Rroxscaffold_3G00262200

Wall-associated receptor kinase galacturonan-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0013565
Physical Location & Seq
Forward (+)
56261249 .. 56262591
1343 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00262200.1

Sequence Viewer

Length: 900 bp
ATGACTACTTCTGCTATGCTCATTCTGAGCTTCCTCCATCTTGTAATTTCAGTACTAATTCCATCAACCTCAGGCAACACATGTAGATCCTACTGTGGAAACATAACCATAGACTACCCCTTTGCCATCCATCCCGGGTGTGGCCACCCCGGTTTTCGAGACCTCCTGTACTGCATCAACGATGTTCTTATGTTCCACATTAGCTCAGGATCCTACAGGGTTCTGGAGATAGACTATGCTTACCAAGCCCTCACTCTACATGACCCTCACATGTCAACTTGTGACACCATTGTGCTAGGTGCAAAGGGTAACGGCTTCGCAGTGGAGCAATGGCGGGCACCGTACATGAATCCGTCCGCCGACAATGTGTTCATGCTGATAGGCTGCTCCGCTCAGTCGCCACTCTTCCAAGGCTTCCCAGGAAAGCACTTGCCATGTAGAAACGTTTCGGGAATGAGCTGTGAGGAGTACTATGGTTGCCCTGCTTGGGACCTTCTGGGTCATAGAATGGTGGGCTCCAAGTTCGGGTCAGGCCCACCCGAGTGTTGTGCTGTGCCTTTTGAATCTATCAAGTCAGTTAACCTCAGCAAGCTTCAGTGTGAAGGATACAGCAGCGCTTACAATCTTGCACCATTGAGGCTTGATGGGGCTAATGGATGGTCTTATGGGATCCGTGTGAAGTATTCTGTTCAAGAAAATGATGAGTTCTGTAGGGCGTGTGAGGCTACCGGCGGCACTTGCGGGTATGGTACCGATGGCATTAGGCAATTATGCATGTGTGGGAGCACAAATTCGACATCAAACTGCGACTCTGTGAAATCATCATCAGGAAGAACATACTCAAACGGAAACACAGTCACAGGATTACTGATGTGTTTGCTAGCTTGGATTGCAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind

Protein Analysis

299

Amino Acids

32.36

Weight (kDa)

6.04

Isoelectric Point (pI)

38.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 28 - 88 3.5e-14 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013565)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50290
fragaria_vesca FvH4_5g05500
malus_domestica MD06G1172100.v1.1 MD14G1178000.v1.1
prunus_persica Prupe.5G171500_v2.0.a1
pyrus_communis pycom06g15330 pycom14g14850
rosa_chinensis RchiOBHm_Chr7g0193341
rosa_laevigata RLG00000004278
rosa_multiflora Rmu_ssc0000092.1_g000009
rosa_roxburghii Rroxscaffold_3G00262200
rosa_rugosa Rorug07G0009000
rosa_samantha Rh7AG134800 Rh7AG135200 Rh7BG135500 Rh7CG138900 Rh7DG136900
rosa_wichuraiana Rw7G011520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 749
AccB1I GGYRCC 2 cut(s) 337, 749
AccBSI CCGCTC 1 cut(s) 392
AciI CCGC 5 cut(s) 334, 357, 390, 732, 741
AclI AACGTT 1 cut(s) 444
AclWI GGATC 5 cut(s) 81, 204, 217, 664, 677
AcoI YGGCCR 1 cut(s) 142
AcsI RAATTY 1 cut(s) 790
AcuI CTGAAG 1 cut(s) 578
AfaI GTAC 5 cut(s) 54, 170, 344, 470, 751
AfeI AGCGCT 1 cut(s) 616
AfiI CCNNNNNNNGG 3 cut(s) 140, 487, 525
AflIII ACRYGT 2 cut(s) 80, 270
AgsI TTSAA 2 cut(s) 563, 692
AjnI CCWGG 1 cut(s) 418
AleI CACNNNNGTG 2 cut(s) 290, 541
AluBI AGCT 5 cut(s) 30, 204, 459, 592, 884
AluI AGCT 5 cut(s) 30, 204, 459, 592, 884
Alw21I GWGCWC 1 cut(s) 788
Alw26I GTCTC 1 cut(s) 153
AlwI GGATC 5 cut(s) 81, 204, 217, 664, 677
Ama87I CYCGRG 2 cut(s) 134, 539
Aor51HI AGCGCT 1 cut(s) 616
AoxI GGCC 2 cut(s) 142, 532
ApeKI GCWGC 2 cut(s) 384, 612
ApoI RAATTY 1 cut(s) 790
ArsI GACNNNNNNTTYG 2 cut(s) 104, 136
Asp700I GAANNNNTTC 1 cut(s) 445
Asp718I GGTACC 1 cut(s) 749
AspLEI GCGC 1 cut(s) 617
AspS9I GGNCC 2 cut(s) 490, 533
AsuC2I CCSGG 3 cut(s) 135, 136, 150
AsuNHI GCTAGC 1 cut(s) 880
AvaI CYCGRG 2 cut(s) 134, 539
AvaII GGWCC 1 cut(s) 490
AxyI CCTNAGG 1 cut(s) 70
BaeGI GKGCMC 1 cut(s) 340
BalI TGGCCA 1 cut(s) 144
BamHI GGATCC 2 cut(s) 209, 669
BanI GGYRCC 2 cut(s) 337, 749
BanII GRGCYC 1 cut(s) 518
Bbv12I GWGCWC 1 cut(s) 788
BbvCI CCTCAGC 1 cut(s) 584
BbvI GCAGC 2 cut(s) 371, 624
BccI CCATC 7 cut(s) 45, 70, 134, 138, 638, 651, 749
BceAI ACGGC 1 cut(s) 328
BcgI CGANNNNNNTGC 2 cut(s) 530, 564
BciT130I CCWGG 1 cut(s) 420
BciVI GTATCC 1 cut(s) 599
BcnI CCSGG 3 cut(s) 135, 136, 150
BcoDI GTCTC 1 cut(s) 153
BfaI CTAG 2 cut(s) 296, 881
BfmI CTRYAG 2 cut(s) 214, 709
BfoI RGCGCY 1 cut(s) 618
BfuI GTATCC 1 cut(s) 599
BisI GCNGC 3 cut(s) 385, 613, 733
BlsI GCNGC 3 cut(s) 386, 614, 734
BmcAI AGTACT 2 cut(s) 54, 470
Bme1390I CCNGG 4 cut(s) 135, 136, 150, 420
Bme18I GGWCC 1 cut(s) 490
BmeT110I CYCGRG 2 cut(s) 134, 539
BmgT120I GGNCC 2 cut(s) 490, 533
BmiI GGNNCC 6 cut(s) 211, 339, 491, 517, 671, 751
BmrFI CCNGG 4 cut(s) 135, 136, 150, 420
BmsI GCATC 1 cut(s) 183
BmtI GCTAGC 1 cut(s) 884
BpmI CTGGAG 1 cut(s) 245
Bpu10I CCTNAGC 2 cut(s) 205, 584
BpuMI CCSGG 3 cut(s) 135, 136, 150
BsaI GGTCTC 1 cut(s) 153
BsaJI CCNNGG 4 cut(s) 134, 148, 409, 418
Bsc4I CCNNNNNNNGG 3 cut(s) 140, 487, 525
Bse118I RCCGGY 1 cut(s) 728
Bse21I CCTNAGG 1 cut(s) 70
Bse3DI GCAATG 1 cut(s) 335
BseBI CCWGG 1 cut(s) 420
BseDI CCNNGG 4 cut(s) 134, 148, 409, 418
BseGI GGATG 3 cut(s) 126, 130, 662
BseLI CCNNNNNNNGG 3 cut(s) 140, 487, 525
BseMI GCAATG 1 cut(s) 335
BseMII CTCAG 5 cut(s) 17, 84, 219, 407, 598
BseRI GAGGAG 1 cut(s) 479
BseSI GKGCMC 1 cut(s) 340
BseXI GCAGC 2 cut(s) 371, 624
BshFI GGCC 2 cut(s) 144, 534
BshNI GGYRCC 2 cut(s) 337, 749
BsiHKAI GWGCWC 1 cut(s) 788
BsiHKCI CYCGRG 2 cut(s) 134, 539
BsiSI CCGG 3 cut(s) 135, 150, 729
BslFI GGGAC 1 cut(s) 503
BslI CCNNNNNNNGG 3 cut(s) 140, 487, 525
BsmAI GTCTC 1 cut(s) 153
BsmFI GGGAC 1 cut(s) 503
BsnI GGCC 2 cut(s) 144, 534
Bso31I GGTCTC 1 cut(s) 153
BsoBI CYCGRG 2 cut(s) 134, 539
Bsp1286I GDGCHC 3 cut(s) 340, 518, 788
Bsp143I GATC 3 cut(s) 86, 209, 669
BspACI CCGC 5 cut(s) 334, 357, 390, 732, 741
BspANI GGCC 2 cut(s) 144, 534
BspCNI CTCAG 5 cut(s) 18, 83, 218, 406, 597
BspLI GGNNCC 6 cut(s) 211, 339, 491, 517, 671, 751
BspOI GCTAGC 1 cut(s) 884
BspPI GGATC 5 cut(s) 81, 204, 217, 664, 677
BspT107I GGYRCC 2 cut(s) 337, 749
BspTNI GGTCTC 1 cut(s) 153
BsrBI CCGCTC 1 cut(s) 392
BsrDI GCAATG 1 cut(s) 335
BsrFI RCCGGY 1 cut(s) 728
BssAI RCCGGY 1 cut(s) 728
BssECI CCNNGG 4 cut(s) 134, 148, 409, 418
BssMI GATC 3 cut(s) 86, 209, 669
BssT1I CCWWGG 1 cut(s) 409
Bst2UI CCWGG 1 cut(s) 420
Bst4CI ACNGT 3 cut(s) 95, 342, 856
Bst6I CTCTTC 1 cut(s) 410
BstC8I GCNNGC 3 cut(s) 336, 590, 882
BstDEI CTNAG 5 cut(s) 26, 70, 205, 393, 584
BstF5I GGATG 3 cut(s) 126, 130, 662
BstH2I RGCGCY 1 cut(s) 618
BstHHI GCGC 1 cut(s) 617
BstKTI GATC 3 cut(s) 89, 212, 672
BstMAI GTCTC 1 cut(s) 153
BstMBI GATC 3 cut(s) 86, 209, 669
BstMWI GCNNNNNNNGC 4 cut(s) 245, 722, 738, 890
BstNI CCWGG 1 cut(s) 420
BstNSI RCATGY 3 cut(s) 84, 274, 778
BstSCI CCNGG 4 cut(s) 133, 134, 148, 418
BstSFI CTRYAG 2 cut(s) 214, 709
BstSLI GKGCMC 1 cut(s) 340
BstV1I GCAGC 2 cut(s) 371, 624
BstX2I RGATCY 3 cut(s) 86, 209, 669
BstYI RGATCY 3 cut(s) 86, 209, 669
Bsu36I CCTNAGG 1 cut(s) 70
BsuI GTATCC 1 cut(s) 599
BsuRI GGCC 2 cut(s) 144, 534
BtsCI GGATG 3 cut(s) 126, 130, 662
BtsI GCAGTG 1 cut(s) 327
BtsIMutI CAGTG 2 cut(s) 327, 602
Cac8I GCNNGC 3 cut(s) 336, 590, 882
CfoI GCGC 1 cut(s) 617
Cfr10I RCCGGY 1 cut(s) 728
Cfr13I GGNCC 2 cut(s) 490, 533
Cfr9I CCCGGG 1 cut(s) 134
Csp6I GTAC 5 cut(s) 53, 169, 343, 469, 750
CviAII CATG 7 cut(s) 81, 260, 271, 346, 373, 435, 775
CviQI GTAC 5 cut(s) 53, 169, 343, 469, 750
DdeI CTNAG 5 cut(s) 26, 70, 205, 393, 584
DpnI GATC 3 cut(s) 88, 211, 671
DpnII GATC 3 cut(s) 86, 209, 669
EaeI YGGCCR 1 cut(s) 142
Eam1104I CTCTTC 1 cut(s) 410
EarI CTCTTC 1 cut(s) 410
EciI GGCGGA 1 cut(s) 346
Eco130I CCWWGG 1 cut(s) 409
Eco24I GRGCYC 1 cut(s) 518
Eco31I GGTCTC 1 cut(s) 153
Eco47I GGWCC 1 cut(s) 490
Eco47III AGCGCT 1 cut(s) 616
Eco57I CTGAAG 1 cut(s) 578
Eco81I CCTNAGG 1 cut(s) 70
Eco88I CYCGRG 2 cut(s) 134, 539
EcoO109I RGGNCCY 1 cut(s) 490
EcoRII CCWGG 1 cut(s) 418
EcoT14I CCWWGG 1 cut(s) 409
EcoT22I ATGCAT 1 cut(s) 776
EcoT38I GRGCYC 1 cut(s) 518
ErhI CCWWGG 1 cut(s) 409
FaeI CATG 7 cut(s) 84, 263, 274, 349, 376, 438, 778
FaqI GGGAC 1 cut(s) 503
FatI CATG 7 cut(s) 80, 259, 270, 345, 372, 434, 774
FauI CCCGC 2 cut(s) 327, 734
Fnu4HI GCNGC 3 cut(s) 385, 613, 733
FokI GGATG 3 cut(s) 113, 117, 669
FriOI GRGCYC 1 cut(s) 518
Fsp4HI GCNGC 3 cut(s) 385, 613, 733
FspBI CTAG 2 cut(s) 296, 881
GlaI GCGC 1 cut(s) 616
GluI GCNGC 3 cut(s) 385, 613, 733
GsuI CTGGAG 1 cut(s) 245
HaeII RGCGCY 1 cut(s) 618
HaeIII GGCC 2 cut(s) 144, 534
HapII CCGG 3 cut(s) 135, 150, 729
HhaI GCGC 1 cut(s) 617
Hin1II CATG 7 cut(s) 84, 263, 274, 349, 376, 438, 778
Hin6I GCGC 1 cut(s) 615
HinP1I GCGC 1 cut(s) 615
HincII GTYRAC 2 cut(s) 276, 580
HindII GTYRAC 2 cut(s) 276, 580
HindIII AAGCTT 1 cut(s) 590
HinfI GANTC 3 cut(s) 349, 563, 809
HpaI GTTAAC 1 cut(s) 580
HpaII CCGG 3 cut(s) 135, 150, 729
Hpy166II GTNNAC 2 cut(s) 276, 580
Hpy188I TCNGA 1 cut(s) 27
Hpy188III TCNNGA 6 cut(s) 158, 207, 224, 450, 692, 828
Hpy8I GTNNAC 2 cut(s) 276, 580
HpyAV CCTTC 2 cut(s) 503, 596
HpyCH4III ACNGT 3 cut(s) 95, 342, 856
HpyCH4IV ACGT 1 cut(s) 444
HpyCH4V TGCA 5 cut(s) 174, 302, 629, 774, 893
HpyF10VI GCNNNNNNNGC 4 cut(s) 245, 722, 738, 890
HpyF3I CTNAG 5 cut(s) 26, 70, 205, 393, 584
HpySE526I ACGT 1 cut(s) 444
Hsp92II CATG 7 cut(s) 84, 263, 274, 349, 376, 438, 778
HspAI GCGC 1 cut(s) 615
KpnI GGTACC 1 cut(s) 753
KspAI GTTAAC 1 cut(s) 580
Kzo9I GATC 3 cut(s) 86, 209, 669
LmnI GCTCC 4 cut(s) 325, 392, 521, 783
Lsp1109I GCAGC 2 cut(s) 371, 624
LweI GCATC 1 cut(s) 183
MaeI CTAG 2 cut(s) 296, 881
MaeII ACGT 1 cut(s) 444
MaeIII GTNAC 3 cut(s) 281, 308, 856
MalI GATC 3 cut(s) 88, 211, 671
MbiI CCGCTC 1 cut(s) 392
MboI GATC 3 cut(s) 86, 209, 669
MboII GAAGA 2 cut(s) 397, 843
MflI RGATCY 3 cut(s) 86, 209, 669
MhlI GDGCHC 3 cut(s) 340, 518, 788
MlsI TGGCCA 1 cut(s) 144
MluCI AATT 5 cut(s) 45, 57, 767, 790, 894
MluNI TGGCCA 1 cut(s) 144
MlyI GAGTC 1 cut(s) 803
MnlI CCTC 9 cut(s) 44, 79, 173, 260, 276, 457, 593, 630, 715
Mox20I TGGCCA 1 cut(s) 144
Mph1103I ATGCAT 1 cut(s) 776
MroXI GAANNNNTTC 1 cut(s) 445
MscI TGGCCA 1 cut(s) 144
MseI TTAA 1 cut(s) 579
MslI CAYNNNNRTG 2 cut(s) 290, 541
Msp20I TGGCCA 1 cut(s) 144
MspI CCGG 3 cut(s) 135, 150, 729
MspR9I CCNGG 4 cut(s) 135, 136, 150, 420
MvaI CCWGG 1 cut(s) 420
MwoI GCNNNNNNNGC 4 cut(s) 245, 722, 738, 890
NciI CCSGG 3 cut(s) 135, 136, 150
NdeII GATC 3 cut(s) 86, 209, 669
NheI GCTAGC 1 cut(s) 880
NlaIII CATG 7 cut(s) 84, 263, 274, 349, 376, 438, 778
NlaIV GGNNCC 6 cut(s) 211, 339, 491, 517, 671, 751
NmuCI GTSAC 2 cut(s) 281, 856
NsiI ATGCAT 1 cut(s) 776
NspI RCATGY 3 cut(s) 84, 274, 778
OliI CACNNNNGTG 2 cut(s) 290, 541
PciI ACATGT 2 cut(s) 80, 270
PdmI GAANNNNTTC 1 cut(s) 445
PfeI GAWTC 2 cut(s) 349, 563
PkrI GCNGC 3 cut(s) 386, 614, 734
PleI GAGTC 1 cut(s) 803
PpsI GAGTC 1 cut(s) 803
PpuMI RGGWCCY 1 cut(s) 490
PscI ACATGT 2 cut(s) 80, 270
Psp1406I AACGTT 1 cut(s) 444
Psp5II RGGWCCY 1 cut(s) 490
Psp6I CCWGG 1 cut(s) 418
PspGI CCWGG 1 cut(s) 418
PspN4I GGNNCC 6 cut(s) 211, 339, 491, 517, 671, 751
PspPI GGNCC 2 cut(s) 490, 533
PspPPI RGGWCCY 1 cut(s) 490
PsuI RGATCY 3 cut(s) 86, 209, 669
RsaI GTAC 5 cut(s) 54, 170, 344, 470, 751
RsaNI GTAC 5 cut(s) 53, 169, 343, 469, 750
RseI CAYNNNNRTG 2 cut(s) 290, 541
SaqAI TTAA 1 cut(s) 579
SatI GCNGC 3 cut(s) 385, 613, 733
Sau3AI GATC 3 cut(s) 86, 209, 669
Sau96I GGNCC 2 cut(s) 490, 533
ScaI AGTACT 2 cut(s) 54, 470
SchI GAGTC 1 cut(s) 803
ScrFI CCNGG 4 cut(s) 135, 136, 150, 420
SduI GDGCHC 3 cut(s) 340, 518, 788
SfaNI GCATC 1 cut(s) 183
SfcI CTRYAG 2 cut(s) 214, 709
SinI GGWCC 1 cut(s) 490
SmaI CCCGGG 1 cut(s) 136
SmiMI CAYNNNNRTG 2 cut(s) 290, 541
Sse9I AATT 5 cut(s) 45, 57, 767, 790, 894
SsiI CCGC 5 cut(s) 334, 357, 390, 732, 741
SspMI CTAG 2 cut(s) 296, 881
StyD4I CCNGG 4 cut(s) 133, 134, 148, 418
StyI CCWWGG 1 cut(s) 409
TaaI ACNGT 3 cut(s) 95, 342, 856
TaiI ACGT 1 cut(s) 447
TaqI TCGA 2 cut(s) 157, 794
TasI AATT 5 cut(s) 45, 57, 767, 790, 894
TatI WGTACW 3 cut(s) 52, 168, 468
TauI GCSGC 1 cut(s) 735
TfiI GAWTC 2 cut(s) 349, 563
Tru1I TTAA 1 cut(s) 579
Tru9I TTAA 1 cut(s) 579
TscAI CASTG 2 cut(s) 327, 602
TseFI GTSAC 2 cut(s) 281, 856
TseI GCWGC 2 cut(s) 384, 612
Tsp45I GTSAC 2 cut(s) 281, 856
TspDTI ATGAA 2 cut(s) 361, 362
TspGWI ACGGA 3 cut(s) 342, 662, 861
TspMI CCCGGG 1 cut(s) 134
TspRI CASTG 2 cut(s) 327, 602
VpaK11BI GGWCC 1 cut(s) 490
XapI RAATTY 1 cut(s) 790
XceI RCATGY 3 cut(s) 84, 274, 778
XcmI CCANNNNNNNNNTGG 1 cut(s) 137
XmaI CCCGGG 1 cut(s) 134
XmnI GAANNNNTTC 1 cut(s) 445
XspI CTAG 2 cut(s) 296, 881
ZrmI AGTACT 2 cut(s) 54, 470
Zsp2I ATGCAT 1 cut(s) 776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.