 1 cut(s) 543
OliI CACNNNNGTG 1 cut(s) 523
PciI ACATGT 3 cut(s) 252, 453, 757
PfeI GAWTC 3 cut(s) 19, 545, 800
PflFI GACNNNGTC 1 cut(s) 476
PfoI TCCNGGA 1 cut(s) 399
PinAI ACCGGT 1 cut(s) 818
PkrI GCNGC 2 cut(s) 422, 443
Ppu21I YACGTR 1 cut(s) 656
PpuMI RGGWCCY 1 cut(s) 629
PscI ACATGT 3 cut(s) 252, 453, 757
PshAI GACNNNNGTC 1 cut(s) 63
PshBI ATTAAT 1 cut(s) 561
Psp5II RGGWCCY 1 cut(s) 629
Psp6I CCWGG 1 cut(s) 130
PspGI CCWGG 1 cut(s) 130
PspN4I GGNNCC 2 cut(s) 110, 631
PspPI GGNCC 2 cut(s) 515, 629
PspPPI RGGWCCY 1 cut(s) 629
PsuI RGATCY 2 cut(s) 108, 142
PsyI GACNNNGTC 1 cut(s) 476
RseI CAYNNNNRTG 2 cut(s) 523, 878
SaqAI TTAA 2 cut(s) 561, 660
SatI GCNGC 2 cut(s) 421, 442
Sau3AI GATC 8 cut(s) 12, 68, 108, 142, 244, 360, 854, 861
Sau96I GGNCC 2 cut(s) 515, 629
ScrFI CCNGG 4 cut(s) 132, 401, 495, 508
SduI GDGCHC 1 cut(s) 279
SfaNI GCATC 3 cut(s) 136, 673, 833
SfuI TTCGAA 1 cut(s) 543
SinI GGWCC 2 cut(s) 515, 629
SmiMI CAYNNNNRTG 2 cut(s) 523, 878
Sse9I AATT 1 cut(s) 781
SsiI CCGC 1 cut(s) 421
SspMI CTAG 1 cut(s) 608
StyD4I CCNGG 4 cut(s) 130, 399, 493, 506
StyI CCWWGG 1 cut(s) 673
TaaI ACNGT 4 cut(s) 77, 91, 593, 797
TaiI ACGT 1 cut(s) 658
TaqI TCGA 5 cut(s) 67, 213, 257, 543, 838
TasI AATT 1 cut(s) 781
TauI GCSGC 1 cut(s) 423
TfiI GAWTC 3 cut(s) 19, 545, 800
Tru1I TTAA 2 cut(s) 561, 660
Tru9I TTAA 2 cut(s) 561, 660
TscAI CASTG 2 cut(s) 243, 864
TseFI GTSAC 2 cut(s) 31, 770
TseI GCWGC 1 cut(s) 441
Tsp45I GTSAC 2 cut(s) 31, 770
TspDTI ATGAA 6 cut(s) 17, 173, 681, 744, 862, 896
TspGWI ACGGA 3 cut(s) 90, 132, 538
TspRI CASTG 2 cut(s) 243, 864
Tth111I GACNNNGTC 1 cut(s) 476
VpaK11BI GGWCC 2 cut(s) 515, 629
VspI ATTAAT 1 cut(s) 561
XceI RCATGY 5 cut(s) 66, 256, 457, 761, 769
XmiI GTMKAC 1 cut(s) 499
XspI CTAG 1 cut(s) 608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.