MD06G1172100.v1.1

Wall-associated receptor kinase galacturonan-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0013565
Physical Location & Seq
Reverse (-)
31244971 .. 31245804
834 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1172100.v1.1.491

Sequence Viewer

Length: 834 bp
ATGAGTAGCTATAGTACTACTACTATGCTCATAATTCCCAGCTTCCTCCTCTGTGTTTTATTTTCTTCGTTTTTTCCATCAGGTTCGGCCAACACATGCAGATCGTATTGCGGAAACCTCACCGTAGATTACCCGTTCGCCGTCCGCTCCGGCTGCGGGCACCCGGGTTTTCGAGATCTCTTATACTGCATCAACGATGTGCTCATGTTTCACGTAAGCTCGGGATCTTACAGGGTCTTGGACATAGACTACGCTTACCAAGCCCTCACGTTGCATGAACCTCACATGTCAACATGTGACAGCATAGTACTAGGTGCCAAGGGCAATGGCTTCTCCGTCGAGCAATGGCGGGCTCCGTACATGAACCCGACGGCCGACAACGTGTTCATGCTGATCGGCTGCTCGGCTCAGTCGCCGCTCTTTCAAGGCTTTCCGGGGAAGCACTTGCCATGCAGAAACGTTTCGGGAATGAGTTGCGAGGAATACTATGATTGTCCAGCGTGGGATATGTTGGGCCATAGGAGGGTGGGTTCCATGTTTGGTTCGGGTCCACCTGAGTGTTGTGCGGTGCCGTTTGAGGCCATCAAGGCCGTTAATCTCACCAAGCTTCAGTGTGAAGGGTATAGCAGTGCTTACAATCTGGCCCCGCTGAAGCTGGATGGGGCCCATGGGTGGTCGTATGGTATCCGAGTGAAGTACTCTGCTCAAGAAAACGATGAGTTTTGTAGAGCGTGCGAGGCTACCAGTGGCACGTGCGGGTACGGTGCTGATGGCATTAGGCAATTGTGTATGTGTGGGAGCTTCAATTCCACGTCAAATTGTGATTCTGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind

Protein Analysis

278

Amino Acids

30.16

Weight (kDa)

5.59

Isoelectric Point (pI)

43.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 33 - 91 1.5e-12 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013565)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50290
fragaria_vesca FvH4_5g05500
malus_domestica MD06G1172100.v1.1 MD14G1178000.v1.1
prunus_persica Prupe.5G171500_v2.0.a1
pyrus_communis pycom06g15330 pycom14g14850
rosa_chinensis RchiOBHm_Chr7g0193341
rosa_laevigata RLG00000004278
rosa_multiflora Rmu_ssc0000092.1_g000009
rosa_roxburghii Rroxscaffold_3G00262200
rosa_rugosa Rorug07G0009000
rosa_samantha Rh7AG134800 Rh7AG135200 Rh7BG135500 Rh7CG138900 Rh7DG136900
rosa_wichuraiana Rw7G011520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 159, 314, 568
AccBSI CCGCTC 2 cut(s) 147, 418
AciI CCGC 8 cut(s) 111, 145, 156, 349, 416, 566, 647, 756
AclI AACGTT 1 cut(s) 459
AclWI GGATC 1 cut(s) 232
AcoI YGGCCR 2 cut(s) 87, 372
AcuI CTGAAG 2 cut(s) 593, 671
AcvI CACGTG 1 cut(s) 753
AfaI GTAC 5 cut(s) 16, 309, 359, 698, 761
AfiI CCNNNNNNNGG 3 cut(s) 156, 523, 672
AflIII ACRYGT 3 cut(s) 285, 293, 381
AgsI TTSAA 2 cut(s) 425, 805
AjiI CACGTC 1 cut(s) 813
AleI CACNNNNGTG 1 cut(s) 556
AloI GAACNNNNNNTCC 2 cut(s) 514, 546
AluBI AGCT 6 cut(s) 9, 42, 219, 607, 655, 801
AluI AGCT 6 cut(s) 9, 42, 219, 607, 655, 801
Alw21I GWGCWC 1 cut(s) 204
AlwI GGATC 1 cut(s) 232
Ama87I CYCGRG 2 cut(s) 163, 220
AoxI GGCC 7 cut(s) 87, 372, 514, 579, 588, 642, 663
ApaI GGGCCC 1 cut(s) 667
ApeKI GCWGC 2 cut(s) 153, 399
Asp700I GAANNNNTTC 1 cut(s) 460
AspS9I GGNCC 5 cut(s) 514, 548, 643, 663, 664
AsuC2I CCSGG 3 cut(s) 164, 165, 435
AsuHPI GGTGA 2 cut(s) 112, 592
AvaI CYCGRG 2 cut(s) 163, 220
AvaII GGWCC 1 cut(s) 548
BaeGI GKGCMC 2 cut(s) 162, 667
BanI GGYRCC 3 cut(s) 159, 314, 568
BanII GRGCYC 2 cut(s) 355, 667
BbrPI CACGTG 1 cut(s) 753
Bbv12I GWGCWC 1 cut(s) 204
BbvI GCAGC 2 cut(s) 140, 386
BccI CCATC 4 cut(s) 85, 590, 653, 764
BceAI ACGGC 4 cut(s) 125, 387, 556, 575
BciVI GTATCC 1 cut(s) 695
BcnI CCSGG 3 cut(s) 164, 165, 435
BfaI CTAG 1 cut(s) 311
BfmI CTRYAG 1 cut(s) 10
BfuI GTATCC 1 cut(s) 695
BglI GCCNNNNNGGC 1 cut(s) 587
BglII AGATCT 1 cut(s) 175
BisI GCNGC 3 cut(s) 154, 400, 416
BlsI GCNGC 3 cut(s) 155, 401, 417
BmcAI AGTACT 3 cut(s) 16, 309, 698
Bme1390I CCNGG 3 cut(s) 164, 165, 435
Bme18I GGWCC 1 cut(s) 548
BmeT110I CYCGRG 2 cut(s) 163, 220
BmgBI CACGTC 1 cut(s) 813
BmgT120I GGNCC 5 cut(s) 514, 548, 643, 663, 664
BmiI GGNNCC 9 cut(s) 161, 316, 354, 532, 549, 570, 645, 664, 665
BmrFI CCNGG 3 cut(s) 164, 165, 435
BmsI GCATC 1 cut(s) 198
BpuEI CTTGAG 1 cut(s) 690
BpuMI CCSGG 3 cut(s) 164, 165, 435
BsaAI YACGTR 2 cut(s) 214, 753
BsaJI CCNNGG 4 cut(s) 163, 318, 434, 667
BsaXI ACNNNNNCTCC 2 cut(s) 514, 544
Bsc4I CCNNNNNNNGG 3 cut(s) 156, 523, 672
Bse1I ACTGG 1 cut(s) 744
Bse3DI GCAATG 2 cut(s) 331, 350
BseDI CCNNGG 4 cut(s) 163, 318, 434, 667
BseGI GGATG 1 cut(s) 664
BseLI CCNNNNNNNGG 3 cut(s) 156, 523, 672
BseMI GCAATG 2 cut(s) 331, 350
BseMII CTCAG 2 cut(s) 422, 546
BseNI ACTGG 1 cut(s) 744
BseRI GAGGAG 1 cut(s) 38
BseSI GKGCMC 2 cut(s) 162, 667
BseX3I CGGCCG 1 cut(s) 372
BseXI GCAGC 2 cut(s) 140, 386
BseYI CCCAGC 1 cut(s) 38
Bsh1285I CGRYCG 1 cut(s) 375
BshFI GGCC 7 cut(s) 89, 374, 516, 581, 590, 644, 665
BshNI GGYRCC 3 cut(s) 159, 314, 568
BsiEI CGRYCG 1 cut(s) 375
BsiHKAI GWGCWC 1 cut(s) 204
BsiHKCI CYCGRG 2 cut(s) 163, 220
BsiSI CCGG 3 cut(s) 150, 164, 434
BslI CCNNNNNNNGG 3 cut(s) 156, 523, 672
BsnI GGCC 7 cut(s) 89, 374, 516, 581, 590, 644, 665
BsoBI CYCGRG 2 cut(s) 163, 220
Bsp120I GGGCCC 1 cut(s) 663
Bsp1286I GDGCHC 4 cut(s) 162, 204, 355, 667
Bsp143I GATC 4 cut(s) 101, 175, 224, 393
Bsp19I CCATGG 1 cut(s) 667
BspACI CCGC 8 cut(s) 111, 145, 156, 349, 416, 566, 647, 756
BspANI GGCC 7 cut(s) 89, 374, 516, 581, 590, 644, 665
BspCNI CTCAG 2 cut(s) 421, 547
BspLI GGNNCC 9 cut(s) 161, 316, 354, 532, 549, 570, 645, 664, 665
BspPI GGATC 1 cut(s) 232
BspT107I GGYRCC 3 cut(s) 159, 314, 568
BsrBI CCGCTC 2 cut(s) 147, 418
BsrDI GCAATG 2 cut(s) 331, 350
BsrI ACTGG 1 cut(s) 744
BssECI CCNNGG 4 cut(s) 163, 318, 434, 667
BssMI GATC 4 cut(s) 101, 175, 224, 393
BssT1I CCWWGG 2 cut(s) 318, 667
Bst4CI ACNGT 2 cut(s) 124, 764
BstBAI YACGTR 2 cut(s) 214, 753
BstC8I GCNNGC 3 cut(s) 158, 351, 733
BstDEI CTNAG 2 cut(s) 408, 555
BstDSI CCRYGG 1 cut(s) 667
BstF5I GGATG 1 cut(s) 664
BstKTI GATC 4 cut(s) 104, 178, 227, 396
BstMBI GATC 4 cut(s) 101, 175, 224, 393
BstMCI CGRYCG 1 cut(s) 375
BstMWI GCNNNNNNNGC 4 cut(s) 153, 260, 587, 737
BstNSI RCATGY 3 cut(s) 99, 289, 297
BstSCI CCNGG 3 cut(s) 162, 163, 433
BstSFI CTRYAG 1 cut(s) 10
BstSLI GKGCMC 2 cut(s) 162, 667
BstV1I GCAGC 2 cut(s) 140, 386
BstX2I RGATCY 2 cut(s) 175, 224
BstYI RGATCY 2 cut(s) 175, 224
BstZI CGGCCG 1 cut(s) 372
BsuI GTATCC 1 cut(s) 695
BsuRI GGCC 7 cut(s) 89, 374, 516, 581, 590, 644, 665
BtgI CCRYGG 1 cut(s) 667
BtrI CACGTC 1 cut(s) 813
BtsCI GGATG 1 cut(s) 664
BtsI GCAGTG 1 cut(s) 634
BtsIMutI CAGTG 3 cut(s) 617, 634, 751
Cac8I GCNNGC 3 cut(s) 158, 351, 733
Cfr13I GGNCC 5 cut(s) 514, 548, 643, 663, 664
Cfr9I CCCGGG 1 cut(s) 163
Csp6I GTAC 5 cut(s) 15, 308, 358, 697, 760
CviQI GTAC 5 cut(s) 15, 308, 358, 697, 760
DdeI CTNAG 2 cut(s) 408, 555
DpnI GATC 4 cut(s) 103, 177, 226, 395
DpnII GATC 4 cut(s) 101, 175, 224, 393
EaeI YGGCCR 2 cut(s) 87, 372
EagI CGGCCG 1 cut(s) 372
EclXI CGGCCG 1 cut(s) 372
Eco130I CCWWGG 2 cut(s) 318, 667
Eco24I GRGCYC 2 cut(s) 355, 667
Eco47I GGWCC 1 cut(s) 548
Eco52I CGGCCG 1 cut(s) 372
Eco57I CTGAAG 2 cut(s) 593, 671
Eco72I CACGTG 1 cut(s) 753
Eco88I CYCGRG 2 cut(s) 163, 220
EcoO109I RGGNCCY 1 cut(s) 663
EcoT14I CCWWGG 2 cut(s) 318, 667
EcoT38I GRGCYC 2 cut(s) 355, 667
ErhI CCWWGG 2 cut(s) 318, 667
FauI CCCGC 4 cut(s) 149, 342, 654, 749
Fnu4HI GCNGC 3 cut(s) 154, 400, 416
FokI GGATG 1 cut(s) 671
FriOI GRGCYC 2 cut(s) 355, 667
Fsp4HI GCNGC 3 cut(s) 154, 400, 416
FspBI CTAG 1 cut(s) 311
GluI GCNGC 3 cut(s) 154, 400, 416
GsaI CCCAGC 1 cut(s) 42
HaeIII GGCC 7 cut(s) 89, 374, 516, 581, 590, 644, 665
HapII CCGG 3 cut(s) 150, 164, 434
HincII GTYRAC 1 cut(s) 291
HindII GTYRAC 1 cut(s) 291
HindIII AAGCTT 1 cut(s) 605
HinfI GANTC 1 cut(s) 824
HpaII CCGG 3 cut(s) 150, 164, 434
HphI GGTGA 2 cut(s) 112, 592
Hpy166II GTNNAC 2 cut(s) 291, 551
Hpy188I TCNGA 1 cut(s) 689
Hpy188III TCNNGA 4 cut(s) 173, 222, 465, 707
Hpy8I GTNNAC 2 cut(s) 291, 551
Hpy99I CGWCG 2 cut(s) 341, 373
HpyAV CCTTC 1 cut(s) 611
HpyCH4III ACNGT 2 cut(s) 124, 764
HpyCH4IV ACGT 6 cut(s) 213, 269, 381, 459, 752, 812
HpyCH4V TGCA 4 cut(s) 99, 189, 274, 453
HpyF10VI GCNNNNNNNGC 4 cut(s) 153, 260, 587, 737
HpyF3I CTNAG 2 cut(s) 408, 555
HpySE526I ACGT 6 cut(s) 213, 269, 381, 459, 752, 812
Kzo9I GATC 4 cut(s) 101, 175, 224, 393
LmnI GCTCC 3 cut(s) 152, 358, 798
Lsp1109I GCAGC 2 cut(s) 140, 386
LweI GCATC 1 cut(s) 198
MaeI CTAG 1 cut(s) 311
MaeII ACGT 6 cut(s) 213, 269, 381, 459, 752, 812
MaeIII GTNAC 1 cut(s) 296
MalI GATC 4 cut(s) 103, 177, 226, 395
MbiI CCGCTC 2 cut(s) 147, 418
MboI GATC 4 cut(s) 101, 175, 224, 393
MboII GAAGA 1 cut(s) 57
MfeI CAATTG 1 cut(s) 782
MflI RGATCY 2 cut(s) 175, 224
MhlI GDGCHC 4 cut(s) 162, 204, 355, 667
MluCI AATT 4 cut(s) 33, 782, 805, 817
MnlI CCTC 9 cut(s) 56, 59, 128, 275, 291, 472, 516, 571, 730
MroXI GAANNNNTTC 1 cut(s) 460
MseI TTAA 1 cut(s) 594
MslI CAYNNNNRTG 1 cut(s) 556
MspA1I CMGCKG 1 cut(s) 649
MspI CCGG 3 cut(s) 150, 164, 434
MspR9I CCNGG 3 cut(s) 164, 165, 435
MunI CAATTG 1 cut(s) 782
MwoI GCNNNNNNNGC 4 cut(s) 153, 260, 587, 737
NciI CCSGG 3 cut(s) 164, 165, 435
NcoI CCATGG 1 cut(s) 667
NdeII GATC 4 cut(s) 101, 175, 224, 393
NlaIV GGNNCC 9 cut(s) 161, 316, 354, 532, 549, 570, 645, 664, 665
NmeAIII GCCGAG 1 cut(s) 383
NmuCI GTSAC 1 cut(s) 296
NspI RCATGY 3 cut(s) 99, 289, 297
OliI CACNNNNGTG 1 cut(s) 556
PciI ACATGT 2 cut(s) 285, 293
PdmI GAANNNNTTC 1 cut(s) 460
PfeI GAWTC 1 cut(s) 824
PkrI GCNGC 3 cut(s) 155, 401, 417
PmaCI CACGTG 1 cut(s) 753
PmlI CACGTG 1 cut(s) 753
Ppu21I YACGTR 2 cut(s) 214, 753
PscI ACATGT 2 cut(s) 285, 293
Psp1406I AACGTT 1 cut(s) 459
PspCI CACGTG 1 cut(s) 753
PspFI CCCAGC 1 cut(s) 38
PspN4I GGNNCC 9 cut(s) 161, 316, 354, 532, 549, 570, 645, 664, 665
PspOMI GGGCCC 1 cut(s) 663
PspPI GGNCC 5 cut(s) 514, 548, 643, 663, 664
PsuI RGATCY 2 cut(s) 175, 224
RsaI GTAC 5 cut(s) 16, 309, 359, 698, 761
RsaNI GTAC 5 cut(s) 15, 308, 358, 697, 760
RseI CAYNNNNRTG 1 cut(s) 556
SaqAI TTAA 1 cut(s) 594
SatI GCNGC 3 cut(s) 154, 400, 416
Sau3AI GATC 4 cut(s) 101, 175, 224, 393
Sau96I GGNCC 5 cut(s) 514, 548, 643, 663, 664
ScaI AGTACT 3 cut(s) 16, 309, 698
ScrFI CCNGG 3 cut(s) 164, 165, 435
SduI GDGCHC 4 cut(s) 162, 204, 355, 667
SfaNI GCATC 1 cut(s) 198
SfcI CTRYAG 1 cut(s) 10
SfiI GGCCNNNNNGGCC 1 cut(s) 587
SinI GGWCC 1 cut(s) 548
SmaI CCCGGG 1 cut(s) 165
SmiMI CAYNNNNRTG 1 cut(s) 556
SmlI CTYRAG 1 cut(s) 705
SmoI CTYRAG 1 cut(s) 705
Sse9I AATT 4 cut(s) 33, 782, 805, 817
SsiI CCGC 8 cut(s) 111, 145, 156, 349, 416, 566, 647, 756
SspMI CTAG 1 cut(s) 311
StyD4I CCNGG 3 cut(s) 162, 163, 433
StyI CCWWGG 2 cut(s) 318, 667
TaaI ACNGT 2 cut(s) 124, 764
TaiI ACGT 6 cut(s) 216, 272, 384, 462, 755, 815
TaqI TCGA 2 cut(s) 172, 339
TasI AATT 4 cut(s) 33, 782, 805, 817
TatI WGTACW 3 cut(s) 14, 307, 696
TauI GCSGC 1 cut(s) 418
TfiI GAWTC 1 cut(s) 824
Tru1I TTAA 1 cut(s) 594
Tru9I TTAA 1 cut(s) 594
TscAI CASTG 3 cut(s) 617, 634, 751
TseFI GTSAC 1 cut(s) 296
TseI GCWGC 2 cut(s) 153, 399
Tsp45I GTSAC 1 cut(s) 296
TspDTI ATGAA 3 cut(s) 291, 376, 377
TspGWI ACGGA 2 cut(s) 325, 345
TspMI CCCGGG 1 cut(s) 163
TspRI CASTG 3 cut(s) 617, 634, 751
VpaK11BI GGWCC 1 cut(s) 548
XceI RCATGY 3 cut(s) 99, 289, 297
XmaI CCCGGG 1 cut(s) 163
XmnI GAANNNNTTC 1 cut(s) 460
XspI CTAG 1 cut(s) 311
ZrmI AGTACT 3 cut(s) 16, 309, 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.