FvH4_6g52730

Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. Functions as Fe-S scaffold, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Essential for embryo development

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0012080
Physical Location & Seq
Forward (+)
38747019 .. 38749514
2496 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g52730.t1

Sequence Viewer

Length: 1074 bp
ATGGAGAACGGCCAAGTGCCCGAAAACGCTAACGAACATTGCCCAGGTACTCAATCCGAGTCTGCGGGGAAGGCTGATTCTTGTGAAGGATGCCCCAATCAGCAGATTTGTGCTACTGCTCCGAAGGGTCCTGACCCAGACTTGGTTGCAATTGCAGAAAGAATGGCTACTGTAAAGCATAAGATACTGGTTCTATCAGGGAAAGGTGGAGTTGGTAAGAGCACATTCTCTGCCCAACTTTCATTTGCACTAGCAGCTATGGACTTCCAGGTGGGTCTTTTGGACATCGACATTTGTGGCCCTAGCATCCCAAAGATGCTTGGCCTAGAGGGCCAAGAAATCCACCAGAGCAACCTCGGCTGGTCTCCTGTCTATGTTGAGTCCAACCTTGGGGTTATGTCAATAGGGTTTATGCTTCCAGACCCTGATGACGCAGTTATATGGAGGGGTCCACGAAAGAATGCGCTCATTAAGCAATTCCTCAAGGATGTCGATTGGGGTGAGCTTGATTTTCTTATAGTTGATGCTCCTCCTGGAACCTCAGATGAGCACATTTCAATTGTTCAAGATCTGGGTGCTGCTTCAATAGATGGTGCAATCATTGTCACCACTCCACAACAAGTTTCCCTTATTGATGTGAGGAAAGAAGTAAGCTTCTGCAAGAAAGTAGGAATCCAGGTTATTGGGGTTGTTGAAAATATGAGTGGTTTGCGCCAGCCGATGTTGGCAGCTTCCAAATTTTTGAGGATGACAGAGGCAGGTGAGGAAAAAGATGTTACAGAATGGGTTATAGAGTATGTGAAAGAGAAAGCGCCAGAATTGCTTGATATAATAGCCTTGAATGAAGTGTTTGATAATAGTGGTGGTGGAGCTATAAAAATGTGCAGGGAAATGGGGGTACCCTTCCTGGGGAAGGTGCCGTTGGATCCCGAGCTTTGCAAGGCTGCTGAAGAAGGCAGGTCCTGCTTTGTTGATCAGAAAAGTGGCACGAGCGCACCTGCACTGAAGAAGATCATTGATACATTGATGGAAAATCAGGGCATATCTAGAAATGTTGTAGACAATGATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0042802 GO:0042803 GO:0044424 GO:0044444 GO:0044464 GO:0046983 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ParA

Protein Analysis

358

Amino Acids

38.26

Weight (kDa)

4.65

Isoelectric Point (pI)

33.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ParA PF10609 58 - 243 1.5e-80 NUBPL iron-transfer P-loop NTPase
AAA_31 PF13614 61 - 212 3.6e-06 AAA domain
CbiA PF01656 62 - 320 5.8e-13 CobQ/CobB/MinD/ParA nucleotide binding domain
ParA PF10609 282 - 342 8.8e-07 NUBPL iron-transfer P-loop NTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50960
fragaria_vesca FvH4_6g52730
malus_domestica MD09G1017000.v1.1 MD17G1017500.v1.1
prunus_persica Prupe.3G300400_v2.0.a1 Prupe.3G300400_v2.0.a1
pyrus_communis pycom111g01380 pycom17g01410
rosa_chinensis RchiOBHm_Chr2g0174341
rosa_laevigata RLG00000022259
rosa_multiflora Rmu_sc0003649.1_g000032 Rmu_sc0007150.1_g000002
rosa_roxburghii Rroxscaffold_2G00078280
rosa_rugosa Rorug02G0576400
rosa_samantha Rh2AG657300 Rh2BG668300 Rh2CG631400 Rh2DG681900
rosa_wichuraiana Rw2G053830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 749, 1006
Acc36I ACCTGC 3 cut(s) 749, 948, 1006
Acc65I GGTACC 1 cut(s) 898
AccB1I GGYRCC 2 cut(s) 898, 916
AccI GTMKAC 1 cut(s) 1059
AciI CCGC 1 cut(s) 65
AclWI GGATC 2 cut(s) 920, 933
AcoI YGGCCR 1 cut(s) 10
AcsI RAATTY 1 cut(s) 737
AcuI CTGAAG 2 cut(s) 969, 1025
AfaI GTAC 2 cut(s) 49, 900
AfiI CCNNNNNNNGG 5 cut(s) 142, 390, 908, 909, 913
AgsI TTSAA 5 cut(s) 558, 566, 585, 695, 841
AjnI CCWGG 5 cut(s) 43, 267, 532, 675, 906
AjuI GAANNNNNNNTTGG 2 cut(s) 905, 937
AluBI AGCT 6 cut(s) 257, 505, 654, 731, 872, 934
AluI AGCT 6 cut(s) 257, 505, 654, 731, 872, 934
Alw21I GWGCWC 2 cut(s) 224, 552
Alw26I GTCTC 1 cut(s) 369
AlwI GGATC 2 cut(s) 920, 933
AlwNI CAGNNNCTG 2 cut(s) 425, 963
Ama87I CYCGRG 1 cut(s) 929
AoxI GGCC 4 cut(s) 10, 298, 322, 331
ApeKI GCWGC 4 cut(s) 254, 578, 728, 944
ApoI RAATTY 1 cut(s) 737
Asp718I GGTACC 1 cut(s) 898
AspLEI GCGC 4 cut(s) 466, 714, 814, 995
AspS9I GGNCC 5 cut(s) 128, 299, 331, 449, 960
AsuHPI GGTGA 3 cut(s) 512, 598, 773
AvaI CYCGRG 1 cut(s) 929
AvaII GGWCC 3 cut(s) 128, 449, 960
BaeGI GKGCMC 1 cut(s) 21
BamHI GGATCC 1 cut(s) 925
BanI GGYRCC 2 cut(s) 898, 916
BauI CACGAG 1 cut(s) 988
Bbv12I GWGCWC 2 cut(s) 224, 552
BbvI GCAGC 4 cut(s) 266, 565, 740, 931
BccI CCATC 2 cut(s) 584, 1021
BceAI ACGGC 2 cut(s) 25, 904
BciT130I CCWGG 5 cut(s) 45, 269, 534, 677, 908
BclI TGATCA 1 cut(s) 973
BcoDI GTCTC 1 cut(s) 369
BfaI CTAG 4 cut(s) 251, 303, 326, 1047
BfoI RGCGCY 1 cut(s) 815
BfuAI ACCTGC 3 cut(s) 749, 948, 1006
BglI GCCNNNNNGGC 1 cut(s) 330
BglII AGATCT 1 cut(s) 568
BisI GCNGC 4 cut(s) 255, 579, 729, 945
BlsI GCNGC 4 cut(s) 256, 580, 730, 946
Bme1390I CCNGG 5 cut(s) 45, 269, 534, 677, 908
Bme18I GGWCC 3 cut(s) 128, 449, 960
BmeT110I CYCGRG 1 cut(s) 929
BmgT120I GGNCC 5 cut(s) 128, 299, 331, 449, 960
BmiI GGNNCC 6 cut(s) 129, 450, 538, 900, 918, 927
BmrFI CCNGG 5 cut(s) 45, 269, 534, 677, 908
BmsI GCATC 5 cut(s) 80, 306, 315, 514, 1057
BpuEI CTTGAG 1 cut(s) 467
BsaI GGTCTC 1 cut(s) 369
BsaJI CCNNGG 4 cut(s) 43, 355, 388, 907
Bsc4I CCNNNNNNNGG 5 cut(s) 142, 390, 908, 909, 913
Bse1I ACTGG 1 cut(s) 192
Bse3DI GCAATG 1 cut(s) 37
BseBI CCWGG 5 cut(s) 45, 269, 534, 677, 908
BseDI CCNNGG 4 cut(s) 43, 355, 388, 907
BseGI GGATG 4 cut(s) 95, 306, 493, 753
BseLI CCNNNNNNNGG 5 cut(s) 142, 390, 908, 909, 913
BseMI GCAATG 1 cut(s) 37
BseMII CTCAG 1 cut(s) 555
BseNI ACTGG 1 cut(s) 192
BseRI GAGGAG 1 cut(s) 519
BseSI GKGCMC 1 cut(s) 21
BseXI GCAGC 4 cut(s) 266, 565, 740, 931
BsgI GTGCAG 2 cut(s) 904, 984
BshFI GGCC 4 cut(s) 12, 300, 324, 333
BshNI GGYRCC 2 cut(s) 898, 916
BsiHKAI GWGCWC 2 cut(s) 224, 552
BsiHKCI CYCGRG 1 cut(s) 929
BslI CCNNNNNNNGG 5 cut(s) 142, 390, 908, 909, 913
BsmAI GTCTC 1 cut(s) 369
BsmI GAATGC 1 cut(s) 466
BsnI GGCC 4 cut(s) 12, 300, 324, 333
Bso31I GGTCTC 1 cut(s) 369
BsoBI CYCGRG 1 cut(s) 929
Bsp1286I GDGCHC 3 cut(s) 21, 224, 552
Bsp143I GATC 4 cut(s) 568, 925, 973, 1011
BspACI CCGC 1 cut(s) 65
BspANI GGCC 4 cut(s) 12, 300, 324, 333
BspCNI CTCAG 1 cut(s) 554
BspLI GGNNCC 6 cut(s) 129, 450, 538, 900, 918, 927
BspMI ACCTGC 3 cut(s) 749, 948, 1006
BspPI GGATC 2 cut(s) 920, 933
BspT107I GGYRCC 2 cut(s) 898, 916
BspTNI GGTCTC 1 cut(s) 369
BsrDI GCAATG 1 cut(s) 37
BsrI ACTGG 1 cut(s) 192
BssECI CCNNGG 4 cut(s) 43, 355, 388, 907
BssMI GATC 4 cut(s) 568, 925, 973, 1011
BssSI CACGAG 1 cut(s) 988
BssT1I CCWWGG 1 cut(s) 388
Bst2BI CACGAG 1 cut(s) 988
Bst2UI CCWGG 5 cut(s) 45, 269, 534, 677, 908
Bst4CI ACNGT 1 cut(s) 172
BstAPI GCANNNNNTGC 1 cut(s) 963
BstC8I GCNNGC 1 cut(s) 716
BstDEI CTNAG 1 cut(s) 541
BstENI CCTNNNNNAGG 1 cut(s) 911
BstF5I GGATG 4 cut(s) 95, 306, 493, 753
BstH2I RGCGCY 1 cut(s) 815
BstHHI GCGC 4 cut(s) 466, 714, 814, 995
BstKTI GATC 4 cut(s) 571, 928, 976, 1014
BstMAI GTCTC 1 cut(s) 369
BstMBI GATC 4 cut(s) 568, 925, 973, 1011
BstMWI GCNNNNNNNGC 7 cut(s) 71, 254, 330, 357, 472, 820, 963
BstNI CCWGG 5 cut(s) 45, 269, 534, 677, 908
BstSCI CCNGG 5 cut(s) 43, 267, 532, 675, 906
BstSLI GKGCMC 1 cut(s) 21
BstV1I GCAGC 4 cut(s) 266, 565, 740, 931
BstX2I RGATCY 2 cut(s) 568, 925
BstXI CCANNNNNNTGG 1 cut(s) 683
BstYI RGATCY 2 cut(s) 568, 925
BsuRI GGCC 4 cut(s) 12, 300, 324, 333
BtsCI GGATG 4 cut(s) 95, 306, 493, 753
BtsIMutI CAGTG 1 cut(s) 1001
BveI ACCTGC 3 cut(s) 749, 948, 1006
Cac8I GCNNGC 1 cut(s) 716
CaiI CAGNNNCTG 2 cut(s) 425, 963
CfoI GCGC 4 cut(s) 466, 714, 814, 995
Cfr13I GGNCC 5 cut(s) 128, 299, 331, 449, 960
CseI GACGC 1 cut(s) 440
Csp6I GTAC 2 cut(s) 48, 899
CviQI GTAC 2 cut(s) 48, 899
DdeI CTNAG 1 cut(s) 541
DpnI GATC 4 cut(s) 570, 927, 975, 1013
DpnII GATC 4 cut(s) 568, 925, 973, 1011
EaeI YGGCCR 1 cut(s) 10
Eco130I CCWWGG 1 cut(s) 388
Eco31I GGTCTC 1 cut(s) 369
Eco47I GGWCC 3 cut(s) 128, 449, 960
Eco57I CTGAAG 2 cut(s) 969, 1025
Eco88I CYCGRG 1 cut(s) 929
EcoNI CCTNNNNNAGG 1 cut(s) 911
EcoO109I RGGNCCY 2 cut(s) 128, 960
EcoRII CCWGG 5 cut(s) 43, 267, 532, 675, 906
EcoT14I CCWWGG 1 cut(s) 388
EcoT22I ATGCAT 1 cut(s) 1072
ErhI CCWWGG 1 cut(s) 388
FalI AAGNNNNNCTT 2 cut(s) 612, 644
FauI CCCGC 1 cut(s) 58
FbaI TGATCA 1 cut(s) 973
FblI GTMKAC 1 cut(s) 1059
Fnu4HI GCNGC 4 cut(s) 255, 579, 729, 945
FokI GGATG 4 cut(s) 102, 293, 500, 760
Fsp4HI GCNGC 4 cut(s) 255, 579, 729, 945
FspBI CTAG 4 cut(s) 251, 303, 326, 1047
GlaI GCGC 4 cut(s) 465, 713, 813, 994
GluI GCNGC 4 cut(s) 255, 579, 729, 945
HaeII RGCGCY 1 cut(s) 815
HaeIII GGCC 4 cut(s) 12, 300, 324, 333
HgaI GACGC 1 cut(s) 440
HhaI GCGC 4 cut(s) 466, 714, 814, 995
Hin6I GCGC 4 cut(s) 464, 712, 812, 993
HinP1I GCGC 4 cut(s) 464, 712, 812, 993
HindIII AAGCTT 1 cut(s) 652
HinfI GANTC 4 cut(s) 59, 77, 380, 672
HphI GGTGA 3 cut(s) 512, 598, 773
Hpy166II GTNNAC 2 cut(s) 452, 1060
Hpy188I TCNGA 4 cut(s) 58, 123, 544, 978
Hpy188III TCNNGA 5 cut(s) 131, 419, 566, 929, 1047
Hpy8I GTNNAC 2 cut(s) 452, 1060
HpyAV CCTTC 6 cut(s) 64, 80, 118, 907, 913, 947
HpyCH4III ACNGT 1 cut(s) 172
HpyCH4V TGCA 9 cut(s) 149, 155, 248, 596, 660, 885, 939, 1001, 1070
HpyF10VI GCNNNNNNNGC 7 cut(s) 71, 254, 330, 357, 472, 820, 963
HpyF3I CTNAG 1 cut(s) 541
HspAI GCGC 4 cut(s) 464, 712, 812, 993
KpnI GGTACC 1 cut(s) 902
Ksp22I TGATCA 1 cut(s) 973
Kzo9I GATC 4 cut(s) 568, 925, 973, 1011
LmnI GCTCC 3 cut(s) 124, 532, 869
Lsp1109I GCAGC 4 cut(s) 266, 565, 740, 931
LweI GCATC 5 cut(s) 80, 306, 315, 514, 1057
MaeI CTAG 4 cut(s) 251, 303, 326, 1047
MaeIII GTNAC 2 cut(s) 604, 775
MalI GATC 4 cut(s) 570, 927, 975, 1013
MboI GATC 4 cut(s) 568, 925, 973, 1011
MboII GAAGA 3 cut(s) 962, 1018, 1021
MfeI CAATTG 2 cut(s) 150, 558
MflI RGATCY 2 cut(s) 568, 925
MhlI GDGCHC 3 cut(s) 21, 224, 552
MluCI AATT 5 cut(s) 150, 476, 558, 737, 818
MlyI GAGTC 2 cut(s) 68, 389
MmeI TCCRAC 2 cut(s) 408, 903
Mph1103I ATGCAT 1 cut(s) 1072
MseI TTAA 1 cut(s) 471
MspR9I CCNGG 5 cut(s) 45, 269, 534, 677, 908
MunI CAATTG 2 cut(s) 150, 558
Mva1269I GAATGC 1 cut(s) 466
MvaI CCWGG 5 cut(s) 45, 269, 534, 677, 908
MwoI GCNNNNNNNGC 7 cut(s) 71, 254, 330, 357, 472, 820, 963
NdeII GATC 4 cut(s) 568, 925, 973, 1011
NlaIV GGNNCC 6 cut(s) 129, 450, 538, 900, 918, 927
NmeAIII GCCGAG 1 cut(s) 336
NmuCI GTSAC 1 cut(s) 604
NsiI ATGCAT 1 cut(s) 1072
PaqCI CACCTGC 2 cut(s) 749, 1006
PctI GAATGC 1 cut(s) 466
PfeI GAWTC 2 cut(s) 77, 672
PfoI TCCNGGA 1 cut(s) 532
PkrI GCNGC 4 cut(s) 256, 580, 730, 946
PleI GAGTC 2 cut(s) 67, 388
PpsI GAGTC 2 cut(s) 67, 388
PpuMI RGGWCCY 2 cut(s) 128, 960
Psp5II RGGWCCY 2 cut(s) 128, 960
Psp6I CCWGG 5 cut(s) 43, 267, 532, 675, 906
PspGI CCWGG 5 cut(s) 43, 267, 532, 675, 906
PspN4I GGNNCC 6 cut(s) 129, 450, 538, 900, 918, 927
PspPI GGNCC 5 cut(s) 128, 299, 331, 449, 960
PspPPI RGGWCCY 2 cut(s) 128, 960
PstNI CAGNNNCTG 2 cut(s) 425, 963
PsuI RGATCY 2 cut(s) 568, 925
RsaI GTAC 2 cut(s) 49, 900
RsaNI GTAC 2 cut(s) 48, 899
SaqAI TTAA 1 cut(s) 471
SatI GCNGC 4 cut(s) 255, 579, 729, 945
Sau3AI GATC 4 cut(s) 568, 925, 973, 1011
Sau96I GGNCC 5 cut(s) 128, 299, 331, 449, 960
SchI GAGTC 2 cut(s) 68, 389
ScrFI CCNGG 5 cut(s) 45, 269, 534, 677, 908
SduI GDGCHC 3 cut(s) 21, 224, 552
SfaNI GCATC 5 cut(s) 80, 306, 315, 514, 1057
SfiI GGCCNNNNNGGCC 1 cut(s) 330
SinI GGWCC 3 cut(s) 128, 449, 960
SmlI CTYRAG 1 cut(s) 482
SmoI CTYRAG 1 cut(s) 482
Sse9I AATT 5 cut(s) 150, 476, 558, 737, 818
SsiI CCGC 1 cut(s) 65
SspMI CTAG 4 cut(s) 251, 303, 326, 1047
StyD4I CCNGG 5 cut(s) 43, 267, 532, 675, 906
StyI CCWWGG 1 cut(s) 388
TaaI ACNGT 1 cut(s) 172
TaqI TCGA 2 cut(s) 288, 492
TasI AATT 5 cut(s) 150, 476, 558, 737, 818
TfiI GAWTC 2 cut(s) 77, 672
Tru1I TTAA 1 cut(s) 471
Tru9I TTAA 1 cut(s) 471
TscAI CASTG 1 cut(s) 1008
TseFI GTSAC 1 cut(s) 604
TseI GCWGC 4 cut(s) 254, 578, 728, 944
Tsp45I GTSAC 1 cut(s) 604
TspDTI ATGAA 2 cut(s) 231, 858
TspRI CASTG 1 cut(s) 1008
VpaK11BI GGWCC 3 cut(s) 128, 449, 960
XagI CCTNNNNNAGG 1 cut(s) 911
XapI RAATTY 1 cut(s) 737
XbaI TCTAGA 1 cut(s) 1046
XmiI GTMKAC 1 cut(s) 1059
XspI CTAG 4 cut(s) 251, 303, 326, 1047
Zsp2I ATGCAT 1 cut(s) 1072
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.