Rorug07G0009000

Wall-associated receptor kinase galacturonan-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0013565
Physical Location & Seq
Forward (+)
736905 .. 746517
9613 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0009000.1

Sequence Viewer

Length: 2412 bp
ATGCAATCCAATCCGTTGGGCTTCACTTTTTATTTTGTTTTCTCTAATCCTTCCAACCAGGAATATGAGGGCCAAATTCATGAAGCATGTGAAATTATATTTCAAAGCATATCAGTATTAGTTGGACCAAACCCATTTTGTCGCGCAAGCAAATCAACTTTCTTGCCTGATACGATGGGAAAGGAAATCAGTGCTCAGCGGAAACATATAAACCTATATCATCTAATGGGAAGGGAAATCCCAGGAGATATGCTCAATGTTGAGCGTGCTGTAATACTTTACAACCTATGTTGGTTGTCTTTGAAGGACCGTAAGAAGGATACCAGCAATAATCCCCATGTGCTGATTGATATTCCTATATCAAAACTAGTGTCTTGGTTGATGCTTTTATTCGTCCTCTGCCGTGAGGTTCCTGCACTTTTTCAGAAGGTTTCGAGATTGCTGGCTGCTATTTTCTCACTGTCTACCTCAAGTGTGCTTGAAGCTCCATTGTCAACTTCTTGCAAAGATCTCTGTGGACGTCATTGGGTTTGTTTTTTTCATCAAGCTTCACTTGGAATTCAGTTTAATTACCAGTTTTCCATGAAAGAAGTCCAGCAGGGTACAGACACTAAGGGTTCACACATGGCATCAGGAGCACAGAGTTTACCTAGGCTTGCACCAGAATCTATTCTAAACCTTAAAGACTTTGTAAAAGAATTTTTTGAAGGCCTTCCTGGCACCACAGTCATCTGTCTAAGTTTACTTGGCTGCCCATATACTAGTATGTTTCAGGAATTATTGTCTTGTTCATCTGCTCCTGCATGGATTATGGTGTCTCGCTTAAACCCTAAGAATCTTCCTATACATATTCTTCTGCCGGTGCATTCAGTACCAGGAGATGGTGTTACAAATTCTGGTGGGAATGATGGTGGTCAGTGGCGCTGTCCATGGGGTTCCACTGTGGTTGATGATGTAGCTCCGAATTTTAGAATGCTATTGGAGGAGAATTTCAATTTATCAAATTACGAGTTAGCTTCAAGTCCTGCTGAAAAGAAAGATCATATATATAATGTGTGGGAAGCGAGACGGAGGATTGATGATCGCCTTTGTACATTGCTGAAAAACTTGGAAGATTCATGGTTTGGTAGTACTTTGAAATATGTGCTTCTTGGTGAAGTGTCCAACTGCAAGGATCTGGACACGTTATGTGAGAACCTTGTGAGGAAATTGAAATCTCAATTCACGGACATAGATTCTAGTCTTCTGAAAGTCATCGTCCTAGGGGTGTCCAAATATGGATTTAAAGGAGGCACAGAACAAGTTATGTTGGAGTTTTGCCTTAAGAAAGGTTGTTTCTTTAGTGATGTTCATTTCAAGGATGACCATTGTGCTGATGAATTGCGTGGCTTTGCTTCCAAACTACATGAAGCAGTGAAGAAGGTCAAAGGCTTTAATAGAATTATAAAGCCGACAATCTTAGTCCCAGACTTTGACGTCCAGATGCTTCCTTGGAATTTACCGGTACTAAAGAACCAGGAGGTTTATCGCATGCCATCAGTTTGGAGTATATTGGCAGCATATAGAAATGAGCGCAAGATCAATCCTTTGGATGCCTATTATTTGTTCAACCCTGATGATAGCCTGAATTGCAAACCCGATTTTGACAAATTGTTTAGAGATAATAAGTTGAAGGGGAAGGTTGGACATGCACCCCCAACTGAAGAACTGGCAGCAGCTTTGGAAAGCAGGGATCTCTTCATATATATAGGCCATGGAGATGGGTCGTTATATATTCCTAATGATAGGATCGAGAGACTCCGAAACTGTGCTGGGGCTTTCCTAATGGGATGCAGTAGTGCTTCTTTGAAACTAGATGGGTGTTATTTCCCACGAGTTACCACAGGAGCTTCTGTTGTGGAAAAGGATGATGTTTTGAAGGGGGGTTTTGGTGGGAGTAAAGCGTATGGTGATTTATCAAAATGTGGGAAGCCTGCTATGACTGAGGAGTCATCACAAAAGCAACAGCCTTCTGACATTGATGAAAGTAATATCAAAAGGAAAGGAGGGGTTGTTACAGAGGAAGATATAGCAGATGAAGATTCTGATTATGATTATGATTTTTTTGATGAACGTGTCGTGAAGAGGTCTGATCCAACTAAGGAAGAAATCTTGGAGATTATGGCGTACGAGCACGATCATAAGAGATGTATCGAATTACAGGAAAAGGCTTTGAAGGGGGAGTATGCATCTCAGAATCCAGCTGATTCTCCACTTTTCAATTGGCCCTGGGAGGAGGAATTTGAGAGCAAGCCGATGGATGTATCAGAGTTGGTGGAAATATTAGATTCATTGGAAGTATTAGGAGAAGTTGATGGAACATATGAGTGTCTAGATGCAGCCTTCAGGGAGGGGGTTGGCTTTTTTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind

Protein Analysis

803

Amino Acids

90.39

Weight (kDa)

5.26

Isoelectric Point (pI)

38.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Separin_C PF03568 268 - 624 2.8e-51 Separin, protease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013565)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50290
fragaria_vesca FvH4_5g05500
malus_domestica MD06G1172100.v1.1 MD14G1178000.v1.1
prunus_persica Prupe.5G171500_v2.0.a1
pyrus_communis pycom06g15330 pycom14g14850
rosa_chinensis RchiOBHm_Chr7g0193341
rosa_laevigata RLG00000004278
rosa_multiflora Rmu_ssc0000092.1_g000009
rosa_roxburghii Rroxscaffold_3G00262200
rosa_rugosa Rorug07G0009000
rosa_samantha Rh7AG134800 Rh7AG135200 Rh7BG135500 Rh7CG138900 Rh7DG136900
rosa_wichuraiana Rw7G011520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1445
AasI GACNNNNNNGTC 2 cut(s) 1475, 1987
AatII GACGTC 2 cut(s) 523, 1479
AccB1I GGYRCC 1 cut(s) 719
AccB7I CCANNNNNTGG 1 cut(s) 881
AccI GTMKAC 1 cut(s) 464
AccII CGCG 1 cut(s) 144
AciI CCGC 1 cut(s) 199
AclWI GGATC 4 cut(s) 1182, 1740, 1796, 2126
AcsI RAATTY 8 cut(s) 75, 558, 698, 892, 964, 988, 1495, 2279
AcuI CTGAAG 2 cut(s) 1722, 2368
AcyI GRCGYC 2 cut(s) 520, 1476
AfaI GTAC 6 cut(s) 604, 873, 1093, 1132, 1506, 2168
AfiI CCNNNNNNNGG 2 cut(s) 316, 881
AflII CTTAAG 1 cut(s) 1322
AflIII ACRYGT 2 cut(s) 1182, 2113
AgeI ACCGGT 1 cut(s) 1501
AhlI ACTAGT 2 cut(s) 367, 761
AjnI CCWGG 6 cut(s) 57, 241, 715, 874, 1515, 2267
AluBI AGCT 7 cut(s) 485, 548, 959, 1016, 1718, 1889, 2243
AluI AGCT 7 cut(s) 485, 548, 959, 1016, 1718, 1889, 2243
Alw21I GWGCWC 3 cut(s) 196, 640, 2175
Alw26I GTCTC 3 cut(s) 822, 1060, 1789
AlwI GGATC 4 cut(s) 1182, 1740, 1796, 2126
AoxI GGCC 4 cut(s) 70, 709, 1750, 2264
ApeKI GCWGC 6 cut(s) 446, 750, 1556, 1712, 1715, 2378
ApoI RAATTY 8 cut(s) 75, 558, 698, 892, 964, 988, 1495, 2279
ArsI GACNNNNNNTTYG 2 cut(s) 356, 388
AsiGI ACCGGT 1 cut(s) 1501
Asp700I GAANNNNTTC 2 cut(s) 669, 711
AspA2I CCTAGG 2 cut(s) 650, 1261
AspLEI GCGC 3 cut(s) 146, 924, 1575
AspS9I GGNCC 4 cut(s) 70, 125, 307, 2265
AsuHPI GGTGA 2 cut(s) 1166, 1961
AvaII GGWCC 2 cut(s) 125, 307
AvrII CCTAGG 2 cut(s) 650, 1261
BanI GGYRCC 1 cut(s) 719
BauI CACGAG 1 cut(s) 1872
BbsI GAAGAC 1 cut(s) 1235
Bbv12I GWGCWC 3 cut(s) 196, 640, 2175
BbvI GCAGC 6 cut(s) 433, 737, 1568, 1724, 1727, 2390
BccI CCATC 8 cut(s) 169, 875, 902, 1543, 1754, 1850, 2290, 2348
BceAI ACGGC 1 cut(s) 387
BciT130I CCWGG 6 cut(s) 59, 243, 717, 876, 1517, 2269
BciVI GTATCC 1 cut(s) 313
BcoDI GTCTC 3 cut(s) 822, 1060, 1789
BcuI ACTAGT 2 cut(s) 367, 761
BfaI CTAG 7 cut(s) 368, 651, 762, 1239, 1262, 1853, 2372
BfoI RGCGCY 1 cut(s) 925
BfrI CTTAAG 1 cut(s) 1322
BfuI GTATCC 1 cut(s) 313
BglI GCCNNNNNGGC 1 cut(s) 717
BglII AGATCT 1 cut(s) 508
BisI GCNGC 6 cut(s) 447, 751, 1557, 1713, 1716, 2379
BlnI CCTAGG 2 cut(s) 650, 1261
BlpI GCTNAGC 1 cut(s) 195
BlsI GCNGC 6 cut(s) 448, 752, 1558, 1714, 1717, 2380
BmcAI AGTACT 1 cut(s) 1132
Bme1390I CCNGG 6 cut(s) 59, 243, 717, 876, 1517, 2269
Bme18I GGWCC 2 cut(s) 125, 307
BmgT120I GGNCC 4 cut(s) 70, 125, 307, 2265
BmiI GGNNCC 3 cut(s) 411, 721, 937
BmrFI CCNGG 6 cut(s) 59, 243, 717, 876, 1517, 2269
BmsI GCATC 7 cut(s) 372, 638, 1473, 1582, 1820, 2237, 2365
BpiI GAAGAC 1 cut(s) 1235
BplI GAGNNNNNCTC 2 cut(s) 237, 269
Bpu1102I GCTNAGC 1 cut(s) 195
BpuEI CTTGAG 1 cut(s) 454
BsaHI GRCGYC 2 cut(s) 520, 1476
BsaJI CCNNGG 8 cut(s) 241, 650, 929, 1261, 1490, 1753, 2267, 2268
BsaWI WCCGGW 1 cut(s) 1501
BsaXI ACNNNNNCTCC 6 cut(s) 870, 900, 1878, 1908, 2037, 2067
Bsc4I CCNNNNNNNGG 2 cut(s) 316, 881
Bse118I RCCGGY 2 cut(s) 859, 1501
Bse1I ACTGG 2 cut(s) 574, 1713
Bse3DI GCAATG 1 cut(s) 1094
BseBI CCWGG 6 cut(s) 59, 243, 717, 876, 1517, 2269
BseDI CCNNGG 8 cut(s) 241, 650, 929, 1261, 1490, 1753, 2267, 2268
BseGI GGATG 5 cut(s) 1366, 1597, 1835, 1912, 2305
BseLI CCNNNNNNNGG 2 cut(s) 316, 881
BseMI GCAATG 1 cut(s) 1094
BseMII CTCAG 3 cut(s) 209, 1974, 2246
BseNI ACTGG 2 cut(s) 574, 1713
BseRI GAGGAG 3 cut(s) 998, 2000, 2288
BseXI GCAGC 6 cut(s) 433, 737, 1568, 1724, 1727, 2390
BseYI CCCAGC 1 cut(s) 1811
BsgI GTGCAG 1 cut(s) 399
Bsh1236I CGCG 1 cut(s) 144
BshFI GGCC 4 cut(s) 72, 711, 1752, 2266
BshNI GGYRCC 1 cut(s) 719
BshTI ACCGGT 1 cut(s) 1501
BsiHKAI GWGCWC 3 cut(s) 196, 640, 2175
BsiSI CCGG 2 cut(s) 860, 1502
BsiWI CGTACG 1 cut(s) 2166
BslFI GGGAC 1 cut(s) 1448
BslI CCNNNNNNNGG 2 cut(s) 316, 881
BsmAI GTCTC 3 cut(s) 822, 1060, 1789
BsmBI CGTCTC 1 cut(s) 1060
BsmFI GGGAC 1 cut(s) 1448
BsmI GAATGC 2 cut(s) 865, 978
BsnI GGCC 4 cut(s) 72, 711, 1752, 2266
Bsp1286I GDGCHC 3 cut(s) 196, 640, 2175
Bsp1407I TGTACA 1 cut(s) 1091
Bsp143I GATC 9 cut(s) 508, 1039, 1081, 1174, 1578, 1732, 1788, 2131, 2176
Bsp1720I GCTNAGC 1 cut(s) 195
Bsp19I CCATGG 2 cut(s) 929, 1753
BspACI CCGC 1 cut(s) 199
BspANI GGCC 4 cut(s) 72, 711, 1752, 2266
BspCNI CTCAG 3 cut(s) 208, 1975, 2245
BspFNI CGCG 1 cut(s) 144
BspHI TCATGA 1 cut(s) 79
BspLI GGNNCC 3 cut(s) 411, 721, 937
BspPI GGATC 4 cut(s) 1182, 1740, 1796, 2126
BspT107I GGYRCC 1 cut(s) 719
BspTI CTTAAG 1 cut(s) 1322
BsrDI GCAATG 1 cut(s) 1094
BsrFI RCCGGY 2 cut(s) 859, 1501
BsrGI TGTACA 1 cut(s) 1091
BsrI ACTGG 2 cut(s) 574, 1713
BssAI RCCGGY 2 cut(s) 859, 1501
BssECI CCNNGG 8 cut(s) 241, 650, 929, 1261, 1490, 1753, 2267, 2268
BssMI GATC 9 cut(s) 508, 1039, 1081, 1174, 1578, 1732, 1788, 2131, 2176
BssNI GRCGYC 2 cut(s) 520, 1476
BssSI CACGAG 1 cut(s) 1872
BssT1I CCWWGG 5 cut(s) 650, 929, 1261, 1490, 1753
Bst2BI CACGAG 1 cut(s) 1872
Bst2UI CCWGG 6 cut(s) 59, 243, 717, 876, 1517, 2269
Bst4CI ACNGT 5 cut(s) 311, 462, 727, 943, 1808
Bst6I CTCTTC 2 cut(s) 1742, 2117
BstACI GRCGYC 2 cut(s) 520, 1476
BstAFI CTTAAG 1 cut(s) 1322
BstAUI TGTACA 1 cut(s) 1091
BstC8I GCNNGC 7 cut(s) 148, 267, 444, 657, 1532, 1974, 2291
BstDEI CTNAG 8 cut(s) 195, 612, 737, 831, 1459, 1983, 2139, 2232
BstDSI CCRYGG 2 cut(s) 929, 1753
BstF5I GGATG 5 cut(s) 1366, 1597, 1835, 1912, 2305
BstFNI CGCG 1 cut(s) 144
BstH2I RGCGCY 1 cut(s) 925
BstHHI GCGC 3 cut(s) 146, 924, 1575
BstKTI GATC 9 cut(s) 511, 1042, 1084, 1177, 1581, 1735, 1791, 2134, 2179
BstMAI GTCTC 3 cut(s) 822, 1060, 1789
BstMBI GATC 9 cut(s) 508, 1039, 1081, 1174, 1578, 1732, 1788, 2131, 2176
BstMWI GCNNNNNNNGC 3 cut(s) 635, 717, 1629
BstNI CCWGG 6 cut(s) 59, 243, 717, 876, 1517, 2269
BstNSI RCATGY 3 cut(s) 90, 1534, 1691
BstSCI CCNGG 6 cut(s) 57, 241, 715, 874, 1515, 2267
BstUI CGCG 1 cut(s) 144
BstV1I GCAGC 6 cut(s) 433, 737, 1568, 1724, 1727, 2390
BstV2I GAAGAC 1 cut(s) 1235
BstX2I RGATCY 3 cut(s) 508, 1174, 1732
BstXI CCANNNNNNTGG 3 cut(s) 16, 1542, 1760
BstYI RGATCY 3 cut(s) 508, 1174, 1732
BsuI GTATCC 1 cut(s) 313
BsuRI GGCC 4 cut(s) 72, 711, 1752, 2266
BtgI CCRYGG 2 cut(s) 929, 1753
BtsCI GGATG 5 cut(s) 1366, 1597, 1835, 1912, 2305
BtsI GCAGTG 1 cut(s) 1419
BtsIMutI CAGTG 5 cut(s) 196, 458, 923, 939, 1419
Cac8I GCNNGC 7 cut(s) 148, 267, 444, 657, 1532, 1974, 2291
CciI TCATGA 1 cut(s) 79
CfoI GCGC 3 cut(s) 146, 924, 1575
Cfr10I RCCGGY 2 cut(s) 859, 1501
Cfr13I GGNCC 4 cut(s) 70, 125, 307, 2265
Csp6I GTAC 6 cut(s) 603, 872, 1092, 1131, 1505, 2167
CspAI ACCGGT 1 cut(s) 1501
CspCI CAANNNNNGTGG 2 cut(s) 928, 963
CviQI GTAC 6 cut(s) 603, 872, 1092, 1131, 1505, 2167
DdeI CTNAG 8 cut(s) 195, 612, 737, 831, 1459, 1983, 2139, 2232
DpnI GATC 9 cut(s) 510, 1041, 1083, 1176, 1580, 1734, 1790, 2133, 2178
DpnII GATC 9 cut(s) 508, 1039, 1081, 1174, 1578, 1732, 1788, 2131, 2176
DraI TTTAAA 1 cut(s) 1285
DrdI GACNNNNNNGTC 2 cut(s) 1475, 1987
DseDI GACNNNNNNGTC 2 cut(s) 1475, 1987
Eam1104I CTCTTC 2 cut(s) 1742, 2117
EarI CTCTTC 2 cut(s) 1742, 2117
Eco130I CCWWGG 5 cut(s) 650, 929, 1261, 1490, 1753
Eco147I AGGCCT 1 cut(s) 711
Eco47I GGWCC 2 cut(s) 125, 307
Eco57I CTGAAG 2 cut(s) 1722, 2368
EcoRI GAATTC 1 cut(s) 558
EcoRII CCWGG 6 cut(s) 57, 241, 715, 874, 1515, 2267
EcoT14I CCWWGG 5 cut(s) 650, 929, 1261, 1490, 1753
EcoT22I ATGCAT 1 cut(s) 2230
ErhI CCWWGG 5 cut(s) 650, 929, 1261, 1490, 1753
Esp3I CGTCTC 1 cut(s) 1060
FalI AAGNNNNNCTT 2 cut(s) 537, 569
FaqI GGGAC 1 cut(s) 1448
FauNDI CATATG 1 cut(s) 2362
FblI GTMKAC 1 cut(s) 464
Fnu4HI GCNGC 6 cut(s) 447, 751, 1557, 1713, 1716, 2379
FokI GGATG 5 cut(s) 1373, 1604, 1842, 1919, 2312
Fsp4HI GCNGC 6 cut(s) 447, 751, 1557, 1713, 1716, 2379
FspBI CTAG 7 cut(s) 368, 651, 762, 1239, 1262, 1853, 2372
GlaI GCGC 3 cut(s) 145, 923, 1574
GluI GCNGC 6 cut(s) 447, 751, 1557, 1713, 1716, 2379
GsaI CCCAGC 1 cut(s) 1815
HaeII RGCGCY 1 cut(s) 925
HaeIII GGCC 4 cut(s) 72, 711, 1752, 2266
HapII CCGG 2 cut(s) 860, 1502
HhaI GCGC 3 cut(s) 146, 924, 1575
Hin1I GRCGYC 2 cut(s) 520, 1476
Hin6I GCGC 3 cut(s) 144, 922, 1573
HinP1I GCGC 3 cut(s) 144, 922, 1573
HincII GTYRAC 1 cut(s) 495
HindII GTYRAC 1 cut(s) 495
HindIII AAGCTT 1 cut(s) 546
HpaII CCGG 2 cut(s) 860, 1502
HphI GGTGA 2 cut(s) 1166, 1961
Hpy166II GTNNAC 6 cut(s) 465, 495, 518, 620, 647, 743
Hpy188I TCNGA 9 cut(s) 426, 963, 1248, 1802, 2014, 2086, 2131, 2235, 2308
Hpy188III TCNNGA 9 cut(s) 80, 435, 633, 773, 1178, 1480, 1792, 2119, 2372
Hpy8I GTNNAC 6 cut(s) 465, 495, 518, 620, 647, 743
HpyCH4III ACNGT 5 cut(s) 311, 462, 727, 943, 1808
HpyCH4IV ACGT 4 cut(s) 520, 1184, 1476, 2113
HpyF10VI GCNNNNNNNGC 3 cut(s) 635, 717, 1629
HpyF3I CTNAG 8 cut(s) 195, 612, 737, 831, 1459, 1983, 2139, 2232
HpySE526I ACGT 4 cut(s) 520, 1184, 1476, 2113
Hsp92I GRCGYC 2 cut(s) 520, 1476
HspAI GCGC 3 cut(s) 144, 922, 1573
Kzo9I GATC 9 cut(s) 508, 1039, 1081, 1174, 1578, 1732, 1788, 2131, 2176
LmnI GCTCC 5 cut(s) 490, 635, 802, 964, 1886
Lsp1109I GCAGC 6 cut(s) 433, 737, 1568, 1724, 1727, 2390
LweI GCATC 7 cut(s) 372, 638, 1473, 1582, 1820, 2237, 2365
MaeI CTAG 7 cut(s) 368, 651, 762, 1239, 1262, 1853, 2372
MaeII ACGT 4 cut(s) 520, 1184, 1476, 2113
MaeIII GTNAC 3 cut(s) 886, 1876, 2053
MalI GATC 9 cut(s) 510, 1041, 1083, 1176, 1580, 1734, 1790, 2133, 2178
MboI GATC 9 cut(s) 508, 1039, 1081, 1174, 1578, 1732, 1788, 2131, 2176
MfeI CAATTG 1 cut(s) 2260
MflI RGATCY 3 cut(s) 508, 1174, 1732
MhlI GDGCHC 3 cut(s) 196, 640, 2175
MlyI GAGTC 2 cut(s) 1791, 1997
MmeI TCCRAC 6 cut(s) 78, 103, 1188, 1290, 1663, 2159
Mph1103I ATGCAT 1 cut(s) 2230
MroXI GAANNNNTTC 2 cut(s) 669, 711
MseI TTAA 7 cut(s) 567, 681, 824, 1284, 1323, 1434, 2406
MslI CAYNNNNRTG 2 cut(s) 1758, 2365
MspA1I CMGCKG 2 cut(s) 199, 2243
MspCI CTTAAG 1 cut(s) 1322
MspI CCGG 2 cut(s) 860, 1502
MspR9I CCNGG 6 cut(s) 59, 243, 717, 876, 1517, 2269
MunI CAATTG 1 cut(s) 2260
Mva1269I GAATGC 2 cut(s) 865, 978
MvaI CCWGG 6 cut(s) 59, 243, 717, 876, 1517, 2269
MvnI CGCG 1 cut(s) 144
MwoI GCNNNNNNNGC 3 cut(s) 635, 717, 1629
NcoI CCATGG 2 cut(s) 929, 1753
NdeI CATATG 1 cut(s) 2362
NdeII GATC 9 cut(s) 508, 1039, 1081, 1174, 1578, 1732, 1788, 2131, 2176
NlaIV GGNNCC 3 cut(s) 411, 721, 937
NsiI ATGCAT 1 cut(s) 2230
NspI RCATGY 3 cut(s) 90, 1534, 1691
PaeI GCATGC 1 cut(s) 1534
PagI TCATGA 1 cut(s) 79
PasI CCCWGGG 1 cut(s) 2268
PceI AGGCCT 1 cut(s) 711
PctI GAATGC 2 cut(s) 865, 978
PdmI GAANNNNTTC 2 cut(s) 669, 711
PfeI GAWTC 8 cut(s) 665, 835, 1115, 1235, 2081, 2236, 2246, 2327
Pfl23II CGTACG 1 cut(s) 2166
PflMI CCANNNNNTGG 1 cut(s) 881
PinAI ACCGGT 1 cut(s) 1501
PkrI GCNGC 6 cut(s) 448, 752, 1558, 1714, 1717, 2380
PleI GAGTC 2 cut(s) 1791, 1996
PpsI GAGTC 2 cut(s) 1791, 1996
PsiI TTATAA 1 cut(s) 1445
Psp6I CCWGG 6 cut(s) 57, 241, 715, 874, 1515, 2267
PspFI CCCAGC 1 cut(s) 1811
PspGI CCWGG 6 cut(s) 57, 241, 715, 874, 1515, 2267
PspLI CGTACG 1 cut(s) 2166
PspN4I GGNNCC 3 cut(s) 411, 721, 937
PspPI GGNCC 4 cut(s) 70, 125, 307, 2265
PsuI RGATCY 3 cut(s) 508, 1174, 1732
PvuII CAGCTG 1 cut(s) 2243
RsaI GTAC 6 cut(s) 604, 873, 1093, 1132, 1506, 2168
RsaNI GTAC 6 cut(s) 603, 872, 1092, 1131, 1505, 2167
RseI CAYNNNNRTG 2 cut(s) 1758, 2365
SaqAI TTAA 7 cut(s) 567, 681, 824, 1284, 1323, 1434, 2406
SatI GCNGC 6 cut(s) 447, 751, 1557, 1713, 1716, 2379
Sau3AI GATC 9 cut(s) 508, 1039, 1081, 1174, 1578, 1732, 1788, 2131, 2176
Sau96I GGNCC 4 cut(s) 70, 125, 307, 2265
ScaI AGTACT 1 cut(s) 1132
SchI GAGTC 2 cut(s) 1791, 1997
ScrFI CCNGG 6 cut(s) 59, 243, 717, 876, 1517, 2269
SduI GDGCHC 3 cut(s) 196, 640, 2175
SfaNI GCATC 7 cut(s) 372, 638, 1473, 1582, 1820, 2237, 2365
SinI GGWCC 2 cut(s) 125, 307
SmiMI CAYNNNNRTG 2 cut(s) 1758, 2365
SmlI CTYRAG 2 cut(s) 469, 1322
SmoI CTYRAG 2 cut(s) 469, 1322
SpeI ACTAGT 2 cut(s) 367, 761
SphI GCATGC 1 cut(s) 1534
SseBI AGGCCT 1 cut(s) 711
SsiI CCGC 1 cut(s) 199
SspI AATATT 1 cut(s) 2322
SspMI CTAG 7 cut(s) 368, 651, 762, 1239, 1262, 1853, 2372
StuI AGGCCT 1 cut(s) 711
StyD4I CCNGG 6 cut(s) 57, 241, 715, 874, 1515, 2267
StyI CCWWGG 5 cut(s) 650, 929, 1261, 1490, 1753
TaaI ACNGT 5 cut(s) 311, 462, 727, 943, 1808
TaiI ACGT 4 cut(s) 523, 1187, 1479, 2116
TaqI TCGA 3 cut(s) 434, 1791, 2193
TatI WGTACW 2 cut(s) 1091, 1130
TfiI GAWTC 8 cut(s) 665, 835, 1115, 1235, 2081, 2236, 2246, 2327
Tru1I TTAA 7 cut(s) 567, 681, 824, 1284, 1323, 1434, 2406
Tru9I TTAA 7 cut(s) 567, 681, 824, 1284, 1323, 1434, 2406
TscAI CASTG 5 cut(s) 196, 465, 923, 946, 1419
TseI GCWGC 6 cut(s) 446, 750, 1556, 1712, 1715, 2378
TspGWI ACGGA 2 cut(s) 1084, 1241
TspRI CASTG 5 cut(s) 196, 465, 923, 946, 1419
Van91I CCANNNNNTGG 1 cut(s) 881
Vha464I CTTAAG 1 cut(s) 1322
VpaK11BI GGWCC 2 cut(s) 125, 307
XapI RAATTY 8 cut(s) 75, 558, 698, 892, 964, 988, 1495, 2279
XbaI TCTAGA 1 cut(s) 2371
XceI RCATGY 3 cut(s) 90, 1534, 1691
XcmI CCANNNNNNNNNTGG 1 cut(s) 2259
XmaJI CCTAGG 2 cut(s) 650, 1261
XmiI GTMKAC 1 cut(s) 464
XmnI GAANNNNTTC 2 cut(s) 669, 711
XspI CTAG 7 cut(s) 368, 651, 762, 1239, 1262, 1853, 2372
ZraI GACGTC 2 cut(s) 521, 1477
ZrmI AGTACT 1 cut(s) 1132
Zsp2I ATGCAT 1 cut(s) 2230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.