pycom06g15330

Wall-associated receptor kinase galacturonan-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0013565
Physical Location & Seq
Reverse (-)
19878966 .. 19880588
1623 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g15330.2

Sequence Viewer

Length: 1224 bp
ATGGACAAGCAAAGACGTTGGAACATAAAGCAACCCCATCGGAATCATTTCGTGCAATTTTATTGCCGTCCCAAGACCCAAAAGCAAACTAAAGCTTCTGCAGGATTTCAATTTTTCAAAAGAGCAAAACCCATTAACCATTCACCAAAAGATATTCTCAACACAAATCATCCAAGCAAGGTATCAATATCATCTGCGTTTTCCACTGTCACTCATATCACAAACCACACAGCTGACTCAAAACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCGCATCCATGAGTGGCTATAGTACTACTACTATGCTCATAATTCCCAGCTTCCTCCTCTGTCTTTTATTTTCTTCGTTCTTTCCATCCGGCTCTACCAACACATGCAGATCGTATTGCGGAAACCTCACCATAGATTACCCGTTCGCCGTCCGCTCCGGCTGCGGGCACCCGGGTTTTCGAGATCTCTTATACTGCATCAACGATGTGCTCATGTTTCACATAAGCTCGGGATCTTACAGGGTCTTGGACATAGACTACGCTTACCAAGCCCTCACGTTGCATGAACCTCACATGTCAACATGTGACAGCATAGTGCTAGGTGCCAAGGGCAATGGCTTCTCCGTCGAGCAATGGCGGGCTCCGTACATGAATCCGACCGCCGACAACGTGTTCATGCTGATCGGCTGCTCCGCTCAGTCGCCGCTCTTTCAAGGCTTTCCGGGGAAGCACTTGCCATGCAGAAACGTTTCGGGAATGAGTTGCGAGGAATACTATGGTTGTCCGGCGTGGGATATGTTGGGCCATAGGAGGGTGGGTTCCATGTTTGGTTCGGGTCCACCTGAGTGTTGTGCAGTGCCGTTTGAGGCCATCAAGGCCGTTAATCTCACCAAGCTTCAGTGTGAAGGGTATAGCAGTGCTTACAACCTGGCCCCGCTGAAGCTGGATGGGGCCCATGGGTGGTCGTATGGTATCCGAGTGAAGTACTCTGTTCAAGAAAACGATGAGTTTTGTAGAGCTTGTGAGGCTACCAGTGGCACGTGCGGGTACGGCGCTGATGGCATTAGGCAATTGTGTATGTGTGGGAGCTTCAATTCCACGTCAAATTGTGATTCTGTAAAATCAGCATCAAAAAGAACATACTCTATGGCAGAGAGTTATACATTCAGAGGATTGCTGGTGTGTCTACTAGCTTATATTACACAGAAACATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind

Protein Analysis

408

Amino Acids

45.03

Weight (kDa)

8.77

Isoelectric Point (pI)

44.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013565)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50290
fragaria_vesca FvH4_5g05500
malus_domestica MD06G1172100.v1.1 MD14G1178000.v1.1
prunus_persica Prupe.5G171500_v2.0.a1
pyrus_communis pycom06g15330 pycom14g14850
rosa_chinensis RchiOBHm_Chr7g0193341
rosa_laevigata RLG00000004278
rosa_multiflora Rmu_ssc0000092.1_g000009
rosa_roxburghii Rroxscaffold_3G00262200
rosa_rugosa Rorug07G0009000
rosa_samantha Rh7AG134800 Rh7AG135200 Rh7BG135500 Rh7CG138900 Rh7DG136900
rosa_wichuraiana Rw7G011520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 453, 608
AccBSI CCGCTC 3 cut(s) 441, 701, 712
AccI GTMKAC 1 cut(s) 1192
AciI CCGC 9 cut(s) 405, 439, 450, 643, 666, 699, 710, 941, 1050
AclI AACGTT 1 cut(s) 753
AclWI GGATC 1 cut(s) 526
AcuI CTGAAG 2 cut(s) 887, 965
AcvI CACGTG 1 cut(s) 1047
AfaI GTAC 4 cut(s) 310, 653, 992, 1055
AfiI CCNNNNNNNGG 3 cut(s) 450, 817, 966
AflIII ACRYGT 3 cut(s) 579, 587, 675
AgsI TTSAA 5 cut(s) 110, 118, 719, 1001, 1099
AjiI CACGTC 1 cut(s) 1107
AjnI CCWGG 1 cut(s) 933
AleI CACNNNNGTG 1 cut(s) 850
AloI GAACNNNNNNTCC 2 cut(s) 808, 840
AluBI AGCT 9 cut(s) 95, 233, 336, 513, 901, 949, 1025, 1095, 1199
AluI AGCT 9 cut(s) 95, 233, 336, 513, 901, 949, 1025, 1095, 1199
Alw21I GWGCWC 1 cut(s) 498
AlwI GGATC 1 cut(s) 526
Ama87I CYCGRG 2 cut(s) 457, 514
AoxI GGCC 5 cut(s) 808, 873, 882, 936, 957
ApaI GGGCCC 1 cut(s) 961
ApeKI GCWGC 2 cut(s) 447, 693
Asp700I GAANNNNTTC 2 cut(s) 47, 754
AspLEI GCGC 1 cut(s) 1061
AspS9I GGNCC 5 cut(s) 808, 842, 937, 957, 958
AsuC2I CCSGG 3 cut(s) 458, 459, 729
AsuHPI GGTGA 3 cut(s) 135, 406, 886
AvaI CYCGRG 2 cut(s) 457, 514
AvaII GGWCC 1 cut(s) 842
BaeGI GKGCMC 2 cut(s) 456, 961
BanI GGYRCC 2 cut(s) 453, 608
BanII GRGCYC 2 cut(s) 649, 961
BbrPI CACGTG 1 cut(s) 1047
Bbv12I GWGCWC 1 cut(s) 498
BbvI GCAGC 2 cut(s) 434, 680
BccI CCATC 5 cut(s) 45, 379, 884, 947, 1058
BceAI ACGGC 5 cut(s) 51, 419, 850, 869, 1072
BcgI CGANNNNNNTGC 2 cut(s) 20, 54
BciT130I CCWGG 1 cut(s) 935
BciVI GTATCC 1 cut(s) 989
BcnI CCSGG 3 cut(s) 458, 459, 729
BfaI CTAG 2 cut(s) 605, 1196
BfmI CTRYAG 2 cut(s) 99, 304
BfoI RGCGCY 1 cut(s) 1062
BfuI GTATCC 1 cut(s) 989
BglI GCCNNNNNGGC 1 cut(s) 881
BglII AGATCT 1 cut(s) 469
BisI GCNGC 3 cut(s) 448, 694, 710
BlsI GCNGC 3 cut(s) 449, 695, 711
BmcAI AGTACT 2 cut(s) 310, 992
Bme1390I CCNGG 4 cut(s) 458, 459, 729, 935
Bme18I GGWCC 1 cut(s) 842
BmeT110I CYCGRG 2 cut(s) 457, 514
BmgBI CACGTC 1 cut(s) 1107
BmgT120I GGNCC 5 cut(s) 808, 842, 937, 957, 958
BmiI GGNNCC 8 cut(s) 455, 610, 648, 826, 843, 939, 958, 959
BmrFI CCNGG 4 cut(s) 458, 459, 729, 935
BmsI GCATC 3 cut(s) 299, 492, 1142
BpuMI CCSGG 3 cut(s) 458, 459, 729
BsaAI YACGTR 1 cut(s) 1047
BsaJI CCNNGG 4 cut(s) 457, 612, 728, 961
BsaXI ACNNNNNCTCC 2 cut(s) 808, 838
Bsc4I CCNNNNNNNGG 3 cut(s) 450, 817, 966
Bse1I ACTGG 1 cut(s) 1038
Bse3DI GCAATG 2 cut(s) 625, 644
BseBI CCWGG 1 cut(s) 935
BseDI CCNNGG 4 cut(s) 457, 612, 728, 961
BseGI GGATG 4 cut(s) 169, 290, 371, 958
BseLI CCNNNNNNNGG 3 cut(s) 450, 817, 966
BseMI GCAATG 2 cut(s) 625, 644
BseMII CTCAG 2 cut(s) 716, 840
BseNI ACTGG 1 cut(s) 1038
BseRI GAGGAG 1 cut(s) 332
BseSI GKGCMC 2 cut(s) 456, 961
BseXI GCAGC 2 cut(s) 434, 680
BseYI CCCAGC 1 cut(s) 332
BsgI GTGCAG 1 cut(s) 879
Bsh1285I CGRYCG 1 cut(s) 666
BshFI GGCC 5 cut(s) 810, 875, 884, 938, 959
BshNI GGYRCC 2 cut(s) 453, 608
BsiEI CGRYCG 1 cut(s) 666
BsiHKAI GWGCWC 1 cut(s) 498
BsiHKCI CYCGRG 2 cut(s) 457, 514
BsiSI CCGG 5 cut(s) 375, 444, 458, 728, 791
BslFI GGGAC 1 cut(s) 54
BslI CCNNNNNNNGG 3 cut(s) 450, 817, 966
BsmFI GGGAC 1 cut(s) 54
BsnI GGCC 5 cut(s) 810, 875, 884, 938, 959
BsoBI CYCGRG 2 cut(s) 457, 514
Bsp120I GGGCCC 1 cut(s) 957
Bsp1286I GDGCHC 4 cut(s) 456, 498, 649, 961
Bsp143I GATC 4 cut(s) 395, 469, 518, 687
Bsp19I CCATGG 1 cut(s) 961
BspACI CCGC 9 cut(s) 405, 439, 450, 643, 666, 699, 710, 941, 1050
BspANI GGCC 5 cut(s) 810, 875, 884, 938, 959
BspCNI CTCAG 2 cut(s) 715, 841
BspLI GGNNCC 8 cut(s) 455, 610, 648, 826, 843, 939, 958, 959
BspMAI CTGCAG 1 cut(s) 103
BspPI GGATC 1 cut(s) 526
BspT107I GGYRCC 2 cut(s) 453, 608
BsrBI CCGCTC 3 cut(s) 441, 701, 712
BsrDI GCAATG 2 cut(s) 625, 644
BsrI ACTGG 1 cut(s) 1038
BssECI CCNNGG 4 cut(s) 457, 612, 728, 961
BssMI GATC 4 cut(s) 395, 469, 518, 687
BssT1I CCWWGG 2 cut(s) 612, 961
Bst2UI CCWGG 1 cut(s) 935
Bst4CI ACNGT 1 cut(s) 208
BstBAI YACGTR 1 cut(s) 1047
BstC8I GCNNGC 2 cut(s) 452, 645
BstDEI CTNAG 2 cut(s) 702, 849
BstDSI CCRYGG 1 cut(s) 961
BstF5I GGATG 4 cut(s) 169, 290, 371, 958
BstH2I RGCGCY 1 cut(s) 1062
BstHHI GCGC 1 cut(s) 1061
BstKTI GATC 4 cut(s) 398, 472, 521, 690
BstMBI GATC 4 cut(s) 395, 469, 518, 687
BstMCI CGRYCG 1 cut(s) 666
BstMWI GCNNNNNNNGC 6 cut(s) 447, 554, 881, 1031, 1056, 1065
BstNI CCWGG 1 cut(s) 935
BstNSI RCATGY 3 cut(s) 393, 583, 591
BstSCI CCNGG 4 cut(s) 456, 457, 727, 933
BstSFI CTRYAG 2 cut(s) 99, 304
BstSLI GKGCMC 2 cut(s) 456, 961
BstV1I GCAGC 2 cut(s) 434, 680
BstX2I RGATCY 2 cut(s) 469, 518
BstYI RGATCY 2 cut(s) 469, 518
BsuI GTATCC 1 cut(s) 989
BsuRI GGCC 5 cut(s) 810, 875, 884, 938, 959
BtgI CCRYGG 1 cut(s) 961
BtrI CACGTC 1 cut(s) 1107
BtsCI GGATG 4 cut(s) 169, 290, 371, 958
BtsI GCAGTG 2 cut(s) 867, 928
BtsIMutI CAGTG 5 cut(s) 204, 867, 911, 928, 1045
Cac8I GCNNGC 2 cut(s) 452, 645
CfoI GCGC 1 cut(s) 1061
Cfr13I GGNCC 5 cut(s) 808, 842, 937, 957, 958
Cfr9I CCCGGG 1 cut(s) 457
Csp6I GTAC 4 cut(s) 309, 652, 991, 1054
CviQI GTAC 4 cut(s) 309, 652, 991, 1054
DdeI CTNAG 2 cut(s) 702, 849
DpnI GATC 4 cut(s) 397, 471, 520, 689
DpnII GATC 4 cut(s) 395, 469, 518, 687
Eco130I CCWWGG 2 cut(s) 612, 961
Eco24I GRGCYC 2 cut(s) 649, 961
Eco47I GGWCC 1 cut(s) 842
Eco57I CTGAAG 2 cut(s) 887, 965
Eco72I CACGTG 1 cut(s) 1047
Eco88I CYCGRG 2 cut(s) 457, 514
EcoO109I RGGNCCY 1 cut(s) 957
EcoRII CCWGG 1 cut(s) 933
EcoT14I CCWWGG 2 cut(s) 612, 961
EcoT38I GRGCYC 2 cut(s) 649, 961
ErhI CCWWGG 2 cut(s) 612, 961
FaqI GGGAC 1 cut(s) 54
FauI CCCGC 4 cut(s) 443, 636, 948, 1043
FblI GTMKAC 1 cut(s) 1192
Fnu4HI GCNGC 3 cut(s) 448, 694, 710
FokI GGATG 4 cut(s) 156, 277, 358, 965
FriOI GRGCYC 2 cut(s) 649, 961
Fsp4HI GCNGC 3 cut(s) 448, 694, 710
FspBI CTAG 2 cut(s) 605, 1196
GlaI GCGC 1 cut(s) 1060
GluI GCNGC 3 cut(s) 448, 694, 710
GsaI CCCAGC 1 cut(s) 336
HaeII RGCGCY 1 cut(s) 1062
HaeIII GGCC 5 cut(s) 810, 875, 884, 938, 959
HapII CCGG 5 cut(s) 375, 444, 458, 728, 791
HhaI GCGC 1 cut(s) 1061
Hin6I GCGC 1 cut(s) 1059
HinP1I GCGC 1 cut(s) 1059
HincII GTYRAC 1 cut(s) 585
HindII GTYRAC 1 cut(s) 585
HindIII AAGCTT 2 cut(s) 93, 899
HinfI GANTC 4 cut(s) 43, 236, 658, 1118
HpaII CCGG 5 cut(s) 375, 444, 458, 728, 791
HphI GGTGA 3 cut(s) 135, 406, 886
Hpy166II GTNNAC 3 cut(s) 585, 845, 1193
Hpy188I TCNGA 4 cut(s) 42, 663, 983, 1175
Hpy188III TCNNGA 4 cut(s) 467, 516, 759, 1001
Hpy8I GTNNAC 3 cut(s) 585, 845, 1193
Hpy99I CGWCG 1 cut(s) 635
HpyAV CCTTC 1 cut(s) 905
HpyCH4III ACNGT 1 cut(s) 208
HpyCH4IV ACGT 6 cut(s) 16, 563, 675, 753, 1046, 1106
HpyCH4V TGCA 7 cut(s) 55, 101, 393, 483, 568, 747, 860
HpyF10VI GCNNNNNNNGC 6 cut(s) 447, 554, 881, 1031, 1056, 1065
HpyF3I CTNAG 2 cut(s) 702, 849
HpySE526I ACGT 6 cut(s) 16, 563, 675, 753, 1046, 1106
HspAI GCGC 1 cut(s) 1059
Kzo9I GATC 4 cut(s) 395, 469, 518, 687
LmnI GCTCC 4 cut(s) 446, 652, 701, 1092
Lsp1109I GCAGC 2 cut(s) 434, 680
LweI GCATC 3 cut(s) 299, 492, 1142
MaeI CTAG 2 cut(s) 605, 1196
MaeII ACGT 6 cut(s) 16, 563, 675, 753, 1046, 1106
MaeIII GTNAC 2 cut(s) 208, 590
MalI GATC 4 cut(s) 397, 471, 520, 689
MbiI CCGCTC 3 cut(s) 441, 701, 712
MboI GATC 4 cut(s) 395, 469, 518, 687
MboII GAAGA 1 cut(s) 351
MfeI CAATTG 1 cut(s) 1076
MflI RGATCY 2 cut(s) 469, 518
MhlI GDGCHC 4 cut(s) 456, 498, 649, 961
MluCI AATT 6 cut(s) 56, 110, 327, 1076, 1099, 1111
MlyI GAGTC 1 cut(s) 230
MmeI TCCRAC 1 cut(s) 686
MroXI GAANNNNTTC 2 cut(s) 47, 754
MseI TTAA 2 cut(s) 135, 888
MslI CAYNNNNRTG 1 cut(s) 850
MspA1I CMGCKG 2 cut(s) 233, 943
MspI CCGG 5 cut(s) 375, 444, 458, 728, 791
MspR9I CCNGG 4 cut(s) 458, 459, 729, 935
MunI CAATTG 1 cut(s) 1076
MvaI CCWGG 1 cut(s) 935
MwoI GCNNNNNNNGC 6 cut(s) 447, 554, 881, 1031, 1056, 1065
NciI CCSGG 3 cut(s) 458, 459, 729
NcoI CCATGG 1 cut(s) 961
NdeII GATC 4 cut(s) 395, 469, 518, 687
NlaIV GGNNCC 8 cut(s) 455, 610, 648, 826, 843, 939, 958, 959
NmuCI GTSAC 2 cut(s) 208, 590
NspI RCATGY 3 cut(s) 393, 583, 591
OliI CACNNNNGTG 1 cut(s) 850
PciI ACATGT 2 cut(s) 579, 587
PdmI GAANNNNTTC 2 cut(s) 47, 754
PfeI GAWTC 3 cut(s) 43, 658, 1118
PkrI GCNGC 3 cut(s) 449, 695, 711
PleI GAGTC 1 cut(s) 230
PmaCI CACGTG 1 cut(s) 1047
PmlI CACGTG 1 cut(s) 1047
PpsI GAGTC 1 cut(s) 230
Ppu21I YACGTR 1 cut(s) 1047
PscI ACATGT 2 cut(s) 579, 587
Psp1406I AACGTT 1 cut(s) 753
Psp6I CCWGG 1 cut(s) 933
PspCI CACGTG 1 cut(s) 1047
PspFI CCCAGC 1 cut(s) 332
PspGI CCWGG 1 cut(s) 933
PspN4I GGNNCC 8 cut(s) 455, 610, 648, 826, 843, 939, 958, 959
PspOMI GGGCCC 1 cut(s) 957
PspPI GGNCC 5 cut(s) 808, 842, 937, 957, 958
PstI CTGCAG 1 cut(s) 103
PsuI RGATCY 2 cut(s) 469, 518
PvuII CAGCTG 1 cut(s) 233
RsaI GTAC 4 cut(s) 310, 653, 992, 1055
RsaNI GTAC 4 cut(s) 309, 652, 991, 1054
RseI CAYNNNNRTG 1 cut(s) 850
SaqAI TTAA 2 cut(s) 135, 888
SatI GCNGC 3 cut(s) 448, 694, 710
Sau3AI GATC 4 cut(s) 395, 469, 518, 687
Sau96I GGNCC 5 cut(s) 808, 842, 937, 957, 958
ScaI AGTACT 2 cut(s) 310, 992
SchI GAGTC 1 cut(s) 230
ScrFI CCNGG 4 cut(s) 458, 459, 729, 935
SduI GDGCHC 4 cut(s) 456, 498, 649, 961
SfaNI GCATC 3 cut(s) 299, 492, 1142
SfcI CTRYAG 2 cut(s) 99, 304
SfiI GGCCNNNNNGGCC 1 cut(s) 881
SinI GGWCC 1 cut(s) 842
SmaI CCCGGG 1 cut(s) 459
SmiMI CAYNNNNRTG 1 cut(s) 850
Sse9I AATT 6 cut(s) 56, 110, 327, 1076, 1099, 1111
SsiI CCGC 9 cut(s) 405, 439, 450, 643, 666, 699, 710, 941, 1050
SspMI CTAG 2 cut(s) 605, 1196
StyD4I CCNGG 4 cut(s) 456, 457, 727, 933
StyI CCWWGG 2 cut(s) 612, 961
TaaI ACNGT 1 cut(s) 208
TaiI ACGT 6 cut(s) 19, 566, 678, 756, 1049, 1109
TaqI TCGA 2 cut(s) 466, 633
TasI AATT 6 cut(s) 56, 110, 327, 1076, 1099, 1111
TatI WGTACW 2 cut(s) 308, 990
TauI GCSGC 1 cut(s) 712
TfiI GAWTC 3 cut(s) 43, 658, 1118
Tru1I TTAA 2 cut(s) 135, 888
Tru9I TTAA 2 cut(s) 135, 888
TscAI CASTG 5 cut(s) 211, 867, 911, 928, 1045
TseFI GTSAC 2 cut(s) 208, 590
TseI GCWGC 2 cut(s) 447, 693
Tsp45I GTSAC 2 cut(s) 208, 590
TspDTI ATGAA 3 cut(s) 585, 670, 671
TspGWI ACGGA 2 cut(s) 619, 639
TspMI CCCGGG 1 cut(s) 457
TspRI CASTG 5 cut(s) 211, 867, 911, 928, 1045
VpaK11BI GGWCC 1 cut(s) 842
XceI RCATGY 3 cut(s) 393, 583, 591
XmaI CCCGGG 1 cut(s) 457
XmiI GTMKAC 1 cut(s) 1192
XmnI GAANNNNTTC 2 cut(s) 47, 754
XspI CTAG 2 cut(s) 605, 1196
ZrmI AGTACT 2 cut(s) 310, 992
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.