MD09G1017000.v1.1

Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. Functions as Fe-S scaffold, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Essential for embryo development

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0012080
Physical Location & Seq
Reverse (-)
1061799 .. 1063880
2082 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1017000.v1.1.491

Sequence Viewer

Length: 1071 bp
ATGGAGAACGGTGAAATTCCCGAAGACGCCAACGAACATTGCCCGGGGCCGCAATCCGATTCGGCTGGCAAATCCGACGCCTGCGATGGATGCCCGAACCAGCAGATTTGTGCTACCGCTCCCAAGGGCCCCGACCCAGACGTGGTTGCAATTGCAGAAAGGATGGCCACTGTAAAGCATAAGATACTGGTTTTATCAGGAAAAGGTGGAGTTGGCAAGAGCACATTCTCTGCCCAACTTGCATTTGCACTAGCAGCTATGGACTTCCAGGTGGGACTTCTTGATATTGATATTTGTGGCCCGAGCATCCCAAAGATGCTCGGCCTAGAAGGCCAAGAAATCCACCAGAGCAACCTTGGCTGGTCTCCTGTCTATGTTGAGCAAAACCTCGGGGTCATGTCAATTGGCTTTATGCTTCCGGATCCTGATGACGCTGTTATATGGAGGGGACCGCGCAAAAATGCACTCATTAAGCAATTCTTGAAGGATGTTTATTGGGGTGAGCTTGATTTCCTGATAGTGGATGCTCCTCCTGGAACCTCAGACGAGCATATTTCAATTGTCCAATACCTTGATGCTGGCTCAATAGATGGTGCAATCATAGTCACCACTCCACAACAGGTCTCCATGATTGATGTTCGGAAAGAAGTAAGTTTCTGCAAGAAGGTCGGAGTTCAGGTCCTTGGGGTTGTTGAAAACATGAGTGGTTTGTCCCAGCCAGTTATGGATTTCAAGTTTTTGAGGATGACAGAGACCGCTGAGCAAATAGATGTCACAGAATGGGCTAGGGAGCAGCTGAAAGAGAGATTGCCTGAATTCTCAAACATAGTAGCTTTCACCGATGTGTTTGATAGTAGTCGCGGTGGAGCTGAAAGAATGTGTGGGGAAATGGGGGTGGCTTTTCTGGGGAAGGTACCGTTGGACCCGCAGCTTTGTAAGGCTGCCGAAGAAGGTAGATCTTGTTTTGTTGATCAGAAATGTGGGGTGAGTGCACCTGCACTGAAGAAAATCATTGATAAAATGTTGAAAAATCAGGGAATATCTACAATGTTGGTTGACAACGATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0042802 GO:0042803 GO:0044424 GO:0044444 GO:0044464 GO:0046983 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ParA

Protein Analysis

357

Amino Acids

38.45

Weight (kDa)

4.65

Isoelectric Point (pI)

33.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ParA PF10609 58 - 241 3.5e-81 NUBPL iron-transfer P-loop NTPase
ArsA_ATPase PF02374 60 - 97 2.8e-06 Anion-transporting ATPase
AAA_31 PF13614 61 - 178 7.5e-06 AAA domain
CbiA PF01656 62 - 319 7.3e-12 CobQ/CobB/MinD/ParA nucleotide binding domain
ParA PF10609 280 - 341 3.2e-06 NUBPL iron-transfer P-loop NTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50960
fragaria_vesca FvH4_6g52730
malus_domestica MD09G1017000.v1.1 MD17G1017500.v1.1
prunus_persica Prupe.3G300400_v2.0.a1 Prupe.3G300400_v2.0.a1
pyrus_communis pycom111g01380 pycom17g01410
rosa_chinensis RchiOBHm_Chr2g0174341
rosa_laevigata RLG00000022259
rosa_multiflora Rmu_sc0003649.1_g000032 Rmu_sc0007150.1_g000002
rosa_roxburghii Rroxscaffold_2G00078280
rosa_rugosa Rorug02G0576400
rosa_samantha Rh2AG657300 Rh2BG668300 Rh2CG631400 Rh2DG681900
rosa_wichuraiana Rw2G053830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1003
Acc36I ACCTGC 1 cut(s) 1003
Acc65I GGTACC 1 cut(s) 913
AccB1I GGYRCC 1 cut(s) 913
AccBSI CCGCTC 1 cut(s) 119
AccII CGCG 2 cut(s) 454, 861
AccIII TCCGGA 1 cut(s) 418
AciI CCGC 6 cut(s) 50, 117, 452, 756, 861, 926
AclWI GGATC 2 cut(s) 416, 429
AcoI YGGCCR 1 cut(s) 165
AcsI RAATTY 2 cut(s) 15, 815
AcuI CTGAAG 1 cut(s) 1022
AcyI GRCGYC 2 cut(s) 27, 78
AfaI GTAC 1 cut(s) 915
AfiI CCNNNNNNNGG 2 cut(s) 142, 520
AgsI TTSAA 5 cut(s) 484, 558, 695, 733, 1027
AjiI CACGTC 1 cut(s) 142
AjnI CCWGG 2 cut(s) 267, 532
AjuI GAANNNNNNNTTGG 2 cut(s) 902, 934
AleI CACNNNNGTG 1 cut(s) 842
AluBI AGCT 6 cut(s) 257, 505, 796, 833, 869, 931
AluI AGCT 6 cut(s) 257, 505, 796, 833, 869, 931
Alw21I GWGCWC 2 cut(s) 224, 994
Alw26I GTCTC 3 cut(s) 369, 628, 746
Alw44I GTGCAC 1 cut(s) 990
AlwI GGATC 2 cut(s) 416, 429
Ama87I CYCGRG 3 cut(s) 43, 301, 389
Aor13HI TCCGGA 1 cut(s) 418
AoxI GGCC 6 cut(s) 47, 127, 165, 298, 322, 331
ApaI GGGCCC 1 cut(s) 131
ApaLI GTGCAC 1 cut(s) 990
ApeKI GCWGC 4 cut(s) 254, 793, 928, 941
ApoI RAATTY 2 cut(s) 15, 815
Asp718I GGTACC 1 cut(s) 913
AspLEI GCGC 1 cut(s) 456
AspS9I GGNCC 7 cut(s) 47, 127, 128, 299, 449, 679, 922
AsuC2I CCSGG 2 cut(s) 44, 45
AsuHPI GGTGA 5 cut(s) 23, 512, 598, 829, 997
AvaI CYCGRG 3 cut(s) 43, 301, 389
AvaII GGWCC 3 cut(s) 449, 679, 922
BaeGI GKGCMC 2 cut(s) 131, 994
BalI TGGCCA 1 cut(s) 167
BamHI GGATCC 1 cut(s) 421
BanI GGYRCC 1 cut(s) 913
BanII GRGCYC 1 cut(s) 131
BbsI GAAGAC 1 cut(s) 30
Bbv12I GWGCWC 2 cut(s) 224, 994
BbvI GCAGC 4 cut(s) 266, 805, 928, 940
BccI CCATC 3 cut(s) 80, 157, 584
BcgI CGANNNNNNTGC 2 cut(s) 649, 683
BciT130I CCWGG 2 cut(s) 269, 534
BclI TGATCA 1 cut(s) 970
BcnI CCSGG 2 cut(s) 44, 45
BcoDI GTCTC 3 cut(s) 369, 628, 746
BfaI CTAG 3 cut(s) 251, 326, 786
BfuAI ACCTGC 1 cut(s) 1003
BglI GCCNNNNNGGC 1 cut(s) 330
BglII AGATCT 1 cut(s) 956
BisI GCNGC 5 cut(s) 50, 255, 794, 929, 942
BlpI GCTNAGC 1 cut(s) 759
BlsI GCNGC 5 cut(s) 51, 256, 795, 930, 943
Bme1390I CCNGG 4 cut(s) 44, 45, 269, 534
Bme18I GGWCC 3 cut(s) 449, 679, 922
BmeT110I CYCGRG 3 cut(s) 43, 301, 389
BmgBI CACGTC 1 cut(s) 142
BmgT120I GGNCC 7 cut(s) 47, 127, 128, 299, 449, 679, 922
BmiI GGNNCC 8 cut(s) 48, 129, 130, 423, 450, 538, 915, 924
BmrFI CCNGG 4 cut(s) 44, 45, 269, 534
BmsI GCATC 6 cut(s) 80, 306, 315, 514, 565, 1054
BpiI GAAGAC 1 cut(s) 30
Bpu1102I GCTNAGC 1 cut(s) 759
BpuMI CCSGG 2 cut(s) 44, 45
BsaHI GRCGYC 2 cut(s) 27, 78
BsaI GGTCTC 3 cut(s) 369, 628, 746
BsaJI CCNNGG 6 cut(s) 43, 44, 123, 355, 388, 682
BsaWI WCCGGW 1 cut(s) 418
BsaXI ACNNNNNCTCC 2 cut(s) 663, 693
Bsc4I CCNNNNNNNGG 2 cut(s) 142, 520
Bse1I ACTGG 2 cut(s) 192, 719
Bse3DI GCAATG 1 cut(s) 37
BseAI TCCGGA 1 cut(s) 418
BseBI CCWGG 2 cut(s) 269, 534
BseDI CCNNGG 6 cut(s) 43, 44, 123, 355, 388, 682
BseGI GGATG 6 cut(s) 95, 168, 306, 493, 529, 750
BseLI CCNNNNNNNGG 2 cut(s) 142, 520
BseMI GCAATG 1 cut(s) 37
BseMII CTCAG 2 cut(s) 555, 750
BseNI ACTGG 2 cut(s) 192, 719
BseRI GAGGAG 1 cut(s) 519
BseSI GKGCMC 2 cut(s) 131, 994
BseXI GCAGC 4 cut(s) 266, 805, 928, 940
BseYI CCCAGC 1 cut(s) 714
BsgI GTGCAG 1 cut(s) 981
Bsh1236I CGCG 2 cut(s) 454, 861
BshFI GGCC 6 cut(s) 49, 129, 167, 300, 324, 333
BshNI GGYRCC 1 cut(s) 913
BsiHKAI GWGCWC 2 cut(s) 224, 994
BsiHKCI CYCGRG 3 cut(s) 43, 301, 389
BsiSI CCGG 2 cut(s) 44, 419
BslFI GGGAC 3 cut(s) 288, 462, 697
BslI CCNNNNNNNGG 2 cut(s) 142, 520
BsmAI GTCTC 3 cut(s) 369, 628, 746
BsmFI GGGAC 3 cut(s) 288, 462, 697
BsnI GGCC 6 cut(s) 49, 129, 167, 300, 324, 333
Bso31I GGTCTC 3 cut(s) 369, 628, 746
BsoBI CYCGRG 3 cut(s) 43, 301, 389
Bsp120I GGGCCC 1 cut(s) 127
Bsp1286I GDGCHC 3 cut(s) 131, 224, 994
Bsp13I TCCGGA 1 cut(s) 418
Bsp143I GATC 3 cut(s) 421, 956, 970
Bsp1720I GCTNAGC 1 cut(s) 759
BspACI CCGC 6 cut(s) 50, 117, 452, 756, 861, 926
BspANI GGCC 6 cut(s) 49, 129, 167, 300, 324, 333
BspCNI CTCAG 2 cut(s) 554, 751
BspEI TCCGGA 1 cut(s) 418
BspFNI CGCG 2 cut(s) 454, 861
BspLI GGNNCC 8 cut(s) 48, 129, 130, 423, 450, 538, 915, 924
BspMI ACCTGC 1 cut(s) 1003
BspPI GGATC 2 cut(s) 416, 429
BspT107I GGYRCC 1 cut(s) 913
BspTNI GGTCTC 3 cut(s) 369, 628, 746
BsrBI CCGCTC 1 cut(s) 119
BsrDI GCAATG 1 cut(s) 37
BsrI ACTGG 2 cut(s) 192, 719
BssECI CCNNGG 6 cut(s) 43, 44, 123, 355, 388, 682
BssMI GATC 3 cut(s) 421, 956, 970
BssNI GRCGYC 2 cut(s) 27, 78
BssT1I CCWWGG 3 cut(s) 123, 355, 682
Bst2UI CCWGG 2 cut(s) 269, 534
Bst4CI ACNGT 3 cut(s) 11, 172, 918
BstACI GRCGYC 2 cut(s) 27, 78
BstC8I GCNNGC 3 cut(s) 67, 82, 580
BstDEI CTNAG 2 cut(s) 541, 759
BstF5I GGATG 6 cut(s) 95, 168, 306, 493, 529, 750
BstFNI CGCG 2 cut(s) 454, 861
BstHHI GCGC 1 cut(s) 456
BstKTI GATC 3 cut(s) 424, 959, 973
BstMAI GTCTC 3 cut(s) 369, 628, 746
BstMBI GATC 3 cut(s) 421, 956, 970
BstMWI GCNNNNNNNGC 5 cut(s) 90, 239, 254, 330, 357
BstNI CCWGG 2 cut(s) 269, 534
BstSCI CCNGG 4 cut(s) 42, 43, 267, 532
BstSLI GKGCMC 2 cut(s) 131, 994
BstUI CGCG 2 cut(s) 454, 861
BstV1I GCAGC 4 cut(s) 266, 805, 928, 940
BstV2I GAAGAC 1 cut(s) 30
BstX2I RGATCY 2 cut(s) 421, 956
BstYI RGATCY 2 cut(s) 421, 956
BsuRI GGCC 6 cut(s) 49, 129, 167, 300, 324, 333
BtgZI GCGATG 1 cut(s) 99
BtrI CACGTC 1 cut(s) 142
BtsCI GGATG 6 cut(s) 95, 168, 306, 493, 529, 750
BtsIMutI CAGTG 2 cut(s) 168, 998
BveI ACCTGC 1 cut(s) 1003
Cac8I GCNNGC 3 cut(s) 67, 82, 580
CfoI GCGC 1 cut(s) 456
Cfr13I GGNCC 7 cut(s) 47, 127, 128, 299, 449, 679, 922
Cfr9I CCCGGG 1 cut(s) 43
CseI GACGC 3 cut(s) 35, 86, 440
Csp6I GTAC 1 cut(s) 914
CviAII CATG 3 cut(s) 397, 628, 700
CviQI GTAC 1 cut(s) 914
DdeI CTNAG 2 cut(s) 541, 759
DpnI GATC 3 cut(s) 423, 958, 972
DpnII GATC 3 cut(s) 421, 956, 970
EaeI YGGCCR 1 cut(s) 165
Eco130I CCWWGG 3 cut(s) 123, 355, 682
Eco24I GRGCYC 1 cut(s) 131
Eco31I GGTCTC 3 cut(s) 369, 628, 746
Eco47I GGWCC 3 cut(s) 449, 679, 922
Eco57I CTGAAG 1 cut(s) 1022
Eco88I CYCGRG 3 cut(s) 43, 301, 389
EcoO109I RGGNCCY 3 cut(s) 127, 128, 679
EcoRI GAATTC 1 cut(s) 815
EcoRII CCWGG 2 cut(s) 267, 532
EcoT14I CCWWGG 3 cut(s) 123, 355, 682
EcoT22I ATGCAT 1 cut(s) 1069
EcoT38I GRGCYC 1 cut(s) 131
ErhI CCWWGG 3 cut(s) 123, 355, 682
FaeI CATG 3 cut(s) 400, 631, 703
FalI AAGNNNNNCTT 2 cut(s) 464, 496
FaqI GGGAC 3 cut(s) 288, 462, 697
FatI CATG 3 cut(s) 396, 627, 699
FauI CCCGC 1 cut(s) 933
FbaI TGATCA 1 cut(s) 970
Fnu4HI GCNGC 5 cut(s) 50, 255, 794, 929, 942
FokI GGATG 6 cut(s) 102, 175, 293, 500, 536, 757
FriOI GRGCYC 1 cut(s) 131
Fsp4HI GCNGC 5 cut(s) 50, 255, 794, 929, 942
FspBI CTAG 3 cut(s) 251, 326, 786
GlaI GCGC 1 cut(s) 455
GluI GCNGC 5 cut(s) 50, 255, 794, 929, 942
GsaI CCCAGC 1 cut(s) 718
HaeIII GGCC 6 cut(s) 49, 129, 167, 300, 324, 333
HapII CCGG 2 cut(s) 44, 419
HgaI GACGC 3 cut(s) 35, 86, 440
HhaI GCGC 1 cut(s) 456
Hin1I GRCGYC 2 cut(s) 27, 78
Hin1II CATG 3 cut(s) 400, 631, 703
Hin6I GCGC 1 cut(s) 454
HinP1I GCGC 1 cut(s) 454
HincII GTYRAC 1 cut(s) 1057
HindII GTYRAC 1 cut(s) 1057
HinfI GANTC 1 cut(s) 59
HpaII CCGG 2 cut(s) 44, 419
HphI GGTGA 5 cut(s) 23, 512, 598, 829, 997
Hpy166II GTNNAC 2 cut(s) 992, 1057
Hpy188I TCNGA 6 cut(s) 58, 76, 544, 642, 671, 975
Hpy188III TCNNGA 7 cut(s) 20, 198, 281, 419, 425, 481, 514
Hpy8I GTNNAC 2 cut(s) 992, 1057
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 5 cut(s) 323, 478, 658, 904, 944
HpyCH4III ACNGT 3 cut(s) 11, 172, 918
HpyCH4IV ACGT 1 cut(s) 141
HpyF10VI GCNNNNNNNGC 5 cut(s) 90, 239, 254, 330, 357
HpyF3I CTNAG 2 cut(s) 541, 759
HpySE526I ACGT 1 cut(s) 141
Hsp92I GRCGYC 2 cut(s) 27, 78
Hsp92II CATG 3 cut(s) 400, 631, 703
HspAI GCGC 1 cut(s) 454
Kpn2I TCCGGA 1 cut(s) 418
KpnI GGTACC 1 cut(s) 917
Ksp22I TGATCA 1 cut(s) 970
Kzo9I GATC 3 cut(s) 421, 956, 970
LmnI GCTCC 4 cut(s) 124, 532, 790, 866
Lsp1109I GCAGC 4 cut(s) 266, 805, 928, 940
LweI GCATC 6 cut(s) 80, 306, 315, 514, 565, 1054
MaeI CTAG 3 cut(s) 251, 326, 786
MaeII ACGT 1 cut(s) 141
MaeIII GTNAC 2 cut(s) 604, 772
MalI GATC 3 cut(s) 423, 958, 972
MbiI CCGCTC 1 cut(s) 119
MboI GATC 3 cut(s) 421, 956, 970
MboII GAAGA 3 cut(s) 35, 959, 1015
MfeI CAATTG 3 cut(s) 150, 402, 558
MflI RGATCY 2 cut(s) 421, 956
MhlI GDGCHC 3 cut(s) 131, 224, 994
MlsI TGGCCA 1 cut(s) 167
MluCI AATT 6 cut(s) 15, 150, 402, 476, 558, 815
MluNI TGGCCA 1 cut(s) 167
MmeI TCCRAC 3 cut(s) 99, 649, 900
MnlI CCTC 5 cut(s) 398, 438, 540, 550, 735
Mox20I TGGCCA 1 cut(s) 167
Mph1103I ATGCAT 1 cut(s) 1069
MroI TCCGGA 1 cut(s) 418
MscI TGGCCA 1 cut(s) 167
MseI TTAA 1 cut(s) 471
MslI CAYNNNNRTG 1 cut(s) 842
Msp20I TGGCCA 1 cut(s) 167
MspA1I CMGCKG 2 cut(s) 758, 796
MspI CCGG 2 cut(s) 44, 419
MspR9I CCNGG 4 cut(s) 44, 45, 269, 534
MunI CAATTG 3 cut(s) 150, 402, 558
MvaI CCWGG 2 cut(s) 269, 534
MvnI CGCG 2 cut(s) 454, 861
MwoI GCNNNNNNNGC 5 cut(s) 90, 239, 254, 330, 357
NciI CCSGG 2 cut(s) 44, 45
NdeII GATC 3 cut(s) 421, 956, 970
NlaIII CATG 3 cut(s) 400, 631, 703
NlaIV GGNNCC 8 cut(s) 48, 129, 130, 423, 450, 538, 915, 924
NmeAIII GCCGAG 1 cut(s) 300
NmuCI GTSAC 2 cut(s) 604, 772
NsiI ATGCAT 1 cut(s) 1069
OliI CACNNNNGTG 1 cut(s) 842
PaqCI CACCTGC 1 cut(s) 1003
PfeI GAWTC 1 cut(s) 59
PfoI TCCNGGA 1 cut(s) 532
PkrI GCNGC 5 cut(s) 51, 256, 795, 930, 943
PpuMI RGGWCCY 1 cut(s) 679
Psp5II RGGWCCY 1 cut(s) 679
Psp6I CCWGG 2 cut(s) 267, 532
PspFI CCCAGC 1 cut(s) 714
PspGI CCWGG 2 cut(s) 267, 532
PspN4I GGNNCC 8 cut(s) 48, 129, 130, 423, 450, 538, 915, 924
PspOMI GGGCCC 1 cut(s) 127
PspPI GGNCC 7 cut(s) 47, 127, 128, 299, 449, 679, 922
PspPPI RGGWCCY 1 cut(s) 679
PsuI RGATCY 2 cut(s) 421, 956
PvuII CAGCTG 1 cut(s) 796
RsaI GTAC 1 cut(s) 915
RsaNI GTAC 1 cut(s) 914
RseI CAYNNNNRTG 1 cut(s) 842
SaqAI TTAA 1 cut(s) 471
SatI GCNGC 5 cut(s) 50, 255, 794, 929, 942
Sau3AI GATC 3 cut(s) 421, 956, 970
Sau96I GGNCC 7 cut(s) 47, 127, 128, 299, 449, 679, 922
ScrFI CCNGG 4 cut(s) 44, 45, 269, 534
SduI GDGCHC 3 cut(s) 131, 224, 994
SfaNI GCATC 6 cut(s) 80, 306, 315, 514, 565, 1054
SfiI GGCCNNNNNGGCC 1 cut(s) 330
SinI GGWCC 3 cut(s) 449, 679, 922
SmaI CCCGGG 1 cut(s) 45
SmiMI CAYNNNNRTG 1 cut(s) 842
Sse9I AATT 6 cut(s) 15, 150, 402, 476, 558, 815
SsiI CCGC 6 cut(s) 50, 117, 452, 756, 861, 926
SspMI CTAG 3 cut(s) 251, 326, 786
StyD4I CCNGG 4 cut(s) 42, 43, 267, 532
StyI CCWWGG 3 cut(s) 123, 355, 682
TaaI ACNGT 3 cut(s) 11, 172, 918
TaiI ACGT 1 cut(s) 144
TasI AATT 6 cut(s) 15, 150, 402, 476, 558, 815
TauI GCSGC 1 cut(s) 52
TfiI GAWTC 1 cut(s) 59
Tru1I TTAA 1 cut(s) 471
Tru9I TTAA 1 cut(s) 471
TscAI CASTG 2 cut(s) 175, 1005
TseFI GTSAC 2 cut(s) 604, 772
TseI GCWGC 4 cut(s) 254, 793, 928, 941
Tsp45I GTSAC 2 cut(s) 604, 772
TspMI CCCGGG 1 cut(s) 43
TspRI CASTG 2 cut(s) 175, 1005
VneI GTGCAC 1 cut(s) 990
VpaK11BI GGWCC 3 cut(s) 449, 679, 922
XapI RAATTY 2 cut(s) 15, 815
XcmI CCANNNNNNNNNTGG 1 cut(s) 353
XmaI CCCGGG 1 cut(s) 43
XspI CTAG 3 cut(s) 251, 326, 786
Zsp2I ATGCAT 1 cut(s) 1069
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.