AT5G50960

Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. Functions as Fe-S scaffold, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Essential for embryo development

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012080
Physical Location & Seq
Forward (+)
20734136 .. 20736138
2003 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G50960.1

Sequence Viewer

Length: 1053 bp
ATGGAGAACGGAGACATTCCAGAGGACGCTAACGAGCATTGCCCAGGTCCTCAATCAGAATCTGCTGGAAAATCAGATTCTTGTGCTGGCTGCCCTAATCAGGAAGCTTGTGCTACTGCTCCTAAAGGACCTGACCCAGATTTGGTTGCCATAGCAGAGAGGATGAGCACTGTTAAGCACAAGATTCTGGTTTTGTCTGGCAAAGGTGGAGTTGGTAAGAGTACTTTCTCAGCTCAGCTCTCGTTTGCCCTTGCGGGCATGGATCATCAAGTAGGTCTGATGGACATTGATATATGCGGTCCAAGCATCCCAAAAATGTTAGGACTTGAAGGGCAAGAGATTCACCAGAGTAACCTTGGATGGTCTCCGGTTTATGTGGAAGACAACCTTGGTGTCATGTCCATAGGTTTCATGCTCCCTAACTCTGATGAAGCTGTGATCTGGAGAGGGCCACGCAAGAATGGTCTCATCAAGCAGTTCTTAAAAGACGTCTACTGGGGAGAGATTGATTACCTTGTTGTTGATGCCCCACCAGGAACCTCAGATGAACACATCTCCATCGTCCAGTACCTTCTACCCACGGGGATCGACGGTGCCATCATTGTCACGACACCGCAGGAAGTCTCCTTGATTGATGTCAGAAAAGAAGTGAGCTTTTGCAAGAAAGTTGGAGTCCCGGTGCTAGGAGTAGTAGAGAACATGAGTGGTTTATCTCAACCGTTGAAGGATGTCAAGTTCATGAAGCTGGCGACAGAGACCGGCTCCTCCATTAACGTGACTGAAGACGTAATCGCTTGCTTAAGAAAAAACGCACCAGAGCTTCTCGACATCGTGGCTTGTAGCGAAGTGTTTGACAGCAGCGGAGGCGGTGCAGAAAGAATGTGTCGGGAAATGGGAGTACCGTTTCTTGGGAAAGTTCCTATGGATCCACAACTTTGCAAAGCAGCTGAACAAGGTAAGTCATGCTTTGAGGATAACAAGTGTTTAATAAGCGCACCTGCGCTTAAAAGCATCATACAGAAAGTCGTTCCTTCAACAGTGATGACCGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0042802 GO:0042803 GO:0044424 GO:0044444 GO:0044464 GO:0046983 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ParA

Protein Analysis

350

Amino Acids

37.31

Weight (kDa)

4.79

Isoelectric Point (pI)

44.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ParA PF10609 58 - 243 3.5e-80 NUBPL iron-transfer P-loop NTPase
ArsA_ATPase PF02374 60 - 135 3.3e-06 Anion-transporting ATPase
CbiA PF01656 62 - 320 8.2e-12 CobQ/CobB/MinD/ParA nucleotide binding domain
ParA PF10609 280 - 342 5.6e-07 NUBPL iron-transfer P-loop NTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50960
fragaria_vesca FvH4_6g52730
malus_domestica MD09G1017000.v1.1 MD17G1017500.v1.1
prunus_persica Prupe.3G300400_v2.0.a1 Prupe.3G300400_v2.0.a1
pyrus_communis pycom111g01380 pycom17g01410
rosa_chinensis RchiOBHm_Chr2g0174341
rosa_laevigata RLG00000022259
rosa_multiflora Rmu_sc0003649.1_g000032 Rmu_sc0007150.1_g000002
rosa_roxburghii Rroxscaffold_2G00078280
rosa_rugosa Rorug02G0576400
rosa_samantha Rh2AG657300 Rh2BG668300 Rh2CG631400 Rh2DG681900
rosa_wichuraiana Rw2G053830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1006
AatII GACGTC 1 cut(s) 492
Acc36I ACCTGC 1 cut(s) 1006
AccB1I GGYRCC 1 cut(s) 593
AccI GTMKAC 1 cut(s) 492
AciI CCGC 5 cut(s) 254, 297, 614, 861, 867
AclWI GGATC 4 cut(s) 270, 593, 920, 933
AcuI CTGAAG 1 cut(s) 801
AcyI GRCGYC 1 cut(s) 489
AfaI GTAC 3 cut(s) 223, 569, 900
AfiI CCNNNNNNNGG 4 cut(s) 100, 142, 683, 908
AflII CTTAAG 1 cut(s) 799
AgsI TTSAA 3 cut(s) 329, 724, 1035
AjnI CCWGG 2 cut(s) 43, 532
AjuI GAANNNNNNNTTGG 2 cut(s) 372, 404
AloI GAACNNNNNNTCC 2 cut(s) 719, 751
AluBI AGCT 8 cut(s) 107, 233, 238, 434, 654, 745, 820, 947
AluI AGCT 8 cut(s) 107, 233, 238, 434, 654, 745, 820, 947
Alw21I GWGCWC 1 cut(s) 170
Alw26I GTCTC 5 cut(s) 6, 369, 470, 628, 749
AlwI GGATC 4 cut(s) 270, 593, 920, 933
AlwNI CAGNNNCTG 1 cut(s) 62
AoxI GGCC 1 cut(s) 449
ApeKI GCWGC 3 cut(s) 90, 858, 944
AspLEI GCGC 2 cut(s) 995, 1003
AspS9I GGNCC 4 cut(s) 47, 128, 299, 449
AsuC2I CCSGG 1 cut(s) 677
AsuHPI GGTGA 1 cut(s) 335
AvaII GGWCC 3 cut(s) 47, 128, 299
BamHI GGATCC 1 cut(s) 925
BanI GGYRCC 1 cut(s) 593
BbsI GAAGAC 2 cut(s) 387, 789
Bbv12I GWGCWC 1 cut(s) 170
BbvI GCAGC 3 cut(s) 77, 870, 956
BccI CCATC 4 cut(s) 274, 354, 566, 605
BciT130I CCWGG 2 cut(s) 45, 534
BcnI CCSGG 1 cut(s) 677
BcoDI GTCTC 5 cut(s) 6, 369, 470, 628, 749
BfaI CTAG 1 cut(s) 683
BfrI CTTAAG 1 cut(s) 799
BfuAI ACCTGC 1 cut(s) 1006
BisI GCNGC 3 cut(s) 91, 859, 945
BlpI GCTNAGC 1 cut(s) 234
BlsI GCNGC 3 cut(s) 92, 860, 946
BmcAI AGTACT 1 cut(s) 223
Bme1390I CCNGG 3 cut(s) 45, 534, 677
Bme18I GGWCC 3 cut(s) 47, 128, 299
BmgT120I GGNCC 4 cut(s) 47, 128, 299, 449
BmiI GGNNCC 4 cut(s) 538, 595, 763, 927
BmrFI CCNGG 3 cut(s) 45, 534, 677
BmrI ACTGGG 1 cut(s) 505
BmsI GCATC 3 cut(s) 315, 514, 1020
BmuI ACTGGG 1 cut(s) 505
BpiI GAAGAC 2 cut(s) 387, 789
BplI GAGNNNNNCTC 2 cut(s) 746, 778
BpmI CTGGAG 1 cut(s) 463
Bpu1102I GCTNAGC 1 cut(s) 234
BpuMI CCSGG 1 cut(s) 677
BsaHI GRCGYC 1 cut(s) 489
BsaI GGTCTC 3 cut(s) 369, 470, 749
BsaJI CCNNGG 4 cut(s) 43, 355, 388, 579
BsaWI WCCGGW 1 cut(s) 367
BsaXI ACNNNNNCTCC 2 cut(s) 663, 693
Bsc4I CCNNNNNNNGG 4 cut(s) 100, 142, 683, 908
Bse118I RCCGGY 1 cut(s) 758
Bse1I ACTGG 2 cut(s) 500, 565
Bse3DI GCAATG 1 cut(s) 37
BseBI CCWGG 2 cut(s) 45, 534
BseDI CCNNGG 4 cut(s) 43, 355, 388, 579
BseGI GGATG 4 cut(s) 168, 306, 365, 733
BseLI CCNNNNNNNGG 4 cut(s) 100, 142, 683, 908
BseMI GCAATG 1 cut(s) 37
BseMII CTCAG 3 cut(s) 243, 248, 555
BseNI ACTGG 2 cut(s) 500, 565
BseRI GAGGAG 1 cut(s) 754
BseXI GCAGC 3 cut(s) 77, 870, 956
BsgI GTGCAG 1 cut(s) 891
BshFI GGCC 1 cut(s) 451
BshNI GGYRCC 1 cut(s) 593
BsiHKAI GWGCWC 1 cut(s) 170
BsiSI CCGG 3 cut(s) 368, 677, 759
BslFI GGGAC 1 cut(s) 659
BslI CCNNNNNNNGG 4 cut(s) 100, 142, 683, 908
BsmAI GTCTC 5 cut(s) 6, 369, 470, 628, 749
BsmFI GGGAC 1 cut(s) 659
BsnI GGCC 1 cut(s) 451
Bso31I GGTCTC 3 cut(s) 369, 470, 749
Bsp1286I GDGCHC 1 cut(s) 170
Bsp143I GATC 4 cut(s) 262, 438, 585, 925
Bsp1720I GCTNAGC 1 cut(s) 234
BspACI CCGC 5 cut(s) 254, 297, 614, 861, 867
BspANI GGCC 1 cut(s) 451
BspCNI CTCAG 3 cut(s) 242, 247, 554
BspHI TCATGA 1 cut(s) 738
BspLI GGNNCC 4 cut(s) 538, 595, 763, 927
BspMI ACCTGC 1 cut(s) 1006
BspPI GGATC 4 cut(s) 270, 593, 920, 933
BspT107I GGYRCC 1 cut(s) 593
BspTI CTTAAG 1 cut(s) 799
BspTNI GGTCTC 3 cut(s) 369, 470, 749
BsrDI GCAATG 1 cut(s) 37
BsrFI RCCGGY 1 cut(s) 758
BsrI ACTGG 2 cut(s) 500, 565
BssAI RCCGGY 1 cut(s) 758
BssECI CCNNGG 4 cut(s) 43, 355, 388, 579
BssMI GATC 4 cut(s) 262, 438, 585, 925
BssNI GRCGYC 1 cut(s) 489
BssT1I CCWWGG 2 cut(s) 355, 388
Bst2UI CCWGG 2 cut(s) 45, 534
Bst4CI ACNGT 5 cut(s) 172, 593, 720, 903, 1039
BstACI GRCGYC 1 cut(s) 489
BstAFI CTTAAG 1 cut(s) 799
BstC8I GCNNGC 4 cut(s) 88, 256, 747, 796
BstDEI CTNAG 3 cut(s) 229, 234, 541
BstDSI CCRYGG 1 cut(s) 579
BstF5I GGATG 4 cut(s) 168, 306, 365, 733
BstHHI GCGC 2 cut(s) 995, 1003
BstKTI GATC 4 cut(s) 265, 441, 588, 928
BstMAI GTCTC 5 cut(s) 6, 369, 470, 628, 749
BstMBI GATC 4 cut(s) 262, 438, 585, 925
BstMWI GCNNNNNNNGC 2 cut(s) 303, 864
BstNI CCWGG 2 cut(s) 45, 534
BstSCI CCNGG 3 cut(s) 43, 532, 675
BstV1I GCAGC 3 cut(s) 77, 870, 956
BstV2I GAAGAC 2 cut(s) 387, 789
BstX2I RGATCY 1 cut(s) 925
BstYI RGATCY 1 cut(s) 925
BsuRI GGCC 1 cut(s) 451
BtgI CCRYGG 1 cut(s) 579
BtsCI GGATG 4 cut(s) 168, 306, 365, 733
BtsIMutI CAGTG 2 cut(s) 168, 1044
BveI ACCTGC 1 cut(s) 1006
Cac8I GCNNGC 4 cut(s) 88, 256, 747, 796
CaiI CAGNNNCTG 1 cut(s) 62
CciI TCATGA 1 cut(s) 738
CfoI GCGC 2 cut(s) 995, 1003
Cfr10I RCCGGY 1 cut(s) 758
Cfr13I GGNCC 4 cut(s) 47, 128, 299, 449
CseI GACGC 1 cut(s) 35
Csp6I GTAC 3 cut(s) 222, 568, 899
CviAII CATG 6 cut(s) 259, 397, 412, 700, 739, 963
CviQI GTAC 3 cut(s) 222, 568, 899
DdeI CTNAG 3 cut(s) 229, 234, 541
DpnI GATC 4 cut(s) 264, 440, 587, 927
DpnII GATC 4 cut(s) 262, 438, 585, 925
Eco130I CCWWGG 2 cut(s) 355, 388
Eco31I GGTCTC 3 cut(s) 369, 470, 749
Eco47I GGWCC 3 cut(s) 47, 128, 299
Eco57I CTGAAG 1 cut(s) 801
EcoO109I RGGNCCY 2 cut(s) 47, 128
EcoRII CCWGG 2 cut(s) 43, 532
EcoT14I CCWWGG 2 cut(s) 355, 388
ErhI CCWWGG 2 cut(s) 355, 388
FaeI CATG 6 cut(s) 262, 400, 415, 703, 742, 966
FalI AAGNNNNNCTT 6 cut(s) 372, 404, 464, 496, 950, 982
FaqI GGGAC 1 cut(s) 659
FatI CATG 6 cut(s) 258, 396, 411, 699, 738, 962
FauI CCCGC 1 cut(s) 247
FblI GTMKAC 1 cut(s) 492
Fnu4HI GCNGC 3 cut(s) 91, 859, 945
FokI GGATG 4 cut(s) 175, 293, 372, 740
Fsp4HI GCNGC 3 cut(s) 91, 859, 945
FspBI CTAG 1 cut(s) 683
GlaI GCGC 2 cut(s) 994, 1002
GluI GCNGC 3 cut(s) 91, 859, 945
GsuI CTGGAG 1 cut(s) 463
HaeIII GGCC 1 cut(s) 451
HapII CCGG 3 cut(s) 368, 677, 759
HgaI GACGC 1 cut(s) 35
HhaI GCGC 2 cut(s) 995, 1003
Hin1I GRCGYC 1 cut(s) 489
Hin1II CATG 6 cut(s) 262, 400, 415, 703, 742, 966
Hin6I GCGC 2 cut(s) 993, 1001
HinP1I GCGC 2 cut(s) 993, 1001
HindIII AAGCTT 1 cut(s) 105
HinfI GANTC 5 cut(s) 59, 77, 184, 340, 672
HpaII CCGG 3 cut(s) 368, 677, 759
HphI GGTGA 1 cut(s) 335
Hpy166II GTNNAC 1 cut(s) 493
Hpy188I TCNGA 6 cut(s) 58, 76, 279, 427, 544, 641
Hpy188III TCNNGA 7 cut(s) 20, 101, 442, 607, 739, 824, 887
Hpy8I GTNNAC 1 cut(s) 493
Hpy99I CGWCG 1 cut(s) 593
HpyAV CCTTC 4 cut(s) 323, 581, 718, 1041
HpyCH4III ACNGT 5 cut(s) 172, 593, 720, 903, 1039
HpyCH4IV ACGT 3 cut(s) 489, 774, 786
HpyCH4V TGCA 3 cut(s) 660, 872, 939
HpyF10VI GCNNNNNNNGC 2 cut(s) 303, 864
HpyF3I CTNAG 3 cut(s) 229, 234, 541
HpySE526I ACGT 3 cut(s) 489, 774, 786
Hsp92I GRCGYC 1 cut(s) 489
Hsp92II CATG 6 cut(s) 262, 400, 415, 703, 742, 966
HspAI GCGC 2 cut(s) 993, 1001
Kzo9I GATC 4 cut(s) 262, 438, 585, 925
LmnI GCTCC 3 cut(s) 124, 420, 767
Lsp1109I GCAGC 3 cut(s) 77, 870, 956
LweI GCATC 3 cut(s) 315, 514, 1020
MaeI CTAG 1 cut(s) 683
MaeII ACGT 3 cut(s) 489, 774, 786
MaeIII GTNAC 3 cut(s) 350, 604, 775
MalI GATC 4 cut(s) 264, 440, 587, 927
MboI GATC 4 cut(s) 262, 438, 585, 925
MboII GAAGA 2 cut(s) 392, 794
MflI RGATCY 1 cut(s) 925
MhlI GDGCHC 1 cut(s) 170
MlyI GAGTC 1 cut(s) 681
MmeI TCCRAC 1 cut(s) 649
MnlI CCTC 8 cut(s) 16, 60, 153, 440, 550, 775, 857, 964
MseI TTAA 6 cut(s) 174, 482, 771, 800, 986, 1005
MslI CAYNNNNRTG 1 cut(s) 773
MspA1I CMGCKG 2 cut(s) 861, 947
MspCI CTTAAG 1 cut(s) 799
MspI CCGG 3 cut(s) 368, 677, 759
MspR9I CCNGG 3 cut(s) 45, 534, 677
MvaI CCWGG 2 cut(s) 45, 534
MwoI GCNNNNNNNGC 2 cut(s) 303, 864
NciI CCSGG 1 cut(s) 677
NdeII GATC 4 cut(s) 262, 438, 585, 925
NlaIII CATG 6 cut(s) 262, 400, 415, 703, 742, 966
NlaIV GGNNCC 4 cut(s) 538, 595, 763, 927
NmuCI GTSAC 2 cut(s) 604, 775
PagI TCATGA 1 cut(s) 738
PaqCI CACCTGC 1 cut(s) 1006
PfeI GAWTC 4 cut(s) 59, 77, 184, 340
PkrI GCNGC 3 cut(s) 92, 860, 946
PleI GAGTC 1 cut(s) 680
PpsI GAGTC 1 cut(s) 680
PpuMI RGGWCCY 2 cut(s) 47, 128
Psp5II RGGWCCY 2 cut(s) 47, 128
Psp6I CCWGG 2 cut(s) 43, 532
PspGI CCWGG 2 cut(s) 43, 532
PspN4I GGNNCC 4 cut(s) 538, 595, 763, 927
PspPI GGNCC 4 cut(s) 47, 128, 299, 449
PspPPI RGGWCCY 2 cut(s) 47, 128
PstNI CAGNNNCTG 1 cut(s) 62
PsuI RGATCY 1 cut(s) 925
PvuII CAGCTG 1 cut(s) 947
RsaI GTAC 3 cut(s) 223, 569, 900
RsaNI GTAC 3 cut(s) 222, 568, 899
RseI CAYNNNNRTG 1 cut(s) 773
SaqAI TTAA 6 cut(s) 174, 482, 771, 800, 986, 1005
SatI GCNGC 3 cut(s) 91, 859, 945
Sau3AI GATC 4 cut(s) 262, 438, 585, 925
Sau96I GGNCC 4 cut(s) 47, 128, 299, 449
ScaI AGTACT 1 cut(s) 223
SchI GAGTC 1 cut(s) 681
ScrFI CCNGG 3 cut(s) 45, 534, 677
SduI GDGCHC 1 cut(s) 170
SfaNI GCATC 3 cut(s) 315, 514, 1020
SinI GGWCC 3 cut(s) 47, 128, 299
SmiMI CAYNNNNRTG 1 cut(s) 773
SmlI CTYRAG 1 cut(s) 799
SmoI CTYRAG 1 cut(s) 799
SsiI CCGC 5 cut(s) 254, 297, 614, 861, 867
SspMI CTAG 1 cut(s) 683
StyD4I CCNGG 3 cut(s) 43, 532, 675
StyI CCWWGG 2 cut(s) 355, 388
TaaI ACNGT 5 cut(s) 172, 593, 720, 903, 1039
TaiI ACGT 3 cut(s) 492, 777, 789
TaqI TCGA 2 cut(s) 588, 825
TatI WGTACW 1 cut(s) 221
TfiI GAWTC 4 cut(s) 59, 77, 184, 340
Tru1I TTAA 6 cut(s) 174, 482, 771, 800, 986, 1005
Tru9I TTAA 6 cut(s) 174, 482, 771, 800, 986, 1005
TscAI CASTG 2 cut(s) 175, 1044
TseFI GTSAC 2 cut(s) 604, 775
TseI GCWGC 3 cut(s) 90, 858, 944
Tsp45I GTSAC 2 cut(s) 604, 775
TspDTI ATGAA 5 cut(s) 400, 444, 561, 727, 755
TspGWI ACGGA 1 cut(s) 24
TspRI CASTG 2 cut(s) 175, 1044
Vha464I CTTAAG 1 cut(s) 799
VpaK11BI GGWCC 3 cut(s) 47, 128, 299
XcmI CCANNNNNNNNNTGG 1 cut(s) 353
XmiI GTMKAC 1 cut(s) 492
XspI CTAG 1 cut(s) 683
ZraI GACGTC 1 cut(s) 490
ZrmI AGTACT 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.