FvH4_5g05500

Wall-associated receptor kinase galacturonan-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0013565
Physical Location & Seq
Forward (+)
3228239 .. 3229604
1366 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g05500.t1

Sequence Viewer

Length: 900 bp
ATGAGTACCACTATGCTTATTCTTCTCAGCCTCCTGCATCTTCTAACACTAATTCCATCAACTTCAGGCAACACATGTAGATCCTTCTGTGGAAACATAACCATAGACTACCCCTTTGCCATCCATTCCGGGTGTGGCCACCCGGGTTTTCGAGATCTCTTATATTGTATCAACGATGTTCTTATGTTCCATATCAGCTCAGGCTCCTACAGGGTTCTGGAGATAGACTATGCATACCAATCTCTCACACTACATGACCCTCACATGTCAACTTGTGACACCATTGTGCTAGGTGCTAAGGGTAATGGCTTCGCAGTCGAGCAATGGCGGGCGCCATACATGAATCCATCCGCTGACAATGTGTTCATGCTGATAGGCTGCTCAGCTCAGTCGCCACTCTTCCAAGGCTTCCCAGGAAAGCACTTGCCATGTAGAAATGTTTCAGGCATGAGCTGTGAGGAGTACTATGGTTGCCCTGCTTGGGACCTTTTGGGCCATAGAATGGTTGGTTCCAAGTTCGGGACAGGCCCACCTGAGTGCTGTGCTGTGCCCTTTGAGGCTATCAAGTCAGTGAACCTCAGTAAGCTTCAGTGTGAAGGTTACAGCAGTGCTTATAGTCTAGCACCTTTGAGGCTTGATGGGGCTAATGGATGGTCTTATGGGATCCGTGTGAAATATTCTGTTCAAGAAAATGATGAGTTCTGTAGGTCGTGTGAGGCTACCGGAGGCACCTGTGGGTATGGTACTGATGGCATTAGGCAATTGTGCATGTGTGGGAGCTCAAATTCGACATCAAACTGCGATTCTGTGAAATCATCATCAAGAAGAACATACTCAAAGGCAGACACAGTCACTGCAGTGTCACTGATGTTCTTGCTAGCTTGGATTGCACAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind

Protein Analysis

300

Amino Acids

32.54

Weight (kDa)

6.43

Isoelectric Point (pI)

44.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 26 - 86 3e-14 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013565)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50290
fragaria_vesca FvH4_5g05500
malus_domestica MD06G1172100.v1.1 MD14G1178000.v1.1
prunus_persica Prupe.5G171500_v2.0.a1
pyrus_communis pycom06g15330 pycom14g14850
rosa_chinensis RchiOBHm_Chr7g0193341
rosa_laevigata RLG00000004278
rosa_multiflora Rmu_ssc0000092.1_g000009
rosa_roxburghii Rroxscaffold_3G00262200
rosa_rugosa Rorug07G0009000
rosa_samantha Rh7AG134800 Rh7AG135200 Rh7BG135500 Rh7CG138900 Rh7DG136900
rosa_wichuraiana Rw7G011520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 331, 728
AccB7I CCANNNNNTGG 1 cut(s) 502
AciI CCGC 2 cut(s) 328, 351
AclWI GGATC 3 cut(s) 75, 658, 671
AcoI YGGCCR 1 cut(s) 136
AcsI RAATTY 1 cut(s) 784
AcuI CTGAAG 2 cut(s) 48, 572
AcyI GRCGYC 1 cut(s) 332
AfaI GTAC 3 cut(s) 7, 464, 745
AfiI CCNNNNNNNGG 3 cut(s) 481, 502, 519
AflIII ACRYGT 2 cut(s) 74, 264
AgsI TTSAA 1 cut(s) 686
AjnI CCWGG 1 cut(s) 412
AleI CACNNNNGTG 4 cut(s) 284, 535, 857, 895
AluBI AGCT 6 cut(s) 198, 386, 453, 586, 780, 881
AluI AGCT 6 cut(s) 198, 386, 453, 586, 780, 881
Alw21I GWGCWC 1 cut(s) 782
AlwI GGATC 3 cut(s) 75, 658, 671
AlwNI CAGNNNCTG 1 cut(s) 854
Ama87I CYCGRG 1 cut(s) 142
AoxI GGCC 3 cut(s) 136, 493, 526
ApeKI GCWGC 1 cut(s) 378
ApoI RAATTY 1 cut(s) 784
ArsI GACNNNNNNTTYG 2 cut(s) 98, 130
Asp700I GAANNNNTTC 1 cut(s) 439
AspLEI GCGC 1 cut(s) 334
AspS9I GGNCC 3 cut(s) 484, 493, 527
AsuC2I CCSGG 3 cut(s) 130, 143, 144
AsuNHI GCTAGC 1 cut(s) 877
AvaI CYCGRG 1 cut(s) 142
AvaII GGWCC 1 cut(s) 484
BaeGI GKGCMC 1 cut(s) 552
BalI TGGCCA 1 cut(s) 138
BamHI GGATCC 1 cut(s) 663
BanI GGYRCC 2 cut(s) 331, 728
BanII GRGCYC 1 cut(s) 782
Bbv12I GWGCWC 1 cut(s) 782
BbvI GCAGC 1 cut(s) 365
BccI CCATC 6 cut(s) 64, 128, 355, 632, 645, 743
BciT130I CCWGG 1 cut(s) 414
BcnI CCSGG 3 cut(s) 130, 143, 144
BfaI CTAG 3 cut(s) 290, 620, 878
BfmI CTRYAG 3 cut(s) 208, 703, 855
BfoI RGCGCY 1 cut(s) 335
BglII AGATCT 1 cut(s) 154
BisI GCNGC 1 cut(s) 379
BlpI GCTNAGC 1 cut(s) 382
BlsI GCNGC 1 cut(s) 380
BmcAI AGTACT 1 cut(s) 464
Bme1390I CCNGG 4 cut(s) 130, 143, 144, 414
Bme18I GGWCC 1 cut(s) 484
BmeT110I CYCGRG 1 cut(s) 142
BmgT120I GGNCC 3 cut(s) 484, 493, 527
BmiI GGNNCC 6 cut(s) 205, 333, 485, 511, 665, 730
BmrFI CCNGG 4 cut(s) 130, 143, 144, 414
BmsI GCATC 1 cut(s) 46
BmtI GCTAGC 1 cut(s) 881
BpmI CTGGAG 1 cut(s) 239
Bpu10I CCTNAGC 2 cut(s) 199, 297
Bpu1102I GCTNAGC 1 cut(s) 382
BpuMI CCSGG 3 cut(s) 130, 143, 144
BsaHI GRCGYC 1 cut(s) 332
BsaJI CCNNGG 3 cut(s) 142, 403, 412
BsaWI WCCGGW 1 cut(s) 722
Bsc4I CCNNNNNNNGG 3 cut(s) 481, 502, 519
Bse3DI GCAATG 1 cut(s) 329
BseBI CCWGG 1 cut(s) 414
BseDI CCNNGG 3 cut(s) 142, 403, 412
BseGI GGATG 3 cut(s) 120, 347, 656
BseLI CCNNNNNNNGG 3 cut(s) 481, 502, 519
BseMI GCAATG 1 cut(s) 329
BseMII CTCAG 6 cut(s) 40, 213, 396, 401, 525, 592
BseRI GAGGAG 1 cut(s) 473
BseSI GKGCMC 1 cut(s) 552
BseXI GCAGC 1 cut(s) 365
BshFI GGCC 3 cut(s) 138, 495, 528
BshNI GGYRCC 2 cut(s) 331, 728
BsiHKAI GWGCWC 1 cut(s) 782
BsiHKCI CYCGRG 1 cut(s) 142
BsiSI CCGG 3 cut(s) 129, 143, 723
BslFI GGGAC 2 cut(s) 497, 535
BslI CCNNNNNNNGG 3 cut(s) 481, 502, 519
BsmFI GGGAC 2 cut(s) 497, 535
BsnI GGCC 3 cut(s) 138, 495, 528
BsoBI CYCGRG 1 cut(s) 142
Bsp1286I GDGCHC 2 cut(s) 552, 782
Bsp143I GATC 3 cut(s) 80, 154, 663
Bsp1720I GCTNAGC 1 cut(s) 382
BspACI CCGC 2 cut(s) 328, 351
BspANI GGCC 3 cut(s) 138, 495, 528
BspCNI CTCAG 6 cut(s) 39, 212, 395, 400, 526, 591
BspLI GGNNCC 6 cut(s) 205, 333, 485, 511, 665, 730
BspMAI CTGCAG 1 cut(s) 859
BspOI GCTAGC 1 cut(s) 881
BspPI GGATC 3 cut(s) 75, 658, 671
BspT107I GGYRCC 2 cut(s) 331, 728
BsrDI GCAATG 1 cut(s) 329
BssECI CCNNGG 3 cut(s) 142, 403, 412
BssMI GATC 3 cut(s) 80, 154, 663
BssNI GRCGYC 1 cut(s) 332
BssT1I CCWWGG 1 cut(s) 403
Bst2UI CCWGG 1 cut(s) 414
Bst4CI ACNGT 1 cut(s) 850
Bst6I CTCTTC 1 cut(s) 404
BstACI GRCGYC 1 cut(s) 332
BstC8I GCNNGC 2 cut(s) 330, 879
BstDEI CTNAG 7 cut(s) 26, 199, 297, 382, 387, 534, 578
BstF5I GGATG 3 cut(s) 120, 347, 656
BstH2I RGCGCY 1 cut(s) 335
BstHHI GCGC 1 cut(s) 334
BstKTI GATC 3 cut(s) 83, 157, 666
BstMBI GATC 3 cut(s) 80, 154, 663
BstMWI GCNNNNNNNGC 1 cut(s) 887
BstNI CCWGG 1 cut(s) 414
BstNSI RCATGY 3 cut(s) 78, 268, 772
BstSCI CCNGG 4 cut(s) 128, 141, 142, 412
BstSFI CTRYAG 3 cut(s) 208, 703, 855
BstSLI GKGCMC 1 cut(s) 552
BstV1I GCAGC 1 cut(s) 365
BstX2I RGATCY 3 cut(s) 80, 154, 663
BstYI RGATCY 3 cut(s) 80, 154, 663
BsuRI GGCC 3 cut(s) 138, 495, 528
BtsCI GGATG 3 cut(s) 120, 347, 656
BtsI GCAGTG 3 cut(s) 613, 852, 864
BtsIMutI CAGTG 6 cut(s) 576, 596, 613, 852, 863, 864
Cac8I GCNNGC 2 cut(s) 330, 879
CaiI CAGNNNCTG 1 cut(s) 854
CfoI GCGC 1 cut(s) 334
Cfr13I GGNCC 3 cut(s) 484, 493, 527
Cfr9I CCCGGG 1 cut(s) 142
Csp6I GTAC 3 cut(s) 6, 463, 744
CviAII CATG 8 cut(s) 75, 254, 265, 340, 367, 429, 448, 769
CviQI GTAC 3 cut(s) 6, 463, 744
DdeI CTNAG 7 cut(s) 26, 199, 297, 382, 387, 534, 578
DinI GGCGCC 1 cut(s) 333
DpnI GATC 3 cut(s) 82, 156, 665
DpnII GATC 3 cut(s) 80, 154, 663
EaeI YGGCCR 1 cut(s) 136
Eam1104I CTCTTC 1 cut(s) 404
EarI CTCTTC 1 cut(s) 404
Ecl136II GAGCTC 1 cut(s) 780
Eco130I CCWWGG 1 cut(s) 403
Eco24I GRGCYC 1 cut(s) 782
Eco47I GGWCC 1 cut(s) 484
Eco53kI GAGCTC 1 cut(s) 780
Eco57I CTGAAG 2 cut(s) 48, 572
Eco88I CYCGRG 1 cut(s) 142
EcoICRI GAGCTC 1 cut(s) 780
EcoO109I RGGNCCY 1 cut(s) 484
EcoRII CCWGG 1 cut(s) 412
EcoT14I CCWWGG 1 cut(s) 403
EcoT22I ATGCAT 1 cut(s) 235
EcoT38I GRGCYC 1 cut(s) 782
EgeI GGCGCC 1 cut(s) 333
EheI GGCGCC 1 cut(s) 333
ErhI CCWWGG 1 cut(s) 403
FaeI CATG 8 cut(s) 78, 257, 268, 343, 370, 432, 451, 772
FaqI GGGAC 2 cut(s) 497, 535
FatI CATG 8 cut(s) 74, 253, 264, 339, 366, 428, 447, 768
FauI CCCGC 1 cut(s) 321
Fnu4HI GCNGC 1 cut(s) 379
FokI GGATG 3 cut(s) 107, 334, 663
FriOI GRGCYC 1 cut(s) 782
Fsp4HI GCNGC 1 cut(s) 379
FspBI CTAG 3 cut(s) 290, 620, 878
GlaI GCGC 1 cut(s) 333
GluI GCNGC 1 cut(s) 379
GsuI CTGGAG 1 cut(s) 239
HaeII RGCGCY 1 cut(s) 335
HaeIII GGCC 3 cut(s) 138, 495, 528
HapII CCGG 3 cut(s) 129, 143, 723
HhaI GCGC 1 cut(s) 334
Hin1I GRCGYC 1 cut(s) 332
Hin1II CATG 8 cut(s) 78, 257, 268, 343, 370, 432, 451, 772
Hin6I GCGC 1 cut(s) 332
HinP1I GCGC 1 cut(s) 332
HincII GTYRAC 1 cut(s) 270
HindII GTYRAC 1 cut(s) 270
HindIII AAGCTT 1 cut(s) 584
HinfI GANTC 2 cut(s) 343, 803
HpaII CCGG 3 cut(s) 129, 143, 723
Hpy166II GTNNAC 2 cut(s) 270, 574
Hpy188III TCNNGA 5 cut(s) 152, 218, 520, 686, 822
Hpy8I GTNNAC 2 cut(s) 270, 574
HpyAV CCTTC 2 cut(s) 94, 590
HpyCH4III ACNGT 1 cut(s) 850
HpyCH4V TGCA 5 cut(s) 37, 233, 768, 857, 890
HpyF10VI GCNNNNNNNGC 1 cut(s) 887
HpyF3I CTNAG 7 cut(s) 26, 199, 297, 382, 387, 534, 578
Hsp92I GRCGYC 1 cut(s) 332
Hsp92II CATG 8 cut(s) 78, 257, 268, 343, 370, 432, 451, 772
HspAI GCGC 1 cut(s) 332
KasI GGCGCC 1 cut(s) 331
Kzo9I GATC 3 cut(s) 80, 154, 663
LmnI GCTCC 2 cut(s) 209, 777
Lsp1109I GCAGC 1 cut(s) 365
LweI GCATC 1 cut(s) 46
MaeI CTAG 3 cut(s) 290, 620, 878
MaeIII GTNAC 4 cut(s) 275, 599, 850, 861
MalI GATC 3 cut(s) 82, 156, 665
MboI GATC 3 cut(s) 80, 154, 663
MboII GAAGA 4 cut(s) 14, 32, 391, 837
MfeI CAATTG 1 cut(s) 761
MflI RGATCY 3 cut(s) 80, 154, 663
MhlI GDGCHC 2 cut(s) 552, 782
MlsI TGGCCA 1 cut(s) 138
MluCI AATT 3 cut(s) 51, 761, 784
MluNI TGGCCA 1 cut(s) 138
Mly113I GGCGCC 1 cut(s) 332
MnlI CCTC 8 cut(s) 41, 270, 451, 550, 587, 624, 709, 719
Mox20I TGGCCA 1 cut(s) 138
Mph1103I ATGCAT 1 cut(s) 235
MroXI GAANNNNTTC 1 cut(s) 439
MscI TGGCCA 1 cut(s) 138
MslI CAYNNNNRTG 4 cut(s) 284, 535, 857, 895
Msp20I TGGCCA 1 cut(s) 138
MspA1I CMGCKG 1 cut(s) 353
MspI CCGG 3 cut(s) 129, 143, 723
MspR9I CCNGG 4 cut(s) 130, 143, 144, 414
MunI CAATTG 1 cut(s) 761
MvaI CCWGG 1 cut(s) 414
MwoI GCNNNNNNNGC 1 cut(s) 887
NarI GGCGCC 1 cut(s) 332
NciI CCSGG 3 cut(s) 130, 143, 144
NdeII GATC 3 cut(s) 80, 154, 663
NheI GCTAGC 1 cut(s) 877
NlaIII CATG 8 cut(s) 78, 257, 268, 343, 370, 432, 451, 772
NlaIV GGNNCC 6 cut(s) 205, 333, 485, 511, 665, 730
NmuCI GTSAC 3 cut(s) 275, 850, 861
NsiI ATGCAT 1 cut(s) 235
NspI RCATGY 3 cut(s) 78, 268, 772
OliI CACNNNNGTG 4 cut(s) 284, 535, 857, 895
PciI ACATGT 2 cut(s) 74, 264
PdmI GAANNNNTTC 1 cut(s) 439
PfeI GAWTC 2 cut(s) 343, 803
PflFI GACNNNGTC 1 cut(s) 848
PflMI CCANNNNNTGG 1 cut(s) 502
PkrI GCNGC 1 cut(s) 380
PluTI GGCGCC 1 cut(s) 335
PpuMI RGGWCCY 1 cut(s) 484
PscI ACATGT 2 cut(s) 74, 264
Psp124BI GAGCTC 1 cut(s) 782
Psp5II RGGWCCY 1 cut(s) 484
Psp6I CCWGG 1 cut(s) 412
PspGI CCWGG 1 cut(s) 412
PspN4I GGNNCC 6 cut(s) 205, 333, 485, 511, 665, 730
PspPI GGNCC 3 cut(s) 484, 493, 527
PspPPI RGGWCCY 1 cut(s) 484
PstI CTGCAG 1 cut(s) 859
PstNI CAGNNNCTG 1 cut(s) 854
PsuI RGATCY 3 cut(s) 80, 154, 663
PsyI GACNNNGTC 1 cut(s) 848
RsaI GTAC 3 cut(s) 7, 464, 745
RsaNI GTAC 3 cut(s) 6, 463, 744
RseI CAYNNNNRTG 4 cut(s) 284, 535, 857, 895
SacI GAGCTC 1 cut(s) 782
SatI GCNGC 1 cut(s) 379
Sau3AI GATC 3 cut(s) 80, 154, 663
Sau96I GGNCC 3 cut(s) 484, 493, 527
ScaI AGTACT 1 cut(s) 464
ScrFI CCNGG 4 cut(s) 130, 143, 144, 414
SduI GDGCHC 2 cut(s) 552, 782
SfaNI GCATC 1 cut(s) 46
SfcI CTRYAG 3 cut(s) 208, 703, 855
SfoI GGCGCC 1 cut(s) 333
SinI GGWCC 1 cut(s) 484
SmaI CCCGGG 1 cut(s) 144
SmiMI CAYNNNNRTG 4 cut(s) 284, 535, 857, 895
Sse9I AATT 3 cut(s) 51, 761, 784
SsiI CCGC 2 cut(s) 328, 351
SspDI GGCGCC 1 cut(s) 331
SspI AATATT 1 cut(s) 677
SspMI CTAG 3 cut(s) 290, 620, 878
SstI GAGCTC 1 cut(s) 782
StyD4I CCNGG 4 cut(s) 128, 141, 142, 412
StyI CCWWGG 1 cut(s) 403
TaaI ACNGT 1 cut(s) 850
TaqI TCGA 3 cut(s) 151, 318, 788
TasI AATT 3 cut(s) 51, 761, 784
TatI WGTACW 1 cut(s) 462
TfiI GAWTC 2 cut(s) 343, 803
TscAI CASTG 6 cut(s) 576, 596, 613, 859, 864, 870
TseFI GTSAC 3 cut(s) 275, 850, 861
TseI GCWGC 1 cut(s) 378
Tsp45I GTSAC 3 cut(s) 275, 850, 861
TspDTI ATGAA 2 cut(s) 355, 356
TspGWI ACGGA 1 cut(s) 656
TspMI CCCGGG 1 cut(s) 142
TspRI CASTG 6 cut(s) 576, 596, 613, 859, 864, 870
Tth111I GACNNNGTC 1 cut(s) 848
Van91I CCANNNNNTGG 1 cut(s) 502
VpaK11BI GGWCC 1 cut(s) 484
XapI RAATTY 1 cut(s) 784
XceI RCATGY 3 cut(s) 78, 268, 772
XcmI CCANNNNNNNNNTGG 2 cut(s) 131, 503
XmaI CCCGGG 1 cut(s) 142
XmnI GAANNNNTTC 1 cut(s) 439
XspI CTAG 3 cut(s) 290, 620, 878
ZrmI AGTACT 1 cut(s) 464
Zsp2I ATGCAT 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.