pycom14g14850

Wall-associated receptor kinase galacturonan-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0013565
Physical Location & Seq
Reverse (-)
17529095 .. 17530190
1096 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g14850.2

Sequence Viewer

Length: 906 bp
ATGAGTAGCAATACTACAACAACTATGCTCATAATTTCCAGCTTCCTCTGTCTTATATTTTCTTCGTTTCTTCCATCAAGTTCGGCCAACACCTGCAGATCGTATTGCGGAAACCTCACCGTAGATTACCCGTTCGCCGTCCACTCCGGCTGCGGCCACCCGGGCTTTCGAGATCTCTTATACTGCATCAACGATGTGCTCATGTTTCACATCAGCTCGGGATCTTACAGAGTCTTGGACATAGACTACGCTTACCAAGCCCTCACGTTACACGACCCTCACATGTCAACATGCGACAACATAGTGCTAGGTGCCAAGGGCAACGGCTTCTCCGTCGAGCAATGGCGGGCCCCGTACATGACCCCGACCACCGACAACGTGTTCATGCTGATCGGCTGCTCGGCTGAGTCGCCGCTCTTCCAAGGCTTTCCGGGGAAGCACTTGCCATGCAGAAACGTTTCCGGCATGAGTTGCGAGGAGTACTATGGTTGTCCAGCGTGGAATTCGTTGGGCCATAGGAGGGTGGGCTTCTGGTTTGGGTCGGGCCCACCTGAGTGCTGTGCAGTGCCGTTTGAGGCCATCAAGGCCGTTAATCTCACCAAGCTTCAGTGTCAAGGGTATAGCAGTGCTTACAATCTGGCCCCACTGAAGCTCGATGGGGCCCACGGGTGGTCTTATGGTATCCGAGTGAAGTATTCTGTTCAAGAAAATGATCAGTTTTGTAGAGCGTGTGAGGCAACCGGAGGCACGTGCGGTTACGGTACCGATGGCATTAAGCAATTGTGTATGTGTGGGAGCTTCAATTCCACATCAAATTGTGATTCCGTAAAATCGGCATCTACAAGAACATACCCTATGGTGGAGAGTTACACATTGAGAGGTACTTTCTTCTGCTCATCATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind

Protein Analysis

302

Amino Acids

32.91

Weight (kDa)

6.42

Isoelectric Point (pI)

42.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013565)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50290
fragaria_vesca FvH4_5g05500
malus_domestica MD06G1172100.v1.1 MD14G1178000.v1.1
prunus_persica Prupe.5G171500_v2.0.a1
pyrus_communis pycom06g15330 pycom14g14850
rosa_chinensis RchiOBHm_Chr7g0193341
rosa_laevigata RLG00000004278
rosa_multiflora Rmu_ssc0000092.1_g000009
rosa_roxburghii Rroxscaffold_3G00262200
rosa_rugosa Rorug07G0009000
rosa_samantha Rh7AG134800 Rh7AG135200 Rh7BG135500 Rh7CG138900 Rh7DG136900
rosa_wichuraiana Rw7G011520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 101
Acc36I ACCTGC 1 cut(s) 101
Acc65I GGTACC 1 cut(s) 761
AccB1I GGYRCC 2 cut(s) 311, 761
AccBSI CCGCTC 1 cut(s) 415
AciI CCGC 5 cut(s) 108, 153, 346, 413, 753
AclI AACGTT 1 cut(s) 456
AclWI GGATC 1 cut(s) 229
AcoI YGGCCR 2 cut(s) 84, 154
AcsI RAATTY 1 cut(s) 502
AcuI CTGAAG 2 cut(s) 590, 668
AcvI CACGTG 1 cut(s) 750
AfaI GTAC 4 cut(s) 356, 482, 763, 883
AfiI CCNNNNNNNGG 3 cut(s) 520, 669, 859
AflIII ACRYGT 2 cut(s) 282, 378
AgsI TTSAA 2 cut(s) 704, 802
AleI CACNNNNGTG 1 cut(s) 553
AluBI AGCT 5 cut(s) 42, 216, 604, 652, 798
AluI AGCT 5 cut(s) 42, 216, 604, 652, 798
Alw21I GWGCWC 1 cut(s) 201
AlwI GGATC 1 cut(s) 229
Ama87I CYCGRG 2 cut(s) 160, 217
AoxI GGCC 9 cut(s) 84, 154, 348, 511, 544, 576, 585, 639, 660
ApaI GGGCCC 3 cut(s) 352, 548, 664
ApeKI GCWGC 2 cut(s) 150, 396
ApoI RAATTY 1 cut(s) 502
Asp700I GAANNNNTTC 1 cut(s) 457
Asp718I GGTACC 1 cut(s) 761
AspS9I GGNCC 8 cut(s) 348, 349, 511, 544, 545, 640, 660, 661
AsuC2I CCSGG 3 cut(s) 161, 162, 432
AsuHPI GGTGA 2 cut(s) 109, 589
AvaI CYCGRG 2 cut(s) 160, 217
BaeGI GKGCMC 3 cut(s) 352, 548, 664
BanI GGYRCC 2 cut(s) 311, 761
BanII GRGCYC 3 cut(s) 352, 548, 664
BbrPI CACGTG 1 cut(s) 750
Bbv12I GWGCWC 1 cut(s) 201
BbvI GCAGC 2 cut(s) 137, 383
BccI CCATC 4 cut(s) 82, 587, 650, 761
BceAI ACGGC 4 cut(s) 122, 340, 553, 572
BciVI GTATCC 1 cut(s) 692
BclI TGATCA 1 cut(s) 712
BcnI CCSGG 3 cut(s) 161, 162, 432
BfaI CTAG 1 cut(s) 308
BfmI CTRYAG 1 cut(s) 94
BfuAI ACCTGC 1 cut(s) 101
BfuI GTATCC 1 cut(s) 692
BglI GCCNNNNNGGC 2 cut(s) 162, 584
BglII AGATCT 1 cut(s) 172
BisI GCNGC 4 cut(s) 151, 154, 397, 413
BlsI GCNGC 4 cut(s) 152, 155, 398, 414
BmcAI AGTACT 1 cut(s) 482
Bme1390I CCNGG 3 cut(s) 161, 162, 432
BmeT110I CYCGRG 2 cut(s) 160, 217
BmgT120I GGNCC 8 cut(s) 348, 349, 511, 544, 545, 640, 660, 661
BmiI GGNNCC 8 cut(s) 313, 350, 351, 546, 642, 661, 662, 763
BmrFI CCNGG 3 cut(s) 161, 162, 432
BmsI GCATC 2 cut(s) 195, 845
BpuMI CCSGG 3 cut(s) 161, 162, 432
BsaAI YACGTR 1 cut(s) 750
BsaJI CCNNGG 5 cut(s) 160, 315, 421, 431, 664
BsaWI WCCGGW 1 cut(s) 740
BsaXI ACNNNNNCTCC 2 cut(s) 314, 344
Bsc4I CCNNNNNNNGG 3 cut(s) 520, 669, 859
Bse3DI GCAATG 1 cut(s) 347
BseDI CCNNGG 5 cut(s) 160, 315, 421, 431, 664
BseLI CCNNNNNNNGG 3 cut(s) 520, 669, 859
BseMI GCAATG 1 cut(s) 347
BseMII CTCAG 2 cut(s) 396, 543
BseRI GAGGAG 1 cut(s) 491
BseSI GKGCMC 3 cut(s) 352, 548, 664
BseXI GCAGC 2 cut(s) 137, 383
BsgI GTGCAG 1 cut(s) 582
BshFI GGCC 9 cut(s) 86, 156, 350, 513, 546, 578, 587, 641, 662
BshNI GGYRCC 2 cut(s) 311, 761
BsiHKAI GWGCWC 1 cut(s) 201
BsiHKCI CYCGRG 2 cut(s) 160, 217
BsiSI CCGG 5 cut(s) 147, 161, 431, 462, 741
BslI CCNNNNNNNGG 3 cut(s) 520, 669, 859
BsnI GGCC 9 cut(s) 86, 156, 350, 513, 546, 578, 587, 641, 662
BsoBI CYCGRG 2 cut(s) 160, 217
Bsp120I GGGCCC 3 cut(s) 348, 544, 660
Bsp1286I GDGCHC 4 cut(s) 201, 352, 548, 664
Bsp143I GATC 5 cut(s) 98, 172, 221, 390, 712
BspACI CCGC 5 cut(s) 108, 153, 346, 413, 753
BspANI GGCC 9 cut(s) 86, 156, 350, 513, 546, 578, 587, 641, 662
BspCNI CTCAG 2 cut(s) 397, 544
BspLI GGNNCC 8 cut(s) 313, 350, 351, 546, 642, 661, 662, 763
BspMAI CTGCAG 1 cut(s) 98
BspMI ACCTGC 1 cut(s) 101
BspPI GGATC 1 cut(s) 229
BspQI GCTCTTC 1 cut(s) 422
BspT107I GGYRCC 2 cut(s) 311, 761
BsrBI CCGCTC 1 cut(s) 415
BsrDI GCAATG 1 cut(s) 347
BssECI CCNNGG 5 cut(s) 160, 315, 421, 431, 664
BssMI GATC 5 cut(s) 98, 172, 221, 390, 712
BssT1I CCWWGG 2 cut(s) 315, 421
Bst4CI ACNGT 2 cut(s) 121, 761
Bst6I CTCTTC 1 cut(s) 422
BstAPI GCANNNNNTGC 1 cut(s) 471
BstBAI YACGTR 1 cut(s) 750
BstC8I GCNNGC 1 cut(s) 348
BstDEI CTNAG 2 cut(s) 405, 552
BstDSI CCRYGG 1 cut(s) 664
BstKTI GATC 5 cut(s) 101, 175, 224, 393, 715
BstMBI GATC 5 cut(s) 98, 172, 221, 390, 712
BstMWI GCNNNNNNNGC 5 cut(s) 162, 257, 471, 584, 734
BstNSI RCATGY 2 cut(s) 286, 294
BstSCI CCNGG 3 cut(s) 159, 160, 430
BstSFI CTRYAG 1 cut(s) 94
BstSLI GKGCMC 3 cut(s) 352, 548, 664
BstV1I GCAGC 2 cut(s) 137, 383
BstX2I RGATCY 2 cut(s) 172, 221
BstYI RGATCY 2 cut(s) 172, 221
BsuI GTATCC 1 cut(s) 692
BsuRI GGCC 9 cut(s) 86, 156, 350, 513, 546, 578, 587, 641, 662
BtgI CCRYGG 1 cut(s) 664
BtsI GCAGTG 2 cut(s) 570, 631
BtsIMutI CAGTG 4 cut(s) 570, 614, 631, 644
BveI ACCTGC 1 cut(s) 101
Cac8I GCNNGC 1 cut(s) 348
Cfr13I GGNCC 8 cut(s) 348, 349, 511, 544, 545, 640, 660, 661
Cfr9I CCCGGG 1 cut(s) 160
Csp6I GTAC 4 cut(s) 355, 481, 762, 882
CviAII CATG 7 cut(s) 202, 283, 291, 358, 385, 447, 466
CviQI GTAC 4 cut(s) 355, 481, 762, 882
DdeI CTNAG 2 cut(s) 405, 552
DpnI GATC 5 cut(s) 100, 174, 223, 392, 714
DpnII GATC 5 cut(s) 98, 172, 221, 390, 712
EaeI YGGCCR 2 cut(s) 84, 154
Eam1104I CTCTTC 1 cut(s) 422
EarI CTCTTC 1 cut(s) 422
Eco130I CCWWGG 2 cut(s) 315, 421
Eco24I GRGCYC 3 cut(s) 352, 548, 664
Eco57I CTGAAG 2 cut(s) 590, 668
Eco72I CACGTG 1 cut(s) 750
Eco88I CYCGRG 2 cut(s) 160, 217
EcoO109I RGGNCCY 2 cut(s) 349, 660
EcoRI GAATTC 1 cut(s) 502
EcoT14I CCWWGG 2 cut(s) 315, 421
EcoT38I GRGCYC 3 cut(s) 352, 548, 664
ErhI CCWWGG 2 cut(s) 315, 421
FaeI CATG 7 cut(s) 205, 286, 294, 361, 388, 450, 469
FatI CATG 7 cut(s) 201, 282, 290, 357, 384, 446, 465
FauI CCCGC 1 cut(s) 339
FbaI TGATCA 1 cut(s) 712
Fnu4HI GCNGC 4 cut(s) 151, 154, 397, 413
FriOI GRGCYC 3 cut(s) 352, 548, 664
Fsp4HI GCNGC 4 cut(s) 151, 154, 397, 413
FspBI CTAG 1 cut(s) 308
GluI GCNGC 4 cut(s) 151, 154, 397, 413
HaeIII GGCC 9 cut(s) 86, 156, 350, 513, 546, 578, 587, 641, 662
HapII CCGG 5 cut(s) 147, 161, 431, 462, 741
Hin1II CATG 7 cut(s) 205, 286, 294, 361, 388, 450, 469
HincII GTYRAC 1 cut(s) 288
HindII GTYRAC 1 cut(s) 288
HindIII AAGCTT 1 cut(s) 602
HinfI GANTC 3 cut(s) 231, 407, 821
HpaII CCGG 5 cut(s) 147, 161, 431, 462, 741
HphI GGTGA 2 cut(s) 109, 589
Hpy166II GTNNAC 2 cut(s) 142, 288
Hpy188I TCNGA 1 cut(s) 686
Hpy188III TCNNGA 3 cut(s) 170, 219, 704
Hpy8I GTNNAC 2 cut(s) 142, 288
Hpy99I CGWCG 1 cut(s) 338
HpyCH4III ACNGT 2 cut(s) 121, 761
HpyCH4IV ACGT 4 cut(s) 266, 378, 456, 749
HpyCH4V TGCA 4 cut(s) 96, 186, 450, 563
HpyF10VI GCNNNNNNNGC 5 cut(s) 162, 257, 471, 584, 734
HpyF3I CTNAG 2 cut(s) 405, 552
HpySE526I ACGT 4 cut(s) 266, 378, 456, 749
Hsp92II CATG 7 cut(s) 205, 286, 294, 361, 388, 450, 469
KpnI GGTACC 1 cut(s) 765
Ksp22I TGATCA 1 cut(s) 712
Kzo9I GATC 5 cut(s) 98, 172, 221, 390, 712
LguI GCTCTTC 1 cut(s) 422
LmnI GCTCC 1 cut(s) 795
Lsp1109I GCAGC 2 cut(s) 137, 383
LweI GCATC 2 cut(s) 195, 845
MaeI CTAG 1 cut(s) 308
MaeII ACGT 4 cut(s) 266, 378, 456, 749
MaeIII GTNAC 3 cut(s) 267, 755, 866
MalI GATC 5 cut(s) 100, 174, 223, 392, 714
MbiI CCGCTC 1 cut(s) 415
MboI GATC 5 cut(s) 98, 172, 221, 390, 712
MboII GAAGA 4 cut(s) 54, 62, 409, 880
MfeI CAATTG 1 cut(s) 779
MflI RGATCY 2 cut(s) 172, 221
MhlI GDGCHC 4 cut(s) 201, 352, 548, 664
MluCI AATT 5 cut(s) 33, 502, 779, 802, 814
MlyI GAGTC 2 cut(s) 240, 416
MroXI GAANNNNTTC 1 cut(s) 457
MseI TTAA 2 cut(s) 591, 774
MslI CAYNNNNRTG 1 cut(s) 553
MspI CCGG 5 cut(s) 147, 161, 431, 462, 741
MspR9I CCNGG 3 cut(s) 161, 162, 432
MunI CAATTG 1 cut(s) 779
MwoI GCNNNNNNNGC 5 cut(s) 162, 257, 471, 584, 734
NciI CCSGG 3 cut(s) 161, 162, 432
NdeII GATC 5 cut(s) 98, 172, 221, 390, 712
NlaIII CATG 7 cut(s) 205, 286, 294, 361, 388, 450, 469
NlaIV GGNNCC 8 cut(s) 313, 350, 351, 546, 642, 661, 662, 763
NmeAIII GCCGAG 1 cut(s) 380
NspI RCATGY 2 cut(s) 286, 294
OliI CACNNNNGTG 1 cut(s) 553
PaqCI CACCTGC 1 cut(s) 101
PciI ACATGT 1 cut(s) 282
PciSI GCTCTTC 1 cut(s) 422
PcsI WCGNNNNNNNCGW 1 cut(s) 330
PdmI GAANNNNTTC 1 cut(s) 457
PfeI GAWTC 1 cut(s) 821
PkrI GCNGC 4 cut(s) 152, 155, 398, 414
PleI GAGTC 2 cut(s) 239, 415
PmaCI CACGTG 1 cut(s) 750
PmlI CACGTG 1 cut(s) 750
PpsI GAGTC 2 cut(s) 239, 415
Ppu21I YACGTR 1 cut(s) 750
PscI ACATGT 1 cut(s) 282
Psp1406I AACGTT 1 cut(s) 456
PspCI CACGTG 1 cut(s) 750
PspN4I GGNNCC 8 cut(s) 313, 350, 351, 546, 642, 661, 662, 763
PspOMI GGGCCC 3 cut(s) 348, 544, 660
PspPI GGNCC 8 cut(s) 348, 349, 511, 544, 545, 640, 660, 661
PstI CTGCAG 1 cut(s) 98
PsuI RGATCY 2 cut(s) 172, 221
RsaI GTAC 4 cut(s) 356, 482, 763, 883
RsaNI GTAC 4 cut(s) 355, 481, 762, 882
RseI CAYNNNNRTG 1 cut(s) 553
SapI GCTCTTC 1 cut(s) 422
SaqAI TTAA 2 cut(s) 591, 774
SatI GCNGC 4 cut(s) 151, 154, 397, 413
Sau3AI GATC 5 cut(s) 98, 172, 221, 390, 712
Sau96I GGNCC 8 cut(s) 348, 349, 511, 544, 545, 640, 660, 661
ScaI AGTACT 1 cut(s) 482
SchI GAGTC 2 cut(s) 240, 416
ScrFI CCNGG 3 cut(s) 161, 162, 432
SduI GDGCHC 4 cut(s) 201, 352, 548, 664
SfaNI GCATC 2 cut(s) 195, 845
SfcI CTRYAG 1 cut(s) 94
SfiI GGCCNNNNNGGCC 1 cut(s) 584
SmaI CCCGGG 1 cut(s) 162
SmiMI CAYNNNNRTG 1 cut(s) 553
Sse9I AATT 5 cut(s) 33, 502, 779, 802, 814
SsiI CCGC 5 cut(s) 108, 153, 346, 413, 753
SspMI CTAG 1 cut(s) 308
StyD4I CCNGG 3 cut(s) 159, 160, 430
StyI CCWWGG 2 cut(s) 315, 421
TaaI ACNGT 2 cut(s) 121, 761
TaiI ACGT 4 cut(s) 269, 381, 459, 752
TaqI TCGA 3 cut(s) 169, 336, 654
TasI AATT 5 cut(s) 33, 502, 779, 802, 814
TatI WGTACW 1 cut(s) 480
TauI GCSGC 2 cut(s) 156, 415
TfiI GAWTC 1 cut(s) 821
Tru1I TTAA 2 cut(s) 591, 774
Tru9I TTAA 2 cut(s) 591, 774
TscAI CASTG 4 cut(s) 570, 614, 631, 651
TseI GCWGC 2 cut(s) 150, 396
TspDTI ATGAA 1 cut(s) 373
TspGWI ACGGA 2 cut(s) 322, 814
TspMI CCCGGG 1 cut(s) 160
TspRI CASTG 4 cut(s) 570, 614, 631, 651
XapI RAATTY 1 cut(s) 502
XceI RCATGY 2 cut(s) 286, 294
XmaI CCCGGG 1 cut(s) 160
XmnI GAANNNNTTC 1 cut(s) 457
XspI CTAG 1 cut(s) 308
ZrmI AGTACT 1 cut(s) 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.