pycom111g01380

Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. Functions as Fe-S scaffold, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Essential for embryo development

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0012080
Physical Location & Seq
Reverse (-)
887588 .. 888496
909 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g01380.1

Sequence Viewer

Length: 909 bp
ATGGCCACTGTAAAGCATAAGATACTGGTTTTATCAGGAAAAGGTGGAGTTGGCAAGAGCACATTCTCTGCCCAACTTGCATTTGCACTAGCAGCTATGGACTACCAGGTGGGACTTCTTGACATCGATATTTGTGGCCCAAGCATCCCAAAGATGCTCGGCCTAGAGGGCCAAGAAATCCACCAGAGCAACCTTGGCTGGTCTCCTGTCTATGTTGAGCAAAACCTTGGGGTCATGTCAATTGGCTTTATGCTTCCGGATTCTGATGACGCTGTTATATGGAGGGGGCCGCGCAAAAATGCACTCATTAAGCAATTCTTGAAGGATGTTTATTGGGGTGAGCTTGATTTCCTGATAGTGGATGCTCCTCCTGGAACCTCGGATGAGCATATTTCAATTGTCCAATACCTTGATGCTGGCTCAATAGATGGTGCAGTCATAGTCACCACTCCACAACAGGTCTCCATGATTGATGTTCGGAAAGAAGTAAGCTTCTGCAAGAAAGTCGGAGTTCAGGTCCTTGGGGTTGTTGAAAACATGAGTGGTTTGTCCCAGCCAGTTATGGATTTCAAATTTTTGAGGATGACAGAGACCGCTGAGCAAATAGATGTCACGGAATGGGCTAGGGAGCAGCTGAAAGAGAGATTGCCTGAATTCTCAAACATAGTAGCTTTCACCGATGTGTTTGATAGTAGTCGCGGTGGAGCTGAAAGAATGTGTGGGGAAATGGGGGTGGCTTTTCTGGGGAAGGTACCGTTGGACCCGCAGCTTTGTAAGGCTGCCGAAGAAGGTAGATCTTGTTTTGTTGATCAGAAATGTGGGGTGAGTGCACCTGCACTGAAGAAAATCATTGATAAAATGATGAAAAATCAGGGAATATCTACAATGAAGGTTGACAACGATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0042802 GO:0042803 GO:0044424 GO:0044444 GO:0044464 GO:0046983 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ParA

Protein Analysis

303

Amino Acids

32.96

Weight (kDa)

4.98

Isoelectric Point (pI)

31.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ParA PF10609 4 - 187 8e-82 NUBPL iron-transfer P-loop NTPase
ArsA_ATPase PF02374 6 - 52 5.6e-07 Anion-transporting ATPase
AAA_31 PF13614 7 - 125 2.2e-06 AAA domain
CbiA PF01656 8 - 265 1.3e-12 CobQ/CobB/MinD/ParA nucleotide binding domain
ParA PF10609 226 - 287 2e-06 NUBPL iron-transfer P-loop NTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50960
fragaria_vesca FvH4_6g52730
malus_domestica MD09G1017000.v1.1 MD17G1017500.v1.1
prunus_persica Prupe.3G300400_v2.0.a1 Prupe.3G300400_v2.0.a1
pyrus_communis pycom111g01380 pycom17g01410
rosa_chinensis RchiOBHm_Chr2g0174341
rosa_laevigata RLG00000022259
rosa_multiflora Rmu_sc0003649.1_g000032 Rmu_sc0007150.1_g000002
rosa_roxburghii Rroxscaffold_2G00078280
rosa_rugosa Rorug02G0576400
rosa_samantha Rh2AG657300 Rh2BG668300 Rh2CG631400 Rh2DG681900
rosa_wichuraiana Rw2G053830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 841
Acc36I ACCTGC 1 cut(s) 841
Acc65I GGTACC 1 cut(s) 751
AccB1I GGYRCC 1 cut(s) 751
AccII CGCG 2 cut(s) 292, 699
AccIII TCCGGA 1 cut(s) 256
AciI CCGC 4 cut(s) 290, 594, 699, 764
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 572, 653
AcuI CTGAAG 1 cut(s) 860
AfaI GTAC 1 cut(s) 753
AfiI CCNNNNNNNGG 1 cut(s) 358
AgsI TTSAA 4 cut(s) 322, 396, 533, 571
AjnI CCWGG 2 cut(s) 105, 370
AjuI GAANNNNNNNTTGG 2 cut(s) 740, 772
AleI CACNNNNGTG 1 cut(s) 680
AluBI AGCT 7 cut(s) 95, 343, 492, 634, 671, 707, 769
AluI AGCT 7 cut(s) 95, 343, 492, 634, 671, 707, 769
Alw21I GWGCWC 2 cut(s) 62, 832
Alw26I GTCTC 3 cut(s) 207, 466, 584
Alw44I GTGCAC 1 cut(s) 828
Aor13HI TCCGGA 1 cut(s) 256
AoxI GGCC 5 cut(s) 3, 136, 160, 169, 287
ApaLI GTGCAC 1 cut(s) 828
ApeKI GCWGC 4 cut(s) 92, 631, 766, 779
ApoI RAATTY 2 cut(s) 572, 653
Asp718I GGTACC 1 cut(s) 751
AspLEI GCGC 1 cut(s) 294
AspS9I GGNCC 5 cut(s) 137, 169, 287, 517, 760
AsuHPI GGTGA 4 cut(s) 350, 436, 667, 835
AvaII GGWCC 2 cut(s) 517, 760
BaeGI GKGCMC 1 cut(s) 832
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 751
Bbv12I GWGCWC 2 cut(s) 62, 832
BbvI GCAGC 4 cut(s) 104, 643, 766, 778
BccI CCATC 1 cut(s) 422
BcgI CGANNNNNNTGC 2 cut(s) 487, 521
BciT130I CCWGG 2 cut(s) 107, 372
BclI TGATCA 1 cut(s) 808
BcoDI GTCTC 3 cut(s) 207, 466, 584
BfaI CTAG 3 cut(s) 89, 164, 624
BfuAI ACCTGC 1 cut(s) 841
BglI GCCNNNNNGGC 1 cut(s) 168
BglII AGATCT 1 cut(s) 794
BisI GCNGC 5 cut(s) 93, 290, 632, 767, 780
BlpI GCTNAGC 1 cut(s) 597
BlsI GCNGC 5 cut(s) 94, 291, 633, 768, 781
Bme1390I CCNGG 2 cut(s) 107, 372
Bme18I GGWCC 2 cut(s) 517, 760
BmgT120I GGNCC 5 cut(s) 137, 169, 287, 517, 760
BmiI GGNNCC 4 cut(s) 288, 376, 753, 762
BmrFI CCNGG 2 cut(s) 107, 372
BmsI GCATC 5 cut(s) 144, 153, 352, 403, 892
Bpu1102I GCTNAGC 1 cut(s) 597
Bsa29I ATCGAT 1 cut(s) 126
BsaI GGTCTC 3 cut(s) 207, 466, 584
BsaJI CCNNGG 4 cut(s) 193, 226, 378, 520
BsaWI WCCGGW 1 cut(s) 256
BsaXI ACNNNNNCTCC 2 cut(s) 501, 531
Bsc4I CCNNNNNNNGG 1 cut(s) 358
Bse1I ACTGG 2 cut(s) 30, 557
BseAI TCCGGA 1 cut(s) 256
BseBI CCWGG 2 cut(s) 107, 372
BseCI ATCGAT 1 cut(s) 126
BseDI CCNNGG 4 cut(s) 193, 226, 378, 520
BseGI GGATG 5 cut(s) 144, 331, 367, 388, 588
BseLI CCNNNNNNNGG 1 cut(s) 358
BseMII CTCAG 1 cut(s) 588
BseNI ACTGG 2 cut(s) 30, 557
BseRI GAGGAG 1 cut(s) 357
BseSI GKGCMC 1 cut(s) 832
BseXI GCAGC 4 cut(s) 104, 643, 766, 778
BseYI CCCAGC 1 cut(s) 552
BsgI GTGCAG 2 cut(s) 453, 819
Bsh1236I CGCG 2 cut(s) 292, 699
BshFI GGCC 5 cut(s) 5, 138, 162, 171, 289
BshNI GGYRCC 1 cut(s) 751
BshVI ATCGAT 1 cut(s) 126
BsiHKAI GWGCWC 2 cut(s) 62, 832
BsiSI CCGG 1 cut(s) 257
BslFI GGGAC 2 cut(s) 126, 535
BslI CCNNNNNNNGG 1 cut(s) 358
BsmAI GTCTC 3 cut(s) 207, 466, 584
BsmFI GGGAC 2 cut(s) 126, 535
BsnI GGCC 5 cut(s) 5, 138, 162, 171, 289
Bso31I GGTCTC 3 cut(s) 207, 466, 584
Bsp1286I GDGCHC 2 cut(s) 62, 832
Bsp13I TCCGGA 1 cut(s) 256
Bsp143I GATC 2 cut(s) 794, 808
Bsp1720I GCTNAGC 1 cut(s) 597
BspACI CCGC 4 cut(s) 290, 594, 699, 764
BspANI GGCC 5 cut(s) 5, 138, 162, 171, 289
BspCNI CTCAG 1 cut(s) 589
BspDI ATCGAT 1 cut(s) 126
BspEI TCCGGA 1 cut(s) 256
BspFNI CGCG 2 cut(s) 292, 699
BspLI GGNNCC 4 cut(s) 288, 376, 753, 762
BspMI ACCTGC 1 cut(s) 841
BspT107I GGYRCC 1 cut(s) 751
BspTNI GGTCTC 3 cut(s) 207, 466, 584
BsrI ACTGG 2 cut(s) 30, 557
BssECI CCNNGG 4 cut(s) 193, 226, 378, 520
BssMI GATC 2 cut(s) 794, 808
BssT1I CCWWGG 3 cut(s) 193, 226, 520
Bst2UI CCWGG 2 cut(s) 107, 372
Bst4CI ACNGT 2 cut(s) 10, 756
BstC8I GCNNGC 1 cut(s) 418
BstDEI CTNAG 1 cut(s) 597
BstF5I GGATG 5 cut(s) 144, 331, 367, 388, 588
BstFNI CGCG 2 cut(s) 292, 699
BstHHI GCGC 1 cut(s) 294
BstKTI GATC 2 cut(s) 797, 811
BstMAI GTCTC 3 cut(s) 207, 466, 584
BstMBI GATC 2 cut(s) 794, 808
BstMWI GCNNNNNNNGC 4 cut(s) 77, 92, 168, 195
BstNI CCWGG 2 cut(s) 107, 372
BstSCI CCNGG 2 cut(s) 105, 370
BstSLI GKGCMC 1 cut(s) 832
BstUI CGCG 2 cut(s) 292, 699
BstV1I GCAGC 4 cut(s) 104, 643, 766, 778
BstX2I RGATCY 1 cut(s) 794
BstYI RGATCY 1 cut(s) 794
Bsu15I ATCGAT 1 cut(s) 126
BsuRI GGCC 5 cut(s) 5, 138, 162, 171, 289
BsuTUI ATCGAT 1 cut(s) 126
BtsCI GGATG 5 cut(s) 144, 331, 367, 388, 588
BtsIMutI CAGTG 2 cut(s) 6, 836
BveI ACCTGC 1 cut(s) 841
Cac8I GCNNGC 1 cut(s) 418
CfoI GCGC 1 cut(s) 294
Cfr13I GGNCC 5 cut(s) 137, 169, 287, 517, 760
ClaI ATCGAT 1 cut(s) 126
CseI GACGC 1 cut(s) 278
CsiI ACCWGGT 1 cut(s) 105
Csp6I GTAC 1 cut(s) 752
CviAII CATG 3 cut(s) 235, 466, 538
CviQI GTAC 1 cut(s) 752
DdeI CTNAG 1 cut(s) 597
DpnI GATC 2 cut(s) 796, 810
DpnII GATC 2 cut(s) 794, 808
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 3 cut(s) 193, 226, 520
Eco31I GGTCTC 3 cut(s) 207, 466, 584
Eco47I GGWCC 2 cut(s) 517, 760
Eco57I CTGAAG 1 cut(s) 860
EcoO109I RGGNCCY 1 cut(s) 517
EcoRI GAATTC 1 cut(s) 653
EcoRII CCWGG 2 cut(s) 105, 370
EcoT14I CCWWGG 3 cut(s) 193, 226, 520
EcoT22I ATGCAT 1 cut(s) 907
ErhI CCWWGG 3 cut(s) 193, 226, 520
FaeI CATG 3 cut(s) 238, 469, 541
FalI AAGNNNNNCTT 2 cut(s) 302, 334
FaqI GGGAC 2 cut(s) 126, 535
FatI CATG 3 cut(s) 234, 465, 537
FauI CCCGC 1 cut(s) 771
FbaI TGATCA 1 cut(s) 808
Fnu4HI GCNGC 5 cut(s) 93, 290, 632, 767, 780
FokI GGATG 5 cut(s) 131, 338, 374, 395, 595
Fsp4HI GCNGC 5 cut(s) 93, 290, 632, 767, 780
FspBI CTAG 3 cut(s) 89, 164, 624
GlaI GCGC 1 cut(s) 293
GluI GCNGC 5 cut(s) 93, 290, 632, 767, 780
GsaI CCCAGC 1 cut(s) 556
HaeIII GGCC 5 cut(s) 5, 138, 162, 171, 289
HapII CCGG 1 cut(s) 257
HgaI GACGC 1 cut(s) 278
HhaI GCGC 1 cut(s) 294
Hin1II CATG 3 cut(s) 238, 469, 541
Hin6I GCGC 1 cut(s) 292
HinP1I GCGC 1 cut(s) 292
HincII GTYRAC 1 cut(s) 895
HindII GTYRAC 1 cut(s) 895
HindIII AAGCTT 1 cut(s) 490
HinfI GANTC 1 cut(s) 260
HpaII CCGG 1 cut(s) 257
HphI GGTGA 4 cut(s) 350, 436, 667, 835
Hpy166II GTNNAC 2 cut(s) 830, 895
Hpy188I TCNGA 5 cut(s) 265, 382, 480, 509, 813
Hpy188III TCNNGA 5 cut(s) 36, 119, 257, 319, 352
Hpy8I GTNNAC 2 cut(s) 830, 895
HpyAV CCTTC 4 cut(s) 316, 742, 782, 883
HpyCH4III ACNGT 2 cut(s) 10, 756
HpyCH4V TGCA 8 cut(s) 80, 86, 302, 434, 498, 830, 836, 905
HpyF10VI GCNNNNNNNGC 4 cut(s) 77, 92, 168, 195
HpyF3I CTNAG 1 cut(s) 597
Hsp92II CATG 3 cut(s) 238, 469, 541
HspAI GCGC 1 cut(s) 292
Kpn2I TCCGGA 1 cut(s) 256
KpnI GGTACC 1 cut(s) 755
Ksp22I TGATCA 1 cut(s) 808
Kzo9I GATC 2 cut(s) 794, 808
LmnI GCTCC 3 cut(s) 370, 628, 704
Lsp1109I GCAGC 4 cut(s) 104, 643, 766, 778
LweI GCATC 5 cut(s) 144, 153, 352, 403, 892
MabI ACCWGGT 1 cut(s) 105
MaeI CTAG 3 cut(s) 89, 164, 624
MaeIII GTNAC 2 cut(s) 442, 610
MalI GATC 2 cut(s) 796, 810
MboI GATC 2 cut(s) 794, 808
MboII GAAGA 2 cut(s) 797, 853
MfeI CAATTG 2 cut(s) 240, 396
MflI RGATCY 1 cut(s) 794
MhlI GDGCHC 2 cut(s) 62, 832
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 5 cut(s) 240, 314, 396, 572, 653
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 2 cut(s) 487, 738
MnlI CCTC 5 cut(s) 160, 276, 378, 388, 573
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 907
MroI TCCGGA 1 cut(s) 256
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 309
MslI CAYNNNNRTG 1 cut(s) 680
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 596, 634
MspI CCGG 1 cut(s) 257
MspR9I CCNGG 2 cut(s) 107, 372
MunI CAATTG 2 cut(s) 240, 396
MvaI CCWGG 2 cut(s) 107, 372
MvnI CGCG 2 cut(s) 292, 699
MwoI GCNNNNNNNGC 4 cut(s) 77, 92, 168, 195
NdeII GATC 2 cut(s) 794, 808
NlaIII CATG 3 cut(s) 238, 469, 541
NlaIV GGNNCC 4 cut(s) 288, 376, 753, 762
NmeAIII GCCGAG 1 cut(s) 138
NmuCI GTSAC 2 cut(s) 442, 610
NsiI ATGCAT 1 cut(s) 907
OliI CACNNNNGTG 1 cut(s) 680
PaqCI CACCTGC 1 cut(s) 841
PfeI GAWTC 1 cut(s) 260
PfoI TCCNGGA 1 cut(s) 370
PkrI GCNGC 5 cut(s) 94, 291, 633, 768, 781
PpuMI RGGWCCY 1 cut(s) 517
Psp5II RGGWCCY 1 cut(s) 517
Psp6I CCWGG 2 cut(s) 105, 370
PspFI CCCAGC 1 cut(s) 552
PspGI CCWGG 2 cut(s) 105, 370
PspN4I GGNNCC 4 cut(s) 288, 376, 753, 762
PspPI GGNCC 5 cut(s) 137, 169, 287, 517, 760
PspPPI RGGWCCY 1 cut(s) 517
PsuI RGATCY 1 cut(s) 794
PvuII CAGCTG 1 cut(s) 634
RsaI GTAC 1 cut(s) 753
RsaNI GTAC 1 cut(s) 752
RseI CAYNNNNRTG 1 cut(s) 680
SaqAI TTAA 1 cut(s) 309
SatI GCNGC 5 cut(s) 93, 290, 632, 767, 780
Sau3AI GATC 2 cut(s) 794, 808
Sau96I GGNCC 5 cut(s) 137, 169, 287, 517, 760
ScrFI CCNGG 2 cut(s) 107, 372
SduI GDGCHC 2 cut(s) 62, 832
SexAI ACCWGGT 1 cut(s) 105
SfaNI GCATC 5 cut(s) 144, 153, 352, 403, 892
SfiI GGCCNNNNNGGCC 1 cut(s) 168
SinI GGWCC 2 cut(s) 517, 760
SmiMI CAYNNNNRTG 1 cut(s) 680
Sse9I AATT 5 cut(s) 240, 314, 396, 572, 653
SsiI CCGC 4 cut(s) 290, 594, 699, 764
SspMI CTAG 3 cut(s) 89, 164, 624
StyD4I CCNGG 2 cut(s) 105, 370
StyI CCWWGG 3 cut(s) 193, 226, 520
TaaI ACNGT 2 cut(s) 10, 756
TaqI TCGA 1 cut(s) 126
TasI AATT 5 cut(s) 240, 314, 396, 572, 653
TauI GCSGC 1 cut(s) 292
TfiI GAWTC 1 cut(s) 260
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TscAI CASTG 2 cut(s) 13, 843
TseFI GTSAC 2 cut(s) 442, 610
TseI GCWGC 4 cut(s) 92, 631, 766, 779
Tsp45I GTSAC 2 cut(s) 442, 610
TspDTI ATGAA 2 cut(s) 878, 902
TspGWI ACGGA 1 cut(s) 629
TspRI CASTG 2 cut(s) 13, 843
VneI GTGCAC 1 cut(s) 828
VpaK11BI GGWCC 2 cut(s) 517, 760
XapI RAATTY 2 cut(s) 572, 653
XcmI CCANNNNNNNNNTGG 1 cut(s) 191
XspI CTAG 3 cut(s) 89, 164, 624
Zsp2I ATGCAT 1 cut(s) 907
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.